An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... KEY MESSAGE: Comparative sequence analysis was used to design a SNP marker that aided in the identification of new sources of oat stem rust resistance. New races of Puccinia graminis f. sp. avenae (Pga) threaten global oat production. An A. strigosa accession known to carry the broadly effective oat stem rust resistance gene, Pg6, was crossed with two susceptible A. strigosa accessions to generate ...
... Stem rust disease caused by the obligate biotrophic fungus Puccinia graminis f. sp. tritici significantly reduces the yield and quality of wheat grain. A total of 92 entries of new Siberian agricultural wheat germplasm were studied with an integrated approach: phenotyping in the field and seedling stage reactions and using molecular markers to identify stem rust resistance genes. Among the tested ...
... Thinopyrum intermedium (JJJˢJˢStSt, 2n = 6x = 42), a wild relative of common wheat, possesses many desirable agronomic genes for wheat improvement. The production of wheat–Thinopyrum intermedium introgression lines is a key step for transferring these beneficial genes into wheat. In this study, we characterized three wheat–Thinopyrum intermedium introgression lines TA3681, TA5566, and TA5567 using ...
... Oat stem rust, caused by Puccinia graminis f. sp. avenae, is one of the most devastating diseases of oat. The most cost-effective and environmentally friendly strategy to control this disease is the use of resistant cultivars. However, P. graminis f. sp. avenae can overcome the resistance of cultivars by rapidly changing its virulence. Thus, information on the virulence of P. graminis f. sp. avena ...
... Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a potentially devastating fungal disease of wheat worldwide. The present study was to evaluate the resistance of 42 wheat monogenic lines with known stem rust resistance (Sr) genes and 69 wheat cultivars to three new Pgt races (34C0MRGQM, 34C3MKGQM, and 34C6MTGSM) identified from aeciospores at the seedling and adult-plant stage ...
... KEY MESSAGE: A novel Ug99-resistant wheat–Thinopyrum ponticum translocation line was produced, its chromosomal composition was analyzed and specific markers were developed. Stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn (Pgt) has seriously threatened global wheat production since Ug99 race TTKSK was first detected in Uganda in 1998. Thinopyrum ponticum is near immune to Ug99 ...
... In recent years, leaf rust (LR) and stem rust (SR) have become a serious threat to bread wheat production in Kazakhstan. Most local cultivars are susceptible to these rusts, which has affected their yield and quality. The development of new cultivars with high productivity and LR and SR disease resistance, including using marker-assisted selection, is becoming an important priority in local breedi ...
Tyler Gordon; Yue Jin; Samuel Gale; Matthew Rouse; Samuel Stoxen; Ruth Wanyera; Godwin Macharia; Mandeep Randhawa; Sridhar Bhavani; Gina Brown-Guedira; David Marshall; Ebrahiem Babiker; Harold Bockelman; J. Michael Bonman
... Wheat stem rust caused by Puccinia graminis f. sp. tritici is a widespread and recurring threat to wheat production. Emerging P. graminis f. sp. tritici variants are rapidly overcoming major gene resistance deployed in wheat cultivars and new sources of race-nonspecific resistance are urgently needed. The National Small Grains Collection (NSGC) contains thousands of wheat landrace accessions that ...
Sachin Kumar; Tom G. Fetch; Ron E. Knox; Asheesh K. Singh; John M. Clarke; Ron M. Depauw; Richard D. Cuthbert; Heather L. Campbell; Davinder Singh; Sridhar Bhavani; Curtis J. Pozniak; Brad Meyer; Fran R. Clarke
... Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), has potential to cause major yield losses in durum wheat. Development of durum cultivars with improved resistance to stem rust is a sustainable option for effective control of this disease. This study was conducted to identify genomic regions associated with resistance to Ug99-derived Pgt races using a doubled haploid (DH) mapping popula ...
