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Pierre Billon; Jian Li; Jean-Philippe Lambert; Yizhang Chen; Véronique Tremblay; Joseph S. Brunzelle; Anne-Claude Gingras; Alain Verreault; Tomohiko Sugiyama; Jean-Francois Couture; Jacques Côté
DNA; DNA damage; DNA replication; acetylation; cohesion; crystal structure; genome; homologous recombination; lysine; mutation; post-translational modification; proliferating cell nuclear antigen; protein subunits
Abstract:
... During DNA replication, proliferating cell nuclear antigen (PCNA) adopts a ring-shaped structure to promote processive DNA synthesis, acting as a sliding clamp for polymerases. Known posttranslational modifications function at the outer surface of the PCNA ring to favor DNA damage bypass. Here, we demonstrate that acetylation of lysine residues at the inner surface of PCNA is induced by DNA lesion ...
... CASE HISTORY: A herd of Holstein, Jersey, or Holstein-Jersey cross lactating cattle of mixed ages presented with a sudden drop in milk yield in 94/678 cows on 3 October 2014 (Day 0). The herd was located in Gretna in the Derwent Valley (Tasmania, Australia) and had been grazing dryland pasture. CLINICAL FINDINGS: On Day 0 the cows variably showed recumbency, peracute photosensitisation, inflamed c ...
air pollutants; air pollution; air quality; carbon monoxide; economic development; income; issues and policy; monitoring; nitrogen dioxide; ozone; sulfur dioxide; Canada
Abstract:
... The environmental Kuznets curve hypothesis suggests that at high income levels, economic growth is accompanied by decreasing concentrations of air pollutants. We examine the relationship between four common air pollutants and income across Canadian provinces and metropolitan areas. Our study improves upon past studies of the relationship in Canada in two ways. First, our use of panel methods and p ...
... Antroquinonol (ANQ) is a ubiquinone derivative from the unique mushroom Antrodia camphorata, which exhibits broad-spectrum bioactivities. The effects of ANQ on cancer stem cell-like properties in colon cancer, however, remain unclear. In this study, we found that ANQ inhibited growth of colon cancer cells. The 50% growth inhibitions (GI₅₀) of ANQ on HCT15 and LoVo were 34.8 ± 0.07 and 17.9 ± 0.07 ...
... Arginine methylation is a common post-translational modification functioning as an epigenetic regulator of transcription and playing key roles in pre-mRNA splicing, DNA damage signaling, mRNA translation, cell signaling, and cell fate decision. Recently, a wealth of studies using transgenic mouse models and selective PRMT inhibitors helped define physiological roles for protein arginine methyltran ...
... The potential use of allelopathic and signaling compounds as environmentally friendly agrochemicals is a subject of increasing interest, but the fate of these compounds once they reach the soil environment is poorly understood. This work studied how the sorption, persistence, and leaching of the two enantiomers of the phytohormone abscisic acid (ABA) in agricultural soil was affected by the amendm ...
... Assessing habitat naturalness belongs to the most current issues in conservation biology. It has been recognized that plants are able to indicate the naturalness of their habitat. Thus, species may be given relative naturalness indicator values (i.e. scores on an ordinal scale), reflecting their different tolerances against habitat degradation. In the present study, our first goal was to test whet ...
Porcine reproductive and respiratory syndrome virus; antibodies; gel electrophoresis; genes; mice; nucleic acids; nucleocapsid; open reading frames; proteins; swine
Abstract:
... A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains — a type I and a type II — are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assay ...
DNA damage; bioavailability; cell viability; chemoprevention; colon; colorectal neoplasms; dietary fiber; digestion; flavorings; inhibitory concentration 50; intestinal microorganisms; phenolic acids; polyphenols; pomace; red wines; seeds; short chain fatty acids; small intestine
Abstract:
... This study evaluates the antiproliferative and antigenotoxic actions of powdered red wine pomace seasonings (Sk-S, seedless; W–S, whole; Sd-S, seeds). In vitro gastrointestinal digested and colonic fermented fractions of the seasonings were used as cell treatments. Phenolic acids from Sk-S showed the highest bioaccessibility in the small intestine, whereas polyphenols contained in Sd-S might be th ...
... There is a lack of data on comparative anatomy of the assimilatory organs of the enigmatic carnivorous Nepenthes species; the linkages between their leaf tissue anatomy and physico-chemical properties are also rarely considered. We examined the anatomy of the leaf (lamina) and its conjoint pitcher in five Nepenthes species (Nepenthes ampullaria, N. bicalcarata, N. gracilis, N. hemsleyana and N. ra ...
