U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject DNA Remove constraint Subject: DNA Subject term rapid methods Remove constraint Subject term: rapid methods
Start Over

Search

1,140  Search Results

« Previous | 1 - 50 of 1,140 |
Number of results to display per page

Search Results

1. Anchoring red blood cell with tetrahedral DNA nanostructure: Electrochemical biosensor for the sensitive signage of circulating tumor DNA

Author:
Xinyue Zha; Weiwei Qin; Jiadi Chen; Mengting Chen; Qiyue Zhang; Kaiyu He; Yingju Liu; Weipeng Liu
Source:
Talanta 2023 v.251 pp. 123793
ISSN:
0039-9140
Subject:
DNA; biocompatibility; biosensors; blood serum; detection limit; erythrocytes; methylene blue; neoplasms; rapid methods
Abstract:
... Circulating tumor cells (CTCs), as a type of tumor, have attracted wide attention because of their characteristics of shedding from the primary tumor and spreading to other tissues and organs through peripheral blood. The circulating tumor DNA (ctDNA), the DNA released by CTCs and other tumor cells into the peripheral blood, was considered as a promising detection substance for clinical applicatio ...
DOI:
10.1016/j.talanta.2022.123793

2. Nondestructive and rapid method for sex identification of the tropical sea cucumber Holothuria scabra by anal swab sampling

Author:
Zixuan E; Chuhang Cheng; Feifei Wu; Chunhua Ren; Rong Chen; Yingzhu Rao; Bo Ma; Xiao Jiang; Peng Luo; Xiaomin Li; Xin Zhang; Fajun Jiang; Chaoqun Hu; Ting Chen
Source:
Aquaculture 2023 v.562 pp. 738749
ISSN:
0044-8486
Subject:
DNA; Holothuria scabra; aquaculture; dimorphism; females; genomics; loop-mediated isothermal amplification; males; phenotype; rapid methods; reproduction; South East Asia
Abstract:
... As one of the most economically significant sea cucumber species, Holothuria scabra are naturally distributed in the Indo-West Pacific region and are widely cultural in Southeast Asia. The appearances of female and male H. scabra have no obvious phenotypic dimorphism. Thus, identification of sex-specific markers and establishment of sex detection methods are beneficial to artificially induced repr ...
DOI:
10.1016/j.aquaculture.2022.738749

3. Accelerated cycling PCR: A novel tool for rapid, sensitive and specific detection of single-nucleotide mutation within 30 min

Author:
Zhixian Luan; Yan Zhao; Yanling Wang; Cuiping Ma; Chao Shi
Source:
Journal of microbiological methods 2022 v.199 pp. 106527
ISSN:
0167-7012
Subject:
DNA; Helicobacter pylori; drug resistance; genotype; genotyping; mutation; rapid methods
Abstract:
... Rapid detection of single-nucleotide mutations (SNMs) has played a vital role for point-of-care testing. We herein first introduced accelerated thermal cycling into conventional allele-specific qPCR (AS-qPCR), named accelerated cycling PCR (AC-PCR) to achieve rapid and sensitive detection of SNM. It could simultaneously detect 10 copies of H. pylori DNA and identify its clarithromycin-resistance g ...
DOI:
10.1016/j.mimet.2022.106527

4. Alkaline lysis-recombinase polymerase amplification combined with CRISPR/Cas12a assay for the ultrafast visual identification of pork in meat products

Author:
Gang Zhao; Jin Wang; Chanyu Yao; Peichun Xie; Xiangmei Li; Zhenlin Xu; Yanping Xian; Hongtao Lei; Xing Shen
Source:
Food chemistry 2022 v.383 pp. 132318
ISSN:
0308-8146
Subject:
DNA; beef; detection limit; equipment; food chemistry; human resources; pork; quantitative polymerase chain reaction; rapid methods; raw meat; recombinase polymerase amplification; swine
Abstract:
... The “Horsemeat Scandal” makes people pay more attention to the meat authenticity. However, expensive equipment, complicated operations, and professional personnel of current methods limit their field testing. In this study, CRISPR/Cas12a combined with recombinase polymerase amplification was used to establish a sensitive and rapid detection method for pig-derived component. The detection limit can ...
DOI:
10.1016/j.foodchem.2022.132318

5. Assessing the impact of the routine childhood hepatitis B immunization program and the need for hepatitis B vaccine birth dose in Sierra Leone, 2018

Author:
Lucy Breakwell; Dennis Marke; Reinhard Kaiser; Alexandra Tejada-Strop; Matthew D. Pauly; Sonnia Jabbi; Sahr Yambasu; Hyacinte J. Kabore; Brock Stewart; Tom Sesay; Thomas T. Samba; Tonya Hayden; Saleem Kamili; Amara Jambai; Jan Drobeniuc; Tushar Singh; Rania A. Tohme; Annemarie Wasley
Source:
Vaccine 2022 v.40 no.19 pp. 2741-2748
ISSN:
0264-410X
Subject:
DNA; Hepatitis B virus; childhood; hepatitis B; rapid methods; risk; surface antigens; surveys; vaccination; vaccines; vertical disease transmission; Sierra Leone
Abstract:
... Sierra Leone is highly endemic for hepatitis B virus (HBV) infection and thus recommends three doses of hepatitis B vaccine (HepB3) from 6 weeks of age but does not recommend a birth dose (HepB-BD) to prevent mother-to-child transmission (MTCT). We evaluated impact of the existing HepB3 schedule and risk for MTCT of HBV. We conducted a community-based serosurvey among 4–30-month-olds, their mother ...
DOI:
10.1016/j.vaccine.2022.03.049

6. Biosensor prototype for rapid detection and quantification of DNase activity

Author:
Skomantas Serapinas; Justina Gineitytė; Marius Butkevičius; Rapolas Danilevičius; Marius Dagys; Dalius Ratautas
Source:
Biosensors & bioelectronics 2022 v.213 pp. 114475
ISSN:
0956-5663
Subject:
DNA; algorithms; biosensors; biotechnology; blood; deoxyribonucleases; detection limit; diagnostic techniques; electrodes; electronics; humans; industry; prototypes; quality control; rapid methods; saliva; sweat
Abstract:
... DNases are enzymes that cleave phosphodiesteric bonds of deoxyribonucleic acid molecules and are found everywhere in nature, especially in bodily fluids, i.e., saliva, blood, or sweat. Rapid and sensitive detection of DNase activity is highly important for quality control in the pharmaceutical and biotechnology industries. For clinical diagnostics, recent reports indicate that increased DNase acti ...
DOI:
10.1016/j.bios.2022.114475

