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... This study investigated the acute effects of hexavalent chromium (Cr(VI)) shock load at 2.5, 6 and 25 mg/L on the performance and bacterial community structures in aerobiotic activated sludge reactors. The results showed that eight-day Cr(VI) toxicity made the removal rates of COD and NH₃–N to obviously decrease in all reactors. Furthermore, the higher the Cr(VI) concentration was, the more severe ...
... Adding alkaline into an anaerobic waste activated sludge (WAS) fermentation with thermophilic bacteria pretreatment could efficiently improve short-chain fatty acids (SCFAs) accumulation to 3550 ± 120 mg COD/L. The acidification rate in combined test was 21.2%, while that was 15.6% and 10.7% in sole thermophilic bacteria pretreatment and control tests respectively. Four distinct groups of microbes ...
... Activated sludge (AS) and return activated sludge (RAS) microbial communities from three full-scale municipal wastewater treatment plants (denoted plant A, B and C) were compared to assess the impact of sludge settling (i.e. gravity thickening in the clarifier) and profile microorganisms responsible for nutrient removal and reactor foaming. The results show that all three plants were dominated wit ...
Grazielly Maria Didier de Vasconcelos; Jéssica Mulinari; Selene Maria de Arruda Guelli Ulson de Souza; Antônio Augusto Ulson de Souza; Débora de Oliveira; Cristiano José de Andrade
... The effluent from the textile industry is a complex mixture of recalcitrant molecules that can harm the environment and human health. Biological treatments are usually applied for this wastewater, particularly activated sludge, due to its high efficiency, and low implementation and operation costs. However, the activated sludge microbiome is rarely well-known. In general, activated sludges are com ...
... Soil aquifer treatment (SAT), applied after activated sludge treatment (AST), has been widely used for wastewater reclamation. AST and SAT show potential for removing micropollutants, including pharmaceuticals and personal care products (PPCPs). However, the role of sequential combination of AST and SAT on the biodegradation of PPCPs was not clear in previous studies. In this study, the removal ch ...
... This study investigated the effects of substrate composition (acetate and propionate) on synthesis of various mix of poly(hydroxybutyrate-hydroxyvalerate) (P(HB/HV)) from activated sludge, which was acclimated using a single carbon (acetate) and mixed carbons (acetate and propionate). Results of batch P(HB/HV) production tests indicated that the yield and synthesis rate of P(HB/HV) decreased as th ...
... The efficiency of petrochemical wastewater biological treatment is dependent upon complex bacterial communities. A well understanding of the structure and function of bacterial community and their association with environmental variables is essential for the elucidation of contaminant removal mechanisms and optimization of wastewater treatment processes. In this study, the bacterial communities an ...
... While the oxidation of ammonia is an integral component of advanced aerobic livestock wastewater treatment, the rate of nitrification by ammonia-oxidizing bacteria is drastically reduced at colder temperatures. In this study we report an acclimated lagoon nitrifying sludge that is capable of high rates of nitrification at temperatures from 5°C (11.2mg N/g MLVSS/h) to 20°C (40.4mg N/g MLVSS/h). The ...
... In this study, integrated fixed-film activated sludge (IFAS) microbial development and degradation efficiency were investigated for oil sands process-affected water (OSPW) remediation. IFAS microbial community was characterized using 454 high-throughput 16S rRNA gene pyrosequencing that revealed that the IFAS seed sludge (activated sludge [AS] from the Gold Bar Wastewater Treatment Plant [GBWTP]) ...
Acidobacteria; Bacteroidetes; Firmicutes; Nitrospirae; Proteobacteria; activated sludge; bacterial communities; biodiversity; chemical oxygen demand; community structure; genes; humans; municipal wastewater; nitrates; nitrification; nitrites; oxidation; total nitrogen; wastewater treatment; water; China
Abstract:
... Municipal wastewater treatment plants (WWTPs) use functional microorganisms in activated sludge (AS) to reduce the environmental threat posed by wastewater. In this study, Illumina NovaSeq sequencing of 16S rRNA genes was performed to explore the microbial communities of AS at different stages of the two WWTP projects in Shenzhen, China. Results showed that Proteobacteria, Bacteroidetes, Acidobact ...
