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Anemone (plant); Arctotis; Gazania; agricultural machinery and equipment; air flow; awns; chaff; planting; seeds
Abstract:
... Some types of seed have appendages that, unless removed, interfere with seed conditioning, storage, and planting operations. An experimental seed thresher that is capable of removing difficult-to-remove seed appendages, such as awns, pappus, hair, and tails is described. Test results from threshing trials using marigold, gazania, arctotis, and anemone seed are reported. The thresher uses a series ...
... Hepatica acutiloba and Trillium nivale are spring—flowering deciduous forest herbs whose seeds are dispersed by ants. We studied patterns of seed dispersal by ants and seed predation by rodents of these two herbs at three spatial scales: (1) among three woodlots that differed in density and size of Hepatica and/or Trillium populations, (2) between two sites within each woodlot located inside and o ...
Anemone (plant); Rosa multiflora; Sedum; leaves; mass spectrometry; plant taxonomy; principal component analysis; pyrolysis; sampling; strains
Abstract:
... Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R. multiflora var. platyphylla, Sedum kamtschaticum, S. takesimense, S. sarmentosum ...
... Phylogenetic relationships within Clematis, including Naravelia, Archiclematis and Clematopsis, were analysed using nucleotide sequences of chloroplast DNA [(1) matK and trnK introns; (2) atpB-rbcL spacer; (3) rpoB-trnC spacer; (4) psbA-trnH-trnQ spacer; (5) rbcL-accD spacer] and the nuclear ITS regions. The phylogenetic trees resulting from these analyses suggested nine major clades. The genera A ...
... • Polyploidization and chromosomal rearrangements are recognized as major forces in plant evolution. Their role is investigated in the disjunctly distributed northern hemisphere Hepatica (Ranunculaceae). • Chromosome numbers, karyotype morphology, banding patterns, 5S and 35S rDNA localization in all known species were investigated and interpreted in a phylogenetic context established from nuclear ...
... • Here, the diversity of arbuscular mycorrhizal (AM) fungi was determined in a boreal herb‐rich coniferous forest in relation to environmental variables. • Root samples of five plant species (Fragaria vesca, Galeobdolon luteum, Hepatica nobilis, Oxalis acetosella and Trifolium pratense) were analysed from stands differing in age and forest management intensity. • Thirty‐four Glomeromycota taxa (sm ...
... 1. Deceptive species are a characteristic trait of insect–plant mutualisms. Deception of pollinators is essential for reproduction in diverse plant species; however, mimicry in seed dispersal is almost unknown. Myrmecochory, the dispersal of diaspores by ants, is a mutualism in which the elaiosome, a lipid‐rich seed appendage, works as an ant reward. This coevolved interaction may be exploited by ...
Anemone (plant); adults; climate change; herbs; microclimate; plant communities; seeds; soil water; temperature; woodlands; Southeastern United States
Abstract:
... The ubiquitous transition of plant communities across slope aspects is a well-described, but rarely tested, ecological dynamic. Aspect position is often used as a proxy for microclimate changes in moisture, light and temperature, but these abiotic drivers are seldom decoupled and very rarely manipulated across slope aspects. To investigate the mechanisms and demographic stages driving the observed ...
... This study was conducted to determine: (1) the associations between anti-Fasciola hepatica antibody levels in milk and some productive and reproductive parameters in dairy cattle, and (2) the threshold antibody level associated with loss of productivity, at both herd and individual level. Anti-F. hepatica antibodies were analysed by MM3-SERO ELISA in milk samples from the bulk tanks of 490 dairy f ...
... A sheep trial was performed to evaluate two diagnostic assays, a faecal egg count reduction test (FECRT) and a coproantigen reduction test (CRT), for the diagnosis of resistance of Fasciola hepatica to triclabendazole (TCBZ). The FECRT defines successful TCBZ treatment as a 95% or greater reduction in fluke faecal egg counts (FECs) at 14 days post-treatment (dpt). The CRT defines effective TCBZ tr ...
