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1. Enhanced biodegradation activity towards poly(ethyl acrylate) and poly(vinyl acetate) by anchor peptide assistant targeting

Author:
Zhanzhi Liu; Guangyao Li; Fengshan Zhang; Jing Wu
Source:
Journal of biotechnology 2022 v.349 pp. 47-52
ISSN:
0168-1656
Subject:
Escherichia coli; Humicola insolens; biodegradation; biotechnology; cutinase; enzymatic treatment; flocculation; forests; peptides; polyesters; product quality; pulp; pulp and paper industry; raw materials; sediments; waste paper; wood
Abstract:
... The paper industry is one of the most important basic raw material pillar industries. With the decrease of forest wood resources, the recycling of wastepaper has drawn increasingly attention. However, the stickies generated in the process of wastepaper recycling will flocculate and deposite in the pulp, resulting in production accidents and inferior product quality. The biological enzymatic method ...
DOI:
10.1016/j.jbiotec.2022.03.003

2. Enzymatic Epoxidation of Long-Chain Terminal Alkenes by Fungal Peroxygenases

Author:
Esteban D. Babot; Carmen Aranda; Jan Kiebist; Katrin Scheibner; René Ullrich; Martin Hofrichter; Angel T. Martínez; Ana Gutiérrez
Source:
Antioxidants 2022 v.11 no.3 pp. -
ISSN:
2076-3921
Subject:
Agrocybe; Coprinopsis cinerea; Daldinia; Humicola insolens; Marasmius; alkenes; cytochrome P-450; enols; epoxidation reactions; epoxides; fungi; hydroxylation; pH; polymers
Abstract:
... Terminal alkenes are among the most attractive starting materials for the synthesis of epoxides, which are essential and versatile intermediate building blocks for the pharmaceutical, flavoring, and polymer industries. Previous research on alkene epoxidation has focused on the use of several oxidizing agents and/or different enzymes, including cytochrome P450 monooxygenases, as well as microbial w ...
DOI:
10.3390/antiox11030522

3. Novel efficient enzymatic synthesis of the key-reaction intermediate of PET depolymerization, mono(2-hydroxyethyl terephthalate) – MHET

Author:
Erika de Queiros Eugenio; Ivone Sampaio Pereira Campisano; Ayres Guimarães Dias; Aline Machado de Castro; Maria Alice Zarur Coelho; Marta Antunes Pereira Langone
Source:
Journal of biotechnology 2022 v.358 pp. 102-110
ISSN:
0168-1656
Subject:
Humicola insolens; biotechnology; calorimetry; cutinase; depolymerization; enzymatic hydrolysis; enzyme kinetics; ethylene glycol; hydrolysis; mathematical models; nuclear magnetic resonance spectroscopy; polymers; thermogravimetry
Abstract:
... Poly(ethylene terephthalate) (PET) is one of the main synthetic plastics produced worldwide. The extensive use of this polymer causes several problems due to its low degradability. In this scenario, biocatalysts dawn as an alternative to enhance PET recycling. The enzymatic hydrolysis of PET results in a mixture of terephthalic acid (TPA), ethylene glycol (EG), mono-(2-hydroxyethyl) terephthalate ...
DOI:
10.1016/j.jbiotec.2022.08.019

4. Biocatalytic depolymerization of waste polyester mooring lines from oil and gas offshore platforms made of poly(ethylene terephthalate) (PET)

Author:
Aline M de Castro; Adriano Carniel; Sonia M Menezes; Luiz S Chinelatto Júnior; Hercílio de Honorato; Aline P Lima; Erica G Chaves
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.3 pp. 709-718
ISSN:
0268-2575
Subject:
Fourier transform infrared spectroscopy; Humicola insolens; X-radiation; biotechnology; catalytic activity; circular economy; crystal structure; cutinase; depolymerization; molecular weight; nuclear magnetic resonance spectroscopy; oils; pH; pollution; temperature; thermogravimetry
Abstract:
... BACKGROUND: The global concern of plastic pollution in the environment has consistently been emerging in the past years. As one of the main polymers constituting single‐use products, poly(ethylene terephthalate) (PET) is found in a plethora of packages for diverse sectors, and its recycling has been broadly addressed. However, other relevant industrial PET wastes have not been under investigation ...
DOI:
10.1002/jctb.6955

5. CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Author:
Chao Fan; Wei Zhang; Xiaoyun Su; Wangli Ji; Huiying Luo; Yuhong Zhang; Bo Liu; Bin Yao; Huoqing Huang; Xinxin Xu
Source:
Biotechnology for biofuels 2021 v.14 no.1 pp. 206
ISSN:
1754-6834
Subject:
CRISPR-Cas systems; Hepatitis delta virus; Humicola insolens; biofuels; biotechnology; endo-1,4-beta-glucanase; fungi; gene editing; genes; hybrids; promoter regions; transcription factors
Abstract:
... BACKGROUND: Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism. RESULTS: Herein, a CRISPR/Cas9 genome-editing system was established in H. insolens based on a hybri ...
DOI:
10.1186/s13068-021-02057-y

6. Covalent binding and in-situ immobilization of lipases on a flexible nanoporous material

Author:
Saba Ghasemi; Maryam Yousefi; Ahmad Nikseresht; Hoda Omidi
Source:
Process biochemistry 2021 v.102 pp. 92-101
ISSN:
1359-5113
Subject:
Humicola insolens; ambient temperature; carboxylic ester hydrolases; nanopores; pH; thermal stability; ultrasonic treatment
Abstract:
... In this study, the flexible nanoporous MIL-53(Fe) (MIL = Materials of Institute Lavoisier) was used as an efficient support for in-situ and covalent binding immobilization of Humicola insolens lipase (HIL) and Rhizomucour miehei lipase (RML). In the covalent attachment procedure, the support synthesized under ultrasound irradiation was functionalized by N,N-dicyclohexylcarbodiimide and then attach ...
DOI:
10.1016/j.procbio.2020.12.013

7. Experimental and mathematical modeling approaches for biocatalytic post-consumer poly(ethylene terephthalate) hydrolysis

Author:
Erika de Queiros Eugenio; Ivone Sampaio Pereira Campisano; Aline Machado de Castro; Maria Alice Zarur Coelho; Marta Antunes Pereira Langone
Source:
Journal of biotechnology 2021 v.341 pp. 76-85
ISSN:
0168-1656
Subject:
Humicola insolens; biocatalysis; biocatalysts; biotechnology; cutinase; depolymerization; environmental impact; enzymatic hydrolysis; half life; hydrolysis; pH; thermal stability; value-added products; wastes
Abstract:
... The environmental impact arising from poly(ethylene terephthalate) (PET) waste is notable worldwide. Enzymatic PET hydrolysis can provide chemicals that serve as intermediates for value-added product synthesis and savings in the resources. In the present work, some reaction parameters were evaluated on the hydrolysis of post-consumer PET (PC-PET) using a cutinase from Humicola insolens (HiC). The ...
DOI:
10.1016/j.jbiotec.2021.09.007

8. Feruloylation of polysaccharides from cranberry and characterization of their prebiotic properties

Author:
Eugenio Spadoni Andreani; Mingqin Li; Jennifer Ronholm; Salwa Karboune
Source:
Food bioscience 2021 v.42 pp. 101071
ISSN:
2212-4292
Subject:
Bifidobacterium longum; Humicola insolens; Lactobacillus brevis; antioxidants; cranberries; esterification; fatty acid composition; fermentation; feruloyl esterase; inulin; pectins; pomace; prebiotics; propionic acid; raffinose; short chain fatty acids
Abstract:
... Humicola insolens feruloyl esterase was immobilized on chemically modified epoxy-activated supports of different pore sizes. Native and immobilized feruloyl esterases were investigated for their esterification activity on raffinose in surfactantless microemulsions. Pectic polysaccharides, obtained from cranberry pomace using chelating agent (CH) and diluted alkaline (DA) extractions, were then est ...
DOI:
10.1016/j.fbio.2021.101071

9. Glucomannan and beta-glucan degradation by Mytilus edulis Cel45A: Crystal structure and activity comparison with GH45 subfamily A, B and C

