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You searched for: Subject Nicotiana tabacum Remove constraint Subject: Nicotiana tabacum Subject term esters Remove constraint Subject term: esters
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1. Isolation and characterization of the sucrose esters of the cuticular waxes of green tobacco leaf

Author:
Severson, R.F.; Arrendale, R.F.; Chortyk, O.T.; Green, C.R.; Thome, F.A.; Stewart, J.L.; Johnson, A.W.
Source:
Journal of agricultural and food chemistry 1985 v.33 no.5 pp. 870-875
ISSN:
0021-8561
Subject:
Nicotiana tabacum; sucrose; esters; waxes
DOI:
10.1021/jf00065a026

2. Rapid and simple method for estimation of sugar esters

Author:
Lin, Y.; Wagner, G.J.
Source:
Journal of agricultural and food chemistry 1994 v.42 no.8 pp. 1709-1712
ISSN:
0021-8561
Subject:
Nicotiana tabacum; trichomes; quantitative analysis; sugars; esters; histochemistry
Abstract:
... A procedure is described for a simple and highly sensitive quantitative assay of various sucrose and glucose ester mixtures extracted from plant tissue surfaces and of certain synthetic sugar esters of commercial importance. The method involves staining sugar esters with Rhodamine B followed by spectrophotometric detection of the "complexed" stain. The only instrumentation required consists of a c ...
DOI:
10.1021/jf00044a024

3. Biorationals from Nicotiana protect cucumbers against Colletotrichum lagenarium (Pass.) Ell. & Halst disease development

Author:
Kennedy, B.S.; Nielsen, M.T.; Severson, R.F.
Source:
Journal of chemical ecology 1995 v.21 no.2 pp. 221-231
ISSN:
0098-0331
Subject:
Nicotiana glutinosa; Colletotrichum orbiculare; germination; trichomes; plant extracts; plant pathogenic fungi; Nicotiana tabacum; esters; mixtures; biological control
Abstract:
... Trichome exudate compounds isolated from leaves of Nicotiana tabacum, N. glutinosa (accessions 24 and 24a), and 21 other Nicotiana species were evaluated for biorational activity against C. lagenarium, the anthracnose pathogen of cucumber. Exudate compounds tested were the cembrane diterpenes alpha and beta-4,8,13-duvatriene-1,3-diols (DVT diols); the labdane diterpenes (13-E)-labda-13-ene-8alpha, ...
DOI:
10.1007/BF02036653
PubMed:
24234021

4. Cell-free synthesis of pectin. Identification and partial characterization of polygalacturonate 4-alpha-galacturonosyltransferase and its products from membrane preparations of tobacco cell-suspension cultures

Author:
Doong, R.L.; Liljebjelke, K.; Fralish, G.; Kumar, A.; Mohnen, D.
Source:
Plant physiology 1995 v.109 no.1 pp. 141-152
ISSN:
0032-0889
Subject:
hexosyltransferases; radiolabeling; polygalacturonase; cell membranes; analytical methods; synthesis; Nicotiana tabacum; esters; cell wall components; galacturonic acid; pectins; bioassays; temperature; enzyme activity; esterification; pH; kinetics; cell suspension culture
Abstract:
... Polygalacturonate 4-alpha-galacturonosyltransferase (EC 2.4.1.43) activity has been identified in microsomal membranes isolated from tobacco (Nicotiana tabacum L. cv Samsun) cell-suspension cultures. Incubation of UDP-[14C]galacturonic acid with tobacco membranes results in a time-dependent incorporation of [14C]galacturonic acid into a chloroform-methanol-precipitable and 65% ethanol-insoluble pr ...

