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Citrus maxima; Oriental traditional medicine; carbon dioxide; citrus peels; coumarins; cultivars; flavonoids; liquid chromatography; pummelos; separation; supercritical fluid extraction; tandem mass spectrometry; China
Abstract:
... Five pomelo cultivars (i.e., Citrus grandis cv. Shatianyou, Citrus grandis cv. Guanximiyou, Citrus grandis cv. Yuhuanyou, Citrus grandis cv. Duweiwendanyou and Citrus grandis cv. Liangpingyou) from different origins in China were selected to analyze their components by using supercritical CO₂ fluid extraction coupled with ultra‐high‐performance liquid chromatography‐tandem mass spectrometry. A tot ...
... Histological studies of stem, root and leaf of ovule and embryo cultured haploid and diploid plantlets of Citrus grandis × C. sinensis and Citrus grandis × C. limetta crosses were carried out. Area of xylem and phloem in stem increased gradually with increasing age of explants in both cross combinations; however, it was measured minimum in haploid plantlets (1.95 and 1.62 mm², respectively) as com ...
... Although it is usually latent on citrus, apple, and pear, apple stem grooving virus (ASGV) poses a great risk to many sensitive cultivars. Since severe leaf yellow mottle mosaic (LYMM) symptoms have been observed on Huangjinmiyou (HJY) pummelos (Citrus grandis cv. Huangjinmiyou), a commercial variety that is widely cultivated in South China, high throughput sequencing (HTS) was used to find potent ...
Citrus maxima; atomic force microscopy; calcium; crosslinking; food chemistry; fruit quality; fruits; gelation; juices; lignification; metabolism; pectinesterase; pectins; polygalacturonase; pummelos; sodium; water solubility
Abstract:
... Postharvest granulation is a severe physiological disorder in citrus fruit that may be related to pectin metabolism. This study was designed to analyze the changes in fruit quality and pectin metabolism of pomelo fruit during granulation from expansion, gelation to lignification. Fruit quality showed a decrease starting from the gelation stage. Contents of water-soluble pectin, chelate-soluble pec ...
... Cold extraction methods with ethanol applied to the flavedo of Citrus fruits have been commonly applied for the preparation of several liquors. In order to obtain the extraction optimization and then the best ratio of functional ingredients in the extract, the flavedo of Citrus grandis Osbeck (pummelo) was subjected to a maceration with absolute ethanol at room temperature as well as at 40 °C. The ...
... Electrocatalysis of bioflavonoids in carbon nanomaterials plays an important role in electrochemical sensors for the detection of their content in fruits. In this study, three types of carbon nanomaterials with 1D, 2D, and 3D structures, namely carbon nanotubes (CNTs), graphene oxide (GO), and Ketjen black (KB), were modified onto glassy carbon electrodes for the electrocatalysis of hesperidin and ...
... The incidence of colorectal cancer is rapidly growing in Korea, due to increasingly westernized dietary. We investigated the inhibitory effects of Citrus grandis extracts rich in polymethoxylated flavones in colorectal cancer. CGDF treatment changed the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins and generated ROS. CGDF treatment could promote the release of cytochrome c into t ...
Citrus maxima; acidity; alluvial soils; biochar; calcium; composts; field experimentation; fruit yield; mineral fertilizers; pummelos; river deltas; soil density; soil fertility; soil organic matter; soil pH; subsoil; topsoil
Abstract:
... Soil acidity is an important factor affecting crop productivity in the tropics. The soil of the Vietnamese Mekong Delta has high acidity, deficient levels of exchangeable bases, and low fertility. This study aimed to evaluate soil amendments’ role in improving soil fertility, soil acidity, and pomelo yield cultivated in raised beds. The field trial was carried out in Hau Giang Province consecutive ...
Citrus maxima; agronomy; fertilizers; foliar application; fruit quality; fruit yield; fruits; leaves; magnesium; nutrient content; nutrient uptake; phosphorus; potassium; pummelos; river deltas; soil fertility; zinc
Abstract:
... Foliar application is a popular technique to supply mineral nutrients to crops. It also enhances nutrient use efficiencies, especially under limited soil fertility or restricted environmental conditions. This study aimed to evaluate the influence of foliar application of phosphorus (P) with potassium (K), magnesium (Mg), and zinc (Zn) on the nutrient uptake, yield, and fruit quality of pomelo. The ...
