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You searched for: Subject DNA primers Remove constraint Subject: DNA primers Text Availability Full Text Remove constraint Text Availability: Full Text
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1. A DNA sequencing strategy that requires only five bases of known terminal sequence for priming

Author:
Fu, D.J.; Broude, N.E.; Koster, H.; Smith, C.L.; Cantor, C.R.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.22 pp. 10162-10166
ISSN:
0027-8424
Subject:
sequence analysis; DNA; DNA primers
Abstract:
... We have previously reported an enhanced version of sequencing by hybridization (SBH), termed positional SBH (PSBH). PSBH uses partially duplex probes containing single-stranded 3' overhangs, instead of simple single-stranded probes. Stacking interactions between the duplex probe and a single-stranded target allow us to reduce the probe sizes required to 5-base single-stranded overhangs. Here we de ...
DOI:
10.1073/pnas.92.22.10162
PubMed:
7479746
PubMed Central:
PMC40756

2. Fluorescences energy transfer dye-labeled primers for DNA sequencing and analysis

Author:
Ju, J.Y.; Ruan, C.C.; Fuller, C.W.; Glazer, A.N.; Mathies, R.A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.10 pp. 4347-4351
ISSN:
0027-8424
Subject:
sequence analysis; DNA primers; DNA; electron transfer; fluorescein
Abstract:
... Fluorescent dye-labeled DNA primers have been developed that exploit fluorescence energy transfer (ET) to optimize the absorption and emission properties of the label. These primers carry a fluorescein derivative at the 5' end as a common donor and other fluorescein and rhodamine derivatives attached to a modified thymidine residue within the primer sequence as acceptors. Adjustment of the donor-a ...
DOI:
10.1073/pnas.92.10.4347
PubMed:
7753809
PubMed Central:
PMC41941

3. Isolation of a superfamily of candidate disease-resistance genes in soybean based on a conserved nucleotide-binding site

Author:
Yu, Y.G.; Buss, G.R.; Saghai Maroof, M.A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1996 v.93 no.21 pp. 11751-11756
ISSN:
0027-8424
Subject:
restriction fragment length polymorphism; Arabidopsis thaliana; plant diseases and disorders; Glycine max; genetic resistance; Linum usitatissimum; DNA; linkage groups; DNA primers; binding proteins; chromosome mapping; Nicotiana tabacum; nucleotide sequences; binding sites; multigene family; genetic markers; plant proteins
Abstract:
... The tobacco N and Arabidopsis RPS2 genes, among several recently cloned disease-resistance genes, share a highly conserved structure, a nucleotide-binding site (NBS). Using degenerate oligonucleotide primers for the NBS region of N and RPS2, we have amplified and cloned the NBS sequences from soybean. Each of these PCR-derived NBS clones detected low- or moderate-copy soybean DNA sequences and bel ...
DOI:
10.1073/pnas.93.21.11751
PubMed:
8876209
PubMed Central:
PMC38130

4. Analysis of hybrids of durum wheat with Thinopyrum junceiforme using RAPD markers

Author:
Bommineni, V.R.; Jauhar, P.P.; Peterson, T.S.; Chibbar, R.N.; Almouslem, A.B.
Source:
Theoretical and applied genetics 1997 v.95 no.5/6 pp. 757-763
ISSN:
0040-5752
Subject:
Triticum turgidum; Southern blotting; Elytrigia; cultivars; hybrids; restriction fragment length polymorphism; intergeneric hybridization; random amplified polymorphic DNA technique; backcrossing; Thinopyrum pycnanthum; DNA primers; Leymus; DNA; genomics; Elymus; Thinopyrum elongatum; Thinopyrum bessarabicum; genetic markers
Abstract:
... The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n = 4x = 28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n = 4x = 28; J1J1J2J2) using RAPD markers. The first step was to identify amplification of species-specific DNA markers in the parental grass species and durum wheat cultivars. Initially, t ...
Handle:
10113/16398
DOI:
10.1007/s001220050622

5. Screening for overlapping bacterial artificial chromosome clones by PCR analysis with an arbitrary primer

Author:
Xu, J.C.; Yang, D.C.; Domingo, J.; Ni, J.J.; Huang, N.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1998 v.95 no.10 pp. 5661-5666
ISSN:
0027-8424
Subject:
Oryza sativa; DNA libraries; clones; screening; polymerase chain reaction; DNA; loci; nucleotide sequences; DNA fingerprinting; genome; DNA primers
Abstract:
... In this article, we used PCR analysis with arbitrary primers (AP-PCR) to screen for overlapping bacterial artificial chromosome (BAC) clones and assembly of contigs. A rice BAC library with three genome equivalents was used to prepare pooled BAC DNA. Twenty-two arbitrary primers were used to survey the pooled BAC DNAs and individual BAC DNAs. Each primer identified 1-10 loci, and the average was 4 ...
DOI:
10.1073/pnas.95.10.5661
PubMed:
9576940
PubMed Central:
PMC20435

