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1. "Candidatus Helicobacter heilmannii" from a Cynomolgus Monkey Induces Gastric Mucosa-Associated Lymphoid Tissue Lymphomas in C57BL/6 Mice

Author:
Nakamura, Masahiko; Murayama, Somay Yamagata; Serizawa, Hiroshi; Sekiya, Yukie; Eguchi, Masahiro; Takahashi, Shinichi; Nishikawa, Kaori; Takahashi, Tetsufumi; Matsumoto, Tsukasa; Yamada, Haruki; Hibi, Toshifumi; Tsuchimoto, Kanji; Matsui, Hidenori
Source:
Infection and immunity 2007 v.75 no.3 pp. 1214-1222
ISSN:
0019-9567
Subject:
B-lymphocytes; DNA; Helicobacter heilmannii; Helicobacter pylori; Macaca fascicularis; adenocarcinoma; animal models; bacteria; cytoplasm; genes; lymphoma; mice; mucus; nucleotide sequences; peptic ulcers; ribosomal RNA; sequence analysis; surface area; urease
Abstract:
... Both Helicobacter pylori and "Candidatus Helicobacter heilmannii" infections are associated with peptic ulcers, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphomas. However, good animal models of H. pylori clinical diseases are rare. In this study, we aimed to establish an animal model of "Candidatus Helicobacter heilmannii" gastric MALT lymphoma. We used a ureas ...
DOI:
10.1128/IAI.01459-06
PubMed:
17194807
PubMed Central:
PMC1828597

2. "Electrokinetic Analyte Transport Assay" for α-fetoprotein immunoassay integrates mixing, reaction and separation on-chip

Author:
Kawabata, Tomohisa; Wada, Henry G.; Watanabe, Mitsuo; Satomura, Shinji
Source:
Electrophoresis 2008 v.29 no.7 pp. 1399-1406
ISSN:
0173-0835
Subject:
DNA; antibodies; antigen-antibody complex; blood; electrophoresis; immunoassays; laminar flow; liver neoplasms; mixing
Abstract:
... A rapid and highly sensitive CE immunoassay method integrating mixing, reaction, separation, and detection on-chip is described for the measurement of α-fetoprotein (AFP), a liver cancer marker in blood. Antibody-binding reagents, consisting of 245-bp DNA coupled anti-AFP WA1 antibody (DNA-WA1) and HiLyte dye-labeled anti-AFP WA2 antibody (HiLyte-WA2), and AFP-containing sample were filled into ad ...
DOI:
10.1002/elps.200700898

3. "MALDI-CSI": A proposed method for the tandem detection of human blood and DNA typing from enhanced fingermarks

Author:
Katie Kennedy; Ravell Bengiat; Cameron Heaton; Yael Herman; Carla Oz; Michal Levin Elad; Laura Cole; Simona Francese
Source:
Forensic science international 2021 v.323 pp. 110774
ISSN:
0379-0738
Subject:
DNA; blood; desorption; digestion; forensic sciences; hemoglobin; humans; mass spectrometry; peptides; proteomics
Abstract:
... Matrix Assisted Laser Desorption Ionization Mass Spectrometry Profiling and Imaging (MALDI MSP and MALDI MSI), in combination with bottom up proteomics, have proven to successfully detect and map blood-derived peptide signatures in blood fingermarks, with high specificity and compatibility with a number of blood enhancement techniques (BET). In the present study, the application of MALDI MSP and M ...
DOI:
10.1016/j.forsciint.2021.110774

4. "Reverse degradomics", monitoring of proteolytic trimming by multi-CE and confocal detection of fluorescent substrates and reaction products

Author:
Piccard, Helene; Hu, Jialiang; Fiten, Pierre; Proost, Paul; Martens, Erik; Van den Steen, Philippe E.; Van Damme, Jo; Opdenakker, Ghislain
Source:
Electrophoresis 2009 v.30 no.13 pp. 2366-2377
ISSN:
0173-0835
Subject:
proteolysis; screening; bioactive properties; monitoring; protein-glutamine gamma-glutamyltransferase; cell culture; DNA; fluorescence; data collection; proteinases; chemokine CXCL6; fluorescent labeling; amino acids; mice; neoplasm cells; neutrophils; proteinase inhibitors; electrophoresis
Abstract:
... A platform for profiling of multiple proteolytic activities acting on one specific substrate, based on the use of a 96-channel capillary DNA sequencer with CE-LIF of labeled substrate peptides and reaction products is introduced. The approach consists of synthesis of a substrate peptide of interest, fluorescent labeling of the substrate, either aminoterminally by chemical coupling, or carboxytermi ...
DOI:
10.1002/elps.200800698

5. "pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies."

Author:
Hajib N Madhavan; Moses Y Samson; Murali Ishwarya; Ramanathan Vijayakumar; Malathi Jambulingam
Source:
Virology journal 2010 v.7 no.1 pp. 322
ISSN:
1743-422X
Subject:
DNA; Human betaherpesvirus 5; blood; chi-square distribution; kidney transplant; kidneys; prospective studies; quantitative polymerase chain reaction; virology
Abstract:
... BACKGROUND: The present study was undertaken to determine the rate of occurrence of Human cytomegalovirus (HCMV) among kidney transplant recipients and donors by application of direct detection methods and to understand HCMV infection/disease development among transplanted patients as a prospective study. RESULTS: Peripheral blood samples collected from 76 kidney donors and 76 recipients from Sept ...
DOI:
10.1186/1743-422X-7-322

6. %G+C profiling and cross hybridisation of microbial DNA reveals great variation in below-ground community structure in UK upland grasslands

Author:
Clegg, C.D.; Ritz, K.; Griffiths, B.S.
Source:
Applied soil ecology 2000 v.14 no.2 pp. 125-134
ISSN:
0929-1393
Subject:
chemical composition; upland soils; DNA; guanine; cytosine; soil microorganisms; species diversity; spatial variation; nucleic acid hybridization; grasslands; thermal degradation; grassland soils; United Kingdom

7.   Effects of homobrassinolide in barley callus culture

Author:
A. Temel; N. Gozukirmizi
Source:
Plant, Soil and Environment v.58 no.10 pp. 441-445
ISSN:
1214-1178
Subject:
DNA; DNA methylation; Hordeum vulgare; barley; callus; callus culture; environment; soil; superoxide dismutase; tissue culture
Abstract:
... Thirty-day-old barley (Hordeum vulgare L. cv. Zafer-160) calli were treated with 0, 0.5, 1 µmol/L 22(S),23(S)-Homobrassinolide (HBL) for four weeks. Transfer of calli to hormone-free or HBL-supplemented medium caused an increase in total soluble protein and DNA contents and a decrease in superoxide dismutase (SOD; E.C. 1.15.1.1) activity. 0.5 µmol/L HBL caused the highest protein and D ...
DOI:
10.17221/305/2012-pse

8.  DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa

Author:
P. Prinosilova; R. Rybar; A. Zajicova; J. Hlavicova
Source:
Veterinární medicína v.57 no.3 pp. 133-142
ISSN:
0375-8427
Subject:
DNA; DNA fragmentation; chromatin; cryopreservation; dogs; freeze-thaw cycles; male fertility; semen; sperm motility; spermatozoa; storage time; viability
Abstract:
... Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). In the first experiment, the chromatin integrity of fresh semen from 60 dogs with differing histories of fertility was compared with other sperm parameters (total sperm count, sperm motility, viability ...
DOI:
10.17221/5853-vetmed

9.  Genetic variation in three paddlefish (Polyodon spathula Walbaum) stocks based on microsatellite DNA analysis

Author:
D. Kaczmarczyk; M. Luczynski; P. Brzuzan
Source:
Czech Journal of Animal Science v.57 no.8 pp. 345-352
ISSN:
1212-1819
Subject:
DNA; Polyodon spathula; adults; animal science; fish; founder effect; genetic distance; genetic variation; heterozygosity; loci; microsatellite repeats; Mississippi; Mississippi River; Poland; Ukraine
Abstract:
... There are two stocks of American paddlefish Polyodon spathula in Poland, one in Pogorze and the other in Wasosze. These stocks were established from small quantities of eggs imported from the USA in 1995. In this study, we examined genetic variation at seven microsatellite loci in adult fish from the two Polish farms and one stock farmed in Gorny Tykich in Ukraine. Our data were compared with thos ...
DOI:
10.17221/6269-cjas

10.  Isolation, cloning and sequence analysis of lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs

Author:
A. Erdemir; M. Aktas; N. Dumanli; D. Turgut-Balik
Source:
Veterinární medicína v.57 no.10 pp. 559-567
ISSN:
0375-8427
Subject:
Brown Swiss; DNA; Protozoa; Theileria annulata; Theileria parva; active sites; animal diseases; antiparasitic agents; blood; cows; introns; lactate dehydrogenase; lymphatic diseases; mechanism of action; morbidity; mortality; sequence analysis; theileriosis
Abstract:
... Theileriosis is a serious animal disease that is transmitted by ticks. Theileria species are tick-borne obligate intracellular protozoan parasites that cause severe and mild infections in their hosts. Two of them, Theileria annulata and Theileria parva, cause lymphoproliferative disease with high mortality and morbidity in cattle commonly known as tropical theileriosis and East Coast fever, respec ...
DOI:
10.17221/6368-vetmed

11.  Leaf spot and dieback of Buxus caused by Cylindrocladium buxicola

Author:
I. Šafránková; L. Holková; M. Kmoch
Source:
Plant Protection Science v.49 no.4 pp. 165-168
ISSN:
1212-2580
Subject:
Buxus sempervirens; Calonectria pseudonaviculata; DNA; azoxystrobin; boxwood blight; conidia; dieback; germination; kresoxim-methyl; leaf blight; leaf spot; mancozeb; mycelium; myclobutanil; penconazole; plant protection
Abstract:
... Leaf blight symptoms were observed on potted box plants (Buxus sempervirens cv. Suffruticosa and B. microphylla) in a nursery in South Moravia in August 2010. These symptoms were suggestive of box blight on Buxus spp. Characteristics of the visual symptoms, microscopic features, and identification of the isolates using DNA sequencing are described. The causal agent was isolated and identified as C ...
DOI:
10.17221/82/2012-pps

12.  Metals as a cause of oxidative stress in fish: a review

Author:
M. Sevcikova; H. Modra; A. Slaninova; Z. Svobodova
Source:
Veterinární medicína v.56 no.11 pp. 537-546
ISSN:
0375-8427
Subject:
DNA; antioxidant activity; aquatic environment; biomarkers; enzyme inactivation; ferritin; ferroxidase; fish; gene expression; homeostasis; lipid peroxidation; metabolism; metallothionein; molecular weight; oxidation; oxidative stress; quantitative analysis; reactive oxygen species; redox potential; toxicity
Abstract:
... This review summarizes the current knowledge on the contribution of metals to the development of oxidative stress in fish. Metals are important inducers of oxidative stress in aquatic organisms, promoting formation of reactive oxygen species through two mechanisms. Redox active metals generate reactive oxygen species through redox cycling, while metals without redox potential impair antioxidant de ...
DOI:
10.17221/4272-vetmed

13.  Rapid sexing of selected Galliformes by polymerase chain reaction

Author:
J. Kalina; J. Mucksová; H. Yan; P. Trefil
Source:
Czech Journal of Animal Science v.57 no.4 pp. 187-192
ISSN:
1212-1819
Subject:
DNA; Gallus gallus; Meleagris gallopavo; Phasianus colchicus; W chromosome; chickens; cost effectiveness; grouse; pheasants; phenotype; polymerase chain reaction; sex determination; wood
Abstract:
... Vent sexing of one-day-old chicks in commercial hatcheries has long been common practice and can be highly accurate. However, there are circumstances when this technique is not applicable such as smaller breeds, non-domestic birds, or where is the necessity of precise sexing. In this study we present a simple and reliable method for fast gender determination in selected Galliformes for which pheno ...
DOI:
10.17221/5894-cjas

14.  The effect of high temperature on swine ovarian function in vitro

Author:
AV Sirotkin; M. Kacaniova
Source:
Veterinární medicína v.55 no.8 pp. 377-382
ISSN:
0375-8427
Subject:
DNA; estradiol; genes; heat stress; leptin; progesterone; swine; temperature
Abstract:
... The aim of the present study was to understand the hormonal mechanisms behind the effect of high temperatures on reproductive function. It was proposed that high temperatures can directly alter production of ovarian hormones and/or the response of ovarian cells to hormonal stimulators. To examine this hypothesis, in the 1st series of experiments, we compared the release of progesterone (P4
DOI:
10.17221/2953-vetmed