7. CE–RAA–CRISPR Assay: A Rapid and Sensitive Method for Detecting Vibrio parahaemolyticus in Seafood

Author:
Xinrui Lv; Weiwei Cao; Huang Zhang; Yilin Zhang; Lei Shi; Lei Ye
Source:
Foods 2022 v.11 no.12 pp. -
ISSN:
2304-8158
Subject:
CRISPR-associated proteins; DNA; Vibrio parahaemolyticus; aerosols; bovine serum albumin; cross reaction; detection limit; food safety; mariculture; proline; quantitative polymerase chain reaction; rapid methods; risk; seafoods; shrimp
Abstract:
... Vibrio parahaemolyticus is one of the major pathogenic Vibrio species that contaminate seafood. Rapid and accurate detection is crucial for avoiding foodborne diseases caused by pathogens and is important for food safety management and mariculture. In this study, we established a system that combines chemically enhanced clustered regularly interspaced short palindromic repeats (CRISPR) and recombi ...
DOI:
10.3390/foods11121681

8. Characterization of the O-antigen gene clusters and development of a molecular serotyping method for Vibrio fluvialis

Author:
Min Wang; Bin Liu; Yanli Xu; Xi Guo; Tian Fu; Jiafei Liu; Lei Wang
Source:
International journal of food microbiology 2022 v.370 pp. 109665
ISSN:
0168-1605
Subject:
DNA; O-antigens; Vibrio fluvialis; antigenic variation; detection limit; food pathogens; food safety; monitoring; multigene family; oysters; rapid methods; serotypes
Abstract:
... Vibrio fluvialis is an emerging foodborne pathogen that causes severe infections. Serotyping based on surface polysaccharide antigens is important for the clinical detection and epidemiological surveillance of pathogens such as V. fluvialis. For example, variation of the O-antigen, which is highly polymorphic and is responsible for the majority of antigenic variability on the bacterial cell surfac ...
DOI:
10.1016/j.ijfoodmicro.2022.109665

9. A Combination of Novel Nucleic Acid Cross-Linking Dye and Recombinase-Aided Amplification for the Rapid Detection of Viable Salmonella in Milk

Author:
Xiaoyan Feng; Donggen Zhou; Bei Gan; Guoyang Xie; Hengyi Xu
Source:
Foods 2022 v.11 no.15 pp. -
ISSN:
2304-8158
Subject:
DNA; Salmonella; biochemical pathways; cell membranes; crosslinking; detection limit; dyes; enteritis; fluorescence; food pathogens; milk; permeability; rapid methods; spectroscopy; thiazoles
Abstract:
... Salmonella, as an important foodborne pathogen, can cause various diseases, such as severe enteritis. In recent years, various types of nucleicacid-intercalating dyes have been utilized to detect viable Salmonella. However, in principle, the performance of existing nucleic acid dyes is limited because they depend on the integrity of cell membrane. Herein, based on the metabolic activity of bacteri ...
DOI:
10.3390/foods11152375

10. Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection

Author:
Han-Yun Hsieh; Ray Chang; Yung-Yu Huang; Po-Han Juan; Hidetoshi Tahara; Kuan-Yi Lee; Di Ngoc Kha Vo; Ming-Han Tsai; Pei-Kuen Wei; Horn-Jiunn Sheen; Yu-Jui Fan
Source:
Biosensors & bioelectronics 2022 v.195 pp. 113672
ISSN:
0956-5663
Subject:
COVID-19 infection; DNA; DNA probes; Human gammaherpesvirus 4; biosensors; calibration; detection limit; genes; gold; membrane proteins; microfluidic technology; nucleotide sequences; polymerase chain reaction; rapid methods; surface plasmon resonance
Abstract:
... We present the first combination of a microfluidic polymerase chain reaction (PCR) with a gold nanoslit-based surface plasmon resonance (SPR) sensor for detecting the DNA sequence of latent membrane protein 1 (LMP1). The PCR microchannel was produced through a laser scribing technique, and the SPR nanoslit chip was manufactured via hot-embossing nanoimprinting lithography. Afterward, the LMP1 DNA ...
DOI:
10.1016/j.bios.2021.113672

11. Determination of mating types of Fulvia fulva isolates from Turkey

Author:
Esra Gül; Aziz Karakaya; Ali Ergül
Source:
Tropical plant pathology 2022 v.47 no.3 pp. 421-429
ISSN:
1983-2052
Subject:
DNA; Passalora fulva; enzymes; fungi; greenhouse production; greenhouses; hydroponics; leaves; microbial detection; pathogens; plant pathology; polymerase chain reaction; probability; rapid methods; sexual reproduction; tomatoes; tropical plants
Abstract:
... Tomato is among the most produced and exported products in greenhouse cultivation in Turkey. Tomato leaf mold is one of the most important fungal diseases that can cause significant yield losses in greenhouse and hydroponic tomato cultivation. In this study, the mating types of 97 Fulvia fulva isolates obtained from different provinces in Turkey were determined. We found that 43 isolates were MAT1 ...
DOI:
10.1007/s40858-021-00482-w

12. Development and Comparison of Seminested PCR, qPCR, and LAMP for the Rapid Detection of Arthrinium phaeospermum, the Causal Agent of Bamboo Blight

Author:
Han Zhang; Weiyi Yang; Liling Xie; Tianhui Zhu; Shuying Li; Shan Han; Tiantian Lin; Shujiang Li
Source:
Forests 2022 v.13 no.6 pp. -
ISSN:
1999-4907
Subject:
Bambusa pervariabilis; DNA; bamboos; blight; early diagnosis; financial economics; hybrids; quantitative polymerase chain reaction; rapid methods
Abstract:
... Bambusa pervariabilis × Dendrocalamopsis grandis blight is a newly discovered disease in bamboos that has caused substantial economic loss to the affected areas. With the purpose of carrying out rapid detection of Bambusa pervariabilis × Dendrocalamopsis grandis blight caused by Arthrinium phaeospermum during the incubation period, three sets of detection assays were established: seminested PCR, r ...
DOI:
10.3390/f13060850

13. Development and Evaluation of a TaqMan Real-Time PCR Assay for the Rapid Detection of Cross-Contamination of RD (Human) and L20B (Mouse) Cell Lines Used in Poliovirus Surveillance

