PubAg

Main content area

You searched for: Source 2015 v.405 Remove constraint Source: 2015 v.405
Start Over

Search

23  Search Results

1 - 23 of 23
Number of results to display per page

Search Results

1. Cardiotonic steroid ouabain stimulates expression of blood–testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells

Author:
Dietze, Raimund; Shihan, Mazen; Stammler, Angelika; Konrad, Lutz; Scheiner-Bobis, Georgios
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 1-13
ISSN:
0303-7207
Subject:
Sertoli cells; dextran; male effect; male fertility; ouabain; rats; sodium-potassium-exchanging ATPase; steroids; tight junctions; transcription factors; transcriptional activation
Abstract:
... The interaction of ouabain with the sodium pump induces signalling cascades resembling those triggered by hormone/receptor interactions. In the rat Sertoli cell line 93RS2, ouabain at low concentrations stimulates the c-Src/c-Raf/Erk1/2 signalling cascade via its interaction with the α4 isoform of the sodium pump expressed in these cells, leading to the activation of the transcription factor CREB. ...
DOI:
10.1016/j.mce.2015.02.004

2. FOXO1 is regulated by insulin and IGF1 in pituitary gonadotropes

Author:
Skarra, Danalea V.; Thackray, Varykina G.
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 14-24
ISSN:
0303-7207
Subject:
apoptosis; cell proliferation; cytoplasm; insulin; insulin-like growth factor I; metabolism; phosphorylation; signal transduction; transcription factors
Abstract:
... The FOXO1 transcription factor is important for multiple aspects of reproductive function. We previously reported that FOXO1 functions as a repressor of gonadotropin hormone synthesis, but how FOXO1 is regulated in pituitary gonadotropes is unknown. The growth factors, insulin and insulin-like growth factor I (IGF1), function as key regulators of cell proliferation, metabolism and apoptosis in mul ...
DOI:
10.1016/j.mce.2015.02.006
PubMed:
25676570
PubMed Central:
PMC4363278

3. Glycated albumin triggers fibrosis and apoptosis via an NADPH oxidase/Nox4-MAPK pathway-dependent mechanism in renal proximal tubular cells

Author:
Qi, Weiwei; Niu, Jianying; Qin, Qiaojing; Qiao, Zhongdong; Gu, Yong
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 74-83
ISSN:
0303-7207
Subject:
glycation; reactive oxygen species; signal transduction; phosphorylation; mitogen-activated protein kinase; small interfering RNA; fibrosis; transforming growth factor beta; patients; hyperglycemia; fibronectins; apoptosis; caspase-3; NAD(P)H oxidase (H2O2-forming); messenger RNA; albumins; diabetic nephropathy; acetylcysteine; in vitro studies; kidneys; quantitative polymerase chain reaction; disease course
Abstract:
... Glycated albumin (GA), an Amadori product used as a marker of hyperglycemia and the early-stage glycation products compared to AGEs, might further promote kidney lesions in diabetic nephropathy (DN). However, the mechanisms how GA cause proximal tubular cells damage remain poorly understood. In this study, we investigated the effects of GA on fibrosis and apoptosis of renal proximal tubular cells ...
DOI:
10.1016/j.mce.2015.02.007

4. Paracrine communication modulates production of Wnt antagonists and COX1-mediated prostaglandins in a decidual-trophoblast co-culture model

Author:
Nayeem, Sarmah B.; Dharmarajan, Arun; Keelan, Jeffrey A.
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 52-62
ISSN:
0303-7207
Subject:
antagonists; coculture; gene expression; gene expression regulation; genes; messenger RNA; models; polymerase chain reaction; prostaglandins; proteins; secretion; trophoblast
Abstract:
... Wnt signalling has important roles in decidualisation, implantation and placentation. We investigated the role of decidua-trophoblast communication and Wnt signalling in the placenta using a co-culture model. Expression of a wide range of Wnt-related genes was observed in both decidual and trophoblast cells using PCR array, with remarkably similar expression profiles. Co-culture induced altered ex ...
DOI:
10.1016/j.mce.2015.02.003

