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... The ability to design agonists that target peptide signaling is a strategy to delineate underlying mechanisms and influence biology. A sequence that uniquely characterizes a peptide provides a distinct site to generate novel agonists. Drosophila melanogaster sulfakinin encodes non-sulfated drosulfakinin I (nsDSK I; FDDYGHMRF-NH2) and nsDSK II (GGDDQFDDYGHMRF-NH2). Drosulfakinin is typical of sulfa ...
... Declines in mitochondrial functions are associated with aging. The combination of 5‐aminolevulinic acid (5‐ALA) and sodium ferrous citrate (SFC) improves mitochondrial functions in cultured cells. In this study, we investigated the effects of dietary supplementation with 5‐ALA and SFC (5‐ALA/SFC) on the healthspan and life span of Drosophila melanogaster. Adult Drosophila fruit flies were fed corn ...
... When a new student first begins to push flies, an immediate skill that must be learned is sorting the sexes. In Drosophila melanogaster several sexually dimorphic characters can be used to readily distinguish males from females including abdominal pigmentation, male sex combs and genital morphology. Another, often-overlooked, sexual dimorphism is adult abdominal segment number. Externally, adult D ...
... Ecotherms adjust their physiology to environmental temperatures. Long‐term exposures to heat or cold typically induce acclimation responses that generate directional, but reversible shifts in thermal tolerance and performance. However, less is known about how short exposure in different life stages will affect the adult phenotype. In the present study, we compared the effects of long‐term temperat ...
Elizabeth K. Peterson; Diane T. Wilson; Bernard Possidente; Phillip McDaniel; Eric J. Morley; Debra Possidente; Kurt T. Hollocher; Douglas M. Ruden; Helmut V.B. Hirsch
... We examined accumulation, sequestration, elimination, and genetic variation for lead (Pb) loads within and between generations of Drosophila melanogaster. Flies were reared in control or leaded medium at various doses and tested for their Pb loads at different stages of development (larvae, eclosion, newly-eclosed adults, and mature adults). Pb loads were tested using Inductively Coupled Plasma Ma ...
... Similar to several environmental monitoring studies, the present study used Drosophila melanogaster as a model nontarget organism to explore the interfering effects of an organophosphate (OP) insecticide acephate on insect life cycle parameters. Acephate, a common OP, is readily available in nature from agricultural sources as an environmental contaminant. Along with target pests, nontarget fruit ...
Acetobacter tropicalis; Anopheles stephensi; Bactrocera oleae; DNA libraries; Drosophila melanogaster; Malpighian tubules; acetic acid bacteria; adults; digestive system; green fluorescent protein; insect models; insects; larvae; pests; polymerase chain reaction; ribosomal RNA; symbionts; Greece; United States
Abstract:
... Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with Bactrocera oleae, one of the major agricultural pests in olive-producing countries. Bacterial 16S rRNA gene libraries and ultrastructural analyses revealed the presence of several bacterial taxa associated with this insect, among which Acetobacter tropicalis was predominant. The recent incre ...
Drosophila melanogaster; acute exposure; adults; body size; feeding behavior; fruit flies; hyperoxia; insect physiology; instars; larvae; long term effects; metabolism; oxygen; rearing
Abstract:
... All insects studied to date show reduced growth rates in hypoxia. Drosophila melanogaster reared in moderate hypoxia (10kPa PO2) grow more slowly and form smaller adults, but the mechanisms responsible are unclear, as metabolic rates are not oxygen-limited. It has been shown that individual fruit flies do not grow larger in hyperoxia (40kPa PO2), but populations of flies evolve larger size. Here w ...
1-propanol; Drosophila melanogaster; adults; alcohol dehydrogenase; alleles; body weight; enzyme activity; ethanol; hexanols; homozygosity; juveniles; loci
Abstract:
... The process of adaptation to hexanol-1 was studied in the Groningen population of Drosophila melanogaster Meigen (Diptera). In view of the prominent role of the alcohol dehydrogenase (Adh) locus in survival on alcohol supplemented medium the contribution of the Adh locus to adaptation was investigated. Strains, either homozygous for the Adh F or the Adh s allele, were kept on food supplemented wit ...