5. Induction of nopaline synthase promoter activity by H2O2 has no direct correlation with salicylic acid

Author:
Dai, Z.; An, G.
Source:
Plant physiology 1995 v.109 no.4 pp. 1191-1197
ISSN:
0032-0889
Subject:
recombinant DNA; salicylic acid; chloramphenicol acetyltransferase; dose response; jasmonic acid; nopaline; hydrogen peroxide; gene expression; Nicotiana tabacum; esters; oxygen; defense mechanisms; enzyme activity; pathogenesis-related proteins; biosynthesis; catalase; transgenic plants; biochemical pathways; mutants; reporter genes; kinetics
Abstract:
... Transgenic tobacco (Nicotiana tabacum L.) plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were tested for their response to treatment with H2O2. The nos promoter-driven CAT activity increased significantly by addition of H2O2, reaching the maximum level at 15 mM. Kinetic analysis for CAT activity showed that indu ...
PubMed:
8539287
PubMed Central:
PMC157649

6. Regulation by cytokinins of endogenous levels of jasmonic and salicylic acids in mechanically wounded tobacco plants

Author:
Sano, H.; Seo, S.; Koizumi, N.; Niki, T.; Iwamura, H.; Ohashi, Y.
Source:
Plant & cell physiology 1996 v.37 no.6 pp. 762-769
ISSN:
0032-0781
Subject:
Nicotiana tabacum; transgenic plants; mechanical damage; biosynthesis; jasmonic acid; esters; salicylic acid; glucosides; quantitative analysis; benzyladenine; guanosine triphosphate; binding proteins; protein synthesis; pathogenesis-related proteins; stress response
Abstract:
... Plants respond differentially to wounding and pathogens using distinct signaling pathways, so that wound signals are transmitted to jasmonic acid (JA) which induces basic pathogenesis-related (PR) proteins, whereas pathogenic signals cause, in addition to JA, accumulation of salicylic acid (SA) which stimulates production of acidic PR proteins. Transgenic tobacco plants expressing a gene for a sma ...
DOI:
10.1093/oxfordjournals.pcp.a029011

7. A possible mechanism for the oxidation of sinapyl alcohol by peroxidase-dependent reactions in the apoplast: enhancement of the oxidation by hydroxycinnamic acids and components of the apoplast

Author:
Takahama, U.; Oniki, T.; Shimokawa, H.
Source:
Plant & cell physiology 1996 v.37 no.4 pp. 499-504
ISSN:
0032-0781
Subject:
intercellular spaces; biosynthesis; stems; free radicals; Vigna angularis; sinapic acid; isozymes; guaiacol; cinnamic acid; peroxidases; ferulic acid; Nicotiana tabacum; esters; oxidation; enzyme activity; lignin; syringic acid
Abstract:
... Apoplastic peroxidase isoenzymes from stems of Nicotiana tabacum rapidly oxidized sinapic acid and sinapyl alcohol, in addition to 4-coumaric acid, ferulic acid and coniferyl alcohol. By contrast, the peroxidase isoenzymes from stems of Vigna angularis oxidized sinapic acid and sinapyl alcohol quite slowly but rapidly oxidized compounds with a 4-hydroxyphenyl or a guaiacyl group. However, the oxid ...
DOI:
10.1093/oxfordjournals.pcp.a028972

8. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

9. Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose

Author:
Durner, J.; Wendehenne, D.; Klessig, D.F.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1998 v.95 no.17 pp. 10328-10333
ISSN:
0027-8424
Subject:
Tobacco mosaic virus; messenger RNA; pathogenesis-related proteins; nitric oxide synthase; phenylalanine ammonia-lyase; nitric oxide; guanylate cyclase; cyclic GMP; gene expression; Nicotiana tabacum; esters; salicylic acid; enzyme activity; cell suspension culture; ribose
Abstract:
... Reactive oxygen species are believed to perform multiple roles during plant defense and possibly as cellular signaling molecules. In animals, nitric oxide (NO) is an important redox-active signaling molecule. Here we show that infection of resistant, but not susceptible, tobacco with tobacco mosaic virus resulted in enhanced NO synthase (NOS) activity. Furthermore, administration of NO donors or r ...
DOI:
10.1073/pnas.95.17.10328
PubMed:
9707647
PubMed Central:
PMC21508

10. Tobacco O-methyltransferases involved in phenylpropanoid metabolism. The different caffeoyl-coenzyme A/5-hydroxyferuloyl-coenzyme A 3/5-O-methyltransferase and caffeic acid/5-O-hydroxyferulic acid 3/5-O-methyltransferase classes have distinct substrate specificities and expression patterns

