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You searched for: Subject Brassica napus Remove constraint Subject: Brassica napus Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum
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1. Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

Author:
Yu Zhong; Yuwen Wang; Baojian Chen; Jinchu Liu; Dong Wang; Mengran Li; Xiaolong Qi; Chenxu Liu; Kim Boutilier; Shaojiang Chen
Source:
Journal of integrative plant biology 2022 v.64 no.6 pp. 1281-1294
ISSN:
1672-9072
Subject:
Brassica napus; Nicotiana tabacum; allotetraploidy; corn; doubled haploids; genes; haploidy; homozygosity; mutagenesis; plant biology
Abstract:
... Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is widely and efficiently used in maize and was recently extended ...
DOI:
10.1111/jipb.13244

2. Overexpression of Brassica napus cytosolic fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase genes significantly enhanced tobacco growth and biomass

Author:
Yan-yan LI; Li-na GUO; Cheng-zhen LIANG; Zhi-gang MENG; Syed TAHIRA; San-dui GUO; Rui ZHANG
Source:
Journal of integrative agriculture 2022 v.21 no.1 pp. 49-59
ISSN:
2095-3119
Subject:
Brassica napus; Nicotiana tabacum; agriculture; biomass; crop yield; flowers; leaf area; photosynthesis; plant height; starch; sucrose; tobacco
Abstract:
... Elevated activities of cytosolic fructose-1,6-bisphosphatase (cyFBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) are associated with higher yields in plants. In this study, the expression levels of the cyFBPase and SBPase genes were increased by overexpressing rape (Brassica napus) cDNA in tobacco (Nicotiana tabacum) plants. The transgenic plants co-expressing cyFBPase and SBPase (TpFS), or e ...
DOI:
10.1016/S2095-3119(20)63438-4

3. An in planta haploid induction system in Brassica napus

Author:
Yifan Li; Dan Li; Qing Xiao; Huadong Wang; Jing Wen; Jinxing Tu; Jinxiong Shen; Tingdong Fu; Bin Yi
Source:
Journal of integrative plant biology 2022 v.64 no.6 pp. 1140-1144
ISSN:
1672-9072
Subject:
Arabidopsis thaliana; Brassica napus; Nicotiana tabacum; Solanum lycopersicum; Zea mays; corn; cultivars; doubled haploids; green fluorescent protein; haploidy; membrane proteins; oilseed crops; plant biology; protein synthesis; tobacco; tomatoes
Abstract:
... Doubled haploid technology is widely used to accelerate plant breeding, but its use in the important oilseed crop Brassica napus L. is limited because B. napus haploids could only be obtained through in vitro anther or microspore cultures. Recently, maize (Zea mays) lines containing mutations in Domain of unknown function 679 membrane protein (DMP) were used as haploid inducer lines. This new hapl ...
DOI:
10.1111/jipb.13270

4. Sulfur metabolic engineering enhances cadmium stress tolerance and root to shoot iron translocation in Brassica napus L

Author:
Hala Rajab; Muhammad Sayyar Khan; Markus Wirtz; Mario Malagoli; Fariha Qahar; Rüdiger Hell
Source:
Plant physiology and biochemistry 2020 v.152 pp. 32-43
ISSN:
0981-9428
Subject:
Brassica napus; Nicotiana tabacum; biosynthesis; cadmium; cysteine; cysteine synthase; cytosol; glutathione; heavy metals; hydrogen peroxide; iron; leaves; metabolic engineering; mitochondria; oxidative stress; plant micronutrients; plant nutrition; plastids; roots; serine O-acetyltransferase; shoots; stress tolerance; sulfur; thiols; tobacco; vascular plants
Abstract:
... Serine acetyltransferase (SAT) (EC 2.3.1.30) is the rate-limiting enzyme of cysteine (Cys) biosynthesis, providing the decisive precursor for the ubiquitous defense thiol glutathione (GSH). Together with O-acetylserine (thiol) lyase (OAS-TL; EC 2.5.1.47) SAT generates Cys in the cytosol, plastids, and mitochondria of vascular plants. The current study aimed to overproduce Cys and GSH for enhanced ...
DOI:
10.1016/j.plaphy.2020.04.017

5. Cover Crop Termination Treatment Impacts Weed Suppression Potential

Author:
Erin R. Haramoto; Robert Pearce
Source:
Weed science 2019 v.67 no.1 pp. 91-102
ISSN:
0043-1745
Subject:
Brassica napus; Nicotiana tabacum; Secale cereale; Trifolium incarnatum; Triticum aestivum; Vicia villosa; aboveground biomass; biomass production; canola; conservation practices; cover crop termination; cover crops; crop rotation; crop-weed competition; growers; herbicides; mulches; plant density; rain; reduced tillage; rye; tobacco; weed control; weeds; wheat
Abstract:
... Weed management in tobacco (Nicotiana tabacum L.) is accomplished primarily with soilresidual herbicides, cultivation, and hand removal. Management practices that reduce weed emergence, like reduced tillage and cover crop mulches, may improve weed management efficacy. Depending on cover-cropping goals, growers face trade-offs in species selection and management priorities—producing weed-suppressiv ...
DOI:
10.1017/wsc.2018.75

6. Intercropping oilseed rape as a potential relay crop for enhancing the biological control of green peach aphids and aphid‐transmitted virus diseases

Author:
Rongquan Lai; Hanqing Hu; Xiaoting Wu; Jingjing Bai; Gang Gu; Jianbao Bai; Ting Zhou; Tianran Lin; Xiujin Zhong
Source:
Entomologia experimentalis et applicata 2019 v.167 no.11 pp. 969-976
ISSN:
0013-8703
Subject:
Aphidius; Brassica napus; Cucumber mosaic virus; Myzus persicae; Nicotiana tabacum; Potato virus Y; Tobacco etch virus; biological control; crop yield; disease severity; disease transmission; economic valuation; field experimentation; flue-cured tobacco; insect control; parasitoids; relay cropping; viruses; China
Abstract:
... Tobacco viruses transmitted by green peach aphids, Myzus persicae (Sulzer) (Hemiptera: Aphididae), cause severe disease in flue‐cured tobacco, Nicotiana tabacum L. (Solanaceae), in China and throughout the world. Field experiments were conducted in 2016 and 2017 in Longyan City, Fujian Province, China, to determine whether M. persicae and aphid‐transmitted virus diseases are affected by intercropp ...
DOI:
10.1111/eea.12850

7. Linking phytotechnologies to bioeconomy; varietal screening of high biomass and energy crops for phytoremediation of Cr and Cu contaminated soils

