U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum Subject enzyme activity Remove constraint Subject: enzyme activity Subject measurement Remove constraint Subject: measurement Subject transgenic plants Remove constraint Subject: transgenic plants Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
Start Over

Search

8  Search Results

1 - 8 of 8
Number of results to display per page

Search Results

1. Modified ELISA for the detection of neomycin phosphotransferase II in transformed plant species

Author:
McKenzie, M.J.; Mett, V.; Jameson, P.E.
Source:
Plant cell reports 2000 v.19 no.3 pp. 286-289
ISSN:
0721-7714
Subject:
Trifolium repens; Lactuca sativa; Solanum tuberosum; micropropagation; neomycin; measurement; in vitro culture; gene expression; enzyme-linked immunosorbent assay; Nicotiana tabacum; Arabidopsis thaliana; Petunia axillaris; phosphotransferases (phosphomutases); enzyme activity; culture media; genetic transformation; gene transfer; kanamycin; Petunia hybrida; Fragaria ananassa; transgenic plants; Eustoma exaltatum subsp. russellianum; Pelargonium; genetic variation
Abstract:
... Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the use of radioisotopes. This report describes an ELISA for the detection of the neomyc ...
DOI:
10.1007/s002990050014

2. Expression of tobacco carbonic anhydrase in the C4 dicot Flaveria bidentis leads to increased leakiness of the bundle sheath and a defective CO2-concentrating mechanism

Author:
Ludwig, M.; Von Caemmerer, S.; Price, G.D.; Badger, M.R.; Furbank, R.T.
Source:
Plant physiology 1998 v.117 no.3 pp. 1071-1081
ISSN:
0032-0889
Subject:
Flaveria bidentis; genetic transformation; Nicotiana tabacum; carbonate dehydratase; genes; gene transfer; transgenic plants; gene expression; enzyme activity; leaves; vascular bundles; cytosol; cell lines; genetic variation; photosynthesis; net assimilation rate; measurement; chlorophyll; fluorescence; biochemical pathways; chemical reactions; gas exchange; promoter regions
Abstract:
... Flaveria bidentis (L.) Kuntze, a C4 dicot, was genetically transformed with a construct encoding the mature form of tobacco (Nicotiana tabacum L.) carbonic anhydrase (CA) under the control of a strong constitutive promoter. Expression of the tobacco CA was detected in transformant whole-leaf and bundle-sheath cell (bsc) extracts by immunoblot analysis. Whole-leaf extracts from two CA-transformed l ...

3. Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L

Author:
Sheveleva, E.; Chmara, W.; Bohnert, H.J.; Jensen, R.G.
Source:
Plant physiology 1997 v.115 no.3 pp. 1211-1219
ISSN:
0032-0889
Subject:
chemical constituents of plants; drought tolerance; drought; measurement; metabolites; glucose; proline; Nicotiana tabacum; fructose; photosynthesis; quantitative analysis; complementary DNA; sucrose; myo-inositol; net assimilation rate; enzyme activity; salt tolerance; salinity; transgenic plants; stress response; diurnal variation; genetic variation; stomata; transferases; leaf conductance
Abstract:
... A cDNA encoding myo-inositol O-methyltransferase (IMT1) has been transferred into Nicotiana tabacum cultivar SR1. During drought and salt stress, transformants (I5A) accumulated the methylated inositol D-ononitol in amounts exceeding 35 micromoles g-1 fresh weight In I5A, photosynthetic CO2 fixation was inhibited less during salt stress and drought, and the plants recovered faster than wild type. ...

4. The effects of salicylic acid and tobacco mosaic virus infection on the alternative oxidase of tobacco

Author:
Lennon, A.M.; Neuenschwander, U.H.; Ribas-Carbo, M.; Giles, L.; Ryals, J.A.; Siedow, J.N.
Source:
Plant physiology 1997 v.115 no.2 pp. 783-791
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell suspension culture; mitochondria; Tobacco mosaic virus; pathogenicity; disease resistance; salicylic acid; dose response; enzyme activity; oxygenases; pathogenesis; messenger RNA; measurement; cytochromes; transgenic plants; gene expression; electron transfer; systemic acquired resistance
Abstract:
... Salicylic acid (SA) is a signal in systemic acquired resistance and an inducer of the alternative oxidase protein in tobacco (Nicotiana tabacum cv Xanthi nc) cell suspensions and during thermogenesis in aroid spadices. The effects of SA on the levels of alternative oxidase protein and the pathogenesis-related 1a mRNA (a marker for systemic acquired resistance), and on the partitioning of electrons ...

5. Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli

Author:
Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 291-294
ISSN:
0721-7714
Subject:
Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
Abstract:
... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
DOI:
10.1007/BF01088283

6. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

7. Overproduction of ascorbate peroxidase in the tobacco chloroplast does not provide protection against ozone

Author:
Torsethaugen, G.; Pitcher, L.H.; Zilinskas, B.A.; Pell, E.J.
Source:
Plant physiology 1997 v.114 no.2 pp. 529-537
ISSN:
0032-0889
Subject:
genetic transformation; gene transfer; biosynthesis; phytotoxicity; gas exchange; transgenic plants; Agrobacterium radiobacter; stress response; ozone; measurement; Nicotiana tabacum; enzyme activity; peroxidase; antioxidants; chloroplasts; ascorbic acid
Abstract:
... Transgenic tobacco (Nicotiana tabacum cv Bel W3) plants were used to test the hypothesis that protection from O3 injury could be conferred by overproduction of ascorbate peroxidase (APX) in the chloroplast. The 10-fold increase in soluble APX activity in the chloroplast was expected to alleviate an implied increase in oxidative potential and prevent damage caused by O3. Three different O3 exposure ...

8. Transgenic tobacco plants expressing pea chloroplast Nmdh c DNA in sense and antisense orientation. Effects on NADP-malate dehydrogenase level, stability of transformants, and plant growth

Author:
Faske, M.; Backhausen, J.E.; Sendker, M.; Singer-Bayrle, M.; Scheibe, R.; Schaewen, A. von.
Source:
Plant physiology 1997 v.115 no.2 pp. 705-715
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Pisum sativum; leaves; chloroplasts; genes; gene transfer; genetic code; complementary DNA; antisense DNA; malic acid; alcohol oxidoreductases; transgenic plants; gene expression; measurement; enzyme activity; genetic variation; plant development; genotype; NAD (coenzyme)
Abstract:
... A full-length cDNA encoding light-activated chloroplast NADP-malate dehydrogenase (NADP-MDH) (EC 1.1.1.82) from pea (Pisum sativum L.) was introduced in the sense and antisense orientation into tobacco (Nicotiana tabacum L.). Transgenic plants with decreased or increased expression levels were obtained. Because of substantial age-dependent differences in individual leaves of a single plant, standa ...