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You searched for: Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum Subject term dose response Remove constraint Subject term: dose response
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1. In vitro effect of zinc oxide nanoparticles on Nicotiana tabacum callus compared to ZnO micro particles and zinc sulfate (ZnSO4)

Author:
Maryam Mazaheri-Tirani; Soleyman Dayani
Source:
Plant cell, tissue, and organ culture 2020 v.140 no.2 pp. 279-289
ISSN:
0167-6857
Subject:
2,2-diphenyl-1-picrylhydrazyl; 2,4-D; Nicotiana tabacum; X-ray diffraction; antioxidant activity; callus; cytotoxicity; dose response; energy-dispersive X-ray analysis; enzyme activity; explants; nanoparticles; naphthaleneacetic acid; oxidative stress; petioles; protein content; superoxide dismutase; tobacco; zinc; zinc oxide; zinc sulfate
Abstract:
... Nano-technology has changed the properties of metal elements’ delivery into and effect on living systems. The current study, first, evaluated different combinations of plant growth regulators NAA, 2,4-D and KIN on Nicotiana tabacum callus induction from root, internode, petiole and leaf explants. Two mg L⁻¹ NAA with 0.1 mg L⁻¹ KIN on Murashige and Skoog (MS) medium induced calli in all explant sou ...
DOI:
10.1007/s11240-019-01725-0

2. Extracellular ATP promoted pollen germination and tube growth of Nicotiana tabacum through promoting K+ and Ca2+ absorption

Author:
Yansheng Wu; Baozhi Qin; Kaili Feng; Ruolin Yan; Erfang Kang; Ting Liu; Zhonglin Shang
Source:
Plant reproduction 2018 v.31 no.4 pp. 399-410
ISSN:
2194-7953
Subject:
Nicotiana tabacum; absorption; adenine; adenosine; adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; calcium; calcium channels; chelating agents; dose response; ethylene glycol tetraacetic acid; phosphates; plant growth; pollen; pollen tubes; potassium; potassium channels; protoplasts; stress tolerance
Abstract:
... Extracellular ATP (eATP) plays an essential role in plant growth, development, and stress tolerance. Here, we report that eATP participated in Nicotiana tabacum pollen germination (PG) and pollen tube growth (PTG) by regulating K⁺ and Ca²⁺ influx. Exogenous ATP or ADP effectively promoted PG and PTG in a dose-dependent manner; weakly hydrolysable ATP analog (ATPγS) showed a similar effect. AMP, ad ...
DOI:
10.1007/s00497-018-0341-6

3. Alleviation of cadmium toxicity by potassium supplementation involves various physiological and biochemical features in Nicotiana tabacum L.

Author:
Xinke Wang; Min Shi; Pengfei Hao; Weite Zheng; Fangbin Cao
Source:
Acta physiologiae plantarum 2017 v.39 no.6 pp. 132
ISSN:
0137-5881
Subject:
Nicotiana tabacum; cadmium; carbon dioxide; copper; dose response; greenhouses; growth retardation; heavy metals; hydroponics; iron; malondialdehyde; manganese; oxidative stress; photosynthesis; phytomass; pollutants; potassium; potassium bicarbonate; roots; shoots; soil; stomatal conductance; superoxide dismutase; tobacco; toxicity; zinc
Abstract:
... Potassium (K⁺) plays important roles in the development of plants and the response to various environmental stresses. However, the involvement of potassium in alleviating heavy metal stress in tobacco remains elusive. Greenhouse hydroponic experiments were conducted to evaluate the alleviating effects of K⁺ on tobacco subjected to cadmium (Cd) toxicity using four different K⁺ levels. Dose-dependen ...
DOI:
10.1007/s11738-017-2424-7

4. Benzoylsalicylic acid derivatives as defense activators in tobacco and Arabidopsis

Author:
Samuel Kamatham; Reddanna Pallu; Anil Kumar Pasupulati; Surya Satyanarayana Singh; Padmaja Gudipalli
Source:
Phytochemistry 2017 v.143 pp. 160-169
ISSN:
0031-9422
Subject:
Arabidopsis; Nicotiana tabacum; Tobacco mosaic virus; chemical structure; chlorides; dose response; elicitors; gene expression; genes; genetic markers; genetically modified organisms; immunity; infrared spectroscopy; leaves; necrosis; nuclear magnetic resonance spectroscopy; pathogens; salicylic acid; spectrometers; systemic acquired resistance; tobacco
Abstract:
... Systemic acquired resistance (SAR) is a long lasting inducible whole plant immunity often induced by either pathogens or chemical elicitors. Salicylic acid (SA) is a known SAR signal against a broad spectrum of pathogens in plants. In a recent study, we have reported that benzoylsalicylic acid (BzSA) is a SAR inducer in tobacco and Arabidopsis plants. Here, we have synthesized BzSA derivatives usi ...
DOI:
10.1016/j.phytochem.2017.07.014

5. Decay of Quinclorac in Acidic Paddy Soil and Risk Evaluation to the Subsequent Crop, Tobacco (Nicotiana tabacum L.)

Author:
Qiuzan Zhong; Shuqing Wan; Changyou Shen; Yi Liu
Source:
Bulletin of environmental contamination and toxicology 2018 v.101 no.2 pp. 284-287
ISSN:
0007-4861
Subject:
Nicotiana tabacum; Oryza sativa; dose response; field experimentation; half life; harvest date; leaves; paddies; paddy soils; phytotoxicity; quinclorac; rice; risk; risk assessment; seedlings; tobacco; weed control; weeds; China
Abstract:
... Quinclorac is a selective herbicide commonly used in China to control monocotyledonous weeds in paddy fields. A field experiment was conducted to quantify the environmental behavior of quinclorac in acidic paddy soil under rice (Oryza sativa L.) field conditions, and to evaluate the risk of its residues to the subsequent crop of tobacco (Nicotiana tabacum L.). Rice was sprayed once with quinclorac ...
DOI:
10.1007/s00128-018-2372-y

