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... 24-Epibrassinolide (EBL) is considered the most probable brassinosteroid (BR) candidate that could be used for practical application in agriculture. EBL-induced stress-protective properties were evaluated in in vitro-grown tomato (Solanum lycopersicum L.) varieties Pusa Ruby (susceptible to nematodes) and PNR-7 (resistant to nematodes) during nematode pathogenesis. Sterilized tomato seeds treated ...
... To explore the RNA-dependent RNA polymerase (RdRP) function of the 3D protein of duck hepatitis A virus type 1 (DHAV-1), the gene was cloned into the pET-32a(+) vector for prokaryotic expression. The 3′ untranslated region (3′ UTR) of DHAV-1 together with a T7 promoter was cloned into the pMD19-T vector for in vitro transcription of 3′ UTR RNA, which was further used as a template in RNA-dependent ...
carbonates; chemical reactions; ethylene; ligands; sulfates
Abstract:
... Novel multimetallic copper pyrazolate complexes, inaccessible using simple pyrazole ligands due to competing, alternative structural motifs, can be obtained by locking pairs of pyrazole ligands together with ethylene tethers. Nanojars based on this tethered pyrazole ligand display unexpected total selectivity for the carbonate over the sulfate ion. ...
... Changes in the activity of Na⁺/K⁺-ATPase and content of membrane lipids (phospholipids) in the gills and hepatopancreas of the blue mussel Mytilus edulis L. have been studied during a sharp temperature increase under aquarian managed conditions. The most pronounced changes were recorded in mollusk gills. A correlation of changes in the activity of membrane-bound Na⁺/K⁺-ATPase and phospholipid cont ...
Dianthus chinensis; antioxidant activity; enzyme activity; ethylene; guard cells; hydrogen peroxide; hyperhydricity; in vitro culture; in vitro studies; micropropagation; plantlets; silver nitrate; water content
Abstract:
... Hyperhydricity symptoms are common and significant during the in vitro culture of Dianthus chinensis L. and greatly affect the micropropagation and regeneration of cultured plantlets. However, effective measures for preventing such abnormalities have not been developed for this species. Silver nitrate (AgNO₃) has been shown to revert hyperhydric plantlets to a normal state. Nevertheless, the effec ...
Cephalotaxus; Fourier transform infrared spectroscopy; alkaloids; electrospray ionization mass spectrometry; isomers; nuclear magnetic resonance spectroscopy; Vietnam
Abstract:
... Eight alkaloidal constituents have been isolated and structurally elucidated from Cephalotaxus mannii Hook. f. collected from the highland Lam Dong Province, Vietnam. They are: cephalotaxine (1), isoharringtonine (2), norisoharringtonine (3), desoxyharringtonine (4), nordesoxyharringtonine (5), cephalotaxine 𝛽 -N-oxide (6), 3-epi-schellhammericine (7), and an isomer of 3-epi-schellhammericine (8) ...
borates; chemical reactions; nuclear magnetic resonance spectroscopy
Abstract:
... Allyl borates, a new class of monofunctional polyhomologation initiators, are reported. These monofunctional initiators are less sensitive and more effective towards polymethylene-based architectures. As an example, the synthesis of α-vinyl-ω-hydroxypolymethylenes is given. By designing/synthesizing different allylic borate initiators, and using ¹H and ¹¹B NMR spectroscopy, the initiation mechanis ...
Rafael R. Cantú; Celso Aita; Alexandre Doneda; Diego A. Giacomini; Alexandre Dessbesell; Marlon Arenhardt; Géssica G. De Bastiani; Stefen B. Pujol; Philippe Rochette; Martin H. Chantigny; Sandro J. Giacomini
... Alternative fertilization practices are needed for reducing gaseous and leaching N losses at high urea application rates. The objective of this study was to compare gaseous N emissions (N₂O and NH₃) and NO₃ ⁻ concentrations in the soil solution during two successive lettuce cropping seasons under contrasting fertilization practices. Treatments were fertilization with regular urea (U), urea treated ...
V. G. Pshennikova; N. A. Barashkov; A. V. Solovyev; G. P. Romanov; E. E. Diakonov; N. N. Sazonov; I. V. Morozov; A. A. Bondar; O. L. Posukh; L. U. Dzhemileva; E. K. Khusnutdinova; M. I. Tomsky; S. A. Fedorova
... Тhe DNA testing of autosomal recessive deafness type 1A (DFNB1A, MIM 220290) is complicated when deaf patients have only monoallelic (heterozygous) recessive mutations in the GJB2 (Сх26) gene that is uninformative for establishment of diagnosis. Such patients may be “random” heterozygous carriers of GJB2 mutations as well as have the mutant allele in a cis-regulatory region of GJB2 gene, in elemen ...
... Biliary atresia (BA) is a rare and serious liver disease in newborn infants. Previously, we reported that non-parenchymal cell (NPC) fractions from cirrhotic liver of BA may contain hepatic stem/progenitor cells in primary culture of NPC fractions. In this study, NPC fractions were subjected to primary or passage culture and found that clusters of hepatocyte-like cells appear even without adding h ...
... Recent articles have provided estimates of evaporative flux from water tables in deserts that span 5 orders of magnitude. In this paper, we present an analytical calculation that indicates aquifer vapor flux to be limited to 0.01 mm/yr for sites where there is negligible recharge and the water table is well over 20 m below the surface. This value arises from the geothermal gradient, and therefore, ...