Arabidopsis; Citrus maxima; biosynthesis; flowering; fruit juices; fruit texture; gene expression; genes; lignin; luciferase; mesocarp; postharvest storage; pummelos; taste; transcription (genetics); transcription factors; transcriptional activation; two hybrid system techniques; yeasts
Abstract:
... Overaccumulation of lignin (a physiological disorder known as granulation) often occurs during fruit ripening and postharvest storage in pomelo (Citrus grandis). It causes an unpleasant fruit texture and taste. Previous studies have shown that lignin metabolism is closely associated with the process of juice sacs granulation. At present, the underlying transcriptional regulatory mechanisms remain ...
... MAIN CONCLUSION: CgVPE1 is important in the differentiation of TE cells in C. grandis ‘Tomentosa’ fruits as it may directly affects secondary cell wall construction while participating in PCD. The vacuolar processing enzyme (VPE) plays an important role in both developmental and environmentally inducible programmed cell death (PCD); it was originally identified as a cysteine protease localized in ...
... Vesicle granulation is a common internal physiological disorder in citrus fruit, associated with excessive consumption of sugar and acid. Aside from cell wall metabolism, it remains largely unexplored how the sugar and acid metabolic pathways altered as vesicle granulation progressed. The present study performed systematic analysis of sugar- and acid-related genes and metabolites in vesicles durin ...
... In this study, the effect of pomelo juice foam composition on the foam properties was investigated for the production of foam mat‐dried pomelo juice powder. The pomelo juice foam was prepared using maltodextrin (MD), glycerol monostearate (GMS), and carboxymethyl cellulose (CMC) at various concentrations in the range of 2–8%, 0.5–2%, and 5–10%, respectively. A face‐centered central composite desig ...
... This work is focused on the modification of insoluble dietary fiber from pomelo (Citrus grandis) peel (PP‐IDF) by ball milling. Ball milling intensified water and oil holding capacity and water swelling capacity by 43.7%, 65.1%, and 107.5%, respectively. Besides, 97.1% of taurocholic acid, 45.3% of glucose, and 9.3 μg/mg of NO₂⁻ were bound by milled insoluble dietary fibers (IDFs). The oil‐in‐wate ...
... Little information is available on the physiological and molecular adaptations of plant roots to long-term copper (Cu) excess revealed by combined analysis of physiology, widely targeted metabolome and RNA-Seq. ‘Shatian’ pummelo [Citrus grandis (L.) Osbeck] seedlings were supplied six times weekly with nutrient solution at a Cu concentration of 400 (excessive Cu) or 0.5 (control) μM for six months ...
... In this study, the main flavanones, polymethoxyflavones, anthocyanins and furanocoumarins were evaluated in two blood grapefruits (CQP 31 and CQP 32), in their juice, peel, and leaves, by means of HPLC-DAD analysis and identified by HPLC-MS-MS. They are compared with the contents of these secondary metabolites in three commercial grapefruits: Star Ruby, Marsh, and Duncan; a blood orange, cv. Sangu ...
... The Citrus genus is a good source of dietary flavonoids, which have many health benefits. As a representative citrus fruit, the flavonoids composition in Shatianyu (Citrus grandis L. Osbeck) pulp remains to be investigated. In the present study, 11 flavonoids were isolated and identified from Shatianyu pulp flavonoid extracts (SPFEs). Among them, 4 flavonoids were previously undescribed and 2 flav ...
... The extraction of bioactive compounds, including essential oils and flavonoids, using organic solvents is a significant environmental concern. In this work, waste C. grandis peel was the ingredient used to extract essential oil and naringin by conducting a supercritical CO₂ technique with a two stage process. In the first stage, the extraction with only supercritical CO₂ solvent showed a significa ...
... Carotenoids are indispensable for human health, and citrus fruit are a crucial source of dietary carotenoids. Bagging, an important orchard practice to enhance fruit economic value, is widely used in many horticultural crops, including citrus fruit. The bagged ‘Majiayou’ pomelo (Citrus grandis) produces vivid deeper red pulp, a fantastic agronomic trait, but the underlying molecular regulatory mec ...
... BACKGROUND: Many citrus orchards of south China suffer from soil acidification, which induces aluminum (Al) toxicity. The Al-immobilization in vivo is crucial for Al detoxification. However, the distribution and translocation of excess Al in citrus species are not well understood. RESULTS: The seedlings of ‘Xuegan’ [Citrus sinensis (L.) Osbeck] and ‘Shatianyou’ [Citrus grandis (L.) Osbeck], that d ...