15.  The structure and role of mammalian sperm RNA: a review

Author:
D. Bukowska; B. Kempisty; H. Piotrowska; P. Sosinska; M. Wozna; S. Ciesiolka; P. Antosik
Source:
Veterinární medicína v.58 no.2 pp. 57-64
ISSN:
0375-8427
Subject:
DNA; chromatin; embryogenesis; genome; haploidy; histones; males; mammals; messenger RNA; microarray technology; oocytes; protamines; spermatogenesis; spermatozoa
Abstract:
... The main role of sperm is the delivery of the paternal genome into the oocyte during fertilisation. However, several lines of evidence have indicated that mammalian spermatozoa contribute more than just their DNA, namely, they also deliver a large range of RNA molecules. Microarray analysis has revealed a complex population of 3000 different kinds of messenger RNA that are delivered to oocytes by ...
DOI:
10.17221/6696-vetmed

16. 'Candidatus Lumbricincola', a novel lineage of uncultured Mollicutes from earthworms of family Lumbricidae

Author:
Nechitaylo, Taras Y.; Timmis, Kenneth N.; Golyshin, Peter N.
Source:
Environmental microbiology 2009 v.11 no.4 pp. 1016-1026
ISSN:
1462-2912
Subject:
Clostridium; DNA; Lumbricidae; Phytoplasma; clones; digestive system; earthworms; fluorescence in situ hybridization; host specificity; nucleotide sequences; ribosomal RNA; sequence analysis; single-stranded conformational polymorphism; Germany; Russia
Abstract:
... Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) have been detected in total rRNA and DNA extracted from tissues, gut contents and casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA sequences exhibited low identity to those of known Mollicutes species and formed a monophyletic cluster distantly affiliated to the 'Candidatus Bacilloplasma' (84. ...
DOI:
10.1111/j.1462-2920.2008.01837.x
PubMed:
19396950

17. 'Gene shaving' as a method for identifying distinct sets of genes with similar expression patterns

Author:
Trevor Hastie; Robert Tibshirani; Michael B Eisen; Ash Alizadeh; Ronald Levy; Louis Staudt; Wing C Chan; David Botstein; Patrick Brown
Source:
Genome biology 2000 v.1 no.2 pp. 57
ISSN:
1474-760X
Subject:
B-lymphocytes; DNA; gene expression; genes; lymphoma; patients
Abstract:
... BACKGROUND: Large gene expression studies, such as those conducted using DNA arrays, often provide millions of different pieces of data. To address the problem of analyzing such data, we describe a statistical method, which we have called 'gene shaving'. The method identifies subsets of genes with coherent expression patterns and large variation across conditions. Gene shaving differs from hierarc ...
DOI:
10.1186/gb-2000-1-2-research0003
PubMed:
11178228
PubMed Central:
PMC15015

18. 'Jumping Jack': Genomic microsatellites underscore the distinctiveness of closely related Pseudoperonospora cubensis and Pseudoperonospora humuli and provide new insights into their evolutionary past

Author:
Nowicki Marcin; Guerry Denita; Trigiano Robert N.; Boggess Sarah L.; Kanetis Loukas; Wadl Phillip A.; Ojiambo Peter S.; Cubeta Marc A.; Spring Otmar; Thines Marco; Runge Fabien; Scheffler Brian E.
Source:
Molecular plant pathology 2021 v.12 no. pp. 686759
ISSN:
1464-6722
Subject:
Cucurbitaceae; DNA; Pseudoperonospora cubensis; Pseudoperonospora humuli; gene flow; genes; genetic variation; genomics; herbaria; microsatellite repeats; mitochondria; plant pathology; population structure
Abstract:
... Downy mildews caused by obligate oomycetes result in severe crop losses worldwide. Among these pathogens, Pseudoperonospora cubensis and P. humuli, two closely related species, adversely affect cucurbits and hops, respectively. Discordant hypotheses concerning their taxonomic relationships have been proposed based on phytopathological evidence and gene sequences of few individuals, but population ...
Handle:
10113/7482132
DOI:
10.3389/fmicb.2021.686759

19. 'Junk' DNA and phenotypic evolution in Silene section Siphonomorpha

Author:
Meagher, Thomas R.; Costich, Denise E.
Source:
Genetics research 2008 v.90 no.1 pp. 111-118
Subject:
Silene; plant genetics; DNA; evolution; nuclear genome; flowers; plant morphology
Abstract:
... One of the long-standing mysteries in genomic evolution is the observation that much of the genome is composed of repetitive DNA, resulting in inter- and intraspecific variation in nuclear DNA content. Our discovery of a negative correlation between nuclear DNA content and flower size in Silene latifolia has been supported by our subsequent investigation of changes in DNA content as a correlated r ...
Handle:
10113/35270
DOI:
10.1017/S001667230700897X
PubMed:
18289405

20. 'LeishMan' topoisomerase I: an ideal chimera for unraveling the role of the small subunit of unusual bi-subunit topoisomerase I from Leishmania donovani

Author:
Ganguly, Agneyo; Das, Benu Brata; Sen, Nilkantha; Roy, Amit; Dasgupta, Somdeb Bose; Majumder, Hemanta K.
Source:
Nucleic acids research 2006 v.34 no.21 pp. 6286-6297
ISSN:
0305-1048
Subject:
DNA; DNA topoisomerase; Leishmania donovani; active sites; catalytic activity; chimerism; humans; tyrosine
Abstract:
... The active site tyrosine residue of all monomeric type IB topoisomerases resides in the C-terminal domain of the enzyme. Leishmania donovani, possesses unusual heterodimeric type IB topoisomerase. The small subunit harbors the catalytic tyrosine within the SKXXY motif. To explore the functional relationship between the two subunits, we have replaced the small subunit of L.donovani topoisomerase I ...
DOI:
10.1093/nar/gkl829
PubMed:
17098934
PubMed Central:
PMC1669778

21. 'Lysobacter enzymogenes ssp. cookii ' Christensen 1978 should be recognized as an independent species, Lysobacter cookii sp. nov

Author:
Kawamura, Yoshiaki; Tomida, Junko; Morita, Yuji; Naka, Takashi; Mizuno, Seiko; Fujiwara, Nagatoshi
Source:
FEMS microbiology letters 2009 v.298 no.1 pp. 118-123
ISSN:
0378-1097
Subject:
DNA; Lysobacter capsici; Lysobacter enzymogenes; fatty acid composition; genome; new species
Abstract:
... 'Lysobacter enzymogenes ssp. cookii' was proposed by Christensen and Cook in 1978; however, this subspecies name has not been cited in the Approved Lists of Bacterial Names and therefore the nomenclature has not been validated. In our genetic approach to clarify the relationships of the designated type strain of 'L. enzymogenes ssp. cookii' PAGU 1119 (GenBank accession number ATCC29488) within the ...
DOI:
10.1111/j.1574-6968.2009.01703.x
PubMed:
19659731

22. The 'kinetochore maintenance loop'--The mark of regulation?

Author:
Brown, William R.A.; Xu, Zheng-yao
Source:
BioEssays 2009 v.31 no.2 pp. 228-236
ISSN:
0265-9247
Subject:
DNA; chromatids; cohesion; epigenetics; humans; interphase; kinetochores; metaphase; mitosis; nucleotide sequences
Abstract:
... Kinetochores can form and be maintained on DNA sequences that are normally non-centromeric. The existence of these so-called neo-centromeres has posed the problem as to the nature of the epigenetic mechanisms that maintain the centromere. Here we highlight results that indicate that the amount of CENP-A at human centromeres is tightly regulated. It is also known that kinetochore assembly requires ...
DOI:
10.1002/bies.200800118

23. (2S)-Naringenin from Typha angustata inhibits vascular smooth muscle cell proliferation via a G₀/G₁ arrest

Author:
Jung-Jin Lee; Hyoseok Yi; In-Su Kim; Yohan Kim; Nguyen Xuan Nhiem; Young Ho Kim; Chang-Seon Myung
Source:
Journal of ethnopharmacology 2012 v.139 no.3 pp. 873-878
ISSN:
0378-8741
Subject:
DNA; Oriental traditional medicine; Typha; antibacterial properties; atherosclerosis; cell cycle; cell proliferation; flow cytometry; immunoblotting; myocardial infarction; myocytes; phosphatidylinositol 3-kinase; phosphorylation; platelet-derived growth factor; protein kinases; smooth muscle; viability
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: Typha angustata is used in traditional Chinese medicine for a variety of clinical disorders. Its pharmacological actions include beneficial effects on hyperlipidemia and myocardial infarction, as well as labor-inducing and antibacterial effects. AIM OF THE STUDY: We investigated the mechanism underlying the ability of (2S)-naringenin, an active compound from Typha a ...
DOI:
10.1016/j.jep.2011.12.038

24. (3-Aminopropyl)-4-methylpiperazine End-capped Poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based Multilayer Films for Gene Delivery

Author:
Li Cuicui; Tzeng Stephany Y.; Tellier Liane E.; Green Jordan J.
Source:
ACS Applied Materials & Interfaces 2013 v.5 no.13 pp. 5947-5953
ISSN:
1944-8252
Subject:
gene transfer; chemical elements; coatings; humans; cytotoxicity; DNA; cations; gene expression; biodegradability
Abstract:
... Biodegradable polyelectrolyte surfaces for gene delivery were created through electrospinning of biodegradable polycations combined with iterative solution-based multilayer coating. Poly(β-amino ester) (PBAE) poly(1,4-butanediol diacrylate-co-4-amino-1-butanol) end-capped with 1-(3-aminopropyl)-4-methylpiperazine was utilized because of its ability to electrostatically interact with anionic molecu ...
DOI:
10.1021/am402115v
PubMed:
23755861
PubMed Central:
PMC3838882

25. (E)-2,4-Bis(p-hydroxyphenyl)-2-butenal enhanced TRAIL-induced apoptosis in ovarian cancer cells through downregulation of NF-κB/STAT3 pathway

Author:
Seung Hee Cho; Mi Hee Park; Hee Peom Lee; Myong Ki Back; Ha Chang Sung; Hee Won Chang; Joo Hwan Kim; Heon-Sang Jeong; Sang Bae Han; Jin Tae Hong
Source:
Archives of pharmacal research 2014 v.37 no.5 pp. 652-661
ISSN:
0253-6269
Subject:
transcription factor NF-kappa B; Maillard reaction; humans; non-specific protein-tyrosine kinase; prognosis; DNA; proteolysis; signal transduction; ovarian neoplasms; antineoplastic agents; neoplasm cells; phosphorylation; animal ovaries; apoptosis; caspase-3
Abstract:
... Ovarian cancer is a cancerous growth arising from the ovary and with poor prognosis that usually have resistant to all currently available treatments. Whether (E)-2,4-bis(p-hydroxyphenyl)-2-butenal (butenal) synthesized by Maillard reaction from fructose–tyrosine, has potential therapeutic activity against human ovarian cancer was investigated using two ovarian cancer cell lines (PA-1, SK-OV-3). W ...
DOI:
10.1007/s12272-013-0326-9

26. (Per)chlorate Reduction by the Thermophilic Bacterium Moorella perchloratireducens sp. nov., Isolated from Underground Gas Storage

Author:
Balk, Melike; van Gelder, Ton; Weelink, Sander A.; Stams, Alfons J.M.
Source:
Applied and environmental microbiology 2008 v.74 no.2 pp. 403-409
ISSN:
0099-2240
Subject:
DNA; Moorella thermoacetica; Moorella thermoautotrophica; cellobiose; chlorates; chlorites; electrons; enzymes; fructose; gases; glucose; mannose; methanol; nitrates; pH; pectins; ribosomal RNA; salinity; sodium chloride; spores; temperature; thermophilic bacteria; xylose
Abstract:
... A thermophilic bacterium, strain An10, was isolated from underground gas storage with methanol as a substrate and perchlorate as an electron acceptor. Cells were gram-positive straight rods, 0.4 to 0.6 μm in diameter and 2 to 8 μm in length, growing as single cells or in pairs. Spores were terminal with a bulged sporangium. The temperature range for growth was 40 to 70°C, with an optimum at 55 to ...
DOI:
10.1128/AEM.01743-07
PubMed:
17981952
PubMed Central:
PMC2223267