Author:
Ausaf Ahmad; Joo R. Lee; John M. Metz; Xiaoling Tang; Seh-ching Lin; Dennis A. Bagarozzi; David Petway; Owen Herzegh
Source:
Journal of virological methods 2022 v.300 pp. 114354
ISSN:
0166-0934
Subject:
DNA; DNA barcoding; Enterovirus C; cross contamination; cross reaction; detection limit; diagnostic techniques; genes; humans; mice; mitochondria; monitoring; monkeys; public health; quantitative polymerase chain reaction; rapid methods; rats
Abstract:
... The cross-contamination of cell lines in culture is a persistent problem. Genetically modified L20B (Mouse) and RD (Human Rhabdomyosarcoma) cell lines are commonly used in poliovirus research, surveillance, and diagnostics. Cross-contamination between these cell lines leads to unreproducible results and unreliable surveillance data, negatively affecting public health. The gold standard method for ...
DOI:
10.1016/j.jviromet.2021.114354

14. Development and application of a multiplex PCR method to differentiate Salmonella enterica serovar Typhimurium from its monophasic variants in pig farms

Author:
Yaming Hong; Ruoyun Ji; Zhenyu Wang; Jiaojie Gu; Xinan Jiao; Qiuchun Li
Source:
Food microbiology 2023 v.109 pp. 104135
ISSN:
0740-0020
Subject:
DNA; Salmonella Typhimurium; detection limit; farms; flagellin; food microbiology; food production; genes; humans; polymerase chain reaction; rapid methods; salmonellosis; serotypes; swine
Abstract:
... Salmonella enterica serovar Typhimurium monophasic variants (Salmonella 4,[5],12:i:-) has increased dramatically, causing human salmonellosis and colonization in pigs. With a difference to S. Typhimurium, the monophasic variants of S. Typhimurium lose the gene cassettes encoding the second phase flagellin. To establish a rapid method to detect and differentiate the two serotypes, we analyzed the p ...
DOI:
10.1016/j.fm.2022.104135

15. Development and evaluation of a novel visual and rapid detection assay for toxigenic Fusarium graminearum in maize based on recombinase polymerase amplification and lateral flow analysis

Author:
Xiaoyan Liang; Xiu Zhang; Hafiz Abdul Haseeb; Tingting Tang; Jihao Shan; Bo Yin; Wei Guo
Source:
International journal of food microbiology 2022 v.372 pp. 109682
ISSN:
0168-1605
Subject:
DNA; Fusarium graminearum; animal and human health; corn; corn ears; cost effectiveness; deoxynivalenol; ear rot; equipment; food microbiology; galactose oxidase; genes; ingestion; nivalenol; polymerase chain reaction; rapid methods; recombinase polymerase amplification; risk; zearalenone
Abstract:
... Maize ear rot caused by Fusarium graminearum is one of the most severe maize diseases in global maize-growing regions. It reduces maize yield in the field and is also responsible for mycotoxin contamination of grains during the postharvest period. F. graminearum is one of the major deoxynivalenol (DON), nivalenol (NIV), and zearalenone (ZEN) producers. The ingestion of these mycotoxins represents ...
DOI:
10.1016/j.ijfoodmicro.2022.109682

16. Development and evaluation of an isothermal recombinase polymerase amplification–lateral flow assay for rapid detection of strawberry vein banding virus in the field

Author:
Xiaohua Zou; Shidong Yuan; Chao Dong; Qinghua Gao
Source:
Crop protection 2022 v.158 pp. 105994
ISSN:
0261-2194
Subject:
DNA; detection limit; plant protection; rapid methods; recombinases; strawberries; viruses; China
Abstract:
... The disease caused by strawberry vein banding virus (SVBV) is one of the main virus diseases affecting the growth and development of strawberries, and the development of an on-site method for rapid detection of SVBV would be useful for breeding virus-free strawberry seedlings. In this study, we used isothermal recombinant polymerase amplification (RPA) combined with lateral flow strip (LF strip) t ...
DOI:
10.1016/j.cropro.2022.105994

17. Development and validation of an insulated isothermal PCR assay for the rapid detection of Mannheimia haemolytica

Author:
Xiaofang Dao; Chien-Che Hung; Youwen Yang; Juan Wang; Falong Yang
Source:
Journal of veterinary diagnostic investigation 2022 v.34 no.2 pp. 302-305
ISSN:
1943-4936
Subject:
Arcanobacterium pyogenes; DNA; Haemophilus somni; Mannheimia haemolytica; Mycoplasma; Streptococcus suis; bacterial culture; cattle; genes; portable equipment; rapid methods; respiratory tract diseases; sheep; superoxide dismutase
Abstract:
... We developed a rapid insulated isothermal PCR (iiPCR) assay for on-site detection of Mannheimia haemolytica using a primer and probe set targeting the superoxide dismutase (sodA) gene. Our iiPCR assay detected M. haemolytica clinical isolates successfully and produced negative results on other bovine or ovine respiratory pathogens, including Histophilus somni, Bibersteinia trehalosi, Trueperella p ...
DOI:
10.1177/10406387211068447

18. Development of Loop-Mediated Isothermal Amplification Assay for Rapid Detection and Analysis of the Root-Knot Nematode Meloidogyne hapla in Soil

Author:
Zahra Saad Omer; Ann-Charlotte Wallenhammar; Maria Viketoft
Source:
Horticulturae 2022 v.8 no.2 pp. -
ISSN:
2311-7524
Subject:
DNA; Meloidogyne hapla; colorimetry; crop damage; detection limit; farms; genes; loop-mediated isothermal amplification; rapid methods; root-knot nematodes; soil
Abstract:
... Soil analysis is crucial for estimating the risk of crop damage by the root-knot nematode Meloidogyne hapla. Here, we developed an analysis assay based on Loop-mediated Isothermal Amplification (LAMP). The LAMP primers were verified for specificity against 10 different nematode species. A manual soil DNA extraction, referred to as SKMM, was developed and compared with a FastDNA kit followed by DNA ...
DOI:
10.3390/horticulturae8020087

19. Development of gold nanoparticle-based visual assay for rapid detection of Escherichia coli specific DNA in milk of cows affected with mastitis