6. Inhibitory effect of gamma irradiation against Cucumber green mottle mosaic virus

Author:
Hong Jin-Sung; Jeong Mi-Ae; Jeong Rae-Dong
Source:
Plant Protection Science v.53 no.4 pp. 201-207
ISSN:
1212-2580
Subject:
Cucumber green mottle mosaic virus; Nicotiana tabacum; RNA; dose response; fungi; gamma radiation; genomics; pathogenicity; plant protection; transmission electron microscopy; virion; viruses
Abstract:
... Gamma irradiation has been shown to be an effective method of controlling plant bacterial and fungal pathogens, but data on its effect against plant viruses is limited. A mechanism for the inactivation of plant viruses by gamma irradiation has not been proposed. Gamma irradiation was evaluated for the inactivation of Cucumber green mottle mosaic virus (CGMMV) in Nicotiana tabacum plants. CGMMV inf ...
DOI:
10.17221/173/2016-pps

7. Toxicity impacts of chemically and biologically synthesized CuO nanoparticles on cell suspension cultures of Nicotiana tabacum

Author:
Sepideh Mahjouri; Ali Movafeghi; Baharak Divband; Morteza Kosari-Nasab
Source:
Plant cell, tissue, and organ culture 2018 v.135 no.2 pp. 223-234
ISSN:
0167-6857
Subject:
Nicotiana tabacum; cell biology; cell suspension culture; copper nanoparticles; cupric oxide; dose response; enzyme activity; malondialdehyde; plant extracts; polymers; pyrolysis; quality of life; risk; tobacco; toxicity; viability
Abstract:
... Nanotechnology has quite a lot of applications in various fields of industrial sectors like food and agriculture. Although nanotechnology can improve the quality of life, its possible associated risks should be assessed. Here copper oxide nanoparticles (CuO NPs) were synthesized by chemical (polymer pyrolysis) and biological (green) methods with an average size of 30 and 44 nm, respectively. After ...
DOI:
10.1007/s11240-018-1458-x

8. Prevention of Copper-Induced Calcium Influx and Cell Death by Prion-Derived Peptide in Suspension-Cultured Tobacco Cells

Author:
Tomoko Kagenishi; Ken Yokawa; Masaki Kuse; Minoru Isobe; François Bouteau; Tomonori Kawano
Source:
Zeitschrift für Naturforschung 2014 v.64 no.5-6 pp. 411-417
ISSN:
1865-7125
Subject:
Nicotiana tabacum; aequorin; calcium; calcium channels; calcium signaling; cell death; cell suspension culture; chemical reactions; copper; dose response; genes; humans; hydroxyl radicals; luminescence; neurotoxicity; phytoremediation; prions; roots; synthetic peptides; tobacco; transgenic plants
Abstract:
... Impact of copper on the oxidative and calcium signal transductions leading to cell death in plant cells and the effects of the copper-binding peptide derived from the human prion protein (PrP) as a novel plant-protecting agent were assessed using a cell suspension culture of transgenic tobacco (Nicotiana tabacum L., cell line BY-2) expressing the aequorin gene. Copper induces a series of biologica ...
DOI:
10.1515/znc-2009-5-619

9. Experimental Measurements and Mathematical Modeling of Cytosolic Ca2+ Signatures upon Elicitation by Penta-N-acetylchitopentaose Oligosaccharides in Nicotiana tabacum Cell Cultures

Author:
Kalina Mrozek; Karsten Niehaus; Petra Lutter
Source:
Plants 2013 v.2 no.4 pp. 750-768
ISSN:
2223-7747
Subject:
Nicotiana tabacum; calcium; calcium signaling; cell culture; cytoplasm; defense mechanisms; dose response; host-pathogen relationships; ions; oligosaccharides; pathogen-associated molecular patterns; pathogens; reactive oxygen species; simulation models
Abstract:
... Plants have developed sophisticated recognition systems for different kinds of pathogens. Pathogen-associated molecular patterns (PAMPs) can induce various defense mechanisms, e.g., the production of reactive oxygen species (ROS) as an early event. Plant defense reactions are initiated by a signal transduction cascade involving the release of calcium ions (Ca2+) from both external and internal sto ...
DOI:
10.3390/plants2040750
PubMed:
27137402
PubMed Central:
PMC4844394

10. Plant vaccination: Stimulation of defense system by caffeine production in planta

Author:
Yun-Soo Kim; Yong-Eui Choi; Hiroshi Sano
Source:
Plant signaling & behavior 2010 v.5 no.5 pp. 489-493
ISSN:
1559-2324
Subject:
Nicotiana tabacum; tobacco; Chrysanthemum; plant viruses; plant pathogenic bacteria; disease resistance; abiotic stress; stress tolerance; disease prevention; vaccination; caffeine; dose response; plant pests; herbivores; signal transduction; resistance mechanisms; secondary metabolites; biosynthesis; salicylic acid; plant breeding; genetic engineering; genetic transformation; transgenic plants; methyltransferases; gene transfer; antigen-antibody reactions
Abstract:
... Plants produce up to 100,000 secondary metabolites. One of their biological functions is self-denfese, and it is referred as chemical defense, directly and/or indirectly counteracting biotic and abiotic stresses. Alkaloids constitute 12% of total secondary metabolites, and some of them exhibit detrimental effects on living organisms. Caffeine (1,3,7-trimethylxanthine) is a member of purine alkaloi ...
DOI:
10.4161/psb.11087
PubMed:
20139738
PubMed Central:
PMC7080465

11. Reactive oxygen species formation and cell death in catalase-deficient tobacco leaf disks exposed to cadmium

Author:
Iannone, María Florencia; Rosales, Eliana Paola; Groppa, María Daniela; Benavides, María Patricia
Source:
Protoplasma 2010 v.245 no.1-4 pp. 15-27
ISSN:
0033-183X
Subject:
NAD(P)H oxidase (H2O2-forming); cultivars; genetically modified organisms; cell death; dose response; electrolytes; catalase; oxidative stress; hydrogen peroxide; lipid peroxidation; superoxide dismutase; NADP (coenzyme); cadmium; Nicotiana tabacum; tobacco; leaves
Abstract:
... The physiological responses of tobacco (Nicotiana tabacum L.) to oxidative stress induced by cadmium were examined with respect to reactive oxygen species (ROS) formation, antioxidant enzymes activities, and cell death appearance in wild-type SR1 and catalase-deficient CAT1AS plants. Leaf disks treated with 100 or 500 µM CdCl₂ increased Evans blue staining and leakage of electrolytes in SR1 or CAT ...
DOI:
10.1007/s00709-009-0097-9
PubMed:
20052507