... The emergence and disappearance of antigenic variants of foot-and-mouth disease virus (FMDV) during a field outbreak occurs periodically due to the volatile nature of its genome. In the present analysis, change in antigenic behavior of serotype O FMDV during the serial cytolytic passage in the absence of immune pressure was observed. Initially, the isolate showed a poor antigenic match (relationsh ...
... The ability of alkalophilic micromycetes of the Emericellopsis alkalinа to produce antimicrobial peptides has been studied. Evaluation of the spectrum and the yield of antibiotic compounds has allowed us to choose a promising producer of peptide antimycotics, Emericellopsis аlkalinа А118. The producer exhibits antifungal activity against conditionally pathogenic yeast and mold fungi, i.e., Candida ...
DNA damage; antioxidant activity; apigenin; apoptosis; cytotoxicity; diabetes; enzyme activity; enzymes; free radical scavengers; in vitro studies; islets of Langerhans; lipid peroxidation; oxidative stress; protective effect; protein synthesis; rats; reactive oxygen species; streptozotocin; therapeutics; transcription (genetics)
Abstract:
... Pancreatic beta cells are very sensitive to oxidative stress, which is one of the major causes of cell damages in diabetes. Growing interest has focused on the development of effective therapeutics to protect pancreatic cells from oxidative stress and searching for potentially protective antioxidants for treating diabetes. Apigenin, a plant-derived flavonoid, was investigated to determine whether ...
... On behalf of the journal, the American Geophysical Union, and the scientific community, the editors would like to sincerely thank those who reviewed manuscripts for Water Resources Research in 2016. Their time spent reading and commenting on manuscripts not only improves the manuscripts themselves but also increases the scientific rigor of future research in the field. Many of those listed below w ...
... Somaclonal variation refers to the genetic and epigenetic changes in plants regenerated from plant tissue culture. In this study, using intersimple sequence repeat (ISSR) molecular markers, the somaclonal variation during micropropagation of sugarcane using temporary immersion bioreactors (TIBs) was evaluated. Apices of the cultivar Mex 69-290 were established and multiplied by ten subcultures in ...
... The association of polymorphism T-786C of the endothelial NO-synthase gene NOS3 with the functional state of the left ventricle (LV) was studied among residents of the West Siberian region with ischemic heart disease (IHD), including that combined with diabetes mellitus type 2 (DMT2). Carriers of genotype–786TT had the greatest ejection fraction of the left ventricle in the group of patients witho ...
Burkholderiaceae; Micrococcaceae; Streptomycetaceae; Xanthomonadaceae; application rate; bacterial communities; clay; community structure; crop yield; equations; granite; manure spreading; mineral fertilizers; phosphorus; sandstone; soil bacteria; soil pH; soil productivity; China
Abstract:
... A plot experiment was conducted to understand the response of the soil bacterial community to manure application rates and the relationship between the composition of bacterial community and soil chemical properties. The experiment involved gradients of manure combined with chemical fertilizer in red soils from granite, red sandstone and red clay between 2013 and 2015. The soil bacterial community ...
... River networks that drain mountain landscapes alternate between narrow and wide valley segments. Within the wide segments, beaver activity can facilitate the development and maintenance of complex, multithread planform. Because the narrow segments have limited ability to retain water, carbon, and nutrients, the wide, multithread segments are likely important locations of retention. We evaluated hy ...
Daniela Gabriel; Stefano G.A. Draisma; William E. Schmidt; Tom Schils; Thomas Sauvage; Clio Maridakis; C. Frederico D. Gurgel; D. James Harris; Suzanne Fredericq
Gibsmithia; algae; genetic markers; new species; phylogeny; Egypt; Federated States of Micronesia; Hawaii; Indonesia; Philippines; Red Sea; Saudi Arabia
Abstract:
... The tropical alga previously recognized as Gibsmithia hawaiiensis (Dumontiaceae, Rhodophyta) was recently suggested to represent a complex of species distributed throughout the Indo‐Pacific Ocean and characterized by a peculiar combination of hairy (pilose) gelatinous lobes growing on cartilaginous stalks. Phylogenetic reconstructions based on three genetic markers are presented here with the incl ...
antioxidant activity; bioactive compounds; chemical reactions; coordination compounds; films (materials); food industry; luteolin; myricetin; quercetin; water solubility
Abstract:
... We assembled dietary, bioactive flavonoids into a metal coordinated network to form thin, surface-bound films and hollow capsules, overcoming the poor water solubility of free flavonoids. Films formed from quercetin, myricetin, luteolin and fisetin show radical scavenging activity, a renowned feature of their parent flavonoids, and can be reused over multiple cycles. These films are expected to ha ...
Fourier transform infrared spectroscopy; Marsilea quadrifolia; X-ray diffraction; adipocytes; cytotoxicity; dose response; energy-dispersive X-ray analysis; glucose; in vitro studies; insulin; metformin; nanogold; scanning electron microscopy; screening; transmission electron microscopy; viability
Abstract:
... This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morpholog ...
... Increased loading of nitrogen (N) and phosphorus (P) from agricultural and urban intensification has led to severe degradation of inland and coastal waters. Lakes, reservoirs, and wetlands (lentic systems) retain these nutrients, thus regulating their delivery to downstream waters. While the processes controlling N and P retention are relatively well‐known, there is a lack of quantitative understa ...
