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mass spectrometry; polyacrylamide gel electrophoresis; proteins; proteomics
Abstract:
... A sensitive and convenient “visible SYPRO” staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive th ...
Fissidens; bryology; leaves; mosses and liverworts; new species; soil; China; Macau
Abstract:
... Fissidens macaoensis L. Zhang is described as a new species from Macao, South China. The species can be separated from its congeners by a suite of characters, including (1) minute size; (2) subterraneous tubers, composed of one to several cells, growing laterally from mature rhizoids; (3) abundant rhizoidal gemmae found on the surface of the soil and which are developed from subterraneous rhizoids ...
... The rice (Oryza sativa L.) elongated uppermost internode (eui) gene effectively results in a recessive tall plant type. In eui background, we found a mutant 02428ha that exhibited a semidwarf phenotype. Because semidwarf varieties possess high yield potential, this mutant may be useful for hybrid rice breeding. The present study aimed to characterize the dwarfing mechanism in the mutant. Microscop ...
... Subsurface geochemical behavior of As(V) with Fe(II) was studied under strict anoxic conditions. Abiotic reduction of As(V) (0.1 mM) to As(III) by aqueous Fe(II) and sorbed Fe(II) in pH range 5.0-7.0 and Fe(II)aq concentration (0.6-1.2 mM) was investigated along with the effect of As(V) on the oxidation of Fe(II) by dissolved oxygen (DO). Although the reduction was thermodynamically feasible for h ...
... Canola oil is high in oleic acid which is commonly used for food and industrial purposes. To determine adaptability of spring canola (Brassica napus L.) to the High Plains for industrial oil production, 26 irrigated trials were conducted from 2005 to 2008. Trials were divided into five regions—1: 36–37°N 108°W; 2: 39–40°N 101–103°W; 3: 41–42°N 102–103°W; 4: 41–42°N 104°W; 5: 43–44°N 106–108°W. Cul ...
chromium; cobalt; copper; correlation; eutrophication; geochemistry; heavy metals; industrial effluents; industrial wastes; lakes; lead; manganese; mercury; nickel; normal values; nutrients; phosphorus; principal component analysis; rivers; sediments; sewage; strontium; titanium; vanadium; wastewater; zinc; China
Abstract:
... Concentrations of the nutrients (TN and TP), phosphorus fractions and heavy metals (Co, Cr, Cu, Mn, Ni, Pb, Sr, Ti, V, Zn and Hg) in 40 surface sediment samples collected from Taihu Lake, a eutrophic shallow lake in China, were determined. The results showed that the northwest region of the lake possessed higher concentrations of TN and TP, as well as the similar spatial distribution trend in the ...
... A genetic locus, encoding putative acyltransferase, was induced by autoinducers in Corynebacterium glutamicum. The autoinducers were maximally produced by the bacterium after 24 h culture. Those molecules are resistant to proteinase K treatment (300 μg ml−1) for 30 min at 37°C or at 121°C for 15 min, and remained stable after extensive storage at 4°C. Autoinducers in the cell-free culture fluids f ...
adsorption; biotechnology; breeding; engineering; enzymes; metal ions; pollutants; proteins; yeasts
Abstract:
... Cell surface engineering is a promising strategy for the molecular breeding of whole-cell biocatalysts. By using this strategy, yeasts can be constructed by the cell surface display of functional proteins; these yeasts are referred to as arming yeasts. Because reactions using arming yeasts as whole-cell biocatalysts occur on the cell surface, materials that cannot enter the cell can be used as rea ...
Jasus; coasts; demersal fish; ecosystems; geographical variation; latitude; lobsters; multivariate analysis; population dynamics; surveys; temporal variation; South Africa
Abstract:
... Research survey data collected over 24 years (1986–2009) were used to explore long-term changes in demersal fish assemblages on the west coast of South Africa. Differences in spatial (latitude and depth) and temporal (seasonal and annual) factors were examined using multivariate analyses. Fish assemblages are clearly influenced by depth, with a distinct change in the region of the shelf break betw ...
... The physicochemical characteristics, fatty acid and triacylglycerol compositions, DSC profile and UV/vis spectrum of oil extracted from Albizia julibrissin seeds were determined in this study. The oil content and the moisture of the seeds were 10.50% and 1.56%. The free fatty acid, the peroxide value, the p-anisidine value, the saponification value, the iodine value were 2.54%, 6.61mequiv. O₂/kg o ...
Marchantiopsida; bryology; models; Azores; Portugal
Abstract:
... Conocephalum salebrosum Szweykowski, Buczkowska & Odrzykoski, a hygrophytic species, is newly reported for several localities in continental Portugal as well as in Madeira and Azores Islands. Information from studied specimens, and a map showing the actual distribution of this species and its closely related species, C. conicum (L.) Dumort., are provided as the basis for a discussion on the chorol ...
Saccharomyces cerevisiae; cysteine; mutagenesis; mutants; protein secretion; protein synthesis; screening; serine
Abstract:
... PURPOSE OF WORK: Soluble protein expression is an important first step during various types of protein studies. Here, we present the screening strategy of secretable mutant. The strategy aimed to identify those cysteine residues that provoke protein misfolding in the heterologous expression system. Intentional mutagenesis studies should consider the size of the library and the time required for ex ...
aerators; alum; arsenates; arsenic; arsenites; boats; copper sulfate; eutrophication; lakes; metals; mixing; recreation; sediments; summer; traffic; wind; winter; New Jersey
Abstract:
... Elevated concentrations of arsenic (As) occurred during warm months in water from the outlet of Lake Mohawk in northwestern New Jersey. The shallow manmade lake is surrounded by residential development and used for recreation. Eutrophic conditions are addressed by alum and copper sulfate applications and aerators operating in the summer. In September 2005, arsenite was dominant in hypoxic to anoxi ...
