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Castanea; Cryphonectria parasitica; apples; autophagosomes; autophagy; blight; cysteine proteinases; genes; hyphae; mutants; phenotype; plant pathology; sporulation; stress tolerance; two hybrid system techniques; virulence; virulent strains; yeasts; Republic of the Congo
Abstract:
... Autophagy is an evolutionarily ancient process wherein cells are able to break down intracellular contents to support normal physiology and development. Autophagosome formation is regulated by several different proteins, including the key cysteine protease Atg4. The contribution of Atg4 protein in the pathogenic fungus Cryphonectria parasitica, which causes blight in chestnut plants, has not been ...
... Cryphonectria parasitica (Murrill) M.E. Barr (Sordariomycetes, Valsaceae) is the causal agent of chestnut blight. This disease is a major concern for chestnut cultivation in Europe. The fungus colonizes vascular tissues and evolves generating cankers causing severe dieback and the death of the tree. Excised and debarked well-lignified shoots of 28 C. sativa genotypes (assay A) and of 10 progenies ...
... Laccase3 is an important virulence factor of the fungus Cryphonectria parasitica. Laccase3 gene (lac3) transcription is induced by tannic acid, a group of phenolic compounds found in chestnut trees, and its induction is regulated by the hypovirus CHV1 infection. CpHsp24, a small heat shock protein gene of C. parasitica, plays a determinative role in stress adaptation and pathogen virulence. Having ...
Thomas E. Witte; Sam Shields; Graham W. Heberlig; Mike G. Darnowski; Anatoly Belov; Amanda Sproule; Christopher N. Boddy; David P. Overy; Myron L. Smith
... Vegetative incompatibility (VI) is a form of non-self allorecognition in filamentous fungi that restricts conspecific hyphal fusion and the formation of heterokaryons. In the chestnut pathogenic fungus, Cryphonectria parasitica, VI is controlled by six vic loci and has been of particular interest because it impedes the spread of hypoviruses and thus biocontrol strategies. We use nuclear magnetic r ...
... Chestnut blight caused by Cryphonectria parasitica is a severe disease worldwide affecting chestnut trees. One pathway for its long-range transmission is grafting by using non-symptomatic scions. The effects of five disinfectants, 80% ethanol, 2.5% NaOCl, 5% H₂O₂, 2% copper sulphate and Lysol® (Brand III), were evaulated on artificially inoculated scions of 15–20 cm with a spore suspension (1 × 10 ...
... Ambrosia beetles exhibit broad host ranges but a narrow preference based on the condition of the host. Tissues infected by pathogens or containing ethanol can facilitate attacks by ambrosia beetles, although it still remains unclear how these factors interact. The present study aimed to examine how (i) chestnut logs infected with the fungal pathogen Cryphonectria parasitica and treated with ethano ...
... BACKGROUND: Chestnut blight, caused by Cryphonectria parasitica, is controlled in many European countries by the naturally occurring mycovirus Cryphonectria hypovirus 1 (CHV‐1). During surveys of recently identified chestnut blight outbreak in England, CHV‐1 was detected in several individuals of the pathogen isolated from affected trees. We investigated two of these CHV‐1‐infected isolates (L‐6 a ...
... Chestnut blight, Cryphonectria parasitica (Murrill) Barr, was reported in Portugal in 1989. The disease rapidly spread within the chestnut region of the Trás-os-Montes (the Northeast of Portugal). Eradication and mechanical/burning exclusion failed to control the disease and natural hypovirulence is still absent or of very low incidence. The introduction of human-assisted therapeutic hypovirulence ...
... The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates ...
... We identified a protein spot showing downregulation in the presence of Cryphonectria hypovirus 1 and tannic acid supplementation as a septin subunit with the highest homology to the Aspergillus nidulans aspA gene, an ortholog of the Saccharomyces cerevisiae Cdc11 gene. To analyze the functional role of this septin component (CpSep1), we constructed its null mutant and obtained a total of eight CpS ...
... The development and validation of a real-time PCR method for the detection of Cryphonectria parasitica in bark tissues is described. The selected region in the genome was a fragment of the internal transcribed spacer region. The DNA extraction and PCR conditions were optimized to be routinely applicable to fresh and dried bark. The sensitivity of the assay allowed the detection of 2 fg of genomic ...
... The diversity of the fungal community associated with adults of Dryocosmus kuriphilus following emergence was examined using HTS analysis. Ascomycota dominated the fungal core-biome community. The functional guilds of the 90 taxa forming the core-biome were assessed, demonstrating three main groups: saprotrophs, plant pathogens and entomopathogens. Twenty-nine OTUs out of 90 were resolved to speci ...