5. PRDM16 sustains white fat gene expression profile in human adipocytes in direct relation with insulin action

Author:
Moreno-Navarrete, José María; Ortega, Francisco; Moreno, María; Xifra, Gemma; Ricart, Wifredo; Fernández-Real, José Manuel
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 84-93
ISSN:
0303-7207
Subject:
adipocytes; adipogenesis; gene expression; genes; homeostasis; humans; insulin; insulin resistance; messenger RNA; metformin; mitochondria; obesity; white adipose tissue
Abstract:
... In the present study, we aimed to evaluate the possible role of PRDM16 in human adipocytes and in whole adipose tissue according to obesity and insulin sensitivity. PRDM16 knockdown (KD) had a dual behavior. While KD in preadipocytes led to enhanced gene expression markers of adipocyte differentiation, PRDM16 KD in fully differentiated adipocytes resulted in decreased adipogenic gene expression an ...
DOI:
10.1016/j.mce.2015.01.042

6. Production, purification, and characterization of recombinant hFSH glycoforms for functional studies

Author:
Butnev, Viktor Y.; Butnev, Vladimir Y.; May, Jeffrey V.; Shuai, Bin; Tran, Patrick; White, William K.; Brown, Alan; Smalter Hall, Aaron; Harvey, David J.; Bousfield, George R.
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 42-51
ISSN:
0303-7207
Subject:
Western blotting; binding sites; bioactive properties; cell culture; culture media; electrophoresis; females; follicle-stimulating hormone; glycosylation; humans; hydrophobicity; immunoaffinity chromatography; molecular weight
Abstract:
... Previously, our laboratory demonstrated the existence of a β-subunit glycosylation-deficient human FSH glycoform, hFSH21. A third variant, hFSH18, has recently been detected in FSH glycoforms isolated from purified pituitary hLH preparations. Human FSH21 abundance in individual female pituitaries progressively decreased with increasing age. Hypo-glycosylated glycoform preparations are significantl ...
DOI:
10.1016/j.mce.2015.01.026
PubMed:
25661536
PubMed Central:
PMC4378652

7. Rac limits TGF-β-induced VEGF synthesis in osteoblasts

Author:
Yamamoto, Naohiro; Otsuka, Takanobu; Kondo, Akira; Matsushima-Nishiwaki, Rie; Kuroyanagi, Gen; Kozawa, Osamu; Tokuda, Haruhiko
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 35-41
ISSN:
0303-7207
Subject:
gene expression; guanosinetriphosphatase; messenger RNA; mitogen-activated protein kinase; mitogen-activated protein kinase kinase kinase; osteoblasts; phosphorylation; transforming growth factor beta; vascular endothelial growth factors
Abstract:
... We previously showed that transforming growth factor-β (TGF-β) stimulates vascular endothelial growth factor (VEGF) synthesis via p44/p42 mitogen-activated protein (MAP) kinase, p38 MAP kinase and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the involvement of Rac, which is a member of the Rho family of ...
DOI:
10.1016/j.mce.2015.02.002

8. The dipeptidyl peptidase-IV inhibitor inhibits the expression of vascular adhesion molecules and inflammatory cytokines in HUVECs via Akt- and AMPK-dependent mechanisms

Author:
Hwang, Hwan-Jin; Chung, Hye Soo; Jung, Tae Woo; Ryu, Ja Young; Hong, Ho Cheol; Seo, Ji A.; Kim, Sin Gon; Kim, Nan Hee; Choi, Kyung Mook; Choi, Dong Seop; Baik, Sei Hyun; Yoo, Hye Jin
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 25-34
ISSN:
0303-7207
Subject:
AMP-activated protein kinase; Western blotting; adhesion; anti-inflammatory activity; atherosclerosis; chemokine CCL2; dipeptidyl-peptidase IV; dose response; endothelium; enzyme inhibitors; foam cells; glycemic effect; human umbilical vein endothelial cells; interleukin-1beta; interleukin-6; lipopolysaccharides; low density lipoprotein; mitogen-activated protein kinase; phosphorylation; protective effect; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Recently, dipeptidyl peptidase-IV (DPP-IV) inhibitor, a major anti-hyperglycemic agent, has received substantial attention as a possible therapeutic target for inflammatory diseases such as atherosclerosis. However, the direct molecular mechanisms through which DPP-IV inhibitor mediates anti-inflammatory effects in vascular endothelial cells have not been clarified. The effects of the DPP-IV inhib ...
DOI:
10.1016/j.mce.2015.01.025