... The emission of Fasciola hepatica eggs in faeces is usually subject to oscillations along time in animals as well as humans. Thus, looking for alternative biological markers reflecting eggs shed per gram of faeces (epg) with lower oscillations may be useful. This study analyzes the possible relationship between liver-fluke uterus area and epg. Uterus area (UA) development of adult F. hepatica obta ...
... We aimed to study the composition of herb layer in old Estonian boreonemoral forests (Aegopodium and Hepatica forest site-types) and estimate factors that influence the vegetation. Besides forest management and other anthropogenic disturbances, stand age, geographic location and tree species composition were analysed as potential driving factors. We surveyed 169 forests, mostly as a contrasting pa ...
Anemone (plant); Fasciola gigantica; Fasciola hepatica; NAD (coenzyme); NADH dehydrogenase; cytochrome-c oxidase; genetic relationships; hosts; mitochondrial DNA; nucleotide sequences; phylogeny; China; Niger; United States
Abstract:
... The present study examined sequence variability in a portion of the mitochondrial cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase subunits 4 and 5 (pnad4 and pnad5) among 39 isolates of Fasciola spp., from different hosts from China, Niger, France, the United States of America, and Spain; and their phylogenetic relationships were re-constructed. Intra-species sequence variations were ...
... In the current study, three independent trials directly compared Fasciola gigantica and Fasciola hepatica infection of ITT sheep. In all trials, F. hepatica infection resulted in higher worm burden recoveries and greater physiological damage to ITT sheep. Developmental differences of the two Fasciola species were also observed during the first twelve weeks of a primary infection, where the migrati ...
... The main objective of this study was to investigate whether cholelithiasis in sheep is related to parasitism or other commonly observed disorders such as liver abscesses. Additionally, the features of the observed biliary calculi are described. The livers of 254 randomly selected clinically healthy adult dairy sheep were used. All visible concretions in the bile were considered as stones. Based on ...
... In the winter of 2008, cattle on a farm in the province of Neuquen, Argentina died from subacute and chronic liver fluke disease despite four previous treatments with Triclabendazole (TCBZ). In the spring of 2009, a preliminary efficacy test revealed good performance using nitroxynil, whereas TCBZ efficacy was only 18% by egg counts of Fasciola eggs in the faeces. Resistance to Fasciola hepatica t ...
... A sheep trial was performed to standardise a coproantigen reduction test (CRT) protocol for the diagnosis of resistance to triclabendazole (TCBZ) in Fasciola hepatica). The CRT employs the BIO K201 Fasciola coproantigen ELISA (Bio-X Diagnostics, Jemelle, Belgium) to test for the presence of F. hepatica coproantigens in a faecal sample. If it is coproantigen-positive, the CRT protocol recommends th ...
Anemone (plant); Aphaenogaster; climate; flowering; foraging; fruit set; mutualism; phenology; plant response; seed dispersal; spring; temperature
Abstract:
... Species-specific climate responses within ecological communities may disrupt the synchrony of co-evolved mutualisms that are based on the shared timing of seasonal events, such as seed dispersal by ants (myrmecochory). The spring phenology of plants and ants coincides with marked changes in temperature, light and moisture. We investigate how these environmental drivers influence both seed release ...
... The population dynamics and prevalence of Fasciola hepatica in Galba truncatula were investigated on the Teagasc hill sheep farm in Country Mayo, Ireland from August 2006 until March 2008. Galba truncatula (n=974) were collected at fortnightly intervals in 4 snail habitats on the farm, their relative density and mean monthly rainfall and temperature were recorded. Snail abundance was associated wi ...
... The aim of this study was to develop an Egg Hatch Assay (EHA) test for the detection of triclabendazole (TCBZ) resistance in Fasciola hepatica. A number of fluke isolates were used, of differing sensitivity to TCBZ. Eggs were exposed to solutions of triclabendazole sulphoxide (TCBZ.SO) for 14 days, then triggered to hatch. Egg development was divided into 6 distinct and easily identifiable stages: ...
... To investigate their antiviral potential, extracts from 126 plants grown in the Qinling region of China belonging to 103 plant species of 36 families were evaluated for antiviral activity against Tobacco mosaic virus. The activity of each plant extract on infection and replication was determined by local lesion and leafâdisc methods. Extracts from nine species [Lactuca tatarica (Linn.) C. A. Mey ...