Author:
Laura Okmane; Gustav Nestor; Emma Jakobsson; Bingze Xu; Kiyohiko Igarashi; Mats Sandgren; Gerard J. Kleywegt; Jerry Ståhlberg
Source:
Carbohydrate polymers 2022 v.277 pp. 118771
ISSN:
0144-8617
Subject:
Humicola insolens; Mytilus edulis; Phanerochaete chrysosporium; Trichoderma reesei; barley; beta-glucans; beta-mannosidase; carboxymethylcellulose; cellobiose; cold; crystal structure; enzymatic hydrolysis; glucose; glycosides; hydrolysis; konjac mannan; nuclear magnetic resonance spectroscopy
Abstract:
... The enzymatic hydrolysis of barley beta-glucan, konjac glucomannan and carboxymethyl cellulose by a β-1,4-D-endoglucanase MeCel45A from blue mussel, Mytilus edulis, which belongs to subfamily B of glycoside hydrolase family 45 (GH45), was compared with GH45 members of subfamilies A (Humicola insolens HiCel45A), B (Trichoderma reesei TrCel45A) and C (Phanerochaete chrysosporium PcCel45A). Furthermo ...
DOI:
10.1016/j.carbpol.2021.118771

10. Kinetic Modeling of the Post-consumer Poly(Ethylene Terephthalate) Hydrolysis Catalyzed by Cutinase from Humicola insolens

Author:
Erika de Queiros Eugenio; Ivone Sampaio Pereira Campisano; Aline Machado de Castro; Maria Alice Zarur Coelho; Marta Antunes Pereira Langone
Source:
Journal of polymers and the environment 2022 v.30 no.4 pp. 1627-1637
ISSN:
1566-2543
Subject:
Humicola insolens; activation energy; biocatalysts; circular economy; cutinase; employment; enthalpy; entropy; environment; enzymatic hydrolysis; hydrolysis; kinetics; temperature
Abstract:
... The search for a straightforward technology for post-consumer poly(ethylene terephthalate) (PC-PET) degradation is essential to develop a circular economy. In this context, PET hydrolases such as cutinases can be used as bioplatforms for this purpose. Humicola insolens cutinase (HiC) is a promising biocatalyst for PC-PET hydrolysis. Therefore, this work evaluated a kinetic model, and it was observ ...
DOI:
10.1007/s10924-021-02301-4

11. Survival of thermophilic fungi in various preservation methods: A comparative study

Author:
Derya Berikten
Source:
Cryobiology 2021 v.101 pp. 38-43
ISSN:
0011-2240
Subject:
Humicola insolens; Malbranchea; Myceliophthora thermophila; Rhizomucor pusillus; Thermomyces; agar; ambient temperature; biodegradation; comparative study; cryobiology; freeze drying; glucose; liquids; microstructure; starch; thermostable enzymes; tobacco; viability; yeast extract
Abstract:
... Thermophilic fungi have several biotechnological and industrial applications such as thermostable enzyme production, biodegradation, and tobacco processing, etc. Thermophilic fungi cannot survive at temperatures below 20 °C. Owing to their inability to grow at low temperatures, they are not stable, so stocking is very difficult. Although a large number of different storage methods are available an ...
DOI:
10.1016/j.cryobiol.2021.06.005

12. Cutinases as stereoselective catalysts: Specific activity and enantioselectivity of cutinases and lipases for menthol and its analogs

Author:
An Su; Serpil Kiokekli; Mariam Naviwala; Abhijit N. Shirke; Ioannis V. Pavlidis; Richard A. Gross
Source:
Enzyme and microbial technology 2020 v.133 pp. 109467
ISSN:
0141-0229
Subject:
Aspergillus oryzae; Humicola insolens; Pseudozyma antarctica; Rhizomucor miehei; Thermomyces lanuginosus; alcohols; amino acids; aromatic compounds; catalysts; catalytic activity; cutinase; enantioselectivity; hydrophobicity; menthol; moieties
Abstract:
... The specific activity and enantioselectivity of immobilized cutinases from Aspergillus oryzae (AoC) and Humicola insolens (HiC) were compared with those of lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML) and Lipase B from Candida antarctica (CALB) for menthol and its analogs that include isopulegol, trans-2-tert-butylcyclohexanol (2TBC), and dihydrocarveol (DHC). Common feature ...
DOI:
10.1016/j.enzmictec.2019.109467

