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You searched for: Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase Subject term jasmonic acid Remove constraint Subject term: jasmonic acid
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1. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

2. Regulation of sesquiterpene cyclase gene expression. Characterization of an elicitor- and pathogen-inducible promoter

Author:
Yin, S.; Mei, L.; Newman, J.; Back, K.; Chappell, J.
Source:
Plant physiology 1997 v.115 no.2 pp. 437-451
ISSN:
0032-0889
Subject:
sesquiterpenoids; plant pathogenic fungi; salicylic acid; promoter regions; jasmonic acid; hydrogen peroxide; gene expression; Nicotiana tabacum; histology; transcription (genetics); pathogenicity; Phytophthora nicotianae var. parasitica; enzyme activity; genetic transformation; gene transfer; biosynthesis; plant anatomy; transgenic plants; Pseudomonas syringae pv. syringae; reporter genes; beta-glucuronidase
Abstract:
... The promoter for a tobacco (Nicotiana tabacum) sesquiterpene cyclase gene, a key regulatory step in sesquiterpene phytoalexin biosynthesis, has been analyzed. The EAS4 promoter was fused to the beta-glucuronidase (GUS) reporter gene, and the temporal and spatial expression patterns of GUS activity were examined in stably transformed plants and in transient expression assays using electroporated pr ...
PubMed:
9342864
PubMed Central:
PMC158501

3. Activation of hsr203, a plant gene expressed during incompatible plant-pathogen interactions, is correlated with programmed cell death

Author:
Pontier, D.; Tronchet, M.; Rogowsky, P.; Lam, E.; Roby, D.
Source:
Molecular plant-microbe interactions 1998 v.11 no.6 pp. 544-554
ISSN:
0894-0282
Subject:
Nicotiana tabacum; Ralstonia solanacearum; plant pathogenic bacteria; gene expression; defense mechanisms; transgenic plants; beta-glucuronidase; reporter genes; complementary DNA; Solanum lycopersicum var. lycopersicum; hydrogen peroxide; leaves; salicylic acid; jasmonic acid; niacin; necrosis; heavy metals; apoptosis; genetic markers; enzyme activity; nucleotide sequences; amino acid sequences; hypersensitive response; histochemistry
Abstract:
... hsr203J is a tobacco gene whose activation is rapid, highly localized, and specific for incompatible interactions between tobacco and the bacterial pathogen Ralstonia solanacearum. The effect of other hypersensitive response (HR)-inducing pathogens and elicitors has been tested with transgenic plants containing the hsr203J promoter-GUS reporter gene fusion, and confirms the generality of the prefe ...
DOI:
10.1094/MPMI.1998.11.6.544
PubMed:
9612953

4. Immediate early induction of mRNAs for ethylene‐responsive transcription factors in tobacco leaf strips after cutting

Author:
Kaoru Suzuki; Nao Suzuki; Masaru Ohme‐Takagi; Hideaki Shinshi
Source:
plant journal 1998 v.15 no.5 pp. 657-665
ISSN:
0960-7412
Subject:
transcription factors; ethylene; messenger RNA; protein synthesis; cycloheximide; transgenic plants; jasmonic acid; cutting; methyl jasmonate; biochemical pathways; gene expression; Nicotiana tabacum; reporter genes; mechanical damage; promoter regions; beta-glucuronidase; leaves; defense mechanisms
Abstract:
... To investigate the functional relationship between the expression of genes for ethylene‐responsive transcription factors (ERFs) and the expression of ethylene‐responsive genes, we examined the expression of genes for ERFs and the expression of a reporter gene in transgenic tobacco that carried a gene for β‐glucuronidase (GUS) under the control of the ethylene‐responsive element, which includes fou ...
DOI:
10.1046/j.1365-313x.1998.00243.x