Agoseris; Megaptera novaeangliae; Physeter macrocephalus; Tursiops truncatus; data collection; geophysics; species diversity; spring; surveys; winter; Angola; Gulf of Guinea
Abstract:
... The species richness, spatial distribution, seasonality and interspecific associations of cetaceans in tropical oceanic waters between the Gulf of Guinea and Angola were examined using 5 905.3 h of dedicated survey effort collected from 13 platforms of opportunity (geophysical vessels) between 2004 and 2009, and from incidental records. Most effort (87.8%) was recorded in waters >1 000 m deep. A t ...
Salvia miltiorrhiza; root shoot ratio; roots; rosmarinic acid; salvianolic acid; semiarid zones; shoots; traditional medicine; water stress
Abstract:
... The roots of Salvia miltiorrhiza Bunge are widely used in traditional Chinese medicine. The objective of this study was to investigate the effect of drought stress on growth and active constituents composition in S. miltiorrhiza. Three water-stress treatments, including control, medium drought stress and severe drought stress, were applied on the whole growth cycle. The results showed that drought ...
... Dyes derived from natural sources have emerged as an important alternative to synthetic dyes. Therefore, there is a need for developing better solidâliquid extraction techniques for leaching natural colorants from plant materials for applications in plant research, food as well as dyeing industries. The influence of ultrasound on natural colorant extraction from different potential dye yielding ...
... The objectives of the study were to evaluate the effects of defoliation on tiller growth of Poa ligularis Ness. at two different intervals after fire as well as how recovery is affected by different levels of herbaceous interspecific competition during the subsequent growing season. Early post-fire defoliation (6 months after burning) reduced (P<0.05) the height, number of green leaves and total g ...
broiler chickens; veterinary drugs; antibiotics; surveys; animal pathogens; broiler feeding; costs and returns; United States
Abstract:
... We use data from a recent national survey to analyze the use of sub-therapeutic antibiotics (STAs) among broiler growers. We estimate that 42% of growers do not use STAs in their feed or water, and instead rely on a set of other practices, including pathogen testing, expanded sanitary protocols, altered feeding regimens, and Hazard Analysis and Critical Control Point HACCP plans to maintain produc ...
... Catches of sharks and bycatch in large-mesh nets and baited drumlines used by the Queensland Shark Control Program were examined to determine the efficacy of both gear types and assess fishing strategies that minimise their impacts. There were few significant differences in the size of both sharks and bycatch in the two gear types, apart from significantly smaller (p < 0.05) tiger sharks Galeocerd ...
... Whether occurrence of life at the physicochemical extremes results from the entire adaptation of organisms to such settings or it originates from the action of a few genes has been debated for a long time. Recent evidence suggests that a limited number of functions suffice to change the predilection of microorganisms for radically different environmental scenarios. For instance, expression of a fe ...
... The environmental impacts of Boroo gold mine project in Mongolia was evaluated by chemical characterization of trace element concentrations in water, soils and tailing dam sediment samples. The results showed that concentrations of B, Cd, Ni and Se in the water samples were within the accepted levels of the Mongolia water quality standard (MNS4586: 1998). However, the concentrations of Al, As, Cu, ...
anthropogenic activities; arsenic; basins; climatic factors; dissolved oxygen; groundwater; heavy metals; nickel; pH; parent rock; river water; rivers; sediments; water temperature; zinc; China
Abstract:
... The Yarlung Tsangbo (YT) is a large river running across southern Tibet and has significant effects on its lower reaches, the Ganges-Brahmaputra Basin. In order to investigate the geochemical features of the YT, 18 surface sediment samples were collected; ten trace element concentrations were measured for bulk sediments and two fine grain size fractions. Meanwhile, basic physicochemical parameters ...
... Elevated foliar concentrations of glutathione (GSH) are a common stress response and potentially crucial in conferring increased stress tolerance. The present study addressed the following questions: can increased foliar GSH levels be achieved in the short term by applying a stem feeding technique to tree seedlings? If yes, will elevated GSH concentrations provide improved tolerance to the adverse ...
... 293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially ...
dogs; elbows; forelimbs; lameness; musculoskeletal diseases; nervous system diseases; shoulders
Abstract:
... Lameness associated with musculoskeletal disorders of the forelimb accounts for approximately 25 per cent of the authors' canine referral caseload. Lameness due to an underlying neurological disorder is less common and is often associated with weakness and, in some dogs, neurological deficits. Most cases of chronic forelimb lameness of musculoskeletal origin seen at the authors' clinic involve the ...
Agave; Fourier transform infrared spectroscopy; adsorption; chemical composition; energy; flax; gas chromatography; hemp; hybrids; kenaf; leaves; pineapples; scanning electron microscopes; sisal; specific energy; zein
Abstract:
... Inverse gas chromatography (IGC) is a suitable method to determine surface energy of natural fibres when compared to wetting techniques. In the present study, the surface properties of raw and modified lignocellulosic fibres have been investigated by IGC. The fibres chosen for the study were flax, hemp, kenaf, agave, agave hybrid, sisal and pineapple. The treatments used were 4% NaOH and 2% zein. ...
