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You searched for: Subject DNA-directed RNA polymerase Remove constraint Subject: DNA-directed RNA polymerase Subject gene expression Remove constraint Subject: gene expression Subject DNA microarrays Remove constraint Subject: DNA microarrays
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1. Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia

Author:
Masuo, Shunsuke; Terabayashi, Yasunobu; Shimizu, Motoyuki; Fujii, Tatsuya; Kitazume, Tatsuya; Takaya, Naoki
Source:
Molecular genetics and genomics 2010 v.284 no.6 pp. 415-424
ISSN:
1617-4615
Subject:
Aspergillus nidulans; DNA microarrays; DNA-directed RNA polymerase; NAD (coenzyme); aeration; anaerobic conditions; ethanol; ethanol production; fungi; gamma-aminobutyric acid; gene expression; gene expression regulation; genes; glucose; glycolysis; hypoxia; messenger RNA; normoxia; oxygen; transcription (genetics); tricarboxylic acid cycle
Abstract:
... Hypoxia imposes a challenge upon most filamentous fungi that require oxygen for proliferation. Here, we used whole genome DNA microarrays to investigate global transcriptional changes in Aspergillus nidulans gene expression after exposure to hypoxia followed by normoxia. Aeration affected the expression of 2,864 genes (27% of the total number of genes in the fungus), of which 50% were either induc ...
DOI:
10.1007/s00438-010-0576-x

2. Disruption of essential plastid gene expression caused by T7 RNA polymerase-mediated transcription of plastid transgenes during early seedling development

Author:
Magee, Alan M.; MacLean, Daniel; Gray, John C.; Kavanagh, Tony A.
Source:
Transgenic research 2007 v.16 no.4 pp. 415-428
ISSN:
0962-8819
Subject:
DNA microarrays; DNA-directed RNA polymerase; bacteriophages; biotechnology; gels; gene expression; gene expression regulation; genetically modified organisms; leaves; mutants; phenotype; proteins; ribosomal RNA; seedlings; tobacco; transcription (genetics); transgenes
Abstract:
... Transcription of plastid transgenes by plastid-targeted T7 RNA polymerase (ptT7RNAP) during early seedling development in tobacco was associated with a pale-green leaf phenotype, depletion of plastid rRNAs and arrest of shoot development. Extensive analysis of mutant seedlings at the transcript level using DNA microarrays and RNA gel blotting revealed severe disruption of plastid rRNA accumulation ...
DOI:
10.1007/s11248-006-9045-z
PubMed:
17103239

3. The role of the omega subunit of RNA polymerase in expression of the relA gene in Escherichia coli

Author:
Chatterji, Dipankar; Ogawa, Yoshito; Shimada, Tomohiro; Ishihama, Akira
Source:
FEMS microbiology letters 2007 v.267 no.1 pp. 51-55
ISSN:
0378-1097
Subject:
messenger RNA; starvation; stress response; DNA microarrays; gene expression; ribosomal proteins; operon; DNA-directed RNA polymerase; Escherichia coli
Abstract:
... The rpoZ gene for the omega subunit of Escherichia coli RNA polymerase constitutes single operon with the spoT gene, which is responsible for the maintenance of stringent response under nutrient starvation conditions. To identify the physiological role of the omega subunit, we compared the gene expression profile of wild-type Escherichia coli with that of an rpoZ deleted strain by microarray analy ...
DOI:
10.1111/j.1574-6968.2006.00532.x

4. DNA Microarray Analysis of Plastid Gene Expression in an Arabidopsis Mutant Deficient in a Plastid Transcription Factor Sigma, SIG2

Author:
Akitomo NAGASHIMA; Mitsumasa HANAOKA; Reiko MOTOHASHI; Motoaki SEKI; Kazuo SHINOZAKI; Kengo KANAMARU; Hideo TAKAHASHI; Kan TANAKA
Source:
Bioscience, biotechnology, and biochemistry 2004 v.68 no.3 pp. 694-704
ISSN:
1347-6947
Subject:
Arabidopsis thaliana; DNA microarrays; DNA-directed RNA polymerase; biotechnology; chloroplast proteins; chloroplasts; gene expression; mutants; nuclear genome; photosynthesis; plastid genome
Abstract:
... The plastid genome of higher plants contains more than one hundred genes for photosynthesis, gene expression, and other processes. Plastid transcription is done by two types of RNA polymerase, PEP and NEP. PEP is a eubacteria-type RNA polymerase that is essential for chloroplast development. In Arabidopsis thaliana, six sigma factors (SIG1-6) are encoded by the nuclear genome, and postulated to de ...
DOI:
10.1271/bbb.68.694