... We investigated the effect of chemical oxygen demand (COD)/N ratio on polyhydroxybutyrate (PHB) accumulating ability in an anaerobic/aerobic cycle sequential batch reactor (SBR). Compared the COD/N ratio of 10, 20, 50, and 125, the COD/N of 125 was the most suitable nutritional proportion. When COD was 1,200 mg/L and COD/N/P was 1,200/9.6/30, activated sludge PHB synthesis reached a maximum of 64. ...
Acidobacteria; Actinobacteria; Bacteroidetes; Chloroflexi; Firmicutes; Nitrosomonas; activated sludge; amino acids; bacterial communities; biogenesis; carbohydrates; cell walls; chemical oxygen demand; community structure; energy; environmental factors; gene dosage; genetic variation; high-throughput nucleotide sequencing; metabolism; municipal wastewater; nitrifying bacteria; pH; phylotype; ribosomal RNA; signal transduction; wastewater treatment; China
Abstract:
... Efforts to understand the environmental and biological factors that influence the dynamics of microbial communities have received substantial attention in microbial ecology. In this study, Illumina MiSeq high-throughput sequencing technology was used to examine the microbial community structure of activated sludge in municipal wastewater treatment systems (Chuzhou city, China). Overall, Proteobact ...
... Propidium monoazide (PMA) has been used to determine viable microorganisms for clinical and environmental samples since selected naked DNA which was covalently cross-linked by this dye could not be PCR-amplified. In this study, we applied PMA to the activated sludge samples composed of complex bacterial populations to investigate the viability of human fecal bacteria and to determine the heat-tole ...
... Microbial biocathodes are gaining interest due to their low cost, environmental friendliness and sustainable nature. In this study, a microbial consortium was enriched from activated sludge obtained from a common textile effluent treatment plant in the absence of organic carbon source to produce an electroactive biofilm. Chronoamperometry method of enrichment was carried out for over 70 days to se ...
... Dynamics of the composition of the microbial community was studied during start-up of a single-stage completely mixed constant flow laboratory setup for ammonium removal by the nitritation/anammox process from the filtrate of digested sludge of the Kuryanovo wastewater treatment plant (KWTP), Moscow. To decrease the period of the start-up, the setup was initially inoculated with two types of activ ...
... The anaerobic-anoxic-aerobic (A2O) process is a highly efficient sewage treatment method, which uses complex bacterial communities. However, the effect of copper on this process and the bacterial communities involved remains unknown. In this study, a systematic investigation of the effect of persistent exposure of copper in the A2O wastewater treatment system was performed. An A2O device was desig ...
... The effects of wood vinegar (WVG) on microbial activity and communities of activated sludge were investigated in a sequencing batch reactor (SBR) process. Results showed that the optimal WVG concentration was 4 μL/L when the pollutants removal efficiency and microbial activity were promoted by a WVG dilution factor of 1000. WVG could reduce the increase in microbial species richness, which led to ...
... This research investigated the effects of ciprofloxacin (CIP) (0.5, 5, and 20 mg/L) on SBR systems under different carbon source conditions. Microbial community abundance and structure were determined by quantitative PCR and high-throughput sequencing, respectively. The biodegradation production of CIP and possible degradation mechanism were also studied. Results showed that CIP had adverse effect ...
... Biotransformation of fluorotelomer alcohols (FTOHs) in wastewater treatment plants (WWTPs) can release toxic intermediates and perfluorinated carboxylic acids (PFCAs) to the aqueous environment. However, little information is known about the role of relevant microbial activity (i.e., autotrophs and/or heterotrophs) in biotransformation of FTOHs. Additionally, the dynamics of microbial community in ...
