U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject DNA Remove constraint Subject: DNA Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
Start Over

Search

68,046  Search Results

« Previous | 1 - 20 of 68,046 |
Number of results to display per page

Search Results

1. Ammonia-induced oxidative stress triggered proinflammatory response and apoptosis in pig lungs

Author:
Daojie Li; Long Shen; Di Zhang; Xiaotong Wang; Qiankun Wang; Wenhao Qin; Yun Gao; Xiaoping Li
Source:
Journal of environmental sciences (China) 2023 v.126 pp. 683-696
ISSN:
1001-0742
Subject:
DNA; Western blotting; ammonia; animal models; apoptosis; dose response; epithelium; genes; inflammation; lungs; metabolome; mineral fertilizers; oxidative phosphorylation; oxidative stress; protein synthesis; pyruvic acid; swine; toxicity; transcriptome; transmission electron microscopy; China
Abstract:
... Ammonia, a common toxic gas, is not only one of the main causes of haze, but also can enter respiratory tract and directly affect the health of humans and animals. Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung. In this study, the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using trans ...
DOI:
10.1016/j.jes.2022.05.005

2. Anchoring red blood cell with tetrahedral DNA nanostructure: Electrochemical biosensor for the sensitive signage of circulating tumor DNA

Author:
Xinyue Zha; Weiwei Qin; Jiadi Chen; Mengting Chen; Qiyue Zhang; Kaiyu He; Yingju Liu; Weipeng Liu
Source:
Talanta 2023 v.251 pp. 123793
ISSN:
0039-9140
Subject:
DNA; biocompatibility; biosensors; blood serum; detection limit; erythrocytes; methylene blue; neoplasms; rapid methods
Abstract:
... Circulating tumor cells (CTCs), as a type of tumor, have attracted wide attention because of their characteristics of shedding from the primary tumor and spreading to other tissues and organs through peripheral blood. The circulating tumor DNA (ctDNA), the DNA released by CTCs and other tumor cells into the peripheral blood, was considered as a promising detection substance for clinical applicatio ...
DOI:
10.1016/j.talanta.2022.123793

3. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement

Author:
Cuiying Lin; Qingqing Huang; Mengjian Tian; Fang Luo; Jian Wang; Bin Qiu; Shuofei Yang; Zhenyu Lin
Source:
Talanta 2023 v.251 pp. 123748
ISSN:
0039-9140
Subject:
DNA; adenine; biosensors; blood serum; detection limit; electrochemiluminescence; electrodes; ferrocenes; indium; methylation; methyltransferases; restriction endonucleases; tin
Abstract:
... In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the ...
DOI:
10.1016/j.talanta.2022.123748

4. Insights into a novel nitrogen removal process based on simultaneous anammox and denitrification (SAD) following nitritation with in-situ NOB elimination

Author:
Jing Chen; Jia Zeng; Yiran He; Shiquan Sun; Haipeng Wu; Yaoyu Zhou; Zhenguo Chen; Jianhui Wang; Hong Chen
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 160-170
ISSN:
1001-0742
Subject:
Comamonadaceae; DNA; anaerobic ammonium oxidation; batch systems; denitrification; nitrogen; total nitrogen; wastewater; wastewater treatment; zeolites; China
Abstract:
... Simultaneous anammox and denitrification (SAD) is an efficient approach to treat wastewater having a low C/N ratio; however, few studies have investigated a combination of SAD and partial nitritation (PN). In this study, a lab-scale up-flow blanket filter (UBF) and zeolite sequence batch reactor (ZSBR) were continuously operated to implement SAD and PN advantages, respectively. The UBF achieved a ...
DOI:
10.1016/j.jes.2022.01.019

5. An autonomous driven DNA walker-based electrochemical aptasensor for on-site detection of Ochratoxin A

Author:
Tongfu Huang; Mei Wang; Nian Hong; Hanfeng Cui; Qiqi Fan; Guobing Wei; Longshua Qin; Jing Zhang; Hao Fan
Source:
Talanta 2023 v.252 pp. 123785
ISSN:
0039-9140
Subject:
DNA; aptasensors; coordination polymers; electrochemistry; ochratoxin A
Abstract:
... Herein, an autonomous driven DNA walker-based aptasensor is proposed for the detection of Ochratoxin A (OTA). A nanoscale metal-organic framework (MOF) is synthesized and used to load Mn²⁺, a cofactor for DNAzyme. The DNA walker and Mn²⁺@MOF are assembled on the Au surface, integrating the DNA walker and metal auxiliary ions. The proposed sensor avoids the addition of metal auxiliary ions of DNAzy ...
DOI:
10.1016/j.talanta.2022.123785