Puccinia graminis; Triticum aestivum; alleles; disease severity; haploidy; plant pathology; seedlings; spring wheat; stem rust; Canada; Kenya
Abstract:
... This study was conducted to identify and map stem rust resistance genes in Canada Western Red Spring (CWRS) wheat variety ‘AAC Prevail’ and elite line ‘BW961ʹ. A population of 227 doubled haploid lines from ‘AAC Prevail’/‘BW961ʹ and parents were evaluated for their response to stem rust (Puccinia graminis) at Njoro, Kenya, from 2016–2019 and genotyped using a targeted genotyping by sequencing SeqS ...
... Leaf (Puccinia triticina; Pt) and stem (Puccinia graminis tritici; Pgt) rust are important constraints to wheat production. The leaf and stem rust resistance in the Brazilian wheat varieties ‘Toropi’ and ‘BRS194’ was evaluated using a ‘Toropi/BRS194’ doubled-haploid (DH) population. Parents evaluated at the seedling stage with Pt race MCJ-HN displayed infection type (IT) = ;1 for ‘BRS194’ and IT = ...
... A diverse sexual population of wheat stem rust, Puccinia graminis f. sp. tritici, exists in the Pacific Northwest region of the United States because of the natural presence of Mahonia spp. that serves as alternate hosts to complete its sexual life cycle. The region appears to be a center of stem rust diversity in North America where novel virulence gene combinations can emerge that could overcome ...
... The production of malting barley under dryland and irrigated environments in South Africa (SA) has increased recently, making it the second most important winter crop after wheat. To assess the potential vulnerability of adapted barleys from SA to wheat stem rust (caused by Puccinia graminis f. sp. tritici, Pgt, race PTKST) and rye stem rust (Puccinia graminis f. sp. secalis, Pgs, isolate UVPgs1), ...
Puccinia graminis; Triticum aestivum; air temperature; chromosome mapping; chromosomes; emerging diseases; genetic resistance; genotyping by sequencing; phenotypic variation; plant disease resistance; plant pathogenic fungi; quantitative trait loci; resistance genes; seedlings; single nucleotide polymorphism; spring wheat; stem rust
Abstract:
... The identification and characterization of resistance genes should outpace the rapid emergence of new P. graminis f. sp. tritici races, such as TTRTF and TTKTT, to mitigate stem rust damage to wheat. The objective of the current study was to identify and characterize P. graminis f. sp. tritici race resistance association signals. A total of 250 North American spring wheat lines were evaluated at t ...
... Wheat stem rust, caused by the fungal pathogen Puccinia graminis f. sp. tritici (Pgt), has re-emerged as an important disease of wheat. This pathogen continues to spread and evolve new virulent races. The international letter-code system of nomenclature has been used to pathotype these new emerging races, and gene SrMcN from ‘McNair 701' winter wheat, which is one of the differential lines, was ex ...
... Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is one of the most serious fungal diseases in wheat production, seriously threatening the global supply of wheat and endangering food security. The present study was conducted to evaluate wheat monogenic lines with known Sr genes to the most prevalent P. graminis f. sp. tritici races in China. In addition, wheat lines introduced from the ...
... A wheat rust survey was conducted in Iraq in 2019 and collected 27 stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Erikks. & E. Henn.) samples. Seven samples were viable, and they were tested for races of P. graminis f. sp. tritici at the Regional Cereal Rust Research Center (RCRRC) in Izmir, Turkey, under strict quarantine procedures. Two 0.5-cm segments of each infected stem sh ...
Javier Hernandez; Alicia del Blanco; Tanya Filichkin; Scott Fisk; Lynn Gallagher; Laura Helgerson; Brigid Meints; Chris Mundt; Brian Steffenson; Patrick Hayes
... Stripe rust (incited by Puccinia striiformis f. sp. hordei) and stem rust (incited by P. graminis f. sp. tritici) are two of the most important diseases affecting barley. Building on prior work involving the introgression of the resistance genes rpg4/Rpg5 into diverse genetic backgrounds and the discovery of additional quantitative trait locus (QTLs) for stem rust resistance, we generated an array ...
