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1. Enhanced biodegradation activity towards poly(ethyl acrylate) and poly(vinyl acetate) by anchor peptide assistant targeting

Author:
Zhanzhi Liu; Guangyao Li; Fengshan Zhang; Jing Wu
Source:
Journal of biotechnology 2022 v.349 pp. 47-52
ISSN:
0168-1656
Subject:
Escherichia coli; Humicola insolens; biodegradation; biotechnology; cutinase; enzymatic treatment; flocculation; forests; peptides; polyesters; product quality; pulp; pulp and paper industry; raw materials; sediments; waste paper; wood
Abstract:
... The paper industry is one of the most important basic raw material pillar industries. With the decrease of forest wood resources, the recycling of wastepaper has drawn increasingly attention. However, the stickies generated in the process of wastepaper recycling will flocculate and deposite in the pulp, resulting in production accidents and inferior product quality. The biological enzymatic method ...
DOI:
10.1016/j.jbiotec.2022.03.003

2. Enzymatic Epoxidation of Long-Chain Terminal Alkenes by Fungal Peroxygenases

Author:
Esteban D. Babot; Carmen Aranda; Jan Kiebist; Katrin Scheibner; René Ullrich; Martin Hofrichter; Angel T. Martínez; Ana Gutiérrez
Source:
Antioxidants 2022 v.11 no.3 pp. -
ISSN:
2076-3921
Subject:
Agrocybe; Coprinopsis cinerea; Daldinia; Humicola insolens; Marasmius; alkenes; cytochrome P-450; enols; epoxidation reactions; epoxides; fungi; hydroxylation; pH; polymers
Abstract:
... Terminal alkenes are among the most attractive starting materials for the synthesis of epoxides, which are essential and versatile intermediate building blocks for the pharmaceutical, flavoring, and polymer industries. Previous research on alkene epoxidation has focused on the use of several oxidizing agents and/or different enzymes, including cytochrome P450 monooxygenases, as well as microbial w ...
DOI:
10.3390/antiox11030522

3. Novel efficient enzymatic synthesis of the key-reaction intermediate of PET depolymerization, mono(2-hydroxyethyl terephthalate) – MHET

Author:
Erika de Queiros Eugenio; Ivone Sampaio Pereira Campisano; Ayres Guimarães Dias; Aline Machado de Castro; Maria Alice Zarur Coelho; Marta Antunes Pereira Langone
Source:
Journal of biotechnology 2022 v.358 pp. 102-110
ISSN:
0168-1656
Subject:
Humicola insolens; biotechnology; calorimetry; cutinase; depolymerization; enzymatic hydrolysis; enzyme kinetics; ethylene glycol; hydrolysis; mathematical models; nuclear magnetic resonance spectroscopy; polymers; thermogravimetry
Abstract:
... Poly(ethylene terephthalate) (PET) is one of the main synthetic plastics produced worldwide. The extensive use of this polymer causes several problems due to its low degradability. In this scenario, biocatalysts dawn as an alternative to enhance PET recycling. The enzymatic hydrolysis of PET results in a mixture of terephthalic acid (TPA), ethylene glycol (EG), mono-(2-hydroxyethyl) terephthalate ...
DOI:
10.1016/j.jbiotec.2022.08.019

4. Biocatalytic depolymerization of waste polyester mooring lines from oil and gas offshore platforms made of poly(ethylene terephthalate) (PET)

Author:
Aline M de Castro; Adriano Carniel; Sonia M Menezes; Luiz S Chinelatto Júnior; Hercílio de Honorato; Aline P Lima; Erica G Chaves
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.3 pp. 709-718
ISSN:
0268-2575
Subject:
Fourier transform infrared spectroscopy; Humicola insolens; X-radiation; biotechnology; catalytic activity; circular economy; crystal structure; cutinase; depolymerization; molecular weight; nuclear magnetic resonance spectroscopy; oils; pH; pollution; temperature; thermogravimetry
Abstract:
... BACKGROUND: The global concern of plastic pollution in the environment has consistently been emerging in the past years. As one of the main polymers constituting single‐use products, poly(ethylene terephthalate) (PET) is found in a plethora of packages for diverse sectors, and its recycling has been broadly addressed. However, other relevant industrial PET wastes have not been under investigation ...
DOI:
10.1002/jctb.6955

5. CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Author:
Chao Fan; Wei Zhang; Xiaoyun Su; Wangli Ji; Huiying Luo; Yuhong Zhang; Bo Liu; Bin Yao; Huoqing Huang; Xinxin Xu
Source:
Biotechnology for biofuels 2021 v.14 no.1 pp. 206
ISSN:
1754-6834
Subject:
CRISPR-Cas systems; Hepatitis delta virus; Humicola insolens; biofuels; biotechnology; endo-1,4-beta-glucanase; fungi; gene editing; genes; hybrids; promoter regions; transcription factors
Abstract:
... BACKGROUND: Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism. RESULTS: Herein, a CRISPR/Cas9 genome-editing system was established in H. insolens based on a hybri ...
DOI:
10.1186/s13068-021-02057-y

6. Covalent binding and in-situ immobilization of lipases on a flexible nanoporous material

Author:
Saba Ghasemi; Maryam Yousefi; Ahmad Nikseresht; Hoda Omidi
Source:
Process biochemistry 2021 v.102 pp. 92-101
ISSN:
1359-5113
Subject:
Humicola insolens; ambient temperature; carboxylic ester hydrolases; nanopores; pH; thermal stability; ultrasonic treatment
Abstract:
... In this study, the flexible nanoporous MIL-53(Fe) (MIL = Materials of Institute Lavoisier) was used as an efficient support for in-situ and covalent binding immobilization of Humicola insolens lipase (HIL) and Rhizomucour miehei lipase (RML). In the covalent attachment procedure, the support synthesized under ultrasound irradiation was functionalized by N,N-dicyclohexylcarbodiimide and then attach ...
DOI:
10.1016/j.procbio.2020.12.013

7. Experimental and mathematical modeling approaches for biocatalytic post-consumer poly(ethylene terephthalate) hydrolysis