... Transmissible hypovirulence associated with Cryphonectria hypovirus 1 (CHV1) has been used for biological control of chestnut blight, devastating disease of chestnut caused by the fungus Cryphonectria parasitica. The main aims of this study were to provide molecular characterization of CHV1 from Croatia and Slovenia and to reveal its genetic variability, phylogeny, and diversification of populatio ...
Jesús Barrena-González; Javier Lozano-Parra; Alberto Alfonso-Torreño; Carlos Lozano-Fondón; Mohamed Amine Abdennour; Artemi Cerdà; Manuel Pulido-Fernández
... Chestnut cultivation is a type of traditional centennial exploitation in southern areas of Extremadura. Currently, chestnut is in danger of extinction due to the effects of climate change, some diseases (e.g. Cryphonectria parasitica) and land mismanagement. The high temporal variability of rainfall leads to soil erosion in chestnut cultivation. New forms of management are proposed to try, among o ...
... The diversity of vegetative compatibility (vc) types of chestnut blight fungus, Cryphonectria parasitica, has been reported to be very low in all of Turkey, except in the eastern Black Sea Region. However, risk for increase in vc type diversity has been pointed out regarding the emergence of new vc types as recombinants during sexual reproduction. In this study, the potential increase in the diver ...
... The vacuolar H⁺-ATPases (V-ATPases) are conserved ATP-dependent proton pumps that acidify intracellular compartments in eukaryotic cells. The role of Cpvma1, a V-ATPase catalytic subunit A of Cryphonectria parasitica, was investigated by generating cpvma1-overexpressing and cpvma1-silenced strains. The mutant strains were evaluated for phenotypic characteristics, V-ATPase activity, response to ele ...
... Biotic stress caused by virus infections induces epigenetic changes in infected plants and animals, but this is the first report on methylation pattern changes in a fungus after mycovirus infection. As a model pathosystem for mycovirus-host interactions, we used Cryphonectria hypovirus 1 (CHV1) and its host fungus Cryphonectria parasitica, in which deregulation of methylation cycle enzymes upon vi ...
Castanea; Cossus; Cryphonectria parasitica; Populus; bark; branches; control methods; equipment; flight; fruit trees; groves; imagos; insecticides; larvae; moths; orchards; oviposition; pests; population density; seasonal variation; spraying; towns; trapping; Turkey (country)
Abstract:
... Goat moth, Cossus cossus L. (Lepidoptera: Cossidae), is a polyphagous pest of many fruit trees, poplar, park, and garden trees. Despite being known as secondary pest, goat moth density has recently increased in chestnut orchards in Turkey. Controlling this pest is very hard because larvae live inside trunks and branches and periods of moth flight and oviposition are very long. Intensive, direct sp ...
... Cryphonectria parasitica is a phytopathogenic fungus introduced from Eastern Asia to North America and to Europe, where it causes chestnut blight, a devastating disease of chestnut trees. The disease can be biologically controlled utilising the mycovirus Cryphonectria hypovirus 1 (CHV1), which changes the physiology of the host, reducing its virulence towards chestnut. We measured fungal growth in ...
... Biological control with Cryphonectria hypovirus CHV1 of the chestnut blight, caused by the fungus Cryphonectria parasitica, has reduced the impact of the disease in Europe. The virus reduces the virulence of the fungus so that it causes non-lethal cankers, thus enabling the chestnut trees to overcome the disease. The virus can be transmitted horizontally by hyphal anastomosis or vertically to the ...
... AIMS: This study aimed to develop a new formulation of Cryphonectria parasitica hypovirulent mycelium suitable for inoculations of tall trees from the ground. Cryphonectria parasitica hypovirulent strains are widely used for biological control of chestnut blight. However, it is often inconsistent and ineffective not only for biological reasons but also because the current manual application of hyp ...
... Chestnut blight is caused by the fungus Cryphonectria parasitica. As one of the most ecologically important diseases of Castanea spp., C. parasitica can rapidly kill trees. In Europe, mitigation of disease severity took place spontaneously through colonization of C. parasitica by mycoviruses, which reduced the virulence of the fungus. In the framework of a survey, 138 C. parasitica isolates were i ...
... Cryphonectria parasitica, an ascomycete fungus, is the causal agent of chestnut blight. This highly destructive disease of chestnut trees causes significant losses, and is therefore a regulated pathogen in Europe. Existing methods for the detection of C. parasitica include morphological identification following culturing, or PCR; however, these are time‐consuming resulting in delays to diagnosis. ...
