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... Experiments were performed to explore the impact of sulfur nanoparticles (SNPs) on growth, Cu accumulation, and physiological and biochemical responses of oilseed rape (Brassica napus L.) inoculated with 5 mg/L Cu-amended MS medium supplemented with or without 300 mg/L SNPs exposure. Cu exerted severe phytotoxicity and inhibited plant growth. SNPs application enhanced the shoot height, root length ...
... Genotype, growth stages, and moisture regimes affect polyphenols as beneficial compounds in rapeseed with edible and medicinal properties. The aims of this study were to assess the effects of tissue, genotype background and moisture on growth, pigment composition, phenolic acids, flavonoids, antioxidant, and antiglycation activities in rapeseed. Treatments included two moisture regimes (10% field ...
... Brassica napus L. (oilseed rape) is known as one of the most important volunteer crops worldwide. Brassica napus volunteers can emerge for more than 10 years in subsequent crops. Three field experiments were carried out to compare the competitive ability and reproduction of conventional and Clearfield volunteer B. napus in spring barley under different herbicide treatments in field conditions. Her ...
Brassica napus; agronomy; case studies; crop rotation; environmental impact; faba beans; field experimentation; groundwater contamination; nitrogen; nitrogen fertilizers; profits and margins; soil minerals; Germany
Abstract:
... Agriculture is a major contributor to nitrate groundwater contamination. Hence, farmers are demanded to reduce the environmental impact but simultaneously must provide sufficient food products. One important building block for this “sustainable intensification” are appropriate cropping strategies. The potential of modified crop rotations was evaluated in a high-yielding environment in Northern Ger ...
... Exploration of the mechanisms of cadmium (Cd) activation mediated by the rhizosphere process is important to advance our understanding of Cd accumulation in plants. In this study, two oilseed rape cultivars (L338, L351) with varied Cd accumulation traits were applied and the responses of their rhizosphere ecology to Cd stress were investigated by metabolome and microbiome. The results showed that ...
Brassica napus; barley; bioturbation; community structure; conventional tillage; crop rotation; earthworms; intensive farming; long term experiments; soil; soil ecology; wheat
Abstract:
... Earthworms, which contribute to important soil functions, suffer from intensive agriculture. Their response depends among other things on the earthworm ecological group (anecic, endogeic, epigeic) and the combination of the applied farming practices. To advice on methodological adaptations that enhance earthworm-mediated soil functions, effects of different practices on earthworms need to be studi ...
... Salt stress represents one of the most problematic abiotic stress affecting plants worldwide. In saline soils, plants try to counteract the consequences of salt stress by physiological changes, leading to the decreasing of both osmotic and oxidative stresses. Plant growth-promoting bacteria (PGPB) can ameliorate plant stress response by several mechanisms. In this work, we focused on the effects o ...
... The light-harvesting chlorophyll a/b-binding proteins (Lhcb) constitute the antenna system of the photosynthetic apparatus that has an essential function in photosynthesis and modulating stress responsiveness. In this study, we identified 35 Lhcb genes (BnLhcbs) in the rapeseed (Brassica napus L.) genome, which were clustered into 8 groups. The BnLhcb genes were distributed on 15 chromosomes of ra ...
Brassica napus; Brassica rapa; Chinese cabbage; cultivars; early development; plant establishment; pollution; research; seed coat; seed germination; tetracycline; water uptake
Abstract:
... Processes of seed water absorption and germination are critical for seedling establishment. To investigate the effect of tetracycline (TC), a major-use antibiotic, at low levels (1–5 nM) on the two processes, we selected two sensitive species of Brassica seeds, including Chinese cabbage (Brassica rapa L.) and edible rape (Brassica napus L.), to establish the models of seed water absorption and ger ...
... Canola is an important temperate oil crop that can be severely affected by drought. Understanding the physiological and molecular mechanisms involved in canola tolerance to water deficit is essential to obtain drought-tolerant productive cultivars. To investigate the role of antioxidant response and the possible involvement of calcium-dependent protein kinases (CDPKs) in canola tolerance to drough ...
