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... PURPOSE OF WORK: Our study provides a promising alternative of biomimetic coating which functionalizes the dental implant with adhesion peptides and may be useful for enhancing the bone remodeling around Ti implants. A chimeric peptide consisting of an Arg-Gly-Asp (RGD) sequence (mediating cell adhesion) and a RKLPDA (minTBP-1) sequence (specifically recognizing and binding to Ti substrate) was de ...
Acomys; Paraxerus; biodiversity; cytotypes; fauna; inventories; karyotyping; morphometry; new species; rodents; shrews; small mammals; surveys; taxonomic revisions; villages; Tanzania
Abstract:
... A multidisciplinary approach combining morphology, classical morphometry and cytogenetics allowed to establish an updated taxonomic inventory of small mammals from Kingu Pira in the Selous region (south Tanzania). Among the 229 rodents and 21 shrews that were captured during the fieldwork in Ngarembe village in July 2003, 45 specimens were karyotyped. Concerning rodents, this allowed the descripti ...
Acidithiobacillus thiooxidans; arsenic; bacteria; bioremediation; cadmium; cobalt; copper; energy; heavy metals; iron; lead; soil; sulfur; zinc; Korean Peninsula
Abstract:
... The combination of bioremediation and electrokinetics, termed bioelectrokinetics, has been studied constantly to enhance the removal of organic and inorganic contaminants from soil. The use of the bioleaching process originating from Fe- and/or S-oxidizing bacteria may be a feasible technology for the remediation of heavy metal-contaminated soils. In this study, the bioleaching process driven by i ...
mass spectrometry; polyacrylamide gel electrophoresis; proteins; proteomics
Abstract:
... A sensitive and convenient “visible SYPRO” staining protocol was developed for visualizing proteins after SDS-PAGE. Gels were sensitized with SYPRO Ruby and then stained with the Coomassie Brilliant Blue G-250 protocol (Blue Silver). This combined protocol had similar or better linearity than staining with only SYPRO Ruby or Blue Silver, respectively. In addition, this method was more sensitive th ...
rangelands; pastures; equations; geographical variation; data collection; growth models; rain; rain intensity; hydrologic models; temperature; runoff; climate; Australia
Abstract:
... Sub-daily rainfall intensity has a significant impact on runoff and erosion rates in northern Australian rangelands. However, it has been difficult to include sub-daily rainfall intensity in rangeland biophysical models using historical climate data due to the limited number of pluviograph stations with long-term records. In this paper a new empirical model ('Temperature I15' model) was developed ...
Bivalvia; biotopes; new genus; niches; Bay of Biscay; Europe; Gulf of Guinea; Republic of the Congo
Abstract:
... A new genus in the subfamily Pliocardiinae (Vesicomyidae), Christineconcha n. gen., is established for “Calyptogena” regab Cosel & Olu, 2009. The most characteristic features of Christineconcha n. gen. are a rather thin and elongate shell up to 122 mm in length, a pallial line starting from the ventral margin of the anterior adductor scar, the presence of the inner demibranch only and the absence ...
Fissidens; bryology; leaves; mosses and liverworts; new species; soil; China; Macau
Abstract:
... Fissidens macaoensis L. Zhang is described as a new species from Macao, South China. The species can be separated from its congeners by a suite of characters, including (1) minute size; (2) subterraneous tubers, composed of one to several cells, growing laterally from mature rhizoids; (3) abundant rhizoidal gemmae found on the surface of the soil and which are developed from subterraneous rhizoids ...
