U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject Danio rerio Remove constraint Subject: Danio rerio Text Availability Citation in PubAg Remove constraint Text Availability: Citation in PubAg
Start Over

Search

8,484  Search Results

« Previous | 1 - 100 of 8,484 |
Number of results to display per page

Search Results

1. Advances in boron essentiality research: Symposium summary

Author:
Coughlin, James R.; Nielsen, Forrest H.
Source:
New aspects of trace element research : Vth Conference of the International Society for Trace Element Research in Humans / edited by M. Abdulla ... [et al.] pp. -
Subject:
Xenopus laevis; embryotoxicity; nutrient deficiencies; health promotion; embryogenesis; boron; mice; nutrient requirements; abnormal development; in vitro studies; Danio rerio; dietary minerals; adverse effects; nutrition research; organogenesis
Handle:
10113/46484

2. The nutritional importance and pharmacological potential of boron for higher animals and human

Author:
Nielsen, Forrest H.
Source:
Boron in plant and animal nutrition / edited by Heiner E. Goldbach ... [et al.] pp. -
Subject:
epidemiological studies; human reproduction; mineral metabolism; Xenopus laevis; nutrient deficiencies; embryogenesis; boron; nutrient requirements; animal models; Danio rerio; dietary minerals; life cycle (organisms); adverse effects; organogenesis
Handle:
10113/46491

3. Metabolism of chlorobenzene and hexachlorobenzene by the zebra fish, Brachydanio rerio

Author:
Kasokat, T.; Nagel, R.; Urich, K.
Source:
Bulletin of environmental contamination and toxicology 1989 v.42 no.2 pp. 254-261
ISSN:
0007-4861
Subject:
water pollution; metabolism; phenols; Danio rerio; benzene
DOI:
10.1007/BF01699408
PubMed:
2920234

4. Toxicity of dimethoate to Daphnia magna and freshwater fish

Author:
Beusen, J.M.; Neven, B.
Source:
Bulletin of environmental contamination and toxicology 1989 v.42 no.1 pp. 126-133
ISSN:
0007-4861
Subject:
water pollution; Poecilia reticulata; dimethoate; insecticide residues; toxicity; Danio rerio
DOI:
10.1007/BF01699213
PubMed:
2923997

5. Acute toxicity of endosulfan to the fish Hyphessobrycon bifasciatus and Brachydanio rerio

Author:
Jonsson, C.M.; Toledo, M.C.F.
Source:
Archives of environmental contamination and toxicology 1993 v.24 no.2 pp. 151-155
ISSN:
0090-4341
Subject:
animal behavior; abnormal behavior; necrosis; environmental exposure; aquatic environment; toxicology; freshwater fish; endosulfan; gills; toxicity; Danio rerio; adverse effects
DOI:
10.1007/BF01141341

6. Testing Acute Toxicity in the Embryo of Zebrafish, Brachydanio rerio, as an Alternative to the Acute Fish Test: Preliminary Results

Author:
Christoph Schulte; Roland Nagel
Source:
ATLA. Alternatives to laboratory animals v.22 no.1 pp. 12-19
ISSN:
0261-1929
Subject:
animal use alternatives; Danio rerio; toxicity; embryo (animal); chemical substances; testing
Abstract:
... Acute toxicity testing in fish is a standard method used in estimating the influences of chemicals on aquatic vertebrates. The ecotoxicological data obtained from acute toxicity tests in fish are, however, not sufficiently reliable to justify the continued use of this test. Fertilised eggs of zebrafish (Brachydanio rerio) were used to test the acute toxicity of chemicals. They were chosen because ...
DOI:
10.1177/026119299402200104

7. Toxicity of 4-chloroaniline in early life-stages of zebrafish (Brachydanio rerio). I. Cytopathology of liver and kidney after microinjection

Author:
Oulmi, Y.; Braunbeck, T.
Source:
Archives of environmental contamination and toxicology 1996 v.30 no.3 pp. 390-402
ISSN:
0090-4341
Subject:
Danio rerio; injection; organochlorine compounds; cytotoxicity; hatching; liver; kidneys; ultrastructure; toxicology; testing; bioassays; animal use alternatives; embryo (animal)
DOI:
10.1007/BF00212299
PubMed:
8854970

8. Construction, electroporatic transfer and expression of ZpβypGH and ZpβrtGH in zebrafish

Author:
S. G. Sheela; J. D. Chen; S. Mathavan; T. J. Pandian
Source:
Journal of biosciences 1998 v.23 no.5 pp. 565-576
ISSN:
0250-5991
Subject:
Danio rerio; Southern blotting; adults; animal growth; beta-galactosidase; capacitance; complementary DNA; electroporation; fins; fish; fish eggs; genetic transformation; genetic vectors; germ cells; parents; promoter regions; reporter genes; somatotropin; transgenes
Abstract:
... Recombinant transformation vectors (ZPβypGH and ZpβrtGH) consisting of fish growth hormone cDNA, and a reporter geneβ-galactosidase driven by fish promoter (Zp) were constructed. Freshly fertilized eggs of zebrafish (Brachydanio rerio) were electroporated at optimum conditions (0.07 kV voltage; 25 μF capacitance; 8 ohm resistance and 2 pulses) in the presence of one of these transformation vectors ...
DOI:
10.1007/BF02709167

9. Molecular cloning of a cDNA coding for copper/zinc superoxide dismutase from zebrafish and its expression in Escherichia coli

Author:
Ken, C.F.; Shaw, J.F.; Wu, J.L.; Lin, C.T.
Source:
Journal of agricultural and food chemistry 1998 v.46 no.7 pp. 2863-2867
ISSN:
0021-8561
Subject:
superoxide dismutase; copper; zinc; Danio rerio; clones; complementary DNA; gene expression; genetic transformation; Escherichia coli; nucleotide sequences; amino acid sequences; sequence alignment
Abstract:
... A full-length complementary DNA (cDNA) clone encoding a putative copper/zinc superoxide dismutase (Cu/Zn-SOD) was amplified by a polymerase chain reaction (PCR)-based technique from cDNA synthesized from zebrafish (Danio rerio) mRNA. Nucleotide sequence analysis of this cDNA clone revealed that it comprised a complete open reading frame coding for 154 amino acid residues. The deduced amino acid se ...
DOI:
10.1021/jf970763e

10. Novel gene expressed during early embryogenesis of zebrafish identified by mRNA differential display

Author:
Li Shuhong; Guo Shaodong; Sun Fangzhen
Source:
Chinese science bulletin 1998 v.43 no.11 pp. 936-940
ISSN:
1001-6538
Subject:
Danio rerio; animal models; complementary DNA; embryo (animal); embryogenesis; gene expression; genes; messenger RNA; morphogenesis; sequence analysis
Abstract:
... Following the revelation of the molecular mechanism of morphogenesis in fruitfly, research on the molecular mechanism of morphogenesis in vertebrate becomes the focus of developmental biology. The isolation of genes controlling the embryogenesis of zebrafish, a vertebrate model animal, is considered as an initial step toward investigating this iasue. There are several approaches that can be used t ...
DOI:
10.1007/BF02884617

11. The response of trout and zebrafish embryos to low and high boron concentrations is U-shaped

Author:
Ruby I. Rowe; Collen Bouzan; Sam Nabili; Curtis D. Eckhert
Source:
Biological trace element research 1998 v.66 no.1-3 pp. 261-270
ISSN:
0163-4984
Subject:
Danio rerio; Oncorhynchus mykiss; adverse effects; boric acid; boron; calcium; calcium carbonate; chronic exposure; death; dose response; parents; trout; water hardness; zygote
Abstract:
... Fish in the embryo-larval stage of development have been shown to be sensitive to boron (B) at both ends of the dose-response curve (1,2). The present study evaluated the health effects of low and high B concentrations on rainbow trout (Oncorhynchus mykiss), a cold water species, and zebrafish (Danio rerio), a warm water species. Rainbow trout embryos were incubated from day 1 until 2 wk posthatch ...
DOI:
10.1007/BF02783142

12. Tc1-Like Transposable Elements in the Genome of Lake Trout (Salvelinus namaycush)

Author:
Kent M. Reed
Source:
Marine biotechnology 1999 v.1 no.1 pp. 60-67
ISSN:
1436-2228
Subject:
DNA primers; Danio rerio; Salvelinus namaycush; amino acids; consensus sequence; fish; genes; polymerase chain reaction; ribosomal DNA; sequence analysis; terminal repeat sequences; transposons
Abstract:
... This study reports on the DNA sequence of a Tc1-like transposable element Tsn1 from lake trout (Salvelinus namaycush). Tc1-like elements were amplified by PCR using an oligonucleotide primer based on the Tdr1 element of zebrafish. One full-length and two partial-length copies of the transposon were sequenced. In addition, partial Tsn1 elements were recovered from PCR reactions run with primers spe ...
DOI:
10.1007/PL00011752

13. Characterization of two microparticle types for delivery of food to altricial fish larvae

Author:
Onal, U.; Langdon, C.
Source:
Aquaculture nutrition 2000 v.6 no.3 pp. 159-170
ISSN:
1353-5773
Subject:
feeds; rearing; polymerization; Artemia; alginates; gelatin; leaching; microencapsulation; solubility; feed intake; larvae; Danio rerio; molecular weight; fish feeding; mortality
DOI:
10.1046/j.1365-2095.2000.00115.x

14. Cloning and expression of a cDNA coding for catalase from zebrafish (Danio rerio)

Author:
Ken, C.F.; Lin, C.T.; Wu, J.L.; Shaw, J.F.
Source:
Journal of agricultural and food chemistry 2000 v.48 no.6 pp. 2092-2096
ISSN:
0021-8561
Subject:
protein secondary structure; interspecific variation; complementary DNA; open reading frames; amino acid sequences; Danio rerio; enzyme activity; molecular conformation
DOI:
10.1021/jf990838+
PubMed:
10888504

15. Conserved protein motifs and structural organization of a fish gene homologous to mammalian apolipoprotein E

Author:
Michèle Durliat; Michèle André; Patrick J. Babin
Source:
European journal of biochemistry 2000 v.267 no.2 pp. 549-559
ISSN:
0014-2956
Subject:
trout; Alzheimer disease; TATA box; cardiovascular diseases; humans; neurodegenerative diseases; DNA; phylogeny; receptors; introns; reverse transcriptase polymerase chain reaction; transcription (genetics); apolipoprotein E; low density lipoprotein; Danio rerio; lipid metabolism; exons; databases
Abstract:
... Apolipoprotein E (apoE) plays a central role in lipid metabolism from its ability to interact with lipoprotein receptors. Besides its role in cardiovascular diseases, apoE polymorphism contributes to susceptibility to neurodegenerative diseases, such as Alzheimer's disease. The statistical significance of the combined match scores obtained after apoE motif‐based protein sequence database searc ...
DOI:
10.1046/j.1432-1327.2000.01033.x

