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1. Antibody profiling using a recombinant protein–based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model

Author:
Tomomasa Matsuyama; Natsumi Sano; Tomokazu Takano; Takamitsu Sakai; Motoshige Yasuike; Atushi Fujiwara; Yasuhiko Kawato; Jun Kurita; Kazunori Yoshida; Yukinori Shimada; Chihaya Nakayasu
Source:
Vaccine 2018 v.36 no.19 pp. 2643-2649
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
Abstract:
... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
DOI:
10.1016/j.vaccine.2018.03.059

2. Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Author:
Xiao-Peng Xiong; Chuan-Fu Dong; Shao-Ping Weng; Jing Zhang; Ye Zhang; Jian-Guo He
Source:
Fish & shellfish immunology 2011 v.31 no.6 pp. 919-924
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Western blotting; antiserum; coat proteins; financial economics; fish culture; freshwater fish; gel electrophoresis; immune response; industry; mortality; neutralization; proteome; proteomics; structural proteins; vaccines; virion; Asia
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), belonging to the genus Megalocytivirus in the family Iridoviridae, is one of the major agents causing mortality and economic losses to the freshwater fish culture industry in Asian countries. Currently, little information regarding the antigenic properties of Megalocytivirus (especially ISKNV) is available. Our previous study using four differen ...
DOI:
10.1016/j.fsi.2011.08.009

3. Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi

Author:
Qiang Lin; Xiaozhe Fu; Lihui Liu; Hongru Liang; Huizhi Guo; Shuwen Yin; Venkatesh Kumaresan; Zhibin Huang; Ningqiu Li
Source:
Microbial pathogenesis 2017 v.107 pp. 98-105
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; financial economics; genes; monitoring; quantitative polymerase chain reaction; spleen; tissues
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is one of the major epidemiological agents that had caused great economic loss in Chinese perch (Siniperca chuatsi). In this study, a specific TaqMan real-time PCR was developed using a pair of primers and a TaqMan probe specific to the ORF007 gene of ISKNV to rapidly detect ISKNV copies in Chinese perch samples. This assay was optimized to produ ...
DOI:
10.1016/j.micpath.2017.02.046

4. Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line

Author:
Chen Li; Xiaozhe Fu; Qiang Lin; Lihui Liu; Hongru Liang; Zhibin Huang; Ningqiu Li
Source:
Fish & shellfish immunology 2017 v.60 pp. 25-32
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; autophagy; chloroquine; cytoplasm; industry; pathogenesis; proteins; virus replication; viruses
Abstract:
... Autophagy plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of infectious kidney and spleen necrosis virus (ISKNV), an agent that has caused devastating losses in Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection triggered the complete autophagic process, as demonstrated by microtubule-as ...
DOI:
10.1016/j.fsi.2016.11.037

5. Black rockfish C-type lectin, SsCTL4: A pattern recognition receptor that promotes bactericidal activity and virus escape from host immune defense

Author:
Du Xue; Wang Guang-hua; Su Yan-li; Zhang Min; Hu Yong-hua
Source:
Fish & shellfish immunology 2018 v.79 pp. 340-350
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Gram-positive bacteria; Infectious spleen and kidney necrosis virus; Sebastes melanops; Sebastes schlegelii; Vibrio anguillarum; antibacterial properties; bacterial infections; calcium; cysteine; fish; humans; immune response; immunologic receptors; lectins; macrophages; mannose; open reading frames; shellfish; tissues; viruses
Abstract:
... C-type lectin (CTL) is an immune receptor and is received extensive attention of its important roles in immune response and immune escape. Some CTL, such as CTL4, has been well characterized in human and several other mammals, but much less documentation exists about the immunological function of CTL4 in lower vertebrates. In the present study, a C-type lectin domain family 4 member, SsCTL4, which ...
DOI:
10.1016/j.fsi.2018.05.033

6. Characterization and function of mandarin fish c-Myc during viral infection process

Author:
Caimei Ye; Ningqiu Li; Yinjie Niu; Qiang Lin; Xia Luo; Hongru Liang; Lihui Liu; Xiaozhe Fu
Source:
Fish & shellfish immunology 2022 v.120 pp. 686-694
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Rhabdoviridae; Siniperca chuatsi; amino acid sequences; brain; fish; glutamine; immunology; kidneys; metabolism; open reading frames; protein content; shellfish; spleen; transcription factors; virus replication
Abstract:
... c-Myc is a transcription factor and master regulator of cellular metabolism, and plays a critical role in virus replication by regulating glutamine metabolism. In this study, the open-reading frame (ORF) of c-Myc, designated as Sc-c-Myc, was cloned and sequenced. Multiple alignment of the amino acid sequence showed that the conserved domain of Sc-c-Myc, including the helix–loop–helix-zipper (bHLHz ...
DOI:
10.1016/j.fsi.2021.12.045

7. Characterization of a membrane protein (VP001L) from infectious spleen and kidney necrosis virus (ISKNV)

Author:
Xiaopeng Xu; Jing Lu; Qingxia Lu; Hui Zhong; Shaoping Weng; Jianguo He
Source:
Virus genes 2008 v.36 no.1 pp. 157-167
ISSN:
0920-8569
Subject:
messenger RNA; open reading frames; models; transfection; membrane proteins; Infectious spleen and kidney necrosis virus; hydrophobicity; mutants; plasma membrane; start codon; topology
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the type species of megalocytivirus, Iridoviridae. A novel membrane protein corresponding to the first open reading frame (ORF001L) of ISKNV genome was identified. This 378-residue protein, termed the VP001L protein, has a high content of hydrophobic sequences and contains 10–11 putative transmembrane domains, indicating it may be a membrane p ...
DOI:
10.1007/s11262-007-0177-x
PubMed:
18046636

8. Characterization of gene expression profiles and functional analysis of peptidoglycan recognition protein 2 from rock bream (Oplegnathus fasciatus)