Author:
Erika de Queiros Eugenio; Ivone Sampaio Pereira Campisano; Aline Machado de Castro; Maria Alice Zarur Coelho; Marta Antunes Pereira Langone
Source:
Journal of biotechnology 2021 v.341 pp. 76-85
ISSN:
0168-1656
Subject:
Humicola insolens; biocatalysis; biocatalysts; biotechnology; cutinase; depolymerization; environmental impact; enzymatic hydrolysis; half life; hydrolysis; pH; thermal stability; value-added products; wastes
Abstract:
... The environmental impact arising from poly(ethylene terephthalate) (PET) waste is notable worldwide. Enzymatic PET hydrolysis can provide chemicals that serve as intermediates for value-added product synthesis and savings in the resources. In the present work, some reaction parameters were evaluated on the hydrolysis of post-consumer PET (PC-PET) using a cutinase from Humicola insolens (HiC). The ...
DOI:
10.1016/j.jbiotec.2021.09.007

8. Feruloylation of polysaccharides from cranberry and characterization of their prebiotic properties

Author:
Eugenio Spadoni Andreani; Mingqin Li; Jennifer Ronholm; Salwa Karboune
Source:
Food bioscience 2021 v.42 pp. 101071
ISSN:
2212-4292
Subject:
Bifidobacterium longum; Humicola insolens; Lactobacillus brevis; antioxidants; cranberries; esterification; fatty acid composition; fermentation; feruloyl esterase; inulin; pectins; pomace; prebiotics; propionic acid; raffinose; short chain fatty acids
Abstract:
... Humicola insolens feruloyl esterase was immobilized on chemically modified epoxy-activated supports of different pore sizes. Native and immobilized feruloyl esterases were investigated for their esterification activity on raffinose in surfactantless microemulsions. Pectic polysaccharides, obtained from cranberry pomace using chelating agent (CH) and diluted alkaline (DA) extractions, were then est ...
DOI:
10.1016/j.fbio.2021.101071

9. Glucomannan and beta-glucan degradation by Mytilus edulis Cel45A: Crystal structure and activity comparison with GH45 subfamily A, B and C

Author:
Laura Okmane; Gustav Nestor; Emma Jakobsson; Bingze Xu; Kiyohiko Igarashi; Mats Sandgren; Gerard J. Kleywegt; Jerry Ståhlberg
Source:
Carbohydrate polymers 2022 v.277 pp. 118771
ISSN:
0144-8617
Subject:
Humicola insolens; Mytilus edulis; Phanerochaete chrysosporium; Trichoderma reesei; barley; beta-glucans; beta-mannosidase; carboxymethylcellulose; cellobiose; cold; crystal structure; enzymatic hydrolysis; glucose; glycosides; hydrolysis; konjac mannan; nuclear magnetic resonance spectroscopy
Abstract:
... The enzymatic hydrolysis of barley beta-glucan, konjac glucomannan and carboxymethyl cellulose by a β-1,4-D-endoglucanase MeCel45A from blue mussel, Mytilus edulis, which belongs to subfamily B of glycoside hydrolase family 45 (GH45), was compared with GH45 members of subfamilies A (Humicola insolens HiCel45A), B (Trichoderma reesei TrCel45A) and C (Phanerochaete chrysosporium PcCel45A). Furthermo ...
DOI:
10.1016/j.carbpol.2021.118771

10. Kinetic Modeling of the Post-consumer Poly(Ethylene Terephthalate) Hydrolysis Catalyzed by Cutinase from Humicola insolens

Author:
Erika de Queiros Eugenio; Ivone Sampaio Pereira Campisano; Aline Machado de Castro; Maria Alice Zarur Coelho; Marta Antunes Pereira Langone
Source:
Journal of polymers and the environment 2022 v.30 no.4 pp. 1627-1637
ISSN:
1566-2543
Subject:
Humicola insolens; activation energy; biocatalysts; circular economy; cutinase; employment; enthalpy; entropy; environment; enzymatic hydrolysis; hydrolysis; kinetics; temperature
Abstract:
... The search for a straightforward technology for post-consumer poly(ethylene terephthalate) (PC-PET) degradation is essential to develop a circular economy. In this context, PET hydrolases such as cutinases can be used as bioplatforms for this purpose. Humicola insolens cutinase (HiC) is a promising biocatalyst for PC-PET hydrolysis. Therefore, this work evaluated a kinetic model, and it was observ ...
DOI:
10.1007/s10924-021-02301-4

11. Survival of thermophilic fungi in various preservation methods: A comparative study

Author:
Derya Berikten
Source:
Cryobiology 2021 v.101 pp. 38-43
ISSN:
0011-2240
Subject:
Humicola insolens; Malbranchea; Myceliophthora thermophila; Rhizomucor pusillus; Thermomyces; agar; ambient temperature; biodegradation; comparative study; cryobiology; freeze drying; glucose; liquids; microstructure; starch; thermostable enzymes; tobacco; viability; yeast extract
Abstract:
... Thermophilic fungi have several biotechnological and industrial applications such as thermostable enzyme production, biodegradation, and tobacco processing, etc. Thermophilic fungi cannot survive at temperatures below 20 °C. Owing to their inability to grow at low temperatures, they are not stable, so stocking is very difficult. Although a large number of different storage methods are available an ...
DOI:
10.1016/j.cryobiol.2021.06.005

12. Cutinases as stereoselective catalysts: Specific activity and enantioselectivity of cutinases and lipases for menthol and its analogs

Author:
An Su; Serpil Kiokekli; Mariam Naviwala; Abhijit N. Shirke; Ioannis V. Pavlidis; Richard A. Gross
Source:
Enzyme and microbial technology 2020 v.133 pp. 109467
ISSN:
0141-0229
Subject:
Aspergillus oryzae; Humicola insolens; Pseudozyma antarctica; Rhizomucor miehei; Thermomyces lanuginosus; alcohols; amino acids; aromatic compounds; catalysts; catalytic activity; cutinase; enantioselectivity; hydrophobicity; menthol; moieties
Abstract:
... The specific activity and enantioselectivity of immobilized cutinases from Aspergillus oryzae (AoC) and Humicola insolens (HiC) were compared with those of lipases from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML) and Lipase B from Candida antarctica (CALB) for menthol and its analogs that include isopulegol, trans-2-tert-butylcyclohexanol (2TBC), and dihydrocarveol (DHC). Common feature ...
DOI:
10.1016/j.enzmictec.2019.109467

13. Effects of Methyl Branching on the Properties and Performance of Furandioate-Adipate Copolyesters of Bio-Based Secondary Diols