16. Detection of a Spontaneous High Expression of Heat Shock Protein 70 in Developing Zebrafish (Danio rerio)

Author:
Fu-Lung YEH; Todd HSU
Source:
Bioscience, biotechnology, and biochemistry 2000 v.64 no.3 pp. 592-595
ISSN:
1347-6947
Subject:
Danio rerio; biotechnology; electrophoresis; genes; heat stress; heat treatment; heat-shock protein 70; larvae
Abstract:
... A spontaneous high expression of heat shock protein 70 (HSP 70) was detected in zebrafish (Danio rerio) at early larval stage (84 h after fertilization), but the HSP 70 level was either low or barely detectable in 12, 24, 36, 60, and 108 h after fertilization. The extracts of zebrafish at 80 and 84 h after fertilization formed a clear protein-DNA complex with a probe containing heat shock elements ...
DOI:
10.1271/bbb.64.592

17. Field emission scanning electron microscopy and transmission electron microscopy studies of the chorion, plasma membrane syncytial layers of the gastrula-stage embryo of the zebrafish Brachydanio rerio: a consideration of the structural and functional relationships with respect to cryoprotectant penetration

Author:
Rawson, D.M.; Zhang, T.; Kalicharan, D.; Jongebloed, W.L.
Source:
Aquaculture research 2000 v.31 no.3 pp. 325-336
ISSN:
1355-557X
Subject:
semen; cryopreservation; organelles; developmental stages; chorion; cryoprotectants; plasma membrane; Danio rerio; embryogenesis
DOI:
10.1046/j.1365-2109.2000.00401.x

18. Gene-Gun-Mediated Transfer of Reporter Genes to Somatic Zebrafish (Danio rerio) Tissues

Author:
Jacob Torgersen; Philippe Collas; Peter Aleström
Source:
Marine biotechnology 2000 v.2 no.3 pp. 293-300
ISSN:
1436-2228
Subject:
Danio rerio; biolistics; dermis; fluorescence in situ hybridization; green fluorescent protein; luciferase; plasmids; reporter genes; risk factors; spermatozoa
Abstract:
... We describe the use of gene-gun-mediated transfer of luciferase and green fluorescent protein (GFP) reporter genes in zebrafish (Danio rerio). Optimization of DNA transfer parameters indicated highest overall luciferase expression in epidermis and dermis using 1-μm microcarriers and 1 μg of pCMVL plasmid DNA at a delivery pressure of 200 psi. Time course studies revealed luciferase activity peakin ...
DOI:
10.1007/s101260000002
PubMed:
10852809

19. Guidelines for reporting the results of experiments on fish

Author:
Trond Brattelid; Adrian J. Smith
Source:
Laboratory animals 2000 v.34 no.2 pp. 131-135
ISSN:
1758-1117
Subject:
Salmo salar; interspecific variation; water quality; animal husbandry; animal experimentation; laboratory animals; fish; Danio rerio
Abstract:
... A detailed account of experimental design, including an accurate description of the animals used, is an essential part of good research practice. Without these details, the reader will be unable not only to form an opinion on the significance of the findings but also to repeat the experiment in another laboratory. This paper presents suggested guidelines for reporting experimental studies using fi ...
DOI:
10.1258/002367700780457590
PubMed:
10817451

20. Isolation, characterization, expression and functional analysis of the zebrafish ortholog of MEN1

Author:
Pachiappan Manickam; Andreas M. Vogel; Sunita K. Agarwal; Takaya Oda; Allen M. Spiegel; Stephen J. Marx; Francis S. Collins; Brant M. Weinstein; Settara C. Chandrasekharappa
Source:
Mammalian genome 2000 v.11 no.6 pp. 448-454
ISSN:
0938-8990
Subject:
Danio rerio; adults; blood; fish; genes; humans; in situ hybridization; linkage groups; loci; mice; missense mutation; neoplasms; transcription factors
Abstract:
... Mutations in the MEN1 gene lead to an autosomal dominant disorder, multiple endocrine neoplasia type 1 (MEN1), which is characterized by tumors of the parathyroid, entero-pancreatic neuroendocrine, and pituitary tissues. The protein encoded by MEN1, 610-amino acid menin, resides primarily in the nucleus and binds to the transcription factor JunD, resulting in the repression of JunD-induced transcr ...
DOI:
10.1007/s003350010085
PubMed:
10818209

21. The individual tolerance concept is not the sole explanation for the probit dose-effect model

Author:
Newman, M.C.; McCloskey, J.T.
Source:
Environmental toxicology and chemistry 2000 v.19 no.2 pp. 520-526
ISSN:
0730-7268
Subject:
Danio rerio; Gambusia holbrooki; benzocaine; chronic exposure; death; fish; models; pentachlorophenol; prediction; probit analysis; sodium chloride; stochastic processes
Abstract:
... Predominant methods for analyzing dose- or concentration-effect data (i.e., probit analysis) are based on the concept of individual tolerance or individual effective dose (IED, the smallest characteristic dose needed to kill an individual). An alternative explanation (stochasticity hypothesis) is that individuals do not have unique tolerances: death results from stochastic processes occurring simi ...
DOI:
10.1897/1551-5028(2000)019<0520:TITCIN>2.3.CO;2

22. Bax and other pro-apoptotic Bcl-2 family "killer-proteins" and their victim the mitochondrion

Author:
Bruno Antonsson
Source:
Cell and tissue research 2001 v.306 no.3 pp. 347-361
ISSN:
0302-766X
Subject:
Danio rerio; Drosophila; apoptosis; caspases; cytochrome c; cytosol; death; mammals; mitochondria; post-translational modification; signal transduction
Abstract:
... Two major intracellular apoptosis signaling cascades have been characterized, the mitochondrial pathway and the death receptor pathway. The mitochondrial pathway is regulated by members of the Bcl-2 protein family. The members of this family can be subdivided into anti- and pro-apoptotic proteins. The pro-apoptotic members are further divided into two groups, the multidomain and the 'BH3 domain on ...
DOI:
10.1007/s00441-001-0472-0
PubMed:
11735035

23. Bioaccumulation of Selected PCBs in Zebrafish, Three-Spined Stickleback, and Arctic Char After Three Different Routes of Exposure

Author:
P. L. Andersson; A. H. Berg; R. Bjerselius; L. Norrgren; H. Olsén; P.-E. Olsson; S. Örn; M. Tysklind
Source:
Archives of environmental contamination and toxicology 2001 v.40 no.4 pp. 519-530
ISSN:
0090-4341
Subject:
Danio rerio; Gasterosteus aculeatus; Salvelinus alpinus; bioaccumulation; correlation; diet; exposure pathways; fish; hydrophobicity; intraperitoneal injection; peanut oil; polychlorinated biphenyls; silicone
Abstract:
... The uptake and elimination of 20 structurally diverse tetra- to heptachlorinated biphenyls were studied in zebrafish (Danio rerio), three-spined stickleback (Gasterosteus aculeatus), and Arctic char (Salvelinus alpinus). The polychlorinated biphenyls (PCBs) were administered to the fish through food, intraperitoneal injection of peanut oil, or intraperitoneal implantation of silicone capsules. The ...
DOI:
10.1007/s002440010205

24. Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

Author:
Vikas Anathy; Thayanithy Venugopal; Ramanathan Koteeswaran; Thavamani J. Pandian; Sinnakaruppan Mathavan
Source:
Journal of biosciences 2001 v.26 no.3 pp. 315-324
ISSN:
0250-5991
Subject:
Danio rerio; Heteropneustes fossilis; RNA; amino acids; bacteriophages; cDNA libraries; catfish; complementary DNA; fossils; open reading frames; polymerase chain reaction; polypeptides; signal peptide; somatotropin; viability
Abstract:
... A tissue-specific cDNA library was constructed using polyA⁺ RNA from pituitary glands of the Indian catfishHeteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3′ region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the c ...
DOI:
10.1007/BF02703740
PubMed:
11568476

25. Development of sensory systems in zebrafish (Danio rerio)

Author:
Moorman, S.J.
Source:
ILAR journal 2001 v.42 no.4 pp. 292-298
ISSN:
1930-6180
Subject:
chemoreceptors; cell differentiation; peripheral nerves; neurons; laboratory animals; gene expression; smell; animal models; retina; mutants; Danio rerio; embryogenesis
DOI:
10.1093/ilar.42.4.292
PubMed:
11581521

26. Immunocytochemical detection of Reissner's fiber-like glycoproteins in the subcommissural organ and the floor plate of wildtype and cyclops mutant zebrafish larvae

Author:
P. Fernández-Llebrez; S. Hernández; J. Andrades
Source:
Cell and tissue research 2001 v.305 no.1 pp. 115-120
ISSN:
0302-766X
Subject:
Danio rerio; antiserum; brain; cattle; genes; glycoproteins; larvae; molecular weight; mutants; polymerization; spinal cord; tail
Abstract:
... The subcommissural organ (SCO) and the floor plate (FP) secrete high molecular weight glycoproteins that polymerize in the form of the Reissner's fiber (RF). To study to what extent the absence of the FP affects the expression of these glycoproteins, we have investigated the brain and spinal cord of 48-h and 72-h wildtype and cyclops (cyc) mutant zebrafish larvae by using a polyclonal antiserum ag ...
DOI:
10.1007/s004410100404
PubMed:
11512663

27. Mechanistic considerations in small fish carcinogenicity testing

Author:
Law, J.M.
Source:
ILAR journal 2001 v.42 no.4 pp. 274-284
ISSN:
1930-6180
Subject:
fish; laboratory animals; bioassays; carcinogenesis; toxic substances; carcinogenicity; Oryzias latipes; Danio rerio; mutagenesis; immunohistochemistry; experimental design; oncogenes; animal models
DOI:
10.1093/ilar.42.4.274
PubMed:
11581519

28. Reproductive Effects of Ethynylestradiol and 4t-Octylphenol on the Zebrafish (Danio rerio)

Author:
K. Van den Belt; R. Verheyen; H. Witters
Source:
Archives of environmental contamination and toxicology 2001 v.41 no.4 pp. 458-467
ISSN:
0090-4341
Subject:
Danio rerio; adults; blood proteins; females; fish; gonadosomatic index; males; reproduction; spawning; testes; vitellogenin
Abstract:
... In this study, the impact of ethynylestradiol (EE2) and 4t-octylphenol (OP) on reproduction in zebrafish (Danio rerio) was evaluated using spawning and fertilization success, gonadosomatic index, and plasma vitellogenin (VTG) levels as endpoints. Adult male and female zebrafish were exposed under semi-static conditions to 5, 10, 25 and 50 ng/L EE2 and to 12.5, 25, 50 and 100 μg/L OP for 3 weeks. A ...
DOI:
10.1007/s002440010272
PubMed:
11598783

29. A neuropeptide Y receptor Y1‐subfamily gene from an agnathan, the European river lamprey: A potential ancestral gene

Author:
Erik Salaneck; Robert Fredriksson; Earl T. Larson; J. Michael Conlon; Dan Larhammar
Source:
European journal of biochemistry 2001 v.268 no.23 pp. 6146-6154
ISSN:
0014-2956
Subject:
Danio rerio; Lampetra fluviatilis; amino acid sequences; binding properties; central nervous system; chromosomes; genes; mammals; messenger RNA; neuropeptide Y; peptide YY; phylogeny; receptors; reverse transcriptase polymerase chain reaction; sequence analysis
Abstract:
... We report here the isolation and functional expression of a neuropeptide Y (NPY) receptor from the river lamprey, Lampetra fluviatilis. The receptor displays ≈ 50% amino‐acid sequence identity to all previously cloned Y1‐subfamily receptors including Y1, Y4, and y6 and the teleost subtypes Ya, Yb and Yc. Phylogenetic analyses point to a closer relationship with Y4 and Ya/b/c suggesting t ...
DOI:
10.1046/j.0014-2956.2001.02561.x