Carcharhinus limbatus; coasts; fatty liver; liver; oils; polyunsaturated fatty acids; sharks; Atlantic Ocean; Gulf of Mexico; Indian Ocean; South Africa
Abstract:
... Shark liver oils are rich in polyunsaturated fatty acids, especially the n3 moieties. Data on the liver fatty acids of sharks from African waters, however, are limited. Liver samples from sharks from the western Indian Ocean off the east coast of South Africa and those from the Atlantic Ocean and Gulf of Mexico were examined. This study reports on the fatty acids found in the total lipid fractions ...
... To determine the structural requirements of arenicin-1 in exerting antifungal activity, a truncated peptide with an N-terminal deletion and a peptide with an Ala substitution for an Arg in the beta-turn region were characterised by comparison to arenicin-1. The antifungal activities of the analogues were 25–50% lower than arenicin-1. Trp fluorescence and circular dichroism spectroscopy showed that ...
Chinese hamsters; animal ovaries; cell engineering; gene expression; mechanism of action; microRNA; phenotype
Abstract:
... The ability of microRNAs to influence gene expression is now recognized as a fundamental layer of regulation within the cell. MicroRNAs have a major impact on most biological processes and have generated considerable interest as potential biomarkers as well as therapeutic or engineering targets. In this review we provide a brief overview of their biogenesis, genomic organization and mode of action ...
... This study explores the usefulness of a frame-based modelling approach in the southern Benguela upwelling ecosystem, with four frames describing observed small pelagic fish dominance patterns. We modelled the dynamics of sardine Sardinops sagax and anchovy Engraulis encrasicolus populations under the influence of the small pelagic fishery and climate variability, performed extensive sensitivity an ...
... Farnesyl diphosphate synthase (FPS; EC 2.5.1.1/EC 2.5.1.10) catalyzes the synthesis of farnesyl diphosphate, a key intermediate in the biosynthesis of sesquiterpenes. This present study described the cloning and characterization of a cDNA encoding FPS from leaves of Michelia chapensis Dandy (designated as McFPS, GenBank accession number: GQ214406) for the first time. McFPS was 1,432 bp and contain ...
... The yeast proteins, Msb3p and Msb4p, are two Ypt/Rab-specific GTPase-activating proteins sharing redundant functions in exocytosis, organization of the actin cytoskeleton, and budding site selection. To see if Msb3p might play an additional, specific role, we first tested the sensitivities of msb3 and msb4 mutant strains to different drugs and then screened a genomic library for multicopy suppress ...
databases; early development; females; males; photographs; whales; Mediterranean Sea
Abstract:
... Errors in analysis using natural marks have been recognised since the early development of mark-recapture techniques. In this nine-year study, the mark types present on Cuvier's beaked whales Ziphius cavirostris in the Mediterranean Sea were categorised in order to assess the reliability of natural marks for long-term photo-identification studies. Mark prevalence, abundance and gain/loss rates amo ...
... Biodegradable polymers, such as poly(lactic acid) (PLA) have attracted a lot of attention in the scientific community recently due to a rapid growth of intensive interest in the global environment for alternatives to petroleum-based polymeric materials. Fatty nitrogen compounds (FNCs), fatty amides (FA), fatty hydroxamic acids (FHA), and carbonyl difatty amides (CDFA), which were synthesized from ...
... Recent genome sequencing of Cellvibrio japonicas revealed the presence of two highly homologous ferulic acid esterases (FAEs), encoded by fee1A and fee1B. In this work, the putative FAE, Fee1B, was successfully cloned and expressed in an E. coli system and the purified enzyme was characterized as a type-D FAE with a pH and temperature optima of 6.5 and 35−40°C, respectively. Additionally, the two ...
... Fine fescues are highly valued cool-season turf species. Nearly all of the U.S. fine fescue seed is produced in Oregon, although little is known about the occurrence of weed seed contaminants in fine fescue seed lots. This study was conducted to assess the diversity and frequency of occurrence of weed seed in chewings [Festuca rubra L. subsp. commutata Gaudin], red [Festuca rubra L. subsp. rubra], ...
... Twenty first century has witnessed remarkable achievements in green technology in material science through the development of biocomposites. Oil palm fiber (OPF) extracted from the empty fruit bunches is proven as a good raw material for biocomposites. The cellulose content of OPF is in the range of 43%–65% and lignin content is in the range of 13%–25%. A compilation of the morphology, chemical co ...
... A supply of oxygen, both for the maintenance of anaesthesia using volatile anaesthetic agents and the support of animals requiring additional oxygen, is an essential part of modern veterinary practice. In most practices, this is provided via oxygen cylinders. In this article, Keith Barrand discusses an alternative – oxygen concentrators – with comparisons to cylinder oxygen use, where appropriate. ...
arteries; brain; central nervous system diseases; cysteine; disease models; genes; mice; mutation; myocytes; pathogenesis; smooth muscle; transgenic animals
Abstract:
... Small vessel diseases (SVDs) of the brain are the leading cause of vascular cognitive impairment and a major contributor to stroke in the human adult, however, their pathogenesis is poorly understood. Dominant mutations in NOTCH3 cause CADASIL, one of the most prevalent inherited cerebral SVDs. The disease gene encodes a transmembrane receptor primarily expressed in smooth muscle cells of small ar ...