... An integrated OCO reactor was used to investigate the performance and microbial community successional changes under long-term exposure to relatively low levels of ZnO nanoparticles (NPs). Relatively higher concentrations of ZnO NPs (1.5 mg L⁻¹) could adversely affect the nitrogen and phosphorus removal in the reactor. The diversity and richness of the microbial communities chronically declined wi ...
... Vermicomposting can significantly attenuate antibiotic resistance genes (ARGs) in the excess activated sludge (EAS). However, the effect of earthworms, especially the effect of gut digestion as a critical step in the vermicomposting process, remains unclarified. The purpose of this study was to investigate the response of ARGs (cell-free and -associated) in EAS to gut digestion of earthworms and t ...
... The wastewater from coal-mine industry varies greatly and is resistant to biodegradation for containing large quantities of inorganic and organic pollutants. Microorganisms in activated sludge are responsible for the pollutants’ removal, whereas the microbial community composition and structure are far from understood. In the present study, the sludges from five coal-mine wastewater treatment plan ...
... Poly- and perfluorinated alkyl substances (PFASs) are groups of persistent toxic substances that have been commonly detected in wastewater treatment plants (WWTPs). In some cases, the activated sludge (AS) in WWTPs will encounter special wastewaters containing PFASs up to tens of milligram per liter (mg L⁻¹). However, under this condition, the potential impacts of PFASs on AS process remain unclea ...
... The selective enrichment culture technique is widely used in domestication of pollutant-degrading microorganisms. However, very little information is available regarding the evolution and interaction of microbial communities during the domestication. For the first time, successional variation of bacterial community and metabolism were investigated during the enrichment culture of a phthalate-degra ...
... Effects of hydraulic retention time (HRT) and the chemical oxygen demand/nitrogen (COD/N) ratio on the microbial community composition of integrated fixed-film activated sludge (IFAS) and its ability to treat raw and ozonated oil sands process-affected water (OSPW) were evaluated. After 11 months of HRT and ammonium optimization, 54.56% of the COD and 30.20% of the acid extractable fraction (AEF) ...
Acidobacteria; Chloroflexi; activated sludge; bacterial communities; biomarkers; community structure; environmental factors; factor analysis; ribosomal DNA; wastewater treatment; China
Abstract:
... Overall understanding of microbial community structure in activated sludge (AS) system at regional level is of great significance for operation regulation of wastewater treatment plants (WWTPs). In this study, 110 AS samples from 21 cities in different Chinese regions were analyzed based on high-throughput 16S ribosomal DNA (rDNA) sequencing to explore effects of different regions on microbial com ...
... Tetramethylammonium Hydroxide (TMAH) is widely used as a developer in the thin-film transistor liquid crystal display (TFT-LCD) industry, which is a corrosive alkaline and neuronal toxic compound. In this study, the performances of combined hydrolysis acidification-aerobic and anaerobic batch reactors for synthetic TMAH wastewater treatment were evaluated. It also investigated the real TMAH wastew ...
... Quaternary ammonium compounds have gained widespread attention due to their extensive enrichment in waste activated sludge (WAS) and potentially adverse effect to anaerobes. This study selected benzalkonium chlorides (BACs) as model to reveal the responses of anaerobic digestion of WAS to long-term stress of BACs. Results showed that the solubilization enhancement of WAS contributed by BACs was th ...
... The main objective of this work was to investigate the influence of different oxygen supply patterns on poly-β-hydroxybutyrate (PHB) yield and bacterial community diversity. The anaerobic–aerobic (A/O) sequencing batch reactors (SBR1) and feast–famine (F/F) SBR2 were used to cultivate activated sludge to produce PHB. The mixed microbial communities were collected and analyzed after 3 months cultiv ...
... This study aims to investigate the effect of adding magnetic powder in the sequencing batch reactor (SBR) on the reactor performance and microbial community. Results indicated that, the magnetic activated sludge sequencing batch reactor (MAS-SBR) had 7.76% and 4.76% higher ammonia nitrogen (NH4+-N) and chemical oxygen demand (COD) removal efficiencies than that of the conventional SBR (C-SBR). The ...