30. The toxicity of the antiparasitic mixture FMC is changed by humic substances and calcium

Author:
Meinelt, T.; Playle, R.; Pietrock, M.
Source:
Aquaculture research 2001 v.32 no.5 pp. 405-410
ISSN:
1355-557X
Subject:
Achlya; water quality; ova; fungal diseases of plants; methylene blue; calcium; humic acids; synergism; malachite green; toxicity; Danio rerio; mixtures; Saprolegnia; formaldehyde; inorganic ions
Abstract:
... An inexpensive mixture of the therapeutic agents malachite green (MAG), methylene blue (MB) and formalin (known as FMC) is used to control external fungal infections in fish eggs (e.g. Saprolegnia ssp., Achlya ssp.) in aquaculture and ornamental fish breeding. The treatment success as well as the toxicity of the chemicals is determined by the toxicity of each of the substances and synergistic effe ...
DOI:
10.1046/j.1365-2109.2001.00576.x

31. A zebrafish histone variant H2A.F/Z and a transgenic H2A.F/Z:GFP fusion protein for in vivo studies of embryonic development

Author:
Stefan Pauls; Benedikt Geldmacher-Voss; José A. Campos-Ortega
Source:
Development genes and evolution 2001 v.211 no.12 pp. 603-610
ISSN:
0949-944X
Subject:
Aequorea victoria; Danio rerio; Scyphozoa; cytoplasm; eggs; embryogenesis; fish; genes; germ cells; green fluorescent protein; histones; in vivo studies; molecular cloning
Abstract:
... We have generated transgenic zebrafish lines expressing a fusion of a histone variant, H2A.F/Z, to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria. Here, we describe the molecular cloning, partial characterisation and expression of the zebrafish H2A.F/Z histone gene, as well as the construction of the transgene and its transformation into the zebrafish germ line. No abnormal ...
DOI:
10.1007/s00427-001-0196-x
PubMed:
11819118

32. Cellular retinol‐binding protein type II (CRBPII) in adult zebrafish (Danio rerio): cDNA sequence, tissue‐specific expression and gene linkage analysis

Author:
Marianne C. Cameron; Eileen M. Denovan‐Wright; Mukesh K. Sharma; Jonathan M. Wright
Source:
European journal of biochemistry 2002 v.269 no.18 pp. 4685-4692
ISSN:
0014-2956
Subject:
Danio rerio; adults; autoradiography; chickens; complementary DNA; emulsions; fatty acid-binding proteins; fish; gene expression; genes; humans; in situ hybridization; intestines; liver; messenger RNA; metabolism; nucleotide sequences; radiation hybrid mapping; retinol-binding protein; vitamin A
Abstract:
... We have determined the nucleotide sequence of a zebrafish cDNA clone that codes for a cellular retinol‐binding protein type II (CRBPII). Radiation hybrid mapping revealed that the zebrafish and human CRBPII genes are located in syntenic groups. In situ hybridization and emulsion autoradiography localized the CRBPII mRNA to the intestine and the liver of adult zebrafish. CRBPII and intestinal fat ...
DOI:
10.1046/j.1432-1033.2002.03170.x

33. Clock mechanisms in zebrafish

Author:
Gregory M. Cahill
Source:
Cell and tissue research 2002 v.309 no.1 pp. 27-34
ISSN:
0302-766X
Subject:
Danio rerio; circadian rhythm; embryogenesis; genes; genetic techniques and protocols; heart; kidneys; mammals; melatonin; mutants; oocytes; phototransduction
Abstract:
... Recent research on the circadian system of the zebrafish is reviewed. This teleost has become an attractive model system because of its advantages for genetic analyses. Circadian rhythms of zebrafish behavior, visual system function, and pineal melatonin synthesis have been described, and behavioral and pineal rhythms are being used to identify and characterize clock mutants. Zebrafish heart, kidn ...
DOI:
10.1007/s00441-002-0570-7
PubMed:
12111534

34. Functional Analysis of the Proximal Promoter Regions of Fish Rhodopsin and Myf-5 Genes Using Transgenesis

Author:
Tzu-Ming Wang; Yau-Hung Chen; Chia-Feng Liu; Huai-Jen Tsai
Source:
Marine biotechnology 2002 v.4 no.3 pp. 247-255
ISSN:
1436-2228
Subject:
Cyprinus carpio; Danio rerio; carp; gene expression; genetically modified organisms; green fluorescent protein; myocytes; promoter regions; retina; rhodopsin; transcription (genetics); transgenes; transposons
Abstract:
... : Little is known about the cis-acting elements controlling transcriptional activities of fish rhodopsin and myf-5 genes. Transgenic medaka and zebrafish were used to characterize promoters of carp (Cyprinus carpio) rhodopsin gene (cRh) and zebrafish (Danio rerio) myf-5 gene (myf-5), respectively. Transgenic medaka carrying different lengths of cRh upstream fragments fused with enhanced green fluo ...
DOI:
10.1007/s10126-002-0016-y

35. Gene Expression of Insulin-like Growth Factor-I Receptor and p53 Suppressor during Zebrafish (Danio rerio) Embryogenesis

Author:
Ronshan Cheng; Jen-Leih Wu
Source:
Marine biotechnology 2002 v.4 no.3 pp. 226-235
ISSN:
1436-2228
Subject:
Danio rerio; amino acids; bioactive properties; clones; developmental stages; embryogenesis; frogs; gene expression; head; humans; in situ hybridization; insulin-like growth factor I; insulin-like growth factor I receptor; messenger RNA; mice; nucleotides; reverse transcriptase polymerase chain reaction; suppressor genes
Abstract:
... : In vertebrates insulin-like growth factors (IGFs) regulate important cellular activities involving proliferation, differentiation, and antiapoptosis and their biological activities are mediated through the insulin-like growth factor-I receptor (IGF-IR). To understand the functions of IGF-IR in zebrafish embryogenesis, the polymerase chain reaction (PCR) cloning technique was applied to isolate t ...
DOI:
10.1007/s10126-002-0015-z

36. Generation of Two-color Transgenic Zebrafish Using the Green and Red Fluorescent Protein Reporter Genes gfp and rfp

Author:
Haiyan Wan; Jiangyan He; Bensheng Ju; Tie Yan; Toong Jin Lam; Zhiyuan Gong
Source:
Marine biotechnology 2002 v.4 no.2 pp. 146-154
ISSN:
1436-2228
Subject:
Danio rerio; detection limit; epithelium; fluorescence; genetically modified organisms; green fluorescent protein; monitoring; muscle tissues; muscles; myocytes; myosin light chains; polypeptides; progeny; protein synthesis; reporter genes
Abstract:
... Two tissue-specific promoters were used to express both green fluorescent protein (GFP) and red fluorescent protein (RFP) in transgenic zebrafish embryos. One promoter (CK), derived from a cytokeratin gene, is active specifically in skin epithelia in embryos, and the other promoter (MLC) from a muscle-specific gene encodes a myosin light chain 2 polypeptide. When the 2 promoters drove the 2 report ...
DOI:
10.1007/s10126-001-0085-3

37. Iodine species and the endocrine system: thyroid hormone levels in adult Danio rerio and developing Xenopus laevis

Author:
R. Simon; J. Tietge; B. Michalke; S. Degitz; K.-W. Schramm
Source:
Analytical and bioanalytical chemistry 2002 v.372 no.3 pp. 481-485
ISSN:
1618-2642
Subject:
Danio rerio; Xenopus laevis; adults; blood serum; endocrine system; females; humans; iodides; iodine; liquid chromatography; males; mass spectrometry; tadpoles; thyroid hormones
Abstract:
... Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography–inductively coupled plasma–mass spectrometry (LC–ICP–MS). This method enables quantification of iodide, T₄and T₃, as well as reverse T₃(rT₃) and the synthetic precursors of TH, monoiodotyrosine (MIT), and diiodotyrosine (DIT) in a single injection. In this work, the L ...
DOI:
10.1007/s00216-001-1211-9

38. Opponent and nonopponent contributions to the zebrafish electroretinogram using heterochromatic flicker photometry

Author:
W. Patterson; A. McDowell; A. Hughes; J. Bilotta
Source:
Journal of comparative physiology 2002 v.188 no.4 pp. 283-293
ISSN:
0340-7594
Subject:
Danio rerio; electroretinography; vertebrates; vision
Abstract:
... While some lower vertebrates, such as zebrafish, do not appear to possess anatomically separate pathways of processing visual information (such as M-pathways and P-pathways), it is believed that separate processing of the visual stimulus (such as luminance and chromatic processing) is a basic requirement of vertebrate vision. In this study, spectral sensitivity functions were obtained from electro ...
DOI:
10.1007/s00359-002-0302-1
PubMed:
12012099

39. A functionally conserved member of the FTZ‐F1 nuclear receptor family from Schistosoma mansoni

Author:
Ricardo L de Mendonça; Didier Bouton; Benjamin Bertin; Hector Escriva; Christophe Noël; Jean‐Marc Vanacker; Jocelyne Cornette; Vincent Laudet; Raymond J. Pierce
Source:
European journal of biochemistry 2002 v.269 no.22 pp. 5700-5711
ISSN:
0014-2956
Subject:
DNA-binding domains; Danio rerio; Drosophila; Schistosoma mansoni; adults; cercariae; exons; functional properties; humans; invertebrates; larvae; males; messenger RNA; miracidia; mutants; parasites; phylogeny; protein synthesis; receptors; reporter genes; sexual development; transcription (genetics)
Abstract:
... The fushi tarazu factor 1 (FTZ‐F1) nuclear receptor subfamily comprises orphan receptors with crucial roles in development and sexual differentiation in vertebrates and invertebrates. We describe the structure and functional properties of an FTZ‐F1 from the platyhelminth parasite of humans, Schistosoma mansoni, the first receptor from this family to be characterized in a Lophotrochozoan. It co ...
DOI:
10.1046/j.1432-1033.2002.03287.x

40. Baculovirus-Mediated Gene Expression in Zebrafish

Author:
Mahendra Wagle; Suresh Jesuthasan
Source:
Marine biotechnology 2003 v.5 no.1 pp. 58-63
ISSN:
1436-2228
Subject:
Baculoviridae; Danio rerio; gene expression; genes; tissues; viruses
Abstract:
... : AbstractIn an effort to misexpress genes in zebrafish, we tested the ability of baculovirus to infect and drive gene expression in embryos. By injecting virus into specific tissues and using appropriate promoters, both the location and time of gene expression could be controlled. Using a virus with 2 different promoters, LacZ and GFP could be expressed independently. The efficiency of expression ...
DOI:
10.1007/s10126-002-0050-9

41. Characterization of hey bHLH genes in teleost fish

Author:
Christoph Winkler; Harun Elmasri; Barbara Klamt; Jean-Nicolas Volff; Manfred Gessler
Source:
Development genes and evolution 2003 v.213 no.11 pp. 541-553
ISSN:
0949-944X
Subject:
Tetraodon nigroviridis; freshwater; transcription factors; polymerase chain reaction; phylogeny; gene expression; mice; heart; genes; mutants; blood vessels; fish; Danio rerio; embryogenesis; Takifugu rubripes; databases
Abstract:
... Hairy-related basic helix-loop-helix (bHLH) transcription factors are targets of Delta-Notch signaling and represent essential components for a number of cell fate decisions during vertebrate embryogenesis. Hey genes encode a subfamily of hairy-related proteins that have been implicated in processes like somitogenesis, blood vessel and heart development. We have identified and characterized hey ge ...
DOI:
10.1007/s00427-003-0360-6

42. Comparative analysis of the mitochondrial DNA control region in cyprinids with different ploidy level. [Erratum: 2004 Apr. 26, v. 233, no. 1-4, p. 615-617.]