Author:
Kwang-Min Choi; Min-Soo Joo; Dong Hee Cho; Jin-Sol Bae; Ji-Min Jung; Jee Youn Hwang; Mun-Gyeong Kwon; Jung Soo Seo; Seong Don Hwang; Bo-Yeong Jee; Do-Hyung Kim; Chan-Il Park
Source:
Fish & shellfish immunology 2019 v.84 pp. 1068-1074
ISSN:
1050-4648
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; amino acids; antimicrobial properties; bacteria; bacterial growth; bacterial infections; bream; cell membranes; complementary DNA; enzymes; gene expression; high-throughput nucleotide sequencing; intestines; liver; open reading frames; peptidoglycans; phagocytosis; phylogeny; protein domains; zinc
Abstract:
... Peptidoglycan recognition protein 2 (PGRP2) is a Zn2+-dependent peptidase that plays important roles in binding to microbial components of the cell membrane, inducing phagocytosis and antimicrobial activity. Rock bream (Oplegnathus fasciatus) PGRP2 (RbPGRP2) was identified in the intestine by next generation sequencing (NGS) analysis. The open reading frame (ORF) the RbPGRP2 cDNA (470 amino acid r ...
DOI:
10.1016/j.fsi.2018.11.025

9. Characterization of mandarin fish (Siniperca chuatsi) IL-6 and IL-6 signal transducer and the association between their SNPs and resistance to ISKNV disease

Author:
Rui-Ming Jin; He-Zhong Huang; Yu Zhou; Ying-Ying Wang; Huang-Cui Fu; Ze Li; Xiao-Zhe Fu; Ning-Qiu Li
Source:
Fish & shellfish immunology 2021 v.113 pp. 139-147
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; alleles; disease resistance; fish; hepatocytes; inflammation; interleukin-6; kidneys; loci; metabolism; quantitative polymerase chain reaction; shellfish; signal transduction; spleen; statistical analysis
Abstract:
... In fish, interleukin-6 (IL-6) is a very important immune-regulatory cytokine that plays a polyfunctional role in inflammation, metabolism, regeneration, and neural processes. IL-6 signal transducer (IL-6ST) is a specific receptor for IL-6 and expressed mainly in immune cells and hepatocytes. In this study, the complete cDNA and genomic DNA sequences of mandarin fish (Siniperca chuatsi) IL-6 and IL ...
DOI:
10.1016/j.fsi.2021.04.003

10. Chitosan and anisodamine improve the immune efficacy of inactivated infectious spleen and kidney necrosis virus vaccine in Siniperca chuatsi

Author:
Jiacheng Zhang; Xiaozhe Fu; Yanqi Zhang; Wentao Zhu; Yong Zhou; Gailing Yuan; Xiaoling Liu; Taoshan Ai; Lingbing Zeng; Jianguo Su
Source:
Fish & shellfish immunology 2019 v.89 pp. 52-60
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; acid phosphatase; blood serum; catalase; chitosan; fish; immune response; infectious diseases; liver; messenger RNA; mortality; protective effect; reverse transcriptase polymerase chain reaction; spleen; tissues; tumor necrosis factor-alpha; urea nitrogen; vaccination; vaccine adjuvants; vaccines; virus replication
Abstract:
... Siniperca chuatsi is an economically important fish in China, but infectious spleen and kidney necrosis virus (ISKNV) causes high mortality and significant economic losses. Currently, vaccination is the most promising strategy to prevent infectious diseases, while adjuvant can effectively enhance immune responses. In this study, inactivated ISKNV vaccine was prepared, then poly (I:C), chitosan, an ...
DOI:
10.1016/j.fsi.2019.03.040

11. Cladistic analysis of iridoviruses based on protein and DNA sequences

Author:
Wang, J.W.; Deng, R.Q.; Wang, X.Z.; Huang, Y.S.; Xing, K.; Feng, J.H.; He, J.G.; Long, Q.X.
Source:
Archives of virology 2003 v.148 no.11 pp. 2181-2194
ISSN:
0304-8608
Subject:
coat proteins; insect viruses; vertebrate viruses; taxonomy; phylogeny; amino acid sequences; Infectious spleen and kidney necrosis virus; nucleotide sequences
DOI:
10.1007/s00705-003-0178-6
PubMed:
14579177

12. Cloning, characterisation, and expression analysis of the cathepsin D gene from rock bream (Oplegnathus fasciatus)

Author:
Kwang-Min Choi; Sang Hee Shim; Cheul-Min An; Bo-Hye Nam; Young-Ok Kim; Ju-Won Kim; Chan-il Park
Source:
Fish & shellfish immunology 2014 v.40 no.1 pp. 253-258
ISSN:
1050-4648
Subject:
phylogeny; cathepsin D; Streptococcus iniae; Miichthys miiuy; bream; open reading frames; gene expression; amino acids; Infectious spleen and kidney necrosis virus; spleen; active sites; homeostasis; Edwardsiella tarda; tissues; pathogens; messenger RNA; complementary DNA; muscles; Oplegnathus fasciatus; cDNA libraries; kidneys; papain; liver; innate immunity; glycosylation; expressed sequence tags
Abstract:
... Cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for the maintenance of cellular homeostasis. Rock bream (Oplegnathus fasciatus) cathepsin D (RbCTSD) cDNAs were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cDNA library. The full-length RbCTSD cDNA (1644 bp) con ...
DOI:
10.1016/j.fsi.2014.07.013

13. Cloning, characterization and expression analysis of a CXCR1-like gene from mandarin fish Siniperca chuatsi

Author:
Chen, Chao; Li, Zhongsheng; Zhou, Zhicheng; Yin, Zhixin; Chan, Siu-Ming; Yu, Xiao-Qiang; Weng, Shaoping; He, Jianguo
Source:
Fish physiology and biochemistry 2009 v.35 no.3 pp. 489-499
ISSN:
0920-1742
Subject:
nucleotides; 3' untranslated regions; fish; blood; promoter regions; 5' untranslated regions; open reading frames; clones; mandarins; amino acids; Infectious spleen and kidney necrosis virus; spleen; start codon; transcription factors; complementary DNA; kidneys; liver; binding sites; gills
Abstract:
... In this study we cloned and characterized a CXCR1-like gene (mfCXCR1) from mandarin fish (Siniperca chuatsi). The full-length cDNA of mfCXCR1 is 2,173 bp and contains a 1,056 bp open reading frame (ORF) that encodes a protein of 351 amino acids. The 5' and 3' untranslated regions (UTR) are 57 and 1,080 bp in length, respectively. The coding region of the mfCXCR1 gene consists of a single exon with ...
DOI:
10.1007/s10695-008-9283-5
PubMed:
19093223