Author:
Rajib Deb; Prasanna Pal; Parul Chaudhary; Sanat Bhadsavle; Manisha Behera; Parmanand; Devika Gautam; Mayank Roshan; Ashutosh Vats; Ashutosh Ludri; Vivek Kumar Gupta; Sachinandan De
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.155 pp. 112901
ISSN:
0023-6438
Subject:
DNA; Escherichia coli; beta-glucuronidase; early diagnosis; equipment; genes; gold; magnetism; microbeads; microbial load; milk; nanogold; rapid methods
Abstract:
... For early diagnosis of infections, a rapid, sensitive and precise method to identify microbial DNA coming from veterinary clinical samples, such as milk of cows affected with mastitis, is fascinating. We developed an amplification-free visual assay for rapid and sensitive detection of specific DNA from Escherichia coli, particularly uid A gene encoding for beta-glucuronidase, isolated in milk of c ...
DOI:
10.1016/j.lwt.2021.112901

20. Development of loop mediated isothermal amplification for rapid species detection of Armillaria ostoyae using assimilating probe

Author:
Tomáš Tonka; Dagmar Stehlíková; Lucie Walterová; Vladislav Čurn
Source:
Journal of Forest Science v.68 no.5 pp. 163-169
ISSN:
1212-4834
Subject:
Armillaria gallica; Armillaria ostoyae; DNA; Palearctic region; detection limit; fluorescence; fluorescent dyes; forests; loop-mediated isothermal amplification; oligonucleotides; peptide elongation factors; portable equipment; rapid methods
Abstract:
... We introduced here the first loop mediated isothermal amplification (LAMP) assay for the identification of honey fungus, Armillaria ostoyae, a basidiomycote playing an important role in spruce declines in the Palaearctic region. In total, 101 isolates, representing three Armillaria species, were used to develop a new LAMP assay to determine species specific identification. We have here described L ...
DOI:
10.17221/161/2021-jfs

21. Diagnostics of the peach root-knot nematode, Meloidogyne floridensis using multiplex real-time PCR

Author:
Henok Yimer; Julie Burbridge; Janete A. Brito; Steven A. Nadler; John J. Chitambar; Sergei A. Subbotin
Source:
European journal of plant pathology 2022 v.164 no.1 pp. 109-123
ISSN:
0929-1873
Subject:
DNA; Meloidogyne floridensis; data collection; diagnostic techniques; fluorescence; juveniles; mitochondria; morphometry; nucleotide sequences; peaches; plant pathology; quantitative polymerase chain reaction; rapid methods; root-knot nematodes; California; Florida; Georgia; Mexico; South Carolina
Abstract:
... The peach root-knot nematode, Meloidogyne floridensis is an emerging pest of peach and other crops that is currently known to occur only in California, Florida, Georgia and South Carolina, USA. Morphological and morphometric differentiation of this species from other related species is difficult and requires taxonomic expertise. Molecular methods can provide rapid and reliable diagnostics of M. fl ...
DOI:
10.1007/s10658-022-02542-6

22. An Efficient Droplet Digital PCR Approach for Detection DNA at Low Concentrations of Toxigenic Fungi in Food Products

Author:
Ping Wang; Tianming Qu; Xiaomei Zhao; Yiqiang Ge; Ying Chen
Source:
Food analytical methods 2022 v.15 no.6 pp. 1695-1706
ISSN:
1936-9751
Subject:
Aspergillus; DNA; Fusarium; Penicillium; aflatoxins; carcinogenicity; detection limit; droplets; fluorescence; food safety; genes; hepatotoxicity; humans; rapid methods; teratogenicity
Abstract:
... As common toxigenic fungi genera, Aspergillus, Penicillium, and Fusarium, have attracted the attention of governments and people all over the world due to their human carcinogenicity, teratogenicity, and hepatotoxicity. Accordingly, effective and quantitative detection method for toxigenic fungi before mycotoxins produced should be established to ensure food safety. In this paper, two duplex-dropl ...
DOI:
10.1007/s12161-022-02236-z

23. Electrochemical Aptamer Biosensor Based on ATP-Induced 2D DNA Structure Switching for Rapid and Ultrasensitive Detection of ATP

Author:
Ying Liu; Lingqi Kong; Hao Li; Ruo Yuan; Yaqin Chai
Source:
Analytical chemistry 2022 v.94 no.18 pp. 6819-6826
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; biosensors; detection limit; disease diagnosis; electrochemistry; electrodes; methylene blue; oligonucleotides; rapid methods
Abstract:
... Herein, a two-dimensional (2D) DNA structure with multiple ATP aptamers was elegantly designed to establish an electrochemical biosensor for rapid and sensitive detection of ATP based on ATP-induced structure switching. Concretely, the prepared 2D DNA structure containing numerous ATP aptamers as ATP-specific toehold switches could not only immobilize a large number of methylene blue (MB) for gene ...
DOI:
10.1021/acs.analchem.2c00613

24. Engineering DNAzyme strategies for fluorescent detection of lead ions based on RNA cleavage-propelled signal amplification

Author:
Ying Li; Kai Liu; Boxu Wang; Zheng Liu; Chuanyu Yang; Junyang Wang; Xinyue Ma; Hongxia Li; Chunyan Sun
Source:
Journal of hazardous materials 2022 v.440 pp. 129712
ISSN:
0304-3894
Subject:
DNA; RNA; adsorption; biosensors; detection limit; digestion; fluorescence; lead; pollution; rapid methods
Abstract:
... Based on the high recognition ability and flexible programmability of GR5 DNAzyme, two fluorescent biosensors were engineered for amplified detection of Pb²⁺ via incorporating Ti₃C₂TX MXenes and embedding 2-aminopurine (2-AP), respectively. The quencher-required approach relied on the DNA affinity and fluorescence quenching ability of Ti₃C₂TX MXenes. Benefiting from the low background signal modul ...
DOI:
10.1016/j.jhazmat.2022.129712

25. Establishment of a rapid method for skipjack tuna (Katsuwonus pelamis) authentication using molecular beacons in loop-mediated isothermal amplification

Author:
Wenjie Xu; Qiuping Li; Hanyue Xue; Yanjin Fei; Xiaowen Cui; Min Cao; Xiaohui Xiong; Xiong Xiong; Ying Yang; Libin Wang
Source:
Food chemistry 2022 v.382 pp. 132365
ISSN:
0308-8146
Subject:
DNA; Katsuwonus pelamis; case studies; fluorescence; fluorescent dyes; food chemistry; loop-mediated isothermal amplification; nucleobases; rapid methods
Abstract:
... One major drawback to the traditional loop-mediated isothermal amplification (LAMP) detection methods is the increased likelihood of detecting false-positive signals derived from non-specific amplification. Molecular beacon (MB) is increasingly being used in many applications and the MB-LAMP assay has proved itself as a target-specific method. The present work selected skipjack tuna as a case stud ...
DOI:
10.1016/j.foodchem.2022.132365