Author:
Maury, S.; Geoffroy, P.; Legrand, M.
Source:
Plant physiology 1999 v.121 no.1 pp. 215-223
ISSN:
0032-0889
Subject:
Nicotiana tabacum; transferases; phenylpropanoids; biosynthesis; gene expression; methylation; Tobacco mosaic virus; leaves; isozymes; Escherichia coli; esters; biochemical pathways; lignin
Abstract:
... The biosynthesis of lignin monomers involves two methylation steps catalyzed by orthodiphenol-O-methyltransferases: caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferases (COMTs) and caffeoyl-coenzyme A (CoA)/5-hydroxyferuloyl-CoA 3/5-O-methyltransferases (CCoAOMTs). Two COMT classes (I and II) were already known to occur in tobacco (Nicotiana tabacum) and three distinct CCoAOMT classes have ...

11. Characterization of an acyltransferase capable of synthesizing benzylbenzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri

Author:
D'Auria, J.C.; Chen, F.; Pichersky, E.
Source:
Plant physiology 2002 v.130 no.1 pp. 466-476
ISSN:
0032-0889
Subject:
stigma; Clarkia; plant proteins; gene expression; Nicotiana tabacum; leaves; Escherichia coli; Arabidopsis thaliana; volatile compounds; complementary DNA; benzyl benzoate; amino acid sequences; enzyme activity; tobacco; immunoblotting; biosynthesis; expressed sequence tags; flowers; biochemical pathways; chemical constituents of plants; protein content; acyltransferases
Abstract:
... A cDNA encoding a protein with 456 amino acids whose sequence shows considerable similarity to plant acyltransferases was identified among 750 Clarkia breweri flower expressed sequence tags. The cDNA was expressed in Escherichia coli, and the protein produced was shown to encode the enzyme benzoyl-coenzyme A (CoA):benzyl alcohol benzoyl transferase (BEBT). BEBT catalyzes the formation of benzylben ...
PubMed:
12226525
PubMed Central:
PMC166578

12. Molecular characterization of plant ubiquitin-conjugating enzymes belonging to the UbcP4/E2-C/UBCx/UbcH10 gene family

Author:
Criqui, M.C.; Almeida Engler, J. de.; Camasses, A.; Capron, A.; Parmentier, Y.; Inze, D.; Genschik, P.
Source:
Plant physiology 2002 v.130 no.3 pp. 1230-1240
ISSN:
0032-0889
Subject:
radishes; plant proteins; Southern blotting; lyases; recombinant proteins; Schizosaccharomyces pombe; cell division; gene expression; Nicotiana tabacum; esters; cytoplasm; Arabidopsis thaliana; messenger RNA; cell culture; histology; amino acid sequences; cell nucleus; ubiquitin; thiols; tobacco; green fluorescent protein; transgenic plants; Raphanus sativus; nucleotide sequences
Abstract:
... The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One class of E2s (called E2-C) seems specifically involved in cyclin B1 degradation. ...
PubMed:
12427990
PubMed Central:
PMC166644

13. Arabidopsis acyl-CoA-binding protein ACBP2 interacts with an ethylene-responsive element-binding protein, AtEBP, via its ankyrin repeats

Author:
Li, H.Y.; Chye, M.L.
Source:
Plant molecular biology 2004 v.54 no.2 pp. 233-243
ISSN:
0167-4412
Subject:
Arabidopsis thaliana; protein binding; transcription factors; cytochemistry; infection; Agrobacterium radiobacter; fatty acid-binding proteins; binding sites; esters; Nicotiana tabacum; plasma membrane; leaves; plant proteins
Abstract:
... Cytosolic acyl-CoA-binding proteins (ACBP) bind long-chain acyl-CoAs and act as intracellular acyl-CoA transporters and maintain acyl-CoA pools. Arabidopsis thaliana ACBP2 shows conservation at the acyl-CoA-binding domain to cytosolic ACBPs but is distinct by the presence of an N-terminal transmembrane domain and C-terminal ankyrin repeats. The function of the acyl-CoA-binding domain in ACBP2 has ...
PubMed:
15159625