... Chimeric genes, which form through the genomic fusion of two protein-coding genes, are a significant source of evolutionary novelty in Drosophila melanogaster. However, the propensity of chimeric genes to produce adaptive phenotypic changes is not fully understood. Here, we describe the chimeric gene Quetzalcoatl (Qtzl; CG31864), which formed in the recent past and swept to fixation in D. melanoga ...
... The effect of acute treatment of methyl parathion (MP) on the expression of BSP/GSH-agarose purified glutathione S-transferases (GSTs) in Drosophila melanogaster was investigated. Using 2-D gel analysis of the identified Epsilon-class, only DmGSTE6 (100%) and DmGSTE7 (72%) demonstrated significant increases in expression, suggesting the possibility that both may be involved in MP metabolism. A sma ...
... Adult Drosophila melanogaster locate food resources by using distinct olfactory cues that often are associated with the fermentation of fruit. However, in addition to being an odorous food source and providing a possible site for oviposition, fermenting fruit also provides a physical substrate upon which flies can attract and court a potential mate. In this study, we demonstrate that Drosophila ad ...
... The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adults (AG) and in both stages (SG). A total of 913 genes (5.4% of the transcript co ...
... Numerous experimental life‐history studies on aging are mainly baised on two classical models—fruit fly Drosophila melanogaster (Meigen) and nematode Caenorhabditis elegans (Maupas)—with relatively little attention given to other organisms with different life‐history characters. Two‐spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) differs from many other arthropods in that the f ...
Drosophila melanogaster; adults; chemical ecology; climatic factors; data collection; genetically modified organisms
Abstract:
... The cuticle of all insects is covered with hydrocarbons which have multiple functions. Cuticular hydrocarbons (CHCs) basically serve to protect insects against environmental harm and reduce dehydration. In many species, some CHCs also act as pheromones. CHCs have been intensively studied in Drosophila species and more especially in D. melanogaster. In this species, flies produce about 40 CHCs form ...
foods; Drosophila melanogaster; adults; larvae; fungi; population density; mortality; biological development; intraspecific competition; aggregation pheromones; body size
Abstract:
... 1. Aggregation pheromones can evolve when individuals benefit from clustering. Such a situation can arise with an Allee effect, i.e. a positive relationship between individual fitness and density of conspecifics. Aggregation pheromone in Drosophila induces aggregated oviposition. The aim of the work reported here was to identify an Allee effect in the larval resource exploitation by Drosophila mel ...
Drosophila melanogaster; adults; blue light; circadian rhythm; cryptochromes; fruit flies; locomotion; mutants; rearing
Abstract:
... The fruit fly (Drosophila melanogaster) offers an extremely useful system to study circadian clock function at the behavioral, molecular, cellular, and organismal levels. We reared wild type (Oregon R⁺) and mutant flies – vestigial (vg) and cryptochrome depleted (cryᵇ) flies in 12:12 light:dark cycles (LD 12:12) from the embryo to adult stage. Later, the locomotor activity rhythms of these newly e ...
... Drosophila sechellia is a dietary specialist species of fruit fly that has evolved resistance to the toxic secondary defence compounds produced by the fruit of its preferred host plant Morinda citrifolia. The genetic basis of adult toxin resistance is the result of evolution at five loci across the genome. Genetic mapping between D. sechellia and Drosophila simulans and subsequent functional studi ...
... The impact of nutritional deficiencies early in life in determining life‐history variation in organisms is well recognized. The negative effects of inbreeding on fitness are also well known. Contrary to studies on vertebrates, studies on invertebrates are not consistent with the observation that inbreeding compromises resistance to parasites and pathogens. In this study, we investigated the effect ...
... Because of their decreased overall fitness and genetic variability inbred individuals are expected to show reduced survival and lifespan under most environmental conditions as compared with outbred individuals. Whereas evidence for the deleterious effects of inbreeding on lifespan has been previously provided, only a few studies have investigated effects of inbreeding on survival under starved con ...