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- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Plant physiology 1997 v.114 no.2 pp. 419-428
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
- Robert, L.S.; Levesque-Lemay, M.; Gerster, J.L.; Hong, H.P.; Keller, W.
- Plant cell reports 1999 v.18 no.5 pp. 357-362
- Arabidopsis thaliana; genetic transformation; gene transfer; transgenic plants; Agrobacterium radiobacter; developmental stages; clones; gene expression; stigma; amino acid sequences; Nicotiana tabacum; genes; Brassica napus; nucleotide sequences; enzyme activity; histochemistry; beta-glucuronidase
- ... A genomic clone, Pis G363, containing the Brassica napus stigma-expressed gene Pis 63-2 was isolated and sequenced. The coding region of Pis G363 does not possess introns and shows 82% identity to the nucleotide sequence of a gene from Arabidopsis BAC clone T01B08. A 2-kb promoter fragment from Pis G363 was fused to the coding sequence of the marker enzyme beta-glucuronidase (GUS) and introduced i ...
- Thangstad, O.P.; Gilde, B.; Chadchawan, S.; Seem, M.; Husebye, H.; Bradley, D.; Bones, A.M.
- Plant molecular biology 2004 v.54 no.4 pp. 597-611
- Arabidopsis thaliana; guard cells; recombinant DNA; transgenic plants; embryo (plant); gene expression; phloem; Nicotiana tabacum; Brassica napus; thioglucosidase; reporter genes; tobacco; enzyme activity; promoter regions; beta-glucuronidase; rapeseed
- ... A prototypical characteristic of the Brassicaceae is the presence of the myrosinase-glucosinolate system. Myrosinase, the only known S-glycosidase in plants, degrades glucosinolates, thereby initiating the formation of isothiocyanates, nitriles and other reactive products with biological activities. We have used myrosinase gene promoters from Brassica napus and Arabidopsis thaliana fused to the β- ...