Author:
Alastair Little; Alessandro Pellis; James W. Comerford; Edwin Naranjo-Valles; Nema Hafezi; Mark Mascal; Thomas J. Farmer
Source:
ACS sustainable chemistry & engineering 2020 v.8 no.38 pp. 14471-14483
ISSN:
2168-0485
Subject:
Humicola insolens; biodegradability; biodegradation; cutinase; enzymatic hydrolysis; esters; glycols; green chemistry; hydrophobicity; polyesters; transesterification
Abstract:
... Furandioate-adipate copolyesters are an emerging class of bio-based biodegradable polymers with great potential to replace fossil-derived terephthalic acid-based copolyesters such as poly(butylene adipate-co-terephthalate) (PBAT). Furandioate-adipate polyesters have almost exclusively been prepared with conventional primary (1°) alcohol diols, while secondary (2°) alcohol diol monomers have largel ...
DOI:
10.1021/acssuschemeng.0c04513

14. Optimizing Immobilization and Stabilization of Feruloyl Esterase from Humicola Insolens and its Application for the Feruloylation of Oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2020 v.98 pp. 11-20
ISSN:
1359-5113
Subject:
Humicola insolens; biocatalysts; biosynthesis; enzyme activity; epoxides; esterification; feruloyl esterase; pH; porosity; response surface methodology; thermal stability; xylooligosaccharides
Abstract:
... Feruloyl esterase (FAE)-catalyzed esterification reaction is as a potential route for the biosynthesis of feruloylated oligosaccharides as functional ingredients. Immobilization of FAE from Humicola insolens on metal chelate-epoxy supports was investigated. The study of effects of immobilization parameters using response surface methodology revealed the significance of enzyme/support ratio (3.25-2 ...
DOI:
10.1016/j.procbio.2020.07.009

15. Process strategies to improve biocatalytic depolymerization of post-consumer PET packages in bioreactors, and investigation on consumables cost reduction

Author:
Adriano Carniel; Absai da Conceição Gomes; Maria Alice Zarur Coelho; Aline Machado de Castro
Source:
Bioprocess and biosystems engineering 2021 v.44 no.3 pp. 507-516
ISSN:
1615-7591
Subject:
Humicola insolens; bioprocessing; bioreactors; cost analysis; cost effectiveness; cutinase; depolymerization; ethylene glycol; fractionation; hydrolysis; manufacturing; pH
Abstract:
... Massive plastics production has raised concerns about low recycling rates and disposal of these materials in nature, causing environmental and economic impacts. Poly(ethylene terephthalate) (PET) is one of main polymers used for manufacture of plastic packaging (e.g. bottles, trays). Enzymatic recycling of PET has been a route of increasing study aiming at to recover its monomers (terephthalic aci ...
DOI:
10.1007/s00449-020-02461-y

16. Production pectin oligosaccharides using Humicola insolens Y1-derived unusual pectate lyase

Author:
Zhiyun Wang; Bo Xu; Huiying Luo; Kun Meng; Yuan Wang; Mengting Liu; Yingguo Bai; Bin Yao; Tao Tu
Source:
Journal of bioscience and bioengineering 2020 v.129 no.1 pp. 16-22
ISSN:
1389-1723
Subject:
Humicola insolens; Komagataella pastoris; anion exchange; apple peels; calcium; electrospray ionization mass spectrometry; enzyme activity; esterification; food industry; fungi; genes; hydrolysates; oligosaccharides; pH stability; pectate lyase; pectins; polymerization; ultra-performance liquid chromatography; wastes
Abstract:
... The economical production of pectin oligosaccharides with a specific degree of polymerization and structure from agro-food waste is an industrially important process. This study identified a novel pectate lyase gene (plhy1) from the thermophilic cellulolytic fungus H. insolens Y1 and tested its ability to produce pectin oligosaccharides. The recombinant PLHY1 produced in Pichia pastoris was superi ...
DOI:
10.1016/j.jbiosc.2019.07.005

17. Enhanced activity towards polyacrylates and poly(vinyl acetate) by site-directed mutagenesis of Humicola insolens cutinase

Author:
Lingqia Su; Ruoyu Hong; Demin Kong; Jing Wu
Source:
International journal of biological macromolecules 2020 v.162 pp. 1752-1759
ISSN:
0141-8130
Subject:
Humicola insolens; cutinase; hydrolysis; mutants; papermaking; polyacrylic acid; porous media; site-directed mutagenesis; thermal stability; turbidity
Abstract:
... Previous studies on the hydrolysis of polyacrylates by cutinase have found that cutinase from Humicola insolens can fulfill the requirement for a thermostable cutinase in the treatment of stickies from papermaking, but it has poor hydrolysis ability. To further improve its ability to hydrolyze the polymers in papermaking, we analyzed the structure of cutinase from H. insolens, and constructed thre ...
DOI:
10.1016/j.ijbiomac.2020.07.261

18. Enzymatic hydrolysis of poly(1,4-butylene 2,5-thiophenedicarboxylate) (PBTF) and poly(1,4-butylene 2,5-furandicarboxylate) (PBF) films: A comparison of mechanisms

Author:
Matteo Gigli; Felice Quartinello; Michelina Soccio; Alessandro Pellis; Nadia Lotti; Georg M. Guebitz; Silvia Licoccia; Andrea Munari
Source:
Environment international 2019 v.130 pp. 104852
ISSN:
0160-4120
Subject:
Humicola insolens; Thermobifida; chemical structure; cleavage (chemistry); cutinase; depolymerization; enzymatic hydrolysis; polyesters
Abstract:
... Enzymatic hydrolysis of poly(1,4-butylene 2,5-thiophenedicarboxylate) (PBTF) and poly(1,4-butylene 2,5-furandicarboxylate) (PBF) by Humicola insolens (HiC) and Thermobifida cellulosilytica (Cut) cutinases is investigated. For the first time, the different depolymerization mechanisms of PBTF (endo-wise scission) and PBF (exo-wise cleavage) has been unveiled and correlated to the chemical structure ...
DOI:
10.1016/j.envint.2019.05.046

19. Immobilized feruloyl esterase from Humicola insolens catalyzes the synthesis of feruloylated oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2019 v.79 pp. 81-90
ISSN:
1359-5113
Subject:
Humicola insolens; acylation; catalytic activity; emulsions; epoxides; ferulic acid; feruloyl esterase; magnetism; microparticles; nanoparticles; raffinose; substrate specificity
Abstract:
... Ferulic acid acylation of oligosaccharides catalyzed by feruloyl esterases (FAE) is a promising route to produce feruloylated oligosaccharides. However, modulation of FAE synthetic properties is a key step to improve the acylation. The efficiency of H. insolens FAE to catalyze the feruloylation in six different surfactantless microemulsions reaction systems was evaluated. The highest yield (57%) w ...
DOI:
10.1016/j.procbio.2018.12.013