6. A novel label-free universal biosensing platform based on CRISPR/Cas12a for biomarker detection

Author:
Xiaomei Mu; Xin Wang; Yuxin Qin; Yong Huang; Jianniao Tian; Shulin Zhao
Source:
Talanta 2023 v.251 pp. 123795
ISSN:
0039-9140
Subject:
DNA; biomarkers; biosensors; disease surveillance; enzyme activity; fluorescence; hydrolysis; microRNA; nanosilver
Abstract:
... The development of a biosensing platform with high sensitivity, high specificity, and low cost for the detection of biomarkers, especially one that is programmable and universal, is critical for disease surveillance and diagnosis, yet it remains a difficulty. Herein, we combined the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system with a fluorescent label-free biosenso ...
DOI:
10.1016/j.talanta.2022.123795

7. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

8. 100 Years of Chromosome Research in Rye, Secale L.

Author:
Rolf Schlegel
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
DNA; barley; cytogenetic analysis; fish; fluorescence; genes; grasses; haploidy; meiosis; microscopy; mitosis; reciprocal translocation; rice; rye; sequence analysis; tetraploidy; trisomics; wheat
Abstract:
... Although microscopy and genetics were still in their infancy, there are cytological results produced a hundred years ago that are still relevant today. Since the 1920s, rye has been a subject of chromosome research. It started by plotting its mitotic and meiotic chromosomes to determine genome size. After controversial evidence, it became clear that the base number is n = 7. However, structural di ...
DOI:
10.3390/plants11131753

9. 19th century family saga re-told by DNA recovered from postcard stamps

Author:
Cordula Haas; Christian Körner; Andrea Sulzer; Adelgunde Kratzer
Source:
Forensic science international 2022 v.330 pp. 111129
ISSN:
0379-0738
Subject:
DNA; children; forensic sciences; males; saliva
Abstract:
... Old postcards with stamps might help unravelling historical family stories and relationships. By employing ancient DNA recovered from world war I postage stamps, we disprove a family saga of an illegitimate child born in 1887. We developed a protocol to collect DNA from saliva, trapped and protected on the backside of postage stamps glued on postcards. With replicate STR analyses we were able to a ...
DOI:
10.1016/j.forsciint.2021.111129

10. 2,7-Diazapyrenes: a brief review on synthetic strategies and application opportunities

Author:
Anindita Mukherjee; Alexey A. Akulov; Sougata Santra; Mikhail V. Varaksin; Grigory A. Kim; Dmitry S. Kopchuk; Olga S. Taniya; Grigory V. Zyryanov; Oleg. N. Chupakhin
Source:
RSC advances 2022 v.12 no.15 pp. 9323-9341
ISSN:
2046-2069
Subject:
DNA; electronics; geometry; moieties
Abstract:
... 2,7-Diazapyrenes are promising azaaromatic scaffolds with a unique structural geometry and supramolecular properties. This core moiety and its derivatives with some N-methyl cations like N-methyl-2,7,-diazapyrenium, and N,N′-dimethyl-2,7-diazapyrenium attract special attention due to their challenging photophysical properties, especially in the context of interactions with DNA and some of its mono ...
DOI:
10.1039/d2ra00260d

11. 2-Oxopurine Riboside: A Dual Fluorescent Analog and Photosensitizer for RNA/DNA Research

Author:
Naishka E. Caldero-Rodríguez; Enrique M. Arpa; Diego J. Cárdenas; Lara Martínez-Fernández; Steffen Jockusch; Sourav Kanti Seth; Inés Corral; Carlos E. Crespo-Hernández
Source:
Journal of physical chemistry 2022 v.126 no.24 pp. 4483-4490
ISSN:
1520-5207
Subject:
DNA; RNA; absorption; aqueous solutions; fluorescence; nucleosides; photolysis; photosensitizing agents; quantum mechanics; singlet oxygen; solvents
Abstract:
... There is significant interest in developing suitable nucleoside analogs exhibiting high fluorescence and triplet yields to investigate the structure, dynamics, and binding properties of nucleic acids and promote selective photosensitized damage to DNA/RNA, respectively. In this study, steady-state, laser flash photolysis, time-resolved IR luminescence, and femtosecond broad-band transient absorpti ...
DOI:
10.1021/acs.jpcb.2c01113