... Previous results indicated that the flavonoid profiles might have varietal differences in pomelo, but detailed information is unknown. We previously isolated 4 new flavonoids, cigranoside C, D, E, F, in Citrus grandis Shatianyu pulp. However, their distribution in different pomelo cultivars remains to be explored. Therefore, the flavonoid profiles and in vitro bioactivity of the pulp from 5 pomelo ...
... The characteristic parameters of Citrus grandis var. Longanyou canopies are important when measuring yield and spraying pesticides. However, the feasibility of the canopy reconstruction method based on point clouds has not been confirmed with these canopies. Therefore, LiDAR point cloud data for C. grandis var. Longanyou were obtained to facilitate the management of groves of this species. Then, a ...
... Caspase-3 is the crucial executor caspase of apoptosis in mammalian cells, which is essential for chromatin condensation and DNA fragmentation. Although plants have no caspase-3 homologs, PBA1 acts as a plant caspase-3-like enzyme in plant programmed cell death (PCD). PCD occurs during the formation of secretory cavities in Citrus fruits; hence, secretory cavities could be utilized as a new cell b ...
... Citrus grandis “Tomentosa” (“Huajuhong”) is a famous Traditional Chinese Medicine. In this study, a total of 18 jumonji C (JMJC) domain-containing proteins were identified from C. grandis. The 18 CgJMJCs were unevenly located on six chromosomes of C. grandis. Phylogenetic analysis revealed that they could be classified into five groups, namely KDM3, KDM4, KDM5, JMJC, and JMJD6. The domain structur ...
... Cuticular wax covers the surface of fleshy fruit and plays a protective role in fruit development and postharvest storage, including reducing fruit water loss, resisting biotic and abiotic stress and affecting fruit glossiness. The β-ketoacyl-CoA synthase (KCS) is the rate-limiting enzyme of very long chain fatty acids (VLCFAs) synthesis, which provides precursors for the synthesis of cuticular wa ...
Citrus maxima; cDNA libraries; cultivars; disease resistance; drought; fluorescence; fruit growing; gibberellins; greening disease; indole acetic acid; industry; pummelos; resistance genes; salicylic acid; two hybrid system techniques
Abstract:
... Huanglongbing (HLB) disease, also known as citrus greening, is a severe threat for citrus fruit cultivation industry. Till date, neither any effective radical method to control HLB nor HLB resistant cultivar is developed. The aim of this study was to provide clues for unraveling the molecular regulatory mechanism of citrus in response to HLB, and to provide reference gene for breeding of HLB resis ...
... Quality of fruit and the subsequent eating attribute is closely linked factors that have a significant effect on marketability and have always been correlated with harvest maturity. In the present investigation, the main focus was given on the development of an inexpensive and rapid tool for identification of appropriate plucking time in the field through colour chart and ANN modeling. The effect ...
... Rare earth elements (REEs) can affect the growth and development of plants. However, few studies have been carried out on the effects of REEs on citrus seedlings. In this study, the growth parameters, toxicity symptoms, chlorophyll content, and La content of three citrus rootstocks are analyzed under different concentrations of La, a representative REE. The results show that the growth of citrus r ...
... Huyou (Citrus grandis (L.) Changshan‐huyou) from ChangShan in Zhejiang, China, is one of the most popular fruits cultivated and consumed worldwide. However, there are no reports on improvement of the edible value through fermentation processing using lactic acid bacteria (LAB). In this study, effects of pretreatment of Huyou juice with dimethyl dicarbonate (DMDC) on quality attributes of the produ ...
... In the present study, the fruit physiology and sugar-acid ratio of 24 pomelo cultivars grown in ten different locations of the subtropical region of China were measured. The contents of soluble sugars and organic acids were quantified using high-performance (HPLC-MS) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS), respectively. The results revealed that the physiological a ...