12. Auxin concentration control of the average DNA content in cells of in vitro cultures: a theoretical model and comparison to experimental data for Allium cepa and Allium sativum

Author:
Rębilas, Krzysztof; Rębilas, Agata
Source:
Plant cell, tissue, and organ culture 2008 v.95 no.1 pp. 89-99
ISSN:
0167-6857
Subject:
simulation models; cell cycle; dose response; Allium cepa; garlic; Allium sativum; dosage; model validation; cultured cells; onions; Nicotiana tabacum; 2,4-D; culture media; callus; tissue culture
Abstract:
... A theoretical model explaining the average DNA content in cells of in vitro cultures as a function of concentration of auxin 2,4-D in medium is provided. The model assumes influence of auxin on the functioning of the main G1/S and G2/M control points of the cell cycle. Theoretical results are compared to experimental data obtained from the callus cultures of Allium sativum and Allium cepa conducte ...
DOI:
10.1007/s11240-008-9419-4

13. Plant growth depression by selective irradiation of the meristem with CO2 and diode lasers

Author:
Wöltjen, C.; Haferkamp, H.; Rath, T.; Herzog, D.
Source:
Biosystems engineering 2008 v.101 no.3 pp. 316-324
ISSN:
1537-5110
Subject:
weed control; carbon dioxide; meristems; lasers; Nicotiana tabacum; Echinochloa crus-galli; dose response; plant growth control; absorption; energy balance
Abstract:
... The application of CO2 and diode lasers as an alternative thermal method for the non-chemical control of weeds is investigated. Under laboratory conditions, experiments were carried out on the interaction of laser light with mono- and dicotyledonous plants represented by Echinochloa crus-galli and Nicotiana tabacum. Two laser systems, three growth stages and different energy doses were used. The f ...
DOI:
10.1016/j.biosystemseng.2008.08.006

14. Tobacco mosaic virus 126-kDa Protein Increases the Susceptibility of Nicotiana tabacum to Other Viruses and Its Dosage Affects Virus-Induced Gene Silencing

Author:
Harries, Phillip A.; Palanichelvam, Karuppaiah; Bhat, Sumana; Nelson, Richard S.
Source:
Molecular plant-microbe interactions 2008 v.21 no.12 pp. 1539-1548
ISSN:
0894-0282
Subject:
Tobacco mosaic virus; plant diseases and disorders; RNA interference; dose response; phenotype; dosage; gene expression; viral proteins; Nicotiana tabacum; disease resistance; molecular weight; tobacco
Abstract:
... The Tobacco mosaic virus (TMV) 126-kDa protein is a suppressor of RNA silencing previously shown to delay the silencing of transgenes in Nicotiana tabacum and N. benthamiana. Here, we demonstrate that expression of a 126-kDa protein-green fluorescent protein (GFP) fusion (126-GFP) in N. tabacum increases susceptibility to a broad assortment of viruses, including Alfalfa mosaic virus, Brome mosaic ...
DOI:
10.1094/MPMI-21-12-1539
PubMed:
18986250

15. Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity

Author:
Gorinova, N.; Nedkovska, M.; Todorovska, E.; Simova-Stoilova, L.; Stoyanova, Z.; Georgieva, K.; Demirevska-Kepova, K.; Atanassov, A.; Herzig, R.
Source:
Environmental pollution 2007 v.145 no.1 pp. 161-170
ISSN:
0269-7491
Subject:
ascorbic acids; plant response; toxicity testing; roots; oxidative stress; polypeptides; glutathione; dose response; soil pollution; cadmium; gene expression; phytoremediation; Nicotiana tabacum; photosynthesis; peroxidase; leaves; plant growth; bioaccumulation; enzyme activity; thiols; tobacco; genetic transformation; phytotoxicity; transgenic plants; metallothionein; Silene vulgaris
DOI:
10.1016/j.envpol.2006.03.025
PubMed:
16762468

16. Meropenem and moxalactam: Novel β-lactam antibiotics for efficient Agrobacterium-mediated transformation

Author:
Ogawa, Y.; Mii, M.
Source:
Plant science 2007 v.172 no.3 pp. 564-572
ISSN:
0168-9452
Subject:
tomatoes; genetic transformation; Southern blotting; cefotaxime; dose response; rice; moxalactam; Oryza sativa; Agrobacterium radiobacter; transgenic plants; polymerase chain reaction; Nicotiana tabacum; tobacco; genetic vectors; carbenicillin; Solanum lycopersicum var. lycopersicum
DOI:
10.1016/j.plantsci.2006.11.003

17. Integrated Application of Physiological and Molecular Methods to Forecast Determinative Morphogenetic Events in Tissue Cultured Tobacco (Nicotiana tabacum L. cv Samsun) Leaf Discs

Author:
Toldi, O.; Ahanen, K.; Kovacs, G.; Sorvari, S.; Toth, S.; Dulai, S.; Scott, P.
Source:
Plant growth regulation 2005 v.47 no.1 pp. 59-64
ISSN:
0167-6903
Subject:
cell differentiation; dose response; DNA methylation; morphogenesis; plant growth substances; explants; ontogeny; net assimilation rate; cell respiration; metabolites; photosynthesis; Nicotiana tabacum; genetic markers; tobacco; in vitro regeneration; culture media; leaves; tissue culture
Abstract:
... The purpose of this study was to search for physiological parameters that provide an early indication of the morphogenetic response of leaf disc explants to different tissue culture-level manipulations in order to design an accelerated optimisation process for this technology. Tobacco (Nicotiana tabacum L. cv. Samsun) was chosen as model in our studies, because this is still one of the most widely ...
DOI:
10.1007/s10725-005-1714-z

18. Exogenous calcium enhances the formation of vegetative buds, flowers and roots in tobacco pith explants cultured in the absence of exogenous hormones

Author:
Capitani, F.; Altamura, M.M.
Source:
Plant cell, tissue, and organ culture 2004 v.77 no.1 pp. 1-10
ISSN:
0167-6857
Subject:
developmental stages; micropropagation; roots; callus; dose response; explants; Nicotiana tabacum; in vitro regeneration; apical meristems; buds; calcium; culture media; tobacco; rhizogenesis; internodes; flowers; pith; caulogenesis; plant morphology
Abstract:
... Pith explants excised from the apical stem internodes of vegetative, flowering, and fruiting tobacco plants were cultured on hormone-free medium in the presence or absence of CaCl2 (3 mM). The aim was to determine the role of exogenous calcium (Ca2+), applied at the concentration normally present in the Murashige and Skoog (1962) medium, in organ formation obtainable in the absence of the exogenou ...