... The rice (Oryza sativa L.) elongated uppermost internode (eui) gene effectively results in a recessive tall plant type. In eui background, we found a mutant 02428ha that exhibited a semidwarf phenotype. Because semidwarf varieties possess high yield potential, this mutant may be useful for hybrid rice breeding. The present study aimed to characterize the dwarfing mechanism in the mutant. Microscop ...
carbonate minerals; coal; data collection; drainage; groundwater; mining; pH; solubility; temporal variation; trace elements; Brazil
Abstract:
... The concentrations and loadings of major and trace elements in coal mine drainage (CMD) from 49 abandoned mines located in the coal fields of the Brazilian state of Santa Catarina were determined. The CMD sites typically displayed a wide spatial and temporal variability in physical and geochemical conditions. The results of our CMD analyses in Santa Catarina State were used to illustrate that the ...
... Strandings of baleen whales are uncommon in Patagonia, Argentina. We report on a stranding of an individual sei whale Balaenoptera borealis on the east coast of San Antonio (40°43.5′ S, 64°55.8′ W), Río Negro province, Argentina. It was the eighth reported stranding of this species along the Argentinean coastline. The stomach content of the whale was dominated by crustaceans. Three parasite specie ...
... Subsurface geochemical behavior of As(V) with Fe(II) was studied under strict anoxic conditions. Abiotic reduction of As(V) (0.1 mM) to As(III) by aqueous Fe(II) and sorbed Fe(II) in pH range 5.0-7.0 and Fe(II)aq concentration (0.6-1.2 mM) was investigated along with the effect of As(V) on the oxidation of Fe(II) by dissolved oxygen (DO). Although the reduction was thermodynamically feasible for h ...
... This study was carried out to determine the effect of chilling on both cold-acclimated and non-acclimated chickpea (Cicer arietinum L.) cultivars (Gökçe and Canıtez 87). Chickpea seedlings grown in soil culture for 12 days were subjected to chilling temperatures (2 and 4°C for 12 days) after maintaining in cold-acclimation (10°C, 7 days) or non-acclimation (25°C, 7 days) periods. The lowest values ...
... Canola oil is high in oleic acid which is commonly used for food and industrial purposes. To determine adaptability of spring canola (Brassica napus L.) to the High Plains for industrial oil production, 26 irrigated trials were conducted from 2005 to 2008. Trials were divided into five regions—1: 36–37°N 108°W; 2: 39–40°N 101–103°W; 3: 41–42°N 102–103°W; 4: 41–42°N 104°W; 5: 43–44°N 106–108°W. Cul ...
Diplodus; allozymes; basins; cytochrome b; demography; loci; mitochondrial DNA
Abstract:
... To investigate the possible influence of the Siculo-Tunisian Strait on the genetic structure of white seabream Diplodus sargus, 13 polymorphic allozyme loci and a fragment of the cytochrome b mito-chondrial DNA were analysed. Allozyme data indicated a moderate but significant differentiation between some north-eastern (Bizerta, Ghar El Melh Lagoon and Mahdia) and southern (Gabes Gulf and El Biban ...
Food and Drug Administration; bioavailability; cadmium; composts; grain yield; greenhouse experimentation; guidelines; humus; iron; paddy soils; polluted soils; remediation; rice; South Korea
Abstract:
... The cadmium (Cd) content of rice grain grown in metal-contaminated paddy soils near abandoned metal mines in South Korea was found to exceed safety guidelines (0.2 mg Cd kg⁻¹) set by the Korea Food and Drug Administration (KFDA). However, current remediation technologies for heavy metal-contaminated soils have limited application with respect to rice paddy soils. Laboratory and greenhouse experime ...
biomass; fish; models; mortality; sport fishing; surveys; Africa
Abstract:
... The stock status of roman Chrysoblephus laticeps was estimated in the Goukamma, a temperate South African marine protected area (MPA). Standardised catch per unit effort (CPUE) from a controlled angling survey on both sides of the MPA border was employed to extrapolate the CPUE at zero fishing mortality. Converted into biomass, the estimate (61% of unexploited biomass) lay midway between those of ...
... The population of microsclerotia of Verticillium dahliae in soil affects the severity of wilt diseases caused by the fungus. The number of microsclerotia (MS) in soil is usually estimated by colonies recovered from plating of soil samples in various forms on solid media, and this value can be useful in making decisions on disease management. Most protocols, however, lack efficiency in making accur ...