14. Chemical composition of the ether and ethyl acetate extracts of Serbian selected tobacco types: Yaka, Prilep and Otlja

Author:
Radulovic, N.; Stojanovic, G.; Palic, R.; Alagic, S.
Source:
Journal of essential oil research 2006 v.18 no.5 pp. 562-565
ISSN:
1041-2905
Subject:
Nicotiana tabacum; cultivars; tobacco; leaves; plant extracts; leaf oils; chemical composition; chemical analysis; quantitative analysis; ethers; esters; supercritical fluid extraction; carbon dioxide; nicotine; volatile organic compounds; aromatic hydrocarbons; alkanes; Serbia
Abstract:
... A study of the chemical composition of ether and ethyl acetate extracts of the Serbian selected tobacco types Yaka, Prilep and Otlja was performed using GC and GC/MS. The major constituents were: nicotine (24.2-58.9% of the total material detected in the ethyl acetate extracts and 46.1-49.5% of ether extracts), neophytadiene (4.0-17.9% of ethyl acetate extracts and 11.2-31.9% of ether extracts) an ...
DOI:
10.1080/10412905.2006.9699168

15. The Jasmonate-Induced Expression of the Nicotiana tabacum Leaf Lectin

Author:
Lannoo, Nausicaä; Vandenborre, Gianni; Miersch, Otto; Smagghe, Guy; Wasternack, Claus; Peumans, Willy J.; Van Damme, Els J.M.
Source:
Plant & cell physiology 2007 v.48 no.8 pp. 1207-1218
ISSN:
0032-0781
Subject:
protein synthesis; jasmonic acid; exposure duration; herbivores; Nicotiana tabacum; esters; tobacco; insects; leaves; plant proteins
Abstract:
... Previous experiments with tobacco (Nicotiana tabacum L. cv Samsun NN) plants revealed that jasmonic acid methyl ester (JAME) induces the expression of a cytoplasmic/nuclear lectin in leaf cells and provided the first evidence that jasmonates affect the expression of carbohydrate-binding proteins in plant cells. To corroborate the induced accumulation of relatively large amounts of a cytoplasmic/nu ...
DOI:
10.1093/pcp/pcm090
PubMed:
17623741

16. Methylation of Gibberellins by Arabidopsis GAMT1 and GAMT2

Author:
Varbanova, Marina; Yamaguchi, Shinjiro; Yang, Yue; McKelvey, Katherine; Hanada, Atsushi; Borochov, Roy; Yu, Fei; Jikumaru, Yusuke; Ross, Jeannine; Cortes, Diego; Ma, Choong Je; Noel, Joseph P.; Mander, Lew; Shulaev, Vladimir; Kamiya, Yuji; Rodermel, Steve; Weiss, David; Pichersky, Eran
Source:
plant cell 2007 v.19 no.1 pp. 32-45
ISSN:
1040-4651
Subject:
Arabidopsis thaliana; Nicotiana tabacum; Petunia hybrida; biosynthesis; enzymes; esters; fruits; germination; gibberellins; methylation; mutants; seed development; seeds; tobacco
Abstract:
... Arabidopsis thaliana GAMT1 and GAMT2 encode enzymes that catalyze formation of the methyl esters of gibberellins (GAs). Ectopic expression of GAMT1 or GAMT2 in Arabidopsis, tobacco (Nicotiana tabacum), and petunia (Petunia hybrida) resulted in plants with GA deficiency and typical GA deficiency phenotypes, such as dwarfism and reduced fertility. GAMT1 and GAMT2 are both expressed mainly in whole s ...
DOI:
10.1105/tpc.106.044602
PubMed:
17220201
PubMed Central:
PMC1820973

