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... Tannins extracted from the green bark of each of two Chinese, Japanese, and American chestnut trees were assayed in a protein-binding test. Four levels of tannins were added to a buffered, minimal growth medium, and a standard, virulent strain of the chestnut blight fungus was grown. There were only slight differences in protein binding between the extracts from different species. Fungal growth wa ...
restriction fragment length polymorphism; gel electrophoresis; surveys; fungi; reverse transcription; viruses; Castanea; double-stranded RNA; screening; Cryphonectria parasitica; Cryphonectria hypovirus 1; biological control
Abstract:
... In Macedonia, hypoviruses in the chestnut blight fungus, Cryphonectria parasitica, were first identified in 1995, more than 20 years after the discovery of chestnut blight. Between 1995 and 2000, 488 isolates of the fungus were collected from 19 sites throughout Macedonia. These isolates were screened for hypoviruses by extraction of double-stranded RNA (dsRNA) and electrophoresis on agarose gels. ...
Castanea; Cryphonectria parasitica; double-stranded RNA; fungi; sexual reproduction; virulence; Azores; Portugal
Abstract:
... Cryphonectria parasitica, the causal agent of chestnut blight, has been responsible for the decline in chestnut in Portugal for the last two decades. In order to improve understanding of C. parasitica diversity, a total of 617 isolates from all affected chestnut-growing areas in continental Portugal, Madeira and the Azores archipelagos were studied. Nine vegetative compatibility (vc) types were id ...
... In order to improve understanding of its diversity, 338 isolates of Cryphonectria parasitica, the causal agent of chestnut blight, were sampled from 10 chestnut populations throughout chestnut-growing coastal and continental areas of Croatia. Eighteen vegetative compatibility (VC) types were identified. The VC type EU-1 was the most widespread, comprising 42·9% of the isolates, followed by EU-2 (2 ...
... Three coppiced chestnut stands near Florence (Tuscany, Italy) were monitored for 15 years. Presence of blight, damage, evaluation of different types of cankers and their relationship with stand structure were assessed. Disease incidence ranged from 67% to 99% of infected shoots, and mortality caused by blight was between 6% and 12%. Canker types were defined in the study sites; healing and healed ...
... We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuating hypovirus CHV1-EP713. ...
... A comprehensive study of the population biology of Cryphonectria parasitica, the causal agent of chestnut blight, is required to understand the spread of the epidemic in Europe and its natural regulation by the Cryphonectria hypovirus-1 (CHV-1). With this objective in mind, the diversity in vegetative compatibility (vc) types and mating types of C. parasitica populations was assessed in 43 chestnu ...
... Cyclophilins are peptidyl-prolyl cis-trans isomerases that are highly conserved throughout eukaryotes and are the cellular target of the immunosuppressive drug cyclosporin A (CsA). We cloned cyp1, a cyclophilin A-encoding gene in the phytopathogenic fungus Cryphonectria parasitica, and showed that this gene was downregulated following infection by a virulence-attenuating hypovirus. The function of ...
... We investigated variation in virulence of Cryphonectria hypovirus 1 (CHV-1) to the chestnut blight fungus, Cryphonectria parasitica, in Macedonia by inoculating chestnut stems in the field. We inoculated trees with two isolates of C. parasitica, each infected with one of five isolates of CHV-1, four of which were the same for both fungal isolates. Two virus isolates, [Sk28] and [Sk47], were signif ...
... The gene CpSte11 of Cryphonectria parasitica, which encodes a yeast Ste11 homologue, was cloned and characterized. Gene replacement analysis revealed a high frequency of CpSte11 null mutants. When compared with the wild‐type parent strain, CpSte11 null mutants showed no difference in terms of growth rate or pigmentation. However, CpSte11 null mutants showed a marked decrease in both the number and ...
... Chestnut blight fungus, Cryphonectria parasitica, and its hypovirus present a useful model system for investigating the mechanisms of hypoviral infection. To identify gene products associated with fungal pathogenicity and hypoviral regulation, we attempted a proteomic analysis of the virus-free EP155/2 strain and its isogenic virus-infected UEP1 strain in response to tannic acid (TA), which is abu ...