Anemone (plant); Fasciola hepatica; blood sampling; drug formulations; drugs; liquid chromatography; mass spectrometry; metabolites; scanning electron microscopy; sheep
Abstract:
... The pharmacokinetic (PK) parameters of artesunate, artemether and their metabolites dihydroartemisinin (DHA) and dihydroartemisinin-glucuronide (DHA-glucuronide) were determined in sheep naturally infected with Fasciola hepatica. Sheep were treated either with artesunate (intramuscular (i.m.): 40 and 60mg/kg) or artemether (i.m.: 40 and 160mg/kg; oral: 80mg/kg). Blood samples were withdrawn at sel ...
... In order to investigate cytolytic activity in the testis of Fasciola hepatica, flukes belonging to several different triclabendazole (TCBZ)-sensitive and TCBZ-resistant isolates, and wild-type flukes from field infections, were studied by light and electron microscopy with a view to identifying sites of heterophagy and macromolecular hydrolysis. At the periphery of the testis tubules in all the fl ...
... This paper presents the ecology, sozological category, distribution, habitats, threats, management and monitoring, conservation methods of the medicinal plant species in the west part of the Bucegi Mountains (Meridional Carpathians, Romania). In this territory were identified 275 species of medicinal plants belonging to 190 genus and 69 families. Asteraceae, Lamiaceae, Ranunculaceae, Apiaceae, Fab ...
Anemone (plant); Fasciola hepatica; Golgi apparatus; body weight; cytochrome P-450; flukes; in vivo studies; ketoconazole; metabolic inhibitors; pharmacokinetics; rats; resistance mechanisms; transmission electron microscopy; triclabendazole; ultrastructure
Abstract:
... An in vivo study in the laboratory rat model has been carried out to monitor morphological changes in adult Fasciola hepatica over a 4-day period resulting from co-treatment with triclabendazole (TCBZ) and ketoconazole (KTZ), a cytochrome P450 inhibitor. Rats were infected with the triclabendazole-resistant Oberon isolate of F. hepatica, dosed orally with triclabendazole at a dosage of 10mg/kg liv ...
Anemone (plant); Fasciola hepatica; fascioliasis; humans; hyperplasia; jaundice; lipids; membrane potential; new drugs; permeability; potassium chloride; probability
Abstract:
... Fasciola hepatica causes biliary epithelial hyperplasia and obstructive jaundice in humans and animals. Using a planar lipid bilayer technique, we further characterized the single channel property of large conductance K⁺-permeable channels that were previously identified from F. hepatica. The single channel conductance was 254.7±17.9pS under a symmetrical 200/200mM (cis/trans) KCl gradient. Open s ...
... The study aims the evaluation and scientific documentation for some floral species of spontaneous flora for understanding the ecological requirements and decorative and terapeutical characteristics; choose the most effective and rapid methods for obtaining planting material, to preserve natural biodiversity and their propagation for ornamental and medicinal purposes. The analysed species belonged ...
Anemone (plant); detection limit; flowering; ions; mass spectrometry; monitoring; quantitative analysis; rapid methods; seedlings; triterpenoid saponins; China
Abstract:
... In the present study, a microwave-assisted extraction (MAE) and high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method was developed for the simultaneous determination of seven triterpenoid saponins in Pulsatilla turczaninovii. Operational conditions of MAE were optimized using a central composite design. Multiple-reaction monitoring was employed for quantification whi ...
... Somatic chromosome numbers of 28 Korean native species in the buttercup family are observed and analyzed. The plants include 10 genera with seven endemic taxa to the Korean peninsula, and most of them are the first reports from Korean populations. In particular, the chromosome numbers for the two taxa are reported for the first time: Clematis fusca var. coreana (H. Lév) Nakai (2 n = 16) and Thalic ...
A.M. Martínez-Ibeas; M. González-Warleta; M. Martínez-Valladares; J.A. Castro-Hermida; C. González-Lanza; B. Miñambres; C. Ferreras; M. Mezo; M.Y. Manga-González
... Paramphistomosis and Fasciolosis caused by Calicophoron daubneyi and Fasciola hepatica, respectively, are frequent and important trematodoses in ruminant livestock worldwide. Both parasites use the same snail, Galba truncatula, as intermediate host. The aim of this study was to develop and validate an analytical method based on a mitochondrial DNA (mtDNA) multiplex PCR technique which would allow ...