... The objective of the present study was to determine the influence of potassium deprivation on the halophyte species Hordeum maritimum grown in hydroponics for 2 weeks. Treatments were with potassium (+K) or without potassium (−K). Growth, water status, mineral nutrition, parameters of oxidative stress [malondialdehyde (MDA), carbonyl groups (C=O), and hydrogen peroxide concentration (H₂O₂) content ...
Baetidae; claws; color; imagos; new species; nymphs; Brazil
Abstract:
... A new species of Apobaetis is described based on nymphs and male imago, and the male imago of A. fiuzai. Specimens were collected in the state of Amazonas, northern region of Brazil. Apobaetis hamadae sp. n. can be easily distinguished from the other congeners by the following combination of characteristics: male imago: (1) abdominal colour pattern; (2) area between forceps not excavated medially. ...
... Liming materials have been used to immobilize heavy metals in contaminated soils. However, no studies have evaluated the use of eggshell waste as a source of calcium carbonate (CaCO₃) to immobilize both cadmium (Cd) and lead (Pb) in soils. This study was conducted to evaluate the effectiveness of eggshell waste on the immobilization of Cd and Pb and to determine the metal availability following va ...
... Artificial neural network (ANN) analysis of immobilized Candida antarctica lipase B-catalyzed esterification of palmitic acid with xylitol was carried out. Temperature, time, amount of enzyme, amount of molecular sieve, substrate molar ratio and volume of solvent were the six important parameters used as the inputs of the network trained by Levenberg–Marquardt (LM) algorithm. After evaluating diff ...
adults; data collection; models; population size; Indian Ocean; Mauritius
Abstract:
... The annual catch of octopus in the Rodrigues Lagoon, located east/north-east of Mauritius, has dropped by almost half since 1994. This has prompted concern over the resource and has initiated an assessment of the current status of the stock. The limited available biological information suggests that the main pulse of recruitment into the lagoon occurs in the first few months of the year, but that ...
chromium; cobalt; copper; correlation; eutrophication; geochemistry; heavy metals; industrial effluents; industrial wastes; lakes; lead; manganese; mercury; nickel; normal values; nutrients; phosphorus; principal component analysis; rivers; sediments; sewage; strontium; titanium; vanadium; wastewater; zinc; China
Abstract:
... Concentrations of the nutrients (TN and TP), phosphorus fractions and heavy metals (Co, Cr, Cu, Mn, Ni, Pb, Sr, Ti, V, Zn and Hg) in 40 surface sediment samples collected from Taihu Lake, a eutrophic shallow lake in China, were determined. The results showed that the northwest region of the lake possessed higher concentrations of TN and TP, as well as the similar spatial distribution trend in the ...
... A genetic locus, encoding putative acyltransferase, was induced by autoinducers in Corynebacterium glutamicum. The autoinducers were maximally produced by the bacterium after 24 h culture. Those molecules are resistant to proteinase K treatment (300 μg ml−1) for 30 min at 37°C or at 121°C for 15 min, and remained stable after extensive storage at 4°C. Autoinducers in the cell-free culture fluids f ...
Geobacillus; alpha-glucosidase; chemical reactions; chemical structure; glycolipids; ions; microscopy; nuclear magnetic resonance spectroscopy; ricinoleic acid; sodium
Abstract:
... PURPOSE OF WORK: To explore a novel glycolipid, we performed biochemical reactions using a recombinant α-glucosidase from Geobacillus sp. which shows excellent transglycosylation reaction to hydroxyl groups in a variety of compounds. Two different glycolipids (GL-1 and GL-2) were prepared from ricinoleic acid using a recombinant α-glucosidase from Geobacillus sp. The molecular structure of GL-1 wa ...