Author:
Filip Pošćić; Guido Fellet; Massimo Fagnano; Nunzio Fiorentino; Luca Marchiol
Source:
Italian journal of agronomy 2019 v.14 no.1 pp. -
ISSN:
1125-4718
Subject:
Brassica carinata; Brassica juncea; Brassica napus; Helianthus annuus; Nicotiana tabacum; Raphanus sativus; bioeconomics; biomass production; brownfields; chlorophyll; chromium; copper; cultivars; energy crops; food production; hydroponics; inbred lines; phytoremediation; polluted soils; roots; screening; shoots; toxicity
Abstract:
... Enerbiochem was a project devoted to study new strategies of industrial valorisation of high biomass crops grown on brownfields or contaminated soils not suitable for food production. Chromium and copper accumulation and toxicity were examined in different species of agronomic interest. Cultivars of Brassica carinata A. Braun (7), Brassica juncea (L.) Czern. (4), Brassica napus L. (4), Raphanus sa ...
DOI:
10.4081/ija.2019.1176

8. Mimicking the Host Regulation of Salicylic Acid: A Virulence Strategy by the Clubroot Pathogen Plasmodiophora brassicae

Author:
Mohammad Djavaheri; Lisong Ma; Daniel F. Klessig; Axel Mithöfer; Gordon Gropp; Hossein Borhan
Source:
Molecular plant-microbe interactions 2019 v.32 no.3 pp. 296-305
ISSN:
0894-0282
Subject:
Albugo candida; Arabidopsis thaliana; Brassica napus; Nicotiana tabacum; Plasmodiophora brassicae; Pseudomonas syringae pv. tabaci; Pseudomonas syringae pv. tomato; Tobacco mosaic virus; benzoic acid; canola; clubroot; enzyme activity; esterases; gene overexpression; host plants; leaves; methyl salicylate; pathogens; phenotype; plant hormones; salicylic acid; systemic acquired resistance; tobacco; transferases; transgenic plants; virulence
Abstract:
... The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens such as Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus). P. brassicae encodes a protein, predicted to be secreted, with very limited homology to benzoic acid (BA)/SA–methyltransferase, designated PbBSMT. ...
DOI:
10.1094/MPMI-07-18-0192-R

9. Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Author:
Hana Ševčíková; Zuzana Lhotáková; Jaromír Hamet; Helena Lipavská
Source:
Physiologia plantarum 2019 v.167 no.3 pp. 365-377
ISSN:
0031-9317
Subject:
Brassica napus; Fragaria vesca; Nicotiana tabacum; Solanum tuberosum; biomass production; carbon; energy; gas exchange; growth and development; in vitro culture; leaf area; leaves; nutrition; photosynthesis; plant development; plant growth; potatoes; rapeseed; roots; shoots; starch; stomatal conductance; strawberries; sugar content; sugars; tobacco; vigor; water use efficiency
Abstract:
... Rate of photosynthesis and related plant carbohydrate status are crucial factors affecting plant vigor. Sugars providing carbon and energy sources serve also as important signaling molecules governing plant growth and development through a complex regulatory network. These facts are often neglected when mixotrophic cultivation of plants in vitro is used, where artificial exogenous sugar supply hin ...
DOI:
10.1111/ppl.12893

10. Promoters pro-SmAMP1 and pro-SmAMP2 from Wild Plant Stellaria media for the Biotechnology of Dicotyledons

Author:
N. V. Madzharova; K. A. Kazakova; S. R. Strelnikova; O. A. Snycheva; E. M. Vetchinkina; L. N. Efremova; D. A. Vysotskii; A. V. Babakov; R. A. Komakhin
Source:
Russian journal of plant physiology 2018 v.65 no.5 pp. 750-761
ISSN:
1021-4437
Subject:
Arabidopsis thaliana; Beta vulgaris; Brassica napus; Linum usitatissimum; Nicotiana benthamiana; Nicotiana tabacum; Stellaria media; biotechnology; crops; genes; homozygosity; kanamycin; kanamycin kinase; promoter regions; rapeseed; sugar beet; tobacco; transgenic plants; wild plants
Abstract:
... Comparative results of the studied effectiveness of two new promoters, pro-SmAMP1 and pro- SmAMP2, from chickweed (Stellaria media L.) in various types of cultivated plants with transient expression and in stable transformants are given. The effectiveness of the promoters was evaluated through the expression of the reporter uidA gene by measuring the activity of its GUS protein product. It was fou ...
DOI:
10.1134/S1021443718040040

11. Waste or substrate for metal hyperaccumulating plants — The potential of phytomining on waste incineration bottom ash

Author:
Theresa Rosenkranz; Johannes Kisser; Walter W. Wenzel; Markus Puschenreiter
Source:
Science of the total environment 2017 v.575 pp. 910-918
ISSN:
0048-9697
Subject:
plant growth; copper; salinity; Alyssum (Brassicaceae); hazardous waste; zinc; municipal solid waste; soil; market prices; waste incineration; Nicotiana tabacum; Brassica napus; biomass production; nickel; economic feasibility; bottom ash; toxicity; Sedum plumbizincicola; hyperaccumulators
Abstract:
... Phytomining could represent an innovative low-cost technology for the selective recovery of valuable trace elements from secondary resources. In this context the potential of phytomining from waste incineration bottom ash was tested in a pot experiment. Fresh bottom ash was acidified, leached to reduce salinity and amended with organic material to obtain a suitable substrate for plant growth. Two ...
DOI:
10.1016/j.scitotenv.2016.09.144

12. Capability of the plant-associated bacterium, Ensifer adhaerens strain OV14, to genetically transform its original host Brassica napus

Author:
Dheeraj Singh Rathore; Fiona Doohan; Ewen Mullins
Source:
Plant cell, tissue, and organ culture 2016 v.127 no.1 pp. 85-94
ISSN:
0167-6857
Subject:
Agrobacterium radiobacter; Arabidopsis thaliana; Brassica napus; Ensifer adhaerens; Ensifer meliloti; Nicotiana tabacum; Oryza sativa; Solanum tuberosum; acetosyringone; bacteria; cultivars; embryophytes; explants; host range; hosts; inheritance (genetics); kanamycin; microbial communities; mutualism; parasitism; petioles; phenotype; phyllosphere; rhizosphere; symbionts; transgenes; transgenic plants
Abstract:
... Land plants exist in intimate associations with complex microbial communities across the phyllosphere, endosphere, and rhizosphere, with the latter inhabited by microbes that establish relationships with their host extending from parasitism to mutualism. For example, the rhizospheric Agrobacterium tumefaciens is pathogenic across a broad host range while its related rhizobia Sinorhizobium meliloti ...
DOI:
10.1007/s11240-016-1032-3