27. (Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage

Author:
Balk, Melike; Mehboob, Farrakh; van Gelder, Antonie H.; Rijpstra, W. Irene C.; Damsté, Jaap S. Sinninghe; Stams, Alfons J. M.
Source:
Applied microbiology and biotechnology 2010 v.88 no.2 pp. 595-603
ISSN:
0175-7598
Subject:
DNA; Sporomusa ovata; acetates; acetoin; bacteria; carbon dioxide; enzymes; fructose; methanol; nucleotide sequences; ribosomal RNA; sequence analysis; succinic acid
Abstract:
... A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5-0.8 μm in diameter, and 2-8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various ...
DOI:
10.1007/s00253-010-2788-8
PubMed:
20680263
PubMed Central:
PMC2924991

28. The (TTTA) n polymorphism in intron 4 of CYP19 and the polycystic ovary syndrome risk in a Chinese population

Author:
P. Xu; X. L. Zhang; G. B. Xie; C. W. Zhang; S. M. Shen; X. X. Zhang; Y. X. Cao; W. J. Wang; Y. N. Che; Y. J. Xia; X. K. Wu; L. Yi; Q. Gao; Y. Wang
Source:
Molecular biology reports 2013 v.40 no.8 pp. 5041-5047
ISSN:
0301-4851
Subject:
DNA; alleles; animal ovaries; blood serum; capillary electrophoresis; case-control studies; genotype; introns; microsatellite repeats; patients; polycystic ovary syndrome; polymerase chain reaction; risk; steroid hormones
Abstract:
... Polycystic ovary syndrome (PCOS) is a heterogeneous disease with a strong genetic origin, but the specific determinants are still unknown. The aim of this study was to investigate the association between the (TTTA) ₙ polymorphism in intron 4 of CYP19 and the PCOS risk in a Chinese population. We performed a case–control study which involved 222 PCOS patients and 281 controls. The fluorescent-label ...
DOI:
10.1007/s11033-013-2605-4

29. (−)-Anonaine Induces DNA Damage and Inhibits Growth and Migration of Human Lung Carcinoma H1299 Cells

Author:
Chen, Bing-Hung; Chang, Hsueh-Wei; Huang, Hsuan-Min; Chong, Inn-Wen; Chen, Jia-Shing; Chen, Chung-Yi; Wang, Hui-Min
Source:
Journal of agricultural and food chemistry 2011 v.59 no.6 pp. 2284-2290
ISSN:
0021-8561
Subject:
DNA; DNA damage; anticarcinogenic activity; cell cycle; cell growth; chemoprevention; herbal medicines; humans; lung neoplasms
Abstract:
... The anticancer effects of (−)-anonaine were investigated in this current study. (−)-Anonaine at concentration ranges of 50−200 μM exhibited significant inhibition to cell growth and migration activities on human lung cancer H1299 cells at 24 h, albeit cell cycle analyses showed that (−)-anonaine at the above concentration ranges did not cause any significant changes in cell-cycle distributions. Si ...
DOI:
10.1021/jf103488j

30. (−)-Xanthatin up-regulation of the GADD45γ tumor suppressor gene in MDA-MB-231 breast cancer cells: Role of topoisomerase IIα inhibition and reactive oxygen species

Author:
Shuso Takeda; Momoko Noguchi; Kazumasa Matsuo; Yasuhiro Yamaguchi; Taichi Kudo; Hajime Nishimura; Yoshiko Okamoto; Toshiaki Amamoto; Mitsuru Shindo; Curtis J. Omiecinski; Hironori Aramaki
Source:
Toxicology 2013 v.305 pp. 1-9
ISSN:
0300-483X
Subject:
DNA; DNA damage; acetylcysteine; breast neoplasms; cell death; gene expression regulation; humans; messenger RNA; reactive oxygen species; toxicology; tumor suppressor genes
Abstract:
... Previously, we reported that (−)-xanthatin, a naturally occurring xanthanolide present in the Cocklebur plant, exhibits potent anti-proliferative effects on human breast cancer cells, accompanied by an induction of the growth arrest and DNA damage-inducible gene 45γ (GADD45γ), recognized recently as a novel tumor suppressor gene. However, the mechanisms mediating this activation were unknown. Topo ...
DOI:
10.1016/j.tox.2012.12.019
PubMed:
23313378
PubMed Central:
PMC4030303

31. The -11A of promoter DNA and two conserved amino acids in the melting region of σ⁷⁰ both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interact

Author:
Schroeder, Lisa A.; Choi, Ae-Jin; deHaseth, Pieter L.
Source:
Nucleic acids research 2007 v.35 no.12 pp. 4141-4153
ISSN:
0305-1048
Subject:
DNA; DNA-directed RNA polymerase; RNA; amino acid substitution; amino acids; melting; promoter regions
Abstract:
... Formation of the stable, strand separated, 'open' complex between RNA polymerase and a promoter involves DNA melting of approximately 14 base pairs. The likely nucleation site is the highly conserved -11A base in the non-template strand of the -10 promoter region. Amino acid residues Y430 and W433 on the σ⁷⁰ subunit of the RNA polymerase participate in the strand separation. The roles of -11A and ...
DOI:
10.1093/nar/gkm431
PubMed:
17567604
PubMed Central:
PMC1919498

32. 025 Freezing mammalian semen: If only all sperm were equal!

Author:
William Holt
Source:
Cryobiology 2013 v.67 no.3 pp. 405
ISSN:
0011-2240
Subject:
DNA; Macropodidae; Sarcophilus harrisii; anthropogenic activities; breeding programs; chromatin; cryopreservation; cryoprotectants; cysteine; death; deforestation; disulfide bonds; freezing; gene banks; gene pool; glycerol; inbreeding; interspecific variation; males; mammals; oviductal isthmus; plasma membrane; semen; spermatozoa; thawing; viability; wildlife; wildlife management
Abstract:
... Wildlife extinctions are thought to be occurring more rapidly than at any time in the history of the earth, mainly because of human activities. These include agriculture, urbanization, deforestation and a myriad other processes. While it is impractical to think that reproductive technologies could be used on a grand scale to help save large numbers of species from extinction, the judiciously targe ...
DOI:
10.1016/j.cryobiol.2013.09.031

33. 076 Cryo-preanalytics: From biospecimen research to quality control

Author:
Olga Kofanova
Source:
Cryobiology 2013 v.67 no.3 pp. 419
ISSN:
0011-2240
Subject:
DNA; blood; cold; cold storage; freezing; metabolomics; phosphoproteins; quality control; research projects; temperature
Abstract:
... Biospecimen Research includes biospecimen collection, processing and storage method validation, biospecimen stability studies, biospecimen quality control and research on the impact of preanalytical variables on the quality of the samples. Hypothermic conditions and cryogenic temperatures are part of the critical preanalytical variables. The presentation will show how biospecimen research bridges ...
DOI:
10.1016/j.cryobiol.2013.09.082

34. 081 Ambient temperature stabilization of nucleic acids in human blood

Author:
Rolf Muller
Source:
Cryobiology 2013 v.67 no.3 pp. 420-421
ISSN:
0011-2240
Subject:
DNA; RNA; ambient temperature; biomedical research; blood; cold; diagnostic techniques; freezers; genotyping; humans; hydrolysis; precision medicine; saliva; shipping; transcriptome
Abstract:
... Biospecimen stabilization during collection and transport is of critical importance in the fields of biomedical research and molecular diagnostics. The emergence of personalized medicine has placed an even greater emphasis on the significance of protecting the quality and reliability of clinical samples. The current methods for blood and saliva collection depend heavily on cold-chain logistics and ...
DOI:
10.1016/j.cryobiol.2013.09.087

35. 086 Optimal preservation of DNA Integrity in cat germinal vesicles after microwave-assisted dehydration and supra-zero temperature storage

Author:
Pei-Chih Lee; E. Paramore; M. Van Vorst; L. Weng; D.E. Wildt; G.D. Elliott; P. Comizzoli
Source:
Cryobiology 2013 v.67 no.3 pp. 422
ISSN:
0011-2240
Subject:
DNA; DNA damage; DNA fragmentation; adults; air drying; ambient temperature; animal models; bags; cats; chromatin; equilibrium moisture content; filters; genome; germinal vesicle; glass transition temperature; herring; microwave treatment; staining; storage; storage temperature; titration; trehalose
Abstract:
... The germinal vesicle (GV) is an alternative target to the whole oocyte for exploring new genome preservation approaches at supra-zero temperatures. Using the domestic cat model, we have demonstrated that the GV can survive simple air-drying and then be hydrated to reconstitute a viable oocyte (Graves-Herring et al., 2013). However, this method can lead to variable DNA damage due to uncontrolled wa ...
DOI:
10.1016/j.cryobiol.2013.09.092

36. 094 Application of the Kwei equation for predicting the Tg of stabilizing formulations containing sugars and salts

Author:
Lindong Weng; Ranganathan Vijayaraghavan; Douglas R. MacFarlane; Gloria D. Elliott
Source:
Cryobiology 2013 v.67 no.3 pp. 424
ISSN:
0011-2240
Subject:
DNA; choline; cytochrome c; data collection; differential scanning calorimetry; drugs; equations; foods; glass; glass transition temperature; interleukin-2; lysozyme; models; phosphates; prediction; shelf life; stabilizers; storage temperature; sucrose; thermal stability; trehalose
Abstract:
... Sugars such as trehalose and sucrose have been used in numerous formulations to improve the shelf-life of products ranging from foods to pharmaceuticals and more recently they have also been used as a key protectant for preserving cell-based products. Disaccharides are known to have high glass transition temperatures and thus can immobilize molecules at high storage temperatures. Frequently these ...
DOI:
10.1016/j.cryobiol.2013.09.100

37. 1,2,3,4-Diepoxybutane-Induced DNA–Protein Cross-Linking in Human Fibrosarcoma (HT1080) Cells

Author:
Gherezghiher Teshome B.; Ming Xun; Villalta Peter W.; Campbell Colin; Tretyakova Natalia Y.
Source:
Journal of Proteome Research 2013 v.12 no.5 pp. 2151-2164
ISSN:
1535-3907
Subject:
DNA; DNA damage; DNA repair; air; chemical bonding; cross-linking reagents; crosslinking; cysteine; fibrosarcoma; guanine; histones; humans; mass spectrometry; metabolites; mutagens; platinum; proteolysis; proteome; proteomics; thiols; transcription (genetics); transcription factors; tubulin
Abstract:
... 1,2,3,4-Diepoxybutane (DEB) is the key carcinogenic metabolite of 1,3-butadiene (BD), an important industrial and environmental chemical present in urban air and in cigarette smoke. DEB is a genotoxic bis-electrophile capable of cross-linking cellular biomolecules to form DNA–DNA and DNA–protein cross-links (DPCs). In the present work, mass spectrometry-based proteomics was employed to characteriz ...
DOI:
10.1021/pr3011974
PubMed:
23506368
PubMed Central:
PMC3681427

38. 1,3-Di-n-butylimidazolium tribromide [BBim]Br3: An efficient recyclable catalyst mediated synthesis of N-substituted azepines and their biological evaluation-interaction study with human serum albumin

Author:
Swetha K. Maddili; Rakhi Chowrasia; Vijaya Kumar Kannekanti; Himabindu Gandham
Source:
Journal of photochemistry and photobiology 2018 v.178 pp. 101-107
ISSN:
1011-1344
Subject:
DNA; acetylene; antibacterial properties; aromatic amines; calves; catalysts; drugs; fluconazole; fungi; human serum albumin; ionic liquids; thymus gland; ultraviolet-visible spectroscopy; wavelengths
Abstract:
... A majority of previously reported methods suffer from insufficient yields as well as more complicated experimental procedures, a smaller amount of isolated yields involving time-consuming and tiresome work-up with the use of metal catalyst and restricted scope of substrates. To overcome these issues, an environmentally benign, ionic liquid endorsed multi-component protocol to N-substituted azepine ...
DOI:
10.1016/j.jphotobiol.2017.10.028

39. 1,8-Cineole inhibits root growth and DNA synthesis in the root apical meristem ofBrassica campestris L.