14. Cloning, characterization, and biological function analysis of the SidT2 gene from Siniperca chuatsi

Author:
Ruiwen Ren; Xiaopeng Xu; Ting Lin; Shaoping Weng; Huafang Liang; Mengli Huang; Chuanfu Dong; Yongwen Luo; Jianguo He
Source:
Developmental and comparative immunology 2011 v.35 no.6 pp. 692-701
ISSN:
0145-305X
Subject:
Frog virus 3; Infectious spleen and kidney necrosis virus; Pimephales promelas; RNA interference; Siniperca chuatsi; antiserum; double-stranded RNA; epithelial cells; fish fry; gene overexpression; genes; messenger RNA; open reading frames; reproduction; signal peptide; tissue distribution; viruses
Abstract:
... The systemic RNA interference defective protein (SID)-1 plays an important role in dsRNA uptake in cells. We identified the ScSidT2 gene from the mandarin fish (Siniperca chuatsi), which is the first-studied SID-1 homolog in fish. ScSidT2 mRNA is 3380bp long and contains a 2568-bp open reading frame that encodes an 855-amino-acid protein with an N-terminal signal peptide and ten putative transmemb ...
DOI:
10.1016/j.dci.2011.02.003

15. Co-infection of infectious spleen and kidney necrosis virus and Francisella sp. in farmed pearl gentian grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) in China - A case report

Author:
Yucong Huang; Shuanghu Cai; Jichang Jian; Guangfeng Liu; Liwen Xu
Source:
Aquaculture 2020 v.526 pp. 735409
ISSN:
0044-8486
Subject:
Francisella; Infectious spleen and kidney necrosis virus; Iridovirus; Maccullochella peelii peelii; anorexia; case studies; females; fish farms; gills; grouper; histopathology; macrophages; males; mixed infection; mortality; phylogeny; polymerase chain reaction; sequence analysis; signs and symptoms (animals and humans); splenomegaly; swimming; viruses; China
Abstract:
... From October–December 2018, a mass mortality among cultured pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatus) occurred on a private grouper farm in Zhanjiang City, Guangdong Province, China. Approximately 120 dead fish from each pond were recorded daily, and the cumulative mortality was up to 52% within 56 days. Diseased fish showed clinical signs such as lethargy, anorexia, slo ...
DOI:
10.1016/j.aquaculture.2020.735409

16. Co-infections of infectious spleen and kidney necrosis virus and Siniperca chuatsi rhabdovirus in Chinese perch (Siniperca chuatsi)

Author:
Qiang Lin; Xiaozhe Fu; Ningqiu Li; Quanyuan Wan; Wenjie Chen; Yunmao Huang; Zhibin Huang; Jun Li; Lijuan Zhao; Li Lin
Source:
Microbial pathogenesis 2017 v.111 pp. 422-430
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; brain; farmed fish; mixed infection; pathogens; tissues; vaccines; viral load; viruses
Abstract:
... In spite of the quite common co-infections of viruses in the cultured fish, most of the previous studies have just simply focused on the infection of a single pathogen. In this report, we observed that about 13% of cultured Chinese perch have been co-infected by infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV). Furthermore, Chinese perch could co-infecte ...
DOI:
10.1016/j.micpath.2017.09.002

17. Complete Genome Sequences and Pathogenicity Analysis of Two Red Sea Bream Iridoviruses Isolated from Cultured Fish in Korea

Author:
Min-A Jeong; Ye-Jin Jeong; Kwang-Il Kim
Source:
Fishes 2021 v.6 no.4 pp. -
ISSN:
2410-3888
Subject:
DNA-binding proteins; Infectious spleen and kidney necrosis virus; Lateolabrax japonicus; Oplegnathus fasciatus; Pagrus major; adenosinetriphosphatase; bream; coat proteins; etiological agents; farmed fish; genomics; membrane proteins; pathogenicity; sequence homology; Korean Peninsula
Abstract:
... In Korea, red sea bream iridovirus (RSIV), especially subtype II, has been the main causative agent of red sea bream iridoviral disease since the 1990s. Herein, we report two Korean RSIV isolates with different subtypes based on the major capsid protein and adenosine triphosphatase genes: 17SbTy (RSIV mixed subtype I/II) from Japanese seabass (Lateolabrax japonicus) and 17RbGs (RSIV subtype II) fr ...
DOI:
10.3390/fishes6040082

18. Complete genome analysis of a red seabream iridovirus (RSIV) isolated from Asian seabass (Lates calcarifer) in India

Author:
T.G. Puneeth; Pallavi Baliga; S.K. Girisha; Malathi Shekar; M.S. Nithin; T. Suresh; B.T. Naveen Kumar
Source:
Virus research 2021 v.291 pp. 198199
ISSN:
0168-1702
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Pagrus major; adenosinetriphosphatase; coat proteins; etiological agents; farmed fish; genes; mortality; phylogeny; research; sequence analysis; viruses; India
Abstract:
... Red sea bream iridovirus (RSIV) is the causative agent of the iridoviral disease with high mortality rates in cultured fish. Our laboratory reported the first case of RSIV infection in India which resulted in mass mortalities of Asian seabass, Lates calcarifer. The RSIV-LC strain isolated from infected fish was subjected to complete genome sequencing and analysis. The complete genome of RSIV-LC wa ...
DOI:
10.1016/j.virusres.2020.198199

19. Complete genome sequence and phylogenetic analysis of megalocytivirus RSIV-Ku: A natural recombination infectious spleen and kidney necrosis virus

Author:
Jing-Yi Shiu; Jiann-Ruey Hong; Chen-Chun Ku; Chiu-Ming Wen
Source:
Archives of virology 2018 v.163 no.4 pp. 1037-1042
ISSN:
0304-8608
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Pagrus major; adenosinetriphosphatase; coat proteins; genes; genotype; open reading frames; phylogeny; viruses
Abstract:
... Megalocytiviruses are classified into three genotypes, infectious spleen and kidney necrosis virus (ISKNV), red seabream virus (RSIV), and turbo reddish body iridovirus (TRBIV), based on the major capsid protein and ATPase genes. However, only a few complete genome sequences have been obtained. This paper reports the complete genome sequence and phylogenetic analysis of an RSIV-Ku strain megalocyt ...
DOI:
10.1007/s00705-017-3689-2