... Phyllobacterium brassicacearum STM196, a plant growth-promoting rhizobacterium isolated from roots of oilseed rape, stimulates Arabidopsis growth. We have previously shown that the NRT2.5 and NRT2.6 genes are required for this growth promotion response. Since these genes are members of the NRT2 family of nitrate transporters, the nitrogen assimilatory pathway could be involved in growth promotion ...
... Biodiversity is rapidly declining worldwide, with agricultural intensification being among the main drivers of this process. Effective conservation measures in agricultural landscapes are therefore urgently needed. Here we introduce a novel low-cost conservation measure called artificial field defects, i.e., areas where crop is not sown and spontaneous vegetation grows. To evaluate their biodivers ...
Brassica napus; Hymenoptera; Onobrychis; arable soils; ecosystems; flowers; morphospecies; oilseeds; parasitoids; pest control; species richness
Abstract:
... Non-crop habitats are important to support biodiversity and ecosystem services in agricultural landscapes. Whereas perennial and managed non-crop habitats have known benefits, temporary non-crop patches within arable fields (field defects) have been poorly studied. We created artificial field defects (unsown patches) in oilseed rape (Brassica napus L.) fields and evaluated their effects on parasit ...
... Silicon (Si) is known to alleviate many nutritional stresses. However, in Brassica napus, which is a highly S-demanding species, the Si effect on S deficiency remains undocumented. The aim of this study was to assess whether Si alleviates the negative effects of S deficiency on Brassica napus and modulates root sulfate uptake capacity and S accumulation. For this, Brassica napus plants were cultiv ...
... Oligopeptides (Thr–His–Leu–Pro–Lys (THLPK), His–Pro–Leu–Lys (HPLK), Leu–Pro–Lys (LPK), His–Leu–Lys (HLK), and Leu–His–Lys (LHK)) are newly identified from rapeseed napin (Brassica napus) protein-derived hydrolysates with the capability of upregulating glucose transporter-4 (GLUT4) expression and translocation. However, whether each of them enhances GLUT4 expression and translocation and their spec ...
Christopher Weidner; Sophia Edelmann; Dominik Moor; Kathrin Lieske; Christian Savini; Sara Jacchia; Maria Grazia Sacco; Marco Mazzara; Jörn Lämke; Kolja Neil Eckermann; Hendrik Emons; Joachim Mankertz; Lutz Grohmann
Brassica napus; DNA; genes; genetically modified plants; herbicide resistance; point mutation; quantitative polymerase chain reaction; radishes; somaclonal variation; North America
Abstract:
... A real-time PCR method was recently published with a claim to be specific for the detection and identification of some genome-edited oilseed rape (OSR) lines commercialised in North America. The method was designed to detect a single base mutation in the AHAS1C gene, which confers herbicide tolerance. The authors claim that the method is event-specific for the genome-edited OSR line 5715 and fulfi ...
... Mechanized transplanting of rapeseed (Brassica napus L.) blanket seedling is an effective strategy to cope with the seasonal conflict and large labor cost in rapeseed production. The sowing density is a key factor to cultivate high-quality seedlings suitable for mechanized transplanting. An experiment was conducted to investigate the effects of different sowing density levels of 638, 696, 754, 812 ...
... Biochar can increase the microbial activity of a soil and the seed yield of oilseed rape. We performed a field experiment to determine the effect of different doses of biochar and the impact of nutrient additions on microbial activity in soils with low pH (4.69). Different doses of biochar were applied to plots in 2016 (0 t/ha (control), 15 t/ha (B15), 30 t/ha (B30), or 45 t/ha (B45)), and fertili ...
... Soluble bio-based substances (SBS) may be isolated from the anaerobic digestate of the organic humid fraction of urban waste; from the whole vegetable compost made from gardening residues and from the compost obtained after aerobic digestion of a mixture of urban waste digestate, gardening residues and sewage sludge. These SBS can be used as sustainable and efficient plant biostimulants in alterna ...
... Bio‐tillage has recently been proposed as a measure to alleviate soil compaction through biopores created by cover crop roots. The objective of this study was to determine the effect of different cover crops on soil physical properties and the succeeding maize (Zea mays L.) growth in compacted soil. Four treatments, including no cover crop as a control (Con), alfalfa (Medicago sativa L.), oilseed ...