13. Effects of Methyl Branching on the Properties and Performance of Furandioate-Adipate Copolyesters of Bio-Based Secondary Diols

Author:
Alastair Little; Alessandro Pellis; James W. Comerford; Edwin Naranjo-Valles; Nema Hafezi; Mark Mascal; Thomas J. Farmer
Source:
ACS sustainable chemistry & engineering 2020 v.8 no.38 pp. 14471-14483
ISSN:
2168-0485
Subject:
Humicola insolens; biodegradability; biodegradation; cutinase; enzymatic hydrolysis; esters; glycols; green chemistry; hydrophobicity; polyesters; transesterification
Abstract:
... Furandioate-adipate copolyesters are an emerging class of bio-based biodegradable polymers with great potential to replace fossil-derived terephthalic acid-based copolyesters such as poly(butylene adipate-co-terephthalate) (PBAT). Furandioate-adipate polyesters have almost exclusively been prepared with conventional primary (1°) alcohol diols, while secondary (2°) alcohol diol monomers have largel ...
DOI:
10.1021/acssuschemeng.0c04513

14. Optimizing Immobilization and Stabilization of Feruloyl Esterase from Humicola Insolens and its Application for the Feruloylation of Oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2020 v.98 pp. 11-20
ISSN:
1359-5113
Subject:
Humicola insolens; biocatalysts; biosynthesis; enzyme activity; epoxides; esterification; feruloyl esterase; pH; porosity; response surface methodology; thermal stability; xylooligosaccharides
Abstract:
... Feruloyl esterase (FAE)-catalyzed esterification reaction is as a potential route for the biosynthesis of feruloylated oligosaccharides as functional ingredients. Immobilization of FAE from Humicola insolens on metal chelate-epoxy supports was investigated. The study of effects of immobilization parameters using response surface methodology revealed the significance of enzyme/support ratio (3.25-2 ...
DOI:
10.1016/j.procbio.2020.07.009

15. Process strategies to improve biocatalytic depolymerization of post-consumer PET packages in bioreactors, and investigation on consumables cost reduction

Author:
Adriano Carniel; Absai da Conceição Gomes; Maria Alice Zarur Coelho; Aline Machado de Castro
Source:
Bioprocess and biosystems engineering 2021 v.44 no.3 pp. 507-516
ISSN:
1615-7591
Subject:
Humicola insolens; bioprocessing; bioreactors; cost analysis; cost effectiveness; cutinase; depolymerization; ethylene glycol; fractionation; hydrolysis; manufacturing; pH
Abstract:
... Massive plastics production has raised concerns about low recycling rates and disposal of these materials in nature, causing environmental and economic impacts. Poly(ethylene terephthalate) (PET) is one of main polymers used for manufacture of plastic packaging (e.g. bottles, trays). Enzymatic recycling of PET has been a route of increasing study aiming at to recover its monomers (terephthalic aci ...
DOI:
10.1007/s00449-020-02461-y

16. Production pectin oligosaccharides using Humicola insolens Y1-derived unusual pectate lyase

Author:
Zhiyun Wang; Bo Xu; Huiying Luo; Kun Meng; Yuan Wang; Mengting Liu; Yingguo Bai; Bin Yao; Tao Tu
Source:
Journal of bioscience and bioengineering 2020 v.129 no.1 pp. 16-22
ISSN:
1389-1723
Subject:
Humicola insolens; Komagataella pastoris; anion exchange; apple peels; calcium; electrospray ionization mass spectrometry; enzyme activity; esterification; food industry; fungi; genes; hydrolysates; oligosaccharides; pH stability; pectate lyase; pectins; polymerization; ultra-performance liquid chromatography; wastes
Abstract:
... The economical production of pectin oligosaccharides with a specific degree of polymerization and structure from agro-food waste is an industrially important process. This study identified a novel pectate lyase gene (plhy1) from the thermophilic cellulolytic fungus H. insolens Y1 and tested its ability to produce pectin oligosaccharides. The recombinant PLHY1 produced in Pichia pastoris was superi ...
DOI:
10.1016/j.jbiosc.2019.07.005