... Azospirillum lipoferum Sp59b microbial cells were immobilized on the surface of thin polystyrene films modified in plasma from a high-frequency discharge of argon (13.56 MHz). The optimal conditions for immobilization, under which cell activity was maintained with respect to specific bacteriophages, were established. It was shown that it is possible to record the interaction of immobilized microbi ...
catalysts; catalytic activity; chemical reactions; dibenzofuran; ligands; phenanthrenes
Abstract:
... Bis(amidinate) ligands attached to rigid dibenzofuran and phenanthrene backbones have been introduced into lanthanide chemistry for the first time. Depending on the ligand and on the lanthanide source, either bi- and tetra-metallic macrocycles or monometallic complexes were obtained. The mono- and bi-metallic systems were compared in terms of their reactivity in the intramolecular hydroamination r ...
... The spatial variations in the elemental and stable carbon, nitrogen, and sulphur isotope composition of bladder wrack (Fucus vesiculosus) growing along the shore line of the semi-enclosed urbanized Kiel Fjord (western Baltic Sea) was investigated at more than 60 sites. The analyses of the carbon-nitrogen-sulphur (CNS) stoichiometry and C and N stable isotope signature of F. vesiculosus displayed s ...
acoustics; research; rivers; suspended sediment; water; British Columbia
Abstract:
... The use of “off‐the‐shelf” acoustic Doppler velocity profilers (ADCPs) to estimate suspended sediment concentration and grain‐size in rivers requires robust methods to estimate sound attenuation by suspended sediment. Theoretical estimates of sediment attenuation require a priori knowledge of the concentration and grain‐size distribution (GSD), making the method impractical to apply in routine mon ...
... On the Qinghai–Tibetan Plateau, isotopic signatures in soil–atmosphere CH ₄ fluxes were investigated in nine grasslands and three wetlands. In the grasslands, the fractionation factor for soil CH ₄ uptake, α ₛₒᵢₗ, was much smaller than the usually reported value of 0.9975–1.0095. Stepwise multiple variation analysis indicates that α ₛₒᵢₗ is higher for higher soil water contents but is lower for hi ...
... Recently, utilizing CO₂ as a methylation reagent to construct functional chemicals has attracted significant attention. However, the conversion of CO₂ is still a challenge due to its inherent inertness. In this study, we have developed a catalyst-free N-methylation of amines to prepare numerous methylamines using CO₂ as a methyl source. By utilizing 2 eq. PhSiH₃ as the reductant, amines could unde ...
... A series of crown-jewel Pd-based bimetallic nanostructures with tunable composition are fabricated inside the pores of an MOF via a hydride-induced-reduction strategy, exhibiting high activity and stability in the hydrogenation of nitrobenzene. ...
... Approximately half of the Padina (Dictyotales, Phaeophyceae) species mineralize aragonite needles over the adaxial thallus surface, where mineral bands are interspersed with nonmineralized regions along the thallus from the apical to basal end. However, this calcification pattern and the related algal properties are not well understood. Therefore, this work was performed to elucidate a potential r ...
Vaccinia virus; binding capacity; genes; mass spectrometry; proteins
Abstract:
... Identification of viral-host interacting proteins will contribute to understanding of how poxvirus exploits the host cellular machinery. The vaccinia virus gene K7R encodes a conserved protein K7 in most orthopoxviruses. To gain insight into the biology of K7, we investigated the cellular interactome of K7 by GST pulldown coupled with mass spectrometry. The top categories of identified proteins co ...
Whites; databases; frequency distribution; gene frequency; genome; genotype; loci; risk; single nucleotide polymorphism; stroke
Abstract:
... With the aim to determine specific genetic characteristics of the population of Moscow region, we determined allele frequencies of 48 polymorphic loci (SNP) associated with the increased risk of ischemic stroke development. The genotype frequency distribution for all the SNPs corresponds to Hardy–Weinberg equilibrium. Comparison of the allele frequencies with those obtained for Caucasian populatio ...
... The process of leaching of copper-zinc concentrate with a solution containing biogenic iron, which is a product of the metabolism of iron-oxidizing microorganisms, was studied. The dependence of leaching rate of metals on temperature and pH was determined. It was shown that up to 98% of zinc and 70% of iron could be removed from the concentrate, while up to 7 and 4 g/L of zinc and copper, respecti ...
advection; bitumen; byproducts; dissolved chlorides; geochemistry; lakes; mass transfer; oils; research; water
Abstract:
... Fluid fine tailings (FFT) are a principal by‐product of the bitumen extraction process at oil sands mines. Base Mine Lake (BML)—the first full‐scale demonstration oil sands end pit lake (EPL)—contains approximately 1.9 × 10⁸ m³ of FFT stored under a water cover within a decommissioned mine pit. Chemical mass transfer from the FFT to the water cover can occur via two key processes: (1) advection‐di ...
... Landouzy–Dejerine facioscapulohumeral muscular dystrophy (FSHD) is one of the most common hereditary myodystrophies. A study of the genetic nature of the disease, which has an autosomal dominant mode of inheritance, is extremely interesting and revealing. A unique structure of D4Z4 macrosatellite repeats found in the 4q35 region was originally characterized by a decrease in the number of repeats i ...
... Using collars for measuring soil respiration and its component fluxes in closed chamber systems relies on two main assumptions. Firstly, it is assumed that shallow collars prevent lateral soil gas leakage beneath the chamber’s walls and the underestimation of soil CO₂ fluxes, and secondly, the insertion of deeper collars excises all living roots and the autotrophic flux is eliminated. It was hypot ...