... Drought stress reduces water availability in plant cells and influences rapeseed yield. Currently, key genetic regulators that contribute to rapeseed response to drought remain largely unexplored, which limits breeding of drought-resistant rapeseed. In this study, we found that Brassica napus JASMONATE ZIM-DOMAIN 5 (BnA.JAZ5), one of the transcriptional repressors functioning in the jasmonate (JA) ...
Brassica napus; bioinformatics; cytoplasm; fluorescence; genes; genetically modified organisms; rapeseed; seed coat; sequence analysis; two hybrid system techniques; vernalization
Abstract:
... 14-3-3s are general regulatory factors (GF14s or GRFs) involved in a variety of physiological regulations in plants, including the control of flowering time. However, there are poorly relevant reports in rapeseed so far. In this study, we identified a homologous 14-3-3 gene BnGF14-2c (AtGRF2_Like in Brassica napus) in rapeseed based on bioinformatic analysis by using the sequences of the flowering ...
... Chloroplasts are essential for plant growth and development, as they play a key role in photosynthesis. The chloroplast biogenesis process is complex and its regulatory mechanism remains elusive. We characterized a spontaneous Brassica napus (rapeseed) mutant, ytg, that showed a delayed greening phenotype in all green organs and retarded growth. We identified BnaA02.YTG1 encoding a chloroplast-loc ...
... Canola (Brassica napus) is an important oil crop worldwide. The seed‐setting rate (SS) is a critical factor in determining its yield, and the development of pistils affects pollination and seed sets. However, research on seed‐setting defects has been limited owing to difficulties in the identification of phenotypes, mutations, and complex genetic mechanisms. In this study, we found a stigma defect ...
... Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed, and anthocyanin and proanthocyanidins (PAs) are major seed-coat pigments. Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed. In this study, we identified a transcription factor gene BnbHLH92a (BnaA06T ...
... Rapeseed (Brassica napus) is highly sensitive to nitrogen (N) and boron (B) deficiencies. However, little is known about the effect of N and B interaction on rapeseed growth and the underlying mechanism of B regulating N starvation-induced leaf senescence in Brassica napus. In this study, B deficiency accelerated the senescence of old leaves induced by N starvation stress in B. napus, while young ...
... Loading of the Brassica napus extract (BNE) on PLGA nanoparticle (BNE-PNP) and study its necroptotic activity in human MCF7-breast cancer cells. Double emulsion solvent evaporation methods were used for synthesis of BNE-PNP and DLS, SEM, and surface Zeta-potential analysis were applied for defining the physicochemical properties of BNE-PNP. The cytotoxic impact of BNE-PNP nanoparticles was analyze ...
... The PSII repair cycle is an important part of photosynthesis and is essential for high photosynthetic efficiency. The study of essential genes in Brassica napus provides significant potential for the improvement of gene editing technology and molecular breeding design. Previously, we identified a B. napus lethal mutant (7−521Y), which was controlled by two recessive genes (cyd1 and cyd2). BnaC06.F ...
... The NF-YA transcription factor in plants is a subunit of the NF-Y (NUCLEAR FACTOR-Y) complex, which plays important roles in plant stress responses and development. The transcripts of genes encoding NF-YA are predicted to be cleaved by members of the miR169 family. We previously identified several miR169s from rapeseed (Brassica napus) based on high-throughput sequencing. In this study, by using 5 ...
Brassica napus; Camelina sativa; DNA barcoding; Sinapis alba; bacterial communities; biodiversity; community structure; drought; microbiology; microbiome; phylogeny; rhizosphere; soil water
Abstract:
... Soil history operates through time to influence the structure and biodiversity of soil bacterial communities. Examining how different soil histories endure will help clarify the rules of bacterial community assembly. In this study, we established three different soil histories in field trials; the following year these plots were planted with five different Brassicaceae species. We hypothesized tha ...
... Drought is an environmental stress that causes severe crop loss. Drought stress can induce abscisic acid (ABA) accumulation and cytoplasmic calcium oscillation. Calcium-dependent protein kinases (CPKs) constitute a group of Ser/Thr protein kinases decoding calcium signals. However, the function and molecular mechanisms of most CPKs in oilseed rape (Brassica napus) remain unknown. Here, we report t ...