Author:
Guo, X.; Liu, S.; Liu, Y.
Source:
Aquaculture 2003 v.224 no.1-4 pp. 25-38
ISSN:
0044-8486
Subject:
Carassius; Cyprinus carpio; carp; Danio rerio; hybrids; fish culture; mitochondrial DNA; cytogenetic analysis; polymerase chain reaction; DNA primers; nucleotide sequences; allopolyploidy; diploidy; triploidy; tetraploidy; sequence alignment; sequence homology; DNA-binding domains; genetic markers; China
Abstract:
... The comprehensive analysis of the complete mitochondrial DNA control region (mtDNA-CR) sequences from the different ploidy level cyprinid fish was made. The mtDNA-CR sequences for allotriploid crucian carp and allotriploid common carp were amplified by the polymerase chain reaction (PCR) and the amplified products were sequenced. The mtDNA-CR sequences from diploids, such as the red crucian carp, ...

43. Survival of zebrafish, Brachydanio rerio (Hamilton-Buchanan), embryo after immersion in methanol and exposure to ultrasound with implications to cryopreservation

Author:
Bart, A.N.; Kyaw, H.A.
Source:
Aquaculture research 2003 v.34 no.8 pp. 609-615
ISSN:
1355-557X
Subject:
Danio rerio; embryo (animal); embryogenesis; cryoprotectants; methanol; ultrasonic treatment; cryopreservation
Abstract:
... This study examined the viability of embryos after immersion in highly concentrated methanol solutions (40-60%) and exposing embryos to ultrasound to enhance efficient transport of the cryoprotectant. The exposure to ultrasound, methanol concentrations, duration of treatment and the stages of embryonic development was found to have measurable effects on embryo viability. The effect of ultrasound w ...
DOI:
10.1046/j.1365-2109.2003.00852.x

44. Comparative study of microcystin-LR-induced behavioral changes of two fish species, Danio rerio and Leucaspius delineatus

Author:
Baganz, D.; Staaks, G.; Pflugmacher, S.; Steinberg, C.E.W.
Source:
Environmental toxicology 2004 v.19 no.6 pp. 564-570
ISSN:
1520-4081
Subject:
Danio rerio; chronic exposure; diurnal activity; fish; microcystin-LR; nocturnal activity; predator-prey relationships; reproduction; swimming; tanks
Abstract:
... The spontaneous locomotor behavior separated into day/night activity phases of two fish species Danio rerio and Leucaspius delineatus was recorded and quantified continuously under sublethal long-term exposure to microcystin-LR in tanks. Microcystin-LR was applied in concentrations of 0.5, 5, 15, and 50 microgram L-1. By using an automated video-monitoring and object-tracing system, the average mo ...
DOI:
10.1002/tox.20063
PubMed:
15526272

45. Lipid spray beads for delivery of riboflavin to first-feeding fish larvae

Author:
Onal, U.; Langdon, C.
Source:
Aquaculture 2004 v.233 no.1-4 pp. 477-493
ISSN:
0044-8486
Subject:
Danio rerio; freshwater fish; fish larvae; feeds; artificial diets; riboflavin; fish feeding; fish culture; freshwater aquaculture; emulsifiers; lipids; palmitates; dose response; waxes
Abstract:
... High nutrient loss due to leakage from microparticulate diets is an important problem in the development of artificial diets for marine fish larvae. Lipid spray beads (LSB) are promising as they can be used to deliver low-molecular weight, water-soluble nutrients. Here, we describe a simple method for the production of LSB composed of methyl palmitate (MP), a low melting point wax, for delivering ...

46. Small fish models for identifying and assessing the effects of endocrine-disrupting chemicals

Author:
Ankley, G.T.; Johnson, R.D.
Source:
ILAR journal 2004 v.45 no.4 pp. 469-483
ISSN:
1930-6180
Subject:
laboratory animals; animal models; Danio rerio; bioassays
DOI:
10.1093/ilar.45.4.469
PubMed:
15454686

47. [beta]-Arrestin 2 Regulates Zebrafish Development Through the Hedgehog Signaling Pathway

Author:
Wilbanks, Alyson M.; Fralish, Gregory B.; Kirby, Margaret L.; Barak, Larry S.; Li, Yin-Xiong; Caron, Marc G.
Source:
Science 2004 v.306 no.5705 pp. 2264-2267
ISSN:
0036-8075
Subject:
Danio rerio; mutants; phenotype; receptors; signal transduction
Abstract:
... [beta]-arrestins are multifunctional proteins that act as scaffolds and transducers of intracellular signals from heptahelical transmembrane-spanning receptors (7TMR). Hedgehog (Hh) signaling, which uses the putative 7TMR, Smoothened, is established as a fundamental pathway in development, and unregulated Hh signaling is associated with certain malignancies. Here, we show that the functional knock ...
DOI:
10.1126/science.1104193

48. Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies

Author:
Grewal, Prabhjit K.; McLaughlan, Jennifer M.; Moore, Christopher J.; Browning, Claudia A.; Hewitt, Jane E.
Source:
Glycobiology 2005 v.15 no.10 pp. 912-923
ISSN:
0959-6658
Subject:
Danio rerio; Golgi apparatus; Tetraodontidae; chickens; databases; dogs; genes; glycosylation; glycosyltransferases; humans; invertebrates; kidneys; laminin; ligands; loss-of-function mutation; messenger RNA; mice; muscular dystrophy; mutants; myoblasts; reverse transcriptase polymerase chain reaction
Abstract:
... The Large[superscript myd] mouse has a loss-of-function mutation in the putative glycosyltransferase gene LARGE: Mutations in the human homolog (LARGE) have been described in a form of congenital muscular dystrophy (MDC1D). Other genes (POMT1, POMGnT1, fukutin, and FKRP) that encode known or putative glycosylation enzymes are also causally associated with human congenital muscular dystrophies. All ...
DOI:
10.1093/glycob/cwi094

49. Conservation of novel Mahya genes shows the existence of neural functions common between Hymenoptera and Deuterostome

Author:
Tsuchimoto, Mayumi; Yasuo, Shinobu; Funada, Masahiro; Aoki, Makoto; Sasagawa, Hiromi; Yoshimura, Takashi; Tadauchi, Osamu; Cameron, Sydney A.; Kitagawa, Yasuo; Kadowaki, Tatsuhiko
Source:
Development genes and evolution 2005 v.215 no.11 pp. 564-574
ISSN:
0949-944X
Subject:
Apis mellifera; Culicidae; Danio rerio; Lepidoptera; Nematoda; cerebellum; cognition; evolution; fruit flies; genes; hippocampus; honey bees; immunoglobulins; life history; memory; mice; moths; mushroom bodies; olfactory bulb; proteinase inhibitors
Abstract:
... Honeybees have been shown to exhibit cognitive performances that were thought to be specific to some vertebrates. However, the molecular and cellular mechanisms of such cognitive abilities of the bees have not been understood. We have identified a novel gene, Mahya, expressed in the brain of the honeybee, Apis mellifera, and other Hymenoptera. Mahya orthologues are present in Deuterostomes but are ...
DOI:
10.1007/s00427-005-0021-z
PubMed:
16193321

50. Dissolved saxitoxin causes transient inhibition of sensorimotor function in larval Pacific herring (Clupea harengus pallasi)

Author:
Kathi A. Lefebvre; Nancy E. Elder; Paul K. Hershberger; Vera L. Trainer; Carla M. Stehr; Nathaniel L. Scholz
Source:
Marine biology 2005 v.147 no.6 pp. 1393-1402
ISSN:
0025-3162
Subject:
Alexandrium catenella; Clupea pallasii pallasii; Danio rerio; bioavailability; fish communities; fish larvae; herring; marine fish; neurotoxins; paralysis; paralytic shellfish poisoning; paralytic shellfish toxins; phycotoxins; spawning; sublethal effects; swimming; Puget Sound; Washington (state)
Abstract:
... Herring (Clupea harengus pallasi) spawning sites in Puget Sound, Washington overlap spatially and temporally with blooms of Alexandrium catenella, a toxic dinoflagellate species responsible for paralytic shellfish poisoning. Consequently, newly hatched herring larvae may be regularly exposed to the suite of dissolved paralytic shellfish toxins that are released into the water column from toxic cel ...
DOI:
10.1007/s00227-005-0048-8

51. Enhancement of EPA and DHA biosynthesis by over-expression of masu salmon delta6-desaturase-like gene in zebrafish

Author:
Alimuddin; Yoshizaki, G.; Kiron, V.; Satoh, S.; Takeuchi, T.
Source:
Transgenic research 2005 v.14 no.2 pp. 159-165
ISSN:
0962-8819
Subject:
genetic transformation; gene transfer; messenger RNA; transgenes; gene overexpression; eicosapentaenoic acid; docosahexaenoic acid; fatty acid composition; transgenic animals; lipogenesis; Oncorhynchus masou; Danio rerio; enzyme activity; stearoyl-CoA desaturase
DOI:
10.1007/s11248-004-7435-7
PubMed:
16022387

52. Evolution of New Hormone Function: Loss and Gain of a Receptor

Author:
Irwin, D. M.; Wong, K.
Source:
Journal of heredity 2005 v.96 no.3 pp. 205-211
ISSN:
0022-1503
Subject:
Danio rerio; Tetraodontidae; Xenopus tropicalis; chickens; frogs; genes; glucagon; glucagon receptors; glucagon-like peptide 1; glucagon-like peptide 2; goldfish; mammals; metabolism; phylogeny; secretin
Abstract:
... The vertebrate proglucagon gene encodes three glucagon-like sequences (glucagon, glucagon-like peptide-1 [GLP-1], and glucagon-like peptide 2 [GLP-2]) that have distinct functions in regulating metabolism in mammals. In contrast, glucagon and GLP-1 have similar physiological actions in fish, that of mammalian glucagon. We have identified sequences similar to receptors for proglucagon-derived pepti ...
DOI:
10.1093/jhered/esi024