20. Unfolding and partial refolding of a cellulase from the SDS-denatured state: From β-sheet to α-helix and back

Author:
Helena Ø. Rasmussen; Jan J. Enghild; Daniel E. Otzen; Jan Skov Pedersen
Source:
Biochimica et biophysica acta 2020 v.1864 no.1 pp. 129434
ISSN:
0304-4165
Subject:
Humicola insolens; beta-lactoglobulin; calorimetry; circular dichroism spectroscopy; detergents; endo-1,4-beta-glucanase; enzyme activity; gel chromatography; industry; micelles; models; neutralization; nonionic surfactants; pH; small-angle X-ray scattering; titration; ultraviolet radiation
Abstract:
... Globular proteins are typically unfolded by SDS to form protein-decorated micelle-like structures. Several proteins have been shown subsequently to refold by addition of the nonionic surfactant octaethylene glycol monododecyl ether (C₁₂E₈). Thus SDS converts β-lactoglobulin, which has mainly β-sheet secondary structure, into a state rich in α-helicality, while addition of C₁₂E₈ leads to refolding ...
DOI:
10.1016/j.bbagen.2019.129434

21. Cutinases catalyze polyacrylate hydrolysis and prevent their aggregation

Author:
Ruoyu Hong; Lingqia Su; Jing Wu
Source:
Polymer degradation and stability 2019 v.159 pp. 23-30
ISSN:
0141-3910
Subject:
Fusarium solani; Humicola insolens; Thermobifida fusca; cutinase; dispersions; hydrolysis; pH; paper recycling; polyacrylic acid; porous media; recycled paper; temperature; turbidity; waste paper
Abstract:
... During the recycling of waste paper, the accumulation of polyacrylates present in the waste paper causes the formation of tacky substances known as stickies. Deposition of these stickies on the machinery decreases the quality of the recycled paper and increases the usage of circulating water. Enzymes that hydrolyze these polyacrylates can minimize or eliminate the deposition of stickies. In initia ...
DOI:
10.1016/j.polymdegradstab.2018.10.028

22. Enzyme-catalyzed simultaneous hydrolysis-glycolysis reactions reveals tunability on PET depolymerization products

Author:
Aline Machado de Castro; Adriano Carniel; Lys Sirelli; Marcos Lopes Dias; Sonia Maria Cabral de Menezes; Luiz Silvino Chinelatto Junior; Hercilio de Angeli Honorato
Source:
Biochemical engineering journal 2018 v.137 pp. 239-246
ISSN:
1369-703X
Subject:
Humicola insolens; Pseudozyma antarctica; catalytic activity; circular economy; cutinase; depolymerization; esterification; ethylene glycol; glycolysis; hydrolysis; plastics; recycling; solvents
Abstract:
... PET depolymerization via enzymatic catalysis is one of the most promising technologies in the context of plastics circular economy. So far, hydrolysis reactions accounted for almost the totality of studies, since the glycolysis route was only recently reported. In the present work, a comparative analysis of hydrolysis and glycolysis of PET oligomers catalyzed by Humicola insolens cutinase (HiC) an ...
DOI:
10.1016/j.bej.2018.06.007

23. High-fold improvement of assorted post-consumer poly(ethylene terephthalate) (PET) packages hydrolysis using Humicola insolens cutinase as a single biocatalyst

Author:
Aline Machado de Castro; Adriano Carniel; Diego Stahelin; Luiz Silvino Chinelatto Junior; Hercilio de Angeli Honorato; Sonia Maria Cabral de Menezes
Source:
Process biochemistry 2019 v.81 pp. 85-91
ISSN:
1359-5113
Subject:
Humicola insolens; biocatalysts; circular economy; cutinase; depolymerization; hydrolysis; plastics; polyethylene terephthalates; recycling
Abstract:
... The dissemination of technologies for poly(ethylene terephthalate) (PET) recycling is of paramount importance in the context of the plastics circular economy. One of the most promising alternatives is to use enzymes as catalysts for PET depolymerization to its monomers, but this route still needs improvement, especially regarding titer and productivity. In the present work, a sequential approach c ...
DOI:
10.1016/j.procbio.2019.03.006

24. High-level expression of Humicola insolens cutinase in Pichia pastoris without carbon starvation and its use in cotton fabric bioscouring

Author:
Ruoyu Hong; Yirong Sun; Lingqia Su; Lengtao Gu; Fuqing Wang; Jing Wu
Source:
Journal of biotechnology 2019 v.304 pp. 10-15
ISSN:
0168-1656
Subject:
Humicola insolens; Komagataella pastoris; Thermobifida fusca; bioreactors; bioscouring; butyrates; carbon; cell growth; cotton fabric; cutinase; enzyme activity; fermentation; glycerol; methanol; monitoring; sorbitol; starvation; wettability
Abstract:
... Huimcola insolens cutinase (HiC) was heterologously expressed in Pichia pastoris. To avoid a carbon starvation step, fermentation was conducted using combinations of sorbitol with glycerol and methanol in the cell growth and induction phases, respectively. The cutinase productivity (27.71 U mL−1 h−1) was 9.93 U mL−1 h−1 greater than that achieved using traditional two-phase methods, and a cutinase ...
DOI:
10.1016/j.jbiotec.2019.07.011

25. Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides

Author:
Jinyin Chen; Xiuna Guo; Min Zhu; Chen Chen; Duochuan Li
Source:
Biotechnology for biofuels 2019 v.12 no.1 pp. 42
ISSN:
1754-6834
Subject:
Humicola insolens; Komagataella pastoris; beta-glucosidase; beta-glucuronidase; catalytic activity; cellulose; genes; glucose; glucuronic acid; hydrolysis; moieties; oligosaccharides; oxidation
Abstract:
... BACKGROUND: Polysaccharide monooxygenases (PMOs) play an important role in the enzymatic degradation of cellulose. They have been demonstrated to able to C6-oxidize cellulose to produce C6-hexodialdoses. However, the biological function of C6 oxidation of PMOs remains unknown. In particular, it is unclear whether C6-hexodialdoses can be further oxidized to uronic acid (glucuronic acid-containing o ...
DOI:
10.1186/s13068-019-1384-0
PubMed:
30858879
PubMed Central:
PMC6391835