19. Chemical inactivation of the cinnamate 4-hydroxylase allows for the accumulation of salicylic acid in elicited cells

Author:
Schoch, G.A.; Nikov, G.N.; Alworth, W.L.; Werck-Reichhart, D.
Source:
Plant physiology 2002 v.130 no.2 pp. 1022-1031
ISSN:
0032-0889
Subject:
Nicotiana tabacum; tobacco; cell lines; cell suspension culture; plant proteins; oxygenases; cytochrome P-450; enzyme activity; enzyme inhibitors; salicylic acid; biosynthesis; chemical structure; phenylpropanoids; biochemical pathways; dose response; phenylalanine ammonia-lyase; chemical constituents of plants
Abstract:
... The cinnamate (CA) 4-hydroxylase (C4H) is a cytochrome P450 that catalyzes the second step of the main phenylpropanoid pathway, leading to the synthesis of lignin, pigments, and many defense molecules. Salicylic acid (SA) is an essential trigger of plant disease resistance. Some plant species can synthesize SA from CA by a mechanism not yet understood. A set of specific inhibitors of the C4H, incl ...
PubMed:
12376665
PubMed Central:
PMC166627

20. Cleavage of poly(ADP-ribose) polymerase during apoptosis induced by nicotinamide at high concentrations in tobacco suspension cells

Author:
Zhang, G.Y.; Zhu, R.Y.; Tian, R.H.; Dai, Y.R.
Source:
Plant growth regulation 2003 v.41 no.1 pp. 93-98
ISSN:
0167-6903
Subject:
nicotinamide; dose response; caspases; cultured cells; Nicotiana tabacum; tobacco; NAD ADP-ribosyltransferase; cell suspension culture; apoptosis; plant proteins
Abstract:
... Apoptosis induced by high concentrations of nicotinamide in tobacco suspension cells was observed. When cells were treated with 250 mM nicotinamide for 24 h, the hallmarks of apoptosis were detected, including DNA fragments increasing in size by multiples of 180-200 bp, condensation and peripheral distribution of nuclei chromatin and positive reaction to the TUNEL assay. In addition, the degradati ...

21. Supplemental boron stimulates ammonium assimilation in leaves of tobacco plants (Nicotiana tabacum L.)

Author:
Lopez-Lefebre, L.R.; Ruiz, J.M.; Rivero, R.M.; Garcia, P.C.; Sanchez, E.; Romero, L.
Source:
Plant growth regulation 2002 v.36 no.3 pp. 231-236
ISSN:
0167-6903
Subject:
Nicotiana tabacum; tobacco; leaves; ammonia; boron; dose response; amino acids; plant proteins; sugars; quantitative analysis; enzyme activity; glutamate-ammonia ligase; phosphoenolpyruvate carboxylase; chemical constituents of plants; glutamate dehydrogenase; biomass production
Abstract:
... The aim of this work was to analyse the response of NH4+ assimilation in leaves of tobacco plants (Nicotiana tabacum L. cv. Tennessee 86), to different B applications (B1, 5 micromolar H3BO3; B2, 10 micromolar H3BO3; B3, 20 micromolar H3BO3). The plants were grown under controlled environmental conditions and received a complete nutrient solution. In this experiment, we analysed the foliar concent ...

22. Auxin-gibberellin interactions and their role in plant growth

Author:
Ross, J.J.; O'Neill, D.P.; Wolbang, C.M.; Symons, G.M.; Reid, J.B.
Source:
Journal of plant growth regulation 2001 v.20 no.4 pp. 346-353
ISSN:
0721-7595
Subject:
biosynthesis; dose response; internodes; biochemical pathways; Nicotiana tabacum; length; gibberellins; oxidation; Pisum sativum; measurement; indole acetic acid
Abstract:
... Recently it was discovered that auxin promotes gibberellin (GA) biosynthesis in decapitated stems of pea (Pisum sativum L.) and tobacco (Nicotiana tabacum L.), and here we review the evidence for this interaction. We also discuss the possible relationship between auxin and the mechanisms by which bioactive GAs (such as GA1) regulate their own levels, and the implications of the auxin-GA interactio ...
DOI:
10.1007/s003440010034

23. Cyanobacterial GR6 glutamate-1-semialdehyde aminotransferase: a novel enzyme-based selectable marker for plant transformation

Author:
K.C. Gough; W.S. Hawes; J. Kilpatrick; G.C. Whitelam
Source:
Plant cell reports 2001 v.20 no.4 pp. 296-300
ISSN:
0721-7714
Subject:
genetic transformation; gene transfer; in vitro culture; dose response; antibiotics; transaminases; transgenic plants; Agrobacterium radiobacter; gene expression; Nicotiana tabacum; genes; micropropagation; Cyanobacteria; culture media; genetic markers
Abstract:
... The mutant glutamate-1-semialdehyde aminotransferase (GSA-AT) enzyme encoded by the hemL gene of the gabaculine-resistant cyanobacterium Synechococcus PCC6301 strain GR6 was expressed in tobacco following Agrobacterium-mediated transformation of leaf discs. When targeted to plastids, the GR6 hemL gene product conveyed gabaculine resistance to transgenic plants. Selection using 50 and 100 µM gabacu ...
DOI:
10.1007/s002990100337

24. Enhanced rate of multiplication and rooting through the use of coir in aseptic liquid culture media

Author:
Gangopadhyay, G.; Das, S.; Mitra, S.K.; Poddar, R.; Modak, B.K.; Mukherjee, K.K.
Source:
Plant cell, tissue, and organ culture 2002 v.68 no.3 pp. 301-310
ISSN:
0167-6857
Subject:
cell growth; developmental stages; micropropagation; in vitro culture; Andrographis paniculata; dose response; Chenopodium murale; Nicotiana tabacum; agar; leaves; coir; Chenopodium album; rooting; ultrastructure; culture media; Beta vulgaris; Musa; root hairs; Tectona grandis; plant morphology
Abstract:
... In the quest for cheap and suitable eco-friendly matrix with liquid culture to facilitate rooting three matrices, viz. paddy straw, jute and coir were chosen. Of the three matrices, coir was selected due to its higher water retention capacity compared to the other two matrices. Coir was used with aseptic liquid media for rooting of ten plant species, viz., Nicotiana tabacum, Andrographis paniculat ...