17. Free and Conjugated Phytosterols in Cured Tobacco Leaves: Influence of Genotype, Growing Region, and Stalk Position

Author:
Liu, W.H.; Yong, G.P.; Fang, L.; Wang, S.K.; Bai, H.J.; Jiang, J.H.; Liu, S.M.
Source:
Journal of agricultural and food chemistry 2008 v.56 no.1 pp. 185-189
ISSN:
0021-8561
Subject:
phytosterols; chemical composition; stems; curing (nonfood products); genotype; esters; burley tobacco; Nicotiana tabacum; spatial variation; flue-cured tobacco; gas chromatography; glycosides; leaves
Abstract:
... Although phytosterols in tobacco leaves have specific effects on tobacco quality, there is little research on the distribution of free and conjugated phytosterols in various tobacco leaves. In this paper, we explored the content and composition of phytosterols in representative cured tobacco leaves by gas chromatography of TMS-ether derivatives. We found that phytosterol contents in tobacco leaves ...
DOI:
10.1021/jf0722708

18. Nitric Oxide Interacts with Salicylate to Regulate Biphasic Ethylene Production during the Hypersensitive Response

Author:
Mur, Luis A.J.; Laarhoven, Lucas J.J.; Harren, Frans J.M.; Hall, Michael A.; Smith, Aileen R.
Source:
Plant physiology 2008 v.148 no.3 pp. 1537-1546
ISSN:
0032-0889
Subject:
biosynthesis; tobacco; lasers; nitric oxide; avirulent strains; oxidative stress; systemic acquired resistance; ethylene production; Pseudomonas syringae; Nicotiana tabacum; esters; genes; leaves; salicylic acid; sodium; hypersensitive response; aminoethoxyvinylglycine
Abstract:
... C₂H₄ is associated with plant defense, but its role during the hypersensitive response (HR) remains largely uncharacterized. C₂H₄ production in tobacco (Nicotiana tabacum) following inoculation with HR-eliciting Pseudomonas syringae pathovars measured by laser photoacoustic detection was biphasic. A first transient rise (C₂H₄-I) occurred 1 to 4 h following inoculation with HR-eliciting, disease-fo ...
DOI:
10.1104/pp.108.124404
PubMed:
18799663
PubMed Central:
PMC2577248

19. Evidence for Unidirectional Flow through Plasmodesmata

Author:
Christensen, Nynne Meyn; Faulkner, Christine; Oparka, Karl
Source:
Plant physiology 2009 v.150 no.1 pp. 96-104
ISSN:
0032-0889
Subject:
green fluorescent protein; plasmodesmata; sodium azide; mass flow; transgenic plants; trichomes; solutes; gene expression; viruses; Nicotiana tabacum; esters; tobacco; metabolic inhibitors; fluorescein; leaves
Abstract:
... The leaf trichome of tobacco (Nicotiana tabacum) represents a unique secretory structure in which the basal trichome cell is connected to the epidermis by numerous plasmodesmata (PD). Small fluorescent probes microinjected into the basal trichome cell moved apically into distal trichome cells but not into the subtending epidermal cell. In marked contrast, the same probes moved apically into tricho ...
DOI:
10.1104/pp.109.137083
PubMed:
19270059
PubMed Central:
PMC2675744

20. Detoxification of Jatropha curcas kernel cake by a novel Streptomyces fimicarius strain

Author:
Xing-Hong Wang; Lingcheng Ou; Liang-Liang Fu; Shui Zheng; Ji-Dong Lou; José Gomes-Laranjo; Jiao Li; Changhe Zhang
Source:
Journal of hazardous materials 2013 v.260 pp. 238-246
ISSN:
0304-3894
Subject:
Jatropha curcas; Nicotiana tabacum; Streptomyces fimicarius; biodegradation; biodiesel; carp; esters; feeds; fingerlings; high performance liquid chromatography; mathematical models; oilseed cakes; organic fertilizers; root growth; seed germination; solid state fermentation; tobacco; toxicity; toxins
Abstract:
... A huge amount of kernel cake, which contains a variety of toxins including phorbol esters (tumor promoters), is projected to be generated yearly in the near future by the Jatropha biodiesel industry. We showed that the kernel cake strongly inhibited plant seed germination and root growth and was highly toxic to carp fingerlings, even though phorbol esters were undetectable by HPLC. Therefore it mu ...
DOI:
10.1016/j.jhazmat.2013.05.012