Citrus maxima; Citrus sinensis; agronomy; biomass; biosynthesis; carbon dioxide; electron transport chain; homeostasis; nitrogen; nitrogen content; nutrient balance; nutrient solutions; nutrient use efficiency; photosynthetic electron transport; plant growth; thylakoids
Abstract:
... Limited data are available on the physiological responses of Citrus to nitrogen (N) deficiency. ‘Xuegan’ (Citrus sinensis (L.) Osbeck) and ‘Shantian pummelo’ (Citrus grandis (L.) Osbeck) seedlings were fertilized with nutrient solution at a N concentration of 0, 5, 10, 15 or 20 mM for 10 weeks. N deficiency decreased N uptake and N concentration in leaves, stems and roots and disturbed nutrient ba ...
... This study aims to determine carotenoids in pomelo leaves (Citrus grandis Osbeck), a rich source of nutrients and phytochemicals, by high-performance liquid chromatography-mass spectrometry and prepare carotenoid nanoemulsions for the study of its inhibitory mechanism on melanoma cells A375. Fourteen carotenoids were separated within 27 min by using a YMC-C30 column and a gradient mobile phase of ...
... Root system plays a crucial role in plant growth and development by uptake of soil nutrients, which is affected by intensive use of NPK fertilizer. However, it is unknown how integrated nutrient management (INM) could affect the root growth and its nutrient uptake in the red soils of southern China. For this, the impacts of different INM practices on root morphological traits and root nutrient upt ...
... The naringinase enzyme was isolated from Citrus macroptera peel and pomace using Aspergillus niger for immobilization and debittering of juice. A comparative study of the effect of debittering using immobilized enzyme and resin on the physicochemical and phytochemical properties of the juice were carried out. The optimum hydrolytic conditions and Michaelis constant for an immobilized enzyme were o ...
... Granulation is a physiological disorder of juice sacs in citrus fruits, which develops through secondary cell wall formation. However, the synergistic changes in the cytoplasm of juice sac cells remain largely unknown. This study investigated the dynamic ultrastructure of juice sacs of “Guanxi” pummelo fruits by transmission electron microscopy and determined their cell wall material, soluble suga ...
Citrus maxima; Citrus paradisi; adsorption; arabinose; chemical structure; cholesterol; cultivars; dietary fiber; galactose; glucose; grapefruits; nitrites; oils; pulp; pummelos; water
Abstract:
... The contents and chemical structure of dietary fiber (DF), along with the physicochemical (water retention, water swelling and oil adsorption capacity) and functional properties (glucose adsorption, cholesterol adsorption and nitrite ion adsorption capacity) of insoluble and soluble dietary fiber (IDF, SDF) from the pulp of 5 pummelo (Citrus grandis L. Osbeck) and one grapefruit (Citrus paradisi M ...
... Pomelo (Citrus grandis), an important fruit crop grown in tropical and subtropical areas, is cultivated mainly in Asian countries. The dominant pigment in pomelo leaves, chlorophyll, has been reported to possess many biological activities such as antioxidant, anti-inflammation and anticancer. The objectives of this study were to determine chlorophylls in Pomelo leaves by high-performance liquid ch ...
... 1,2-Rhamnosyltransferase (1,2RhaT) catalyzes the final step of production of flavanone neohesperidoside (FNH) that is responsible for the primary bitter taste of citrus fruits. In this study, species-specific flavonoid profiles were determined in 87 Citrus accessions by identifying eight main flavanone glycosides (FGs). Accumulation of FNHs was completely correlated to the presence of the 1,2RhaT ...
Citrus maxima; citrus fruits; equipment; fruit quality; models; postharvest storage; support vector machines; wavelengths
Abstract:
... Citrus fruit granulation is a major physical disorder during late maturity and post-harvest storage, and it greatly undermines the quality of fruit. Currently, there is still a lack of rapid and nondestructive methods to detect citrus granulation. This study proposes a nondestructive granulation detecting method based on deep learning. Different models were established with the input of preprocess ...
Citrus maxima; air; chlorophyll; cultivars; flowering; flowers; fruits; gene expression; genes; leaves; loci; pummelos; stomatal conductance; total nitrogen; water stress
Abstract:
... One of the major problems in the fruit production of citrus, including pummelo (Citrus grandis) is controlling flowering induction. Water stress is known to be related to flowering induction via physiological responses related to the flowering gene. However, reports on the mechanisms underlying floral induction by water stress in pummelo are limited. Thus, this study aimed to determine the physiol ...