13. Innate and acquired components of oligophagy in the herbivorous lepidopteran, Manduca sexta

Author:
William C. Gretes; Elizabeth A. Stanwyck; Frank E. Hanson
Source:
Entomologia experimentalis et applicata 2016 v.160 no.3 pp. 259-271
ISSN:
0013-8703
Subject:
Brassica napus; Lactuca sativa; Manduca sexta; Nicotiana tabacum; Physalis alkekengi; Physalis pruinosa; Solanum tuberosum; Vigna unguiculata subsp. unguiculata; artificial diets; cowpeas; foods; glass fibers; herbivores; larvae; lettuce; potatoes; rapeseed; rearing; tobacco; vines
Abstract:
... Innate and acquired aspects of oligophagy were investigated in the tobacco hornworm, Manduca sexta L. (Lepidoptera: Sphingidae), a species normally restricted to members of the family Solanaceae. Larvae were reared in the laboratory on solanaceous species tobacco (Nicotiana tabacum L.), strawberry groundcherry (Physalis pruinosa L.), and potato (Solanum tuberosum L.), as well as the non‐solanaceou ...
DOI:
10.1111/eea.12476

14. Epigenetic Changes Accompany Developmental Programmed Cell Death in Tapetum Cells

Author:
María-Teresa Solís; Nandini Chakrabarti; Eduardo Corredor; Josefina Cortés-Eslava; María Rodríguez-Serrano; Marco Biggiogera; María C. Risueño; Pilar S. Testillano
Source:
Plant & cell physiology 2014 v.55 no.1 pp. 16-29
ISSN:
0032-0781
Subject:
epigenetics; DNA methylation; caspases; histones; DNA; Nicotiana tabacum; Brassica napus; chromatin; pollen; methyltransferases; functional status; apoptosis
Abstract:
... The tapetum, the nursing tissue inside anthers, undergoes cellular degradation by programmed cell death (PCD) during late stages of microspore–early pollen development. Despite the key function of tapetum, little is known about the molecular mechanisms regulating this cell death process in which profound nuclear and chromatin changes occur. Epigenetic features (DNA methylation and histone modifica ...
DOI:
10.1093/pcp/pct152

15. Lox-dependent gene expression in transgenic plants obtained via Agrobacterium-mediated transformation

Author:
N. Shcherbak; O. Kishchenko; L. Sakhno; I. Komarnytsky; M. Kuchuk
Source:
Cytology and genetics 2013 v.47 no.3 pp. 145-155
ISSN:
0095-4527
Subject:
Beta vulgaris; Brassica napus; Lactuca sativa; Nicotiana tabacum; Solanum tuberosum; bacteriophages; biolistics; crops; gene expression; genes; leaves; phenotype; plasmids; transfer DNA; transgenic plants
Abstract:
... Lox sites of the Cre/lox recombination system from bacteriophage P1 were analyzed for their ability to affect on transgene expression when inserted upstream from a gene coding sequence adjacent to the right border (RB) of T-DNA. Wild and mutated types of lox sites were tested for their effect upon bar gene expression in plants obtained via Agrobacterium-mediated and biolistic transformation method ...
DOI:
10.3103/S0095452713030079

16. First Report of Branched Broomrape (Orobanche ramosa) on Oilseed Rape (Brassica napus), Wild Mustard (Sinapis arvensis), and Wild Vetch (Vicia spp.) in Northern Greece

Author:
Tsialtas J. T.; Eleftherohorinos I. G.
Source:
Plant disease 2011 v.95 no.10 pp. 1322
ISSN:
0191-2917
Subject:
Brassica napus; Helianthus annuus; Nicotiana tabacum; Orobanche ramosa; Sinapis arvensis; Vicia; air temperature; corolla; cotton; crops; edge effects; host plants; leaves; lips; parasitic plants; parasitism; roots; soil depth; soil temperature; stamens; stems; straw; tobacco; trichomes; weeds; Australia; Greece
Abstract:
... Branched broomrape (Orobanche ramosa L.) is a chlorophyll-lacking, root parasitic plant that infects many crops and wild species (2). Plants are densely hairy with minute, glandular hairs, particularly on flowers and upper stems. Stems are erect, often branched just above the ground, and brown to straw yellow. Leaves are sparse, triangular, dark brown or purple, and arranged alternately mainly nea ...
DOI:
10.1094/PDIS-06-11-0462

17. Influence of electrical fields (AC and DC) on phytoremediation of metal polluted soils with rapeseed (Brassica napus) and tobacco (Nicotiana tabacum)

Author:
Ran Bi; Michael Schlaak; Eike Siefert; Richard Lord; Helen Connolly
Source:
Chemosphere 2011 v.83 no.3 pp. 318-326
ISSN:
0045-6535
Subject:
Brassica napus; Nicotiana tabacum; biomass production; crop production; electric current; electric field; electrodes; forest soils; heavy metals; pH; phytoremediation; polluted soils; rapeseed; shoots; soil texture; tobacco
Abstract:
... The combined use of electrokinetic remediation and phytoremediation to decontaminate soil polluted with heavy metals has been demonstrated in a laboratory-scale experiment. The plants species selected were rapeseed and tobacco. Three kinds of soil were used: un-contaminated soil from forest area (S1), artificially contaminated soil with 15mgkg⁻¹ Cd (S2) and multi-contaminated soil with Cd, Zn and ...
DOI:
10.1016/j.chemosphere.2010.12.052
PubMed:
21237480

18. The carboxy-terminal ER-retention motif, SEKDEL, influences the N-linked glycosylation of recombinant human α-l-iduronidase but has little effect on enzyme activity in seeds of Brassica napus and Nicotiana tabacum

Author:
Galpin, Jason D.; Clemens, Sabine; Kermode, Allison R.
Source:
Plant science 2010 v.178 no.5 pp. 440-447
ISSN:
0168-9452
Subject:
glycosidases; xylose; recombinant proteins; fucose; amino acid sequences; Nicotiana tabacum; genes; Brassica napus; rapeseed; polysaccharides; tobacco; enzyme activity; molecular farming; glycoproteins; Golgi apparatus
Abstract:
... One of the potential drawbacks of the use of plant-based systems for production of human glycoproteins is the presence of immunogenic sugars within the protein's glycans, especially xylose. This can occur as a consequence of the protein undergoing transit through the plant Golgi complex, in which modifying enzymes may convert the high-mannose N-glycans of the recombinant protein to complex forms. ...
DOI:
10.1016/j.plantsci.2010.02.004