25. Farnesol-induced cell death and stimulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in tobacco cv Bright Yellow-2 cells

Author:
Hemmerlin, A.; Bach, T.J.
Source:
Plant physiology 2000 v.123 no.4 pp. 1257-1268
ISSN:
0032-0889
Subject:
dose response; cell growth; farnesol; cell division; enzyme inhibitors; cultured cells; Nicotiana tabacum; biomass production; enzyme activity; hydroxymethylglutaryl-CoA reductases; apoptosis; lovastatin
Abstract:
... Growth inhibition of tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells by mevinolin, a specific inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) could be partially overcome by the addition of farnesol. However, farnesol alone inhibited cell division and growth as measured by determination of fresh weight increase. When 7-d-old tobacco cv Bright Yellow-2 cells were diluted ...
PubMed:
10938345
PubMed Central:
PMC59085

26. Gene transfer into isolated and cultured tobacco zygotes by a specially designed device for electroporation

Author:
Li, S.T.; Yang, H.Y.
Source:
Plant cell reports 2000 v.19 no.12 pp. 1184-1187
ISSN:
0721-7714
Subject:
Nicotiana tabacum; genetic transformation; reporter genes; gene transfer; electroporation; transgenic plants; gene expression; in vitro culture; embryo (plant); culture media; benzyladenine; dose response; zygote; naphthaleneacetic acid
Abstract:
... We have established a technique for isolating, culturing and transforming tobacco zygotes. Zygotes were isolated by microdissection or enzymatic maceration from fertilized embryo sacs. Viable zygotes cocultured with mesophyll protoplasts underwent first division after 3 days of culture. Zygotes isolated by microdissection underwent a higher frequency of first division (61.2%) than those isolated b ...
DOI:
10.1007/s002990000249

27. Riboflavin induces disease resistance in plants by activating a novel signal transduction pathway

Author:
Dong, H.; Beer, S.V.
Source:
Phytopathology 2000 v.90 no.8 pp. 801-811
ISSN:
0031-949X
Subject:
Arabidopsis thaliana; Nicotiana tabacum; Peronospora parasitica; Alternaria alternata; plant pathogenic fungi; Pseudomonas syringae pv. tomato; plant pathogenic bacteria; Tobacco mosaic virus; pathogenicity; disease resistance; riboflavin; dose response; genes; protein synthesis; enzyme inhibitors; pathogenesis-related proteins
DOI:
10.1094/PHYTO.2000.90.8.801
PubMed:
18944500

28. Streptothricin resistance as a novel selectable marker for transgenic plant cells

Author:
Jelenska, J.; Tietze, E.; Tempe, J.; Brevet, J.
Source:
Plant cell reports 2000 v.19 no.3 pp. 298-303
ISSN:
0721-7714
Subject:
Arabidopsis thaliana; genetic transformation; gene transfer; in vitro culture; dose response; antibiotics; transgenic plants; Agrobacterium radiobacter; Rhizobium rhizogenes; Nicotiana tabacum; genes; benzyladenine; naphthaleneacetic acid; Lotus corniculatus var. japonicus; culture media; enzyme activity; genetic markers; acyltransferases
Abstract:
... Streptothricins are known as antimicrobial agents produced by Streptomyces spp. Bacterial resistance to streptothricin is mediated by specific enzymes exhibiting an acetyltransferase activity which renders the drug non-toxic for bacteria. The nucleotide sequence of several streptothricin resistance genes from bacteria have been described. Certain cells of eukaryotic parasites (such as Ustilago may ...
DOI:
10.1007/s002990050016

29. Evidence that auxin-induced growth of tobacco leaf tissues does not involve cell wall acidification

Author:
Keller, C.P.; Van Volkenburgh, E.
Source:
Plant physiology 1998 v.118 no.2 pp. 557-564
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; epinasty; dose response; cell walls; acidification; proton pump; osmotic pressure; measurement; membrane potential; pH; naphthaleneacetic acid; plasma membrane; ion exchange; fusicoccin
Abstract:
... Interveinal strips (10 x 1.5 mm) excised from growing tobacco (Nicotiana tabacum L. cv Xanthi) leaves have an auxin-specific, epinastic growth response that is developmentally regulated and is not the result of ethylene induction (C.P. Keller, E. Van Volkenburgh [1997] Plant Physiol 113:603-610). We report here that auxin (10 micromolar naphthalene acetic acid) treatment of strips does not result ...

30. Extending the microtubule/microfibril paradigm: Cellulose synthesis is required for normal cortical microtubule alignment in elongating cells

Author:
Fisher, D.D.; Cyr, R.J.
Source:
Plant physiology 1998 v.116 no.3 pp. 1043-1051
ISSN:
0032-0889
Subject:
cortex; protoplasts; cell wall components; biosynthesis; dose response; cell growth; tubulin; polymerization; developmental stages; cellulose; isoxaben; Nicotiana tabacum; microtubules; plasma membrane; cell suspension culture
Abstract:
... The cortical microtubule array provides spatial information to the cellulose-synthesizing machinery within the plasma membrane of elongating cells. Until now data indicated that information is transferred from organized cortical microtubules to the cellulose-synthesizing complex, which results in the deposition of ordered cellulosic walls. How cortical microtubules become aligned is unclear. The l ...