20. Complete genome sequences of infectious spleen and kidney necrosis virus isolated from farmed albino rainbow sharks Epalzeorhynchos frenatum in the United States

Author:
Samantha A. Koda; Kuttichantran Subramaniam; Deborah B. Pouder; Roy P. Yanong; Salvatore Frasca Jr; Vsevolod L. Popov; Thomas B. Waltzek
Source:
Virus genes 2021 v.57 no.5 pp. 448-452
ISSN:
0920-8569
Subject:
DNA; Infectious spleen and kidney necrosis virus; albino; genotype; histopathology; kidneys; spleen; transmission electron microscopy; viruses
Abstract:
... The genus Megalocytivirus includes viruses known to cause significant disease in aquacultured fish stocks. Herein, we report the complete genome sequences of two megalocytiviruses (MCVs) isolated from diseased albino rainbow sharks Epalzeorhynchos frenatum reared on farms in the United States in 2018 and 2019. Histopathological examination revealed typical megalocytivirus microscopic lesions (i.e. ...
DOI:
10.1007/s11262-021-01857-6

21. Composition and transcription of all interferon regulatory factors (IRFs), IRF1‒11 in a perciform fish, the mandarin fish, Siniperca chuatsi

Author:
Zubair Ahmed Laghari; Li Li; Shan Nan Chen; Hui Jun Huo; Bei Huang; Ying Zhou; P. Nie
Source:
Developmental and comparative immunology 2018 v.81 pp. 127-140
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture; cytokines; fish; gene expression; genes; immune response; immunomodulation; interferon regulatory factors; interferons; kidneys; pathogens; spleen; tissues
Abstract:
... Interferon regulatory factors (IRFs) are a family of mediators in various biological processes including immune modulation of interferon (IFN) and proinflammatory cytokine expression. However, the data on the complete composition of IRFs is rather limited in teleost fish. In the present study, all IRF members, i.e. IRF1‒11 with two IRF4, IRF4a and IRF4b have been characterised in an aquaculture sp ...
DOI:
10.1016/j.dci.2017.11.015

22. Current use and development of fish vaccines in China

Author:
Qingchao Wang; Wei Ji; Zhen Xu
Source:
Fish & shellfish immunology 2020 v.96 pp. 223-234
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Ctenopharyngodon idella; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Paralichthys olivaceus; Vibrio alginolyticus; antibodies; aquaculture industry; ethics; farmed fish; field experimentation; fish production; food safety; immune response; inactivated vaccines; live vaccines; markets; shellfish; sustainable development; turbot; vaccination; veterinary medicine; China
Abstract:
... In the past decades, the aquaculture industry made great progress in China, which contributes more than 70% yield of the world's farmed fish. Along with the rapid growth of fish production, increased emergence and outbreak of numbers of diseases pose harm to the aquaculture industry and food safety. From the efficient, safe, environmental and ethical aspects, vaccines is definitely the most approp ...
DOI:
10.1016/j.fsi.2019.12.010

23. Data on molecular characterisation and expression analysis of the interferon-related developmental regulator 2 (IFRD2) gene from red sea bream, Pagrus major

Author:
Kyung Ho Kim; Andre Kim; Chan-Il Park
Source:
Data in Brief 2019 v.25 pp. 104142
ISSN:
2352-3409
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; adults; amino acid sequences; brain; gastrulation; genes; heart; hematopoiesis; immune response; kidneys; liver; lungs; myoblasts; phylogeny; placenta; skeletal muscle
Abstract:
... The interferon-related developmental regulator 1 (IFRD1) protein is expected to play a role in the regulation of inflammatory responses in adult mice, since it is known to repress transcription of NF-κB in myoblasts that regenerate skeletal muscle after traumatic injury Micheli et al., 2011. The IFRD2 gene is expressed in many tissues including skeletal muscle, kidney, heart, brain, lung, placenta ...
DOI:
10.1016/j.dib.2019.104142

24. Data on molecular characterization and expression of the C-reactive protein (CRP) gene from rock bream, Oplegnathus fasciatus

Author:
Kyung Ho Kim; Do-Hyung Kim; Chan-Il Park
Source:
Data in Brief 2019 v.26 pp. 104506
ISSN:
2352-3409
Subject:
C-reactive protein; Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; bream; gene expression; genes; inflammation; innate immunity; kidneys; nucleotide sequences; phylogeny; spleen
Abstract:
... C-reactive protein (CRP) is a member of the pentraxin family and is an acute-phase response to plasma protein; its level in blood increases rapidly in response to trauma, inflammation, and infection. In the present study, we analysed the molecular characteristics of the Oplegnathus fasciatus CRP (RbCRP) gene sequence using multiple alignments and phylogenetic analyses of the deduced amino acids. I ...
DOI:
10.1016/j.dib.2019.104506

25. Data on molecular characterization and expression profiles of the NF-κB repressing factor gene in red sea bream (Pagrus major)

Author:
Kwang-Min Choi; Jee Youn Hwang; Mun-Gyeong Kwon; Ji-Min Jeong; Jung Soo Seo; Seong Don Hwang; Bo-Yeong Jee; Mu-Chan Kim; Chan-Il Park
Source:
Data in Brief 2019 v.24 pp. 103977
ISSN:
2352-3409
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; amino acids; gene expression; genes; kidneys; liver; spleen; transcription (genetics); transcription factor NF-kappa B
Abstract:
... Nuclear factor-kappaB (NF-κB) repressing factor (NKRF) specifically inhibits the transcriptional activity of NF-κB protein. The PmNKRF cDNA is composed of 757 amino acid residues. Alignment analysis revealed that the G-patch and R3H domains are conserved in different organisms. We aimed to analyse red sea bream NKRF (PmNKRF) gene expression after infection with pathogens [Streptococcus iniae or re ...
DOI:
10.1016/j.dib.2019.103977

26. Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

Author:
Min Jin Heo; Andre Kim; Chan-Il Park
Source:
Data in Brief 2019 v.25 pp. 103901
ISSN:
2352-3409
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; cytokines; gene expression; genes; immune response; macrophages; membrane proteins; monocytes; phylogeny; secretion
Abstract:
... Secretory carrier membrane proteins (SCAMPs) are widely distributed integral membrane proteins implicated in membrane trafficking. Secretory carrier membrane protein 5 (SCAMP5) is expected to be involved in regulation of the immune response because it is expressed in a variety of immune tissues and promotes the secretion of cytokines in monocytes and macrophages. In this study, we performed an ana ...
DOI:
10.1016/j.dib.2019.103901

27. Deleting ORF71L of infectious spleen and kidney necrosis virus (ISKNV) resulted in virulence attenuation in Mandarin fish

Author:
Hetong Zhang; Hemei Qi; Shaoping Weng; Jianguo He; Chuanfu Dong
Source:
Fish & shellfish immunology 2022 v.123 pp. 335-347
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture industry; fish fry; genes; homologous recombination; immunology; mortality; mutants; peptides; protein synthesis; shellfish; structural proteins; transcriptomics; viral genome; virion; virulence; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus Megalocytivirus, infects a variety of teleost fish species and causes substantial losses in the aquaculture industry worldwide. ISKNV ORF71L is 1611 bp in length, encodes a 537-amino-acid peptide and was previously identified as a viral structural protein in the ISKNV virion. In this study, the ORF71L deletion mutan ...
DOI:
10.1016/j.fsi.2022.02.041

28. Deletion of the Infectious spleen and kidney necrosis virus ORF069L reduces virulence to mandarin fish Siniperca chuatsi

Author:
Yi-Fan Lin; Jian He; Ruo-Yun Zeng; Zhi-Min Li; Zhi-Yong Luo; Wei-Qiang Pan; Shao-Ping Weng; Chang-Jun Guo; Jian-Guo He
Source:
Fish & shellfish immunology 2019 v.95 pp. 328-335
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Retroviridae; Siniperca chuatsi; T-lymphocytes; amino acids; carcinogenesis; cell proliferation; death; fish; fish diseases; fruit diseases; gene deletion; genes; growth curves; homologous recombination; humans; immunoglobulin M; interleukin-1beta; interleukin-8; live vaccines; terminal repeat sequences; viral load; virulence; viruses; China
Abstract:
... Mandarin fish (Siniperca chuatsi) is a significant cultured species with high added value in China. With the expansion of farming, diseases of mandarin fish such as Infectious spleen and kidney necrosis virus (ISKNV) diseases are becoming more and more serious. Human endogenous retrovirus subfamily H long terminal repeat associating protein 2 (HHLA2) is a type 1 transmembrane molecule with three e ...
DOI:
10.1016/j.fsi.2019.10.039

29. Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in Peninsular Malaysia

Author:
K Subramaniam; M Shariff; A R Omar; M Hair‐Bejo; B L Ong
Source:
Journal of fish diseases 2014 v.37 no.7 pp. 609-618
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; breeding; coat proteins; enzymes; freshwater; genes; genotype; nucleotide sequences; ornamental fish; phylogeny; polymerase chain reaction; sequence analysis; turbot; viruses; Malaysia
Abstract:
... ‘Gold standard’ OIE reference PCR assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (ISKNV) in freshwater ornamental fish from Malaysia. From total of 210 ornamental fish samples representing 14 species, ISKNV was detected in 36 samples representing 5 fish species. All positive cases did not show any clinical signs of ISKNV. Three restriction enzymes analyses ...
DOI:
10.1111/jfd.12152
PubMed:
23952914

30. Detection of dwarf gourami iridovirus (Infectious spleen and kidney necrosis virus) in populations of ornamental fish prior to and after importation into Australia, with the first evidence of infection in domestically farmed Platy (Xiphophorus maculatus)

Author:
Anneke E. Rimmer; Joy A. Becker; Alison Tweedie; Mark Lintermans; Matthew Landos; Fran Stephens; Richard J. Whittington
Source:
Preventive veterinary medicine 2015 v.122 no.1-2 pp. 181-194
ISSN:
0167-5877
Subject:
Infectious spleen and kidney necrosis virus; Xiphophorus maculatus; biosecurity; breeding; certification; imports; international trade; issues and policy; ornamental fish; pathogens; quantitative polymerase chain reaction; quarantine; risk; risk analysis; virulent strains; viruses; Queensland
Abstract:
... The movement of ornamental fish through international trade is a major factor for the transboundary spread of pathogens. In Australia, ornamental fish which may carry dwarf gourami iridovirus (DGIV), a strain of Infectious spleen and kidney necrosis virus (ISKNV), have been identified as a biosecurity risk despite relatively stringent import quarantine measures being applied. In order to gain know ...
DOI:
10.1016/j.prevetmed.2015.09.008

31. Detection of natural infection of infectious spleen and kidney necrosis virus in farmed tilapia by hydroxynapthol blue‐loop‐mediated isothermal amplification assay

Author:
R. Suebsing; P.J. Pradeep; S. Jitrakorn; S. Sirithammajak; J. Kampeera; W.A. Turner; V. Saksmerprome; B. Withyachumnarnkul; W. Kiatpathomchai
Source:
Journal of applied microbiology 2016 v.121 no.1 pp. 55-67
ISSN:
1364-5072
Subject:
Infectious spleen and kidney necrosis virus; Oreochromis niloticus; aquaculture industry; brain; coat proteins; color; colorimetry; control methods; experts; farm management; farmed fish; genes; hatcheries; loop-mediated isothermal amplification; microbial detection; oocytes; ovarian follicles; pathogens; polymerase chain reaction; screening; tissue tropism; viruses
Abstract:
... AIMS: Infectious spleen and kidney necrosis virus (ISKNV) has recently been recognized as a causative agent of serious systemic disease in tilapia. Our objective was to establish a new colorimetric loop‐mediated isothermal amplification (LAMP) assay with pre‐addition of hydroxynapthol blue (blue‐LAMP) to investigate ISKNV transmission in tilapia. METHODS AND RESULTS: The blue‐LAMP, targeting a maj ...
DOI:
10.1111/jam.13165

32. Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus

Author:
Qiang Lin; Xiaozhe Fu; Lihui Liu; Hongru Liang; Yinjie Niu; Yuanya Wen; Zhibin Huang; Ningqiu Li
Source:
Aquaculture 2020 v.529 pp. 735697
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; detection limit; droplets; fish; fish industry; kidneys; monitoring; necrosis; quantitative polymerase chain reaction; spleen; viral load
Abstract:
... Infectious spleen and kidney necrosis, caused by the infectious spleen and kidney necrosis virus (ISKNV), has damaged the economy of the world fish industry. Droplet digital PCR (ddPCR) is a novel, sensitive, accurate and absolute quantitation method that does not require a standard curve. In this study, we established a sensitive ddPCR method to rapidly detect and quantify ISKNV DNA. The establis ...
DOI:
10.1016/j.aquaculture.2020.735697

33. Development and application of a sensitive droplet digital PCR for the detection of red mark syndrome infection in rainbow trout (Oncorhynchus mykiss)

Author:
M. Orioles; M. Bulfoni; E. Saccà; D. Cesselli; J.G. Schmidt; M. Galeotti
Source:
Aquaculture 2022 v.551 pp. 737910
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Oncorhynchus mykiss; aquaculture; detection limit; droplets; etiology; markets; monitoring; quantitative polymerase chain reaction; spleen
Abstract:
... Red Mark Syndrome (RMS) is a skin disease of uncertain etiology affecting farmed rainbow trout (Oncorhynchus mykiss). It consists of single or multiple skin lesions usually localized on the trunk of fish approaching market size. Studies showed as rickettsia-like organism (RLO), currently referred as Midichloria-like organism (MLO), is supposed to be involved in RMS. Midichloria-like has been ident ...
DOI:
10.1016/j.aquaculture.2022.737910

34. Development of a high-dose vaccine formulation for prevention of megalocytivirus infection in rock bream (Oplegnathus fasciatus)

Author:
Woo Ju Kwon; Jae Chan Choi; Suhee Hong; Young Chul Kim; Min Gyeong Jeong; Joon Gyu Min; Joon Bum Jeong; Kwang Il Kim; Hyun Do Jeong
Source:
Vaccine 2020 v.38 no.51 pp. 8107-8115
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Pagrus major; adjuvants; aluminum hydroxide; antigens; bream; cell lines; dose response; mortality; squalene; water temperature
Abstract:
... A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivirus vaccine (Ultra HSCMV, 7.0 × 10¹⁰ copies/mL), a high-dose supernatant of cultur ...
DOI:
10.1016/j.vaccine.2020.11.001

35. Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus

Author:
Yasuhiko Kawato; Hirofumi Yamashita; Kei Yuasa; Satoshi Miwa; Kazuhiro Nakajima
Source:
Aquaculture 2017 v.473 pp. 291-298
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus; cytopathogenicity; mortality; vaccines; viral load
Abstract:
... We developed a cell line (SKF-9) from spotted knifejaw (Oplegnathus punctatus), which is highly susceptible to red sea bream iridovirus (RSIV). Cytopathic effect (CPE) characterized by rounding and enlargement was clearly observed in RSIV-infected SKF-9 cells. The amount of viral production of SKF-9 cells was approximately 100 times greater than that of GF cells, which is conventionally used for R ...
DOI:
10.1016/j.aquaculture.2017.02.027

36. Development of a quantitative polymerase chain reaction (qPCR) assay for the detection of dwarf gourami iridovirus (DGIV) and other megalocytiviruses and comparison with the Office International des Epizooties (OIE) reference PCR protocol

Author:
Anneke E. Rimmer; Joy A. Becker; Alison Tweedie; Richard J. Whittington
Source:
Aquaculture 2012 v.358-359 no. pp. 155-163
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; detection limit; fish diseases; ornamental fish; pathogens; polymerase chain reaction; quantitative analysis; quarantine; reference standards; screening; viruses; Australia
Abstract:
... Viruses in the genus Megalocytivirus (Family Iridoviridae) have emerged as a source of significant disease and mortality in fish worldwide. Although regarded as exotic in Australia, the importation of ornamental fish provides a transmission pathway for the introduction of a megalocytivirus, dwarf gourami iridovirus (DGIV), which is a strain of infectious spleen and kidney necrosis virus (ISKNV). T ...
DOI:
10.1016/j.aquaculture.2012.06.034

37. Development of cross‐priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

Author:
Terawut Prasitporn; Saengchan Senapin; Akapon Vaniksampanna; Siwaporn Longyant; Parin Chaivisuthangkura
Source:
Journal of fish diseases 2021 v.44 no.9 pp. 1411-1422
ISSN:
0140-7775
Subject:
DNA; Infectious spleen and kidney necrosis virus; Lates calcarifer; Redspotted grouper nervous necrosis virus; coat proteins; colorimetry; cross reaction; fish; genes; temperature; uracil-DNA glycosylase; viruses
Abstract:
... Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil‐DNA glycosylase (UDG)‐supplemented cross‐priming amplification (UCPA) combined with a colorimetric detection method u ...
DOI:
10.1111/jfd.13448

38. Development of red sea bream iridovirus concentration method in seawater by iron flocculation

Author:
Yasuhiko Kawato; Takafumi Ito; Takashi Kamaishi; Atushi Fujiwara; Mitsuru Ototake; Toshihiro Nakai; Kazuhiro Nakajima
Source:
Aquaculture 2016 v.450 pp. 308-312
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Seriola quinqueradiata; aquaculture; detection limit; fish diseases; flocculation; genome; monitoring; mortality; pathogens; prediction; quantitative analysis; quantitative polymerase chain reaction; rearing; seawater; viruses; Japan
Abstract:
... Pathogen dynamics in environmental water are considered to be highly associated with the prevalence of fish disease. To examine the dynamics of red sea bream iridovirus (RSIV), which has caused serious damage in marine aquacultures in Japan, we developed a highly sensitive and quantitative method by combining the viral concentration and real-time PCR to detect the virus in seawater. The iron flocc ...
DOI:
10.1016/j.aquaculture.2015.08.016

39. Development of single nucleotide polymorphism (SNP) application for detection and genotyping of RSIV‐type megalocytiviruses

