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... Unraveling the molecular interactions governing the first contact between parasite and host tissues is of paramount importance to the development of effective control strategies against parasites. In fasciolosis, a foodborne trematodiasis caused mainly by Fasciola hepatica, these early interactions occur between the juvenile worm and the host intestinal wall a few hours after ingestion of metacerc ...
... A previous study based on mitochondrial DNA markers reported the presence of Fasciola hepatica in Algeria. However, a precise species identification is still required. In this report, a total of 68 Fasciola isolates, collected from high-plateau (Bordj-Bou-Arreridj) and steppe (Djelfa) areas of Algeria, were identified at the species level by multiplex PCR and PCR-restriction fragment length polymo ...
... All 225 Fasciola flukes obtained from domestic animals (73 cattle, 7 sheep and 1 pig) of 18 distinct geographic areas in Ecuador-South America, were identified as Fasciola hepatica, based on molecular analyses of nuclear pepck and pold genes, and mitochondrial nad1gene as well as the morphological observation of sperm within the seminal vesicles. Fasciola gigantica and parthenogenic Fasciola forms ...
... BACKGROUND: Fasciola hepatica is an important zoonotic parasite that causes fasciolosis in a broad range of animals. No information is available about the prevalence of F. hepatica in Père David’s deer (Elaphurus davidianus), an endangered species in the world. Therefore, the purpose of the study was to evaluate the prevalence of fasciolosis in Père David’s deer in the Dafeng Elk National Natural ...
Issa Sy; Lena Margardt; Emmanuel O. Ngbede; Mohammed I. Adah; Saheed T. Yusuf; Jennifer Keiser; Jacqueline Rehner; Jürg Utzinger; Sven Poppert; Sören L. Becker
... Fascioliasis is a neglected trematode infection caused by Fasciola gigantica and Fasciola hepatica. Routine diagnosis of fascioliasis relies on macroscopic identification of adult worms in liver tissue of slaughtered animals, and microscopic detection of eggs in fecal samples of animals and humans. However, the diagnostic accuracy of morphological techniques and stool microscopy is low. Molecular ...
... BACKGROUND: Mountain environments are fragile socio-ecological systems and the conservation of their biological and cultural diversities— seen as co-evolving, strongly intertwined entities—represents a crucial issue for fostering their sustainability. Very few ethnobiological studies have assessed in the mountainous regions of Europe how local botanical knowledge, which represents a vital portion ...
Anemone (plant); Cervus dama; Fasciola hepatica; Rusa unicolor; adults; area; bile ducts; controllers; deer; definitive hosts; fallow; farmers; farms; hunters; infection; liver; liver flukes; population; rearing; vertebrate pests; Australian Capital Territory; New South Wales
Abstract:
... To determine the extent to which wild deer are contributing in the transmission of Fasciola hepatica (liver fluke) livers from deer shot by hunters, farmers undertaking population control on their farms and vertebrate pest controllers were collected and frozen. The livers were later thawed, sliced and examined for the presence of adult flukes or evidence of past infection. Livers from 19 deer were ...
Jürgen Aosaar; Tiia Drenkhan; Kalev Adamson; Kristiina Aun; Hardo Becker; Mikko Buht; Rein Drenkhan; Merit Fjodorov; Katrin Jürimaa; Gunnar Morozov; Laura Pihlak; Kätlin Piiskop; Taavi Riit; Mats Varik; Rait Väär; Marek Uri; Veiko Uri
... Stumpwood as a resource of renewable bioenergy has considerable potential in the Nordic and Baltic countries. So far, however, it has largely been left unutilised in Estonia. This study reports the results of the height growth, needle and twig traits, and Heterobasidion sp. and Armillaria sp. infection in emerging Norway spruce (Picea abies) stands after stump harvesting. After six and eight growi ...
Anemone (plant); chromosome number; geographical distribution; host specificity; indigenous species; lectotypes; phylogeny; New Zealand; South America
Abstract:
... A rational taxonomic circumscription of genera in tribe Anemoneae (Ranunculaceae) is briefly discussed. It is concluded that, in view of the morphological diversity of the group and recent molecular phylogenetic findings, a moderately narrow approach to the re-circumscription of genera earlier included in Anemone sensu lato is preferable, in particular, with the recognition of the lineage with the ...