Petar Čanak; Federica Zanetti; Dušica Jovičić; Bojana Vujošević; Zlatica Miladinov; Dušan Stanisavljević; Milan Mirosavljević; Barbara Alberghini; Erika Facciolla; Ana Marjanović Jeromela
Brassica napus; Camelina sativa; cold; cultivars; drought tolerance; heat; osmotic pressure; osmotic stress; polyethylene glycol; seed germination; soil water; spring; stand establishment; vegetables; water stress; Serbia
Abstract:
... Camelina [Camelina sativa (L.) Crantz] has increased cold, heat, and drought tolerance and decreased susceptibility to diseases and pests than oilseed rape (Brassica napus L.). Because water deficit at sowing leads to unsatisfactory stand establishment due to irregular seed germination and emergence, the aim of this study was to understand the response of camelina germination under osmotic stress ...
... BACKGROUND: Brassica napus is an important agricultural species, improving stress resistance was one of the main breeding goals at present. Non-specific lipid transfer proteins (nsLTPs) are small, basic proteins which are involved in some biotic or abiotic stress responses. B. napus is susceptible to a variety of fungal diseases, so identify the BnLTPs and their expression in disease responses is ...
... Rapeseed is a significant oil-bearing cash crop. As a hybrid crop, Brassica napus L. produces a high yield, but it also has drawbacks such as a tall stalk, easy lodging, and is not suitable for mechanized production. To address these concerns, we created the DW871 rapeseed dwarf variety, which has a high yield, high oil content, and is suitable for mechanized production. To fully comprehend the dw ...
... Oilseed rape (Brassica napus L.) is cultivated worldwide, producing 11.5% of global oilseeds at an economic value of 38 billion USD in 2020. It is sensitive to phytotoxic damage from exposure to tropospheric ozone (O₃), a major air pollutant, which disrupts plant physiological processes and thus decreases biomass accumulation. As background ozone concentrations continue to increase globally, we in ...
... This study aimed to assess the impact of individual as well as combined application of Lysinibacillus macroides and Bacillus safensis in phytoremediation potential of Brassica napus grown in soil contaminated by industrial effluents. In response to five metals; copper, chromium, nickel, lead, and cadmium, results revealed that germination percentage, fresh and dry weights, and photosynthetic pigme ...
... Accumulation of microplastics (MPs) in agricultural environments has caused growing concern in recent years because of its detrimental impacts on soil quality, crop productivity and ecosystem function. This study was conducted to assess the impact of biochar on soil chemical and microbial properties in a MP‐contaminated soil under two moisture regimes. Soil was contaminated with 1% (w/w) of low‐de ...
Qing Qing Xu; Abdul Sami; Hong Zhang; Xue Zi Jin; Wen Yin Zheng; Zhe Yi Zhu; Ling Ling Wu; Yu Hang Lei; Zhi Peng Chen; Yong Li; Yan Yu; Fu Gu Zhang; Ke Jin Zhou; Zong He Zhu
... In recent years, due to the change of farming system, delayed the sowing time of rapeseed, and low temperature and drought would affect the sowing quality, seedling growth, and rapeseed yield.Thirty-one rapeseed materials were screened for low-temperature drought resistance; Zayou15 (ZY15) was selected as low temperature and drought resistance, Huiyou48 (HY48) was established as moderate low tempe ...
... BACKGROUND: Heterosis is an important biological phenomenon in which the hybrids exceed the parents in many traits. However, the molecular mechanism underlying seedling heterosis remains unclear. RESULTS: In the present study, we analyzed the leaf transcriptomes of strong hybrids (AM, HM) and weak hybrids (CM, HW) and their parents (A, C, H, M, and W) at two periods. Phenotypically, hybrids had ob ...
... The canola flower midge, Contarinia brassicola Sinclair (Diptera: Cecidomyiidae), is a newly-described species that induces galls on canola, Brassica napus Linnaeus and Brassica rapa Linnaeus (Brassicaceae). Identification of the sex pheromone of C. brassicola is essential to developing monitoring tools to elucidate the geographic range and hosts of this new pest, and the extent to which it threat ...
... Alternative splicing is a general mechanism that regulates gene expression at the post-transcriptional level, which increases the transcriptomic diversity. Oilseed rape (Brassica napus L.), one of the main oil crops worldwide, is prone to secondary dormancy. However, how alternative splicing landscape of oilseed rape seed changes in response to secondary dormancy is unknown. Here, we analyzed twel ...