26. Switched reaction specificity in polyesterases towards amide bond hydrolysis by enzyme engineering

Author:
Antonino Biundo; Raditya Subagia; Michael Maurer; Doris Ribitsch; Per-Olof Syrén; Georg M. Guebitz
Source:
RSC advances 2019 v.9 no.62 pp. 36217-36226
ISSN:
2046-2069
Subject:
Humicola insolens; Thermobifida; amidase; amides; biocatalysts; catalytic activity; cutinase; depolymerization; engineering; enzyme activity; hydrogen bonding; hydrolysis; microplastics; molecular dynamics; nylon; polyamides; polyethylene terephthalates; recycling
Abstract:
... The recalcitrance of plastics like nylon and other polyamides contributes to environmental problems (e.g. microplastics in oceans) and restricts possibilities for recycling. The fact that hitherto discovered amidases (EC 3.5.1. and 3.5.2.) only show no, or low, activity on polyamides currently obstructs biotechnological-assisted depolymerization of man-made materials. In this work, we capitalized ...
DOI:
10.1039/c9ra07519d

27. The use of Agrobacterium-mediated insertional mutagenesis sequencing to identify novel genes of Humicola insolens involved in cellulase production

Author:
Chao Fan; Xinxin Xu; Liya Song; Weishi Guan; Jinyang Li; Bo Liu; Pengjun Shi; Wei Zhang
Source:
3 Biotech 2018 v.8 no.3 pp. 153
ISSN:
2190-572X
Subject:
Humicola insolens; bioinformatics; endo-1,4-beta-glucanase; enzyme activity; fungi; genes; insertional mutagenesis; liquid state fermentation; mass spectrometry; mutants; polyacrylamide gel electrophoresis; proteins; transfer DNA; xylanases
Abstract:
... A transfer DNA (T-DNA)-tagged mutant library of Humicola insolens was screened for mutants with altered cellulase production using the plate-clearing zone assay. Three selected mutants (5-A7, 5-C6, and 13-B7) exhibited significantly depressed FPase, CMCase and xylanase activities compared with the wild-type strain upon shake-flask fermentation, while the pNPCase and pNPGase activities of the three ...
DOI:
10.1007/s13205-018-1166-6
PubMed:
29492372
PubMed Central:
PMC5829266

28. A versatile Trichoderma reesei expression system for the production of heterologous proteins

Author:
Jiajia Zhang; Chuan Wu; Wei Wang; Dongzhi Wei
Source:
Biotechnology letters 2018 v.40 no.6 pp. 965-972
ISSN:
0141-5492
Subject:
Humicola insolens; Trichoderma reesei; carbon; endo-1,4-beta-glucanase; red fluorescent protein; reporter genes; teleomorphs
Abstract:
... OBJECTIVES: To develop a versatile Trichoderma reesei (teleomorph Hypocrea jecorina) expression system for the high-purity production of heterologous proteins. RESULTS: The versatile T. reesei expression system is based on xyn1 and xyn2 promoters, A824V transition in XYRI, and a bicomponent carbon source strategy. Red fluorescent protein gene rfp and alkaline endoglucanase EGV gene egv3 from Humic ...
DOI:
10.1007/s10529-018-2548-x

29. Heterologous expression and biochemical studies of a thermostable glucose tolerant β-glucosidase from Methylococcus capsulatus (bath strain)

Author:
Sneha S. Sathe; Surabhi Soni; Vikas P. Ranvir; Vikram G. Choudhari; Annamma A. Odaneth; Arvind M. Lali; Sanjeev K. Chandrayan
Source:
International journal of biological macromolecules 2017 v.102 pp. 805-812
ISSN:
0141-8130
Subject:
Humicola insolens; Methylococcus capsulatus; active sites; beta-glucosidase; biomass; cellobiose; detergents; glucose; glucose tolerance; heterologous gene expression; hydrolysis; models; pH; rice straw; solvents; temperature; thermal stability
Abstract:
... Glucose inhibition of β-glucosidase (BG) is a bottleneck in biomass hydrolysis. In this study, a glucose resistant GH1 β-glucosidase gene- Mbgl from Methylococcus capsulatus (bath strain) was cloned and overexpressed in E.coli. The Ni-NTA affinity purified Mbgl displayed an optimum temperature of 70°C and optimum pH was 6.0. The calculated KM of the enzyme was 48.6mM and 0.12mM for cellobiose and ...
DOI:
10.1016/j.ijbiomac.2017.04.078

30. Immobilized cutinases: Preparation, solvent tolerance and thermal stability

Author:
An Su; Abhijit Shirke; Joshua Baik; Yi Zou; Richard Gross
Source:
Enzyme and microbial technology 2018 v.116 pp. 33-40
ISSN:
0141-0229
Subject:
Aspergillus oryzae; Humicola insolens; Thielavia; acetates; butanol; catalysts; cutinase; dodecanoic acid; hydrophobic bonding; immobilized enzymes; mixing; phosphates; porous media; solvents; substrate specificity; thermal stability
Abstract:
... Developing active immobilized enzymes and characterization of their use conditions is critically important prior to initiating studies of enzyme selectivity and substrate specificity in organic media. To this end, physical immobilization by hydrophobic interactions was performed with three well-characterized cutinases (Aspergillus oryzae Cutinase (AoC), Humicola insolens Cutinase (HiC), and Thiela ...
DOI:
10.1016/j.enzmictec.2018.05.010

31. Lipase from Candida antarctica (CALB) and cutinase from Humicola insolens act synergistically for PET hydrolysis to terephthalic acid

Author:
Adriano Carniel; Érika Valoni; José Nicomedes; Absai da Conceição Gomes; Aline Machado de Castro
Source:
Process biochemistry 2017 v.59 pp. 84-90
ISSN:
1359-5113
Subject:
Humicola insolens; Pseudozyma antarctica; biocatalysts; cutinase; depolymerization; ethylene; hydrolysis; plastics; polyethylene terephthalates; recycling; screening; temperature
Abstract:
... Poly(ethylene terephthalate) (PET) is one of the most important industrial plastics in the world. The development of technologies for its depolymerization aiming at monomer recycling is of paramount interest and in this context enzyme-catalyzed hydrolysis has been recognized as a promising alternative. In the present study, a screening of 10 commercial enzymes using bis-(hydroxyethyl) terephthalat ...
DOI:
10.1016/j.procbio.2016.07.023

32. Neutral and alkaline cellulases: Production, engineering, and applications

Author:
Ines Ben Hmad; Ali Gargouri
Source:
Journal of basic microbiology 2017 v.57 no.8 pp. 653-658
ISSN:
0233-111X
Subject:
Humicola insolens; cellulases; engineering; fabrics; feeds; fungi; laundry; pH; pulp and paper industry; screening; wines
Abstract:
... Neutral and alkaline cellulases from microorganisms constitute a major group of the industrial enzymes and find applications in various industries. Screening is the important ways to get novel cellulases. Most fungal cellulases have acidic pH optima, except some fungi like Humicola insolens species. However, new applications require the use of neutral and alkaline cellulases in food, brewery and w ...
DOI:
10.1002/jobm.201700111

33. Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources

Author:
Aline Machado de Castro; Adriano Carniel; José Nicomedes Junior; Absai da Conceição Gomes; Érika Valoni
Source:
Journal of industrial microbiology & biotechnology 2017 v.44 no.6 pp. 835-844
ISSN:
1367-5435
Subject:
Humicola insolens; Pseudozyma antarctica; cutinase; depolymerization; ethylene; hydrolysis; plastics; polyethylene terephthalates; recycling; screening
Abstract:
... Poly(ethylene terephthalate) (PET) is one of the most consumed plastics in the world. The development of efficient technologies for its depolymerization for monomers reuse is highly encouraged, since current recycling rates are still very low. In this study, 16 commercial lipases and cutinases were evaluated for their abilities to catalyze the hydrolysis of two PET samples. Humicola insolens cutin ...
DOI:
10.1007/s10295-017-1942-z

34. A novel process for poly(ethylene terephthalate) depolymerization via enzyme-catalyzed glycolysis

Author:
Aline Machado de Castro; Adriano Carniel
Source:
Biochemical engineering journal 2017 v.124 pp. 64-68
ISSN:
1369-703X
Subject:
Humicola insolens; Pseudozyma antarctica; cutinase; depolymerization; esterification; glycolysis; hydrolysis; polyethylene terephthalates; recycling; temperature
Abstract:
... The discovery and improvement of processes for effective recycling of poly(ethylene terephthalate) (PET) packages meet high-priority and short-term demands for the implementation of a circular economy.One of the most interesting approaches is enzyme-catalyzed depolymerization, which to date has been exclusively reported as hydrolysis reactions. In this paper, we describe a novel route for PET depo ...
DOI:
10.1016/j.bej.2017.04.011

35. Functionalized Celluloses with Regular Substitution Pattern by Glycosynthase-Catalyzed Polymerization

Author:
Codera Victoria; Edgar Kevin J.; Faijes Magda; Planas Antoni
Source:
Biomacromolecules 2016 v.17 no.4 pp. 1272-1279
ISSN:
1526-4602
Subject:
Humicola insolens; biocompatible materials; biosynthesis; cellulose; endo-1,4-beta-glucanase; mutants; polymerization; polymers; synthesis
Abstract:
... Control of the monomer sequence in polymers is extraordinarily difficult by chemical synthesis, though Nature routinely exerts such control, including in the biosynthesis of polysaccharides. This inability has prevented us from being able to match the exquisite structure–activity control exhibited in biosynthesis of bioactive natural polysaccharides. We here address a powerful approach, whereby en ...
DOI:
10.1021/acs.biomac.5b01453
CHORUS:
10.1021/acs.biomac.5b01453

36. Mycothermus thermophilus (Syn. Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed

Author:
Neha Basotra; Baljit Kaur; Marcos Di Falco; Adrian Tsang; Bhupinder Singh Chadha
Source:
Bioresource technology 2016 v.222 pp. 413-421
ISSN:
0960-8524
Subject:
Humicola insolens; Scytalidium; bagasse; beta-glucosidase; carbohydrate binding; cellulose 1,4-beta-cellobiosidase; databases; endo-1,4-beta-glucanase; esterases; gene expression regulation; hydrolysis; lignocellulose; lyases; mass spectrometry; proteins; rice straw; sugars; thermophilic fungi; xylanases
Abstract:
... Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium. The sequential and differential expression profile of endoglucanases, β-glucosidases, cellobiohydrolases and xylanases using zymogr ...
DOI:
10.1016/j.biortech.2016.10.018

37. Production of rice straw hydrolysis enzymes by the fungi Trichoderma reesei and Humicola insolens using rice straw as a carbon source

Author:
Takashi Kogo; Yuki Yoshida; Keisuke Koganei; Hitoshi Matsumoto; Taisuke Watanabe; Jun Ogihara; Takafumi Kasumi
Source:
Bioresource technology 2017 v.233 pp. 67-73
ISSN:
0960-8524
Subject:
Humicola insolens; Trichoderma reesei; carbon; cellulose; crop production; enzyme activity; enzymes; fungi; hydrolysis; rice straw
Abstract:
... Rice straw was evaluated as a carbon source for the fungi, Trichoderma reesei and Humicola insolens, to produce enzymes for rice straw hydrolysis. The enzyme activity of T. reesei and H. insolens cultivated in medium containing non-treated rice straw were almost equivalent to the enzyme of T. reesei cultivated in Avicel medium, a form of refined cellulose. The enzyme activity of T. reesei cultivat ...
DOI:
10.1016/j.biortech.2017.01.075

38. Substrate specificities of cutinases on aliphatic–aromatic polyesters and on their model substrates

Author:
Veronika Perz; Klaus Bleymaier; Carsten Sinkel; Ulf Kueper; Melanie Bonnekessel; Doris Ribitsch; Georg M. Guebitz
Source:
New biotechnology 2016 v.33 no.2 pp. 295-304
ISSN:
1871-6784
Subject:
Humicola insolens; Thermobifida; adipic acid; alcohols; benzoic acid; biodegradability; composts; cutinase; differential scanning calorimetry; enzymatic hydrolysis; esters; fungi; hydrolysis; melting point; polyesters; temperature; thermogravimetry
Abstract:
... The enzymatic hydrolysis of the biodegradable polyester ecoflex and of a variety of oligomeric and polymeric ecoflex model substrates was investigated. For this purpose, substrate specificities of two enzymes of typical compost inhabitants, namely a fungal cutinase from Humicola insolens (HiC) and a bacterial cutinase from Thermobifida cellulosilytica (Thc_Cut1) were compared. Model substrates wer ...
DOI:
10.1016/j.nbt.2015.11.004

39. Enhanced production of heterologous proteins by the filamentous fungus Trichoderma reesei via disruption of the alkaline serine protease SPW combined with a pH control strategy