... The Sofala Bank (16°–21° S) forms a major discontinuity in the gradients of engraulid distribution along the south-eastern African coast. Although the number of species declines southwards, the density and the catches of species associated with higher turbidity increases within the bank, particularly for Thryssa vitrirostris and a few other Thryssa and Stolephorus species. Thryssa vitrirostris for ...
... Streptococcus agalactiae responds very well to intramammary antibiotic treatment. This allows a system of ‘blitz therapy’ to be used to eliminate these infections. Blitz therapy involves antibiotic intramammary treatment into all four quarters during lactation to eradicate S agalactiae infections. Its use became popular in herds with high cell counts that were caused by S agalactiae. Cell counts h ...
adsorption; biotechnology; breeding; engineering; enzymes; metal ions; pollutants; proteins; yeasts
Abstract:
... Cell surface engineering is a promising strategy for the molecular breeding of whole-cell biocatalysts. By using this strategy, yeasts can be constructed by the cell surface display of functional proteins; these yeasts are referred to as arming yeasts. Because reactions using arming yeasts as whole-cell biocatalysts occur on the cell surface, materials that cannot enter the cell can be used as rea ...
Jasus; coasts; demersal fish; ecosystems; geographical variation; latitude; lobsters; multivariate analysis; population dynamics; surveys; temporal variation; South Africa
Abstract:
... Research survey data collected over 24 years (1986–2009) were used to explore long-term changes in demersal fish assemblages on the west coast of South Africa. Differences in spatial (latitude and depth) and temporal (seasonal and annual) factors were examined using multivariate analyses. Fish assemblages are clearly influenced by depth, with a distinct change in the region of the shelf break betw ...
... The physicochemical characteristics, fatty acid and triacylglycerol compositions, DSC profile and UV/vis spectrum of oil extracted from Albizia julibrissin seeds were determined in this study. The oil content and the moisture of the seeds were 10.50% and 1.56%. The free fatty acid, the peroxide value, the p-anisidine value, the saponification value, the iodine value were 2.54%, 6.61mequiv. O₂/kg o ...
Fusarium avenaceum; Lupinus angustifolius; aggression; cluster analysis; crops; cultivars; genetic markers; genetic relationships; genetic variation; genotype; internal transcribed spacers; mating types; pathogens; phylogeny; random amplified polymorphic DNA technique; root rot; roots; seedlings; sequence analysis; sexual reproduction; translation (genetics); Alberta
Abstract:
... Root rot, caused by Fusarium avenaceum (teleomorph: Gibberella avenacea), is an important disease of lupin (Lupinus angustifolius) and other crops in western Canada. Fusarium isolates collected from infected roots of lupin in central Alberta were characterized using two RAPD primers, three microsatellite-primed PCR markers, diagnostic primers for putative mating type, sequence comparison of the tr ...
Nicotiana benthamiana; Tomato ringspot virus; Vitis; fruit trees; genetic variation; host plants; nucleotide sequences; phylogeny; point mutation; provenance; reverse transcriptase polymerase chain reaction; sequence analysis; small fruits; vineyards; North America
Abstract:
... The 3′ untranslated regions (UTRs) of RNA1 and RNA2 of Tomato ringspot virus (ToRSV) are 1.3 kb in size and virtually identical. In this study, sequences containing most of the 3′ UTRs (1168–1265 bp) were determined from 18 ToRSV isolates collected from fruit trees, small fruits and grapevines in North America. Pairwise nucleotide sequence comparisons with sequences of two isolates available in Ge ...