... Cover crop adoption in conventional no-tillage corn/soybean production systems has been limited due to the lack of specific information regarding the impact of cover crops on cash crop performance. Within a no-till small grain/cover crop-corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] rotation in eastern South Dakota, we evaluated the impact of fall-planted forage oat (Avena sativa L.), hairy ...
Alfonso Allen‐Perkins; Ainhoa Magrach; Matteo Dainese; Lucas A. Garibaldi; David Kleijn; Romina Rader; James R. Reilly; Rachael Winfree; Ola Lundin; Carley M. McGrady; Claire Brittain; David J. Biddinger; Derek R. Artz; Elizabeth Elle; George Hoffman; James D. Ellis; Jaret Daniels; Jason Gibbs; Joshua W. Campbell; Julia Brokaw; Julianna K. Wilson; Keith Mason; Kimiora L. Ward; Knute B. Gundersen; Kyle Bobiwash; Larry Gut; Logan M. Rowe; Natalie K. Boyle; Neal M. Williams; Neelendra K. Joshi; Nikki Rothwell; Robert L. Gillespie; Rufus Isaacs; Shelby J. Fleischer; Stephen S. Peterson; Sujaya Rao; Theresa L. Pitts‐Singer; Thijs Fijen; Virginie Boreux; Maj Rundlöf; Blandina Felipe Viana; Alexandra‐Maria Klein; Henrik G. Smith; Riccardo Bommarco; Luísa G. Carvalheiro; Taylor H. Ricketts; Jaboury Ghazoul; Smitha Krishnan; Faye E. Benjamin; João Loureiro; Sílvia Castro; Nigel E. Raine; Gerard Arjen de Groot; Finbarr G. Horgan; Juliana Hipólito; Guy Smagghe; Ivan Meeus; Maxime Eeraerts; Simon G. Potts; Claire Kremen; Daniel García; Marcos Miñarro; David W. Crowder; Gideon Pisanty; Yael Mandelik; Nicolas J. Vereecken; Nicolas Leclercq; Timothy Weekers; Sandra A. M. Lindstrom; Dara A. Stanley; Carlos Zaragoza‐Trello; Charlie C. Nicholson; Jeroen Scheper; Carlos Rad; Evan A. N. Marks; Lucie Mota; Bryan Danforth; Mia Park; Antônio Diego M. Bezerra; Breno M. Freitas; Rachel E. Mallinger; Fabiana Oliveira da Silva; Bryony Willcox; Davi L. Ramos; Felipe D. da Silva e Silva; Amparo Lázaro; David Alomar; Miguel A. González‐Estévez; Hisatomo Taki; Daniel P. Cariveau; Michael P. D. Garratt; Diego N. Nabaes Jodar; Rebecca I. A. Stewart; Daniel Ariza; Matti Pisman; Elinor M. Lichtenberg; Christof Schüepp; Felix Herzog; Martin H. Entling; Yoko L. Dupont; Charles D. Michener; Gretchen C. Daily; Paul R. Ehrlich; Katherine L. W. Burns; Montserrat Vilà; Andrew Robson; Brad Howlett; Leah Blechschmidt; Frank Jauker; Franziska Schwarzbach; Maike Nesper; Tim Diekötter; Volkmar Wolters; Helena Castro; Hugo Gaspar; Brian A. Nault; Isabelle Badenhausser; Jessica D. Petersen; Teja Tscharntke; Vincent Bretagnolle; D. Susan Willis Chan; Natacha Chacoff; Georg K. S. Andersson; Shalene Jha; Jonathan F. Colville; Ruan Veldtman; Jeferson Coutinho; Felix J. J. A. Bianchi; Louis Sutter; Matthias Albrecht; Philippe Jeanneret; Yi Zou; Anne L. Averill; Agustin Saez; Amber R. Sciligo; Carlos H. Vergara; Elias H. Bloom; Elisabeth Oeller; Ernesto I. Badano; Gregory M. Loeb; Heather Grab; Johan Ekroos; Vesna Gagic; Saul A. Cunningham; Jens Åström; Pablo Cavigliasso; Alejandro Trillo; Alice Classen; Alice L. Mauchline; Ana Montero‐Castaño; Andrew Wilby; Ben A. Woodcock; C. Sheena Sidhu; Ingolf Steffan‐Dewenter; Ioannis N. Vogiatzakis; José M. Herrera; Mark Otieno; Mary W. Gikungu; Sarah J. Cusser; Thomas Nauss; Lovisa Nilsson; Jessica Knapp; Jorge J. Ortega‐Marcos; José A. González; Juliet L. Osborne; Rosalind Blanche; Rosalind F. Shaw; Violeta Hevia; Jane Stout; Anthony D. Arthur; Betina Blochtein; Hajnalka Szentgyorgyi; Jin Li; Margaret M. Mayfield; Michał Woyciechowski; Patrícia Nunes‐Silva; Rosana Halinski de Oliveira; Steve Henry; Benno I. Simmons; Bo Dalsgaard; Katrine Hansen; Tuanjit Sritongchuay; Alison D. O'Reilly; Fermín José Chamorro García; Guiomar Nates Parra; Camila Magalhães Pigozo; Ignasi Bartomeus