Stauntonia hexaphylla; Symphyotrichum subulatum var. subulatum; Litsea; mountains; Kadsura; forests; Millettia; Verbena officinalis; flora; Euphorbia maculata; Physalis angulata; Celtis; Bromus catharticus var. catharticus; Rhaphiolepis; Ardisia; plants (botany); biodiversity; Anemone (plant); surveys; indigenous species; Dysphania ambrosioides; South Korea
Abstract:
... This study investigated the plant distribution in Hajo Island, Jodo-myeon, Jindo-gun, Jeollanam-do, South Korea. The surveyed plants were recorded as total 483 taxa, 100 families, 298 genera, 427 species, 1 subspecies, 52 varieties, and 3 forms. Total 1,360 pieces were obtained as evidential specimen. Through floristic survey and collection in Hajo Island, Jodo-myeon, Jindo-gun, Jeollanam-do, 1,36 ...
... The liver fluke Fasciola hepatica is one of the most important parasites in cattle farming worldwide and responsible for serious economic losses. In this study, three ELISAs for the detection of antibodies against F. hepatica were compared by analysis of serum antibody levels of 20 calves infected with different doses (50–1000metacercariae) of F. hepatica metacercariae. The commercially available ...
... Investigation of the triclabendazole (TCBZ) resistance status of populations of Fasciola hepatica in field cases of fasciolosis, where treatment failure has been reported, can be supported by histological examination of flukes collected from recently treated hosts. In TCBZ-sensitive flukes (TCBZ-S) exposed to TCBZ metabolites for 1–4days in vivo, but not in TCBZ-resistant flukes (TCBZ-R), morpholo ...
... The experiments described here were designed to characterize the status of susceptibility/resistance to albendazole (ABZ) and triclabendazole (TCBZ) of a Fasciola hepatica isolate (named CEDIVE isolate) recovered from infected sheep (Gualeguay, Argentina) and maintained under laboratory conditions. Two separate clinical efficacy experiments were performed on sheep artificially infected with the CE ...
... The liver fluke, Fasciola hepatica, is common in many parts of Great Britain. To detect liver fluke infection and to assess whether fasciolicide treatment has been successful, the faecal egg count (FEC) and faecal egg count reduction test (FECRT) are widely used. Rumen fluke is also increasingly reported from Great Britain, but its species identity is yet to be determined. Liver fluke and rumen fl ...
... Fasciolosis caused by Fasciola hepatica, is the most prevalent parasitic disease in dairy cattle from the northern region of Cajamarca, Peru. The control of this parasite is based on the use of Triclabendazole (TCBZ), a drug that has been used for more than fifteen years in this area. Recent studies, however, have reported a lack of clinical efficacy after treating dairy cattle. This research was ...
Anemone (plant); angiogenesis; apoptosis; colorectal neoplasms; humans; in vivo studies; roots; traditional medicine
Abstract:
... Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angio ...
... This study aims to investigate the flora of 15 islands in Yeonggwang, Shinan, and Mokpo of the Jeollanamdo province and the distribution of major plants in order to use the results as fundamental data for studies on plants in islands. Field surveys were performed 25 times from 2004 to 2010 to investigate the flora in these regions. A total of 793 taxa including 123 families, 421 genera, 695 specie ...
Anemone (plant); chemical structure; chromatography; glycophospholipids; spectroscopy
Abstract:
... A new cerebroside, rivulacerebroside (1), was isolated from Anemone rivularis by repeated column chromatography. Its structure was assigned by spectroscopic data interpretation. ...
Anemone (plant); Arisaema; Asarum; Goodyera; Hexastylis; Rhododendron; Trillium; acid deposition; air pollution; canopy; climate; cutting; data collection; decision making; demography; drought; environmental factors; forest damage; forest trees; forests; herbaceous plants; herbs; humans; insects; invasive species; land use; life history; logging; managers; pathogens; physiology; species diversity; wind; woodlands; Appalachian region
Abstract:
... We synthesized the current information on mesophytic cove forests in the southern Appalachians, assessed the range of variation in herb species composition and diversity in stands with different disturbance histories and environmental conditions, identified key knowledge gaps, and suggested approaches to fill these knowledge gaps. The purpose of this synthesis was to provide information to forest ...