5. Structure and regulation of the Arabidopsis thaliana allene oxide synthase gene

Author:
Kubigsteltig, I.; Laudert, D.; Weiler, E.W.
Source:
Planta 1999 v.208 no.4 pp. 463-471
ISSN:
0032-0935
Subject:
allene; flowering; dienoic fatty acids; plant organs; pollen; promoter regions; jasmonic acid; gene expression; Nicotiana tabacum; mechanical damage; petioles; introns; Arabidopsis thaliana; abscission; ligases; enzyme activity; histochemistry; transgenic plants; reporter genes; beta-glucuronidase
Abstract:
... Allene oxide synthase (AOS) is encoded by a single intronless gene in Arabidopsis thaliana (L.) Heynh. The promoter region of the AOS gene exhibits, in addition to the elements of a minimal promoter and the presence of general enhancers, cis-elements that, in other promoters, are responsible for stress- and ethylene-responsiveness. Arabidopsis thaliana and Nicotiana tabacum L. were transformed wit ...
DOI:
10.1007/s004250050583
PubMed:
10420644

6. Wound-induced expression of the FAD7 gene is mediated by different regulatory domains of its promoter in leaves/stems and roots

Author:
Nishiuchi, T.; Kodama, H.; Yanagisawa, S.; Iba, K.
Source:
Plant physiology 1999 v.121 no.4 pp. 1239-1246
ISSN:
0032-0889
Subject:
promoter regions; stems; jasmonic acid; transgenic plants; biochemical pathways; gene expression; transcription (genetics); Nicotiana tabacum; mechanical damage; reporter genes; roots; beta-glucuronidase; leaves
Abstract:
... The FAD7 gene is expressed preferentially in the chlorophyllous tissues of unwounded plants. Wounding activates the expression of the FAD7 gene not only in chlorophyllous tissues, but also in nonchlorophyllous tissues of stems and roots. Our previous study suggested that wound-responsive transcriptional activation by the FAD7 promoter in leaves/stems and roots is brought about by a jasmonic acid ( ...
PubMed:
10594110
PubMed Central:
PMC59490

7. Expression of a pathogen-induced gene can be mimicked by auxin insensitivity

Author:
Mayda, E.; Marques, C.; Conejero, V.; Vera, P.
Source:
Molecular plant-microbe interactions 2000 v.13 no.1 pp. 23-31
ISSN:
0894-0282
Subject:
Solanum lycopersicum var. lycopersicum; Nicotiana tabacum; Arabidopsis thaliana; gene expression; auxins; defense mechanisms; infection; peroxidases; salicylic acid; ethylene; jasmonic acid; biochemical pathways; leaves; mechanical damage; transgenic plants; beta-glucuronidase; reporter genes; mutants; homeostasis; Tomato mosaic virus; nucleotide sequences
Abstract:
... Following perception of a pathogenic attack, plants are able to develop a strong response with the corresponding activation of a plethora of defense-related genes. In this study we have characterized the mode of expression of the CEVI-1 gene from tomato plants, which encodes an anionic peroxidase. CEVI-1 expression is induced during the course of compatible viral and subviral infections, like many ...
DOI:
10.1094/MPMI.2000.13.1.23
PubMed:
10656582

8. CYP82E4-mediated nicotine to nornicotine conversion in tobacco is regulated by a senescence-specific signaling pathway

Author:
Chakrabarti, Manohar; Bowen, Steven W.; Coleman, Nicholas P.; Meekins, Karen M.; Dewey, Ralph E.; Siminszky, Balazs
Source:
Plant molecular biology 2008 v.66 no.4 pp. 415-427
ISSN:
0167-4412
Subject:
ethylene; yeast extract; drought; genes; salicylic acid; promoter regions; plant damage; roots; Tobacco mosaic virus; stems; nicotine; jasmonic acid; gene expression; mature plants; Nicotiana tabacum; gene expression regulation; leaves; fluorometry; nornicotine; transcription (genetics); cytochrome P-450; enzyme activity; tobacco; loci; flowers; abscisic acid; beta-glucuronidase
Abstract:
... Nicotine to nornicotine conversion in tobacco (Nicotiana tabacum L.) is regulated by an unstable converter locus which in its activated state gives rise to a high nornicotine, low nicotine phenotype in the senescing leaves. In plants that carry the high nornicotine trait, nicotine conversion is primarily catalyzed by a cytochrome P450 protein, designated CYP82E4 whose transcription is strongly upr ...
DOI:
10.1007/s11103-007-9280-6
PubMed:
18196465

9. Functional Analysis of the Promoter of a Glycosyl Hydrolase Gene Induced in Resistant Sinapis alba by Alternaria brassicicola