DNA; DNA barcoding; Phenacoccus solenopsis; biological control; biological control agents; cotton; genes; haplotypes; insects; mitochondria; nucleotide sequences; parasitoids; France; Pakistan
Abstract:
... Because correct identification of insects is crucial for pest management involving chemical or biological control agents, we have used a molecular approach to identify and characterize specimens of the cotton pest Phenacoccus solenopsis Tinsley (Sternorrhyncha: Pseudococcidae) present in different regions of Pakistan. The specimens were analyzed through DNA sequence analysis of their mitochondrial ...
... One natural recombinant subgroup B avian leukosis virus (ALV) with a subgroup J-like U3 region was isolated from commercial native chickens that experienced disease in 2014 and named GX14FF03. GX14FF03 was isolated by DF-1 cell culture and then identified with ELISA detection of avian leukosis virus p27 group-specific antigen, the detection of subtype specific PCR, and indirect immunofluorescence ...
air; carbon dioxide; rural areas; spectroscopy; stable isotopes; typhoons; urban areas; water vapor; Japan
Abstract:
... Isotope ratios of carbon dioxide and water vapour in the near-surface air were continuously measured for one month in an urban area of the city of Nagoya in central Japan in September 2010 using laser spectroscopic techniques. During the passages of a typhoon and a stationary front in the observation period, remarkable changes in the isotope ratios of CO ₂ and water vapour were observed. The isoto ...
Lewis acids; ammonium salts; bromides; catalysts; catalytic activity; chemical reactions; enantioselectivity; esters
Abstract:
... Epoxide desymmetrizations by bromide are very rare despite the large synthetic potential of chiral bromohydrins. Herein we present a new concept for epoxide desymmetrizations in which a bifunctional Lewis acid/ammonium salt catalyst allows for efficient enantioselective epoxide ring openings by Br⁻. With acetylbromide as a Br⁻ source bromohydrin esters are formed. ...
... Herein is reported the mild and general coupling of amine/ether C(sp³)–H bonds with various kinds of C(sp²)–O electrophiles with high selectivity and efficiency. Valuable allylic/benzylic amines are generated in moderate to excellent yields. The utility of this transformation is demonstrated by a broad substrate scope (>50 examples), good functional group tolerance and facile product modification. ...
alpha-tocopherol; chemical bonding; mass spectrometry; nuclear magnetic resonance spectroscopy; water solubility
Abstract:
... Covalent, ionic, and ionic-covalent conjugates of trolox and α-tocopherol with 1-aminoadamantane were synthesized. Their structures were elucidated using mass spectrometry and IR, PMR, and ¹³C NMR spectroscopy. The water solubility of the ionic trolox conjugates with 1-aminoadamantane increased by 2–24 times as compared with that of trolox whereas that of the α-tocopherol succinate conjugates rema ...
... The study of the poultry needs in basic nutrients allowed the development of a scheme for obtaining feed polypeptides (“polypeptide cassettes”) enriched with L-amino acids, which are necessary for the metabolism of birds. The amino acid and nucleotide profiles of about 500 bioinformation sequences of thermostable plant proteins and archaea were studied, on the basis of which candidate sequences we ...
... Microalgae cultivation for biofuels production and other applications has gained considerable interest recently. Despite their simple structures, microalgae can accumulate significant amounts of neutral lipids per dry cell weight compared to other energy crops. Neochloris oleoabundans is a promising microalga known for its high lipid content and biomass growth rate compared to other species cultiv ...
Dinophyceae; chloroplasts; estuaries; hairs; microtubules; nucleotide sequences; peduncle; phylogeny; ribosomal DNA; scanning electron microscopy; transmission electron microscopy
Abstract:
... A new marine woloszynskioid dinoflagellate Dactylodinium pterobelotum gen. et sp. nov., collected from a southern Vietnamese estuary, was described on the basis of LM, SEM, and TEM, and molecular phylogeny inferred from rDNA sequences. This species had the smallest number of amphiesmal vesicles (5 latitudinal series) in woloszynskioid dinoflagellates assigned to the Suessiaceae and Borghiellaceae. ...
Culicoides; adults; females; males; new species; pupae; scientific illustration; taxon descriptions; taxonomic keys; France; India
Abstract:
... Pupa, and adult male and female of Culicoides pseudosimilis n. sp., and pupae of two known species, C. selangorensis Wirth & Hubert, and C. dryadeus Wirth & Hubert, are described and illustrated. An identification key to the Indian species of Clavipalpis and Shermani species group of male Culicoides is also provided. ...
... We describe a novel species of torque teno mini virus called TTMV-204, which was isolated from the gingival epithelium of patients with periodontitis and characterized using viral metagenomics. The sequence of the full genome is 2824 nt in length. Phylogenetic analysis and genetic analyses show classic Betatorquevirus species organization with less than 40% amino acid similarity in ORF1. The preva ...
Markov chain; Monte Carlo method; fluorescein; hyporheic zone; models; research; solutes; sorption; streams; system optimization; water
Abstract:
... The complexity of hyporheic flow paths requires reach‐scale models of solute transport in streams that are flexible in their representation of the hyporheic passage. We use a model that couples advective‐dispersive in‐stream transport to hyporheic exchange with a shape‐free distribution of hyporheic travel times. The model also accounts for two‐site sorption and transformation of reactive solutes. ...