Author:
Ritsuko Koitabashi; Takeshi Suzuki; Tamotsu Kawazu; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa
Source:
Journal of plant research 1997 v.110 no.1 pp. 1-6
ISSN:
0918-9440
Subject:
DNA; allelopathy; antibodies; apical meristems; cell nucleus; cineole; fluorescence microscopy; germination; growth retardation; hypocotyls; mitosis; organelles; root growth; roots; seedlings; thymidine
Abstract:
... 1,8-cineole is a volatile growth inhibitor produced bySalvia species. We examined the effect of this allelopathic compound on the growth of other plants usingBrassica campestris as the test plant. Cineole inhibited germination and growth ofB. campestris in a dosedependent manner. WhenB. campestris was grown for 5 days with various concentrations of cineole, the length of the roots was found to be ...
DOI:
10.1007/BF02506836

40. 1-MCP treatment enhanced expression of genes controlling endosperm cell division and starch biosynthesis for improvement of grain filling in a dense-panicle rice cultivar

Author:
B.B. Panda; A.K. Badoghar; S. Sekhar; B.P. Shaw; P.K. Mohapatra
Source:
Plant science 2016 v.246 pp. 11-25
ISSN:
0168-9452
Subject:
1-methylcyclopropene; DNA; Oryza sativa; biosynthesis; cultivars; cytokinesis; endopolyploidy; endosperm; ethylene; ethylene production; filling period; flowering; fruits; gene expression; genes; inflorescences; rice; signal transduction; starch
Abstract:
... High ethylene production in dense-panicle rice cultivars impacts grain filling. 1-MCP (ethylene action inhibitor) treatment increased assimilates partitioning, cell number and size and expression of starch synthesizing enzyme genes of developing caryopses mostly in the basal spikelets of panicle at early post-anthesis stage. The gain in cell number was less compared to the increase of size. High e ...
DOI:
10.1016/j.plantsci.2016.02.004

41. 1-Methylcyclopropene (1-MCP) treatment differentially mediated expression of vacuolar processing enzyme (VPE) genes and delayed programmed cell death (PCD) during ripening and senescence of apple fruit

Author:
Jingyi Lv; Yingzhi Zhang; Mingyu Sun; Jingxin Chen; Yonghong Ge; Jianrong Li
Source:
Scientia horticulturae 2023 v.307 pp. 111489
ISSN:
0304-4238
Subject:
1-methylcyclopropene; DNA; Malus domestica; apples; climacteric fruits; electrical conductivity; enzymes; ethylene; firmness; malondialdehyde; programmed cell death; reactive oxygen species; transmission electron microscopy; vacuoles
Abstract:
... The main purpose of this research was to evaluate the impacts of 1-methylcyclopropene (1-MCP) treatment on vacuolar processing enzyme (VPE) genes and programmed cell death (PCD) during ripening and senescence of apple fruit (Malus × domestica Borkh.). The ethylene antagonists 1-MCP (1 μL L⁻¹) was applied to apples after harvest. Both treated and control fruit were stored at 20 ± 2°C. The data show ...
DOI:
10.1016/j.scienta.2022.111489

42. 1.688 g/cm3 satellite‐related repeats: a missing link to dosage compensation and speciation

Author:
Miguel Gallach
Source:
Molecular ecology 2015 v.24 no.17 pp. 4340-4347
ISSN:
0962-1083
Subject:
DNA; Drosophila; X chromosome; hybrids; models
Abstract:
... Despite the important progress that has been made on dosage compensation (DC), a critical link in our understanding of the X chromosome recognition mechanisms is still missing. Recent studies in Drosophila indicate that the missing link could be a family of DNA repeats populating the euchromatin of the X chromosome. In this opinion article, I discuss how these findings add a new fresh twist on the ...
DOI:
10.1111/mec.13335

43. The 1.6Å resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer

Author:
Wenbing Tao; Zengqiang Gao; Zhengya Gao; Jiahai Zhou; Zhongxian Huang; Yuhui Dong; Shaoning Yu
Source:
International journal of biological macromolecules 2011 v.48 no.3 pp. 459-465
ISSN:
0141-8130
Subject:
DNA; Escherichia coli; X-ray diffraction; binding sites; crystal structure; cyclic AMP; genes; mutants; nucleotide sequences; proteinases
Abstract:
... The X-ray crystal structure of the cAMP-liganded D138L mutant of Escherichia coli catabolite gene activator protein (CAP) was determined at a resolution of 1.66Å. This high resolution crystal structure reveals four cAMP binding sites in the homodimer. Two anti conformations of cAMPs (anti-cAMP) locate between the β-barrel and the C-helix of each subunit; two syn conformations of cAMPs (syn-cAMP) b ...
DOI:
10.1016/j.ijbiomac.2011.01.009

44. A 1.7-kilobase genomic fragment of alfalfa DNA contains the lectin gene Mslec1

Author:
Brill, L.M.; Konyalian, V.R.; Hirsch, A.M.
Source:
Plant physiology 1995 v.108 no.3 pp. 1311-1312
ISSN:
0032-0889
Subject:
protein synthesis; genetic code; symbiosis; DNA; lectins; Medicago sativa; Ensifer meliloti; genes; nucleotide sequences
PubMed:
7630955
PubMed Central:
PMC157495

45. The 1.8-Å Crystal Structure of the N-Terminal Domain of an Archaeal MCM as a Right-Handed Filament

Author:
Yang Fu; Ian M. Slaymaker; Junfeng Wang; Ganggang Wang; Xiaojiang S. Chen
Source:
Journal of Molecular Biology 2014 v.426 pp. 1512-1523
ISSN:
0022-2836
Subject:
DNA; DNA replication; bacteria; crystal structure; genes; proteins
Abstract:
... Mini-chromosome maintenance (MCM) proteins are the replicative helicase necessary for DNA replication in both eukarya and archaea. Most of archaea only have one MCM gene. Here, we report a 1.8-Å crystal structure of the N-terminal MCM from the archaeon Thermoplasma acidophilum (tapMCM). In the structure, the MCM N-terminus forms a right-handed filament that contains six subunits in each turn, with ...
DOI:
10.1016/j.jmb.2013.12.025

46. 1.92 Angstrom Zinc-Free APOBEC3F Catalytic Domain Crystal Structure

Author:
Nadine M. Shaban; Ke Shi; Ming Li; Hideki Aihara; Reuben S. Harris
Source:
Journal of Molecular Biology 2016 v.428 pp. 2307-2316
ISSN:
0022-2836
Subject:
DNA; Human immunodeficiency virus 1; active sites; catalytic activity; cobalt; crystal structure; crystallization; cytosine; disulfide bonds; divalent metals; pH; pathogens; phylogeny; zinc
Abstract:
... The APOBEC3 family of DNA cytosine deaminases is capable of restricting the replication of HIV-1 and other pathogens. Here, we report a 1.92 Å resolution crystal structure of the Vif-binding and catalytic domain of APOBEC3F (A3F). This structure is distinct from the previously published APOBEC and phylogenetically related deaminase structures, as it is the first without zinc in the active site. We ...
DOI:
10.1016/j.jmb.2016.04.026
PubMed:
27139641
PubMed Central:
PMC5142242

47. IL- 10 gene polymorphism c.-592C>A increases HPV infection susceptibility and influences IL-10 levels in HPV infected women

Author:
Fernanda Costa Brandão Berti; Ana Paula Lombardi Pereira; Kleber Paiva Trugilo; Guilherme Cesar Martelossi Cebinelli; Lorena Flor da Rosa Santos Silva; Marcell Alysson Batisti Lozovoy; Andréa Name Colado Simão; Maria Angelica Ehara Watanabe; Karen Brajão de Oliveira
Source:
Infection, genetics, and evolution 2017 v.53 pp. 128-134
ISSN:
1567-1348
Subject:
DNA; alleles; blood sampling; carcinogenesis; enzyme-linked immunosorbent assay; epithelium; heterozygosity; homozygosity; immunosuppression; interleukin-10; patients; polymerase chain reaction; regression analysis; restriction fragment length polymorphism; women
Abstract:
... Interleukin-10 (IL-10) influences HPV infection and viral persistence, favoring cervical immunosuppression and cervical carcinogenesis. IL-10 levels may be influenced by HPV itself and by IL-10 polymorphisms, including rs1800872 (c.-592C>A). Therefore, we evaluated the influence of IL-10 c.-592C>A polymorphism in HPV infection and in IL-10 plasmatic/cervical levels in HPV infected and non-infected ...
DOI:
10.1016/j.meegid.2017.05.020

48. 10-Plex Digital Polymerase Chain Reaction with Four-Color Melting Curve Analysis for Simultaneous KRAS and BRAF Genotyping

Author:
Tatsuo Nakagawa; Junko Tanaka; Kunio Harada; Akiko Shiratori; Yuzuru Shimazaki; Takahide Yokoi; Chihiro Uematsu; Yoshinobu Kohara
Source:
Analytical chemistry 2020 v.92 no.17 pp. 11705-11713
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; color; dyes; fluorescence; genotyping; liquids; quantitative polymerase chain reaction; silicon; temperature
Abstract:
... Digital PCR (dPCR) is a promising method for performing liquid biopsies that quantifies nucleic acids more sensitively than real-time PCR. However, dPCR shows large fluctuations in the fluorescence intensity of droplets or wells due to insufficient PCR amplification in the small partitions, limiting the multiplexing capability of using the fluorescence intensity. In this study, we propose a measur ...
DOI:
10.1021/acs.analchem.0c01704

49. 100 Years of Chromosome Research in Rye, Secale L.

Author:
Rolf Schlegel
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
DNA; barley; cytogenetic analysis; fish; fluorescence; genes; grasses; haploidy; meiosis; microscopy; mitosis; reciprocal translocation; rice; rye; sequence analysis; tetraploidy; trisomics; wheat
Abstract:
... Although microscopy and genetics were still in their infancy, there are cytological results produced a hundred years ago that are still relevant today. Since the 1920s, rye has been a subject of chromosome research. It started by plotting its mitotic and meiotic chromosomes to determine genome size. After controversial evidence, it became clear that the base number is n = 7. However, structural di ...
DOI:
10.3390/plants11131753

50. The 1001 Arabidopsis DNA Methylomes: An Important Resource for Studying Natural Genetic, Epigenetic, and Phenotypic Variation

Author:
Zhaobo Lang; Shaojun Xie; Jian-Kang Zhu
Source:
Trends in plant science 2016 v.21 no.11 pp. 906-908
ISSN:
1360-1385
Subject:
Arabidopsis; DNA; epigenetics; phenotypic variation
Abstract:
... Intraspecific phenotypic diversity is controlled by natural genetic and epigenetic variation. Kawakatsu et al. recently sequenced the DNA methylomes of a global collection of over 1000 Arabidopsis accessions, and have thereby provided a comprehensive resource for studying natural genetic and epigenetic variation as well as the association of such variation with phenotypic diversity. ...
DOI:
10.1016/j.tplants.2016.09.001
PubMed:
27645898
PubMed Central:
PMC5508569

51. 102 Age-related differences in seminal parameters and expression of fertility marker genes in Marwari stallion

Author:
A. K. Chaudhary; G. N. Purohit; J. S. Mehta; S. K. Ravi; T. R. Talluri
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 158
ISSN:
1031-3613
Subject:
DNA; analysis of variance; artificial vagina; color; computer software; gels; gene expression regulation; mares; pH; plasma membrane; quantitative polymerase chain reaction; sperm concentration; sperm motility; spermatozoa; stallions; viability
Abstract:
... The objective of the present study was to observe age-related differences in seminal parameters and expression of fertility marker genes in Marwari stallions. Semen was collected from 9 Marwari stallions (6 ejaculates per stallion) of 3 different age groups (2 to 4 years, GI; 4 to 6 years, GII; and >6 years, GIII) twice a week using an artificial vagina by allowing the stallions to mount a mare. T ...
DOI:
10.1071/RDv33n2Ab102

52. 108 Fecal metabolite monitoring as a tool to assess sexual maturation in polar bears

Author:
E. Curry; M. A. Stoops; T. L. Roth
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 180
ISSN:
1031-3613
Subject:
DNA; Ursus maritimus; animal ovaries; enzyme immunoassays; excretion; feces; females; humans; juveniles; males; metabolites; monitoring; progeny; progesterone; pseudopregnancy; seasonal variation; sexual maturity; sires; t-test; testosterone
Abstract:
... Polar bears (Ursus maritimus) in managed care generally are paired for breeding starting around the age of 5 or 6 years; however, DNA analyses of the wild population indicate that males as young as 2 or 3 may sire offspring and females as young as 4 can produce cubs. There are no reports describing longitudinal reproductive hormone parameters in juvenile polar bears. The objective of the current s ...
DOI:
10.1071/RDv31n1Ab108

53. IL- 10R1 S138G loss-of-function polymorphism is associated with extrapulmonary tuberculosis risk development in Tunisia

Author:
Walid Ben-Selma; Yosra Ben-Abderrahmen; Jalel Boukadida; Hedi Harizi
Source:
Molecular biology reports 2012 v.39 no.1 pp. 51-56
ISSN:
0301-4851
Subject:
DNA; alleles; genotype; interleukin-10; loss-of-function mutation; molecular biology; odds ratio; polymerase chain reaction; risk; tuberculosis; Tunisia
Abstract:
... There is considerable evidence that host genetic factors are important in determining susceptibility to mycobacterial infections. More recently, functional genetic mutations affecting IL-10 receptor 1 (IL-10R1) were described. In this study, we investigated the relationship of IL-10R1 S138G loss-of-function polymorphism (A536G: rs3135932) with susceptibility to active tuberculosis (TB) in Tunisian ...
DOI:
10.1007/s11033-011-0709-2

