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- Escobar, C.; Barcala, M.; Portillo, M.; Almoguera, C.; Jordano, J.; Fenoll, C.
- Molecular plant-microbe interactions 2003 v.16 no.12 pp. 1062-1068
- Nicotiana tabacum; tobacco; transgenic plants; Helianthus annuus; promoter regions; heat shock proteins; recombinant DNA; reporter genes; beta-glucuronidase; gene induction; Meloidogyne incognita; root-knot nematodes; giant cells; root galls; roots; point mutation
- ... Root-knot nematodes feed from specialized giant cells induced in the plants that they parasitize. We found that the promoter of the Hahsp17.7G4 gene, which encodes a small heat-shock protein involved in embryogenesis and stress responses, directed GUS expression in tobacco galls induced by the root-knot nematode Meloidogyne incognita. In roots containing a GUS reporter fusion to the Hahsp17.7G4 pr ...
- Almoguera, C.; Prieto-Dapens, P.; Jordano, J.
- plant journal 1998 v.13 no.4 pp. 437-446
- heat shock proteins; plant development; Helianthus annuus; seeds; transgenic plants; enzyme activity; mutation; gene expression; histochemistry; Nicotiana tabacum; reporter genes; binding sites; embryogenesis; promoter regions; beta-glucuronidase
- ... Transgenic tobacco expression was analysed of chimeric genes with point mutations in the heat shock element (HSE) arrays of small heat shock protein (sHSP) gene from sunflower hp17.7 G4. The promotor was developmentally regulated during zygotic and responded to heat stress in vegetative tissues. Mutations in the HSE affected nucleotides crucial for human heat shock transcription factor 1 (HSF1) bi ...
- Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
- Plant physiology 1997 v.114 no.2 pp. 419-428
- Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
- ... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...