RJM Crawford; R Altwegg; BJ Barham; PJ Barham; JM Durant; BM Dyer; D Geldenhuys; AB Makhado; L Pichegru; PG Ryan; LG Underhill; L Upfold; J Visagie; LJ Waller; PA Whittington
Engraulis encrasicolus; Sardinops sagax; Spheniscus; adults; anchovies; biomass; breeding; fisheries; penguins; sardines; survival rate; South Africa
Abstract:
... The number of African penguins Spheniscus demersus breeding in South Africa collapsed from about 56 000 pairs in 2001 to some 21 000 pairs in 2009, a loss of 35 000 pairs (>60%) in eight years. This reduced the global population to 26 000 pairs, when including Namibian breeders, and led to classification of the species as Endangered. In South Africa, penguins breed in two regions, the Western Cape ...
case studies; community service; economic impact; government agencies; information systems; models; tourism; Australia
Abstract:
... We report a case study of community involvement in industry and regional development which took a whole-system approach to growing the tourist industry in central Australia. All stages of the study are presented beginning with the initial creation of a systems model of the industry in collaboration with the tourism industry, government agencies and the wider community in Stage 1. The process of en ...
... Comparative morphological and physiological studies were carried out on three species of Malaysian Pogonatum collected from habitats exposed to different light intensities ranging from 28±4 W/m² for P. cirratum subsp. macrophyllum (Dozy & Molk.) Hyvönen, to 230±39 W/m² for P. subtortile (Müll.Hal.) A. Jaeger to 751±45 W/m² for P. neesii (Müll.Hal.) Dozy. Total chlorophyll and beta-carotene content ...
Pseudomonas syringae pv. actinidiae; genetic relationships; kiwifruit; pathovars; random amplified polymorphic DNA technique; Italy; Japan; Korean Peninsula
Abstract:
... Over the last few years, a severe bacterial disease affecting kiwifruit has been reported in Italy. Its causal agent was identified as Pseudomonas syringae pv. actinidiae. For epidemiological purposes, we evaluated the discriminatory power of various PCR-based fingerprinting approaches. Profiles were generated by rep-PCR ((GTG)₅-PCR), IS50-PCR and RAPD, from a wide collection of Italian, Korean an ...
acoustic properties; coproducts; glycerol; jute; plasticizers; polypropylenes; protein concentrates; relative humidity; renewable resources; soy protein isolate; soybeans; tensile strength
Abstract:
... Soyprotein–jute fiber composites developed using water without any chemicals as the plasticizer show much better flexural and tensile properties than polypropylene–jute composites. Co-products of soybean processing such as soy oil, soyprotein concentrate and soy protein isolates are inexpensive, abundantly available and are renewable resources that have been extensively studied as potential matrix ...
Marchantiopsida; bryology; models; Azores; Portugal
Abstract:
... Conocephalum salebrosum Szweykowski, Buczkowska & Odrzykoski, a hygrophytic species, is newly reported for several localities in continental Portugal as well as in Madeira and Azores Islands. Information from studied specimens, and a map showing the actual distribution of this species and its closely related species, C. conicum (L.) Dumort., are provided as the basis for a discussion on the chorol ...
... The diet of the bearded goby Sufflogobius bibarbatus was studied at two 48 h stations off the Namibian coast. The inner station (120 m) was characterised by a deep layer of low-oxygen bottom water (<0.5 ml DO l⁻¹ or <10% oxygen saturation) scarcely inhabited by metazoan animal life. Gobies at this station were ∼75 mm total length (TL) and displayed a strong vertical size gradient. The diet of all ...
... Cuphea is a potential new oilseed crop rich in medium-chain fatty acids (C8:0 to C14:0) that may serve as a renewable, biodegradable source of oil for lubricants, motor oil, and aircraft fuel. Impacts of climate and soil environment on cuphea growth and development are not well understood. The objective of this study was to evaluate the influence of climate and soil on growth, seed yield, and seed ...
Saccharomyces cerevisiae; cysteine; mutagenesis; mutants; protein secretion; protein synthesis; screening; serine
Abstract:
... PURPOSE OF WORK: Soluble protein expression is an important first step during various types of protein studies. Here, we present the screening strategy of secretable mutant. The strategy aimed to identify those cysteine residues that provoke protein misfolding in the heterologous expression system. Intentional mutagenesis studies should consider the size of the library and the time required for ex ...