26. HRM analysis enables efficient detection and differentiation of two boxwood blight pathogens by qPCR assays

Author:
Gouker Fred Edward; Guo Yonghong Henry; Pooler Margaret R.
Source:
PhytoFrontiers 2022 v. no. pp. -
ISSN:
2690-5442
Subject:
Buxus sempervirens; Calonectria henricotiae; Calonectria pseudonaviculata; DNA; boxwood blight; calmodulin; cultivars; disease control; fungi; histones; industry; pathogens; plant tissues; quantitative polymerase chain reaction; rapid methods; temperature
Abstract:
... Boxwood blight is a devastating disease caused by fungal pathogens Calonectria pseudonaviculata (Cps) and Calonectria henricotiae (Che). Identification and detection of this pathogen from infected plant material could play a significant role in breeding and selection of resistant cultivars and development of disease management strategies in the ornamental nursery industry. We designed a simple, si ...
Handle:
10113/7800860
DOI:
10.1094/PHYTOFR-09-21-0066-SC

27. Identification of Male-Specific Molecular Markers by Recombination of RhoGEF10 Gene in Spotted Knifejaw (Oplegnathus punctatus)

Author:
Yanduo Wu; Yongshuang Xiao; Zhizhong Xiao; Yuting Ma; Haixia Zhao; Jun Li
Source:
Genes 2022 v.13 no.7 pp. -
ISSN:
2073-4425
Subject:
DNA; DNA fragmentation; Oplegnathus; Y chromosome; dimorphism; ecological value; females; fish; genes; genomics; males; myelin sheath; nerve tissue; nutritive value; rapid methods; sex determination
Abstract:
... The spotted knifejaw (Oplegnathus punctatus) is a marine economic fish with high ecological value, food value, and fishing value, and its growth has obvious sex dimorphism. The rapid identification of its sex is beneficial to the development of sex determination and breeding. In this study, the method of comparative genomics and PCR amplification was used to further establish a rapid detection met ...
DOI:
10.3390/genes13071262

28. Label-Free Fluorescence Sensing Strategy Based on Functional Nucleic Acids via Energy Transfer between DNA-Templated Silver Nanoclusters and Gold Nanorods

Author:
Junyang Wang; Qian Zhang; Zheng Liu; Chuanyu Yang; Ying Li; Xinyue Ma; Ting Lv; Chunyan Sun
Source:
Journal of agricultural and food chemistry 2022 v.70 no.38 pp. 12220-12231
ISSN:
1520-5118
Subject:
DNA; chemical species; energy transfer; enzymes; fluorescence; food chemistry; food contamination; gold; heavy metals; milk; nanorods; nanosilver; oligonucleotides; rapid methods; river water; tetracycline
Abstract:
... A simple and low-cost fluorescence signal-on sensing strategy has been developed based on functional nucleic acids (FNAs) via energy transfer between DNA-templated silver nanoclusters (DNA-AgNCs) and gold nanorods (GNRs). FNAs were used as highly selective recognition probes, in which an aptamer was used to detect small molecules represented by tetracycline, and DNAzyme was used to detect heavy me ...
DOI:
10.1021/acs.jafc.2c05143

29. Label-free fluorescence aptasensor for the detection of patulin using target-induced DNA gates and TCPP/BDC-NH2 mixed ligands functionalized Zr-MOF systems

Author:
Xiaohai Yan; Gengan Du; Hong Chen; Qiannan Zhao; Qi Guo; Jianlong Wang; Zhouli Wang; Wei Song; Qinglin Sheng; Yane Luo; Yahong Yuan; Tianli Yue
Source:
Biosensors & bioelectronics 2022 v.217 pp. 114723
ISSN:
0956-5663
Subject:
Aspergillus clavatus; DNA; Penicillium expansum; apple juice; aptasensors; detection limit; fluorescence; food safety; ligands; oligonucleotides; patulin; porosity; rapid methods
Abstract:
... Patulin (PAT) is an unsaturated lactone mycotoxin primarily produced by Penicillium expansum and Aspergillus clavatus. Given the potential health risks and economic losses associated with PAT, the rapid detection of PAT using fluorescent aptasensors is of significant importance in evaluating food safety. However, it easily increases the cost and complexity caused by signal labeling. We combined TC ...
DOI:
10.1016/j.bios.2022.114723

30. Loop-Mediated Isothermal Amplification for Rapid Detection of Mating Types of Villosiclava virens

Author:
Xiayan Pan; Xiao Wang; Junjie Yu; Mina Yu; Huijuan Cao; Mingli Yong; Tianqiao Song; Zhongqiang Qi; Yan Du; Rongsheng Zhang; Yongfeng Liu
Source:
Plant disease 2022 v.106 no.4 pp. 1128-1133
ISSN:
0191-2917
Subject:
DNA; Ustilaginoidea virens; fungi; loop-mediated isothermal amplification; rapid methods; rice; rice false smut; sexual reproduction
Abstract:
... Rice false smut (RFS), caused by Villosiclava virens, is an important fungal disease in panicles of rice. V. virens is a heterothallic ascomycete controlled by two opposite idiomorphs, MAT1-1 and MAT1-2. Previous study showed that sexual reproduction of V. virens plays an important role in the epidemic of RFS. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay to det ...
DOI:
10.1094/PDIS-09-21-1943-RE

31. Modern on-site tool for monitoring contamination of halal meat with products from five non-halal animals using multiplex polymerase chain reaction coupled with DNA strip

Author:
Anat Denyingyhot; Theerarak Srinulgray; Pornpimol Mahamad; Arnakorn Ruangprach; Suwainah Sa-I; Tuanyasmin Saerae; Mongkol Vesaratchavest; Winai Dahlan; Suwimon Keeratipibul
Source:
Food control 2022 v.132 pp. 108540
ISSN:
0956-7135
Subject:
DNA; adulterated products; beef; biotin; detection limit; food industry; food safety; halal foods; mitochondria; polymerase chain reaction; rapid methods; swine; Thailand
Abstract:
... Meat adulteration is a persistent problem in the food industry in many countries. This unfair practice can potentially not only affect the economy of a country but also the confidence and health of its citizens. Additionally, it can aggravate allergies or damage religious beliefs (e.g., Muslims). The aim of this study was to develop a simple and rapid technique for the on-site inspection of severa ...
DOI:
10.1016/j.foodcont.2021.108540