19. Impact of Fusarium oxysporum on the holoparasitic weed Phelipanche ramosa: biocontrol efficacy under field-grown conditions

Author:
KOHLSCHMID, E.; SAUERBORN, J.; MÜLLER-STÖVER, D.
Source:
Weed research 2009 v.49 no.s1 pp. 56-65
ISSN:
0043-1737
Subject:
Brassica napus; Fusarium oxysporum; Nicotiana tabacum; Orobanche ramosa; biological control; biological control agents; biomass; environmental factors; fungi; granules; greenhouse experimentation; hemp; host plants; parasitic plants; parasitism; pellets; shoots; soil; tobacco; tomatoes; weeds; Western European region
Abstract:
... Under the changing agro-climatic conditions of western Europe, the parasitic weed Phelipanche ramosa infests host crops such as tomato, hemp, tobacco and oilseed rape at an increasing rate. A Fusarium oxysporum isolate (FOG), that had effectively reduced the parasite's incidence under controlled environmental conditions, was tested in different granular formulations (pesta granules, alginate pelle ...
DOI:
10.1111/j.1365-3180.2009.00743.x

20. Molecular characterization of ThIPK2, an inositol polyphosphate kinase gene homolog from Thellungiella halophila, and its heterologous expression to improve abiotic stress tolerance in Brassica napus

Author:
Zhu, Jin-Qi; Zhang, Jian-Tao; Tang, Ren-Jie; Lv, Qun-Dan; Wang, Qiu-Qing; Yang, Lei; Zhang, Hong-Xia
Source:
Physiologia plantarum 2009 v.136 no.4 pp. 407-425
ISSN:
0031-9317
Subject:
Brassica napus; Nicotiana tabacum; crops; drought tolerance; flowers; fruits; gene expression; genes; genetic markers; genetic transformation; leaves; mannitol; mutants; myo-inositol; oxidative stress; plant response; plasma membrane; proteins; protoplasts; reverse transcriptase polymerase chain reaction; roots; salt stress; shoots; sodium chloride; stems; stress tolerance; tobacco; transgenic plants; yeasts
Abstract:
... Inositol polyphosphate kinases play important roles in diverse cellular processes. In this study, the function of an inositol polyphosphate kinase gene homolog named ThIPK2 from a dicotyledonous halophyte Thellungiella halophila was investigated. The deduced translation product (ThIPK2) shares 85% identity with the Arabidopsis inositol polyphosphate kinase AtIPK2β. Transient expression of ThIPK2-Y ...
DOI:
10.1111/j.1399-3054.2009.01235.x
PubMed:
19470090

21. Olfactory responses of Aphidius gifuensis to odors of host plants and aphid-plant complexes

Author:
Yang, Song; Xu, Rui; Yang, Shuo-Yuan; Kuang, Rong-Ping
Source:
Insect science 2009 v.16 no.6 pp. 503-510
ISSN:
1672-9609
Subject:
Aphidius; Brassica napus; Lipaphis erysimi; Myzus persicae; Nicotiana tabacum; females; host plants; mechanical damage; odors; olfactometers
Abstract:
... The olfactory responses of Aphidius gifuensis to odors from two host plants (Nicotiana tabacum and Brassica napus ssp.) and their complexes with different infestation levels of two host aphids (Myzus persicae and Lipaphis erysimi) were respectively examined in an olfactometer. The results showed that female A. gifuensis did not respond to odors of undamaged or mechanically damaged host plants, but ...
DOI:
10.1111/j.1744-7917.2009.01282.x

22. Pea-derived vaccines demonstrate high immunogenicity and protection in rabbits against rabbit haemorrhagic disease virus

Author:
Mikschofsky, Heike; Schirrmeier, Horst; Keil, Günther M.; Lange, Bodo; Polowick, Patricia L.; Keller, Wilf; Broer, Inge
Source:
Plant biotechnology journal 2009 v.7 no.6 pp. 537-549
ISSN:
1467-7644
Subject:
Brassica napus; Nicotiana tabacum; Pisum sativum; Rabbit hemorrhagic disease virus; Solanum tuberosum; antibodies; antigens; cholera toxin; enzyme-linked immunosorbent assay; genes; immune response; immunization; messenger RNA; peas; potatoes; protein content; rabbits; selfing; structural proteins; vaccines
Abstract:
... Vaccines against rabbit haemorrhagic disease virus (RHDV) are commercially produced in experimentally infected rabbits. A genetically engineered and manufactured version of the major structural protein of RHDV (VP60) is considered to be an alternative approach for vaccine production. Plants have the potential to become an excellent recombinant production system, but the low expression level and in ...
DOI:
10.1111/j.1467-7652.2009.00422.x
PubMed:
19486322

23. Sinapis arvensis L . as a source of viruses — Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) infecting oilseed rape

Author:
Bistra A. Dikova
Source:
Acta phytopathologica et entomologica Hungarica 2008 v.43 no.1 pp. 93-99
ISSN:
0238-1249
Subject:
Aphidoidea; Brassica napus; Brassica oleracea var. botrytis; Brassica oleracea var. capitata; Cauliflower mosaic virus; Chenopodium giganteum; Chenopodium quinoa; Nicotiana rustica; Nicotiana tabacum; Sinapis arvensis; Turnip mosaic virus; Turnip yellow mosaic virus; cabbage; cauliflower; crops; enzyme-linked immunosorbent assay; farms; hybrids; indicator species; mixed infection; oilseed crops; petioles; plant diseases and disorders; plant viruses; tobacco; viruses; weeds; wild plants
Abstract:
... Plants of wild species Sinapis arvensis L . — (wild mustard) with severe mosaic symptoms were established all around different farm crops in Sofia valley and even in the Sofia suburbs in 2006. Two viruses were identified by ELISA method (DAS-ELISA) and by the indicator method. Those were the virus of the genus Caulimovirus , family Caulimoviridae — Cauliflower mosaic virus (CaMV) and a virus of th ...
DOI:
10.1556/APhyt.43.2008.1.11