54. 11-Deoxycortisol is a corticosteroid hormone in the lamprey

Author:
Close, David A.; Yun, Sang-Seon; McCormick, Stephen D.; Wildbill, Andrew J.; Li, Weiming
Source:
Proceedings of the National Academy of Sciences of the United States of America 2010 v.107 no.31 pp. 13942-13947
ISSN:
0027-8424
Subject:
DNA; Petromyzon marinus; adrenal cortex hormones; binding properties; cytosol; high performance liquid chromatography; mass spectrometry; metabolism; radioimmunoassays; sex hormones; signal transduction; sodium-potassium-exchanging ATPase; stress response; vertebrates
Abstract:
... Corticosteroid hormones are critical for controlling metabolism, hydromineral balance, and the stress response in vertebrates. Although corticosteroid hormones have been well characterized in most vertebrate groups, the identity of the earliest vertebrate corticosteroid hormone has remained elusive. Here we provide evidence that 11-deoxycortisol is the corticosteroid hormone in the lamprey, a memb ...
DOI:
10.1073/pnas.0914026107
PubMed:
20643930
PubMed Central:
PMC2922276

55. 114 Effect of in vivo heat stress on DNA methylation and DNA hydroxymethylation of bovine oocytes

Author:
F. A. Diaz; E. J. Gutierrez; B. A. Foster; P. T. Hardin; K. R. Bondioli
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 183
ISSN:
1031-3613
Subject:
DNA; DNA methylation; antibodies; asses; beef cows; cold; epigenetics; fluorescence; fluorescent antibody technique; fluorescent dyes; germinal vesicle; goats; heat stress; immunoglobulin G; immunohistochemistry; least squares; metaphase; ovarian follicles; spring; summer; transcription (genetics); Louisiana
Abstract:
... Cattle under the effect of heat stress have reduced fertility, with negative effects on the oocyte observed at the morphological, biochemical, transcriptional and developmental levels. There are no studies evaluating the effect of heat stress on the epigenetic profile of bovine oocytes, which plays a fundamental role in the regulation of gamete development. The objective of this study was to evalu ...
DOI:
10.1071/RDv31n1Ab114

56. 114 Omega-3-enriched diet improves fertilization competence of cryopreserved sperm

Author:
D. Kalo; D. Reches; A. Komsky-Elbaz; U. Moallem; Y. Zeron; Z. Roth
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 184
ISSN:
1031-3613
Subject:
DNA; DNA fragmentation; Holstein; acrosome; blastocyst; bulls; cell viability; cryopreservation; dairy herds; diet; fatty acid composition; flow cytometry; freeze-thaw cycles; linseed oil; male fertility; membrane potential; mitochondrial membrane; oocytes; reactive oxygen species; saturated fatty acids; semen quality; slaughterhouses; sperm quality; straw
Abstract:
... Intensive reproductive management in dairy herds is mostly based on AI using high-merit bulls. Therefore, semen quality of bulls is of high importance. An association between semen quality and fatty acid content in feed has been suggested. Accordingly, the aim of this study was to examine the effect of omega-3 supplementation on sperm traits and fertilization competence. Fifteen Israeli Holstein b ...
DOI:
10.1071/RDv32n2Ab114

57. 119 Short spermatozoa-oocyte co-incubation improves outcomes of IVF in sheep

Author:
D. Anzalone; M. Czernik; L. Palazzese; Y. Ressaissi; P. Scapolo; P. Loi
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 186
ISSN:
1031-3613
Subject:
DNA; Pisum sativum; agglutinins; blastocyst; blood serum; differential staining; embryogenesis; estrus; humans; in vitro fertilization; isoproterenols; oocytes; polyspermy; propidium; reproductive performance; risk; sheep; spermatozoa; zona pellucida
Abstract:
... The assisted reproductive technique IVF is routinely applied in humans and large animals, both to boost reproductive performance and also for basic research. Despite its value, IVF has seen very little progress in the last two decades and relies on established paradigms, such as overnight sperm-egg co-incubation. However, the long exposure of oocytes to spermatozoa in a dish increases the risk of ...
DOI:
10.1071/RDv32n2Ab119

58. A 12-deoxywithastramonolide-rich somaclonal variant in Withania somnifera (L.) Dunal—molecular cytogenetic analysis and significance as a chemotypic resource

Author:
Satiander Rana; Niha Dhar; Wajid W. Bhat; Sumeer Razdan; Shabnam Khan; Rekha S. Dhar; Prabhu Dutt; Surrinder K. Lattoo
Source:
In vitro cellular & developmental biology 2012 v.48 no.5 pp. 546-554
ISSN:
1054-5476
Subject:
DNA; Panax; Withania somnifera; chromosome number; chromosomes; clones; cytogenetic analysis; explants; genetic polymorphism; high performance liquid chromatography; indole butyric acid; kinetin; leaves; medicinal plants; meiosis; mitosis; organogenesis; phenotype; random amplified polymorphic DNA technique; secondary metabolites; somaclonal variation
Abstract:
... Withania somnifera, commonly known as ashwagandha or Indian ginseng, is a valuable medicinal plant, synthesizing a wide array of pharmacologically active secondary metabolites known as withanolides. In this study, we investigated variation among 54 regenerated plants attained through indirect organogenesis from leaf explants. Organogenic calli were induced on Murashige and Skoog medium containing ...
DOI:
10.1007/s11627-012-9458-8

59. 12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

Author:
Lina Tortorici; Rosalia Di Gerlando; Marco Tolone; Salvatore Mastrangelo; Maria Teresa Sardina
Source:
Livestock science 2016 v.193 pp. 39-44
ISSN:
1871-1413
Subject:
Capra hircus; DNA; adulterated products; agarose; blood; breeds; cheeses; cows; electrophoresis; ewe milk; ewes; gels; genes; goat milk; goats; milk; mitochondria; ribosomal RNA; traceability
Abstract:
... For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairy products, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheeses of Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovis aries) mitochondrial species-specific gene fragment. The use of spec ...
DOI:
10.1016/j.livsci.2016.09.015

60. IL- 13 downregulates PPAR-γ/heme oxygenase-1 via ER stress-stimulated calpain activation: aggravation of activated microglia death

Author:
Liu, Shing Hwa; Yang, Cheng Ning; Pan, Hung Chuan; Sung, Yen Jen; Liao, Ko Kaung; Chen, Wen Bao; Lin, Wen Zheng; Sheu, Meei Ling
Source:
Cellular and molecular life sciences 2010 v.67 no.9 pp. 1465-1476
ISSN:
1420-682X
Subject:
DNA; agonists; antagonists; apoptosis; calpain; caspase-12; death; endoplasmic reticulum; heme oxygenase (biliverdin-producing); interleukin-10; interleukin-13; lipopolysaccharides; neuroglia; protein synthesis; small interfering RNA; transcriptional activation; transfection
Abstract:
... Interleukin 13 (IL-13) has been shown to induce the death of activated microglia. We observed that IL-13, but not IL-4 or IL-10, significantly enhanced endoplasmic reticulum (ER) stress induction, apoptosis and death in microglia activated by lipopolysaccharide (LPS). IL-13 enhanced ER stress-regulated calpain activation and calpain-II expression in LPS-activated microglia. Calpain-II siRNA effect ...
DOI:
10.1007/s00018-009-0255-4

61. 13-Acetoxysarcocrassolide Exhibits Cytotoxic Activity against Oral Cancer Cells through the Interruption of the Keap1/Nrf2/p62/SQSTM1 Pathway: The Need to Move Beyond Classical Concepts

Author:
Yi-Chang Liu; Bo-Rong Peng; Kai-Cheng Hsu; Mohamed El-Shazly; Shou-Ping Shih; Tony Eight Lin; Fu-Wen Kuo; Yi-Cheng Chou; Hung-Yu Lin; Mei-Chin Lu
Source:
Marine drugs 2020 v.18 no.8 pp. -
ISSN:
1660-3397
Subject:
DNA; Western blotting; acetylcysteine; animal models; antineoplastic activity; antineoplastic agents; antioxidant enzymes; apoptosis; cell viability; corals; cytotoxicity; enzyme activity; flow cytometry; fluorescent antibody technique; immunostimulants; mechanism of action; membrane potential; mitochondrial membrane; mouth neoplasms; neoplasm cells; precipitin tests; proteins; reactive oxygen species; small interfering RNA; toxicity testing; transfection; xenotransplantation
Abstract:
... 13-Acetoxysarcocrassolide (13-AC), a marine cytotoxic product isolated from the alcyonacean coral Lobophytum crassum, exhibited potent antitumor and immunostimulant effects as reported in previous studies. However, the 13-AC antitumor mechanism of action against oral cancer cells remains unclear. The activity of 13-AC against Ca9-22 cancer cells was determined using MTT assay, flow cytometric anal ...
DOI:
10.3390/md18080382

62. 135 Strategies for transfection of bovine mesenchymal stem cells with pBC1-anti-CD3 vector

Author:
F. B. Duarte; S. N. Báo; M. Brígido; J. M. Araújo; E. d. O. Melo; C. F. Martins
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 194
ISSN:
1031-3613
Subject:
DNA; Southern blotting; T-lymphocytes; adipocytes; adipose tissue; air; autoimmune diseases; bioreactors; carbon dioxide; cattle; culture dishes; fetal bovine serum; fibroblasts; flow cytometry; fluorescence; fluorescence microscopy; funding; genetically modified organisms; human diseases; humidity; mesenchymal stromal cells; monoclonal antibodies; necrosis; neomycin; polymerase chain reaction; transfection
Abstract:
... Cells from different origins behave differently regarding the incorporation of exogenous genetic material and the formation of transgenic cells. In this context, the objective of this study was to verify the potential of transfection of bovine mesenchymal stem cells from Wharton's jelly and adipose tissue, comparing two transfection protocols, using Lipofectamine LTX and Plus or Xfect reagents, wi ...
DOI:
10.1071/RDv32n2Ab135

63. 138 Associations of sperm head morphometrics with quality parameters of frozen-thawed ram semen

Author:
J. Navaranjan; J. Szymanowicz; M. Murawski; T. Schwarz; P. M. Bartlewski
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 195
ISSN:
1031-3613
Subject:
DNA; Merino; acrosome; alkaline phosphatase; artificial vagina; aspartate transaminase; biomarkers; breeding season; chromatin; computer software; conception; droplets; eosin; ewes; freeze-thaw cycles; membrane permeability; mitochondria; morphometry; multivariate analysis; nonthermal processing; ova; pregnancy rate; rams; semen extenders; seminal plasma; sperm motility; sperm quality; staining; straw; viability
Abstract:
... Current methods of mammalian semen evaluation focus on determining spermatozoa motility, concentration, mitochondrial status, and nucleus or chromatin structure integrity, quantifying their ability to bind to ova or measuring seminal plasma content of various biochemical markers. However, there is a paucity of studies that address relationships between sperm head morphometry (the external shape an ...
DOI:
10.1071/RDv32n2Ab138

64. 139 sex-specific gene expression in porcine pre-implantation embryos

Author:
D. Kradolfer; J. Knubben; V. Flöter; J. Bick; S. Bauersachs; S. E. Ulbrich
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 199
ISSN:
1031-3613
Subject:
DNA; German Landrace; National Center for Biotechnology Information; Pietrain; X chromosome; autosomes; blastocyst; boars; embryogenesis; gene expression; genes; genome assembly; in vitro fertilization; messenger RNA; microRNA; nitrogen; ovulation; pH; sows; spermatozoa; statistical analysis; transcription (genetics); uterus; California
Abstract:
... X-Chromosome inactivation in female mammals starts during early blastocyst stage with expression of the X-inactive specific transcript (XIST), which coats and silences the inactive X chromosome. However, this compensation is not complete in blastocysts, as a large number of X-linked transcripts are more highly expressed in female embryos than in males. Furthermore, the process of X chromosome inac ...
DOI:
10.1071/RDv28n2Ab138

65. 14 Composition of Semen Extenders and Varying Seminal Plasma Concentrations Affects Formation of Neutrophil Extracellular Traps in the Bovine System