53. MicroRNA Expression in Zebrafish Embryonic Development

Author:
Wienholds, Erno; Kloosterman, Wigard P.; Miska, Eric; Alvarez-Saavedra, Ezequiel; Berezikov, Eugene; de Bruijn, Ewart; Horvitz, H. Robert; Kauppinen, Sakari; Plasterk, Ronald H. A.
Source:
Science 2005 v.309 no.5732 pp. 310-311
ISSN:
0036-8075
Subject:
Danio rerio; early development; embryogenesis; gene expression; messenger RNA; microRNA; microarray technology; non-coding RNA; nucleotides; oligonucleotide probes; vertebrates
Abstract:
... MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using ...
DOI:
10.1126/science.1114519

54. MicroRNAs Regulate Brain Morphogenesis in Zebrafish

Author:
Giraldez, Antonio J.; Cinalli, Ryan M.; Glasner, Margaret E.; Enright, Anton J.; Thomson, J. Michael; Baskerville, Scott; Hammond, Scott M.; Bartel, David P.; Schier, Alexander F.
Source:
Science 2005 v.308 no.5723 pp. 833-838
ISSN:
0036-8075
Subject:
Danio rerio; brain; gastrulation; gene expression; gene silencing; heart; microRNA; morphogenesis; mutants; ribonucleases
Abstract:
... MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indica ...
DOI:
10.1126/science.1109020

55. Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio)

Author:
Cotto, Emmanuelle; André, Michèle; Forgue, Jean; Fleury, Hervé J.; Babin, Patrick J.
Source:
FEBS journal 2005 v.272 no.2 pp. 500-513
ISSN:
1742-464X
Subject:
Danio rerio; amino acid sequences; brain; eyes; fins; fish; ganglia; genes; heart; humans; in situ hybridization; intestines; kidneys; larvae; larval development; liver; messenger RNA; neuromasts; phylogeny; prion diseases; prions
Abstract:
... Prion diseases are characterized by the accumulation of a pathogenic misfolded form of a prion protein (PrP) encoded by the Prnp gene in humans. In the present study in zebrafish, two transcripts and the corresponding genes encoding prion proteins, PrP1 and PrP2, related to human PrP have been characterized with a relatively divergent deduced amino acid sequence, but a well preserved overall organ ...
DOI:
10.1111/j.1742-4658.2004.04492.x

56. Otolith size-at-hatch reveals embryonic oxygen consumption in the zebrafish, Danio rerio

Author:
A. Bang; P. Grønkjær
Source:
Marine biology 2005 v.147 no.6 pp. 1419-1423
ISSN:
0025-3162
Subject:
Danio rerio; adults; eggs; fish larvae; juveniles; life history; metabolism; otoliths; oxygen consumption; variance; viability
Abstract:
... Otoliths have frequently been used to reconstruct growth histories in larval, juvenile and adult fish. However, there is growing evidence that otolith growth is directly determined by metabolic intensity and, consequently, only indirectly related to somatic growth. By performing measurements of oxygen consumption rate and other early life-history traits on individual eggs of zebrafish (Danio rerio ...
DOI:
10.1007/s00227-005-0037-y

57. Retinoic Acid Signaling Restricts the Cardiac Progenitor Pool

Author:
Keegan, Brian R.; Feldman, Jessica L.; Begemann, Gerrit; Ingham, Philip W.; Yelon, Deborah
Source:
Science 2005 v.307 no.5707 pp. 247-249
ISSN:
0036-8075
Subject:
Danio rerio; cardiomyocytes; organogenesis; retinoic acid; stem cells
Abstract:
... Organogenesis begins with specification of a progenitor cell population, the size of which provides a foundation for the organ's final dimensions. Here, we present a new mechanism for regulating the number of progenitor cells by limiting their density within a competent region. We demonstrate that retinoic acid signaling restricts cardiac specification in the zebrafish embryo. Reduction of retinoi ...
DOI:
10.1126/science.1101573

58. SLC24A5, a Putative Cation Exchanger, Affects Pigmentation in Zebrafish and Humans

Author:
Lamason, Rebecca L.; Mohideen, Manzoor-Ali P.K.; Mest, Jason R.; Wong, Andrew C.; Norton, Heather L.; Aros, Michele C.; Jurynec, Michael J.; Mao, Xianyun; Humphreville, Vanessa R.; Humbert, Jasper E.; Sinha, Soniya; Moore, Jessica L.; Jagadeeswaran, Pudur; Zhao, Wei; Ning, Gang; Makalowska, Izabela; McKeigue, Paul M.; O'Donnell, David; Kittles, Rick; Parra, Esteban J.; Mangini, Nancy J.; Grunwald, David J.; Shriver, Mark D.; Canfield, Victor A.; Cheng, Keith C.
Source:
Science 2005 v.310 no.5755 pp. 1782-1786
ISSN:
0036-8075
Subject:
Danio rerio; alleles; cations; heterozygosity; humans; melanocytes; mutants; organelles; pigmentation
Abstract:
... Lighter variations of pigmentation in humans are associated with diminished number, size, and density of melanosomes, the pigmented organelles of melanocytes. Here we show that zebrafish golden mutants share these melanosomal changes and that golden encodes a putative cation exchanger slc24a5 (nckx5) that localizes to an intracellular membrane, likely the melanosome or its precursor. The human ort ...
DOI:
10.1126/science.1116238

59. Specific RNAi Mediated Gene Knockdown in Zebrafish Cell Lines

Author:
Jens Gruber; Heiko Manninga; Thomas Tuschl; Mary Osborn; Klaus Weber
Source:
RNA biology 2005 v.2 no.3 pp. 101-105
ISSN:
1555-8584
Subject:
Danio rerio; RNA interference; abnormal development; adults; cell lines; death; embryo (animal); gene targeting; genes; mammals; molecular motor proteins; phenotype; small interfering RNA; transfection
Abstract:
... Here we demonstrate highly efficient RNA interference in ZFL, SJD and ZF4 cell lines derived from adult and embryonic zebrafish Danio rerio. Microinjection of siRNAs resulted in silencing in almost 100% of cells while transfection using cationic liposomes led to silencing in 30%. Use of siRNAs against zebrafish lamin A, lamin B2 and the motor protein Eg5, led to knockdown of the target genes with ...
DOI:
10.4161/rna.2.3.2060

60. TAZ, a Transcriptional Modulator of Mesenchymal Stem Cell Differentiation

Author:
Hong, Jeong-Ho; Hwang, Eun Sook; McManus, Michael T.; Amsterdam, Adam; Tian, Yu; Kalmukova, Ralitsa; Mueller, Elisabetta; Benjamin, Thomas; Spiegelman, Bruce M.; Sharp, Phillip A.; Hopkins, Nancy; Yaffe, Michael B.
Source:
Science 2005 v.309 no.5737 pp. 1074-1078
ISSN:
0036-8075
Subject:
Danio rerio; adipocytes; cell differentiation; fibroblasts; mice; models; osteoblasts; peroxisome proliferator-activated receptors; stem cells; transcription (genetics)
Abstract:
... Mesenchymal stem cells (MSCs) are a pluripotent cell type that can differentiate into several distinct lineages. Two key transcription factors, Runx2 and peroxisome proliferator-activated receptor [gamma] (PPAR[gamma]), drive MSCs to differentiate into either osteoblasts or adipocytes, respectively. How these two transcription factors are regulated in order to specify these alternate cell fates re ...
DOI:
10.1126/science.1110955

61. fgf20 Is Essential for Initiating Zebrafish Fin Regeneration

Author:
Whitehead, Geoffrey G.; Makino, Shinji; Lien, Ching-Ling; Keating, Mark T.
Source:
Science 2005 v.310 no.5756 pp. 1957-1960
ISSN:
0036-8075
Subject:
Danio rerio; epithelium; loss-of-function mutation; mutants
Abstract:
... Epimorphic regeneration requires the presence or creation of pluripotent cells capable of reproducing lost organs. Zebrafish fin regeneration is mediated by the creation of blastema cells. Here, we characterize the devoid of blastema (dob) mutant that fails fin regeneration during initial steps, forms abnormal regeneration epithelium, and does not form blastema. This mutation has no impact on embr ...
DOI:
10.1126/science.1117637

62. kitb, a second zebrafish ortholog of mouse Kit

Author:
Mellgren, Eve M.; Johnson, Stephen L.
Source:
Development genes and evolution 2005 v.215 no.9 pp. 470-477
ISSN:
0949-944X
Subject:
Danio rerio; blood; ganglia; gene expression; genes; germ cells; hematopoiesis; mice; neurons; receptor protein-tyrosine kinase
Abstract:
... The large numbers of duplicated pairs of genes in zebrafish compared to their mammalian counterparts has lead to the notion that expression of zebrafish co-orthologous pairs in some cases can together describe the expression of their mammalian counterpart. Here, we explore this notion by identification and analysis of a second zebrafish ortholog of the mammalian Kit receptor tyrosine kinase (kitb) ...
DOI:
10.1007/s00427-005-0001-3
PubMed:
16096802

63. Acute toxicity of ionic liquids to the zebrafish (Danio rerio)

Author:
Carlo Pretti; Cinzia Chiappe; Daniela Pieraccini; Michela Gregori; Francesca Abramo; Gianfranca Monni; Luigi Intorre
Source:
Green chemistry 2006 v.8 no.3 pp. 238-240
ISSN:
1463-9270
Subject:
Danio rerio; acute toxicity; green chemistry; histology; ionic liquids
Abstract:
... Acute toxicity and histological damage derived from exposure of Danio rerio (zebrafish) to several ionic liquids have been evaluated. ...
DOI:
10.1039/b511554j

64. Altered gene expression: A mechanism for reproductive toxicity in zebrafish exposed to benzo[a]pyrene

Author:
Hoffmann, J.L.; Oris, J.T.
Source:
Aquatic toxicology 2006 v.78 no.4 pp. 332-340
ISSN:
0166-445X
Subject:
messenger RNA; fish eggs; gonadotropins; biomarkers; animal reproduction; polymerase chain reaction; transcription (genetics); cytochrome P-450; gene expression; reproductive toxicology; Danio rerio; benzopyrenes
Abstract:
... Exposure of fish to polycyclic aromatic hydrocarbons (PAHs) has consistently been shown to have a negative impact on reproduction (e.g. decreased spawning success, decreased ovarian somatic index (OSI) and lower circulating levels of 17β-estradiol and vitellogenin). While an understanding of the mechanism behind these changes has yet to be fully elucidated, it has been proposed that PAHs can alter ...
DOI:
10.1016/j.aquatox.2006.04.007

65. Cadmium accumulation in zebrafish (Danio rerio) eggs is modulated by dissolved organic matter (DOM)

Author:
Burnison, B.K.; Meinelt, T.; Playle, R.; Pietrock, M.; Wienke, A.; Steinberg, C.E.W.
Source:
Aquatic toxicology 2006 v.79 no.2 pp. 185-191
ISSN:
0166-445X
Subject:
Danio rerio; fish eggs; water pollution; cadmium; bioaccumulation; dissolved organic matter
Abstract:
... Experiments were conducted to investigate factors influencing the accumulation of cadmium (Cd2+) into zebrafish (Danio rerio) eggs. The accumulation of 109Cd was affected by: (1) concentration, (2) time, (3) presence of dissolved organic material (DOM), (4) different origin of DOM and (5) different parts of fish eggs. Over a 5-h exposure, zebrafish eggs showed a steady increase in Cd-accumulation. ...
DOI:
10.1016/j.aquatox.2006.06.010