Markov chain; hydrologic data; prediction; research; sample size; water
Abstract:
... In this study, a copula‐based particle filter (CopPF) approach was developed for sequential hydrological data assimilation by considering parameter correlation structures. In CopPF, multivariate copulas are proposed to reflect parameter interdependence before the resampling procedure with new particles then being sampled from the obtained copulas. Such a process can overcome both particle degenera ...
energy balance; enthalpy; equations; freeze-thaw cycles; frozen soils; models; research; snow; snowpack; soil temperature; soil water; uncertainty; winter; China
Abstract:
... Snow and frozen soil are important factors that influence terrestrial water and energy balances through snowpack accumulation and melt and soil freeze‐thaw. In this study, a new land surface model (LSM) with coupled snow and frozen soil physics was developed based on a hydrologically improved LSM (HydroSiB2). First, an energy‐balance‐based three‐layer snow model was incorporated into HydroSiB2 (he ...
astrocytes; chemical reactions; fluorescent dyes; monitoring; sulfur; xanthenes
Abstract:
... We report a reversible off/on fluorescent probe for monitoring concentration changes of sulfane sulfur by utilizing the unique ability of sulfane sulfur to bind reversibly to other sulfur atoms and the intramolecular spirocyclization reaction of xanthene dyes. It reversibly visualized sulfane sulfur in living A549 cells and primary-cultured hippocampal astrocytes. ...
... The underwater light field in blackwater environments is strongly skewed toward the red end of the electromagnetic spectrum due to blue light absorption by colored dissolved organic matter (CDOM). Exposure of phytoplankton to full spectrum irradiance occurs only when cells are mixed up to the surface. We studied the potential effects of mixing‐induced changes in spectral irradiance on photoacclima ...
... Most of the solar-driven thermochemical CO₂ dissociation reactions have been focused on two-step processes. In this study, a one step CO₂ thermolysis process was considered. It was found that direct thermolysis of CO₂ obviously occurred at temperatures as low as 1200 °C within a corundum tube. The reaction rate could be enhanced by several times in the presence of metal oxides, which may be attrib ...
... We report a chemiresistive sensor approach based on a TTF–TCNQ charge transfer material, which can real-time detect and distinguish the vapors of alkyl amine and aromatic amine species under ambient conditions, based on the dramatic difference in the kinetics of the electric current recovery processes after the exposure of the two amine species. ...
... The aggregation-induced emission (AIE) effect has recently been widely applied for biomarker sensing. But developing “smart” strategies to effectively aggregate the AIE fluorogen and additionally enhance the fluorescence emission remain challenging. In this work, by integrating a biocompatible condensation reaction with an AIE fluorogen, we rationally designed a “smart” dual AIE probe Ac-Arg-Val-A ...
Bayesian theory; case studies; hysteresis; research; sediment contamination; sediment yield; sediments; stream flow; streams; time series analysis; turbidity; water; water quality; water supply; watersheds; New York
Abstract:
... This study presents a new method to examine long‐term dynamics in sediment yield using time‐varying sediment‐discharge rating curves. Dynamic linear models (DLMs) are introduced as a time series filter that can assess how the relationship between streamflow and sediment concentration or load changes over time in response to a wide variety of natural and anthropogenic watershed disturbances or long ...
... The effect of potassium sorbate, sodium benzoate, and sodium nitrite used as preservatives in the food industry in the production of such mytotoxins as citrinin cyclopiazonic and mycophenolic acids by the contaminating fungi Penicillium citrinum, P. commune, and P. brevicompactum, respectively, was investigated. It was shown that the effect of preservatives used at concentrations relevant to the f ...
hydraulic flumes; models; research; rivers; streams; surveys; topography; water
Abstract:
... We focus on the measurement of the bed‐elevation of braided networks in flume experiments. In particular, the effect of the survey frequency on the measurement accuracy is studied. To this aim, an innovative measurement system is adopted. It consists of a laser‐ultrasonic sensor and can survey the bed‐elevation under flowing water. This measurement system was used to profile a flume transect with ...
... We report the detection of rabbit haemorrhagic disease virus 2 (RHDV2) in the Madeira archipelago, Portugal. Viral circulation was confirmed by RT-qPCR and vp60 sequencing. Epidemiological data revealed the outbreak initiated in October 2016 in Porto Santo affecting wild and domestic rabbits. It was then detected three months later on the island of Madeira. Five haplotypes were identified and a ge ...
... The widely used plastic film containing di(2-ethylhexyl) phthalate (DEHP) in agriculture has caused serious soil pollution and poses risks to human health through the food chain. An effective DEHP degradation bacteria, Microbacterium sp. J-1, was newly isolated from landfill soil. Response surface methodology was successfully employed for optimization resulting in 96% degradation of DEHP (200 mg L ...
absorption; chemical compounds; chemical reactions
Abstract:
... Dissymmetrical substitution of norcorrole enables the preparation of various norcorrole derivatives as air- and moisture-stable species. Intramolecular charge transfer interactions between electron-donating substituents and the norcorrole core in these norcorroles significantly enhanced their low-energy absorption bands, which were forbidden in symmetrical Niᴵᴵ dimesitylnorcorrole. ...
Solanum betaceum; aspartic proteinases; callus; cell suspension culture; fluorometry; glycolysis; growth models; in vitro studies; liquids; mass spectrometry; metalloproteinases; protein composition; proteins; proteolysis; serine proteinases; somatic embryogenesis; xylanases
Abstract:
... Somatic embryogenesis (SE) is an important biotechnological tool with great potential for large-scale cloning. In Solanum betaceum Cav. (tamarillo), embryogenic (EC) and non-embryogenic (non-EC) cells can be obtained from the same explant on auxin-containing medium, making this system ideal for the evaluation of biochemical changes occurring during embryogenic induction. Liquid cultures offer addi ...