Bombyliidae; Brassica napus; Citrullus lanatus; Malus domestica; Syrphidae; Vaccinium corymbosum; crop yield; data collection; databases; ecology; ecosystem services; flowers; fruits; global change; honey; insect pollination; pollinators; Africa; Asia; Caribbean; Europe; Latin America; North America; Pacific Ocean Islands
Abstract:
... Seventy five percent of the world's food crops benefit from insect pollination. Hence, there has been increased interest in how global change drivers impact this critical ecosystem service. Because standardized data on crop pollination are rarely available, we are limited in our capacity to understand the variation in pollination benefits to crop yield, as well as to anticipate changes in this ser ...
... As azo dyes are carcinogenic, toxic, and biodegradable, the harmless treatment of dye wastewater has become increasingly urgent. This study aimed to investigate the decolorization and detoxification effect of a Stenotrophomonas acidaminiphila EFS1 strain on methyl orange (MO), and the application prospect in the biological treatment of dye wastewater. The linear fitting analysis between the time e ...
Brassica napus; canola; equations; irrigation; normal distribution; phenology; reflectance; spectral analysis; temporal variation; time series analysis; unmanned aerial vehicles; vegetation
Abstract:
... Accurate information about phenological stages is essential for canola field management practices such as irrigation, fertilization, and harvesting. Previous studies in canola phenology monitoring focused mainly on the flowering stage, using its apparent structure features and colors. Additional phenological stages have been largely overlooked. The objective of this study was to improve a shape-mo ...
... Many agricultural soils fail to supply sufficient boron (B) and phosphorus (P) to growing plants due to their adsorption, precipitation and fixation phenomena. Moreover, the application of individual B sources without a macronutrient fertilizer is impractical owing to the separate handling and poor distribution in the field. The study aimed to develop and compare the efficiency of organically comp ...
... Pollen beetles (Coleoptera: Nitidulidae) rank among the most important pests of oilseed rape (Brassica napus). For their timely detection in early spring, yellow sticky or water pan traps are used; however, it has been suggested that the addition of chemical lures to attractive visual cues could improve trap efficacy. During the course of field trials in Hungary, we have developed a 3-component sy ...
... Rapeseed (Brassica napus) is an oil crop grown worldwide, making it a key plant species in molecular breeding research. However, the complexity of its polyploid genome increases sequencing costs and reduces sequencing accuracy. Target capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of interest. In this study, 588 r ...
... Brassica napus L is an important oilseed crops grown throughout many parts of the world. It is well adapted to long day photoperiods. Synthetic B. napus was produced through interspecific hybridization between B. rapa L. var Tori-7 and exotic variety of B. oleracea L. var. alboglabra Bailey. The chromosomes of the interspecific hybrids were doubled through the treatments of colchicine. Synthetic B ...
... The 8029AB line is a dominant genic male sterility (DGMS) two-type line in Brassica napus L., which can be used in a three-line approach for the seed production of rapeseed hybrids. Genetic analyses have demonstrated that the sterility of 8029A is controlled by a single dominant nuclear gene (BnMS5ᵉ) interacting with one recessive gene (BnMS5ᶜ). Six pairs of penta-primer amplification refractory m ...