... Castanea is a hardwood forest genus of considerable agro-economic importance for both timber and nut production. Chestnuts are one of the most significant nut crops in the temperate zone. However, this species is threatened by pollution, social factors, economical changes, and two major fungal diseases: ink disease (Phytophthora spp.), and chestnut blight canker (Cryphonectria parasitica). Similar ...
... BACKGROUND: Chestnut blight, caused by the fungus Cryphonectria parasitica, is a severe chestnut disease that can be controlled with naturally occurring hypoviruses in many areas of Europe. The aim of this research was to measure the effect of different Cryphonectria hypovirus 1 (CHV1) strains on the growth of the fungal host and select strains that could potentially be used for human‐mediated bio ...
... After the first introduction of chestnut gall wasp, Dryocosmus kuriphilus, in Italy, it has spread rapidly in several other European countries, probably largely due to trade in chestnut plants. Despite the measures taken to prevent further spread of this pest to other countries, D. kuriphilus was detected in Greece for the first time in 2014. However, surveys have shown that the pest is still of v ...
Castanea; Cryphonectria parasitica; evolution; fungi; genotype; global change; pathogens; phenotype; temperature; Asia; France
Abstract:
... Range‐expanding species are expected to gain an increasing importance in the context of global change. They provide a great opportunity to study contemporary evolutionary changes and to unravel the mechanisms of evolution. Cryphonectria parasitica, the causal agent of chestnut blight, originating from Asia, has been spread since the beginning of the 20th century into different continents. We took ...
... Hypovirulent strains of the chestnut blight fungus Cryphonectria parasitica have not been registered in the autonomous region of Castilla y León (Spain), except in the province of León. In this laboratory-based study, we analyzed the rates of horizontal transmission of hypovirus CHV1 subtype F1, isolated from chestnut stands in León. We tested the conversion capacity of the six vegetative compatib ...
Castanea; Cryphonectria parasitica; Hypovirus; biological control; biological control agents; fungi; genetic markers; mycoviruses; sequence analysis; sporulation; tree diseases; virulence; Switzerland
Abstract:
... Chestnut blight, caused by the invasive fungus Cryphonectria parasitica, is one of the few tree diseases in Europe for which biological control is possible. The fungus may be infected by a mycovirus that reduces its virulence and sporulation capacity. In this study, we used molecular markers to assess the establishment and dissemination of the Cryphonectria hypovirus (CHV1) after its release as a ...
... Whenever a new invasive forest pathogen appears, resources are put into developing countermeasures such as breeding for resistance in the affected host species and/or by developing fungicide/biocontrol treatments. In most cases, little effort is placed into analysing the likelihood of those measures being implemented. Studying the reasoning behind management reactions of forest managers whose fore ...
... Chestnut blight is controlled in Europe by using Cryphonectria hypovirus CHV1, a non-encapsulated RNA virus. The chestnut blight fungus, Cryphonectria parasitica, is weakened by the virus, and healing tissue growth occurs in the host tree. Transmission of this cytoplasmic hypovirus is restricted by the incompatibility system of the fungus, so that the hypovirus can be transmitted only between isol ...
... Chestnut blight caused by Cryphonectria parasitica has been one of the major reasons for the decline of chestnut cultivation in Greece over the last 50 years. A previous detailed study of the vc types of the fungus has revealed only four vc types in the entire country, those of EU‐1, EU‐2 and EU‐10 with the dominant being EU‐12 counting for 88% of the isolates. As the loss in orchard trees reached ...
... The biological function(s) of the cpkk1, cpkk2 and cpkk3 genes, encoding the three mitogen‐activated protein kinase kinases (MAP2Ks) of Cryphonectria parasitica, the causal agent of chestnut blight, were examined through knockout strains. Cpkk1, the Mkk1 orthologue, acts in a phosphorylation cascade essential for cell integrity; Cpkk2 is the Ste7 orthologue involved in the pheromone response pathw ...
... The ascomycete fungus, Cryphonectria parasitica (Murr.) Barr, is an important fungal pathogen of chestnut in Europe and North America. The fungus grows into the bark up to the cambium, causing typical diffuse cancers and the death of distal parts of the trunks or branches. The pathogen kills the infected tree branches and the rapid death of the entire tree take place which is causing high environm ...
... Recently, there has been an increasing interest among researchers in using combinations of biological control agents to exploit potential synergistic effects among them. In the present study, there were investigated commercially a