... Safed musli (Chlorophytum borivilianum Santapau & R.R.Fern.) is a medicinal herb valued for its tuberous roots that contain several bioactive compounds. Conventionally, safed musli tubers are harvested for secondary metabolites 9 mo after planting. Alternatively, in vitro culture can be used for mass production and to shorten the time required to produce secondary metabolites. This study was desig ...
... Primary mouse intestinal epithelial cells (MIEs) are not ideal models for long-term culture in vitro and a limited amount of approximate three generations. In addition, the mechanism that arginine-arginine dipeptide (Arg-Arg) regulates mouse intestinal inflammatory response remains unknown. Therefore, the aim of this study was to establish immortal MIEs and study the effects of Arg-Arg on inflamma ...
Soil and Water Assessment Tool model; drinking water; equations; model validation; monitoring; nitrate nitrogen; nitric oxide; nutrient content; nutrients; prediction; temperature; water supply; watersheds; wetlands; Gulf of Mexico
Abstract:
... The ability to accurately simulate flow and nutrient removal in treatment wetlands within an agricultural, watershed-scale model is needed to develop effective plans for meeting nutrient reduction goals associated with protection of drinking water supplies and reduction of the Gulf of Mexico hypoxic zone. The objectives of this study were to incorporate new equations for wetland hydrology and nutri ...
... Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (Danio rerio). Here we focus on mzfDB3, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized mzfDB3 gene with a 6×His-tag at the 3′-end was inserted into the pPICZ ...
Alternaria tenuissima; anion exchange; coumaric acids; culture filtrates; enzyme kinetics; feruloyl esterase; fungi; gel chromatography; lignocellulose; mass spectrometry; molecular weight; polyacrylamide gel electrophoresis; wood flour
Abstract:
... An extracellular feruloyl esterase from the culture filtrates of the isolated fungus Alternaria tenuissima was successfully purified to apparent homogeneity by anion-exchange and size-exclusion chromatography. Peptide fragments of purified enzyme (designated as AltFAE; molecular weight of 30.3 kDa determined by SDS-PAGE) were identified by mass spectrometry using a NanoLC-ESI-MS/MS system. Michael ...
... In the summer of 2015, a series of thermal tracer tests were conducted at the Widen field site in northeast Switzerland to validate travel time‐based thermal tracer tomography for reconstruction of aquifer heterogeneity. Repeated thermal tracer tests and distributed temperature observations were used to obtain a multisource/multireceiver tomographic experimental setup. After creating forced hydrau ...
alkaloids; amines; ammonia; antioxidant activity; free radicals; taxifolin; water solubility
Abstract:
... The reaction of dihydroquercetin monosuccinate with ammonia, a series of alkylamines, and amino-derivatives of heterocyclic compounds including nitroxyl radicals and alkaloids produced the corresponding ionic conjugates, the water solubility of which was 1–2 orders of magnitude greater than that of dihydroquercetin with retention of the high antioxidant activity. ...
... Sapoviruses (SaVs) are enteric viruses and have been detected in various mammals. They are divided into multiple genogroups and genotypes based on the entire major capsid protein (VP1) encoding region sequences. In this study, we determined the first complete genome sequences of two genogroup V, genotype 3 (GV.3) SaV strains detected from swine fecal samples, in combination with Illumina MiSeq seq ...
Kenneth Neil Mertens; Haifeng Gu; Vera Pospelova; Nicolas Chomérat; Elisabeth Nézan; Pieter Roger Gurdebeke; Kara Bogus; Henk Vrielinck; Myriam Rumebe; Claire Meteigner
Prorocentrum; beta-glucans; blood platelets; endospores; hydrofluoric acid; light microscopy; nucleotide sequences; palynology; plankton; ribosomal DNA; scanning electron microscopy; France
Abstract:
... The resting cysts of the benthic dinoflagellate Prorocentrum leve from a natural reservoir in Gujan‐Mestras (Gironde, France) were described in this study. The incubated urn‐shaped cysts gave rise to cells of P. leve. Morphological observations through light microscopy and scanning electron microscopy, particularly of the periflagellar platelets, combined with large subunit ribosomal DNA sequences ...
... Since 2014, cases of hemangioma associated with avian leukosis virus subgroup J (ALV-J) have been emerging in commercial chickens in Guangxi. In this study, four strains of the subgroup J avian leukosis virus (ALV-J), named GX14HG01, GX14HG04, GX14LT07, and GX14ZS14, were isolated from chickens with clinical hemangioma in 2014 by DF-1 cell culture and then identified with ELISA detection of ALV gr ...
air; chemical reactions; polymers; solar cells; solvents; xylene
Abstract:
... Progress towards practical organic solar cells amenable to large scale production is reported. Fullerene-free organic solar cells with a PCE of ∼4.8% are achieved based upon an active layer composed of the standard donor polymer PTB7-Th and a highly soluble twisted PDI acceptor tPDI-Hex. All devices can be fabricated and tested in air with ‘as-cast’ active layers being processed from the greener s ...
chemical bonding; chemical reactions; moieties; polymers
Abstract:
... Stable phospha- and arsaalkenes were used to synthesize polymers containing unsaturated PC and AsC moieties. The composition, chemical environment, structure, optical, and electronic properties of the monomers and polymers were elucidated. The incorporation of the heteroatom–carbon double bonded units efficiently perturbs the optoelectronics and solid state features of both monomeric and polymer ...