12. 2D DNA lattices assembled from DX-coupled tiles

Author:
Wei Zhang; Chuan Jiang; Xin Guo; Mirza Muhammad Faran Ashraf Baig; Caihong Ni; Shou-Jun Xiao
Source:
Journal of colloid and interface science 2022 v.616 pp. 499-508
ISSN:
0021-9797
Subject:
DNA; atomic force microscopy; mechanics; mica; oligonucleotides
Abstract:
... Formation of coupled double crossovers (DXs) in a circular 106-mer oligonucleotide (c106nt) could generate stable tiles with the tile core span of 10 half turns. The large-span tiles with complicated curvatures and mechanics could assemble 2D lattices under different environments. Hence, 2D DNA lattice structures based on tile types and sequences, tile packing modes of corrugation and non-corrugat ...
DOI:
10.1016/j.jcis.2022.02.038

13. 2D morphometric analysis of Arabidopsis thaliana nuclei reveals characteristic profiles of different cell types and accessions

Author:
Penka Pavlova; Martijn van Zanten; Basten L. Snoek; Hans de Jong; Paul Fransz
Source:
Chromosome research 2022 v.30 no.1 pp. 5-24
ISSN:
0967-3849
Subject:
Arabidopsis thaliana; DNA; chromocenters; fluorescence microscopy; heterochromatin; morphometry; phenotype; research; transcription (genetics); transposons
Abstract:
... Functional changes of cells upon developmental switches and in response to environmental cues are often reflected in nuclear phenotypes, showing distinctive chromatin states corresponding to transcriptional changes. Such characteristic nuclear shapes have been microscopically monitored and can be quantified after differential staining of euchromatin and heterochromatin domains. Here, we examined s ...
DOI:
10.1007/s10577-021-09673-2

14. 2q35-rs13387042 variant and the risk of breast cancer: a case–control study

Author:
Abolfazl Nesaei; Zari Naderi Ghale-noie; Asma Khorshid Shamshiri; Fahimeh Afzaljavan; Mahdi Rivandi; Amir Tajbakhsh; Fatemeh Homaei Shandiz; Alireza Pasdar
Source:
Molecular biology reports 2022 v.49 no.5 pp. 3549-3557
ISSN:
0301-4851
Subject:
DNA; alleles; blood; breast neoplasms; breasts; carcinogenesis; case-control studies; demographic statistics; etiology; genetic background; genotyping; histopathology; lifestyle; molecular biology; risk; risk assessment; statistical analysis
Abstract:
... BACKGROUND: Breast Cancer is the most frequent neoplasm diagnosed among women worldwide. Genetic background and lifestyle/environment play a significant role in the disease etiology. According to Genome-wide association studies, some single-nucleotide polymorphisms such as 2q35-rs13387042–(G/A) have been introduced to be associated with breast cancer risk and features. In this study, we aimed to e ...
DOI:
10.1007/s11033-022-07195-6

15. 3, 4-Dihydroxy-l-phenylalanine Biopolymer Cellulose DNA Adhesive Card as an Enhanced Solid-Phase One-Step DNA Extraction Method from Foodborne Pathogens in Food Samples

Author:
Carole D. V. Nanfack; Jia Yang; Xiao Yuan; Jiadi Sun; Xiulan Sun; Jian Ji
Source:
Food analytical methods 2022 v.15 no.8 pp. 2069-2083
ISSN:
1936-9751
Subject:
3,4-dihydroxyphenylalanine oxidative deaminase; DNA; Fourier transform infrared spectroscopy; L-dopa; X-ray diffraction; biopolymers; cellulose; chickens; food pathogens; infrastructure; microbial detection
Abstract:
... Deoxyribonucleic acid (DNA) extraction, which is the mainstay of molecular amplification for food pathogen detection procedure, is dependent on laboratory infrastructure, cumbersome methods, and time consumption. To tackle these dependencies, we developed an enhanced mussel-inspired cellulose card for the convenient, eco-friendly, and rapid DNA extraction from bacteria present in food. This enhanc ...
DOI:
10.1007/s12161-021-02177-z

16. A 638‐gene phylogeny supports the recognition of twice as many species in the Malagasy endemic genus Capurodendron (Sapotaceae)

Author:
Carlos G. Boluda; Camille Christe; Yamama Naciri; Laurent Gautier
Source:
Taxon 2022 v.71 no.2 pp. 360-395
ISSN:
0040-0262
Subject:
DNA; Sapotaceae; conservation status; extinction; hardwood; new species; phylogeny; risk; statistical analysis; Madagascar
Abstract:
... The Malagasy genus Capurodendron currently accommodates 26 described species and is the largest genus of the family Sapotaceae in Madagascar. These species are frequently logged because of their valued hardwood, which potentially puts them at risk of extinction. Species‐level identifications are often problematic, and this hinders both an accurate assessment of their conservation status and the de ...
DOI:
10.1002/tax.12676