6. The potential for cross-taxa simple-sequence repeat (SSR) amplification between Arabidopsis thaliana L. and crop brassicas

Author:
Westman, A.L.; Kresovich, S.
Source:
Theoretical and applied genetics 1998 v.96 no.2 pp. 272-281
ISSN:
0040-5752
Subject:
Arabidopsis thaliana; nucleic acid hybridization; interspecific variation; microsatellite repeats; Brassica nigra; DNA primers; DNA; Brassica rapa subsp. oleifera; polymerase chain reaction; Brassica juncea; Brassica napus; Brassica oleracea; genetic variation; Brassica carinata
Abstract:
... Simple-sequence repeats (SSRs) may prove to be valuable descriptors of plant genetic variation, but developing markers for each of many taxa is costly. The applicability of SSR markers may be increased if they can be amplified across taxa. In this study, we examined whether 30 primer pairs designed to amplify single-locus SSRs in Arabidopsis thaliana could be used to amplify marker loci in six Bra ...
Handle:
10113/14333
DOI:
10.1007/s001220050736

7. Detection of multiresistant Salmonella typhimurium DT104 using multiplex and fluorogenic PCR

Author:
Carlson, S.A.; Bolton, L.F.; Briggs, C.E.; Hurd, H.S.; Sharma, V.K.; Fedorka-Cray, P.J.; Jones, B.D.
Source:
MOLECULAR AND CELLULAR PROBES 1999 v.13 no.3 pp. 213-222
ISSN:
1096-1194
Subject:
chloramphenicol; diagnostic techniques; disease detection; sulfamethoxazole; tetracycline; phenotype; antibiotic resistance; DNA primers; DNA; Salmonella Typhimurium; multiple drug resistance; polymerase chain reaction; diagnostic specificity; streptomycin; salmonellosis; genes; ampicillin; digestive system diseases; pathogens
Abstract:
... Salmonella infections continue to cause gastrointestinal and systemic disease throughout the world. Salmonella typhimurium DT104 further poses a major health concern due to its acquisition of resistance to multiple antibiotics. The rapid detection of multiresistant S. typhimurium DT104 would facilitate strategies aimed at controlling this pathogen. We developed a specific and sensitive polymerase ...
Handle:
10113/53830
DOI:
10.1006/mcpr.1999.0240
PubMed:
10369747

8. Octamer-based genome scanning distinguishes a unique subpopulation of Escherichia coli O157:H7 strains in cattle

Author:
Kim, J.; Nietfeldt, J.; Benson, A.K.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.23 pp. 13288-13293
ISSN:
0027-8424
Subject:
restriction fragment length polymorphism; geographical distribution; humans; DNA primers; DNA; phylogeny; bacteriophages; dairy cattle; proviruses; genotype; nucleotide sequences; population; bacteria; Escherichia coli
Abstract:
... Multilocus-genotyping methods have shown that Escherichia coli O157:H7 is a geographically disseminated clone. However, high-resolution methods such as pulse-field gel electrophoresis demonstrate significant genomic diversity among different isolates. To assess the genetic relationship of human and bovine isolates of E. coli O157:H7 in detail, we have developed an octamer-based genome-scanning met ...
DOI:
10.1073/pnas.96.23.13288
PubMed:
10557313
PubMed Central:
PMC23940

9. Phylogenetic diversity of methanogenic archaea in swine waste storage pits

Author:
Whitehead, T.R.; Cotta, M.A.
Source:
FEMS microbiology letters 1999 v.179 no.2 pp. 223-226
ISSN:
0378-1097
Subject:
pig manure; feces; manure storage; Archaea; polymerase chain reaction; DNA primers; ribosomal DNA; nucleotide sequences
Abstract:
... Total DNA was isolated from swine feces and a swine waste storage pit and used as templates for PCR amplification of archaeal 16S rDNA using specific primers. Only the sample from the center of the waste pit produced a PCR product. DNA sequence analyses of random clones demonstrated a variety of methanogenic archaea. Six groups of sequences were identified, including those similar to Methanobrevib ...
Handle:
10113/26191
PubMed:
10518719