Perlidae; insect eggs; new species; Arctic region; Indiana
Abstract:
... Perlesta ouabache sp. n. is described from Indiana, USA. Perlesta ouabache is distinguished from Nearctic congeners by characteristics of the male paraprocts and eggs. ...
Digitaria ciliaris; Setaria viridis; acclimation; antioxidant activity; drought; drought tolerance; gas exchange; irrigation; leaves; lipid peroxidation; membrane permeability; peroxidase; photosynthesis; physiological response; plant adaptation; proline; proteins; soil water content; stomatal conductance; superoxide dismutase; water content; water use efficiency
Abstract:
... To understand physiological acclimation of psammophyte to repeated soil drought and rewatering, two psammophytes (Setaria viridis and Digitaria ciliaris) were subjected to three cycles of soil drought and rewatering. The response process of leaf relative water content (RWC), membrane permeability, lipid peroxidation, gas exchange characteristics, antioxidant enzymes, soluble protein, and free prol ...
... The 1-aminocyclopropane-1-carboxylate oxidase gene (ACO1) was upregulated in rice (Oryza sativa L.) phyAphyBphyC mutants lacking any phytochrome and containing the GCC box element, a binding site for rice ethylene-responsive element binding protein 1 (OsEREBP1), in its promoter region. Since the OsEREBP1-like gene EBL1 (OsEREBP1-LIKE 1) was significantly downregulated in phyAphyBphyC mutants, EBL1 ...
... A pilot-scale plant consisting of an oxidation basin (OB), a neutralization basin (NB), a reaction basin (RB), and a settling basin (SB) was designed and built to conduct pilot-scale experiments. With this system, the effects of aeration and pH on ferrous oxidation and on precipitation of the oxidized products were studied systemically. The results of pilot-scale tests showed that aeration at 300 ...
... Stem rust, caused by Puccinia graminis, is a disease of cereal crops worldwide. In Canada, it is primarily controlled using resistant cultivars. Tracking the virulence structure in the pathogen populations is essential to detect new virulent races that may occur. Surveys of barley (Hordeum vulgare), oat (Avena sativa) and wheat (Triticum aestivum) fields and trap nurseries were conducted to provid ...
United States Environmental Protection Agency; arsenic; cadmium; copper; heavy metals; hydrochloric acid; ionic strength; leaching; lead; mine tailings; mining; nickel; soil; solubility; toxicity; zinc; Korean Peninsula
Abstract:
... This study focused on the evaluation of leaching behaviours for arsenic and heavy metals (Cd, Cu, Ni, Pb and Zn) in soils and tailings contaminated by mining activities. Ten representative mine soils were taken at four representative metal mines in Korea. To evaluate the leaching characteristics of the samples, eight extraction methods were adapted namely 0.1 M HCl, 0.5 M HCl, 1.0 M HCl, 3.0 M HCl ...
adsorbents; adsorption; aluminum oxide; arsenates; arsenic; case studies; ferric hydroxide; ferric oxide; filtration; groundwater; pH; titanium dioxide; New Jersey
Abstract:
... Laboratory and field filtration experiments were conducted to study the effectiveness of As(V) removal for five types of adsorbent media. The media included activated alumina (AA), modified activated alumina (MAA), granular ferric hydroxide (GFH), granular ferric oxide (GFO), and granular titanium dioxide (TiO₂). In laboratory batch and column experiments, the synthetic challenge water was used to ...
... Phytoextraction is a remediation technology with a promising application for removing arsenic (As) from soils and waters. Several plant species were evaluated for their As accumulation capacity in hydroponic culture amended with As. Cucumis sativus (cucumber) displayed the highest tolerance against As among 4 plants tested in this study (corn, wheat, sorghum and cucumber). The germination ratio of ...
autumn; physicochemical properties; environmental factors; gelatinization temperature; seasonal variation; thallus; melting point; agar; Gracilaria; Western Australia
Abstract:
... Seasonality in yield, physical and chemical properties of the native agar from different life stages of Gracilaria cliftonii was investigated over a period of six seasons (autumn 2008–winter 2009). Agar yield and its properties varied as a function of seasons and life stages but there was no significant correlation between environmental factors and agar properties (p > 0.05). Native agar varied fr ...
arsenic; cadmium; cement; copper; durability; gold; guidelines; hazardous waste; heavy metals; leaching; lead; mine tailings; mixing; remediation; silver; slags; solidification; toxic substances; waste management; water content; zinc; Korean Peninsula
Abstract:
... The objective of this study is to examine the solidification of toxic elements in tailings by the use of cement and blast furnace slag. Tailings samples were taken at an Au-Ag mine in Korea. To examine the best mixing ratio of tailings and the mixture of ordinary Portland cement (OPC) and blast furnace slag (SG) of 5:5, 6:6, 7:3, and 8:2, the 7:3 ratio of tailings and OPC+SG was adapted. In additi ...
... A stabilization/solidification (S/S) process was used to immobilize Cu in contaminated soils obtained from two army firing ranges sites (A and B) with total Cu concentrations of 520 and 380 mg/kg, respectively. Both waste oyster shells (WOS) and pretreated oyster shells (POS) were used to immobilize Cu in the contaminated soils. Waste oyster shells passing the #10 mesh and #20 mesh were used for t ...