24. The BnALMT1 Protein that is an Aluminum-Activated Malate Transporter is Localized in the Plasma Membrane

Author:
Ayalew Ligaba; Maki Katsuhara; Wataru Sakamoto; Hideaki Matsumoto
Source:
Plant signaling & behavior 2007 v.2 no.4 pp. 255-257
ISSN:
1559-2324
Subject:
Arabidopsis; Brassica napus; Nicotiana tabacum; Xenopus laevis; aluminum; citrates; leaves; malates; messenger RNA; oocytes; plasma membrane; polyethylene glycol; proteins; protoplasts; roots; tobacco; toxicity; transgenic plants
Abstract:
... We have previously reported that Al-induces citrate and malate efflux from P- sufficient and P-deficient plants of rape (Brassica napus L.) and that P-deficiency alone could not induce this response. Further investigation showed that the transcript of two genes designated BnALMT1 and BnALMT2 is accumulated in roots by Al-treatment. Transgenic tobacco cells (Nicotiana tabacum) and Xenopus laevis oo ...
DOI:
10.4161/psb.2.4.3868
PubMed:
19704671
PubMed Central:
PMC2634140

25. Cloning of new rubisco promoters from Brassica rapa and determination of their activity in stably transformed Brassica napus and Nicotiana tabacum plants

Author:
Anisimov, Andrey; Koivu, Kimmo; Kanerva, Anne; Kaijalainen, Seppo; Juntunen, Kari; Kuvshinov, Viktor
Source:
Molecular breeding 2007 v.19 no.3 pp. 241-253
ISSN:
1380-3743
Subject:
Brassica napus; Brassica rapa; Nicotiana tabacum; cDNA libraries; complementary DNA; cotyledons; gene expression; germination; messenger RNA; protein synthesis; quantitative polymerase chain reaction; ribulose-bisphosphate carboxylase; seedlings
Abstract:
... The aim of our study was to identify the highest expressing rubisco small subunit (RbcS) promoters (pRbcS) from the cotyledons of germinating seedlings of Brassica rapa var. oleifera to drive high-level and preferably stage-specific transgenic protein expression in Brassicaceae plants. We cloned four new pRbcS promoters using several approaches, including the construction of a cDNA library and use ...
DOI:
10.1007/s11032-006-9059-5

26. Functional genomics of microspore embryogenesis

Author:
Hosp, Julia; de Maraschin, Simone Faria; Touraev, Alisher; Boutilier, Kim
Source:
Euphytica 2007 v.158 no.3 pp. 275-285
ISSN:
0014-2336
Subject:
barley; haploidy; gametophytes; tobacco; Hordeum vulgare; androgenesis; embryo (plant); sporophytes; wheat; gene expression; microspores; genomics; Brassica napus; doubled haploids; Nicotiana tabacum; homozygosity; Triticum aestivum; rapeseed
Abstract:
... Isolated plant microspores, when stressed and cultured in vitro, can be diverted from their normal gametophytic pathway towards sporophytic development, with the formation of haploid embryos and ultimately doubled-haploid plants. This process is called androgenesis or microspore embryogenesis, and is widely used in plant breeding programmes to generate homozygous lines for breeding purposes. Proto ...
DOI:
10.1007/s10681-006-9238-9

27. Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

Author:
Srinivasan, Chinnathambi; Liu, Zongrang; Heidmann, Iris; Supena, Ence Darmo Jaya; Fukuoka, Hiro; Joosen, Ronny; Lambalk, Joep; Angenent, Gerco; Scorza, Ralph; Custers, Jan B. M.; Boutilier, Kim
Source:
Planta 2007 v.225 no.2 pp. 341-351
ISSN:
0032-0935
Subject:
Nicotiana tabacum; tobacco; transgenic plants; gene expression; transcription factors; genes; Arabidopsis thaliana; Brassica napus; cell proliferation; in vitro regeneration; somatic embryogenesis
Abstract:
... Gain-of-function studies have shown that ectopic expression of the BABY BOOM (BBM) AP2/ERF domain transcription factor is sufficient to induce spontaneous somatic embryogenesis in Arabidopsis (Arabidopsis thaliana (L.) Heynh) and Brassica napus (B. napus L.) seedlings. Here we examined the effect of ectopic BBM expression on the development and regenerative capacity of tobacco (Nicotiana tabacum L ...
Handle:
10113/23591
DOI:
10.1007/s00425-006-0358-1
PubMed:
16924539

28. Nitration of γ-tocopherol in plant tissues

Author:
Desel, Christine; Hubbermann, Eva Maria; Schwarz, Karin; Krupinska, Karin
Source:
Planta 2007 v.226 no.5 pp. 1311-1322
ISSN:
0032-0935
Subject:
Arabidopsis thaliana; Brassica napus; Nicotiana tabacum; alpha-tocopherol; animal tissues; early development; gamma-tocopherol; genes; germination; imbibition; leaves; mass spectrometry; methyltransferases; mutants; nitric oxide; plant tissues; reversed-phase high performance liquid chromatography; seeds
Abstract:
... Nitration of γ-tocopherol has been suggested to be an important mechanism for the regulation and detoxification of reactive nitrogen oxide species in animal tissues. To investigate whether this reaction does also occur in plants, reversed phase high-performance liquid chromatography (HPLC) and mass spectrometry (LC-MS) were used for analysis of 5-nitro-γ-tocopherol (5-NγT) in leaves and seeds. 5-n ...
DOI:
10.1007/s00425-007-0552-9

29. The PsEND1 promoter: a novel tool to produce genetically engineered male-sterile plants by early anther ablation

Author:
Roque, Edelín; Gómez, María D.; Ellul, Philippe; Wallbraun, Michael; Madueño, Francisco; Beltrán, José-Pío; Cañas, Luis A.
Source:
Plant cell reports 2007 v.26 no.3 pp. 313-325
ISSN:
0721-7714
Subject:
Arabidopsis thaliana; Brassica napus; Nicotiana tabacum; Solanum lycopersicum; breeding; cell viability; chimerism; cytotoxicity; gene flow; genes; genetic engineering; hybrids; microsporocytes; microsporogenesis; peas; pollen; promoter regions; seeds
Abstract:
... PsEND1 is a pea anther-specific gene that displays very early expression in the anther primordium cells. Later on, PsEND1 expression becomes restricted to the epidermis, connective, endothecium and middle layer, but it is never observed in tapetal cells or microsporocytes. We fused the PsEND1 promoter region to the cytotoxic barnase gene to induce specific ablation of the cell layers where the PsE ...
DOI:
10.1007/s00299-006-0237-z
PubMed:
17016735