... Kex2-silenced strains of Cryphonectria parasitica, the ascomycete causal agent of chestnut blight, show a significant reduction in virulence, reduced sexual and asexual sporulation and reductions in mating and fertility. Due to this and the known involvement of Kex2 in the processing of important proproteins in other systems, we searched the whole C. parasitica genome for putative Kex2 substrates. ...
Castanea; Cryphonectria parasitica; Curculio elephas; Cydia; biogeography; chestnuts; genetic markers; geographical distribution; insects; pest management; pests; species diversity; Greece
Abstract:
... Greece has been traditionally considered as a chestnut-producing country; yet, chestnut production has experienced a dramatic decline in the last decades, mainly due to chestnut blight Cryphonectria parasitica and chestnut feeding pests. Though chestnut blight is partially under control, little attention has been granted on chestnut feeding pests in Greece. Two species are described as major pests ...
Castanea; Cryphonectria parasitica; biological control; coppicing; forests; fungi; horticulture; human resources; inoculum; orchards; silvicultural practices; tree age; viruses; Greece
Abstract:
... Since 1963 when chestnut blight was recorded for the first time in Greece, the disease has spread into all 29 chestnut growing prefectures. As a result the national, annual chestnut production was reduced from 18,000 tons in the 1960s to 11,000 tons in 2005. A project for the application of biological control on a nationwide scale was funded and implemented during the period 2007-2009. Hypovirulen ...
... Chestnut blight is controlled in Europe by using Cryphonectria hypovirus CHV1, a non-encapsulated RNA virus. The chestnut blight fungus, Cryphonectria parasitica, is weakened by the virus, and healing tissue growth occurs in the host tree. Transmission of this cytoplasmic hypovirus is restricted by the incompatibility system of the fungus, so that the hypovirus can be transmitted only between isol ...
... The biological function(s) of the cpkk1, cpkk2 and cpkk3 genes, encoding the three mitogen‐activated protein kinase kinases (MAP2Ks) of Cryphonectria parasitica, the causal agent of chestnut blight, were examined through knockout strains. Cpkk1, the Mkk1 orthologue, acts in a phosphorylation cascade essential for cell integrity; Cpkk2 is the Ste7 orthologue involved in the pheromone response pathw ...
... The ascomycete fungus, Cryphonectria parasitica (Murr.) Barr, is an important fungal pathogen of chestnut in Europe and North America. The fungus grows into the bark up to the cambium, causing typical diffuse cancers and the death of distal parts of the trunks or branches. The pathogen kills the infected tree branches and the rapid death of the entire tree take place which is causing high environm ...
... Since 1976, when the first occurrence of chestnut blight caused by Cryphonectria parasitica was observed in the locality of Duchonka, West Slovakia, the disease has spread to 43 new areas. Only areas and sites with a lower number of chestnut trees are still without the disease. In this study we have analyzed records on the first occurrence of chestnut blight in different chestnut localities in com ...
... Hypovirulent strains of the chestnut blight fungus Cryphonectria parasitica have not been registered in the autonomous region of Castilla y León (Spain), except in the province of León. In this laboratory-based study, we analyzed the rates of horizontal transmission of hypovirus CHV1 subtype F1, isolated from chestnut stands in León. We tested the conversion capacity of the six vegetative compatib ...
... Chestnut blight caused by Cryphonectria parasitica has been one of the major reasons for the decline of chestnut cultivation in Greece over the last 50 years. A previous detailed study of the vc types of the fungus has revealed only four vc types in the entire country, those of EU‐1, EU‐2 and EU‐10 with the dominant being EU‐12 counting for 88% of the isolates. As the loss in orchard trees reached ...
... Recently, there has been an increasing interest among researchers in using combinations of biological control agents to exploit potential synergistic effects among them. In the present study, there were investigated commercially a