66. Caspase-3 Induced Apoptosis in Transgenic Zebrafish

Author:
Chiranjib Chakraborty; Gargi Saha; Biplab Sarkar; S. Pal; T. K. Chatterjee; A. K. Sadhu
Source:
Biotechnology letters 2006 v.28 no.3 pp. 189-196
ISSN:
0141-5492
Subject:
Danio rerio; actin; adults; apoptosis; caspase-3; chimerism; gene overexpression; genetically modified organisms; green fluorescent protein; kidneys; mice; models; testes; thymus gland; transgenes
Abstract:
... Zebrafish is an attractive model organism for studying apoptosis development because of its genetic accessibility. Here we describe the induction of clonally derived apoptosis in transgenic zebrafish expressing mouse caspase-3 (CASP3) under control of the zebrafish β-actin promoter (βp). Visualization of apoptotic cells, expressing a chimeric transgene encoding CASP3 fused to green fluorescent pro ...
DOI:
10.1007/s10529-005-5334-5
PubMed:
16489497

67. Cloning and characterization of tryptophan 2,3-dioxygenase gene of Zhikong scallop Chlamys farreri (Jones and Preston 1904)

Author:
Hu, Xiaoli; Bao, Zhenmin; Hu, Jingjie; Shao, Mingyu; Zhang, Lingling; Bi, Ke; Zhan, Aibin; Huang, Xiaoting
Source:
Aquaculture research 2006 v.37 no.12 pp. 1187-1194
ISSN:
1355-557X
Subject:
Caenorhabditis elegans; Chlamys; Danio rerio; Drosophila melanogaster; Mus musculus; Vibrio anguillarum; amino acid sequences; amino acids; cytoplasm; genes; gills; humans; immunohistochemistry; isoelectric point; kidneys; messenger RNA; molecular cloning; molecular weight; muscles; nucleotides; scallops; sequence homology; testes; tryptophan 2,3-dioxygenase
Abstract:
... A Zhikong scallop (Chlamys farreri Jones and Preston 1904) tryptophan 2,3-dioxygenase (TDO) gene fragment, down-regulated by Vibrio anguillarum challenge, was isolated using mRNA differential display in our previous work. In this paper, the full-length TDO gene was cloned by 5'-RACE. Chlamys farreri TDO gene consists of 1292 nucleotides encoding an expected polypeptide of 383 amino acids with an e ...
DOI:
10.1111/j.1365-2109.2006.01546.x

68. Comparative analysis of her genes during fish somitogenesis suggests a mouse/chick-like mode of oscillation in medaka

Author:
Martin Gajewski; Harun Elmasri; Manuel Girschick; Dirk Sieger; Christoph Winkler
Source:
Development genes and evolution 2006 v.216 no.6 pp. 315-332
ISSN:
0949-944X
Subject:
Danio rerio; Oryzias latipes; Tetraodontidae; chickens; essential genes; fish; mice
Abstract:
... Somitogenesis is the key developmental step, which divides the vertebrate body axis into segmentally repeated structures. It requires an intricate process of pre-patterning, which is driven by an oscillator mechanism consisting of the Delta–Notch pathway and various hairy- and Enhancer of split-related (her) genes. The subset of her genes, which are necessary to set up the segmentation clock, reve ...
DOI:
10.1007/s00427-006-0059-6

69. Comparative embryotoxicity and proteotoxicity of three carrier solvents to zebrafish (Danio rerio) embryos

Author:
Hallare, A.; Nagel, K.; Köhler, H.R.; Triebskorn, R.
Source:
Ecotoxicology and environmental safety 2006 v.63 no.3 pp. 378-388
ISSN:
0147-6513
Subject:
Danio rerio; acetone; biomarkers; developmental stages; dimethyl sulfoxide; edema; embryotoxicity; ethanol; eyes; hatching; heart rate; heat shock proteins; pigmentation; solvents; survival rate; tail
Abstract:
... The present study examines the effects of ethanol (ETOH), dimethyl sulfoxide (DMSO), and acetone on zebrafish embryos and the implications of the observed results on the use of these solvents to zebrafish early life stage tests. The embryos were exposed to different concentrations (0.0, 0.0001, 0.001, 0.01, 0.1, 0.05, 1, 1.5, and 2.0% v/v) of the respective solvents by diluting reagent-grade solve ...
DOI:
10.1016/j.ecoenv.2005.07.006
PubMed:
16125774

70. Comparative in vitro metabolism of benzo[a]pyrene by recombinant zebrafish CYP1A and liver microsomes from β-naphthoflavone-treated rainbow trout

Author:
Miranda, C.L.; Chung, W.G.; Wang-Buhler, J.L.; Musafia-Jeknic, T.; Baird, W.M.; Buhler, D.R.
Source:
Aquatic toxicology 2006 v.80 no.2 pp. 101-108
ISSN:
0166-445X
Subject:
DNA adducts; in vitro culture; liver microsomes; flavones; kinetics; Oncorhynchus mykiss; cytochrome P-450; DNA-binding proteins; oxidation; biotransformation; Danio rerio; benzopyrenes; metabolites
Abstract:
... The zebrafish (Danio rerio) is a sensitive non-mammalian model used for studying polycyclic aromatic hydrocarbon (PAH)-induced chemical carcinogenesis. The susceptibility of zebrafish to PAH-induced carcinogenesis may be related to the ability of the zebrafish P450s to bioactivate these procarcinogens. As a part of our overall effort to identify the various P450 enzymes that are involved in the ac ...
DOI:
10.1016/j.aquatox.2006.07.018

71. Conservation of RET Regulatory Function from Human to Zebrafish Without Sequence Similarity

Author:
Fisher, Shannon; Grice, Elizabeth A.; Vinton, Ryan M.; Bessling, Seneca L.; McCallion, Andrew S.
Source:
Science 2006 v.312 no.5771 pp. 276-279
ISSN:
0036-8075
Subject:
Danio rerio; gene expression; genetically modified organisms; germplasm conservation; humans; loci; regulatory sequences; reporter genes; sequence homology
Abstract:
... Evolutionary sequence conservation is an accepted criterion to identify noncoding regulatory sequences. We have used a transposon-based transgenic assay in zebrafish to evaluate noncoding sequences at the zebrafish ret locus, conserved among teleosts, and at the human RET locus, conserved among mammals. Most teleost sequences directed ret-specific reporter gene expression, with many displaying ove ...
DOI:
10.1126/science.1124070

72. DBTSS: DataBase of Human Transcription Start Sites, progress report 2006

Author:
Yamashita, Riu; Suzuki, Yutaka; Wakaguri, Hiroyuki; Tsuritani, Katsuki; Nakai, Kenta; Sugano, Sumio
Source:
Nucleic acids research 2006 v.34 pp. D86
ISSN:
0305-1048
Subject:
Danio rerio; Rhodophyta; clones; databases; genes; humans; malaria; mice; models; nucleic acids; sequence analysis
Abstract:
... DBTSS was first constructed in 2002 based on precise, experimentally determined 5' end clones. Several major updates and additions have been made since the last report. First, the number of human clones has drastically increased, going from 190 964 to 1 359 000. Second, information about potential alternative promoters is presented because the number of 5' end clones is now sufficient to determine ...

73. Developmental morphology of the cyprinid fish Inlecypris auropurpureus

Author:
Tetsuya Sado; Seishi Kimura
Source:
Ichthyological research 2006 v.53 no.1 pp. 34-40
ISSN:
1341-8998
Subject:
Chela; Danio rerio; Devario; body length; cement; eggs; eyes; fins; fish; hatching; head; juveniles; larvae; oils; yolk sac
Abstract:
... Embryonic, larval, and juvenile development of a Myanmarese cyprinid fish, Inlecypris auropurpureus, is described from laboratory-reared specimens. The eggs, measuring 0.9–1.0 mm in diameter, were demersal, almost spherical in shape, transparent and unpigmented, with a pale yellow yolk without oil globules. Hatching occurred 49–56 h after fertilization at 26.2°–27.3°C. The newly hatched larvae, me ...
DOI:
10.1007/s10228-005-0311-5

74. Differentiation of Mouse P19 Embryonic Carcinoma Stem Cells Injected into an Empty Zebrafish Egg Chorion in a Microfluidic Device

Author:
Jin-Woo LEE; Dae-Seok NA; Ji-Yoon KANG; Sang-Ho LEE; Byeong-Kwon JU
Source:
Bioscience, biotechnology, and biochemistry 2006 v.70 no.6 pp. 1325-1330
ISSN:
1347-6947
Subject:
Danio rerio; biochemistry; biocompatible materials; biotechnology; carcinoma; cardiomyocytes; cell differentiation; chorion; eggs; mice; zona pellucida
Abstract:
... Mouse P19 embryonic carcinoma (EC) stem cells were xenotransplanted into the emptied chorion, the transparent envelope of a fertilized zebrafish egg (rather than mouse native zona pellucida) combined with a microfluidic device to study P19 EC cell differentiation in the chorion biomaterial. A distilled-water jet was used to remove the innate yolk and perivitelline inner mass from the chorion. P19 ...
DOI:
10.1271/bbb.50609

75. Effects of binary mixtures of xenoestrogens on gonadal development and reproduction in zebrafish

Author:
Lin, L.L.; Janz, D.M.
Source:
Aquatic toxicology 2006 v.80 no.4 pp. 382-395
ISSN:
0166-445X
Subject:
Danio rerio; xenoestrogens; endocrine-disrupting chemicals; vitellogenin; gametogenesis; hormone agonists; estradiol; sex ratio; heat shock proteins; protein synthesis; animal reproduction; histology
Abstract:
... Previous studies exposing fish to xenoestrogens have demonstrated vitellogenin (VTG) induction, delayed gametogenesis, altered sex ratio, and decreased reproductive performance, with a majority of those studies focusing on exposure to single chemicals. The objective of this study was to determine the effects of binary mixtures of a weak estrogen receptor agonist, nonylphenol (NP) and a potent estr ...
DOI:
10.1016/j.aquatox.2006.10.004

76. The Embryonic Vertebrate Heart Tube Is a Dynamic Suction Pump

Author:
Forouhar, Arian S.; Liebling, Michael; Hickerson, Anna; Nasiraei-Moghaddam, Abbas; Tsai, Huai-Jen; Hove, Jay R.; Fraser, Scott E.; Dickinson, Mary E.; Gharib, Morteza
Source:
Science 2006 v.312 no.5774 pp. 751-753
ISSN:
0036-8075
Subject:
Danio rerio; blood; confocal laser scanning microscopy; heart; peristalsis; vertebrates
Abstract:
... The embryonic vertebrate heart begins pumping blood long before the development of discernable chambers and valves. At these early stages, the heart tube has been described as a peristaltic pump. Recent advances in confocal laser scanning microscopy and four-dimensional visualization have warranted another look at early cardiac structure and function. We examined the movement of cells in the embry ...
DOI:
10.1126/science.1123775