... We established a bicistronic expression system using an encephalomyocarditis virus (EMCV)-derived internal ribosomal entry site (IRES) element to generate stably transformed Drosophila melanogaster Schneider 2 (S2) cells expressing human rotavirus Wa capsid proteins, VP2 and VP6, for the synthesis of VP2/6 double-layered virus-like particle (DVLP). The EMCV-derived IRES permitted bicistronic trans ...
... To explore the significance of the ascorbate-glutathione cycle under drought stress, the leaves of 2-year-old potted apple (Malus domestica Borkh.) plants were used to investigate the changes of each component of the ascorbate-glutathione cycle as well as the gene expression of dehydroascorbate reductase (DHAR, EC 1.8.5.1), ascorbate peroxidase (APX, EC 1.11.1.11) and glutathione reductase (GR, EC ...
equipment; sediments; specific gravity; urinalysis; urinary tract; urine
Abstract:
... Urinalysis is performed on a daily basis in most practices. It is a simple technique, requires minimal equipment and provides information not only about the urinary tract, but also other body systems. This article highlights how to get the most out of routine urinalysis in the typical practice laboratory. Additional information can be gained by submitting samples to an external laboratory for, for ...
... To extract essential oil for industrial use, the yields and compositions of bark oil during Cinnamomum cassia growth (1–3 years old for the branch bark; 5–12 years old for the stem bark) were determined. The branch bark fraction had a higher essential oil yield than the whole branch. The essential oil yield from branch bark varied within 2.70–3.11% (w/w), while that from stem bark was 0.41–2.61% ( ...
base maps; grazing; public lands; rangelands; surveys; vegetation cover; New Mexico
Abstract:
... Data from New Mexico range survey maps created shortly after the passage of the Taylor Grazing Act in 1934 have been preserved and are being used to document changes in vegetation. The range survey data were collected at the time of a critical shift in rangeland policy and practice in federal lands of the United States. This paper describes the historical context of the post-Taylor range surveying ...
... Raptors are commonly presented to veterinary surgeons in practice and typically comprise injured wild birds, individuals kept for falconry, zoo and conservation exhibits, and captive breeding birds. This article describes the common problems seen in captive and wild birds and outlines the basic approach to dealing with sick birds of prey. It provides practitioners with a basic understanding of the ...
... PURPOSE OF WORK: Our study provides a promising alternative of biomimetic coating which functionalizes the dental implant with adhesion peptides and may be useful for enhancing the bone remodeling around Ti implants. A chimeric peptide consisting of an Arg-Gly-Asp (RGD) sequence (mediating cell adhesion) and a RKLPDA (minTBP-1) sequence (specifically recognizing and binding to Ti substrate) was de ...
Acomys; Paraxerus; biodiversity; cytotypes; fauna; inventories; karyotyping; morphometry; new species; rodents; shrews; small mammals; surveys; taxonomic revisions; villages; Tanzania
Abstract:
... A multidisciplinary approach combining morphology, classical morphometry and cytogenetics allowed to establish an updated taxonomic inventory of small mammals from Kingu Pira in the Selous region (south Tanzania). Among the 229 rodents and 21 shrews that were captured during the fieldwork in Ngarembe village in July 2003, 45 specimens were karyotyped. Concerning rodents, this allowed the descripti ...
Acidithiobacillus thiooxidans; arsenic; bacteria; bioremediation; cadmium; cobalt; copper; energy; heavy metals; iron; lead; soil; sulfur; zinc; Korean Peninsula
Abstract:
... The combination of bioremediation and electrokinetics, termed bioelectrokinetics, has been studied constantly to enhance the removal of organic and inorganic contaminants from soil. The use of the bioleaching process originating from Fe- and/or S-oxidizing bacteria may be a feasible technology for the remediation of heavy metal-contaminated soils. In this study, the bioleaching process driven by i ...
rangelands; pastures; equations; geographical variation; data collection; growth models; rain; rain intensity; hydrologic models; temperature; runoff; climate; Australia
Abstract:
... Sub-daily rainfall intensity has a significant impact on runoff and erosion rates in northern Australian rangelands. However, it has been difficult to include sub-daily rainfall intensity in rangeland biophysical models using historical climate data due to the limited number of pluviograph stations with long-term records. In this paper a new empirical model ('Temperature I15' model) was developed ...
Bivalvia; biotopes; new genus; niches; Bay of Biscay; Europe; Gulf of Guinea; Republic of the Congo
Abstract:
... A new genus in the subfamily Pliocardiinae (Vesicomyidae), Christineconcha n. gen., is established for “Calyptogena” regab Cosel & Olu, 2009. The most characteristic features of Christineconcha n. gen. are a rather thin and elongate shell up to 122 mm in length, a pallial line starting from the ventral margin of the anterior adductor scar, the presence of the inner demibranch only and the absence ...
carbonate minerals; coal; data collection; drainage; groundwater; mining; pH; solubility; temporal variation; trace elements; Brazil
Abstract:
... The concentrations and loadings of major and trace elements in coal mine drainage (CMD) from 49 abandoned mines located in the coal fields of the Brazilian state of Santa Catarina were determined. The CMD sites typically displayed a wide spatial and temporal variability in physical and geochemical conditions. The results of our CMD analyses in Santa Catarina State were used to illustrate that the ...
... Strandings of baleen whales are uncommon in Patagonia, Argentina. We report on a stranding of an individual sei whale Balaenoptera borealis on the east coast of San Antonio (40°43.5′ S, 64°55.8′ W), Río Negro province, Argentina. It was the eighth reported stranding of this species along the Argentinean coastline. The stomach content of the whale was dominated by crustaceans. Three parasite specie ...