Author:
T. Fichtner; F. Kotarski; L. Silva; C. Hermosilla; A. Taubert; C. Wrenzycki
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 146-147
ISSN:
1031-3613
Subject:
DNA; additives; analysis of variance; animal proteins; artificial insemination; bacteria; bulls; business enterprises; centrifugation; coculture; egg yolk; eggs; females; ficoll; immunomodulators; insemination; micrococcal nuclease; neutrophils; pathogens; phospholipids; protein sources; secretion; semen extenders; seminal plasma; spermatozoa; uterus; vagina
Abstract:
... Neutrophils are recruited into the female reproductive tract following insemination to eliminate excess spermatozoa and bacteria introduced by the breeding process. In natural breeding, bovine semen is deposited in the vagina and sperm migrate into the uterus, leaving the bulk of seminal plasma (SP) behind. Current artificial insemination protocols introduce variable amounts of SP and semen extend ...
DOI:
10.1071/RDv30n1Ab13

66. 14 Telomere length in cloned camels produced by somatic cell nuclear transfer is not different from that in their naturally produced counterparts

Author:
N. A. Wani; K. P. Kumar
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 114
ISSN:
1031-3613
Subject:
Camelus dromedarius; DNA; algorithms; blood; camels; chemiluminescence; chloroform; computer software; diagnostic techniques; digoxigenin; gels; hybridization; hybridization probes; molecular weight; neutralization; nucleic acid hybridization; nylon; phenol; progeny; somatic cells; t-test; telomeres; temperature; totipotency; Germany
Abstract:
... There are controversial reports on the restoration of eroded telomere length in offspring produced by somatic cell nuclear transfer (SCNT) in different animal species. To the best of our knowledge, no earlier studies report telomere length in naturally produced or cloned animals in any of the camelid species. Therefore, the present study was conducted to estimate the telomere length in dromedary c ...
DOI:
10.1071/RDv33n2Ab14

67. 14 epigenetic changes in equine spermatozoa during cryopreservation: a preliminary study

Author:
C. Aurich; B. Schreiner; N. Ille; M. Alvarenga; D. Scarlet
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 136-137
ISSN:
1031-3613
Subject:
DNA; DNA methylation; Shetland Pony; breeding; computer software; conception; cooling; cryopreservation; cryoprotectants; cytosine; denaturation; enzyme-linked immunosorbent assay; epigenetics; freeze-thaw cycles; freezing; glycerol; insemination; mares; monoclonal antibodies; semen; spermatozoa; stallions; statistical analysis; California; Sweden
Abstract:
... The use of cryopreserved semen for insemination of mares facilitates breeding management but often results in reduced conception rates. This has been mainly attributed to changes in sperm membrane function caused by the freezing-thawing procedure. However, semen processing may also contribute to epigenetic changes in spermatozoa. In the present study, we therefore addressed changes in sperm DNA-me ...
DOI:
10.1071/RDv28n2Ab13

68. 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers

Author:
Rajagopalan, Sridharan; Jaulent, Agnes M.; Wells, Mark; Veprintsev, Dmitry B.; Fersht, Alan R.
Source:
Nucleic acids research 2008 v.36 no.18 pp. 5983-5991
ISSN:
0305-1048
Subject:
DNA; DNA damage; acetylation; dissociation; neoplasms; phosphorylation; post-translational modification; proteins; serine; ultracentrifugation
Abstract:
... Activation of the tumour suppressor p53 on DNA damage involves post-translational modification by phosphorylation and acetylation. Phosphorylation of certain residues is critical for p53 stabilization and plays an important role in DNA-binding activity. The 14-3-3 family of proteins activates the DNA-binding affinity of p53 upon stress by binding to a site in its intrinsically disordered C-termina ...
DOI:
10.1093/nar/gkn598
PubMed:
18812399
PubMed Central:
PMC2566891

69. 14-Deoxy-11,12-didehydroandrographolide inhibits proliferation and induces GSH-dependent cell death of human promonocytic leukemic cells

Author:
Rahul Raghavan; Sanith Cheriyamundath; Joseph Madassery
Source:
Natural medicines 2014 v.68 no.2 pp. 387-394
ISSN:
1340-3443
Subject:
Andrographis paniculata; DNA; Western blotting; apoptosis; caspase-3; cell viability; cytotoxicity; glutathione; humans; inhibitory concentration 50; leukemia
Abstract:
... 14-Deoxy-11,12-didehydroandrographolide (AND2), an analogue of andrographolide, showed more potent cytotoxicity against human promonocytic leukemia (THP-1) cells than adherent cancer cell lines. In this study AND2 was isolated from the plant Andrographis paniculata and it was characterized. The antiproliferative effect of AND2 on both adherent (PC-3 and MDAMB) and non-adherent (THP-1 and Jurkat) c ...
DOI:
10.1007/s11418-014-0815-2
PubMed:
24458985

70. 141 Effect of combined treatment of melatonin and equine chorionic gonadotrophin on fresh semen quality of Beetal bucks during the non-breeding season

Author:
M. Abbas; M. Irfan-ur-Rehman Khan; A. Rehman; N. Hameed; I. Mohsin; M. Younis; M. Bilal; M. Shahzad
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 197
ISSN:
1031-3613
Subject:
Beetal; DNA; analysis of variance; artificial vagina; breeding; breeding season; bucks; color; conception; ears; enzyme-linked immunosorbent assay; equine chorionic gonadotropin; kinematics; linear models; melatonin; mitochondria; pH; radioimmunoassays; semen quality; sperm concentration; spermatozoa; subtropics; testosterone; variance; viability; Pakistan
Abstract:
... In the subtropics, bucks show seasonal breeding patterns, and their semen quality decreases during the non-breeding season. Therefore, breeders tend to improve bucks’ semen quality before the breeding season for higher conception rates. In the current study, we hypothesised that simultaneous administration of equine chorionic gonadotrophin (ECG) and melatonin would improve fresh semen quality in b ...
DOI:
10.1071/RDv32n2Ab141

71. 142 Nulliparity alters gene expression in inner cell mass and trophoblast of equine blastocysts in old mares

Author:
E. Derisoud; L. Jouneau; C. Archilla; N. Daniel; Y. Jaszczyszyn; C. Gourtay; A. Margat; M. Dahirel; N. Peynot; L. Briot; F. De Geoffroy; V. Duranthon; P. Chavatte-Palmer
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 179
ISSN:
1031-3613
Subject:
DNA; RNA; embryonic mortality; extracellular matrix; foals; focal adhesions; gene expression; gene expression regulation; gene ontology; genes; immune response; mares; models; myosin; nucleosomes; plasma membrane; proteasome endopeptidase complex; protein metabolism; ribosomes; semen; sphingolipids; stallions; trophoblast
Abstract:
... An increased incidence in early embryo loss has been observed in aged mares. Moreover, the first foal born to a mare is lighter than her subsequent foals, with reported impaired placental function at term. Because trophoblast function may be affected from the embryo stage, the aim of this project was to determine the effect of parity in aged mares on gene expression in Day-8.5 embryos. Middle-aged ...
DOI:
10.1071/RDv33n2Ab142

72. 144 Lack of correspondence between commercial genetic test variants for type 2 polysaccharide storage myopathy and a histopathologic diagnosis in Quarter Horses

Author:
K. Herrick; M. Henry; J. Petersen; D. Velez-Irizarry; C. Finno; S. Valberg
Source:
Journal of equine veterinary science 2021 v.100 pp. 103607
ISSN:
0737-0806
Subject:
DNA; Quarter Horse; alleles; biopsy; blood; filamin; genotyping; glycogen (starch) synthase; heterozygosity; high-throughput nucleotide sequencing; histology; histopathology; homozygosity; horses; loci; muscle tissues; muscles; muscular diseases; mutation; polysaccharides; veterinary medicine
Abstract:
... A diagnosis of type 2 polysaccharide storage myopathy (PSSM2) in Quarter Horse-related breeds (QH) is currently based on the absence of the glycogen synthase 1 (GYS1) mutation causing type 1 PSSM (PSSM1) and presence of aggregates polysaccharide in myofibers. Recently, genetic tests for PSSM2 have been offered by a commercial company based on variants in the genes myotilin (termed P2), filamin C ( ...
DOI:
10.1016/j.jevs.2021.103607

73. 144 Sex ratio of in vitro-produced goat embryos

Author:
M. Stoltzfus; J. Wayman; R. Stilz; D. Bresnahan
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 197
ISSN:
1031-3613
Subject:
DNA; agarose; blastocyst; culture media; embryogenesis; ethidium; females; gels; genes; glucose; goat meat; goats; in vitro culture; in vitro fertilization; males; metabolism; milk; oocytes; polymerase chain reaction; sex ratio; slaughterhouses; small farms; spermatozoa; uterus
Abstract:
... Goats are important livestock species because they produce meat, milk, and fibre and are also easily maintainable on small farms. Although goats provide many products and consumption of goat meat is increasing in the United States, the industry lags compared with many species with regard to IVF techniques to enhance goat production. It has been demonstrated in other species that male IVF embryos t ...
DOI:
10.1071/RDv31n1Ab144

74. 145 Effect of Dietary Organic Zinc Levels on Boar (Sus scrofa domesticus) Sperm DNA Fragmentation Dynamics

Author:
A. Martínez; J. Gosálvez; C. López-Fernández; J. A. G. González; C. G. Artiga; Y. D. L. Ortega; M. E. Kjelland; A. García-Contreras
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 212
ISSN:
1031-3613
Subject:
DNA; DNA fragmentation; boars; chromatin; cytotoxicity; diet; fluorescence microscopy; semen extenders; spermatozoa; staining; thawing; zinc
Abstract:
... Zinc (Zn) is essential for the development and activity of sperm, although its cytotoxic effect on sperm has been little studied. This study evaluated the effect of organic Zn; that is, Zn-methionate (Zn-Met), on the DNA fragmentation of boar sperm. Domestic boars (York×Landrace, Sus scrofa domesticus; n=15) were randomly allocated into 3 levels of Zn dietary concentrations: 25 (Control), 150, or ...
DOI:
10.1071/RDv30n1Ab144

75. 147 Serological and molecular detection of Anaplasma in East Texas horses

Author:
N. Shost; A. Russell; L. Lopez; J. Bechelli; J.K. Suagee-Bedore
Source:
Journal of equine veterinary science 2021 v.100 pp. 103610
ISSN:
0737-0806
Subject:
Anaplasma phagocytophilum; DNA; Ixodes scapularis; anaplasmosis; antibodies; blood serum; death; edema; fever; fluorescent antibody technique; genes; horses; muscles; polymerase chain reaction; seroprevalence; surveys; ticks; veterinary medicine; New Jersey; New York; Texas
Abstract:
... Anaplasma phagocytophilum (AP) is a tick-borne bacteria that cycles between the tick, Ixodes scapularis and the hematopoietic cells of vertebrate hosts. AP is responsible for the disease equine granulocytic anaplasmosis, a generally transient infection characterized by fever, depression, inappetence, ventral edema petechiae, icterus, ataxia, recumbency, muscle stiffness, and, in severe cases, deat ...
DOI:
10.1016/j.jevs.2021.103610

76. 149 DNA methylation and regulation of DNMTs in freeze tolerant wood frog, Rana sylvatica

Author:
Jing Zhang; Kenneth B. Storey
Source:
Cryobiology 2013 v.67 no.3 pp. 440
ISSN:
0011-2240
Subject:
Lithobates sylvaticus; freezing; methyltransferases; energy; DNA methylation; liver; protein content; glucose; muscles; DNA; protein synthesis; cytosine; habitats; frogs; transcription (genetics); genomics; temperature; enzyme activity; cold tolerance; tissues
Abstract:
... Wood frog (Rana sylvatica) is one of the few vertebrate species that have perfected the biological adaptations of surviving whole-body freezing when the temperature falls below 0°C in its natural habitat. It has been established that the metabolic rate is coordinated at different stages of the whole-body freezing of wood frog. Energy expensive and optional biological processes need to be repressed ...
DOI:
10.1016/j.cryobiol.2013.09.155

77. 149 Uptake of C18:0 from culture media during invitro culture decreases cryosurvival rates of bovine embryos

Author:
I. Bertijn; B. M. Gadella; H. T. A. van Tol; A. Rijneveld; P. L. A. M. Vos; H. Aardema
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 183
ISSN:
1031-3613
Subject:
DNA; apoptosis; blastocyst; bovine serum albumin; cattle; cryopreservation; embryo culture; embryo transfer; embryogenesis; free fatty acids; freeze-thaw cycles; lipid composition; necrosis; nucleotidyltransferases; oleic acid; oviducts; ovulation; regression analysis; slaughterhouses; stearic acid; stoichiometry
Abstract:
... Cryosurvival of invitro-produced bovine embryos is lower than that of invivo-produced embryos, limiting their usability in the field. Previous work showed that the embryo’s lipid composition relates to its quality and cryosurvival. The present study aimed to investigate the effects of free fatty acid (FA) additions to embryo culture media during the oviduct phase of embryonic development on the im ...
DOI:
10.1071/RDv33n2Ab149