Anemone (plant); Funaria hygrometrica; Marchantia polymorpha; Polytrichum; Rhytidiadelphus; chemical composition; lichens; medicinal properties; mosses and liverworts; traditional medicine; Central European region; Europe
Abstract:
... Ubiquitous bryophyte species are reported from European, American and Asian ethnopharmacies. Some of their traditional medicinal uses are similar in distant and isolated cultures, and moreover, medicinal properties of some bryophytes are currently confirmed as justified by their chemical constituents. Aims of the work: we identify bryophytes listed in a medicinal and botanical work from 1600, and ...
... Post‐mining restoration sites often develop novel ecosystems as soil conditions are completely new and ecosystem assemblage can be spontaneous even on afforested sites. This study presents results from long‐term monitoring and evaluation of an afforested oil‐shale quarry in Estonia. The study is based on chronosequence data of soil and vegetation and comparisons are made to similar forest site‐typ ...
... Anemone is a representative spring ephemeral in Korea, flushing immediately after the snow melts and flowering in early spring. This study investigated the spring flush, stem growth, flowering, and fruiting of three Anemone species (A. amurensis, A. raddeana and A. koraiensis) in a montane deciduous forest on Mt. Joongwang over two consecutive years (2014 and 2015). We sought to expand our underst ...
Anemone (plant); amplified fragment length polymorphism; conifers; genetic variation; habitats; mixed forests; mountains; population structure; shrubs; understory; weeds; China; Japan
Abstract:
... Anemone shikokiana (Makino) Makino, which is distributed only in Mt. Kunyu and Mt. Lao of Shandong Province in China and Mt. Shichui of Shikoku in Japan above 600 m in elevation, is a rare plant. This species has two obviously different habitats, the shrubs in mountain top and understory of the conifer and broadleaf mixed forest. In this study, the habitats and genetic diversity were analyzed for ...
... Pulsatilla has been separated from Anemone by many authors. However, molecular phylogenies show that this genus, along with Barneoudia, Knowltonia, Hepatica and Oreithales, is nested with Anemone. For this reason, Pulsatilla is better treated as a section of Anemone. For the Chinese Pulsatilla species, nine species names are already available in Anemone, and the remaining names of three species an ...
... The circumscription and phylogenetic relationships of the Anemone section Begoniifolia have long been in dispute. Here, we use molecular data and palynological and cytological observations to test the monophyly of the section and evaluate phylogenetic relationships within the section. Three pollen types were observed in the section: Anemone sumatrana is 3-colpate; A. hokouensis, A. howellii, and A ...
... A method for secondary somatic embryogenesis was developed on embryos derived from embryogenic callus formed on Hepatica nobilis seedlings. Somatic embryogenesis (SE) was induced on seedlings (on the hypocotyl and epicotyl parts) grown on the Murashige and Skoog (1962) medium (MS) supplemented with 1 µM naphthaleneacetic acid (NAA), and/or 0.1 µM 6-benzyladenine (BA) and on medium without plant gr ...
... The changes in tree layer saplings, shrub, field and moss layer in Järvselja Nature Reserve virgin forest compartment (JS226) were analysed on the ground of data collected in 1955, 1985, 1993 and 2012. Four forest stands (sub-compartments) were studied; in every stand one 4 × 4 m sample area was established: area A1 represented Hepatica nobilis and Anemone nemoralis dominanted spruce stand, area A ...
... Anemone sensu lato (including Pulsatilla and Hepatica), tribe Anemoneae (Ranunculaceae), is arranged into two subgenera, Anemone and Anemonidium, with basic chromosome numbers x = 8 and x = 7, respectively. We elucidated the level of divergence of 5S rDNA unit arrays between the subgenera, determined intra‐individual and interspecific sequence variation and tested 5S rDNA phylogenetic signal in re ...