77. Enhanced Vitellogenin Induction of Secondary Effluents by Chlorination

Author:
An, L.; Hu, J.; Yang, M.; Jin, F.; Du, Q.; Ke, Z.
Source:
Bulletin of environmental contamination and toxicology 2006 v.77 no.1 pp. 67-73
ISSN:
0007-4861
Subject:
water pollution; water treatment; polycyclic aromatic hydrocarbons; sewage effluent; Oryzias latipes; Danio rerio; fish; early development; China
DOI:
10.1007/s00128-006-1033-8
PubMed:
16832757

78. Enhancing the tolerance of zebrafish (Danio rerio) to heavy metal toxicity by the expression of plant phytochelatin synthase

Author:
Konishi, T.; Matsumoto, S.; Tsuruwaka, Y.; Shiraki, K.; Hirata, K.; Tamaru, Y.; Takagi, M.
Source:
Journal of biotechnology 2006 v.122 no.3 pp. 316-325
ISSN:
0168-1656
Subject:
Danio rerio; transgenic animals; metal tolerance; cadmium; messenger RNA; enzymes; Arabidopsis thaliana; embryo (animal); phytochelatins; metabolic detoxification; complementary DNA; genetic transformation
DOI:
10.1016/j.jbiotec.2005.12.013

79. Expression of two vitellogenin genes (vg1 and vg3) in fathead minnow (Pimephales promelas) liver in response to exposure to steroidal estrogens and androgens

Author:
Miracle, A.; Ankley, G.; Lattier, D.
Source:
Ecotoxicology and environmental safety 2006 v.63 no.3 pp. 337-342
ISSN:
0147-6513
Subject:
Danio rerio; Pimephales promelas; androgens; dose response; estrogens; genes; liver; vitellogenin
Abstract:
... In this study, we describe the sequence for the fathead minnow (Pimephales promelas) vitellogenin 3 gene (vg3) and compare the responses of vg1 and vg3 following exposure to steroidal estrogens and androgens. The fathead minnow vg3 sequence is the second nucleotide sequence described in teleosts, following the original description of this isoform in zebrafish (Danio rerio). Following a brief water ...
DOI:
10.1016/j.ecoenv.2005.12.002
PubMed:
16464498

80. FootPrinter3: phylogenetic footprinting in partially alignable sequences

Author:
Fang, Fei; Blanchette, Mathieu
Source:
Nucleic acids research 2006 v.34 no.suppl_2 pp. W617
ISSN:
0305-1048
Subject:
Danio rerio; Internet; algorithms; binding sites; chickens; genome; mammals; nucleic acids; phylogeny; prediction; sequence alignment; transcription (genetics); transcription factors
Abstract:
... FootPrinter3 is a web server for predicting transcription factor binding sites by using phylogenetic footprinting. Until now, phylogenetic footprinting approaches have been based either on multiple alignment analysis (e.g. PhyloVista, PhastCons), or on motif-discovery algorithms (e.g. FootPrinter2). FootPrinter3 integrates these two approaches, making use of local multiple sequence alignment block ...

81. Glycomic survey mapping of zebrafish identifies unique sialylation pattern

Author:
Guérardel, Yann; Chang, Lan-Yi; Maes, Emmanuel; Huang, Chang-Jen; Khoo, Kay-Hooi
Source:
Glycobiology 2006 v.16 no.3 pp. 244-257
ISSN:
0959-6658
Subject:
Danio rerio; derivatization; developmental stages; eggs; embryo (animal); embryogenesis; enzyme activity; genetic engineering; glycoconjugates; glycolipids; glycomics; glycosylation; glycosyltransferases; nuclear magnetic resonance spectroscopy; proteomics; surveys; tandem mass spectrometry
Abstract:
... Functional genomics and proteomics studies of the developmental glycobiology of zebrafish are greatly hampered by the current lack of knowledge on its glycosylation profile. To furnish the requisite structural basis for a more insightful functional delineation and genetic manipulation, we have initiated a survey mapping of the possible expression of stage-specific glycoconjugates in zebrafish. Hig ...
DOI:
10.1093/glycob/cwj062

82. Hepatic gene expression profiling using Genechips in zebrafish exposed to 17α-ethynylestradiol

Author:
Hoffmann, J.L.; Torontali, S.P.; Thomason, R.G.; Lee, D.M.; Brill, J.L.; Price, B.B.; Carr, G.J.; Versteeg, D.J.
Source:
Aquatic toxicology 2006 v.79 no.3 pp. 233-246
ISSN:
0166-445X
Subject:
Danio rerio; animal models; water pollution; estradiol; endocrine-disrupting chemicals; genotoxicity; liver; gene expression; vitellogenin; testosterone; biomarkers; microarray technology; reverse transcriptase polymerase chain reaction
Abstract:
... Genomic, proteomic, and metabolomic technologies continue to receive increasing interest from environmental toxicologists. This interest is due to the great potential of these technologies to identify detailed modes of action and to provide assistance in the evaluation of a contaminant's risk to aquatic organisms. Our experimental model is the zebrafish (Danio rerio) exposed to reference endocrine ...
DOI:
10.1016/j.aquatox.2006.06.009

83. Hepatocyte nuclear structure and subcellular distribution of copper in zebrafish Brachydanio rerio and roach Rutilus rutilus (Teleostei, Cyprinidae) exposed to copper sulphate

Author:
Paris-Palacios, S.; Biagianti-Risbourg, S.
Source:
Aquatic toxicology 2006 v.77 no.3 pp. 306-313
ISSN:
0166-445X
Subject:
water pollution; hepatocytes; liver; bioaccumulation; copper; Rutilus rutilus; heavy metals; histology; freshwater fish; cell nucleus; Danio rerio; ultrastructure
Abstract:
... Copper is a trace element essential to life, but also a heavy metal with toxic effect clearly demonstrated. Cu induced perturbations in fish liver are well documented but the variability of the reported results is large. In this study two cyprinids, zebrafish and roach, were exposed to copper. Reported histocytological changes are either adaptative or degenerative depending on fish species, concen ...
DOI:
10.1016/j.aquatox.2006.01.001

84. Impairment of lower jaw growth in developing zebrafish exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and reduced hedgehog expression

Author:
Teraoka, H.; Dong, W.; Okuhara, Y.; Iwasa, H.; Shindo, A.; Hill, A.J.; Kawakami, A.; Hiraga, T.
Source:
Aquatic toxicology 2006 v.78 no.2 pp. 103-113
ISSN:
0166-445X
Subject:
tetrachlorodibenzo-p-dioxin; water pollution; Danio rerio; jaws; growth and development; animal morphology; embryotoxicity; genotoxicity; gene targeting; gene expression regulation; transcription factors; apoptosis; cell growth; signal transduction
Abstract:
... 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to cause a multitude of detrimental effects to developing zebrafish (Danio rerio). Previously, we demonstrated that jaw growth was impaired by TCDD exposure, but the exact mechanism underlying these malformations remained unknown. In the present study, we investigated the involvement of hedgehog genes and their downstream signaling in TCDD- ...
DOI:
10.1016/j.aquatox.2006.02.009

85. In vivo and in vitro Ah-receptor activation by commercial and fractionated pentabromodiphenylether using zebrafish (Danio rerio) and the DR-CALUX assay

Author:
Kuiper, R.V.; Murk, A.J.; Leonards, P.E.G.; Grinwis, G.C.M.; Berg, M. van den; Vos, J.G.
Source:
Aquatic toxicology 2006 v.79 no.4 pp. 366-375
ISSN:
0166-445X
Subject:
in vivo studies; flame retardants; polycyclic aromatic hydrocarbons; immunohistochemistry; gene activation; bioassays; ethers; toxicity testing; animal models; immunotoxicity; in vitro studies; gene induction; Danio rerio; histopathology
Abstract:
... The present study addresses the toxicity of a commercial pentabrominated diphenylether (PeBDE) flame retardant mixture, DE-71, in a model aquatic vertebrate. Four weeks' exposure of juvenile zebrafish (Danio rerio) to water-borne DE-71 resulted in dose-dependent induction of CYP1A immunoreactivity, predominantly in the endocardium and the endothelium of larger blood vessels, such as ventral aorta ...
DOI:
10.1016/j.aquatox.2006.07.005

86. Male-Biased Sex Ratios and Vitellogenin Induction in Zebrafish Exposed to Effluent Water from a Swedish Pulp Mill

Author:
Örn, Stefan; Svenson, Anders; Viktor, Tomas; Holbech, Henrik; Norrgren, Leif
Source:
Archives of environmental contamination and toxicology 2006 v.51 no.3 pp. 445-451
ISSN:
0090-4341
Subject:
Danio rerio; androgens; estrogenic properties; fish; image analysis; males; pulp; pulp and paper mill effluents; sex ratio; testes; vitellogenin; yeasts
Abstract:
... Juvenile zebrafish (Danio rerio) were exposed to different dilutions (0, 0.67, 2.5, 10, and 50%) of effluent water from a Swedish pulp mill that previously has been reported to be androgenic to fish. Exposure was performed between days 10-38 days post-hatch. Fish were sampled for whole-body vitellogenin concentrations at day 38 post-hatch and for histological examination of gonads at day 60 post-h ...
DOI:
10.1007/s00244-005-0199-0
PubMed:
16823521

87. Mannose 6-phosphate receptors in an ancient vertebrate, zebrafish

Author:
Nolan, Catherine M.; McCarthy, Karena; Eivers, Edward; Jirtle, Randy L.; Byrnes, Lucy
Source:
Development genes and evolution 2006 v.216 no.3 pp. 144-151
ISSN:
0949-944X
Subject:
Danio rerio; binding sites; embryogenesis; genes; hydrolases; in vivo studies; mannose; models; neurodevelopment; receptors
Abstract:
... The endosome/lysosome system plays key roles in embryonic development, but difficulties posed by inaccessible mammalian embryos have hampered detailed studies. The accessible, transparent embryos of Danio rerio, together with the genetic and experimental approaches possible with this organism, provide many advantages over rodents. In mammals, mannose 6-phosphate receptors (MPRs) target acid hydrol ...
DOI:
10.1007/s00427-005-0046-3
PubMed:
16411117

88. Molecular Identification, Polymorphism, and Expression Analysis of Major Histocompatibility Complex Class IIA and B Genes of Turbot (Scophthalmus maximus)

Author:
Yu-Xi Zhang; Song-Lin Chen
Source:
Marine biotechnology 2006 v.8 no.6 pp. 611-623
ISSN:
1436-2228
Subject:
Cichlidae; Danio rerio; Ginglymostoma cirratum; Oncorhynchus mykiss; Pagrus major; Paralichthys olivaceus; Salmo salar; Scophthalmus maximus; T-lymphocytes; Vibrio anguillarum; adaptive immunity; alleles; amino acid sequences; bacteria; bass; carp; complementary DNA; flounder; humans; kidneys; liver; loci; major histocompatibility complex; messenger RNA; mice; nucleotide sequences; peptides; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; spleen; tissues; turbot
Abstract:
... Major histocompatibility complex (MHC) class II has a central role in the adaptive immune system by presenting foreign peptides to the T-cell receptor. The full lengths of MHC class II A and B cDNA were cloned from turbot by homology cloning and rapid amplification of cDNA ends polymerase chain reaction (RACE PCR), and genomic organization, molecular polymorphism, and expression of turbot class II ...
DOI:
10.1007/s10126-005-6174-y