... AIM: To evaluate the level of agreement of three indirect testing methods with concentrations of IgG in serum, and to determine their test characteristics for diagnosing failure of passive transfer (FPT), in dairy calves in New Zealand. METHODS: From 17 July to 30 November 2015, 471 blood samples were collected from dairy calves aged 0–8 days, from 19 different commercial dairy farms in the Waikat ...
Myrtaceae; Nothofagus; canopy; forest succession; seedlings; wildfires; rain forests; species diversity; stand basal area; New Caledonia
Abstract:
... Mechanisms leading to monodominance in rainforests are still commonly discussed within the framework of forest succession. Here, we focused on the comparison of two monodominant species, Arillastrum gummiferum (Myrtaceae) and Nothofagus aequilateralis (Nothofagaceae), to try to better understand the underlying ecological mechanisms. Those two species are known to dominate the upper canopy of some ...
Moreno-Perez Sonia; Turati Daniela Flavia Machado; Borges Janaina Pires; Luna Pilar; Señorans Francisco Javier; Guisan Jose M.; Fernandez-Lorente Gloria
... Different immobilized derivatives of two lipases were tested as catalysts of the synthesis of ethyl esters of omega-3 fatty acids during the ethanolysis of sardine oil in solvent-free systems at 25 °C. Lipases from Thermomyces lanuginosus (TLL) and Lecitase Ultra (a phospholipase with lipolytic activity) were studied. Lipases were adsorbed on hydrophobic Sepabeads C18 through the open active cente ...
Coleoptera; bark; conservation areas; dead wood; forest ecosystems; forests; hardness; intensive forestry; logging; microhabitats; stumps; wood moisture; xylem; Central European region
Abstract:
... Saproxylic beetles are essential for the proper functioning of forest ecosystems. However, intensive forest management has led to the disappearance of suitable microhabitats of many species associated with dead wood. Cucujus cinnaberinus is an endangered saproxylic beetle which develops under the bark of dead wood. The species usually occurs in protected areas, however, some populations inhabit lo ...
Bacillariophyceae; correspondence analysis; environmental factors; exposure duration; fossils; least squares; prediction; salinity; salt marshes; sea level; Chile
Abstract:
... To reconstruct sea‐level history from changes in tidal environments using diatom assemblages, we need to better understand the relations among brackish diatom assemblages and changing environments along elevational gradients from diverse coastal sites. Our statistical analysis reveals relations between environmental variables and brackish benthic diatom assemblages in the little studied region of ...
... The influence of variable culture conditions on the size and wet density of spores of Bacillus cereus and Bacillus megaterium were examined in this work. Culture temperature and initial pH was shown to have a significant impact on the size of both species, with increasingly alkaline culture media and elevated culture temperatures resulting in spores that were, on average, up to 25% reduced in volu ...
Pinus sylvestris; air pollution; ecological value; environmental quality; epiphytes; eutrophication; fertilizer industry; lichens; nitrogen fertilizers; species diversity; thallus; Lithuania
Abstract:
... The objective of the study was to evaluate the impact of nitrogen fertilizer industry pollution on epiphytic lichen communities. The study plots are located in Scots pine Pinus sylvestris stands at different distances (up to 12 km) to the northeast and southwest of the nitrogen fertilizer producer plant in central Lithuania. The stands were semi-mature and mature and growing on sandy sites of the ...
... The substrate specificity of aminopeptidases has often been determined against aminoacyl-p-nitroanilide; thus, its specificity toward synthetic peptides and complex substrates remained unclear. The hydrolysis specificity of an aminopeptidase from Bacillus licheniformis SWJS33 (BLAM) was evaluated using a series of synthetic peptides and soybean protein isolate. The aminopeptidase showed high speci ...
... Carbonic anhydrase (CA), Na+/K+-ATPase (NKA) and Vacuolar-type H+-ATPase (HAT) play vital roles in osmoregulation and pH balance in decapod crustaceans. As variable pH levels have a significant impact on the physiology of crustaceans, it is crucial to understand the mechanisms by which an animal maintains its internal pH. We examined expression patterns of cytoplasmic (CAc) and membrane-associated ...
... CASE HISTORY: A 1-year-old female New Zealand sea lion (Phocarctos hookeri) was intermittently observed in the Otago region of New Zealand over an 11-month period, always dragging her hind flippers. In December 2012 the sea lion was found dead, after a period of several days being observed to be harassed by male sea lions. PATHOLOGICAL FINDINGS: At gross postmortem examination the sea lion was in ...