17. An 82 bp tandem repeat family typical of 3′ non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres

Author:
Leonardo Adabo Cintra; Thaíssa Boldieri de Souza; Letícia Maria Parteka; Lucas Mesquita Barreto; Luiz Filipe Protasio Pereira; Marcos Letaif Gaeta; Romain Guyot; André Luís Laforga Vanzela
Source:
Genome 2022 v.65 no.3 pp. 137-151
ISSN:
1480-3321
Subject:
DNA; allotetraploidy; centromeres; chromatin; genome; microsatellite repeats; non-coding RNA; retrotransposons
Abstract:
... Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and ...
DOI:
10.1139/gen-2021-0045

18. 90-day nose-only inhalation toxicity study of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in Sprague-Dawley rats

Author:
Shu-Chieh Hu; Seonggi Min; Hyun-Ki Kang; Dong-Jin Yang; Mallikarjuna Basavarajappa; Sherry M. Lewis; Kelly J. Davis; Ralph E. Patton; Matthew S. Bryant; Estatira Sepehr; Raul Trbojevich; Mason G. Pearce; Michelle E. Bishop; Wei Ding; Robert H. Heflich; MacKean P. Maisha; Robert Felton; Susan Chemerynski; Steven B. Yee; Melis Coraggio; Hans Rosenfeldt; R. Philip Yeager; Paul C. Howard; Yunan Tang
Source:
Food and chemical toxicology 2022 v.160 pp. 112780
ISSN:
0278-6915
Subject:
DNA; aerosols; air; biomarkers; blood; body weight; bone marrow; breathing; carcinogenesis; carcinogenicity; cigarettes; comet assay; food consumption; genotoxicity; histopathology; humans; inhalation toxicity; lowest observed effect level; lungs; metabolites; mutagens; nitrosamines; no observed adverse effect level; nose; propylene glycol; urine
Abstract:
... 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is one of the key tobacco-specific nitrosamines that plays an important role in human lung carcinogenesis. Repeated dose inhalation toxicity data on NNK, particularly relevant to cigarette smoking, however, is surprisingly limited. Hence, there is a lack of direct information available on the carcinogenic and potential non-carcinogenic effects o ...
DOI:
10.1016/j.fct.2021.112780

19. The ACTN3 R577X polymorphism is associated with metabolic alterations in a sex‐dependent manner in subjects from western Mexico

Author:
Elisa Barrón‐Cabrera; Nathaly Torres‐Castillo; Karina González‐Becerra; Eloy A. Zepeda‐Carrillo; Rafael Torres‐Valadez; Iván Hernández‐Cañaveral; Erika Martínez‐López
Source:
Journal of human nutrition and dietetics 2022 v.35 no.4 pp. 713-721
ISSN:
0952-3871
Subject:
DNA; bioelectrical impedance; body composition; chemistry; computer software; cross-sectional studies; dietetics; genes; genotype; genotyping; glucose; hypertriglyceridemia; insulin resistance; lifestyle; metabolism; muscles; skeletal muscle; triacylglycerols; Mexico
Abstract:
... BACKGROUND: The ACTN3 gene is primarily expressed in fast skeletal muscle fibres. A common nonsense polymorphism in this gene is ACTN3 R577X (rs1815739), which causes an absolute deficiency of α‐actinin‐3 protein and alterations in muscle metabolism. Considering metabolic alterations are influenced by nutrition and genetic factors, as well as lifestyle factors, we hypothesise a possible associatio ...
DOI:
10.1111/jhn.12948

20. AGAMEMNON: an Accurate metaGenomics And MEtatranscriptoMics quaNtificatiON analysis suite

Author:
Giorgos Skoufos; Fatemeh Almodaresi; Mohsen Zakeri; Joseph N. Paulson; Rob Patro; Artemis G. Hatzigeorgiou; Ioannis S. Vlachos
Source:
Genome biology 2022 v.23 no.1 pp. 39
ISSN:
1474-760X
Subject:
DNA; RNA; data collection; genome; host specificity; metagenomics; transcriptomics
Abstract:
... We introduce AGAMEMNON (https://github.com/ivlachos/agamemnon) for the acquisition of microbial abundances from shotgun metagenomics and metatranscriptomic samples, single-microbe sequencing experiments, or sequenced host samples. AGAMEMNON delivers accurate abundances at genus, species, and strain resolution. It incorporates a time and space-efficient indexing scheme for fast pattern matching, en ...
DOI:
10.1186/s13059-022-02610-4