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... Copper-contaminated soils were managed with aided phytoextraction in 31 field plots at a former wood preservation site, using a single incorporation of compost (OM) and dolomitic limestone (DL) followed by a crop rotation with tobacco and sunflower. Six amended plots, with increasing total soil Cu, and one unamended plot were selected together with a control uncontaminated plot. The mobility and p ...
... Foliar vascular anatomy and photosynthesis were evaluated for a number of summer annual species that either load sugars into the phloem via a symplastic route (Cucumis sativus L. cv. Straight Eight; Cucurbita pepo L. cv. Italian Zucchini Romanesco; Citrullus lanatus L. cv. Faerie Hybrid; Cucurbita pepo L. cv. Autumn Gold) or an apoplastic route (Nicotiana tabacum L.; Solanum lycopersicum L. cv. Br ...
... Plastomic replacement of the tobacco (Nicotiana tabacum) Rubisco large subunit gene (rbcL) with that from sunflower (Helianthus annuus; rbcLS) produced tobaccoRst transformants that produced a hybrid Rubisco consisting of sunflower large and tobacco small subunits (LsSt). The tobaccoRst plants required CO₂ (0.5% v/v) supplementation to grow autotrophically from seed despite the substrate saturated ...
... BACKGROUND: We have previously reported that the seed-specific overexpression of sunflower (Helianthus annuus L.) Heat Shock Factor A9 (HaHSFA9) enhanced seed longevity in transgenic tobacco (Nicotiana tabacum L.). In addition, the overexpression of HaHSFA9 in vegetative organs conferred tolerance to drastic levels of dehydration and oxidative stress. RESULTS: Here we found that the combined overe ...
... Tobacco streak virus (TSV) is economically the most important virus infecting several crop plants in India. Symptomatic samples collected from sunflower and okra fields were subjected to RT-PCR with TSV replicase gene specific primers. The replicase (Rep) genes of these isolates were sequenced in order to develop transgenic resistance to Tobacco streak virus (TSV) in tobacco (Nicotiana tabacum L.) ...
... Transgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of small heat shock protein (sHSP) gene from sunflower hp17.7 G4. The promotor was developmentally regulated during zygotic and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) bi ...
... The glutathione S‐transferase (GST) gene resists stress‐induced toxins and is widely expressed in eukaryotes. The role of a novel GST gene (HaGSTp1) in disease resistance in sunflower (Helianthus annuus L.) was investigated using the transcriptome library data of Sclerotina sclerotiorum (Lib.) de Bary‐infected sunflower cultivar ‘LSK‐2’ and cloned. The full length of HaGSTp1 is 639 bp. It encodes ...
... Branched broomrape (Orobanche ramosa L.) is a chlorophyll-lacking, root parasitic plant that infects many crops and wild species (2). Plants are densely hairy with minute, glandular hairs, particularly on flowers and upper stems. Stems are erect, often branched just above the ground, and brown to straw yellow. Leaves are sparse, triangular, dark brown or purple, and arranged alternately mainly nea ...
... Geranylgeranyl pyrophosphate synthase (GGPS) is a key enzyme for a structurally diverse class of isoprenoid biosynthetic metabolites including gibberellins, carotenoids, chlorophylls and rubber. We expressed a chloroplast‐targeted GGPS isolated from sunflower (Helianthus annuus) under control of the cauliflower mosaic virus 35S promoter in tobacco (Nicotiana tabacum). The resulting transgenic toba ...
... Most adult Lepidoptera feed on nectar, whereas caterpillars consume mainly structural tissue such as leaves, stems, flowers, and/or fruits. This may result in behavioral trade-offs in which search time for high-quality oviposition sites suitable for larval food is restricted by adult foraging needs. Here we report on the preference for and performance on flowering and nonflowering host plants of t ...
... T-phylloplanin proteins secreted to aerial surfaces of tobacco (Nicotiana tabacum) by short procumbent trichomes inhibit spore germination and blue mold disease caused by the oomycete pathogen Peronospora tabacina. Many other plants were found to contain water-washed leaf surface proteins (phylloplanins), but the functions and properties of these are not known. Here we extend earlier evidence for ...
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
... We show that seed-specific overexpression of the sunflower (Helianthus annuus) HaHSFA9 heat stress transcription factor (HSF) in tobacco (Nicotiana tabacum) enhances the accumulation of heat shock proteins (HSPs). Among these proteins were HSP101 and a subset of the small HSPs, including proteins that accumulate only during embryogenesis in the absence of thermal stress. Levels of late embryogenes ...
... Copper (Cu) locally contaminates soils and might negatively affect growth and yield of crops. A better understanding of plant copper tolerance and accumulation is needed in order to breed more Cu-tolerant or Cu-efficient crops. Cu tolerance was evaluated in different varieties of seven species (Brassica carinata, B. juncea, B. napus, Cynara cardunculus, Helianthus annuus, Nicotiana tabacum and Rap ...
... Root-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. We found that the promoter of the Hahsp17.7G4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita. In roots containing a GUS reporter fusion to the Hahsp17.7G4 pr ...
... Plant MADS box transcription factors play key roles in many developmental processes, including the transition to reproductive phase and determination of floral meristem and organs identity. Here we describe the obtaining and characterization of transgenic Nicotiana tabacum L. plants with constitutive expression of Asteraceae MADS box genes CDM111, CDM41, CDM8, CDM77, CDM44 (Chrysanthemum morifoliu ...
... Substantial lateral CO₂ diffusion rates into leaf areas where stomata were blocked by grease patches were quantified by gas exchange and chlorophyll a fluorescence imaging in different species across the full range of photosynthetic photon flux densities (PPFD). The lateral CO₂ flux rate over short distances was substantial and very similar in five dicotyledonous species with different vascular an ...