30. Variability of Orobanche ramosa populations in France as revealed by cross infestations and molecular markers

Author:
Brault, M.; Betsou, F.; Jeune, B.; Tuquet, C.; Salle, G.
Source:
Environmental and experimental botany 2007 v.61 no.3 pp. 272-280
ISSN:
0098-8472
Subject:
host range; random amplified polymorphic DNA technique; Cannabis sativa; ecotypes; host plants; genetic variation; plant adaptation; hemp; Nicotiana tabacum; weeds; Brassica napus; Orobanche ramosa; genetic markers; parasitic plants; tobacco; France
Abstract:
... The dramatic spread of root holoparasite Orobanche ramosa L. (branched broomrape) has been observed in the last few years in France in fields of Brassica napus L., Nicotiana tabacum L., and Cannabis sativa L. In the face of this occurrence and considering its tremendous capacity to adapt to its host, the question about variability of O. ramosa populations arises. The virulence of broomrape populat ...
DOI:
10.1016/j.envexpbot.2007.06.009

31. Winter Cover Crops Reduce Bacterial Wilt of Flue-cured Tobacco

Author:
Ong, K.L.; Fortnum, B.A.; Kluepfel, D.A.; Riley, M.B.
Source:
Plant health progress 2007 v.8 no.1 pp. -
ISSN:
1535-1025
Subject:
Nicotiana tabacum; tobacco; Ralstonia solanacearum; bacterial wilt; disease incidence; cover crops; Secale cereale; rye; Brassica napus; canola; Vicia sativa; winter; crop rotation; crop yield; South Carolina
Abstract:
... Bacterial wilt, caused by Ralstonia solanacearum, is a serious disease for tobacco farmers in the southeastern USA. The lack of suitable land for crop rotation and increased area of production on farms has resulted in shorter rotations, and increased losses due to bacterial wilt. Cover crops are rarely grown immediately before a tobacco crop because soil fumigation for nematode control necessitate ...
Handle:
10113/4178

32. The BnALMT1 and BnALMT2 Genes from Rape Encode Aluminum-Activated Malate Transporters That Enhance the Aluminum Resistance of Plant Cells

Author:
Ligaba, Ayalew; Katsuhara, Maki; Ryan, Peter R.; Shibasaka, Mineo; Matsumoto, Hideaki
Source:
Plant physiology 2006 v.142 no.3 pp. 1294-1303
ISSN:
0032-0889
Subject:
Brassica napus; Nicotiana tabacum; Triticum aestivum; Xenopus laevis; aluminum; anions; cations; citrates; gene expression; genes; lanthanum; malates; oocytes; phosphorus; roots; shoots; tobacco; transfection; transporters; wheat; ytterbium
Abstract:
... The release of organic anions from roots can protect plants from aluminum (Al) toxicity and help them overcome phosphorus (P) deficiency. Our previous findings showed that Al treatment induced malate and citrate efflux from rape (Brassica napus) roots, and that P deficiency did not induce the efflux. Since this response is similar to the malate efflux from wheat (Triticum aestivum) that is control ...
DOI:
10.1104/pp.106.085233
PubMed:
17028155
PubMed Central:
PMC1630743

33. Enhanced Protease Inhibitor Expression in Plant Residues Slows Nitrogen Mineralization

Author:
K. Kumar; C. J. Rosen; M. P. Russelle
Source:
Agronomy journal 2006 v.98 no.3 pp. 514-521
ISSN:
0002-1962
Subject:
Brassica napus; rapeseed products; Oryza sativa; rice; nitrogen; mineralization; proteinases; proteinase inhibitors; crop residues; transgenic plants; Nicotiana tabacum; tobacco; leaves; nitrogen content; carbon nitrogen ratio; lignin; soil fertility; nutrient availability
Abstract:
... Organic N mineralization by extracellular proteases affects inorganic N availability and loss. Soil N mineralization is slowed by addition of purified protease inhibitors. We hypothesized that elevated concentrations of protease inhibitors in plant residues would reduce soil and plant residue N mineralization. Isogenic controls and transgenic plants of Brassica (Brassica napus L.), Japonicum rice ...
Handle:
10113/3629
DOI:
10.2134/agronj2005.0261

34. Presence of Unintended Agrobacterium tumefaciens Cloning Vector Sequences in Genetically Modified Plants

Author:
Katarina Björklöf; Michael Färdig; Kirsten S. Jørgensen
Source:
Biotechnology letters 2006 v.28 no.5 pp. 341-349
ISSN:
0141-5492
Subject:
Agrobacterium radiobacter; Brassica napus; Nicotiana tabacum; antibiotics; bacteria; genetic vectors; genetically modified plants; greenhouses; tobacco
Abstract:
... Agrobacterium transformation was used in the production of genetically modified plants from oilseed rape (Brassica napus) and tobacco (Nicotiana tabacum). After inoculation stop with the antibiotic timentin, a subsequent one-week treatment eliminated the vector bacterium from the oilseed rape plate explant cultures. From the tobacco, however, we recorded vector-derived signals one week after potti ...
DOI:
10.1007/s10529-005-5933-1

35. The production and release of living root cap border cells is a function of root apical meristem type in dicotyledonous angiosperm plants

Author:
Hamamoto, L.; Hawes, M.C.; Rost, T.L.
Source:
Annals of botany 2006 v.97 no.5 pp. 917-923
ISSN:
0305-7364
Subject:
Vigna unguiculata; Helianthus annuus; developmental stages; Phaseolus vulgaris; Brassica napus; Pisum sativum; Solanum melongena; Capsicum annuum; Gossypium hirsutum; Daucus carota; Nicotiana tabacum; Sesbania exaltata; Arabidopsis thaliana; apical meristems; plant development; Glycine max; Cucumis sativus; Cucumis melo; root growth; ultrastructure; Solanum lycopersicum var. lycopersicum; Petunia hybrida; Citrullus lanatus; root cap; Luffa aegyptiaca
Abstract:
... BACKGROUND AND AIMS: The root apical meristems (RAM) of flowering plant roots are organized into recognizable pattern types. At present, there are no known ecological or physiological benefits to having one RAM organization type over another. Although there are phylogenetic distribution patterns in plant groups, the possible evolutionary advantages of different RAM organization patterns are not un ...
DOI:
10.1093/aob/mcj602
PubMed:
16488922
PubMed Central:
PMC2803423

36. Ectopic expression of EFFECTOR OF TRANSCRIPTION perturbs gibberellin-mediated plant developmental processes

Author:
Ellerstrom, M.; Reidt, W.; Ivanov, R.; Tiedemann, J.; Melzer, M.; Tewes, A.; Moritz, T.; Mock, H.P.; Sitbon, F.; Rask, L.
Source:
Plant molecular biology 2005 v.59 no.4 pp. 663-681
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; transcription factors; gene transfer; seed germination; dwarfing; anthocyanins; flowering; transgenic plants; lignification; gene expression; genes; Nicotiana tabacum; Brassica napus; gibberellins; cell differentiation; xylem; gene expression regulation; tobacco
PubMed:
16244914