Author:
Chatterjee Madhuvanti; Mazumder Mrinmoy; Basu Debabrata
Source:
Phytopathology 2013 v.103 no.8 pp. 841-850
ISSN:
0031-949X
Subject:
Alternaria brassicicola; Brassica juncea; Nicotiana tabacum; Sinapis alba; abscisic acid; adverse effects; beta-glucuronidase; gene expression; gene expression regulation; genes; genetically modified organisms; jasmonic acid; salicylic acid
Abstract:
... A putative family 3 glycosyl hydrolase (GH) gene showed significant differential expression in resistant Sinapis alba, compared with the susceptible Brassica juncea, as part of the initial responses during interaction with the necrotroph Alternaria brassicicola. To understand the mechanism of induction, the promoter was isolated and deletion analysis carried out. All the promoter fragments were fu ...
DOI:
10.1094/PHYTO-11-12-0303-R

10. Qualitative and quantitative analysis of the Nictaba promoter activity during development in Nicotiana tabacum

Author:
Annelies Delporte; Sofie Van Holle; Els J.M. Van Damme
Source:
Plant physiology and biochemistry 2013 v.67 pp. 162-168
ISSN:
0981-9428
Subject:
Arabidopsis thaliana; Nicotiana tabacum; beta-glucuronidase; cotyledons; fluorometry; jasmonic acid; leaves; lectins; quantitative analysis; reporter genes; roots; staining; tobacco; transgenic plants
Abstract:
... Nictaba is a genuine jasmonate inducible lectin expressed in the leaves and roots of Nicotiana tabacum cv. Samsun NN. Although the jasmonate pathway is generally highly conserved among plant species, recent research showed that it is followed by plant specific downstream processes. Previously the Nictaba promoter activity was studied in the model plant Arabidopsis thaliana, using the β-glucuronida ...
DOI:
10.1016/j.plaphy.2013.03.005

11. Promoters of HcTPS1 and HcTPS2 Genes from Hedychium coronarium Direct Floral-Specific, Developmental-Regulated and Stress-Inducible Gene Expression in Transgenic Tobacco

Author:
Xin-Yue Li; Shao-Yuan Zheng; Rang-Cai Yu; Yan-Ping Fan
Source:
Plant molecular biology reporter 2014 v.32 no.4 pp. 864-880
ISSN:
0735-9640
Subject:
Hedychium coronarium; Nicotiana tabacum; abiotic stress; beta-glucuronidase; breeding; flowering; fluorescence; gene expression; horticultural crops; insects; jasmonic acid; methyl jasmonate; odors; ornamental horticulture; plant hormones; plant stress; promoter regions; reporter genes; stress tolerance; transgenic plants
Abstract:
... HcTPS1 and HcTPS2 are floral-scent genes that mediate terpene formation in Hedychium coronarium. Transcription of these genes is induced by various abiotic stresses and hormonal stimuli. In this study, the regulatory mechanisms responsible for HcTPS1 and HcTPS2 expression were investigated by analyzing their promoters. Two genomic fragments, 1,298-bp and 1,928-bp in length, upstream of HcTPS1 and ...
DOI:
10.1007/s11105-013-0697-6

12. Whitefly and aphid inducible promoters of Arabidopsis thaliana L.

Author:
Neeraj Kumar Dubey; Devesh Kumar Mishra; Asif Idris; Deepti Nigam; Pradhyumna Kumar Singh; Samir V. Sawant
Source:
Journal of genetics 2018 v.97 no.1 pp. 109-119
ISSN:
0022-1333
Subject:
1-aminocyclopropane-1-carboxylic acid; Aleyrodidae; Aphidoidea; Arabidopsis thaliana; Nicotiana tabacum; abscisic acid; beta-glucuronidase; databases; genetic vectors; information sources; jasmonic acid; microarray technology; promoter regions; proteins; quantitative polymerase chain reaction; reporter genes; reverse transcriptase polymerase chain reaction; salicylic acid; tobacco; transcription (genetics); transgenic plants
Abstract:
... Lack of regulated expression and tissue specificity are the major drawbacks of plant and virus-derived constitutive promoters. A precise tissue or site-specific expression, facilitate regulated expression of proteins at the targeted time and site. Publically available microarray data on whitefly and aphid infested Arabidopsis thaliana L. was used to identify whitefly and aphid-inducible genes. The ...
DOI:
10.1007/s12041-018-0887-y