78. IL- 15 aggravates atherosclerotic lesion development in LDL receptor deficient mice

Author:
Thomas van Es; Gijs H.M. van Puijvelde; Ingrid N. Michon; Eva J.A. van Wanrooij; Paula de Vos; Niels Peterse; Theo J.C. van Berkel; Johan Kuiper
Source:
Vaccine 2011 v.29 no.5 pp. 976-983
ISSN:
0264-410X
Subject:
DNA; atherosclerosis; cytotoxicity; diet; hypercholesterolemia; interleukin-15; leukocytes; low density lipoprotein; macrophages; mice; neutralization; spleen; splenocytes; tumor necrosis factor-alpha; vaccination; vaccines
Abstract:
... BACKGROUND: Interleukin 15 (IL-15) is a pro-inflammatory cytokine involved in inflammatory diseases and IL-15 is expressed in atherosclerotic plaques. METHODS: To establish the role of IL-15 in atherosclerosis we studied the effect of IL-15 on atherosclerosis associated cells in vitro and in vivo by neutralizing IL-15 using a DNA vaccination strategy. RESULTS: Upon feeding a Western type diet LDLr ...
DOI:
10.1016/j.vaccine.2010.11.037

79. 151 Modulating Nrf2 transcription factor activity by posttranslational modifications and protein–protein interactions: Revealing the regulatory mechanisms of antioxidant defenses during hibernation

Author:
Shannon N. Tessier; Kenneth B. Storey
Source:
Cryobiology 2013 v.67 no.3 pp. 441
ISSN:
0011-2240
Subject:
DNA; Sciuridae; acetylation; antioxidant activity; antioxidant genes; enzymes; gene expression regulation; heat production; hibernation; homeostasis; mammals; oxygen consumption; phosphorylation; precipitin tests; protein-protein interactions; reactive oxygen species; skeletal muscle; transcription factors; ubiquitination; viability
Abstract:
... A common feature of organismal response to environmental stress is metabolic rate depression coupled with enhancement of cytopreservation strategies to preserve viability until the stress passes. Mammalian hibernation is an example of this survival strategy. One of the important cytoprotective features needed for hibernation are antioxidant defenses which counteract reactive oxygen species (ROS) g ...
DOI:
10.1016/j.cryobiol.2013.09.157

80. 154 Adaptations of cardiac muscle function during mammalian hibernation

Author:
Shannon N. Tessier; Bryan Luu, Kenneth B. Storey
Source:
Cryobiology 2013 v.67 no.3 pp. 441-442
ISSN:
0011-2240
Subject:
DNA; GATA transcription factors; Spermophilus; blood; body temperature; cold; genes; heart rate; hibernation; mammals; muscles; myocardium; protein content; troponin C; troponin I; viscosity
Abstract:
... Entry into torpor during hibernation has major consequences for mammalian hearts. The heart must continue to beat at cold body temperatures and although heart rate is much slower, contractile force must increase to deal with the higher viscosity of blood. Muscle remodelling is needed. We examined responses of the GATA-4 and Nkx2-5 transcription factors that are essential regulators of cardiac hype ...
DOI:
10.1016/j.cryobiol.2013.09.160

81. The 156KELK159 tetrapeptide of HIV-1 integrase is critical for lentiviral gene integration

Author:
Wen-juan Li; Lu Huang; Jian-qi Zhang; Guan-lan Xu; Ling Tian; Jing-lun Xue; Jin-zhong Chen; William Jia
Source:
Molecular biology reports 2012 v.39 no.1 pp. 343-349
ISSN:
0301-4851
Subject:
DNA; DNA replication; gene expression; integrases; molecular biology; mutants; physiological transport; quantitative polymerase chain reaction; reporter genes; reverse transcriptase polymerase chain reaction; two hybrid system techniques; virion; viruses
Abstract:
... HIV-1 integrase (HIV-1 IN), a key element of HIV-1-derived lentiviral vectors, is crucial for the stable maintenance of the vector gene by inserting them into host genome. HIV-1 IN has been found to have functions other than integration, such as involving in virion morphology, viral DNA synthesis and viral DNA nuclear import. In our study, the yeast two-hybrid assay identified a tetrapeptide 156KE ...
DOI:
10.1007/s11033-011-0744-z

82. 15N2-DNA-stable isotope probing of diazotrophic methanotrophs in soil

Author:
Buckley, D.H.; Huangyutitham, V.; Hsu, S.F.; Nelson, T.A.
Source:
Soil biology & biochemistry 2008 v.40 no.6 pp. 1272-1283
ISSN:
0038-0717
Subject:
Methylocystis; food webs; nitrogen fixation; soil bacteria; nitrogen; genome; DNA; evolution; cellobiose; methane; microbial ecology; carbon; 4-hydroxybenzoic acid; provenance; methanotrophs; stable isotopes; New York
Abstract:
... Nucleic acid stable isotope probing (SIP) is a powerful tool that can identify and characterize the microorganisms that mediate specific soil processes and explore the flow of C and N through functional groups in the soil food web. While 13C-SIP has been used successfully in a range of applications, methodological constraints have limited the applicability of 15N-labelled compounds in nucleic acid ...
DOI:
10.1016/j.soilbio.2007.05.006

83. 16S Amplicon Sequencing of Nitrifying Bacteria and Archaea Inhabiting Maize Rhizosphere and the Influencing Environmental Factors

Author:
Oluwatobi Esther Ayiti; Ayansina Segun Ayangbenro; Olubukola Oluranti Babalola
Source:
Agriculture (Basel) 2022 v.12 no.9 pp. -
ISSN:
2077-0472
Subject:
Archaea; DNA; Nitrobacter; Nitrococcus; Nitrosococcus; Nitrosomonas; Nitrosospira; Nitrosovibrio; Nitrospirae; agriculture; ammonia; corn; nitrates; nitrogen; rhizosphere; soil; species diversity
Abstract:
... Nitrifying bacteria and archaea are ubiquitous and can transform ammonia locked up in soil or manure into nitrate, a more soluble form of nitrogen. However, nitrifying bacteria and archaea inhabiting maize rhizosphere have not been fully explored. This study evaluates the diversity and abundance of nitrifying bacteria and archaea across different growth stages of maize using 16S amplicon sequencin ...
DOI:
10.3390/agriculture12091328

84. 16S rRNA Gene Pyrosequencing Reveals Shift in Patient Faecal Microbiota During High-Dose Chemotherapy as Conditioning Regimen for Bone Marrow Transplantation

Author:
Emmanuel Montassier; Eric Batard; Sébastien Massart; Thomas Gastinne; Thomas Carton; Jocelyne Caillon; Sophie Le Fresne; Nathalie Caroff; Jean Benoit Hardouin; Philippe Moreau; Gilles Potel; Françoise Le Vacon; Marie France de La Cochetière
Source:
Microbial ecology 2014 v.67 no.3 pp. 690-699
ISSN:
0095-3628
Subject:
risk; intestinal microorganisms; sequence analysis; drug therapy; ribosomal RNA; DNA primers; DNA; polymerase chain reaction; high performance liquid chromatography; feces; bone marrow transplant; genes; species diversity; patients; Escherichia; adverse effects
Abstract:
... Gastrointestinal disturbances are a side-effect frequently associated with haematological malignancies due to the intensive cytotoxic treatment given in connection with bone marrow transplantation (BMT). However, intestinal microbiota changes during chemotherapy remain poorly described, probably due to the use of culture-based and low-resolution molecular methods in previous studies. The objective ...
DOI:
10.1007/s00248-013-0355-4
PubMed:
24402367

85. 16S rRNA Gene Sequence Analysis of Drinking Water Using RNA and DNA Extracts as Targets for Clone Library Development

Author:
Randy P. Revetta; Robin S. Matlib; Jorge. W. Santo Domingo
Source:
Current microbiology 2011 v.63 no.1 pp. 50-59
ISSN:
0343-8651
Subject:
Actinobacteria; Cyanobacteria; DNA; DNA libraries; alpha-Proteobacteria; bacteria; beta-Proteobacteria; clones; drinking water; genes; nucleotide sequences; phylogeny; ribosomal RNA; sequence analysis
Abstract:
... The bacterial composition of chlorinated drinking water was analyzed using 16S rRNA gene clone libraries derived from DNA extracts of 12 samples and compared to clone libraries previously generated using RNA extracts from the same samples. Phylogenetic analysis of 761 DNA-based clone sequences showed that unclassified bacteria were the most abundant group, representing nearly 62% of all DNA sequen ...
DOI:
10.1007/s00284-011-9938-9
PubMed:
21533782

86. 16S rRNA Gene and Transcript Profiling: an Application on Full-scale Anaerobic Reactors of Wastewater Sludges

Author:
Deborah Traversi; Caterina Armato; Elisa Anedda; Eugenio Lorenzi; Ilaria Mania; Giorgio Gilli; Roberta Gorra
Source:
BioEnergy research 2019 v.12 no.4 pp. 1134-1144
ISSN:
1939-1234
Subject:
DNA; anaerobic digesters; anaerobic digestion; analysis of variance; biogas; cluster analysis; community structure; denaturing gradient gel electrophoresis; genes; messenger RNA; microbial communities; microorganisms; pollution; ribosomal RNA; sludge; t-test; transcriptomics; wastewater; wastewater treatment
Abstract:
... Sludge anaerobic digestion is a wastewater treatment plant crucial point for the impact mitigation on environment. Such process reduces the disposable sludge volume and pollution potential, generating biogas. Despite its crucial role, sludge digestion microbiology is not clearly understood. In this work, the characterization of the microbiota into digesters fed by primary and secondary sludges was ...
DOI:
10.1007/s12155-019-10012-x

87. 16S rRNA gene amplicon sequencing reveals dominance of Actinobacteria in Rhodnius pallescens compared to Triatoma maculata midgut microbiota in natural populations of vector insects from Colombia

Author:
Luisa M. Montoya-Porras; Triana-Chavez Omar; Juan F. Alzate; Claudia X. Moreno-Herrera; Gloria E. Cadavid-Restrepo
Source:
Acta tropica 2018 v.178 pp. 327-332
ISSN:
0001-706X
Subject:
Aeromonas; Attalea butyracea; Burkholderia; Chagas disease; DNA; Dietzia; Kocuria; Pelomonas; Ralstonia; Rhodnius; Triatoma; Trypanosoma cruzi; aseptic conditions; bacteria; genes; hematophagous insects; high-throughput nucleotide sequencing; microbial communities; midgut; ribosomal RNA; taxonomy; Colombia; Latin America
Abstract:
... Chagas disease affects more than 6 million people in Latin America, it is a parasitic disease caused by the protozoan Trypanosoma cruzi, which is transmitted mainly by bloodsucking insects of the Triatominae subfamily. Studies on microbial communities that inhabit the insect gut are important to understanding their role in the parasite transmission and development. The present work aims to evaluat ...
DOI:
10.1016/j.actatropica.2017.11.004

88. 16S rRNA gene based bacterial community structure of wastewater treatment plant effluents

Author:
Thi Thuy Do; Sarah Delaney; Fiona Walsh
Source:
FEMS microbiology letters 2019 v.366 no.3 pp. -
ISSN:
1574-6968
Subject:
Actinobacteria; Bacteroides; DNA; Firmicutes; Proteobacteria; Tenericutes; Verrucomicrobia; animal pathogens; bacterial communities; community structure; computer software; effluents; genes; human health; humans; microbiome; ribosomal RNA; risk; wastewater treatment
Abstract:
... There is little known about the wastewater treatment plant (WWTP) effluent microbiome, its structure and dynamics. Here, we provide a study of the microbiome of effluent leaving conventional WWTPs and entering the water environment. DNA was extracted from WWTP effluent samples collected in 2015 and 2016. The bacterial communities were studied using Illumina MiSeq 16S rRNA gene amplicon sequencing ...
DOI:
10.1093/femsle/fnz017

89. 16S rRNA gene profiling of planktonic and biofilm microbial populations in the Gulf of Guinea using Illumina NGS