31. Glycosylated triterpenoid saponin: a specific inhibitor of diguanylate cyclase from Acetobacter xylinum. Biological activity and distribution

Author:
Ohana, P.; Delmer, D.P.; Volman, G.; Benziman, M.
Source:
Plant & cell physiology 1998 v.39 no.2 pp. 153-159
ISSN:
0032-0781
Subject:
triterpenoid saponins; dose response; biosynthesis; chemical reactions; Acetobacter; chemical constituents of plants; guanylate cyclase; cellulose; enzyme inhibitors; Nicotiana tabacum; spectral analysis; cell suspension culture; kinetics; Pisum sativum; enzyme activity; etiolation; chemical structure; shoots
Abstract:
... In a recent paper (Ohana et al. 1998), we described the purification and structural characterization of a novel glycosidic triterpenoid saponin (GTS), a specific inhibitor of diguanylate cyclase (dgc), the key regulatory enzyme of the cellulose synthesizing apparatus of the bacterium Acetobacter xylinum. This compound and an identical or very similar one were isolated from pea (Pisum sativum), and ...
DOI:
10.1093/oxfordjournals.pcp.a029352

32. Opposite effects on Spodoptera littoralis larvae of high expression level on a trypsin proteinase inhibitor in transgenic plants

Author:
De Leo, F.; Bonade-Bottino, M.A.; Ceci, L.R.; Gallerani, R.; Jouanin, L.
Source:
Plant physiology 1998 v.118 no.3 pp. 997-1004
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; insect pests; dose response; Spodoptera littoralis; trypsin inhibitors; transgenic plants; gene expression; Nicotiana tabacum; larvae; pest resistance; leaves
Abstract:
... This work illustrates potential adverse effects linked with the expression of proteinase inhibitor (PI) in plants used as a strategy to enhance pest resistance. Tobacco (Nicotiana tabacum L. cv Xanthi) and Arabidopsis [Heynh.] ecotype Wassilewskija) transgenic plants expressing the mustard trypsin PI 2 (MTI-2) at different level were obtained. First-instar larvae of the Egyptian cotton worm (Spodo ...

33. Regulation of leaf senescence by cytokinin, sugars, and light. Effects on NADH-dependent hydroxypyruvate reductase

Author:
Wingler, A.; Schaewen, A. von.; Leegood, R.C.; Lea, P.J.; Quick, W.P.
Source:
Plant physiology 1998 v.116 no.1 pp. 329-335
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; protein synthesis; dose response; NAD (coenzyme); pyruvic acid; chemical constituents of plants; glucose; transgenic plants; light intensity; oxidoreductases; cytokinins; Nicotiana tabacum; fructose; photosynthesis; enzyme activity; leaves
Abstract:
... The aim of this study was to investigate the interactions between cytokinin, sugar repression, and light in the senescence-related decline in photosynthetic enzymes of leaves. In transgenic tobacco (Nicotiana tabacum) plants that induce the production of cytokinin in senescing tissue, the age-dependent decline in NADH-dependent hydroxypyruvate reductase (HPR), ribulose-1,5-bisphosphate carboxylase ...

34. Spermine is a salicylate-independent endogenous inducer for both tobacco acidic pathogenesis-related proteins and resistance against tobacco mosaic virus infection

Author:
Yamakawa, H.; Kamada, H.; Satoh, M.; Ohashi, Y.
Source:
Plant physiology 1998 v.118 no.4 pp. 1213-1222
ISSN:
0032-0889
Subject:
Nicotiana tabacum; Tobacco mosaic virus; leaves; pathogenicity; protein synthesis; spermine; dose response; disease resistance; salicylates; gene expression; biochemical pathways; pathogenesis-related proteins
Abstract:
... Intercellular spaces are often the first sites invaded by pathogens. In the spaces of tobacco mosaic virus (TMV)-infected and necrotic lesion-forming tobacco (Nicotiana tabacum L.) leaves, we found that an inducer for acidic pathogenesis-related (PR) proteins was accumulated. The induction activity was recovered in gel-filtrated fractions of low molecular mass with a basic nature, into which authe ...

35. Timentin as an alternative antibiotic for suppression of Agrobacterium tumefaciens in genetic transformation

Author:
Cheng, Z.M.; Schnurr, J.A.; Kapaun, J.A.
Source:
Plant cell reports 1998 v.17 no.8 pp. 646-649
ISSN:
0721-7714
Subject:
genetic transformation; cefotaxime; ticarcillin; dose response; in vitro culture; clavulanic acid; carbenicillin; Agrobacterium radiobacter; Ulmus pumila; Nicotiana tabacum; culture media; leaves; shoots
Abstract:
... The effects of timentin on shoot regeneration of tobacco (Nicotiana tabaccum) and Siberian elm (Ulmus pumila L.) and its use for the suppression of Agrobacterium tumefaciens in Agrobacterium-mediated genetic transformation were determined. Timentin is a mixture of ticarcillin and clavulanic acid, and at concentrations of 200-500 mg/l with ratios of ticarcillin:clavulanic acid of 50:1 and 100:1, it ...
DOI:
10.1007/s002990050458

36. Transcriptional down-regulation by abscisic acid of pathogenesis-related beta-1,3-glucanase genes in tobacco cell cultures

Author:
Rezzonico, E.; Flury, N.; Meins, F. Jr.; Beffa, R.
Source:
Plant physiology 1998 v.117 no.2 pp. 585-592
ISSN:
0032-0889
Subject:
Nicotiana tabacum; cell suspension culture; leaves; pathogenesis; beta-glucanase; isozymes; chitinase; enzyme activity; abscisic acid; dose response; auxins; cytokinins; culture media; messenger RNA; gene expression; reporter genes; explants; transcription (genetics)
Abstract:
... Class I isoforms of beta-1,3-glucanases (betaGLU I) and chitinases (CHN I) are antifungal, vacuolar proteins implicated in plant defense. Tobacco (Nicotiana tabacum L.) betaGLU I and CHN I usually exhibit tightly coordinated developmental, hormonal, and pathogenesis-related regulation. Both enzymes are induced in cultured cells and tissues of cultivar Havana 425 tobacco by ethylene and are down-re ...
PubMed:
9625711
PubMed Central:
PMC34978

37. Auxin-induced epinasty of tobacco leaf tissues. A nonethylene-mediated response

Author:
Keller, C.P.; Van Volkenburgh, E.
Source:
Plant physiology 1997 v.113 no.2 pp. 603-610
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell growth; epinasty; dose response; acetic acid; ethylene; biosynthesis; naphthaleneacetic acid; aminooxyacetic acid; indole acetic acid
Abstract:
... Interveinal strips (10 x 1.5 mm) excised from growing tobacco (Nicotiana tabacum L. cv Xanthi) leaves curled >300 degrees when incubated for 20 h in 5 to 500 micromolar alpha-naphthalene acetic acid or 50 to 500 micromolar indole-3-acetic acid. Epinasty was not induced without auxin or by the auxin analog beta-naphthalene acetic acid, and less substantial epinasty was induced in midrib and vein se ...