37. Virulence diversity among branched broomrape (O. ramosa L.) populations in France

Author:
Benharrat, H.; Boulet, C.; Theodet, C.; Thalouarn, P.
Source:
Agronomy for sustainable development 2005 v.25 no.1 pp. 123-128
ISSN:
1774-0746
Subject:
Orobanche ramosa; parasitic plants; varieties; genetic variation; virulence; Cannabis sativa; hemp; Brassica napus; Fagopyrum esculentum; buckwheat; Nicotiana tabacum; tobacco; Buddleja; host plants; polymerase chain reaction; microsatellite repeats; France

38. Cell specific, cross-species expression of myrosinases in Brassica napus, Arabidopsis thaliana and Nicotiana tabacum

Author:
Thangstad, O.P.; Gilde, B.; Chadchawan, S.; Seem, M.; Husebye, H.; Bradley, D.; Bones, A.M.
Source:
Plant molecular biology 2004 v.54 no.4 pp. 597-611
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; guard cells; recombinant DNA; transgenic plants; embryo (plant); gene expression; phloem; Nicotiana tabacum; Brassica napus; thioglucosidase; reporter genes; tobacco; enzyme activity; promoter regions; beta-glucuronidase; rapeseed
Abstract:
... A prototypical characteristic of the Brassicaceae is the presence of the myrosinase-glucosinolate system. Myrosinase, the only known S-glycosidase in plants, degrades glucosinolates, thereby initiating the formation of isothiocyanates, nitriles and other reactive products with biological activities. We have used myrosinase gene promoters from Brassica napus and Arabidopsis thaliana fused to the β- ...
PubMed:
15316292

39. Oleosin-like proteins are not present on the surface of tobacco pollen

Author:
Bots, M.; Mariani, C.
Source:
Sexual plant reproduction 2004 v.16 no.5 pp. 223-226
ISSN:
0934-0882
Subject:
Arabidopsis thaliana; pollen; fertilization (reproduction); stigma; Nicotiana tabacum; wettability; Brassica napus; oleosin; protein content; pollination
DOI:
10.1007/s00497-003-0191-7

40. The Brassica napus extA extensin gene negative regulatory region controls expression in response to mechanical stresses

Author:
Shirsat, A.H.; Thomson, H.E.C.; Elliott, K.A.
Source:
Plant, cell and environment 2003 v.26 no.10 pp. 1647-1655
ISSN:
0140-7791
Subject:
seedlings; signal transduction; Brassica napus; extensin; roots; plant damage; promoter regions; gene activation; Nicotiana tabacum; wild relatives; leaves; transcription factors; messenger RNA; phloem; histochemistry; genetic transformation; transgenic plants; DNA-binding proteins; mechanical stress; reporter genes
Abstract:
... In the present study the hypothesis that the -433 to -664 bp negative regulatory region (NRR) of the Brassica napus extA extensin promoter controls extA activation in response to externally applied weight loads was tested. When weight loads were applied to the nodal regions of transgenic tobacco plants containing extA promoter deletions fused to GUS, repression controlled by the NRR was overcome a ...
DOI:
10.1046/j.1365-3040.2003.01083.x

41. Homology-dependent DNA transfer from plants to a soil bacterium under laboratory conditions: implications in evolution and horizontal gene transfer

Author:
Tepfer, D.; Garcia-Gonzales, R.; Mansouri, H.; Seruga, M.; Message, B.; Leach, F.; Perica, M.C.
Source:
Transgenic research 2003 v.12 no.4 pp. 425-437
ISSN:
0962-8819
Subject:
biosafety; Brassica napus; transposons; rapeseed; kanamycin kinase; Daucus carota; risk assessment; Nicotiana tabacum; carrots; introns; leaves; introgression; Arabidopsis thaliana; genetic recombination; soil bacteria; Calystegia sepium; intergenic DNA; Acinetobacter; transgenes; kanamycin; antibiotic resistance; transgenic plants; reporter genes; Medicago truncatula
PubMed:
12885164

42. Spontaneous mutation frequency in plants obscures the effect of chimeraplasty

Author:
Ruiter, R.; Brande, I. van den; Stals, E.; Delaure, S.; Cornelissen, M.; D'Halluin, K.
Source:
Plant molecular biology 2003 v.53 no.5 pp. 675-689
ISSN:
0167-4412
Subject:
protoplasts; genetic transformation; transgenes; biolistics; transgenic plants; Agrobacterium radiobacter; mutation; chimerism; chlorsulfuron; acetolactate synthase; Nicotiana tabacum; Brassica napus; glufosinate; herbicide resistance; tobacco; rapeseed
Abstract:
... In this study we tested the performance of chimeraplasts, chimeric RNA/DNA oligonucleotides, for the creation of directed changes in chromosomal sequences in tobacco and oilseed rape. As target genes for chimeraplasty, the endogenous als gene and two transgenes, bar and a fusion between egfp and bar, were used. In experiments in which similar numbers of cells were treated with and without chimerap ...
PubMed:
15010606

43. Enhanced quantitative resistance to Leptosphaeria maculans conferred by expression of a novel antimicrobial peptide in canola (Brassica napus L.)

Author:
Kazan, K.; Rusu, A.; Marcus, J.P.; Goulter, K.C.; Manners, J.M.
Source:
Molecular breeding 2002 v.10 no.1/2 pp. 63-70
ISSN:
1380-3743
Subject:
genetic transformation; gene transfer; messenger RNA; Plenodomus lingam; transgenic plants; fungal diseases of plants; peptides; gene expression; Nicotiana tabacum; Macadamia integrifolia; Brassica napus; disease resistance; leaves
Abstract:
... The novel antimicrobial peptide MiAMP1, originally isolated from the seeds of Macadamia integrifolia, was constitutively expressed in transgenic tobacco and canola plants to test its effect on disease resistance. Analysis of plants transformed with 35S-MiAMP1 construct by northern and western blot analyses demonstrated the presence of MiAMP1 mRNA and the mature peptide in the transgenic plants. Th ...