Author:
Guoxiu Zhang; Yao Zhu; Dongzhi Wei; Wei Wang
Source:
Plasmid 2014 v.71 pp. 16-22
ISSN:
0147-619X
Subject:
Humicola insolens; Trichoderma reesei; bovine serum albumin; endo-1,4-beta-glucanase; enzyme activity; fungi; gene expression; genes; heterologous gene expression; nitrogen; pH; plasmids; post-translational modification; quantitative polymerase chain reaction; secretion; serine proteinases; transcription (genetics)
Abstract:
... The filamentous fungus Trichoderma reesei has received attention as a host for heterologous protein production because of its high secretion capacity and eukaryotic post-translational modifications. However, the heterologous production of proteins in T. reesei is limited by its high expression of proteases. The pH control strategies have been proposed for eliminating acidic, but not alkaline, prot ...
DOI:
10.1016/j.plasmid.2014.01.001

40. Enzymatic degradation of Elephant grass (Pennisetum purpureum) stems: Influence of the pith and bark in the total hydrolysis

Author:
Marta Pérez-Boada; Alicia Prieto; Pepijn Prinsen; Marie-Pierre Forquin-Gomez; José Carlos del Río; Ana Gutiérrez; Ángel T. Martínez; Craig B. Faulds
Source:
Bioresource technology 2014 v.167 pp. 469-475
ISSN:
0960-8524
Subject:
cortex; feruloyl esterase; acetic acid; energy; reducing sugars; stems; glucose; xylan; xylanases; dimethyl sulfoxide; xylose; pith; Cenchrus purpureus; solubility; hydrolysis; Humicola insolens; biomass; bark; solubilization; protective effect
Abstract:
... The internal pith of a high energy plant, Elephant grass (EG), was more extensively degraded (>50% dry matter) compared to the outer cortex (31%) or the whole stem (35%) by an enzyme preparation from Humicola insolens, Ultraflo. Reducing sugars and acetic acid release from the pith was also higher compared to the cortex. Supplementation of Ultraflo with a type-C feruloyl esterase increased the lev ...
DOI:
10.1016/j.biortech.2014.06.018

41. Gene cloning, expression and biochemical characterization of a glucose- and xylose-stimulated β-glucosidase from Humicola insolens RP86

Author:
Flavio Henrique Moreira Souza; Luana Parras Meleiro; Carla Botelho Machado; Ana Lucia Ribeiro Latorre Zimbardi; Raquel Fonseca Maldonado; Tatiana Arruda Campos Brasil Souza; Douglas Chodi Masui; Mario Tyago Murakami; João Atilio Jorge; Richard John Ward; Rosa Prazeres Melo Furriel
Source:
Journal of Molecular Catalysis. B, Enzymatic 2014 v.106 pp. 1-10
ISSN:
1381-1177
Subject:
Escherichia coli; Humicola insolens; beta-glucosidase; biomass; catalytic activity; cellobiose; cellulose; cost effectiveness; enzyme activity; ethanol production; genes; glucose; hydrolysis; industrial applications; light scattering; lignocellulose; molecular cloning; pH; saccharification; substrate specificity; temperature; xylose
Abstract:
... Efficient cellulose saccharification is crucial for the cost-effective production of ethanol from lignocellulosic biomass. Most β-glucosidases are inhibited by glucose, and high enzyme loads are needed to attain acceptable hydrolysis yields, increasing the process costs. Therefore, glucose-stimulated β-glucosidases with high catalytic efficiency for cellobiose hydrolysis are highly interesting, pa ...
DOI:
10.1016/j.molcatb.2014.04.007

42. A New GH43 α-Arabinofuranosidase from Humicola insolens Y1: Biochemical Characterization and Synergistic Action with a Xylanase on Xylan Degradation

Author:
Xinzhuo Yang; Pengjun Shi; Rui Ma; Huiying Luo; Huoqing Huang; Peilong Yang; Bin Yao
Source:
Applied biochemistry and biotechnology 2015 v.175 no.4 pp. 1960-1970
ISSN:
0273-2289
Subject:
sugar beet; arabinoxylan; Komagataella pastoris; xylan; xylanases; wheat; biotechnology; genes; active sites; pH; signal peptide; Humicola insolens; glycosides; alpha-N-arabinofuranosidase
Abstract:
... A new α-arabinofuranosidase gene (Hiabf43) was cloned from Humicola insolens Y1 and successfully expressed in Pichia pastoris GS115. Deduced HiAbf43 contained a putative signal peptide and a catalytic domain of glycoside hydrolase (GH) family 43. Purified recombinant HiAbf43 showed optimal activity at pH 5.0 and 50 °C, and was active over a broad pH range. The enzyme was specific for the cleavage ...
DOI:
10.1007/s12010-014-1416-y

43. Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production

Author:
Hitoshi Matsumoto; Keisuke Koganei; Naoko Nishida; Yoshiyuki Koyama; Sanshiro Saito; Hisashi Kataoka; Jun Ogihara; Takafumi Kasumi
Source:
Journal of bioscience and bioengineering 2014 v.117 pp. 257-262
ISSN:
1389-1723
Subject:
Aspergillus; Humicola insolens; Trichoderma; biomass; carbon; cell growth; cellular microenvironment; cohesion; corn stover; culture media; enzymes; extrusion; fungi; glucose; lignocellulose; mycelium; nitrogen; rice straw; soybean meal; wood chips
Abstract:
... We have developed a method for the effective growth of Humicola insolens conducive to efficient enzyme production using a medium containing glucose as a carbon source and extruded soybean meal (ExSBM) as a nitrogen source. Enzymes from Humicola sp. hold promise for biomass degradation, especially of lignocellulosic materials such as rice straw, wood chips, and corn stover. The strain, however, is ...
DOI:
10.1016/j.jbiosc.2013.08.014

44. Glucose and xylose stimulation of a β-glucosidase from the thermophilic fungus Humicola insolens: A kinetic and biophysical study

Author:
Flavio Henrique Moreira Souza; Rodrigo Furriel Inocentes; Richard John Ward; João Atílio Jorge; Rosa Prazeres Melo Furriel
Source:
Journal of Molecular Catalysis. B, Enzymatic 2013 v.94 pp. 119-128
ISSN:
1381-1177
Subject:
Humicola insolens; active sites; beta-glucosidase; binding sites; catalytic activity; cellulose; enzyme activity; ethanol production; fluorescence; glucose; hydrolysis; light scattering; models; saccharification; thermophilic fungi; tryptophan; xylose
Abstract:
... β-Glucosidases activated by glucose and xylose are uncommon yet intriguing enzymes that may enhance cellulose saccharification efficiency, and are of interest for application in bioethanol production processes. The molecular mechanisms of activation are completely unknown, and the aim of this study was the kinetic and biophysical characterization of the stimulation of a β-glucosidase from Humicola ...
DOI:
10.1016/j.molcatb.2013.05.012

