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... The genuine biotic elicitor from germ tube walls of Puccinia graminis f. sp. tritici (Pgt-elicitor) induces lignification, preceded by an increase in phenylalanine ammonia-lyase (PAL) activity, when injected into the intercellular space of primary wheat leaves. This increase in PAL activity is accompanied by an increase in other enzyme activities of the general phenylpropanoid pathway and the spec ...
random amplified polymorphic DNA technique; biomarkers; geographical variation; genetic distance; Puccinia graminis; genetic variation; isozymes; virulence; genetic markers; North America; Europe; South America
Abstract:
... Twenty isolates of Puccinia graminis f.sp. tritici from South America were compared with 19 from Europe using virulence, isozymes and random amplified polymorphic DNA (RAPD) markers. The isozyme and virulence patterns for these isolates were also compared with those of 11 isolates representative of the common race clusters in North America. All three types of marker showed a level of similarity be ...
... Stem rust race Pgt-QCCJ was first found in the Great Plains of the United States in 1989, collected primarily from barley. This race became a major part of the Puccinia graminis f. sp. tritici population, even though it is virulent to only a few hard red winter wheat cultivars in the central Great Plains and to barley in the northern Great Plains. It threatens barley production in the northern Gre ...
... A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt),as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after P ...
... Pathogenesis-related expression of the two antifungal hydrolases beta-1,3-glucanase (EC 3.2.1.39) and chitinase (EC 3.2.1.14) was studied in wheat (Triticum aestivum L.) as part of the defence response to stem rust (Puccinia graminis f.sp. tritici; Pgt), mediated by the semi-dominantly acting resistance genes Sr5 and Sr24. Complete resistance (infection type 0), mediated by the Sr5 gene in cultiva ...
... Near‐isogenic lines of the wheat (Triticum aestivum L.) cultivar Prelude, currying either the sr5 gene for susceptibility or the Sr5 allele for resistance to stem rust (Puccinia graminis Pers. f. sp. tritici Erics. & E. Henn.) were inoculated with race 32 of the fungus which possesses the P5 gene for avirulence. Enzyme extracts prepared at different times after inoculation were separated by non‐de ...