32. Molecular Recognition-Triggered Aptazyme Sensor Using a Co-MOF@MCA Hybrid Nanostructure as Signal Labels for Adenosine Triphosphate Detection in Food Samples

Author:
Ruina Zheng; Baoshan He; Lingling Xie; Haoyang Yan; Liying Jiang; Wenjie Ren; Zhiguang Suo; Yiwei Xu; Min Wei; Huali Jin
Source:
Analytical chemistry 2022 v.94 no.37 pp. 12866-12874
ISSN:
1520-6882
Subject:
DNA; adenosine triphosphate; analytical chemistry; aptasensors; coordination polymers; detection limit; electrical conductivity; electrochemistry; food safety; melamine; methylene blue; molybdenum disulfide; nanomaterials; rapid methods; surface area
Abstract:
... Developing rapid detection technology for adenosine triphosphate (ATP) is crucial in quality supervision and food safety. Herein, an electrochemical aptasensor based on an aptazyme-catalyzed signal amplification strategy is constructed for ATP detection using polyethyleneimine-functionalized molybdenum disulfide (PEI-MoS₂)/Au@PtPd nanobipyramids (MoS₂/Au@PtPd NBPs) as a modification material. Addi ...
DOI:
10.1021/acs.analchem.2c02916

33. Molecular diagnosis and direct quantification of cereal cyst nematode (Heterodera filipjevi) from field soil using TaqMan real-time PCR1

Author:
Jin-zhuo JIAN; Wen-kun HUANG; Ling-an KONG; Heng JIAN; Sulaiman ABDULSALAM; De-liang PENG; Huan PENG
Source:
Journal of integrative agriculture 2022 pp. -
ISSN:
2095-3119
Subject:
DNA; Globodera; Heterodera filipjevi; Pratylenchus; agriculture; cyst nematodes; detection limit; females; fluorescence; genomics; quantitative polymerase chain reaction; rapid methods; soil; wheat
Abstract:
... Heterodera filipjevi continues to be a major threat to wheat production worldwide. Rapid detection and quantification of cyst nematodes are essential for more effective control against this nematode disease. In the present study, a TaqMan-minor groove binder (TaqMan-MGB) probe-based fluorescence quantitative real-time polymerase chain reaction (qPCR) was successfully developed and used for quantif ...
DOI:
10.1016/j.jia.2022.09.016

34. Mpl-Gene-Based Loop-Mediated Isothermal Amplification Assay for Specific and Rapid Detection of Listeria monocytogenes in Various Food Samples

Author:
Annemarie Busch; André Becker; Ulrich Schotte; Madeleine Plötz; Amir Abdulmawjood
Source:
Foodborne pathogens & disease 2022 v.19 no.7 pp. 463-472
ISSN:
1556-7125
Subject:
DNA; European Union; Listeria monocytogenes; bacterial contamination; detection limit; food safety; listeriosis; loop-mediated isothermal amplification; minced beef; quantitative polymerase chain reaction; rapid methods; risk; test meals
Abstract:
... Listeria monocytogenes represents a high risk in food and can trigger potentially fatal listeriosis. The objective of this study was to detect L. monocytogenes in food using the LAMP method in a fast, specific, sensitive manner and thus to preventively test food for the presence of the target species. The reaction was performed and established using the portable real-time fluorometer Genie® II (Op ...
DOI:
10.1089/fpd.2021.0080

35. Multiplexed miRNA Quantitation Using Injectionless Microfluidic Thermal Gel Electrophoresis

Author:
Mario A. Cornejo; Thomas H. Linz
Source:
Analytical chemistry 2022 v.94 no.14 pp. 5674-5681
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; automation; electric potential difference; fluorescence; gel electrophoresis; gels; geometry; microRNA; protein synthesis; rapid methods
Abstract:
... MicroRNAs (miRNAs) are a class of biomolecules that have high clinical and pharmaceutical significance because of their ability to regulate protein expression. Better methods are needed to quantify target miRNAs, but their similar sequence lengths and low concentrations in biomedical samples impede analysis. This report aimed to develop a simple, rapid method to directly quantify multiple miRNAs u ...
DOI:
10.1021/acs.analchem.2c00356

36. One Versatile Cas9-Integrated Single-Tube Duplex Quantitative Real-Time PCR System for Rapid Analysis of CRISPR/Cas-Induced Mutants

Author:
Xueqi Li; Rong Li; Zheng Yuan; Zaobing Zhu; Wenting Xu; Yijie Wang; Dabing Zhang; Litao Yang
Source:
Analytical chemistry 2022 v.94 no.30 pp. 10832-10840
ISSN:
1520-6882
Subject:
CRISPR-Cas systems; DNA; Oryza sativa; RNA; analytical chemistry; animals; gene editing; genes; genotyping; heterozygosity; homozygosity; loci; mutants; progeny; quantitative polymerase chain reaction; rapid methods; rice
Abstract:
... Clustered regularly interspersed short palindromic repeat (CRISPR)/Cas9 gene editing has become a common tool for rapid crop and animal breeding, but efficiently screening out and genotyping for the CRISPR/Cas9-induced mutant lines at a low cost remains challenging. Using rice (Oryza sativa L.) samples genetically edited at the Waxy locus as an example, we developed a single-tube duplex quantitati ...
DOI:
10.1021/acs.analchem.2c01837

37. PMAxx Combined with Recombinase Aided Amplification Technique for Specific and Rapid Detection of Salmonella in Milk

Author:
Jingbin Li; Donggen Zhou; Guoyang Xie; Mei Deng; Xiaoyan Feng; Hengyi Xu
Source:
Food analytical methods 2022 v.15 no.7 pp. 1769-1777
ISSN:
1936-9751
Subject:
DNA; Salmonella; detection limit; genes; milk; propidium; rapid methods; recombinases
Abstract:
... In this study, a recombinase aided amplification (RAA) assay with an improved propidium monoazide (PMAxx) treatment called PMAxx-RAA assay was developed to detect Salmonella in milk. The established method was performed at 39 °C and the detection time was less than 40 min. Compared with traditional detection methods, the PMAxx-RAA assay is fast, sensitive, and specific because of the gene invA tha ...
DOI:
10.1007/s12161-022-02249-8