... We carried out an allozyme analysis to investigate polymorphism and genetic structure of the populations of D. incarnata and D. ochroleuca in regions of their joint growth in Russia and Belarus. We found that D. ochroleuca individuals in the populations of the Urals and Siberia, which are distant fragments from the main range of the species, do not differ significantly from individuals within the ...
... The intraspecific structure of the European anchovy (Engraulis encrasicolus) was studied on the basis of mitochondrial cytochrome b gene (cytb) fragment variability in 84 individuals from seven localities of the Black Sea and the Sea of Azov. The data on haplotype and nucleotide diversity and the values of neutrality tests suggested expansive growth of anchovy populations in the Azov-Black Sea bas ...
... Human bocavirus (HBoV) is a single-stranded DNA virus in Parvoviridae family, causing respiratory diseases in human. The recent identifications of genomic recombination among the four human bocavirus genotypes and related non-human primate bocaviruses have shed lights into the evolutionary processes underpinning the diversity of primate bocavirus. Among these reports, however, we found inconsisten ...
... To date, the efficacy of the phenylalanine hydroxylase (PAH) cofactor is proved for the treatment of both BH4-dependent hyperphenylalaninemia and phenylketonuria patients with mutations in the PAH gene. Since the patient’s response depends on the presence of residual PAH enzyme activity, it is advisable to search for mutations in the PAH gene to identify the potential responders and nonresponders ...
entropy; prediction; research; solutes; statistical analysis; variance; water
Abstract:
... We propose a novel approach to link solute transport behavior to the physical heterogeneity of the aquifer, which we fully characterize with two measurable parameters: the variance of the log K values (σY2), and a new indicator (HR) that integrates multiple properties of the K field into a global measure of spatial disorder or geological entropy. From the results of a detailed numerical experiment ...
... A gold-catalyzed sequential annulation reaction to prepare 3,4-fused bicyclic furan compounds has been realized by employing 2-(1-alkynyl)-2-alken-1-ones and 1,3,5-triazines as the starting materials under mild reaction conditions. This protocol features multiple bond formation in a single operation with the incorporation of two nitrogen and two carbon atoms into the final products. A mechanistic ...
... We evaluated the impact of exponential fertilization in nursery and weed removal in the field on growth and nitrogen (N) retranslocation and uptake from the soil of jack pine (Pinus banksiana Lamb.) seedlings planted on an oil sands reclaimed soil. Exponential fertilization is a method of supplying nutrients at an exponential rate to achieve constant internal nutrient concentrations in seedlings w ...
Hepatitis B virus; Retroviridae; chromosomes; hepatoma; human cell lines; humans; messenger RNA; transactivators; transcription factor NF-kappa B; trophoblast
Abstract:
... Human endogenous retrovirus W family (HERV-W) envelope (env) at chromosome 7 is highly expressed in the placenta and possesses fusogenic activity in trophoblast development. HERV-W env has been found to be overexpressed in some cancers and immune diseases. Viral transactivators can induce the overexpression of HERV-W env in human cell lines. Hepatitis B virus X protein (HBx) is believed to be a mu ...
household surveys; issues and policy; prices; research; water; water pollution; water shortages; China
Abstract:
... China continues to deal with severe levels of water scarcity and water pollution. To help address this situation, the Chinese central government initiated urban water pricing reforms in 2002 that emphasized the adoption of increasing block rate (IBR) price structures in place of existing uniform rate structures. By combining urban water use records with microlevel data from the Chinese Urban House ...
Herpesvirales; bioactive properties; gene transfer; hosts; immunomodulators; interleukin-10; major histocompatibility complex; natural killer cells; protein structure; proteins; virus replication
Abstract:
... Herpesviruses have acquired numerous genes from their hosts. Although these homologs are not essential for viral replication, they often have important immunomodulatory functions that ensure viral persistence in the host. Some of these viral molecules are called virokines as they mimic cellular cytokines of their host such as interleukin-10 (cIL-10). In recent years, many viral homologs of IL-10 ( ...
Bacillus subtilis; biofilm; electron microscopy; environmental factors; polymers; research; sediment transport; sediments; shear stress; water
Abstract:
... Extracellular polymeric substances (EPS) are ubiquitous on tidal flats but their impact on sediment erosion has not been fully understood. Laboratory‐controlled sediment beds were incubated with Bacillus subtilis for 5, 10, 16, and 22 days before the erosion experiments, to study the temporal and spatial variations in sediment stability caused by the bacterial secreted EPS. We found the biosedimen ...
... Our aim was to search for new cellular binding partners for the E6 and E7 oncogenes of beta human papillomaviruses (HPV), whose direct role in skin carcinogenesis has not been thoroughly investigated. By employing glutathione S-transferase pulldown and coimmunoprecipitation, we identified nuclear myosin 1c as a binding partner of HPV 8 E7 protein. As nuclear myosin 1c is an essential component of ...
... Compared with Li₄Ti₅O₁₂, LiCrTiO₄ with the same spinel structure exhibits superior conductivity and Li⁺ diffusion properties. However, there has been no extensive study on LiCrTiO₄ as an anode material in lithium ion batteries, due to its pulverization, loss of electrical contact, and particle aggregation. A unique architecture based on hybrid porous CNTs embedding ultrasmall LiCrTiO₄ nanoparticle ...