38. Heat-shock-induced changes in intracellular Ca2+ level in tobacco seedlings in relation to thermotolerance

Author:
Gong, M.; Luit, A.H. van der.; Knight, M.R.; Trewavas, A.J.
Source:
Plant physiology 1998 v.116 no.1 pp. 429-437
ISSN:
0032-0889
Subject:
ethylene; dose response; quantitative analysis; heat tolerance; seedlings; calcium; transgenic plants; stress response; heat stress; gene expression; Nicotiana tabacum; chemical constituents of plants; chloroplasts
Abstract:
... Exposure of plants to elevated temperatures results in a complex set of changes in gene expression that induce thermotolerance and improve cellular survival to subsequent stress. Pretreatmentof young tobacco (Nicotiana plumbaginifolia) seedlings with Ca2+ or ethylene glycol-bis(beta-aminoethylether)-N,N,N', N'-tetraacetic acid enhanced or diminished subsequent thermotolerance, respectively, compar ...

39. Induction of PR-1 proteins and potentiation of pathogen signals by salicylic acid exhibit the same dose response and structural specificty in plant cell cultures

Author:
Xie, Z.; Fan, B.; Chen, Z.
Source:
Molecular plant-microbe interactions 1998 v.11 no.6 pp. 568-571
ISSN:
0894-0282
Subject:
Nicotiana tabacum; cultured cells; salicylic acid; dose response; disease resistance; Glycine max; leaves; metabolism; benzoic acid; catalase; enzyme activity; pathogenesis-related proteins; systemic acquired resistance; hypersensitive response
Abstract:
... Based on the marked difference in both dose response and structural specificity, it has been recently proposed that the induction of acquired resistance and pathogenesis- related (PR) proteins in intact plant leaves and the potentiation of pathogen signals in plant cell cultures by salicylic acid (SA) and its analogues are mediated by different SA signaling pathways initiated from different SA rec ...
DOI:
10.1094/MPMI.1998.11.6.568

40. Involvement of thylakoid overenergization in tentoxin-induced chlorosis in Nicotiana spp

Author:
Holland, N.; Evron, Y.; Jansen, M.A.K.; Edelman, M.; Pick, U.
Source:
Plant physiology 1997 v.114 no.3 pp. 887-892
ISSN:
0032-0889
Subject:
seedlings; plant pathogenic fungi; Nicotiana plumbaginifolia; dose response; Nicotiana tabacum; Alternaria alternata; chlorosis; oxygen; adenosinetriphosphatase; superoxide dismutase; adenosine triphosphate; pathogenicity; enzyme activity; gramicidin; ascorbic acid; biosynthesis; free radicals; thylakoids; transgenic plants; photophosphorylation; mutants
Abstract:
... The purpose of this work was to clarify the mechanism of tentoxin-induced chlorosis inNicotiana spp. seedlings. We found that chlorosis does not correlate with the inhibition of chloroplast ATP synthesis in vivo, since it occurs at tentoxin concentrations far higher than that required for the inhibition of photophosphorylation measured in the same seedlings. However, tentoxin-induced chlorosis doe ...

41. Local and systemic responses to antioxidants to tobacco mosaic virus infection and to salicylic acid to tobacco. Role in systemic acquired resistance

Author:
Fodor, J.; Gullner, G.; Adam, A.L.; Barna, B.; Komives, T.; Kiraly, Z.
Source:
Plant physiology 1997 v.114 no.4 pp. 1443-1451
ISSN:
0032-0889
Subject:
dehydroascorbic acid; necrosis; salicylic acid; Tobacco mosaic virus; glutathione; dose response; glutathione transferase; Nicotiana tabacum; disease resistance; leaves; quantitative analysis; superoxide dismutase; pathogenicity; enzyme activity; isozymes; antioxidants; ascorbic acid; glutathione-disulfide reductase; catalase; chemical constituents of plants
Abstract:
... Changes in ascorbate and glutathione levels and in activitiesof ascorbate peroxidase, catalase, dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione S-transferase (GST), and superoxide dismutase (SOD) were investigated in tobacco mosaic virus (TMV)-inoculated lower leaves and in noninoculated upper leaves of Nicotiana tabacum L. cv Xanthi-nc. In separate experiments the effec ...

42. Photoautotrophic tobacco cells adapted to grow at high salinity

Author:
Chang, C.C.; Locy, R.D.; Smeda, R.; Sahi, S.V.; Singh, N.K.
Source:
Plant cell reports 1997 v.16 no.7 pp. 495-502
ISSN:
0721-7714
Subject:
salt tolerance; dose response; quantitative analysis; salinity; glucose; cell culture; sucrose; thylakoids; net assimilation rate; starch; cultured cells; Nicotiana tabacum; sodium chloride; fructose; photosynthesis; protein content; culture media
Abstract:
... Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed ...
DOI:
10.1007/s002990050267

43. Quantum yields and rate constants of photochemical and nonphotochemical excitation quenching. Experiment and model

Author:
Laisk, A.; Oja, V.; Rasulov, B.; Eichelmann, H.; Sumberg, A.
Source:
Plant physiology 1997 v.115 no.2 pp. 803-815
ISSN:
0032-0889
Subject:
Helianthus annuus; ion transport; light intensity; photoinhibition; measurement; carbon dioxide; dose response; Gossypium hirsutum; light harvesting complex; electron transfer; Sorghum bicolor; Nicotiana tabacum; leaves; Amaranthus cruentus; mathematical models; equations; net assimilation rate; temperature; prediction; cytochrome b
Abstract:
... Sunflower (Helianthus annuus L.), cotton (Gossypium hirsutum L.), tobacco (Nicotiana tabacum L.), sorghum (Sorghum bicolor Moench.), amaranth (Amaranthus cruentus L.), and cytochrome b6f complex-deficient transgenic tobacco leaves were used to test the response of plants exposed to different light intensities and CO2 concentrations before and after photoinhibition at 4000 micromoles photons m-2 s- ...