38. PhageLeads: Rapid Assessment of Phage Therapeutic Suitability Using an Ensemble Machine Learning Approach

Author:
Kumarasan Yukgehnaish; Heera Rajandas; Sivachandran Parimannan; Ravichandran Manickam; Kasi Marimuthu; Bent Petersen; Martha R. J. Clokie; Andrew Millard; Thomas Sicheritz-Pontén
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
DNA; antibiotic resistance; bacteriophages; computer simulation; immunity; integrases; lifestyle; metagenomics; rapid methods; therapeutics; virulence
Abstract:
... The characterization of therapeutic phage genomes plays a crucial role in the success rate of phage therapies. There are three checkpoints that need to be examined for the selection of phage candidates, namely, the presence of temperate markers, antimicrobial resistance (AMR) genes, and virulence genes. However, currently, no single-step tools are available for this purpose. Hence, we have develop ...
DOI:
10.3390/v14020342

39. Photoelectrochemical biosensing platform based on in situ generated ultrathin covalent organic framework film and AgInS2 QDs for dual target detection of HIV and CEA

Author:
Xiaoshi Yan; Hongkun Li; Tengyue Yin; Guifen Jie; Hong Zhou
Source:
Biosensors & bioelectronics 2022 v.217 pp. 114694
ISSN:
0956-5663
Subject:
DNA; biosensors; electric current; nanogold; nucleic acid hybridization; oligonucleotides; rapid methods
Abstract:
... In this work, a new photoelectrochemical (PEC) biosensing platform based on an ordered two-dimensional (2D) ultrathin covalent organic framework (COF) film and AgInS₂ quantum dots (QDs) has been developed to enable dual-target detection of HIV and CEA. The porous COF film was firstly in situ generated on ITO, displaying super-stable and intense photocurrent with excellent repeatability. Moreover, ...
DOI:
10.1016/j.bios.2022.114694

40. Point-of-care diagnosis of invasive non-typhoidal Salmonella enterica in bloodstream infections using immunomagnetic capture and loop-mediated isothermal amplification

Author:
Aaydha C. Vinayaka; Mohsen Golabi; Thi Linh Quyen Than; Anders Wolff; Dang D. Bang
Source:
New biotechnology 2022 v.66 pp. 1-7
ISSN:
1871-6784
Subject:
DNA; Salmonella enterica; biotechnology; blood; blood flow; detection limit; diagnostic techniques; dyes; infrastructure; loop-mediated isothermal amplification; magnetism; malnutrition; pathogens; point-of-care testing; rapid methods; salmonellosis
Abstract:
... Invasive non-typhoidal salmonellosis is gaining worldwide attention as an emerging disease cluster among bloodstream infections. The disease has the highest burden among immunocompromised and malnourished children in resource-limited areas due to poor access to reliable and rapid diagnostics. Point-of-care (POC) diagnostics are promising for use in such low infrastructure laboratory settings. Howe ...
DOI:
10.1016/j.nbt.2021.08.003

41. RNA metabarcoding helps reveal zooplankton community response to environmental stressors

Author:
Phillip J. Ankley; Yuwei Xie; Sonya Havens; Lisa Peters; Lauren Timlick; Jose Luis Rodriguez-Gil; John P. Giesy; Vince P. Palace
Source:
Environmental pollution 2022 v.292 pp. 118446
ISSN:
0269-7491
Subject:
Arthropoda; DNA; DNA barcoding; RNA; Rotifera; anthropogenic activities; ecotoxicology; freshwater; oil spills; pollution; rapid methods; remediation; zooplankton
Abstract:
... DNA metabarcoding can provide a high-throughput and rapid method for characterizing responses of communities to environmental stressors. However, within bulk samples, DNA metabarcoding hardly distinguishes live from the dead organisms. Here, both DNA and RNA metabarcoding were applied and compared in experimental freshwater mesocosms conducted for assessment of ecotoxicological responses of zoopla ...
DOI:
10.1016/j.envpol.2021.118446

42. Rapid and sensitive detection of Karenia mikimotoi by loop-mediated isothermal amplification combined with a lateral flow dipstick

Author:
Xiaotian Han; Ting Zhao; Tian Yan; Rencheng Yu
Source:
Environmental science and pollution research 2022 v.29 no.17 pp. 24696-24703
ISSN:
0944-1344
Subject:
DNA; Karenia mikimotoi; biotin; computer software; hybridization; internal transcribed spacers; loop-mediated isothermal amplification; marine science; poisonous algae; pollution; rapid methods; research
Abstract:
... Harmful algal blooms frequently occur in various coastal regions worldwide, deteriorating marine ecology and causing huge economic losses. Therefore, developing a potential method for rapid detection of harmful algae species is highly necessitated. In this study, a loop-mediated isothermal amplification (LAMP) method coupled with a lateral flow dipstick (LFD) was developed for detecting the harmfu ...
DOI:
10.1007/s11356-021-17536-w

43. Rapid and ultrasensitive electrochemical detection of DNA methylation for ovarian cancer diagnosis

Author:
Dongfei Chen; Yanfang Wu; Richard D. Tilley; J. Justin Gooding
Source:
Biosensors & bioelectronics 2022 v.206 pp. 114126
ISSN:
0956-5663
Subject:
DNA; DNA methylation; biosensors; blood; cytosine; electrochemistry; epigenetics; magnetism; nucleotide sequences; ovarian neoplasms; rapid methods
Abstract:
... Alterations in DNA methylation, a stable epigenetic marker, are important components in the development of cancer. It is vital to develop diagnostic systems with the ability to rapidly quantify DNA methylation with high sensitivity and selectivity. However, the analysis of DNA methylation must address two main challenges: (i) ultralow abundance and (ii) differentiating methylated cytosine from nor ...
DOI:
10.1016/j.bios.2022.114126

44. Rapid detection of Chattonella marina by PCR combined with dot lateral flow strip

Author:
Chunyun Zhang; Qixin Chen; Fuguo Liu; Yin Liu; Yuanyuan Wang; Guofu Chen
Source:
Journal of applied phycology 2022 v.34 no.1 pp. 449-460
ISSN:
0921-8971
Subject:
Chattonella marina; DNA; algology; chromatography; cross reaction; detection limit; genes; microalgae; rapid methods
Abstract:
... In this study a novel technique referred to as PCR combined with dot lateral flow strip (PCDS) is proposed and its application to the detection of harmful microalgae was explored. For this purpose, using Chattonella marina as a test algal species, PCR targeting the D1–D2 region of large subunit ribosomal gene of this alga was performed with the tagged specific primers. The amplicons were then anal ...
DOI:
10.1007/s10811-021-02667-x