... This study was carried out to determine the effect of chilling on both cold-acclimated and non-acclimated chickpea (Cicer arietinum L.) cultivars (Gökçe and Canıtez 87). Chickpea seedlings grown in soil culture for 12 days were subjected to chilling temperatures (2 and 4°C for 12 days) after maintaining in cold-acclimation (10°C, 7 days) or non-acclimation (25°C, 7 days) periods. The lowest values ...
Diplodus; allozymes; basins; cytochrome b; demography; loci; mitochondrial DNA
Abstract:
... To investigate the possible influence of the Siculo-Tunisian Strait on the genetic structure of white seabream Diplodus sargus, 13 polymorphic allozyme loci and a fragment of the cytochrome b mito-chondrial DNA were analysed. Allozyme data indicated a moderate but significant differentiation between some north-eastern (Bizerta, Ghar El Melh Lagoon and Mahdia) and southern (Gabes Gulf and El Biban ...
Food and Drug Administration; bioavailability; cadmium; composts; grain yield; greenhouse experimentation; guidelines; humus; iron; paddy soils; polluted soils; remediation; rice; South Korea
Abstract:
... The cadmium (Cd) content of rice grain grown in metal-contaminated paddy soils near abandoned metal mines in South Korea was found to exceed safety guidelines (0.2 mg Cd kg⁻¹) set by the Korea Food and Drug Administration (KFDA). However, current remediation technologies for heavy metal-contaminated soils have limited application with respect to rice paddy soils. Laboratory and greenhouse experime ...
biomass; fish; models; mortality; sport fishing; surveys; Africa
Abstract:
... The stock status of roman Chrysoblephus laticeps was estimated in the Goukamma, a temperate South African marine protected area (MPA). Standardised catch per unit effort (CPUE) from a controlled angling survey on both sides of the MPA border was employed to extrapolate the CPUE at zero fishing mortality. Converted into biomass, the estimate (61% of unexploited biomass) lay midway between those of ...
... The population of microsclerotia of Verticillium dahliae in soil affects the severity of wilt diseases caused by the fungus. The number of microsclerotia (MS) in soil is usually estimated by colonies recovered from plating of soil samples in various forms on solid media, and this value can be useful in making decisions on disease management. Most protocols, however, lack efficiency in making accur ...
... The objective of the present study was to determine the influence of potassium deprivation on the halophyte species Hordeum maritimum grown in hydroponics for 2 weeks. Treatments were with potassium (+K) or without potassium (−K). Growth, water status, mineral nutrition, parameters of oxidative stress [malondialdehyde (MDA), carbonyl groups (C=O), and hydrogen peroxide concentration (H₂O₂) content ...
Baetidae; claws; color; imagos; new species; nymphs; Brazil
Abstract:
... A new species of Apobaetis is described based on nymphs and male imago, and the male imago of A. fiuzai. Specimens were collected in the state of Amazonas, northern region of Brazil. Apobaetis hamadae sp. n. can be easily distinguished from the other congeners by the following combination of characteristics: male imago: (1) abdominal colour pattern; (2) area between forceps not excavated medially. ...
... Liming materials have been used to immobilize heavy metals in contaminated soils. However, no studies have evaluated the use of eggshell waste as a source of calcium carbonate (CaCO₃) to immobilize both cadmium (Cd) and lead (Pb) in soils. This study was conducted to evaluate the effectiveness of eggshell waste on the immobilization of Cd and Pb and to determine the metal availability following va ...
... Artificial neural network (ANN) analysis of immobilized Candida antarctica lipase B-catalyzed esterification of palmitic acid with xylitol was carried out. Temperature, time, amount of enzyme, amount of molecular sieve, substrate molar ratio and volume of solvent were the six important parameters used as the inputs of the network trained by Levenberg–Marquardt (LM) algorithm. After evaluating diff ...
adults; data collection; models; population size; Indian Ocean; Mauritius
Abstract:
... The annual catch of octopus in the Rodrigues Lagoon, located east/north-east of Mauritius, has dropped by almost half since 1994. This has prompted concern over the resource and has initiated an assessment of the current status of the stock. The limited available biological information suggests that the main pulse of recruitment into the lagoon occurs in the first few months of the year, but that ...
Geobacillus; alpha-glucosidase; chemical reactions; chemical structure; glycolipids; ions; microscopy; nuclear magnetic resonance spectroscopy; ricinoleic acid; sodium
Abstract:
... PURPOSE OF WORK: To explore a novel glycolipid, we performed biochemical reactions using a recombinant α-glucosidase from Geobacillus sp. which shows excellent transglycosylation reaction to hydroxyl groups in a variety of compounds. Two different glycolipids (GL-1 and GL-2) were prepared from ricinoleic acid using a recombinant α-glucosidase from Geobacillus sp. The molecular structure of GL-1 wa ...
... The Sofala Bank (16°–21° S) forms a major discontinuity in the gradients of engraulid distribution along the south-eastern African coast. Although the number of species declines southwards, the density and the catches of species associated with higher turbidity increases within the bank, particularly for Thryssa vitrirostris and a few other Thryssa and Stolephorus species. Thryssa vitrirostris for ...