44. The effects of salicylic acid and tobacco mosaic virus infection on the alternative oxidase of tobacco

Author:
Lennon, A.M.; Neuenschwander, U.H.; Ribas-Carbo, M.; Giles, L.; Ryals, J.A.; Siedow, J.N.
Source:
Plant physiology 1997 v.115 no.2 pp. 783-791
ISSN:
0032-0889
Subject:
Nicotiana tabacum; leaves; cell suspension culture; mitochondria; Tobacco mosaic virus; pathogenicity; disease resistance; salicylic acid; dose response; enzyme activity; oxygenases; pathogenesis; messenger RNA; measurement; cytochromes; transgenic plants; gene expression; electron transfer; systemic acquired resistance
Abstract:
... Salicylic acid (SA) is a signal in systemic acquired resistance and an inducer of the alternative oxidase protein in tobacco (Nicotiana tabacum cv Xanthi nc) cell suspensions and during thermogenesis in aroid spadices. The effects of SA on the levels of alternative oxidase protein and the pathogenesis-related 1a mRNA (a marker for systemic acquired resistance), and on the partitioning of electrons ...

45. Amyloplast formation in cultured tobacco cells; effects of plant hormones on multiplication, size, and starch content

Author:
Sakai, A.; Yashiro, K.; Kawano, S.; Kuroiwa, T.
Source:
Plant cell reports 1996 v.15 no.8 pp. 601-605
ISSN:
0721-7714
Subject:
in vitro culture; dose response; amylose; fluorides; biosynthesis; cell growth; dimethyl sulfoxide; cell division; indoles; plastids; benzyladenine; Nicotiana tabacum; naphthaleneacetic acid; chemical constituents of plants; 2,4-D; culture media
Abstract:
... In BY-2 cultured tobacco cells (Nicotiana tabacum L), depletion of 2,4-dichlorophenoxyacetic acid (2,4-D) and addition of benzyladenine (BA) caused amyloplast formation, a decrease in cell multiplication, and an increase in cell size. These changes were primarily triggered by the depletion of 2,4-D, and facilitated by the addition of BA. An increase in the starch content of BY-2 cells was always a ...
DOI:
10.1007/BF00232461
PubMed:
24178526

46. Attenuation of the phenotype caused by the root-inducing, left-hand, transferred DNA and its rolA gene: correlations with changes in polyamine metabolism and DNA methylation

Author:
Martin-Tanguy, J.; Sun, L.Y.; Burtin, D.; Vernoy, R.; Rossin, N.; Tepfer, D.
Source:
Plant physiology 1996 v.111 no.1 pp. 259-267
ISSN:
0032-0889
Subject:
Nicotiana tabacum; roots; genetic transformation; Rhizobium rhizogenes; DNA; genes; gene expression; genetic code; methylation; putrescine; tyramine; dose response; phenotype
Abstract:
... We present four examples of attenuation of the transformed phenotype caused by the root-inducing, left-hand, transferred DNA from Agrobacterium rhizogenes in tobacco (Nicotiana tabacum). The first was associated with a genetic variable (homozygosity for the T-DNA), and the second was induced at the physiological level by putrescine and tyramine, suggesting that the transformed phenotype depends on ...

47. Auxin-sensitive elements from promoters of tobacco GST genes and a consensus as-1-like element differ only in relative strength

Author:
Zaal, B.J. van der; Droog, F.N.J.; Pieterse, F.J.; Hooykaas, P.J.J.
Source:
Plant physiology 1996 v.110 no.1 pp. 79-88
ISSN:
0032-0889
Subject:
genetic transformation; dose response; glutathione transferase; transgenic plants; binding proteins; gene expression; Nicotiana tabacum; salicylic acid; reporter genes; benzyladenine; naphthaleneacetic acid; enzyme activity; 2,4-D; promoter regions
Abstract:
... We have investigated the cis-acting potential of several as elements (20-bp as-1/ocs-like sequences) in both yeast and plant cells. These TGACG[N7]TGACG-resembling elements were surprisingly similar with respect to their ability to confer inducibility by auxins and related compounds to a heterologous TATA box in stably transformed plant cells. Both in plant cells and in yeast it was found that dif ...
PubMed:
8587996
PubMed Central:
PMC157696

48. Effect of cadimum treatment on the expression of chimeric genes in transgenic tobacco seedlings and calli

Author:
Stefanov, I.; Frank, J.; Gedamu, L.; Misra, S.
Source:
Plant cell reports 1997 v.16 no.5 pp. 291-294
ISSN:
0721-7714
Subject:
Nicotiana tabacum; cadmium; dose response; phytotoxicity; plant breeding; chromosome mapping; gene transfer; transgenic plants; chimerism; reporter genes; gene expression; seedlings; callus; beta-glucuronidase; enzyme activity; measurement; glucuronic acid; transcription (genetics); promoter regions
Abstract:
... Transgenic tobacco plants and calli bearing the bacterial uidA A gene under the transcriptional control of rbcS, mas and CaMV35S promoter(s) were exposed to different concentrations of cadmium. The transcriptional activity of the promoters was monitored using p-nitrophenyl beta-D-glucuronide as a substrate for the beta-glucuronidase (uidA) reporter enzyme. The rbcS promoter was repressed by high c ...
DOI:
10.1007/BF01088283

49. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

50. Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells

Author:
Yamamoto, Y.; Hachiya, A.; Matsumoto, H.
Source:
Plant & cell physiology 1997 v.38 no.12 pp. 1333-1339
ISSN:
0032-0781
Subject:
Nicotiana tabacum; cell suspension culture; aluminum; iron; phytotoxicity; dose response; oxidation; lipid peroxidation; viability; cell growth; antioxidants; plasma membrane
Abstract:
... Aluminum (Al) and ferrous iron [Fe(II)] are separately non-toxic to cultured tobacco cells in nutrient solution. However, Al and Fe(II) together cause the peroxidation of membrane lipids, the accumulation of Al and Fe, and the loss of viability [Ono et at. (1995) Plant Cell Physiol. 36: 115]. We investigated the cause-and-effect relationships of these various responses. In cells exposed to Fe(II) ...
DOI:
10.1093/oxfordjournals.pcp.a029126