45. Rapid detection of Salmonella enterica in leafy greens by a novel DNA microarray-based PathogenDx system

Author:
Hsin-Bai Yin; Chi-Hung Chen; Benjamin Katchman; Cory Newland; Melissa May; Jitendra Patel
Source:
Food microbiology 2022 v.107 pp. 104086
ISSN:
0740-0020
Subject:
DNA; Lactuca sativa var. capitata; Salmonella Newport; food microbiology; fresh produce; inoculum; kale; microarray technology; rapid methods; romaine lettuce; spinach
Abstract:
... The diverse matrices pose great challenges for rapid detection of low Salmonella level (<10 CFU) in fresh produce. The applicability of microarray-based PathogenDx system for detecting low contamination of Salmonella Newport from leafy greens was evaluated. A pre-PCR preparation protocol including enrichment in universal pre-enrichment broth for 3 h followed by sample concentration using an Innova ...
DOI:
10.1016/j.fm.2022.104086
CHORUS:
10.1016/j.fm.2022.104086

46. Rapid screening of glycosyltransferases in plants using a linear DNA expression template based cell-free transcription-translation system

Author:
Shaobin Guo; Mingdi Wang; Wen Xu; Fuxian Zou; Jingjing Lin; Qin Peng; Wei Xu; Shaohua Xu; Xianai Shi
Source:
Phytochemistry 2022 v.193 pp. 113007
ISSN:
0031-9422
Subject:
Anoectochilus roxburghii; Arabidopsis thaliana; DNA; biosynthesis; catalytic activity; glycosylation; glycosyltransferases; isoquercitrin; medicinal plants; quercetin; rapid methods; transcriptome
Abstract:
... Plants have an extensively large number of enzymes including glycosyltransferases that are important in the biosynthesis of natural products. However, it is time-consuming and challenging to study these enzymes and only a small percentage of them have been well-characterized. Here, we report a rapid method to screen plant glycosyltransferases using a linear DNA expression template (LET) based cell ...
DOI:
10.1016/j.phytochem.2021.113007

47. Real-time Loop-Mediated Isothermal Amplification (LAMP) Using Self-quenching Fluorogenic Probes: the Application in Skipjack Tuna (Katsuwonus pelamis) Authentication

Author:
Wenjie Xu; Qiuping Li; Xiaowen Cui; Min Cao; Xiaohui Xiong; Libin Wang; Xiong Xiong
Source:
Food analytical methods 2022 v.15 no.3 pp. 658-665
ISSN:
1936-9751
Subject:
DNA; Katsuwonus pelamis; case studies; fluorescence; fluorescent dyes; loop-mediated isothermal amplification; rapid methods; tuna
Abstract:
... Target-specific detection of LAMP amplicons can be achieved through the use of target-specific probes or modified primers as biorecognition elements, decreasing the likelihood of detecting false-positive signals. The present work selected skipjack tuna as a case study, and developed a novel LAMP assay coupled with the self-quenching fluorogenic probe for rapid detection of skipjack tuna. Specifica ...
DOI:
10.1007/s12161-021-02159-1

48. Real-time recombinase-aided amplification with improved propidium monoazide for the rapid detection of viable Escherichia coli O157:H7 in milk

Author:
Dan Mu; Donggen Zhou; Guoyang Xie; Ju Liu; Zhengzheng Wang; Qin Xiong; Hengyi Xu
Source:
Journal of dairy science 2022 v.105 no.2 pp. 1028-1038
ISSN:
0022-0302
Subject:
DNA; Escherichia coli O157; chemical bonding; community health; dairy science; death; dyes; food matrix; hemolytic uremic syndrome; pathogenicity; propidium; rapid methods; skim milk; thrombocytopenic purpura
Abstract:
... Escherichia coli O157:H7, the causative agent of thrombotic thrombocytopenic purpura and hemolytic uremic syndrome in humans, generates a effective harm to community health because of its high pathogenicity. A real-time recombinase-aided amplification (rRAA) is an emerging method for nucleic acid detection. However, genomic DNA of bacteria could exist in food and the environment for a long time af ...
DOI:
10.3168/jds.2021-21074

49. SCORe: SARS-CoV-2 Omicron Variant RBD-Binding DNA Aptamer for Multiplexed Rapid Detection and Pseudovirus Neutralization

Author:
Lucy F. Yang; Nataly Kacherovsky; Joey Liang; Stephen J. Salipante; Suzie H. Pun
Source:
Analytical chemistry 2022 v.94 no.37 pp. 12683-12690
ISSN:
1520-6882
Subject:
COVID-19 infection; DNA; Pseudovirus; Severe acute respiratory syndrome coronavirus 2; analytical chemistry; diagnostic techniques; neutralization; oligonucleotides; pandemic; pathogenicity; rapid methods; viruses
Abstract:
... During the COVID-19 (coronavirus disease 2019) pandemic, several SARS-CoV-2 variants of concern emerged, including the Omicron variant, which has enhanced infectivity and immune invasion. Many antibodies and aptamers that bind the spike (S) of previous strains of SARS-CoV-2 either do not bind or bind with low affinity to Omicron S. In this study, we report a high-affinity SARS-CoV-2 Omicron RBD-bi ...
DOI:
10.1021/acs.analchem.2c01993

50. Simple direct DNA extraction protocols for efficient routine detection of plant pathogenic bacteria via conventional PCR

Author:
Miloud Sabri; El Hassan Achbani; Mohammed Diouri; Rachid Benkirane; Mohamed Ou-zine; Kaoutar El handi; Khaoula Habbadi
Source:
Journal of crop improvement 2022 v.36 no.4 pp. 514-525
ISSN:
1542-7536
Subject:
Agrobacterium radiobacter; DNA; Erwinia amylovora; Rhizobium vitis; apples; bovine serum albumin; chloroform; maceration; octoxynol; pears; phenol; polymerase chain reaction; polysorbates; polyvinylpyrrolidone; rapid methods; toxicity
Abstract:
... Rapid, easy and direct DNA extraction protocols are required for use in routine analyses to detect plant pathogenic bacteria. For this purpose, five protocols for extracting bacterial DNA, which do not use toxic chemicals such as phenol and chloroform, were optimized for the detection of plant pathogenic bacteria quickly and directly from plant material using conventional polymerase chain reaction ...
DOI:
10.1080/15427528.2021.1979158