Author:
Sukriye Celikkol-Aydin; Christine C. Gaylarde; Tim Lee; Robert E. Melchers; Devin L. Witt; Iwona B. Beech
Source:
Marine environmental research 2016 v.122 pp. 105-112
ISSN:
0141-1136
Subject:
Bacillariophyceae; DNA; biofilm; carbon; community structure; data analysis; databases; genes; marine environment; oxidants; photosynthesis; plankton; ribosomal RNA; seawater; steel; sulfur; Gulf of Guinea
Abstract:
... 16S rRNA gene profiling using a pipeline involving the Greengenes database revealed that bacterial populations in innermost (proximal to the steel surface) and outer regions of biofilms on carbon steel exposed 3 m below the surface at an offshore site in the Gulf of Guinea differed from one another and from seawater. There was a preponderance of gammaproteobacterial sequences, representing organis ...
DOI:
10.1016/j.marenvres.2016.10.001

90. 16S rRNA-Based metagenomic analysis of microbial communities associated with wild Labroides dimidiatus from Karah Island, Terengganu, Malaysia

Author:
Ashyikin Noor Ahmad Nurul; Danish-Daniel Muhammad; Victor Tosin Okomoda; Ariffin Asma Bt. Nur
Source:
Biotechnology reports 2019 v.21 pp. e00303
ISSN:
2215-017X
Subject:
Actinobacteria; Bacteroidetes; DNA; Firmicutes; Fusobacteria; Labroides dimidiatus; Proteobacteria; bacterial communities; biotechnology; captive animals; corals; metagenomics; microorganisms; mucus; ornamental fish; stomach; Malaysia
Abstract:
... This study was designed to evaluate the bacterial composition of the Labroides dimidiatus and its surrounding water. Fish and carriage water samples were obtained from corals of the Karah Island in Terengganu Malaysia. DNA was extracted and the bacteria communities on the skin mucus and stomach as well as water sample were classified (to family level) using the 16S rRNA-based metagenomics analysis ...
DOI:
10.1016/j.btre.2019.e00303
PubMed:
30671359
PubMed Central:
PMC6328009

91. 16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens

Author:
Muhammad Saeed; Xu Yatao; Zhang Tiantian; Ren Qian; Sun Chao
Source:
Poultry science 2019 v.98 no.2 pp. 842-854
ISSN:
0032-5791
Subject:
Clostridium; DNA; Lactobacillus; Toll-like receptor 4; amino acids; beneficial microorganisms; broiler chickens; chicks; dietary supplements; feed additives; gene expression; green tea; ileum; immune response; interferon-gamma; interleukin-2; intestinal microorganisms; jejunum; messenger RNA; microbiome; ribosomal RNA; sequence analysis; tumor necrosis factor-alpha
Abstract:
... Despite the availability of abundant literature on green tea, studies on the use of L-theanine (an amino acid found only in green tea) as a feed additive in poultry especially broiler are limited. So, this study was conducted to explore the effects of L-theanine on the intestinal microbiome and immune response in a broiler. A total of 400-d-old chicks were randomly divided into four treatment grou ...
DOI:
10.3382/ps/pey394
PubMed:
30169691
PubMed Central:
PMC7107316

92. 16S ribosomal RNA-based gut microbiome composition analysis in infants with breast milk jaundice

Author:
Miao Duan; Jialin Yu; Jinxing Feng; Yu He; Sa Xiao; Danping Zhu; Zhihui Zou
Source:
Open life sciences 2018 v.13 no.1 pp. 208-216
ISSN:
2391-5412
Subject:
Bifidobacterium; DNA; Enterococcus; Escherichia; Shigella; agar gel electrophoresis; bioinformatics; breast feeding; breast milk; case-control studies; digestive system; feces; high-throughput nucleotide sequencing; infants; intestinal microorganisms; jaundice; polymerase chain reaction; ribosomal RNA
Abstract:
... This case-control study investigated an association between breast milk jaundice (BMJ) and infants’ gut microbiome. The study included determination of the diversity of the gut microbiome and identification of bacterial genera associated with BMJ. The study population consisted of 12 infants with BMJ and 22 breastfed infants without jaundice (control). DNA collected from feces was analyzed by PCR ...
DOI:
10.1515/biol-2018-0025

93. 16SrII phytoplasma associated with date palm and Mexican fan palm in Saudi Arabia

Author:
Ayman F. Omar; Abdullah Alsohim; Medhat R. Rehan; Khaled A. Al-Jamhan; Edel Pérez-López
Source:
Australasian plant disease notes 2018 v.13 no.1 pp. 39
ISSN:
1833-928X
Subject:
DNA; Phoenix dactylifera; Phytoplasma; cultivars; financial economics; host plants; phylogeny; Middle East; Saudi Arabia
Abstract:
... Date palm and Mexican fan palm trees showing symptoms previously associated with phytoplasmas were observed in the Al-Qassim region, Saudi Arabia in 2017. DNA amplification, sequencing, and phylogenetic analysis revealed the presence of a ‘Candidatus Phytoplasma australasia’- related strain in eighteen of the eighty-three symptomatic plants collected that were positive using 16S-based assays. This ...
DOI:
10.1007/s13314-018-0326-1

94. 16S‐23S ribosomal RNA spacer regions of Acetobacter europaeus and A. xylinum, tRNA genes and antitermination sequences

Author:
Martin Sievers; Lorenzo Alonso; Sabine Gianotti; Cornelia Boesch; Michael Teuber
Source:
FEMS microbiology letters 1996 v.142 no.1 pp. 43-48
ISSN:
0378-1097
Subject:
Acetobacter; DNA; Gluconacetobacter europaeus; chromosomes; nucleic acid hybridization; nucleotide sequences; nucleotides; operon; polymerase chain reaction; ribosomal RNA; transfer RNA
Abstract:
... The 16S‐23S ribosomal RNA spacer regions of Acetobacter europaeus DSM 6160, A. xylinum NCIB 11664 and A. xyUnion CL27 were amplified by PCR. Specific PCR products were obtained from each strain and their nucleotide sequences determined. The spacer region of A. europaeus comprises 768 nucleotides (nt), that of A. xylinum 778 nt and that of A. xylinum CL27 759 nt. Genes encoding tRNAᴵˡᵉ and tRNAᴬˡᵃ ...
DOI:
10.1111/j.1574-6968.1996.tb08405.x

95. 17 to 23: A novel complementary mini Y‐STR panel to extend the Y‐STR databases from 17 to 23 markers for forensic purposes

Author:
Carolina Núñez; Miriam Baeta; Nerea Ibarbia; Urko Ortueta; Susana Jiménez‐Moreno; José Luis Blazquez‐Caeiro; Juan José Builes; Rene J. Herrera; Begoña Martínez‐Jarreta; Marian M. de Pancorbo
Source:
Electrophoresis 2017 v.38 no.7 pp. 1016-1021
ISSN:
0173-0835
Subject:
DNA; alleles; databases; electrophoresis; forensic sciences; genotyping; haplotypes; loci; males; polymerase chain reaction; traceability
Abstract:
... A Y‐STR multiplex system has been developed with the purpose of complementing the widely used 17 Y‐STR haplotyping (AmpFlSTR Y Filer® PCR Amplification kit) routinely employed in forensic and population genetic studies. This new multiplex system includes six additional STR loci (DYS576, DYS481, DYS549, DYS533, DYS570, and DYS643) to reach the 23 Y‐STR of the PowerPlex® Y23 System. In addition, thi ...
DOI:
10.1002/elps.201600313

96. 170 IN VITRO EMBRYO PRODUCTION AND APOPTOSIS DETECTION USING BOVINE OOCYTES MATURED WITH IGF-I OR IGF-LongR3

Author:
P. L. M. De Chico; E. C. S. Recalde; T. L. Ikeda; M. J. Sudano; F. Landim-Alvarenga
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 215
ISSN:
1031-3613
Subject:
follicle-stimulating hormone; streptomycin; computer software; carbon dioxide; sulfates; fluorescence; DNA; least squares; mineral oil; blastocyst; cytoplasm; embryogenesis; fluorescein; polyvinylpyrrolidone; apoptosis; fetal bovine serum; slaughterhouses; humans; pyruvic acid; cattle; myo-inositol; models; amikacin; insulin; sodium chloride; temperature; oocytes; viability; insulin-like growth factor I; penicillins; polyvinyl alcohol; droplets; luteinizing hormone
Abstract:
... The aim of this study was to compare the differential effects of the addition of IGF-LongR3 or insulin-like growth factor-1 (IGF-1), during in vitro maturation (IVM) of bovine oocytes on embryonic development, by analysing embryonic viability and apoptosis. Ovaries collected at a slaughterhouse were transported in saline solution (NaCl 0.9%) at 38°C. Follicles of 2–8mm of diameter were aspirated. ...
DOI:
10.1071/RDv28n2Ab169

97. 173 Assessment of the first polar body quality and viability in bovine

Author:
O. Briski; M. Duque-Rodríguez; A. Gambini; N. P. Leopardo; E. A. Ynsaurralde; M. B. Rodríguez; R. J. Bevacqua; D. F. Salamone
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 211
ISSN:
1031-3613
Subject:
DNA; DNA fragmentation; analysis of variance; apoptosis; cattle; chromosomes; extrusion; females; fluorescence; meiosis; metaphase; oocytes; plasma membrane; propidium; slaughterhouses; staining; viability
Abstract:
... In female gametes, after the first asymmetric meiotic division, a mature oocyte in metaphase II and a first polar body (PB1) are generated. The PB1 contains one of each pair of homologous chromosomes present in the mature oocyte and its DNA can be used for preconception genetic diagnosis. The PB1 degenerates shortly after extrusion, possibly due to an apoptotic process; however, it has not yet bee ...
DOI:
10.1071/RDv31n1Ab173

98. 174 Follicular fluid extracellular vesicles obtained from Holstein cows kept under thermoneutral or heat stress conditions modify gene expression of in vitro-matured oocytes

Author:
F. M. Dalanezi; R. A. Ferrazza; J. C. Ochoa; H. D. Mogllón; F. C. Destro; F. F. Franchi; P. K. Fontes; A. C. S. Castilho; E. M. S. Schmidt; R. Sartori; J. C. P. Ferreira
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 211-212
ISSN:
1031-3613
Subject:
DNA; Holstein; amikacin; analysis of variance; carbon dioxide; cows; follicle-stimulating hormone; follicular development; follicular fluid; gene expression; genes; glutamine; granulosa cells; heat stress; humidity; meiosis; oocytes; ovulation; progesterone; pyruvic acid; sodium bicarbonate; zebu
Abstract:
... Heat stress (HS) has a massive impact on bovine reproduction. In cows, some of these deleterious effects involve follicular development and oocyte quality. Extracellular vesicles (EV) secreted by granulosa cells play a critical role in the intrafollicular environment by directly influencing cumulus cells and oocyte functions. The objective of this study was to investigate the effect of follicular ...
DOI:
10.1071/RDv31n1Ab174

99. 175 Testis-Specific Protein Y Encoded Copy Number Variation in In Vitro-Produced Bovine Early Embryos

Author:
N.Y. Rho; L. A. Favetta; T. Mogas; W. A. King
Source:
Reproduction, fertility, and development 2018 v.30 no.1 pp. 227
ISSN:
1031-3613
Subject:
DNA; Y chromosome; blastocyst; brothers; bulls; copy number variation; droplets; females; genes; meiosis; polymerase chain reaction; restriction endonucleases; semen; sires; spermatogenesis; zygote
Abstract:
... Testis-specific protein Y (TSPY) is one of the genes located in the male-specific region of the Y chromosome involved in male reproduction, particularly in spermatogenesis. It has been shown to have different copy number (CN) in different species, in different individuals within a species and in the same family (i.e. among brothers from the same father). Previous studies from our laboratory in the ...
DOI:
10.1071/RDv30n1Ab175

100. 179 Decondensation of bovine spermatozoa after dithiothreitol treatment

Author:
L. Gatenby; K. R. Bondioli
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 214
ISSN:
1031-3613
Subject:
DNA; analysis of variance; bulls; centrifugation; disulfide bonds; dithiothreitol; egg yolk; fluorescence microscopy; freeze-thaw cycles; intracytoplasmic sperm injection; pronucleus; semen; spermatozoa
Abstract:
... Dithiothreitol (DTT) has been shown to reduce protamine disulfide bonds present in the outer acrosomal membrane of sperm. These disulfide bonds provide bull sperm with greater membrane stability and an increased resistance to decondensation compared with other species. (Hutchinson et al. 2017 Biophysical J. 113, 1925-1933). This can result in asynchronous fertilization due to the sperm nucleus fai ...
DOI:
10.1071/RDv31n1Ab179