9. X-3, a mangiferin derivative, stimulates AMP-activated protein kinase and reduces hyperglycemia and obesity in db/db mice

Author:
Han, Jun; Yi, Jia; Liang, Fengying; Jiang, Bo; Xiao, Ying; Gao, Shouhong; Yang, Na; Hu, Honggang; Xie, Wei-Fen; Chen, Wansheng
Source:
Molecular and Cellular Endocrinology 2015 v.405 pp. 63-73
ISSN:
0303-7207
Subject:
AMP-activated protein kinase; acetyl-CoA carboxylase; acute toxicity; adipose tissue; blood glucose; death; glucose; hyperglycemia; hypothalamus; islets of Langerhans; liver; mechanism of action; metformin; mice; muscles; noninsulin-dependent diabetes mellitus; obesity; phosphorylation; toxicity testing; triacylglycerols
Abstract:
... Diabetes mellitus is a major health concern, affecting nearly 10% of the population. Here we describe a potential novel therapeutic agent for this disease, X-3, a derivative of mangiferin. Therapeutic administration of X-3 significantly and dose-dependently reduced plasma glucose and triglycerides in db/db mice following 8 week-treatments. Treatment with X-3 dose-dependently increased the number o ...
DOI:
10.1016/j.mce.2015.02.008

10. Advances in multifunctional glycosylated nanomaterials: preparation and applications in glycoscience

Author:
Adak, Avijit K.; Li, Ben-Yuan; Lin, Chun-Cheng
Source:
Carbohydrate research 2015 v.405 pp. 2-12
ISSN:
0008-6215
Subject:
bioassays; biosensors; carbohydrates; cell membranes; enzymes; glycosylation; image analysis; ligands; magnetism; metals; nanoparticles; vaccines
Abstract:
... Applications of glycosylated nanomaterials have gained considerable attention in recent years due to their unique structural properties and compatibility in biological systems. In this review, glyco-nanoparticles (glyco-NPs) are defined as compounds that contain a nano-sized metallic core, are composed of noble metals, magnetic elements, or binary inorganic nanoparticles, and that exhibit carbohyd ...
DOI:
10.1016/j.carres.2014.07.026

11. Assembling different antennas of the gp120 high mannose-type glycans on gold nanoparticles provides superior binding to the anti-HIV antibody 2G12 than the individual antennas

Author:
Chiodo, Fabrizio; Enríquez-Navas, Pedro M.; Angulo, Jesús; Marradi, Marco; Penadés, Soledad
Source:
Carbohydrate research 2015 v.405 pp. 102-109
ISSN:
0008-6215
Subject:
Human immunodeficiency virus; antiretroviral agents; biosensors; glycoproteins; gold; nanogold; neutralizing antibodies; polysaccharides; spectroscopy; surface plasmon resonance; synergism; vaccines; viral antibodies
Abstract:
... In order to re-build Man9GlcNAc2 clusters of the HIV gp120 glycoprotein, ∼2nm gold glyconanoparticles (GNPs) were coated with the synthetic partial structures of Man9, the tetramannoside Manα1–2Manα1–2Manα1–3Manα1– and the pentamannoside Manα1–2Manα1–3[Manα1–2Manα1–6]Manα1–. Their interactions with the anti-HIV broadly neutralizing antibody 2G12 were studied by surface plasmon resonance (SPR)-base ...
DOI:
10.1016/j.carres.2014.07.012

12. Carbohydrate conjugation through microwave-assisted functionalization of single-walled carbon nanotubes using perfluorophenyl azides