89. Photodecomposition of bisphenol A on nanometer-sized TiO2 thin film and the associated biological toxicity to zebrafish (Danio rerio) during and after photocatalysis

Author:
Yeo, M.K.; Kang, M.
Source:
Water research 2006 v.40 no.9 pp. 1906-1914
ISSN:
0043-1354
Subject:
wastewater treatment; photolysis; phenols; titanium dioxide; toxicity testing; Danio rerio; fish; mortality; morphogenesis
DOI:
10.1016/j.watres.2005.12.034
PubMed:
16620905

90. Rearing zebrafish (Danio rerio) larvae without live food: evaluation of a commercial, a practical and a purified starter diet on larval performance

Author:
Carvalho, António Paulo; Araújo, Leonor; Santos, Miguel M.
Source:
Aquaculture research 2006 v.37 no.11 pp. 1107-1111
ISSN:
1355-557X
Subject:
Danio rerio; artificial diets; experimental diets; labor; models; nauplii; rearing; starter diets; survival rate; traditional foods
Abstract:
... Owing to the increasing importance of zebrafish as a vertebrate model in many fields of research, efforts must be made to breed and maintain this species in laboratory. Zebrafish larvae are traditionally reared on cultured live paramecia during the first 9 days of exogenous feeding, followed by a combination of paramecia and artemia nauplii until day 21, making larval rearing expensive, labour int ...
DOI:
10.1111/j.1365-2109.2006.01534.x

91. Sex steroids in the female zebrafish (Danio rerio): Effects of cyproterone acetate and leachate-contaminated sediment extract

Author:
Linderoth, M.; Ledesma, M.; Zebuhr, Y.; Balk, L.
Source:
Aquatic toxicology 2006 v.79 no.2 pp. 192-200
ISSN:
0166-445X
Subject:
Danio rerio; perch; females; lakes; sediment contamination; acetates; leachates; endocrine-disrupting chemicals; steroids; estradiol; testosterone; spawning; animal reproduction; biomarkers; toxicity testing; Sweden
Abstract:
... In Molnbyggen, a leachate-contaminated lake in Sweden, effects on the reproductive system of perch included a decreased frequency of sexually mature female perch, reduced gonadosomatic index and decreased plasma levels of androstenedione and testosterone, but the contaminants responsible for these effects have not yet been identified. A biomarker-directed fractionation approach could be used to na ...
DOI:
10.1016/j.aquatox.2006.06.011

92. Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme

Author:
Uemura, Satoshi; Kurose, Takahiro; Suzuki, Tomoko; Yoshida, Sayaka; Ito, Makoto; Saito, Masaki; Horiuchi, Masataka; Inagaki, Fuyuhiko; Igarashi, Yasuyuki; Inokuchi, Jin-ichi
Source:
Glycobiology 2006 v.16 no.3 pp. 258-270
ISSN:
0959-6658
Subject:
Danio rerio; Escherichia coli; asparagine; complementary DNA; enzyme activity; glutamine; glycosylation; glycosyltransferases; humans; mice; models; mutants; sialic acid
Abstract:
... The sialyltranferase ST3Gal-V transfers a sialic acid to lactosylceramide. We investigated the role of each of the N-glycans modifying mouse ST3Gal-V (mST3Gal-V) by measuring the in vitro enzyme activity of Chinese hamster ovary (CHO) cells transfected with ST3Gal-V cDNA or its mutants. By examining mutants of mST3Gal-V, in which each asparagine was replaced with glutamine (N180Q, N224Q, N334Q), w ...
DOI:
10.1093/glycob/cwj060

93. Transgenic Zebrafish Expressing Chicken Lysozyme Show Resistance against Bacterial Diseases

Author:
Yazawa, R.; Hirono, I.; Aoki, T.
Source:
Transgenic research 2006 v.15 no.3 pp. 385-391
ISSN:
0962-8819
Subject:
Danio rerio; transgenic animals; genetic transformation; injection; chickens; egg albumen; lysozyme; genes; recombinant DNA; promoter regions; keratin; Paralichthys olivaceus; gene expression; messenger RNA; disease resistance; Flavobacterium columnare; Edwardsiella tarda; bacterial infections; fish diseases; animal pathogenic bacteria
DOI:
10.1007/s11248-006-0009-0
PubMed:
16779653

94. Use of PCR-restriction fragment length polymorphism for the identification of zoonotic mycobacteriosis in zebrafish caused by Mycobacterium abscessus and Mycobacterium chelonae

Author:
Seok, S.H.; Koo, H.C.; Kasuga, A.; Kim, Y.; Lee, E.G.; Lee, H.; Park, J.H.; Baek, M.W.; Lee, H.Y.; Kim, D.J.
Source:
Veterinary microbiology 2006 v.114 no.3-4 pp. 292-297
ISSN:
0378-1135
Subject:
restriction fragment length polymorphism; occupational health and safety; Mycobacterium abscessus; lesions (animal); zoonoses; polymerase chain reaction; laboratory animals; emerging diseases; disease outbreaks; pathogen identification; fish diseases; mycobacterial diseases; fish; Mycobacterium chelonae; molecular epidemiology; Danio rerio; microbial genetics; disease diagnosis; South Korea
Abstract:
... Skin ulcers, scoliosis, and dropsy-like scale edema were observed in laboratory-maintained zebrafish. Affected fish had multifocal granulomas not only in internal organs such as the liver, intestine, genital organs, kidney, muscle, and spleen but also in the fin, epithelium, gills, and sclera of the eyes. Large numbers of acid-fast-rod-shaped bacteria were observed within the necrotic centers of w ...
DOI:
10.1016/j.vetmic.2005.12.006

95. Whole-body cortisol is an indicator of crowding stress in adult zebrafish, Danio rerio

Author:
Ramsay, J.M.; Feist, G.W.; Varga, Z.M.; Westerfield, M.; Kent, M.L.; Schreck, C.B.
Source:
Aquaculture 2006 v.258 no.1-4 pp. 565-574
ISSN:
0044-8486
Subject:
Danio rerio; physiological response; blood plasma; cortisol; body weight; animal growth; stocking rate; fish feeding; fish culture
Abstract:
... Plasma cortisol levels have been used to evaluate the stress response in a variety of cultured fish species. However, little is known about the stress response of zebrafish, Danio rerio, despite its extensive use as a laboratory research organism. Due to its small size, evaluation of whole-body cortisol has provided a means to assess zebrafish stress levels Understanding the role of crowding on ze ...
DOI:
10.1016/j.aquaculture.2006.04.020

96. The Zebrafish Information Network: the zebrafish model organism database

Author:
Sprague, Judy; Bayraktaroglu, Leyla; Clements, Dave; Conlin, Tom; Fashena, David; Frazer, Ken; Haendel, Melissa; Howe, Douglas G.; Mani, Prita; Ramachandran, Sridhar; Schaper, Kevin; Segerdell, Erik; Song, Peiran; Sprunger, Brock; Taylor, Sierra; Van Slyke, Ceri E.; Westerfield, Monte
Source:
Nucleic acids research 2006 v.34 pp. D581
ISSN:
0305-1048
Subject:
Danio rerio; databases; genes; genetic markers; models; mutants; nucleic acids; nucleotide sequences
Abstract:
... The Zebrafish Information Network (ZFIN; http://zfin.org) is a web based community resource that implements the curation of zebrafish genetic, genomic and developmental data. ZFIN provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information. Recent enhancements to ZFIN include (i ...

97. Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs

Author:
Giraldez, Antonio J.; Mishima, Yuichiro; Rihel, Jason; Grocock, Russell J.; Van Dongen, Stijn; Inoue, Kunio; Enright, Anton J.; Schier, Alexander F.
Source:
Science 2006 v.312 no.5770 pp. 75-79
ISSN:
0036-8075
Subject:
Danio rerio; early development; embryogenesis; genes; mechanism of action; messenger RNA; microRNA; microarray technology; morphogenesis; vertebrates
Abstract:
... MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA ...
DOI:
10.1126/science.1122689

98. Zebrafish and medaka as models for bone research including implications regarding space-related issues

Author:
Renn, J.; Winkler, C.; Schartl, M.; Fischer, R.; Goerlich, R.
Source:
Protoplasma 2006 v.229 no.2-4 pp. 209-214
ISSN:
0033-183X
Subject:
Danio rerio; amino acid sequences; bone metabolism; bones; cartilage; fish; gravity; homeostasis; models; regulator genes
Abstract:
... Teleost fish develop bones directly from mesenchymal condensations and from cartilage precursors. At the cellular level, the involved cell populations share many features with their mammalian counterparts. In addition, several genes are already described in fish showing high homology in amino acid sequence and expression with the corresponding genes of tetrapods that are involved in bone metabolis ...
DOI:
10.1007/s00709-006-0215-x

99. The early vertebrate Danio rerio Mr 46000 mannose-6-phosphate receptor: biochemical and functional characterisation

Author:
Koduru, Suresh; Vegiraju, Suryanarayana Raju; Nadimpalli, Siva Kumar; von Figura, Kurt; Pohlmann, Regina; Dennes, André
Source:
Development genes and evolution 2006 v.216 no.3 pp. 133-143
ISSN:
0949-944X
Subject:
Danio rerio; amino acid sequences; arginine; cysteine; endocytosis; enzymes; fish; lysosomes; molluscs; proline; receptors; serine; tail
Abstract:
... Mannose-6-phosphate receptors (MPRs) have been identified in a wide range of species from humans to invertebrates such as molluscs. A characteristic of all MPRs is their common property to recognize mannose-6-phosphate residues that are labelling lysosomal enzymes and to mediate their targeting to lysosomes in mammalian cells by the corresponding receptor proteins. We present here the analysis of ...
DOI:
10.1007/s00427-005-0043-6
PubMed:
16362416

100. The effects of dietary iron concentration on gastrointestinal and branchial assimilation of both iron and cadmium in zebrafish (Danio rerio)

Author:
Cooper, C.A.; Handy, R.D.; Bury, N.R.
Source:
Aquatic toxicology 2006 v.79 no.2 pp. 167-175
ISSN:
0166-445X
Subject:
Danio rerio; fish feeding; iron; cadmium; dietary exposure; toxicity; gastrointestinal system; gills; uptake mechanisms; bioaccumulation; DNA primers; transcription (genetics)
Abstract:
... Zebrafish (Danio rerio) were fed either a diet containing 33 mg Fe kg-1 (low) or 95 mg Fe kg-1 (normal) for 10 weeks, after which short-term Cd and Fe uptake by the gastrointestinal tract and gill was assessed. Carcass metal content and transcript levels of the iron importer, Divalent Metal Transporter 1 (DMT1) and an iron exporter, ferroportin1, in both the gastrointestinal tract and gill were al ...
DOI:
10.1016/j.aquatox.2006.06.008