... Streptococcus agalactiae responds very well to intramammary antibiotic treatment. This allows a system of ‘blitz therapy’ to be used to eliminate these infections. Blitz therapy involves antibiotic intramammary treatment into all four quarters during lactation to eradicate S agalactiae infections. Its use became popular in herds with high cell counts that were caused by S agalactiae. Cell counts h ...
Fusarium avenaceum; Lupinus angustifolius; aggression; cluster analysis; crops; cultivars; genetic markers; genetic relationships; genetic variation; genotype; internal transcribed spacers; mating types; pathogens; phylogeny; random amplified polymorphic DNA technique; root rot; roots; seedlings; sequence analysis; sexual reproduction; translation (genetics); Alberta
Abstract:
... Root rot, caused by Fusarium avenaceum (teleomorph: Gibberella avenacea), is an important disease of lupin (Lupinus angustifolius) and other crops in western Canada. Fusarium isolates collected from infected roots of lupin in central Alberta were characterized using two RAPD primers, three microsatellite-primed PCR markers, diagnostic primers for putative mating type, sequence comparison of the tr ...
Nicotiana benthamiana; Tomato ringspot virus; Vitis; fruit trees; genetic variation; host plants; nucleotide sequences; phylogeny; point mutation; provenance; reverse transcriptase polymerase chain reaction; sequence analysis; small fruits; vineyards; North America
Abstract:
... The 3′ untranslated regions (UTRs) of RNA1 and RNA2 of Tomato ringspot virus (ToRSV) are 1.3 kb in size and virtually identical. In this study, sequences containing most of the 3′ UTRs (1168–1265 bp) were determined from 18 ToRSV isolates collected from fruit trees, small fruits and grapevines in North America. Pairwise nucleotide sequence comparisons with sequences of two isolates available in Ge ...
RJM Crawford; R Altwegg; BJ Barham; PJ Barham; JM Durant; BM Dyer; D Geldenhuys; AB Makhado; L Pichegru; PG Ryan; LG Underhill; L Upfold; J Visagie; LJ Waller; PA Whittington
Engraulis encrasicolus; Sardinops sagax; Spheniscus; adults; anchovies; biomass; breeding; fisheries; penguins; sardines; survival rate; South Africa
Abstract:
... The number of African penguins Spheniscus demersus breeding in South Africa collapsed from about 56 000 pairs in 2001 to some 21 000 pairs in 2009, a loss of 35 000 pairs (>60%) in eight years. This reduced the global population to 26 000 pairs, when including Namibian breeders, and led to classification of the species as Endangered. In South Africa, penguins breed in two regions, the Western Cape ...
case studies; community service; economic impact; government agencies; information systems; models; tourism; Australia
Abstract:
... We report a case study of community involvement in industry and regional development which took a whole-system approach to growing the tourist industry in central Australia. All stages of the study are presented beginning with the initial creation of a systems model of the industry in collaboration with the tourism industry, government agencies and the wider community in Stage 1. The process of en ...
... Comparative morphological and physiological studies were carried out on three species of Malaysian Pogonatum collected from habitats exposed to different light intensities ranging from 28±4 W/m² for P. cirratum subsp. macrophyllum (Dozy & Molk.) Hyvönen, to 230±39 W/m² for P. subtortile (Müll.Hal.) A. Jaeger to 751±45 W/m² for P. neesii (Müll.Hal.) Dozy. Total chlorophyll and beta-carotene content ...
Pseudomonas syringae pv. actinidiae; genetic relationships; kiwifruit; pathovars; random amplified polymorphic DNA technique; Italy; Japan; Korean Peninsula
Abstract:
... Over the last few years, a severe bacterial disease affecting kiwifruit has been reported in Italy. Its causal agent was identified as Pseudomonas syringae pv. actinidiae. For epidemiological purposes, we evaluated the discriminatory power of various PCR-based fingerprinting approaches. Profiles were generated by rep-PCR ((GTG)₅-PCR), IS50-PCR and RAPD, from a wide collection of Italian, Korean an ...
acoustic properties; coproducts; glycerol; jute; plasticizers; polypropylenes; protein concentrates; relative humidity; renewable resources; soy protein isolate; soybeans; tensile strength
Abstract:
... Soyprotein–jute fiber composites developed using water without any chemicals as the plasticizer show much better flexural and tensile properties than polypropylene–jute composites. Co-products of soybean processing such as soy oil, soyprotein concentrate and soy protein isolates are inexpensive, abundantly available and are renewable resources that have been extensively studied as potential matrix ...
... The diet of the bearded goby Sufflogobius bibarbatus was studied at two 48 h stations off the Namibian coast. The inner station (120 m) was characterised by a deep layer of low-oxygen bottom water (<0.5 ml DO l⁻¹ or <10% oxygen saturation) scarcely inhabited by metazoan animal life. Gobies at this station were ∼75 mm total length (TL) and displayed a strong vertical size gradient. The diet of all ...
... Cuphea is a potential new oilseed crop rich in medium-chain fatty acids (C8:0 to C14:0) that may serve as a renewable, biodegradable source of oil for lubricants, motor oil, and aircraft fuel. Impacts of climate and soil environment on cuphea growth and development are not well understood. The objective of this study was to evaluate the influence of climate and soil on growth, seed yield, and seed ...
... Purpose of work Hydrodehalogenation of persistent pollutants, such as the groundwater contaminants trichloroethylene and diatrizoate, are catalyzed by biogenic Pd nanoparticles. As H2 gas supply for the dehalogenation reactions is still the limiting factor, this study examines in situ H2 production in the cathode of a microbial electrolysis cell. In a biogenic Pd nanoparticle (bio-Pd) free microbi ...