Author:
Channapha Sakseepipad; Reiko Nozaki; Keisuke Yoshii; Yutaka Fukuda; Kaori Mizuno; Hidemasa Kawakami; Ikuo Hirono; Hidehiro Kondo
Source:
Journal of fish diseases 2021 v.44 no.9 pp. 1337-1342
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Japan; genome; genotyping; haplotypes; mortality; phylogeny; single nucleotide polymorphism; viruses
Abstract:
... Red sea bream iridovirus (RSIV) belonging to the genus Megalocytivirus of the family Iridoviridae is the cause of serious mass mortality of cultured marine fishes. RSIV‐type megalocytiviruses show extremely high nucleotide sequence identities. Thus, epidemiological studies on this virus are limited. This study developed two primer sets amplifying the regions possessing single nucleotide polymorphi ...
DOI:
10.1111/jfd.13392

40. Development of two cell lines from yellowfin seabream Acanthopagrus latus fin and brain suitable for propagating SDDV but not for ISKNV, MRV and GIV

Author:
Yuting Fu; Yong Li; Xiangrong Liu; Weixuan Fu; Shaoping Weng; Fangzhao Yu; Jianguo He; Chuanfu Dong
Source:
Aquaculture 2022 v.560 pp. 738478
ISSN:
0044-8486
Subject:
Acanthopagrus; Infectious spleen and kidney necrosis virus; Iridovirus; Ranavirus; Western blotting; aquaculture; ascites; brain; cell culture; cell lines; chromosomes; cytopathogenicity; etiological agents; fetal bovine serum; fluorescent antibody technique; grouper; karyotyping; quantitative polymerase chain reaction; transmission electron microscopy; vaccines; viruses; China
Abstract:
... Scale drop disease virus (SDDV), a very distinct member of genus Megalocytivirus in family Iridoviridae, was recently confirmed as the most alarming causative agent associated with yellowfin seabream Acanthopagrus latus ascites diseases in South China. To better understand how host-pathogen interacting and developing cell-culture based vaccine, establishment of highly susceptible cell line generat ...
DOI:
10.1016/j.aquaculture.2022.738478

41. Differential autophagic effects triggered by five different vertebrate iridoviruses in a common, highly permissive mandarinfish fry (MFF-1) cell model

Author:
Hemei Qi; Yang Yi; Shaoping Weng; Weibing Zou; Jianguo He; Chuanfu Dong
Source:
Fish & shellfish immunology 2016 v.49 pp. 407-419
ISSN:
1050-4648
Subject:
Frog virus 3; Infectious spleen and kidney necrosis virus; Iridovirus; Santee-Cooper ranavirus; Western blotting; autophagy; fluorescence; host-pathogen relationships; models; salamanders and newts; transmission electron microscopy
Abstract:
... Autophagy of five vertebrate iridoviruses, including one megalocytivirus (infectious spleen and kidney necrosis virus, ISKNV) and four ranaviruses (Chinese giant salamander iridovirus, CGSIV; Tiger frog virus, TFV; Grouper iridovirus, GIV; and Largemouth bass virus, LMBV) were investigated in a common, highly permissive mandarinfish fry (MFF-1) cell model. The results showed marked autophagosome f ...
DOI:
10.1016/j.fsi.2015.12.041

42. Disease resistance and immune‐relevant gene expression in golden mandarin fish, Siniperca scherzeri Steindachner, infected with infectious spleen and kidney necrosis virus‐like agent

Author:
G W Shin; S L White; H U Dahms; H D Jeong; J H Kim
Source:
Journal of fish diseases 2014 v.37 no.12 pp. 1041-1054
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Siniperca scherzeri; disease resistance; financial economics; fish; gene expression; genes; interleukin-8; kidneys; messenger RNA; mortality; pathogenicity; quantitative polymerase chain reaction; spleen
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), family Iridoviridae, genus Megalocytivirus, may cause high mortality rates such as those seen in mandarin fish, Siniperca chuatsi. ISKNV has attracted much attention due to the possible environmental threat and economic losses it poses on both cultured and wild populations. We have investigated the pathogenicity of ISKNV‐like agent Megalocytivir ...
DOI:
10.1111/jfd.12182

43. Display of ISKNV orf086 protein on the surface of Aeromonas hydrophila and its immunogenicity in Chinese perch (Siniperca chuatsi)

Author:
Xiaozhe Fu; Qiang Lin; Lihui Liu; Hongru Liang; Zhibin Huang; Ningqiu Li
Source:
Fish & shellfish immunology 2016 v.56 pp. 286-293
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; adjuvants; antibodies; aquaculture industry; bacteria; enzyme-linked immunosorbent assay; fish; fluorescent antibody technique; formalin; gene expression regulation; genes; homologous recombination; immune response; immunoglobulin M; interferon regulatory factor-7; lymphocyte proliferation; lysozyme; mixed infection; mortality; mutants; reverse transcriptase polymerase chain reaction; surface proteins; transcription (genetics); vaccines; viral antigens
Abstract:
... Co-infection with infectious spleen and kidney necrosis virus (ISKNV) and Aeromonas hydrophila is becoming ever more widespread in Chinese perch (Siniperca chuatsi) aquaculture industry, so that it’s necessary to develop the combined vaccine against ISKNV and A. hydrophila disease. The surface display of heterologous on bacteria using anchoring motifs from outer membranes proteins has already been ...
DOI:
10.1016/j.fsi.2016.07.023

44. EPA and DHA can modulate cell death via inhibition of the Fas/tBid-mediated signaling pathway with ISKNV infection in grouper fin cell line (GF-1) cells

Author:
Kuang-Wen Chen; Hsuan-Wen Chiu; Yu-Wei Chiu; Jen-Leih Wu; Jiann-Ruey Hong
Source:
Fish & shellfish immunology 2020 v.97 pp. 608-616
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; apoptosis; caspase-3; caspase-8; cell lines; cell membranes; cell viability; death; docosahexaenoic acid; eicosapentaenoic acid; fish diseases; gene expression regulation; genes; grouper; nucleotidyltransferases; signal transduction; viral load
Abstract:
... Polyunsaturated fatty acids (PUFAs) play important roles in organisms, including the structure and liquidity of cell membranes, anti-oxidation and anti-inflammation. Very little has been done in terms of the effect of PUFAs on cell death, especially on DNA virus. In this study, we demonstrated that the infectious spleen and kidney necrosis virus (ISKNV) can induce host cell death via the apoptotic ...
DOI:
10.1016/j.fsi.2019.10.029