Author:
Kong, Na; Shimpi, Manishkumar R.; Park, Jae Hyeung; Ramström, Olof; Yan, Mingdi
Source:
Carbohydrate research 2015 v.405 pp. 33-38
ISSN:
0008-6215
Subject:
azides; carbohydrates; carbon nanotubes; chemical bonding; lectins; microwave radiation; microwave treatment
Abstract:
... Carbohydrate–functionalized single-walled carbon nanotubes (SWNTs) were synthesized using microwave-assisted reaction of perfluorophenyl azide with the nanotubes. The results showed that microwave radiation provides a rapid and effective means to covalently attach carbohydrates to SWNTs, producing carbohydrate–SWNT conjugates for biorecognition. The carbohydrate–functionalized SWNTs were furthermo ...
DOI:
10.1016/j.carres.2014.09.006

13. Electrochemical synthesis of nanostructured gold film for the study of carbohydrate–lectin interactions using localized surface plasmon resonance spectroscopy

Author:
Bhattarai, Jay K.; Sharma, Abeera; Fujikawa, Kohki; Demchenko, Alexei V.; Stine, Keith J.
Source:
Carbohydrate research 2015 v.405 pp. 55-65
ISSN:
0008-6215
Subject:
concanavalin A; dielectric spectroscopy; electrosynthesis; gold; image analysis; mannose; nanogold; potassium; refractive index; scanning electron microscopy; surface plasmon resonance
Abstract:
... Localized surface plasmon resonance (LSPR) spectroscopy is a label-free chemical and biological molecular sensing technique whose sensitivity depends upon development of nanostructured transducers. Herein, we report an electrodeposition method for fabricating nanostructured gold films (NGFs) that can be used as transducers in LSPR spectroscopy. The NGF was prepared by electrodepositing gold from p ...
DOI:
10.1016/j.carres.2014.08.019

14. Fluorous-based carbohydrate Quartz Crystal Microbalance

Author:
Chen, Lei; Sun, Pengfei; Chen, Guosong
Source:
Carbohydrate research 2015 v.405 pp. 66-69
ISSN:
0008-6215
Subject:
analytical chemistry; carbohydrates; catalytic activity; quartz crystal microbalance
Abstract:
... Fluorous chemistry has brought many applications from catalysis to separation science, from supramolecular materials to analytical chemistry. However, fluorous-based Quartz Crystal Microbalance (QCM) has not been reported so far. In the current paper, fluorous interaction has been firstly utilized in QCM, and carbohydrate–protein interaction and carbohydrate–carbohydrate interaction have been dete ...
DOI:
10.1016/j.carres.2014.07.023

15. Galactose grafting on poly(ε-caprolactone) substrates for tissue engineering: a preliminary study

Author:
Russo, Laura; Russo, Teresa; Battocchio, Chiara; Taraballi, Francesca; Gloria, Antonio; D’Amora, Ugo; De Santis, Roberto; Polzonetti, Giovanni; Nicotra, Francesco; Ambrosio, Luigi; Cipolla, Laura
Source:
Carbohydrate research 2015 v.405 pp. 39-46
ISSN:
0008-6215
Subject:
X-ray photoelectron spectroscopy; cell adhesion; cell viability; contact angle; epitopes; galactose; hardness; humans; hydrophilicity; lectins; mechanical properties; moieties; stem cells; tissue engineering
Abstract:
... The grafting of galactose units onto poly(ε-caprolactone) (PCL) substrates by a wet chemistry two-step procedure is proposed. Even though a reduction of hardness from 0.58–0.31GPa to 0.12–0.05GPa is achieved, the chemical functionalization does not negatively affect the tensile modulus (332.2±31.3MPa and 328.5±34.7MPa for unmodified and surface-modified PCL, respectively) and strength (15.1±1.3MPa ...
DOI:
10.1016/j.carres.2014.07.027

