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1. CRTC2 suppresses BMP2-induced osteoblastic differentiation via Smurf1 expression in MC3T3-E1 cells

Author:
Kim, Kyeong-Min; Jeon, Wan-Jin; Kim, Eun-Jung; Jang, Won-Gu
Source:
Life sciences 2018 v.214 pp. 70-76
ISSN:
0024-3205
Subject:
Western blotting; alizarin; alkaline phosphatase; bone formation; cyclic AMP; extracellular matrix; gene overexpression; genes; gluconeogenesis; luciferase; messenger RNA; mineralization; mothers against decapentaplegic homolog proteins; osteoblasts; protein content; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; transcription (genetics); tumor necrosis factor-alpha
Abstract:
... CREB (cAMP response element-binding protein)-regulated transcription coactivator (CRTC2) has been reported to act as a coactivator of CREB during gluconeogenesis. The role of CRTC2 in osteoblastic differentiation has not yet been elucidated. The aim of this study is to identify the mechanism of CRTC2 in osteoblast differentiation.The mRNA expression was determined by RT-PCR and qPCR. Protein level ...
DOI:
10.1016/j.lfs.2018.10.052

2. Profiling of GABAA and GABAB receptor expression in the myometrium of the human uterus

Author:
Söderhielm, Pella Cecilia; Klein, Anders Bue; Bomholtz, Sofia Hammami; Jensen, Anders A.
Source:
Life sciences 2018 v.214 pp. 145-152
ISSN:
0024-3205
Subject:
gamma-aminobutyric acid; gene expression; genes; humans; messenger RNA; myocytes; myometrium; quantitative polymerase chain reaction; receptors; reverse transcriptase polymerase chain reaction; smooth muscle; women
Abstract:
... γ-aminobutyric acid (GABA) mediates its physiological effects through the GABAA and GABAB receptors. In this study the putative expression of GABAAR and GABABR subunits in human myometrium tissue was investigated.The expression levels of the 19 GABAAR subunits (α₁–α₆, β₁–β₃, γ₁–γ₃, δ, ε, π, θ, ρ₁–ρ₃) and the three GABABR subunits (GABAB₁ₐ, GABAB₁b, GABAB₂) were characterized by RT-qPCR analysis on ...
DOI:
10.1016/j.lfs.2018.10.033

3. Clioquinol induces G2/M cell cycle arrest through the up-regulation of TDH3 in Saccharomyces cerevisiae

Author:
Yan, Chongjia; Wang, Song; Wang, Jian; Li, Hui; Huang, Zhiwei; Sun, Jing; Peng, Min; Liu, Wenbin; Shi, Ping
Source:
Microbiological research 2018 v.214 pp. 1-7
ISSN:
0944-5013
Subject:
Candida albicans; Saccharomyces cerevisiae; amino acid metabolism; anti-infective agents; cell cycle checkpoints; databases; fermentation; gene expression regulation; gene overexpression; genes; gluconeogenesis; glycolysis; interphase; mechanism of action; messenger RNA; proteins; proteomics; reactive oxygen species; redox reactions; reverse transcriptase polymerase chain reaction; two-dimensional gel electrophoresis; yeasts
Abstract:
... Clioquinol (CQ) has been used as a classical antimicrobial agent for many years. However, its mode of action is still unclear. In our study, the growth of Candida albicans and Saccharomyces cerevisiae was inhibited by CQ. It did not kill yeast cells, but shortened G1 phase and arrested cell cycle at G2/M phase. By using two-dimensional electrophoresis based proteomic approach, six proteins were fo ...
DOI:
10.1016/j.micres.2018.05.006