Arabidopsis thaliana; catalase; chlorophyll; enzyme activity; genes; genotype; growth retardation; hydrogen peroxide; leaves; peroxidase; proline; salicylic acid; salt stress; salt tolerance; signal transduction; sodium chloride; superoxide dismutase
Abstract:
... The effect of hydrogen peroxide treatment on the salt tolerance of wild-type Arabidopsis thaliana L. plants (Col-0) and plants transformed with the bacterial salicylate hydroxylase gene (NahG) was studied. The base tolerance to salt stress caused by 200 mM of NaCl in solution culture was higher in plants with the NahG genotype in comparison with the wild-type plants. Growth inhibition was observed ...
... Complex subsurface flow dynamics impact the storage, routing, and transport of water and solutes to streams in headwater catchments. Many of these hydrogeologic processes are indirectly reflected in observations of stream chemistry responses to rain events, also known as concentration‐discharge (CQ) relations. Identifying the relative importance of subsurface flows to stream CQ relationships is of ...
females; freezing; in vitro fertilization; in vitro studies; males; metaphase; mice; oocytes; semen; survival rate; vitrification
Abstract:
... Oocyte incubation time before freezing is one of the factors affecting oocyte vitrification. In the assisted reproductive technology (ART) clinics, it is sometimes decided to perform oocyte vitrification after a long period of incubation time due to various conditions, such as inability to collect semen samples, unsuccessful urological interventions (PESA, TESE, etc.), or unexpected conditions. A ...
cathodes; chemical reactions; ions; sodium; thermodynamics
Abstract:
... In operando powder X-ray diffraction data reveals an unexpected structural evolution in a layered P2 type NaₓCo₀.₇Mn₀.₃O₂ material during cycling. The population of single crystallographic sites sometimes increases even though sodium ions are being extracted from the structure, implying a highly cooperative diffusion mechanism. The structural evolution is shown to proceed through a series of metas ...
chemical bonding; pH; polymerization; polymers; temperature
Abstract:
... In situ fabrication of a temperature and pH dual-responsive linear supramolecular polymer through the marriage of dynamic covalent bonding and pillar[5]arene-based host–guest interaction was investigated. ...
... 9H-[1,2,4]Triazolo[4,3-g]purin-5-amine (2) was synthesized by reaction of 6-hydrazinyl-7H-purin-2-amine (1a) with formic acid under reflux conditions. Treating compound 1a in ethanol with CS₂ in the presence of KOH yielded 5-amino-3H-[1,2,4]triazolo[4,3-g]purine-3-thione (3). The new synthesized compounds were characterized using IR, ¹H NMR, and elemental analysis. The synthesized compounds have b ...
friction velocity; hypoxia; oxygen; oxygen consumption; research; sediments; systems engineering; turbulent flow; water; water management; water quality
Abstract:
... Hypolimnetic oxygenation is an increasingly common lake management strategy for mitigating hypoxia/anoxia and associated deleterious effects on water quality. A common effect of oxygenation is increased oxygen consumption in the hypolimnion and predicting the magnitude of this increase is the crux of effective oxygenation system design. Simultaneous measurements of sediment oxygen flux (JO₂) and t ...
Citrus; electrical resistance; freshwater; orchards; preferential flow; research; rhizosphere; soil heterogeneity; soil restoration; soil sampling; soil water content; spring; tomography; wastewater; wastewater irrigation; water content; water flow; water repellent soils; Israel
Abstract:
... The recognition of treated wastewater (TWW) as an alternative water resource is expanding in areas with a shortage of freshwater (FW) resources. Today, most orchards in Israel are irrigated with TWW. While the benefits of using TWW for irrigation are apparent, evidence of its negative effects on soil, trees, and yield is accumulating. This study, performed in a commercial TWW‐irrigated citrus orch ...
... An artificial neural network (ANN) was used to analyze photometric features extracted from the digitized images of leaves from in vitro-regenerated potato plants for non-invasive estimation of chlorophyll content. A MATLAB®-based, feed-forward, backpropagation-type network was developed for an input layer (three input elements), with one hidden layer (one node) and one output layer representing th ...
Youngjin JangY. J. and A. S. contributed equally to this work.; Arthur Shapiro; Maya Isarov; Anna Rubin-Brusilovski; Aron Safran; Adam K. Budniak; Faris Horani; Joanna Dehnel; Aldona Sashchiuk; Efrat Lifshitz
... Semiconductor colloidal quantum dots (CQDs) have attracted vast scientific and technological interest throughout the past three decades, due to the unique tuneability of their optoelectronic properties by variation of size and composition. However, the nanoscale size brings about a large surface-to-bulk volume ratio, where exterior surfaces have a pronounced influence on the chemical stability and ...
Green-Ampt equation; air; head; hydraulic conductivity; research; soil water characteristic; water content
Abstract:
... Analytical and numerical solutions for flow through partially saturated soils typically require functional relationships between water content, pressure, and hydraulic conductivity. Here we propose a Weibull‐type function to describe the hydraulic conductivity‐pressure head function. We show that this is a more flexible function that has the ability to address air entry pressure, while retaining t ...
... Polypeptide fractions from seeds of Fritillaria pallidiflora Schrenk were isolated and purified. The peptide compositions and molecular masses were determined using SDS-PAAG electrophoresis and LC-MS analysis. The results showed that the most active fraction of biocidal peptides contained greater than 20 constituents with molecular masses in the range of 1050–4727.2 Da. The isolated peptide fracti ...