16. Heparin nanoparticles for β amyloid binding and mitigation of β amyloid associated cytotoxicity

Author:
Wang, Peng; Kouyoumdjian, Hovig; Zhu, David C.; Huang, Xuefei
Source:
Carbohydrate research 2015 v.405 pp. 110-114
ISSN:
0008-6215
Subject:
Alzheimer disease; amyloid; brain; cytotoxicity; enzyme-linked immunosorbent assay; gel electrophoresis; heparin; magnetism; nanoparticles; neurons
Abstract:
... Accumulation of β amyloid (Aβ) in the brain is believed to play a key role in the pathology of Alzheimer’s disease. Glycosaminoglycans on surface of neuronal cells can serve as nucleation sites to promote plaque formation on cell surface. To mimic this process, magnetic nanoparticles coated with heparin have been synthesized. The heparin nanoparticles were demonstrated to bind with Aβ through a va ...
DOI:
10.1016/j.carres.2014.07.020

17. Hyaluronic acid-modified multiwalled carbon nanotubes for targeted delivery of doxorubicin into cancer cells

Author:
Cao, Xueyan; Tao, Lei; Wen, Shihui; Hou, Wenxiu; Shi, Xiangyang
Source:
Carbohydrate research 2015 v.405 pp. 70-77
ISSN:
0008-6215
Subject:
biocompatibility; carbon nanotubes; cell viability; cellular microenvironment; chemical bonding; doxorubicin; drug carriers; drug therapy; fluorescein; hyaluronic acid; isothiocyanates; nanomedicine; neoplasm cells; neoplasms; pH; polyethyleneimine; receptors; viability assays; water solubility
Abstract:
... Development of novel drug carriers for targeted cancer therapy with high efficiency and specificity is of paramount importance and has been one of the major topics in current nanomedicine. Here we report a general approach to using multifunctional multiwalled carbon nanotubes (MWCNTs) as a platform to encapsulate an anticancer drug doxorubicin (DOX) for targeted cancer therapy. In this approach, p ...
DOI:
10.1016/j.carres.2014.06.030

18. Multivalent glycocyclopeptides: toward nano-sized glycostructures

Author:
Daskhan, Gour Chand; Berthet, Nathalie; Thomas, Baptiste; Fiore, Michele; Renaudet, Olivier
Source:
Carbohydrate research 2015 v.405 pp. 13-22
ISSN:
0008-6215
Subject:
cyclic peptides; glycoproteins; molecular weight; nanomaterials; synthesis
Abstract:
... Cyclopeptides have recently emerged as attractive molecular scaffolds for the multivalent presentation of carbohydrates in a well-defined constrained spatial orientation. This mini-review describes the last advances on the synthesis and the biological applications of these particular structures, going from low molecular weight glycoclusters to fully synthetic nano-sized glycodendrimers. ...
DOI:
10.1016/j.carres.2014.07.017

19. Polysaccharide-based nanocomposites and their applications

Author:
Zheng, Yingying; Monty, Jonathan; Linhardt, Robert J.
Source:
Carbohydrate research 2015 v.405 pp. 23-32
ISSN:
0008-6215
Subject:
nanocomposites; nanoparticles; polymers; polysaccharides
Abstract:
... Polysaccharide nanocomposites have become increasingly important materials over the past decade. Polysaccharides offer a green alternative to synthetic polymers in the preparation of soft nanomaterials. They have also been used in composites with hard nanomaterials, such as metal nanoparticles and carbon-based nanomaterials. This mini review describes methods for polysaccharide nanocomposite prepa ...
DOI:
10.1016/j.carres.2014.07.016

20. Sublingual injection of microparticles containing glycolipid ligands for NKT cells and subunit vaccines induces antibody responses in oral cavity