... Limonoids, a group of highly oxygenated bioactive triterpenoid components, are abundant in citrus. In the present study, a comprehensive metabolomic analysis of limonoids was performed among the flavedo, albedo, segment membrane, juice vesicles, and seeds of pummelo by using Quadrupole-Orbitrap UHPLC-MS/MS. As a result, 21 limonoids were identified in the five tissues and their MS/MS fragmentation ...
... Deposition of both lignin and cellulose accompanied by juice sac granulation is widespread in harvested citrus fruit. Hence, measures to suppress postharvest granulation of ‘Majiayou’ pummelo is of great importance. The fruit was treated with 1.5% chitosan and then stored at room temperature (20 ± 2 °C) for 150 d. As compared to the control fruits, chitosan coating significantly suppressed granula ...
... Citrus fruits exhibit a high level of different phytoconstituents, of which the changes in the different parts of the fruit during ripening have not been thoroughly studied yet. Thus, in this study, we have investigated how different parts of pomelo fruit (Citrus grandis L.) are modified throughout the development of two consecutive growing seasons. In detail, the main phytochemical compounds, suc ...
... The antioxidant activity (1,1‐diphenyl‐2‐picrylhydrazyl (DPPH)) values varied between 9.71 (methanol:water, 60:40 v/v) and 13.30% (methanol:water, 50:50 v/v) and total phenol contents ranged between 12.43 (ethanol:water, 60:40 v/v) and 22.91 mg GAE/kg (methanol:water, 50:50 v/v). In general, pomelo fruit carpels (PFCs or the carpels with juicy vesicles) extracts obtained using methanol:water solut ...
Citrus maxima; Oriental traditional medicine; Western blotting; antitussive agents; cultivars; drug development; enzyme-linked immunosorbent assay; immunomodulators; inducible nitric oxide synthase; interleukin-6; macrophages; mitogen-activated protein kinase; nitric oxide; polysaccharides; reverse transcriptase polymerase chain reaction; secretion; signal transduction; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Citrus grandis ‘Tomentosa’ which is a special Citrus cultivar, has been employed as cough suppressant and expectorant in traditional Chinese medicine for thousands of years. The aim of this study is to investigate the immunomodulatory role of an acidic polysaccharide (designated as CGTP-AP) purified from C. grandis ‘Tomentosa’. CGTP-AP showed effective immune activation in RAW264.7 macrophages at ...
... The indiscriminate use of synthetic insecticides for the control of harmful mosquitoes has been fostering menaces like resistance development, toxic hazards to non-target organisms etc. Here, studies are made to prepare a synergistic mixture comprising of low concentration of essential oils of Allium sativum (bulbs), Ocimum sanctum (leaves), Citrus grandis (leaves and peels) against Culex quinquef ...
... Juice sac granulation is a serious problem in pummelo (Citrus grandis L. Osbeck) fruits during postharvest storage owing to various abiotic stress conditions, which greatly reduces their attractiveness and commercial value. Hence, measures to alleviate the postharvest juice sac granulation of pummelo are of great importance. The current study was designed to investigate the effects of 1.5 % chitos ...
Betaflexiviridae; Citrus maxima; Cytorhabdovirus; RNA; Trifolium pratense; amino acid sequences; coat proteins; genome; high-throughput nucleotide sequencing; new genus; nucleotides; open reading frames; phylogeny; polyproteins; pummelos; tail; trees; virology; viruses; China
Abstract:
... A novel plant virus with a positive single-stranded (+ss) RNA genome was detected in Taibei pomelo (Citrus grandis (L.) Osbeck cv. Taibeiyou) in China by high-throughput sequencing (HTS). Tentatively named “citrus yellow spot virus” (CiYSV), it has 8,061 nucleotides (nt) excluding the poly(A) tail and contains three open reading frames (ORFs). ORF1 is predicted to encode a replicase polyprotein (R ...
... Diversity in fruit genetic resources in Nepal is contributed by wild, indigenous and exotic sources. This study was carried out to bring together the available fruit species and cultivars at various stations of Department of Agriculture (DoA), Nepal Agricultural Research Council (NARC), Agriculture and Forest University (AFU) and private farms until the Fiscal Year 2017/2018. Altogather there were ...
... Pomelo fruit juice is concentrated using two different techniques, i.e. block freeze concentration (BFC) and thermal concentration (TC), and both concentrates were spray dried separately to observe the effects of two different concentration techniques on the physicochemical properties of the spray dried powder. BFC juice had comparatively better values of retention of acidity, colour, vitamin C, D ...