... Purpose of work Hydrodehalogenation of persistent pollutants, such as the groundwater contaminants trichloroethylene and diatrizoate, are catalyzed by biogenic Pd nanoparticles. As H2 gas supply for the dehalogenation reactions is still the limiting factor, this study examines in situ H2 production in the cathode of a microbial electrolysis cell. In a biogenic Pd nanoparticle (bio-Pd) free microbi ...
... Obstructive disease of the urinary tract of horses is an uncommon but potentially serious disorder. Urolithiasis, the most prevalent form of this disorder, can occur at any location in the urinary tract but is most typically seen in the bladder of male adult horses. The presenting signs of urolithiasis are highly variable and depend on the anatomical location of the obstruction. This article discu ...
protein synthesis; antibodies; Western blotting; gardens; stamens; gene expression regulation; habitats; leaves
Abstract:
... Tissue-specific variation in Hsp70 and Hsp90 expression was studied in vegetative (leaf) and reproductive organs (floral tube, ovary and stamen) of Iris pumila plants originating from a sun-exposed and a shaded natural population, which experienced similar growth conditions in an experimental garden. Western blot analysis revealed the presence of both the Hsps in all examined tissues, but at diffe ...
aerators; alum; arsenates; arsenic; arsenites; boats; copper sulfate; eutrophication; lakes; metals; mixing; recreation; sediments; summer; traffic; wind; winter; New Jersey
Abstract:
... Elevated concentrations of arsenic (As) occurred during warm months in water from the outlet of Lake Mohawk in northwestern New Jersey. The shallow manmade lake is surrounded by residential development and used for recreation. Eutrophic conditions are addressed by alum and copper sulfate applications and aerators operating in the summer. In September 2005, arsenite was dominant in hypoxic to anoxi ...
Agoseris; Megaptera novaeangliae; Physeter macrocephalus; Tursiops truncatus; data collection; geophysics; species diversity; spring; surveys; winter; Angola; Gulf of Guinea
Abstract:
... The species richness, spatial distribution, seasonality and interspecific associations of cetaceans in tropical oceanic waters between the Gulf of Guinea and Angola were examined using 5 905.3 h of dedicated survey effort collected from 13 platforms of opportunity (geophysical vessels) between 2004 and 2009, and from incidental records. Most effort (87.8%) was recorded in waters >1 000 m deep. A t ...
bryology; geographical distribution; journals; mosses and liverworts; nesting; nests
Abstract:
... The 300 species of British and Irish liverworts and hornworts are classified into 10 groups, based on their recorded presence in 10×10 km grid squares (hectads). A two-stage classification (CLUSTASPEC) initially amalgamated single species into clusters and then systematically removed the smallest clusters, redistributing their species until only 10 clusters remained; in the second stage, species w ...
... The spleen is a critical organ in defence against haemoparasitic diseases like babesiosis. Many in vitro and ex vivo studies have identified splenic cells working in concert to activate mechanisms required for successful resolution of infection. The techniques used in those studies, however, remove cells from the anatomical context in which cell interaction and trafficking take place. In this stud ...
... Extramural studies (EMS) are compulsory for all veterinary students in the UK. The benefits of a strong EMS programme are considerable, and have been clearly set out by the RCVS in its EMS recommendations, policy and guidance notes. Although universities take primary responsibility for delivering ‘Day 1 competencies’ for students, EMS placements provide the ‘real world’ environment in which underg ...
... The effects of arsenic (As) species, such as As(III), As(V) and dimethylarsinic acid (DMA), on the accumulation of As in cucumber (Cucumis sativus), as well as on its growth in a soil mesocosm were evaluated. When Cucumis sativus was cultivated in soils contaminated with 20 and 50 mg/kg of As(III), As(V) or DMA for 40 days, the growth was markedly inhibited by the inorganic As (As(III) and As(V)) ...