21. Metathesis of tobacco fatty acid methyl esters: Generation of industrially important platform chemicals

Author:
Yelchuri Vyshnavi; Rachapudi B.N. Prasad; Mallampalli S.L. Karuna
Source:
Industrial crops and products 2013 v.50 pp. 701-706
ISSN:
0926-6690
Subject:
Nicotiana tabacum; esters; hydrocarbons; oleic acid; polymers; tobacco; vegetable oil
Abstract:
... Self-methathesis of vegetable oil based unsaturated fatty acids provide a renewable and convenient route for the preparation of a number of platform chemicals useful for the production of polymers, biolubricants and other industrial products. The present study is focused on the use of the unsaturated non-edible oil methyl esters of tobacco (Nicotiana tabacum 82.2%) self- metathesis. Metathesis was ...
DOI:
10.1016/j.indcrop.2013.08.020

22. Subcellular localization of rice acyl‐CoA‐binding proteins (ACBPs) indicates that OsACBP6::GFP is targeted to the peroxisomes

Author:
Wei Meng; An‐Shan Hsiao; Caiji Gao; Liwen Jiang; Mee‐Len Chye
Source:
new phytologist 2014 v.203 no.2 pp. 469-482
ISSN:
0028-646X
Subject:
ABC transporters; Arabidopsis thaliana; Nicotiana tabacum; Oryza sativa; beta oxidation; endoplasmic reticulum; esters; fatty acids; gene overexpression; genes; green fluorescent protein; indole butyric acid; jasmonic acid; peroxisomes; rice; tobacco
Abstract:
... Acyl‐CoA‐binding proteins (ACBPs) show conservation at the acyl‐CoA‐binding (ACB) domain which facilitates binding to acyl‐CoA esters. In Arabidopsis thaliana, six ACBPs participate in development and stress responses. Rice (Oryza sativa) also contains six genes encoding ACBPs. We investigated differences in subcellular localization between monocot rice and eudicot A. thaliana ACBPs. The subcellul ...
DOI:
10.1111/nph.12809
PubMed:
24738983

23. Das Vorkommen von Glucosaminestern der Hydroxy-zimtsäuren in Gewebekulturen von Nicotiana tabacum

Author:
L. Bergmann; W. Thies; K. Erdelsky
Source:
Zeitschrift für Naturforschung 2014 v.20 no.12 pp. 1297-1301
ISSN:
0932-0776
Subject:
Nicotiana tabacum; esters; ferulic acid; glucosamine; glucose; spectral analysis; tissue culture; tobacco
Abstract:
... Tissue cultures of Nicotiana tabacum var. “Samsun” contain an array of phenolic compounds. Beside the known glucose and quinyl esters of the hydroxycinnamic acids three hitherto unreported phenolic acid esters were isolated from tobacco tissue cultures. The analytical and spectral data presented in this paper lead to the conclusion that these compounds are glucosamine esters of p-coumaric, caffeic ...
DOI:
10.1515/znb-1965-1223

24. A method for simultaneous analysis of phytosterols and phytosterol esters in tobacco leaves using non aqueous reversed phase chromatography and atmospheric pressure chemical ionization mass spectrometry detector

Author:
Naoyuki Ishida
Source:
Journal of chromatography 2014 v.1340 pp. 99-108
ISSN:
0021-9673
Subject:
Nicotiana tabacum; acetone; acetonitrile; atmospheric pressure; cluster analysis; detection limit; esters; ionization; leaves; linoleic acid; linolenic acid; mass spectrometry; monitoring; oleic acid; palmitic acid; phytosterols; regression analysis; reversed-phase liquid chromatography; stearic acid; tobacco
Abstract:
... While numerous analytical methods for phytosterols have been reported, the similar polarity and large molecules of phytosterol esters have made the methods lengthy and complicated. For this reason, an analytical method that could completely separate phytosterol esters including the higher fatty acids such as palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid in addition to p ...
DOI:
10.1016/j.chroma.2014.03.021