... The increasing availability of volunteered geographic information (VGI) enables novel studies in many scientific domains. However, inconsistent VGI can negatively affect these studies. This paper describes a workflow that checks the consistency of Volunteered Phenological Observations (VPOs) while considering the synchrony of observations (i.e., the temporal dispersion of a phenological event). Th ...
... This paper analyses long-term (1960–2015) onset of flowering in 16 native terrestrial plants (11 of them produce important allergens) recorded in different parts of the Czech Republic (southern, central and northern part) in relation to the North Atlantic Oscillation (NAO) index of the preceding winter and thermal data—growing degree-days (GDD) and soil temperature. Flowering occurred significantl ...
... The changes in tree layer saplings, shrub, field and moss layer in Järvselja Nature Reserve virgin forest compartment (JS226) were analysed on the ground of data collected in 1955, 1985, 1993 and 2012. Four forest stands (sub-compartments) were studied; in every stand one 4 × 4 m sample area was established: area A1 represented Hepatica nobilis and Anemone nemoralis dominanted spruce stand, area A ...
... Ranunculaceae are a nearly cosmopolitan plant family with the highest diversity in northern temperate regions and with relatively few representatives in the tropics. As a result of their position among the early diverging eudicots and their horticultural value, the family is of great phylogenetic and taxonomic interest. Despite this, many genera remain poorly sampled in phylogenetic studies and ta ...
... Merosity, indicating the basic number of floral organs such as sepals and petals, has been constrained to specific and stable numbers during the evolution of angiosperms. The ancestral flower is considered to have a spiral arrangement of perianth organs, as in phyllotaxis, the arrangement of leaves. How has the ancestral spiral evolved into flowers with specific merosities? To address this questio ...
... Stumps of conifer trees are a prospective source of bioenergy and stump harvesting is a novel practice in forestry management in the Baltic and Nordic countries. However, as stump harvesting may cause possible environmental risks there has emerged a clear need for research focusing on sustainable forest management.Three Norway spruce (Picea abies) clear-cut areas on different soils in Estonia were ...
Anemone (plant); alleles; cultivars; gene flow; genetic markers; genetic variation; heterozygosity; loci; microsatellite repeats; population structure; Japan
Abstract:
... Premise of the study: Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow. Methods and Results: Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged fro ...
... Anemone sensu lato (including Pulsatilla and Hepatica), tribe Anemoneae (Ranunculaceae), is arranged into two subgenera, Anemone and Anemonidium, with basic chromosome numbers x = 8 and x = 7, respectively. We elucidated the level of divergence of 5S rDNA unit arrays between the subgenera, determined intra‐individual and interspecific sequence variation and tested 5S rDNA phylogenetic signal in re ...
... Post‐mining restoration sites often develop novel ecosystems as soil conditions are completely new and ecosystem assemblage can be spontaneous even on afforested sites. This study presents results from long‐term monitoring and evaluation of an afforested oil‐shale quarry in Estonia. The study is based on chronosequence data of soil and vegetation and comparisons are made to similar forest site‐typ ...
Anemone (plant); amplified fragment length polymorphism; conifers; genetic variation; habitats; mixed forests; mountains; population structure; shrubs; understory; weeds; China; Japan
Abstract:
... Anemone shikokiana (Makino) Makino, which is distributed only in Mt. Kunyu and Mt. Lao of Shandong Province in China and Mt. Shichui of Shikoku in Japan above 600 m in elevation, is a rare plant. This species has two obviously different habitats, the shrubs in mountain top and understory of the conifer and broadleaf mixed forest. In this study, the habitats and genetic diversity were analyzed for ...
Anemone (plant); Funaria hygrometrica; Marchantia polymorpha; Polytrichum; Rhytidiadelphus; chemical composition; lichens; medicinal properties; mosses and liverworts; traditional medicine; Central European region; Europe
Abstract:
... Ubiquitous bryophyte species are reported from European, American and Asian ethnopharmacies. Some of their traditional medicinal uses are similar in distant and isolated cultures, and moreover, medicinal properties of some bryophytes are currently confirmed as justified by their chemical constituents. Aims of the work: we identify bryophytes listed in a medicinal and botanical work from 1600, and ...