17. Enhanced activity towards polyacrylates and poly(vinyl acetate) by site-directed mutagenesis of Humicola insolens cutinase

Author:
Lingqia Su; Ruoyu Hong; Demin Kong; Jing Wu
Source:
International journal of biological macromolecules 2020 v.162 pp. 1752-1759
ISSN:
0141-8130
Subject:
Humicola insolens; cutinase; hydrolysis; mutants; papermaking; polyacrylic acid; porous media; site-directed mutagenesis; thermal stability; turbidity
Abstract:
... Previous studies on the hydrolysis of polyacrylates by cutinase have found that cutinase from Humicola insolens can fulfill the requirement for a thermostable cutinase in the treatment of stickies from papermaking, but it has poor hydrolysis ability. To further improve its ability to hydrolyze the polymers in papermaking, we analyzed the structure of cutinase from H. insolens, and constructed thre ...
DOI:
10.1016/j.ijbiomac.2020.07.261

18. Enzymatic hydrolysis of poly(1,4-butylene 2,5-thiophenedicarboxylate) (PBTF) and poly(1,4-butylene 2,5-furandicarboxylate) (PBF) films: A comparison of mechanisms

Author:
Matteo Gigli; Felice Quartinello; Michelina Soccio; Alessandro Pellis; Nadia Lotti; Georg M. Guebitz; Silvia Licoccia; Andrea Munari
Source:
Environment international 2019 v.130 pp. 104852
ISSN:
0160-4120
Subject:
Humicola insolens; Thermobifida; chemical structure; cleavage (chemistry); cutinase; depolymerization; enzymatic hydrolysis; polyesters
Abstract:
... Enzymatic hydrolysis of poly(1,4-butylene 2,5-thiophenedicarboxylate) (PBTF) and poly(1,4-butylene 2,5-furandicarboxylate) (PBF) by Humicola insolens (HiC) and Thermobifida cellulosilytica (Cut) cutinases is investigated. For the first time, the different depolymerization mechanisms of PBTF (endo-wise scission) and PBF (exo-wise cleavage) has been unveiled and correlated to the chemical structure ...
DOI:
10.1016/j.envint.2019.05.046

19. Immobilized feruloyl esterase from Humicola insolens catalyzes the synthesis of feruloylated oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2019 v.79 pp. 81-90
ISSN:
1359-5113
Subject:
Humicola insolens; acylation; catalytic activity; emulsions; epoxides; ferulic acid; feruloyl esterase; magnetism; microparticles; nanoparticles; raffinose; substrate specificity
Abstract:
... Ferulic acid acylation of oligosaccharides catalyzed by feruloyl esterases (FAE) is a promising route to produce feruloylated oligosaccharides. However, modulation of FAE synthetic properties is a key step to improve the acylation. The efficiency of H. insolens FAE to catalyze the feruloylation in six different surfactantless microemulsions reaction systems was evaluated. The highest yield (57%) w ...
DOI:
10.1016/j.procbio.2018.12.013

20. Unfolding and partial refolding of a cellulase from the SDS-denatured state: From β-sheet to α-helix and back

Author:
Helena Ø. Rasmussen; Jan J. Enghild; Daniel E. Otzen; Jan Skov Pedersen
Source:
Biochimica et biophysica acta 2020 v.1864 no.1 pp. 129434
ISSN:
0304-4165
Subject:
Humicola insolens; beta-lactoglobulin; calorimetry; circular dichroism spectroscopy; detergents; endo-1,4-beta-glucanase; enzyme activity; gel chromatography; industry; micelles; models; neutralization; nonionic surfactants; pH; small-angle X-ray scattering; titration; ultraviolet radiation
Abstract:
... Globular proteins are typically unfolded by SDS to form protein-decorated micelle-like structures. Several proteins have been shown subsequently to refold by addition of the nonionic surfactant octaethylene glycol monododecyl ether (C₁₂E₈). Thus SDS converts β-lactoglobulin, which has mainly β-sheet secondary structure, into a state rich in α-helicality, while addition of C₁₂E₈ leads to refolding ...
DOI:
10.1016/j.bbagen.2019.129434