45. Early protein ORF086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish Siniperca chuatsi

Author:
Xiaozhe Fu; Ningqiu Li; Qiang Lin; Huizhi Guo; Lihui Liu; Zhibin Huang; Shuqin Wu
Source:
Fish & shellfish immunology 2015 v.46 no.2 pp. 200-205
ISSN:
1050-4648
Subject:
Escherichia coli; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; adjuvants; antigens; aquaculture industry; fish; gene expression regulation; genes; immunoglobulin M; interferon regulatory factor-7; mortality; muscle tissues; polyclonal antibodies; rabbits; transcription (genetics); vaccination; vaccines
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) has caused significant loss in the Mandarin fish (Siniperca chuatsi) aquaculture industry. Vaccination is an important measure to prevent fatal ISKNV infection. In this study, the ORF086 gene encoding an early protein helicase of ISKNV was cloned into the prokaryotic pET32a (+) and eukaryotic pcDNA3.1 (+) expression vectors and designated as pET0 ...
DOI:
10.1016/j.fsi.2015.05.052

46. Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins

Author:
Chuanfu Dong; Xiaopeng Xiong; Yongwen Luo; Shaoping Weng; Qing Wang; Jianguo He
Source:
Veterinary microbiology 2013 v.162 no.2-4 pp. 419-428
ISSN:
0378-1135
Subject:
Infectious spleen and kidney necrosis virus; Western blotting; antibodies; coat proteins; control methods; etiological agents; fish; fish industry; fluorescent antibody technique; immunity; immunoglobulin M; mass spectrometry; neutralization; recombinant proteins; two-dimensional gel electrophoresis; vaccines; virion; viruses; China
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), the type species of genus Megalocytivirus in the family Iridoviridae, is the most important etiological agents in mandarin fish industry in China. Since its first occurrence in China in the early 1990s, there is no effective method to prevent and control this virus. Here we report the successful development of a formalin-killed cell-cultured (FK ...
DOI:
10.1016/j.vetmic.2012.10.026
PubMed:
23177910

47. Establishment and characterization of a permanent heart cell line from largemouth bass Micropterus salmoides and its application to fish virology and immunology

Author:
Weiwei Zeng; Hanxu Dong; Xiaoyu Chen; Sven M. Bergmann; Ying Yang; Xinxian Wei; Guixiang Tong; Hua Li; Hui Yu; Yanfeng Chen
Source:
Aquaculture 2022 v.547 pp. 737427
ISSN:
0044-8486
Subject:
Betanodavirus; Carp sprivivirus; Ctenopharyngodon idella; Infectious spleen and kidney necrosis virus; Micropterus salmoides; Mycoplasma; Ranavirus; Reoviridae; Tilapia lake virus; aquaculture; cell lines; cytochrome-c oxidase; diploidy; electron microscopy; farmed fish; fetal bovine serum; gene expression; genotype; heart; industry; interferons; karyotyping; reporter genes; virology; China
Abstract:
... Largemouth bass (Micropterus salmoides) is among the most important cultured fish species in China and various viral diseases have greatly affected the largemouth bass industry. We established a permanent cell line from the heart of M. salmoides (MSH) that has been subcultured over 70 times and showed optimal growth at 27 °C using Medium 199 supplemented with 10% fetal bovine serum. MSH cells cons ...
DOI:
10.1016/j.aquaculture.2021.737427

48. Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Author:
Jinpeng Gong; Xiaoyi Pan; Lingyun Lin; Yue Zhu; Jiayun Yao; Congxu Wang; Wenlin Yin; Lei Huang; Yihan Liu; Fan Chen; Jinyu Shen
Source:
Journal of fish diseases 2022 v.45 no.10 pp. 1419-1427
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Ranavirus; Rhabdoviridae; Siniperca chuatsi; cell lines; cytochrome-c oxidase; diploidy; fetal bovine serum; fish; karyotyping; spinal cord; transmission electron microscopy
Abstract:
... In this study, we established and characterized a continuous cell line from the spinal cord tissue of mandarin fish, Siniperca chuatsi and assessed its susceptibility to infectious spleen and kidney necrosis virus (ISKNV), Siniperca chuatsi ranavirus (SCRaV) and Siniperca chuatsi rhabdovirus (SCRV). The cell line, named SCC, has been successively cultured up to 40 passages. The optimal growing con ...
DOI:
10.1111/jfd.13671

49. First description of a natural infection with spleen and kidney necrosis virus in zebrafish

Author:
Roberto Bermúdez; Ana Paula Losada; Ana Manuela de Azevedo; Jorge Guerra‐Varela; David Pérez‐Fernández; Laura Sánchez; Francesc Padrós; Barbara Nowak; María Isabel Quiroga
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1283-1294
ISSN:
0140-7775
Subject:
Danio rerio; Infectious spleen and kidney necrosis virus; anorexia; biosecurity; distress; farmed fish; fins; gills; hemorrhage; histopathology; kidneys; models; morbidity; necrosis; pathogenesis; phylogeny; research facilities; spleen; swimming; tissues; viruses; Spain
Abstract:
... Zebrafish has become a popular research model in the last years, and several diseases affecting zebrafish research facilities have been reported. However, only one case of naturally occurring viral infections was described for this species. In 2015, infectious spleen and kidney necrosis virus (ISKNV) was detected in zebrafish from a research facility in Spain. Affected fish showed lethargy, loss o ...
DOI:
10.1111/jfd.12822

50. First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens

Author:
Ju-Won Kim; Ji-Min Jeong; Jin-Sol Bae; Dong-Hee Cho; Sung Hee Jung; Jee-Youn Hwang; Mun-Gyeong Kwon; Jung Soo Seo; Gun-Wook Baeck; Chan-Il Park
Source:
Developmental and comparative immunology 2016 v.55 pp. 51-55
ISSN:
0145-305X
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; amino acids; apoptosis; bream; complementary DNA; erythrocytes; gene expression; gills; immune response; intestines; kidneys; liver; mortality; open reading frames; pathogens; spleen
Abstract:
... In this study, we isolated and characterized programmed cell death10 (PDCD10), which is known to be related to apoptosis, from rock bream (Oplegnathus fasciatus). The full-length rock bream PDCD10 (RbPDCD10) cDNA (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. Furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among th ...
DOI:
10.1016/j.dci.2015.10.009