44. Ethanol vapor is an efficient inducer of the alc gene expression system in model and crop plant species

Author:
Sweetman, J.P.; Chu, C.; Qu, N.; Greenland, A.J.; Sonnewald, U.; Jepson, I.
Source:
Plant physiology 2002 v.129 no.3 pp. 943-948
ISSN:
0032-0889
Subject:
alcohol dehydrogenase; chemical constituents of plants; transgenic plants; recombinant proteins; starch crops; Solanum tuberosum; gene expression; potatoes; Nicotiana tabacum; leaves; Brassica napus; reporter genes; vapors; plant morphology; ethanol; beta-glucuronidase; tobacco; plant proteins
Abstract:
... We have demonstrated that low concentrations of ethanol vapor efficiently induce the alc gene expression system in tobacco (Nicotiana tabacum cv Samsun NN), potato (Solanum tuberosum cv Solara), and oilseed rape (Brassica napus cv Westar). For many situations, this may be the preferred method of induction because it avoids direct application of comparatively high concentrations of an ethanol solut ...
PubMed:
12114549
PubMed Central:
PMC1540238

45. Maintaining methylation activities during salt stress. The involvement of adenosine kinase

Author:
Weretilnyk, E.A.; Alexander, K.J.; Drebenstedt, M.; Snider, J.D.; Summers, P.S.; Moffatt, B.A.
Source:
Plant physiology 2001 v.125 no.2 pp. 856-865
ISSN:
0032-0889
Subject:
Beta vulgaris; cytochemistry; messenger RNA; choline; methylation; salinity; Spinacia oleracea; cytosol; biochemical pathways; enzyme inhibitors; Nicotiana tabacum; oxidation; Brassica napus; phosphotransferases (kinases); enzyme activity; betaine; leaves
Abstract:
... Synthesis of the compatible osmolyte Gly betaine is increased in salt-stressed spinach (Spinacia oleracea). Gly betaine arises by oxidation of choline from phosphocholine. Phosphocholine is synthesized in the cytosol by three successive S-adenosyl-Met-dependent N-methylations of phosphoethanolamine. With each transmethylation, a molecule of S-adenosylhomo-Cys (SAH) is produced, a potent inhibitor ...
PubMed:
11161043
PubMed Central:
PMC64887

46. Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.) seeds

Author:
Alessio Scarafoni; Raffaella Carzaniga; Nick Harris; Ronald D Croy
Source:
Plant molecular biology 2001 v.46 no.6 pp. 727-739
ISSN:
0167-4412
Subject:
Brassica napus; Nicotiana tabacum; amino acids; chimerism; enkephalin; gene expression; genes; hydrophilicity; hydrophobicity; polypeptides; prediction; protein bodies; protein synthesis; seeds; tobacco; topology; transgenic plants
Abstract:
... Napin is a 2S storage protein found in the seeds of oilseed rape (Brassica napus L.) and related species. Using protein structural prediction programs we have identified a region in the napin protein sequence which forms a `hydrophilic loop' composed of amino acid residues located at the protein surface. Targeting this region, we have constructed two napin chimeric genes containing the coding sequ ...
DOI:
10.1023/A:1011675918805

47. Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis

Author:
Dehesh, K.; Tai, H.; Edwards, P.; Byrne, J.; Jaworski, J.G.
Source:
Plant physiology 2001 v.125 no.2 pp. 1103-1114
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; interspecific variation; seeds; complementary DNA; enzymes; transgenic plants; rapeseed oil; Spinacia oleracea; flowers; amino acid sequences; gene expression; Nicotiana tabacum; Brassica napus; chemical constituents of plants; lipid metabolism; Cuphea; roots; promoter regions; leaves
Abstract:
... A cDNA coding for 3-ketoacyl-acyl-carrier protein (ACP) synthase III (KAS III) from spinach (Spinacia oleracea; So KAS III) was used to isolate two closely related KAS III clones (Ch KAS III-1 and Ch KAS III-2) from Cuphea hookeriana. Both Ch KAS IIIs are expressed constitutively in all tissues examined. An increase in the levels of 16:0 was observed in tobacco (Nicotiana tabacum, WT-SR) leaves ov ...
PubMed:
11161065
PubMed Central:
PMC64909

48. Compartmentation of ATP:citrate lyase in plants

Author:
Rangasamy, D.; Ratledge, C.
Source:
Plant physiology 2000 v.122 no.4 pp. 1225-1230
ISSN:
0032-0889
Subject:
cross reaction; interspecific variation; molecular weight; biomarkers; Spinacia oleracea; cytosol; plastids; Nicotiana tabacum; ATP citrate synthase; Brassica napus; Pisum sativum; enzyme activity; immunocytochemistry; leaves
Abstract:
... Extracts prepared from young leaves of Pea (Pisum sativum), tobacco (Nicotiana tabacum), rape (Brassica napus), and spinach (Spinacia oleracea) all contained ATP:citrate lyase (ACL) activity, which was most active in rape leaflets (130 nmol min-1 g fresh weight). In rape and spinach, ACL activity was predominantly localized in the plastids (between about 78% and 90% of the total activity), whereas ...
PubMed:
10759519
PubMed Central:
PMC58958

49. Expression of Bra r 1 gene in transgenic tobacco and Bra r 1 promoter activity in pollen of various plant species

Author:
Okada, T.; Sasaki, Y.; Ohta, R.; Onozuka, N.; Toriyama, K.
Source:
Plant & cell physiology 2000 v.41 no.6 pp. 757-766
ISSN:
0032-0781
Subject:
Arabidopsis thaliana; cytochemistry; interspecific variation; transgenic plants; amino acid sequences; gene expression; pollen tubes; Nicotiana tabacum; gametocytes; Brassica napus; reporter genes; cytoplasm; pollen; promoter regions
DOI:
10.1093/pcp/41.6.757
PubMed:
10945346

50. Genetic engineering of glycinebetaine production toward enhancing stress tolerance in plants: metabolic limitations

Author:
Huang, J.; Hirji, R.; Adam, L.; Rozwadowski, K.L.; Hammerlindl, J.K.; Keller, W.A.; Selvaraj, G.
Source:
Plant physiology 2000 v.122 no.3 pp. 747-756
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; interspecific variation; osmosis; frost; biosynthesis; choline; transgenic plants; Nicotiana tabacum; genetic engineering; Brassica napus; alcohol oxidoreductases; betaine; shoots
Abstract:
... Glycinebetaine (betaine) affords osmoprotection in bacteria, plants and animals, and protects cell components against harsh conditions in vitro. This and a compelling body of other evidence have encouraged the engineering of betaine production in plants lacking it. We have installed the metabolic step for oxidation of choline, a ubiquitous substance, to betaine in three diverse species, Arabidopsi ...
PubMed:
10712538
PubMed Central:
PMC58910