Author:
DeLyria, Elizabeth S.; Zhou, Dapeng; Lee, Jun Soo; Singh, Shailbala; Song, Wei; Li, Fenge; Sun, Qing; Lu, Hongzhou; Wu, Jinhui; Qiao, Qian; Hu, Yiqiao; Zhang, Guodong; Li, Chun; Sastry, K. Jagannadha; Shen, Haifa
Source:
Carbohydrate research 2015 v.405 pp. 87-92
ISSN:
0008-6215
Subject:
B-lymphocytes; T-lymphocytes; animal models; antibodies; antigen presentation; antigens; blood serum; cytokines; dendritic cells; glycolipids; hepatitis; immunogenicity; immunoglobulin A; immunoglobulin G; ligands; lymph nodes; macrophages; mice; microparticles; mouth; natural killer cells; neoplasms; ovalbumin; patients; saliva; secretion; subunit vaccines; toxicity
Abstract:
... Natural Killer T (NKT) cells are a unique type of innate immune cells which exert paradoxical roles in animal models through producing either Th1 or Th2 cytokines and activating dendritic cells. Alpha-galactosylceramide (αGalCer), a synthetic antigen for NKT cells, was found to be safe and immune stimulatory in cancer and hepatitis patients. We recently developed microparticle-formulated αGalCer, ...
DOI:
10.1016/j.carres.2014.11.007

21. Synthesis and cell-selective antitumor properties of amino acid conjugated tumor-associated carbohydrate antigen-coated gold nanoparticles

Author:
Biswas, Souvik; Medina, Scott H.; Barchi, Joseph J.
Source:
Carbohydrate research 2015 v.405 pp. 93-101
ISSN:
0008-6215
Subject:
alkanes; antigens; antineoplastic activity; binding proteins; carbohydrates; cell lines; galectins; nanogold; neoplasm cells; serine; therapeutics; threonine; vaccines
Abstract:
... The Thomsen Friedenreich antigen (TFag) disaccharide is a tumor-associated carbohydrate antigen (TACA) found primarily on carcinoma cells and rarely expressed in normal tissue. The TFag has been shown to interact with Galectin-3 (Gal-3), one in a family of β-galactoside binding proteins. Galectins have a variety of cellular functions, and Gal-3 has been shown to be the sole galectin with anti-apop ...
DOI:
10.1016/j.carres.2014.11.002

22. Synthesis and characterisation of glucose-functional glycopolymers and gold nanoparticles: study of their potential interactions with ovine red blood cells

Author:
Wilkins, Laura E.; Phillips, Daniel J.; Deller, Robert C.; Davies, Gemma-Louise; Gibson, Matthew I.
Source:
Carbohydrate research 2015 v.405 pp. 47-54
ISSN:
0008-6215
Subject:
agglutination; erythrocytes; glucose; glucose transporters; glycosylation; hemolysis; humans; image analysis; nanogold; polymerization; sheep
Abstract:
... Carbohydrate–protein interactions can assist with the targeting of polymer- and nano-delivery systems. However, some potential protein targets are not specific to a single cell type, resulting in reductions in their efficacy due to undesirable non-specific cellular interactions. The glucose transporter 1 (GLUT-1) is expressed to different extents on most cells in the vasculature, including human r ...
DOI:
10.1016/j.carres.2014.09.009

23. Synthesis and evaluation of glyco-coated liposomes as drug carriers for active targeting in drug delivery systems

Author:
Ueki, Akiharu; Un, Keita; Mino, Yuka; Yoshida, Mitsuru; Kawakami, Shigeru; Ando, Hiromune; Ishida, Hideharu; Yamashita, Fumiyoshi; Hashida, Mitsuru; Kiso, Makoto
Source:
Carbohydrate research 2015 v.405 pp. 78-86
ISSN:
0008-6215
Subject:
carbohydrate binding; carbohydrates; drug carriers; in vitro studies; receptors
Abstract:
... Novel sugar-conjugated cholesterols, β-Gal-, α-Man-, β-Man-, α-Fuc-, and β-Man-6P-S-β-Ala-Chol, were synthesized and incorporated into liposomes. In vitro experiments using the glyco-coated liposomes showed that the glyco-coated liposomes are efficiently taken up by cells expressing carbohydrate-binding receptors selectively. Glyco-coated liposomes are promising candidates for drug delivery vehicl ...
DOI:
10.1016/j.carres.2014.06.028