10. Semi-automated fluorogenic PCR assays (TaqMan) for rapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coli

Author:
Sharma, V.K.; Dean-Nystrom, E.A.; Casey, T.A.
Source:
Molecular and cellular probes 1999 v.13 no.4 pp. 291-302
ISSN:
1096-1194
Subject:
bacterial contamination; DNA primers; food contamination; polymerase chain reaction; automation; feces; food microbiology; genes; microbial detection; beef; Escherichia coli O157; fluorescent dyes
Abstract:
... Semi-automated detection of Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 and non-O157:H7 Shiga toxin-producing E. coli (STEC) was achieved using fluorogenic polymerase chain reaction (PCR). These PCR assays were designed to amplify 80, 120 and 150 bp regions of virulence genes stx1 , stx2 and eaeA , respectively, using specific primers. The fluorogenic probes were used for specific detection ...
Handle:
10113/7998
DOI:
10.1006/mcpr.1999.0251
PubMed:
10441202

11. Application of the 5'-nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni

Author:
Nogva, H.K.; Bergh, A.; Holck, A.; Rudi, K.
Source:
Applied and environmental microbiology 2000 v.66 no.9 pp. 4029-4036
ISSN:
0099-2240
Subject:
polymerase chain reaction; nucleases; food microbiology; DNA primers
Abstract:
... Campylobacter jejuni is recognized as a leading human food-borne pathogen. Traditional diagnostic testing for C. jejuni is not reliable due to special growth requirements and the possibility that this bacterium can enter a viable but nonculturable state. Nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. In this article, we present a 5'-nuclease PCR as ...
DOI:
10.1128/AEM.66.9.4029-4036.2000
PubMed:
10966425
PubMed Central:
PMC92255

12. Characterization of an Unidentified Sarcocystis falcatula-like Parasite from the South American Opossum, Didelphis albiventris from Brazil

Author:
DUBEY J. P.; LINDSAY D. S.; REZENDE P. C. B.; COSTA A. J.
Source:
journal of eukaryotic microbiology 2000 v.47 no.6 pp. 538-544
ISSN:
1066-5234
Subject:
Sarcocystis falcatula; sarcocysts; budgerigars; lungs; Didelphis albiventris; opossums; intestines; mortality; sarcocystosis; merozoites; schizonts; protozoal infections; cell culture; polymerase chain reaction; DNA primers; disease detection; Brazil
Abstract:
... An unidentified isolate of a Sarcocystis falcatula-like parasite was obtained from the lungs of budgerigars (Melopsittacus undulatus) fed sporocysts from a naturally-infected South American opossum, Didelphis albiventris from Brazil. Four captive budgerigars fed sporocysts from the opossum intestine died of acute sarcocystosis 8, 10, and 12 days after oral inoculation (DAI); one budgerigar was kil ...
Handle:
10113/49360
DOI:
10.1111/j.1550-7408.2000.tb00087.x

13. Control of fruit ripening through genetic control of ACC synthase synthesis

Author:
Theologis, A.; Sato, T.
Source:
United States Department of Agriculture patents 2000 no.6,156,956 pp. -
Subject:
tomatoes; patents; Cucumis sativus; DNA primers; cucumbers; polymerase chain reaction; amino acid sequences; gene expression; multigene family; ripening; nucleotide sequences; 1-aminocyclopropane-1-carboxylate synthase; USDA; enzyme activity; Solanum lycopersicum var. lycopersicum; United States
Abstract:
... ACC synthases of higher plants are coded by multigene families; only certain members of these families are responsible for various plant development characteristics effected by ethylene. Control of the processes in plants which are mediated by ACC synthase, such as fruit ripening, can be effected by controlling expression of the relevant ACC synthase gene. In addition, comparison of the amino acid ...
Handle:
10113/6333

14. Detection and identification of bacterial endosymbionts in arbuscular mycorrhizal fungi belonging to the family Gigasporaceae

Author:
Bianciotto, V.; Lumini, E.; Lanfranco, L.; Minerdi, D.; Bonfante, P.; Perotto, S.
Source:
Applied and environmental microbiology 2000 v.66 no.10 pp. 4503-4509
ISSN:
0099-2240
Subject:
symbionts; Gigaspora margarita; polymerase chain reaction; ribosomal DNA; fluorescence; phylogeny; DNA primers; nucleotide sequences; fungal spores; nucleic acid hybridization; bacteria; Endogonales
Abstract:
... Intracellular bacteria have been found previously in one isolate of the arbuscular mycorrhizal (AM) fungus Gigaspora margarita BEG 34. In this study, we extended our investigation to 11 fungal isolates obtained from different geographic areas and belonging to six different species of the family Gigasporaceae. With the exception of Gigaspora rosea, isolates of all of the AM species harbored bacteri ...
DOI:
10.1128/AEM.66.10.4503-4509.2000
PubMed:
11010905
PubMed Central:
PMC92331

15. Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA

Author:
Jasalavich, C.A.; Ostrofsky, A.; Jellison, J.
Source:
Applied and environmental microbiology 2000 v.66 no.11 pp. 4725-4734
ISSN:
0099-2240
Subject:
restriction fragment length polymorphism; ribosomal RNA; ribosomal DNA; Ascomycota; polymerase chain reaction; decayed wood; DNA primers; brown-rot fungi; internal transcribed spacers; white-rot fungi; genes; Basidiomycota; Picea; sapwood
DOI:
10.1128/AEM.66.11.4725-4734.2000
PubMed:
11055916
PubMed Central:
PMC92372

16. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-species PCR primers

Author:
Walter, J.; Tannock, G.W.; Tilsala-Timisjarvi, A.; Rodtong, S.; Loach, D.M.; Munro, K.; Alatossava, T.
Source:
Applied and environmental microbiology 2000 v.66 no.1 pp. 297-303
ISSN:
0099-2240
Subject:
DNA fragmentation; DNA primers; Lactobacillus; denaturing gradient gel electrophoresis; genes; genetic databases; humans; intergenic DNA; mice; nucleotide sequences; polymerase chain reaction; ribosomal RNA; stomach; swine
Abstract:
... Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR pr ...
DOI:
10.1128/AEM.66.1.297-303.2000
PubMed:
10618239
PubMed Central:
PMC91821

17. Development of multiplex sets of simple sequence repeat DNA markers covering the soybean genome

Author:
Narvel, J.M.; Chu, W.C.; Fehr, W.R.; Cregan, P.B.; Shoemaker, R.C.
Source:
Molecular breeding 2000 v.6 no.2 pp. 175-183
ISSN:
1380-3743
Subject:
microsatellite repeats; Glycine max; alleles; loci; linkage groups; DNA; polymerase chain reaction; DNA primers; genetic markers
Abstract:
... Multiplexing involves the analysis of several markers in a single gel lane that is based on the allele size range of marker loci. Multiplex SSR marker analysis is conducted with primers that are labeled with one of three dyes. The development of an SSR multiplex system requires estimates of the allele size range of markers to strategize primer labeling and for grouping markers into multiplex sets. ...
Handle:
10113/30848

18. Genetic characterization of soybean rhizobia in Paraguay

Author:
Chen, L.S.; Figueredo, A.; Pedrosa, F.O.; Hungria, M.
Source:
Applied and environmental microbiology 2000 v.66 no.11 pp. 5099-5103
ISSN:
0099-2240
Subject:
carbohydrate metabolism; Glycine max; ribosomal DNA; polymerase chain reaction; DNA primers; root nodules; Bradyrhizobium elkanii; repetitive sequences; Sinorhizobium fredii; Bradyrhizobium japonicum; mannitol; genetic variation; Paraguay
DOI:
10.1128/AEM.66.11.5099-5103.2000
PubMed:
11055970
PubMed Central:
PMC92426

19. Identification of a universally primed-PCR-derived sequence-characterized amplified region marker for an antagonistic strain of Clonostachys rosea and development of a strain-specific PCR detection assay

Author:
Bulat, S.A.; Lubeck, M.; Alekhina, I.A.; Jensen, D.F.; Knudsen, I.M.B.; Lubeck, P.S.
Source:
Applied and environmental microbiology 2000 v.66 no.11 pp. 4758-4763
ISSN:
0099-2240
Subject:
fungal antagonists; agricultural soils; Deuteromycotina; polymerase chain reaction; DNA; introduced species; DNA primers; Clonostachys rosea; nucleotide sequences; genetic markers; Denmark
DOI:
10.1128/AEM.66.11.4758-4763.2000
PubMed:
11055920
PubMed Central:
PMC92376

20. The MITE family Heartbreaker (Hbr): molecular markers in maize

Author:
Casa, A.M.; Brouwer, C.; Nagel, A.; Wang, L.; Zhang, Q.; Kresovich, S.; Wessler, S.R.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2000 v.97 no.18 pp. 10083-10089
ISSN:
0027-8424
Subject:
restriction fragment length polymorphism; Zea mays; amplified fragment length polymorphism; DNA primers; DNA; chromosome mapping; segregation distortion; transposons; genetic markers
DOI:
10.1073/pnas.97.18.10083
PubMed:
10963671
PubMed Central:
PMC27704