25. Plant Sterol Diversity in Pollen from Angiosperms

Author:
Claire Villette; Anne Berna; Vincent Compagnon; Hubert Schaller
Source:
Lipids 2015 v.50 no.8 pp. 749-760
ISSN:
0024-4201
Subject:
Nicotiana tabacum; biogenesis; esters; flowering; gametophytes; males; mevalonic acid; phytosterols; pollen; pollen tubes; radiolabeling
Abstract:
... Here we have examined the composition of free sterols and steryl esters of pollen from selected angiosperm species, as a first step towards a comprehensive analysis of sterol biogenesis in the male gametophyte. We detected four major sterol structural groups: cycloartenol derivatives bearing a 9β,19-cyclopropyl group, sterols with a double bond at C-7(8), sterols with a double bond at C-5(6), and ...
DOI:
10.1007/s11745-015-4008-x

26. Ecological Roles and Biological Activities of Specialized Metabolites from the Genus Nicotiana

Author:
Jassbi Amir Reza; Zare Somayeh; Asadollahi Mojtaba; Schuman Meredith C.
Source:
Chemical Reviews 2017 v.117 no.19 pp. 12227-12280
ISSN:
1520-6890
Subject:
Nicotiana tabacum; abiotic stress; alcohols; alkaloids; aromatic compounds; bioassays; biotic stress; business enterprises; diterpenoids; esters; flavonoids; genes; glycosides; herbivores; humans; industrial crops; medicinal properties; metabolites; models; molecular biology; pathogens; sesquiterpenoids; sugars; taxonomy; tobacco; trichomes
Abstract:
... Species of Nicotiana grow naturally in different parts of the world and have long been used both medicinally and recreationally by human societies. More recently in our history, Nicotiana tabacum has attracted interest as one of the most economically important industrial crops. Nicotiana species are frequently investigated for their bioactive natural products, and the ecological role of their spec ...
DOI:
10.1021/acs.chemrev.7b00001

27. Metabolic Flux Engineering of Cembratrien-ol Production in Both the Glandular Trichome and Leaf Mesophyll in Nicotiana tabacum

Author:
Hongying Zhang; Songtao Zhang; Yongxia Yang; Hongfang Jia; Hong Cui
Source:
Plant & cell physiology 2018 v.59 no.3 pp. 566-574
ISSN:
1471-9053
Subject:
Aphidoidea; Cauliflower mosaic virus; Nicotiana sylvestris; Nicotiana tabacum; bioengineering; biosynthesis; catalytic activity; cell growth; diploidy; enzymes; esters; gene expression; genome; gibberellins; leaves; mesophyll; microarray technology; roots; stems; sugars; tobacco; transcription (genetics); transcriptome; transgenic plants; trichomes
Abstract:
... Cembratrien-ol synthase (CBTS) catalyzes the first step in cembranoid biosynthesis, producing cembratrien-ols in plant trichomes. In our previous study, microarray transcriptomes between leaves with trichomes and leaves without trichomes showed that an NtCBTS2 gene was expressed exclusively and abundantly in trichomes. Here, two NtCBTS2 isogenes (NtCBTS2a and NtCBTS2b), derived from a diploid geno ...
DOI:
10.1093/pcp/pcy004

28. Accessing to the Nicotiana tabacum leaf antimicrobial activity: In-silico and in-vitro investigations

Author:
Yanelis Capdesuñer; Jorge García-Brizuela; Hans Peter Mock; Karel Vives Hernández; Martha Hernández de la Torre; Cosme E. Santiesteban-Toca
Source:
Plant physiology and biochemistry 2019 v.139 pp. 591-599
ISSN:
0981-9428
Subject:
Nicotiana tabacum; antimicrobial properties; antioxidant activity; artificial intelligence; chemical composition; diterpenoids; esters; leaves; models; phenotype; secondary metabolites; sugars; tobacco; trichomes
Abstract:
... In this research, in-silico and in-vitro approaches were adopted with the aim to investigate the relationship between the tobacco leaf structures (trichomes) and the production of secondary metabolites with antimicrobial activity. Machine learning techniques were used to know the correlation between phenotypic traits and the production of secondary metabolites in Nicotiana tabacum plants. Then, an ...
DOI:
10.1016/j.plaphy.2019.04.015