... Obstructive disease of the urinary tract of horses is an uncommon but potentially serious disorder. Urolithiasis, the most prevalent form of this disorder, can occur at any location in the urinary tract but is most typically seen in the bladder of male adult horses. The presenting signs of urolithiasis are highly variable and depend on the anatomical location of the obstruction. This article discu ...
protein synthesis; antibodies; Western blotting; gardens; stamens; gene expression regulation; habitats; leaves
Abstract:
... Tissue-specific variation in Hsp70 and Hsp90 expression was studied in vegetative (leaf) and reproductive organs (floral tube, ovary and stamen) of Iris pumila plants originating from a sun-exposed and a shaded natural population, which experienced similar growth conditions in an experimental garden. Western blot analysis revealed the presence of both the Hsps in all examined tissues, but at diffe ...
bryology; geographical distribution; journals; mosses and liverworts; nesting; nests
Abstract:
... The 300 species of British and Irish liverworts and hornworts are classified into 10 groups, based on their recorded presence in 10×10 km grid squares (hectads). A two-stage classification (CLUSTASPEC) initially amalgamated single species into clusters and then systematically removed the smallest clusters, redistributing their species until only 10 clusters remained; in the second stage, species w ...
... The spleen is a critical organ in defence against haemoparasitic diseases like babesiosis. Many in vitro and ex vivo studies have identified splenic cells working in concert to activate mechanisms required for successful resolution of infection. The techniques used in those studies, however, remove cells from the anatomical context in which cell interaction and trafficking take place. In this stud ...
... Extramural studies (EMS) are compulsory for all veterinary students in the UK. The benefits of a strong EMS programme are considerable, and have been clearly set out by the RCVS in its EMS recommendations, policy and guidance notes. Although universities take primary responsibility for delivering ‘Day 1 competencies’ for students, EMS placements provide the ‘real world’ environment in which underg ...
... The effects of arsenic (As) species, such as As(III), As(V) and dimethylarsinic acid (DMA), on the accumulation of As in cucumber (Cucumis sativus), as well as on its growth in a soil mesocosm were evaluated. When Cucumis sativus was cultivated in soils contaminated with 20 and 50 mg/kg of As(III), As(V) or DMA for 40 days, the growth was markedly inhibited by the inorganic As (As(III) and As(V)) ...
... The objective of this investigation was to evaluate some of the physical and mechanical properties of resin-treated plywood type panels manufactured from bamboo strips (Gigantochloa scortechinii). Experimental plybamboo samples were made from low molecular weight phenol formaldehyde (LMwPF) treated bamboo strips. They were exposed to outdoor condition ranging from 1 to 12 months. Modulus of elasti ...
... Four-year-old Gingko (Ginkgo biloba L.) trees were exposed to ambient and elevated concentrations of CO₂ and O₃, and their combination for 1 year, using open-top chambers in Shenyang, China in 2006. Growth parameters and endogenous plant hormones were measured simultaneously over the experiment period. Elevated CO₂ increased leaf area and leaf dry weight but had no effect on shoot length, increase ...
... The roles of nitrogen (N) and phosphorus (P) in controlling vegetation transitions in a degraded semi-arid grassland were investigated in a factorial experiment that combined two initial levels of perennial plant density (low and high), three levels of N (N+, N0 and N-) and two levels of P (P+ and P0). Increased levels of both N and P were achieved by fertiliser addition while sucrose was used to ...
... An efficient and rapid plant regeneration system through somatic embryogenesis was developed using 13-week-old zygotic embryos of oil palm (Elaeis guineensis Jacq.) cv. ‘Tenera'. Zygotic embryos were cultured on MS and N6 media supplemented with 2.0 mg L⁻¹ picloram, 2,4-D and dicamba. The highest embryogenic callus formation (32%) was observed on N6 medium with 2,4-D after 3 month culture on callu ...
... An efficient and reproducible protocol was established for genetic transformation in Jatropha curcas through microprojectile bombardment. Decotyledonated embryos from mature seeds were pre-cultured for 5 days and elongated embryonic axis was subjected to bombardment for the optimization of physical parameters. The frequency of transient gus expression and survival of putative transformants were ta ...
Rhynchostegium; bryology; epiphytes; forests; mosses and liverworts; Iran
Abstract:
... In the course of investigations in the Hyrcanian region of northern Iran, especially of epiphyte vegetation in the Elburz Mountains south of the city of Nowshahr (36°39′N, 51°30′E), 18 moss species that have not been reported from the country earlier were found. Several of the new finds represent major range extensions, or fill gaps in the known distributions of the species, for example, Lescuraea ...
... PURPOSE OF WORK: We have developed a strategy of designing multi-functional extracellular matrix proteins for functionalizing bone tissue engineering scaffolds and other biomedical surfaces to achieve improvements in bone grafting, bone repair and bone regeneration. We developed a novel extracellular matrix protein designed to have a cell adhesive RGD sequence derived from fibronectin and active f ...
... PURPOSE OF WORK: Thymidine is an important precursor in antiviral drugs. We have enhanced thymidine production in E. coli by eliminating the repressors in the transcription of the gene coding for carbamoyl phosphate synthetase. The operon for carbamoyl phosphate synthetase (CarAB) in the thymidine biosynthesis regulatory pathway was derepressed by disrupting three known repressors (purR, pepA and ...