45. High expression of a neutral endo-β-glucanase gene from Humicola insolens in Trichoderma reesei

Author:
Bintao Gu; Liming Xia
Source:
Journal of industrial microbiology & biotechnology 2013 v.40 no.7 pp. 773-779
ISSN:
1367-5435
Subject:
screening; hygromycin B; fermentation; conidia; secretion; fermenters; polyacrylamide gel electrophoresis; Agrobacterium radiobacter; cellulose 1,4-beta-cellobiosidase; polymerase chain reaction; plasmids; carbon; textile industry; genes; pH; sodium; Humicola insolens; Trichoderma reesei; carboxymethylcellulose
Abstract:
... The neutral endo-β-glucanase gene cel5A from Humicola insolens was cloned and connected with the cellobiohydrolase 1 promoter from Trichoderma reesei to construct a recombinant plasmid pCB-hEG with the hygromycin B resistance marker. The plasmid was introduced into conidia of T. reesei using the Agrobacterium tumefaciens mediated transformation method. Eight transformants were obtained on screenin ...
DOI:
10.1007/s10295-013-1267-5
PubMed:
23619969

46. A Novel β-Glucosidase from Humicola insolens with High Potential for Untreated Waste Paper Conversion to Sugars

Author:
Luana Parras Meleiro; Ana Lucia Ribeiro Latorre Zimbardi; Flavio Henrique Moreira Souza; Douglas Chodi Masui; Tony Marcio Silva; João Atilio Jorge; Rosa Prazeres Melo Furriel
Source:
Applied biochemistry and biotechnology 2014 v.173 no.2 pp. 391-408
ISSN:
0273-2289
Subject:
manganese; lead; waste paper; paper; feedstocks; mass spectrometry; Chaetomium; Humicola insolens; cardboard; gel electrophoresis; solid state fermentation; glucose; iron; sodium; beta-glucosidase; cobalt; copper; cellobiose; amino acid sequences; ethanol production; hydrolysis; temperature; Trichoderma reesei; mercury; pH; sequence homology
Abstract:
... Humicola insolens produced a new β-glucosidase (BglHi2) under solid-state fermentation. The purified enzyme showed apparent molecular masses of 116 kDa (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) and 404 kDa (gel-filtration), suggesting that it is a homotetramer. Mass spectrometry analysis showed amino acid sequence similarity with a β-glucosidase from Chaetomium thermophilum. Opti ...
DOI:
10.1007/s12010-014-0847-9
PubMed:
24627121

47. Role of cysteine residues in thermal inactivation of fungal Cel6A cellobiohydrolases

Author:
Indira Wu; Thomas Heel; Frances H. Arnold
Source:
BBA - Proteins and Proteomics 2013 v.1834 pp. 1539-1544
ISSN:
1570-9639
Subject:
Humicola insolens; Trichoderma reesei; cellulases; circular dichroism spectroscopy; cysteine; disulfide bonds; feedstocks; fuels; fungi; heat inactivation; heat treatment; lignocellulose; pH; protein engineering; temperature; thermal stability
Abstract:
... Numerous protein engineering studies have focused on increasing the thermostability of fungal cellulases to improve production of fuels and chemicals from lignocellulosic feedstocks. However, the engineered enzymes still undergo thermal inactivation at temperatures well below the inactivation temperatures of hyperthermophilic cellulases. In this report, we investigated the role of free cysteines i ...
DOI:
10.1016/j.bbapap.2013.05.003

48. Two xylose-tolerant GH43 bifunctional β-xylosidase/α-arabinosidases and one GH11 xylanase from Humicola insolens and their synergy in the degradation of xylan

Author:
Xinzhuo Yang; Pengjun Shi; Huoqing Huang; Huiying Luo; Yaru Wang; Wei Zhang; Bin Yao
Source:
Food chemistry 2014 v.148 pp. 381-387
ISSN:
0308-8146
Subject:
Humicola insolens; biomass; genes; hosts; hydrolysis; nucleotide sequences; pH; synergism; temperature; xylan; xylanases; xylose
Abstract:
... Two β-xylosidases of family 43 (Xyl43A and Xyl43B) and one xylanase of family 11 (Xyn11A) were identified from the genome sequence of Humicola insolens Y1, and their gene products were successfully expressed in heterologous hosts. The optimal activities of the purified Xyl43A, Xyl43B, and Xyn11A were found at pH 6.5–7.0 and 50–60°C. They were stable over a pH range of 5.0–10.0 and temperatures of ...
DOI:
10.1016/j.foodchem.2013.10.062
PubMed:
24262572

49. Characterization of three novel thermophilic xylanases from Humicola insolens Y1 with application potentials in the brewing industry

Author:
Yanlong Du; Pengjun Shi; Huoqing Huang; Xiu Zhang; Huiying Luo; Yaru Wang; Bin Yao
Source:
Bioresource technology 2013 v.130 pp. 161-167
ISSN:
0960-8524
Subject:
temperature; thermal stability; mashing; substrate specificity; Komagataella pastoris; xylanases; brewing industry; viscosity; amino acid sequences; filtration; genes; pH; Humicola insolens; fungi
Abstract:
... Three xylanase genes (xynA, xynB, xynC) of glycosyl hydrolase family 10 were identified in Humicola insolens Y1. The deduced protein sequences showed the highest identity of ⩽83% to known fungal xylanases and of ⩽38% with each other. Recombinant XynA–C produced in Pichia pastoris showed optimal activities at pH 6.0–7.0 and at high temperature (70–80°C), and exhibited good stability over a broad pH ...
DOI:
10.1016/j.biortech.2012.12.067
PubMed:
23306124

50. Chemo-enzymatic synthesis of xylogluco-oligosaccharides and their interactions with cellulose

Author:
Marie Lopez; Sébastien Fort; Hervé Bizot; Alain Buléon; Hugues Driguez
Source:
Carbohydrate polymers 2012 v.88 no.1 pp. 185-193
ISSN:
0144-8617
Subject:
Humicola insolens; cellulose; enzymatic hydrolysis; oligosaccharides; xyloglucans
Abstract:
... A novel class of complex oligosaccharides with a backbone made of twelve β-(1→4)-d-glucosyl units that may be regularly substituted on their primary position with α-d-xylosyl or β-d-(1→2)-galactosyl-α-d-xylosyl residues were readily synthesized using enzymatic hydrolysis of the plant polysaccharide xyloglucan (XG), followed by chemical modifications of the well-defined oligosaccharides and their e ...
DOI:
10.1016/j.carbpol.2011.11.085