... Most wheat cultivars planted worldwide are susceptible to the stem rust Ug99 race group. To prepare for the potential spread of these races into South America, we aimed to identify genomic regions responsible for resistance to Ug99 race group in germplasm adapted to South America. Two RIL populations from a cross between a stem rust susceptible parent “Baguette 13” and resistant local parents “INI ...
... Wheat stem rust (causal organism: Puccinia graminis f. sp. tritici) is an important fungal disease that causes significant yield losses in barley. The deployment of resistant cultivars is the most effective means of controlling this disease. Stem rust evaluations of a diverse collection of wild barley (Hordeum vulgare ssp. spontaneum) identified two Jordanian accessions (WBDC094 and WBDC238) with ...
... Known and unknown genes conferring seedling and adult plant resistance (APR) to leaf rust, stem rust and stripe rust were detected either singly or in combination in a set of 136 African wheat genotypes using multi‐pathotype tests with characterized Australian Puccinia triticina (Pt), P. graminis f. sp. tritici (Pgt) and P. striiformis f. sp. tritici (Pst) pathotypes. Lines Beladi 132, IYN 68/9.44 ...
... Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) is an important disease of bread wheat (Triticum aestivum L.) in South Africa (SA). The frequent emergence of new Pgt races with increased virulence remains a major constraint when genetic control is considered. Monitoring the occurrence, distribution, and pathogenicity of Pgt is essential to quickly detect and track new races. During the ...
Monsif Ur Rehman; Sam Gale; Gina Brown‐Guedira; Yue Jin; David Marshall; Lynda Whitcher; Sharon Williamson; Matthew Rouse; Javed Ahmad; Gulzar Ahmad; Irfan Ahmad Shah; Mahboob Ali Sial; Yahya Rauf; Atiq Ur Rehman Rattu; Javed Iqbal Mirza; Rick Ward; Majid Nadeem; Ghulam Ullah; Muhammad Imtiaz
... Stem rust is a major disease of wheat (Triticum aestivum L.) worldwide and new Puccinia graminis f. sp. tritici (Pgt) races including TTKSK (Ug99) pose a serious threat to wheat production. The protection of new varieties against Pgt races can be increased by identifying and combining several types of stem rust resistance genes (Sr). We screened a set of 707 wheat lines and cultivars against 11 Pg ...
Javier Hernandez; Brian J. Steffenson; Tanya Filichkin; Scott P. Fisk; Laura Helgerson; Brigid Meints; Kelly J. Vining; David Marshall; Alicia del Blanco; Xianming Chen; Patrick M. Hayes
... Stem rust (incited by Puccinia graminis f. sp. tritici) is a devastating disease of wheat and barley in many production areas. The widely virulent African P. graminis f. sp. tritici race TTKSK is of particular concern, because most cultivars are susceptible. To prepare for the possible arrival of race TTKSK in North America, we crossed a range of barley germplasm—representing different growth habi ...
L. Gao; E. M. Babiker; I. C. Nava; J. Nirmala; Z. Bedo; L. Lang; S. Chao; S. Gale; Y. Jin; J. A. Anderson; U. Bansal; R. F. Park; M. N. Rouse; J. M. Bonman; H. Bariana
... The wheat stem rust fungus, Puccinia graminis f. sp. tritici (Pgt), race TTKSK and related races pose a serious threat to world wheat production. Knowing the effectiveness of wheat stem rust resistance (Sr) genes against Pgt race TTKSK is fundamental in mitigating this threat through resistance breeding. Sr15 was previously identified as being ineffective against Pgt race TTKSK. Here, multirace di ...
Puccinia graminis; Triticum; adults; mature plants; phenotype; plant response; resistance genes; seedlings; stem rust; virulence; wheat; South Africa
Abstract:
... Traditional race phenotyping of cereal rusts is based on the pathogenicity of field isolates on seedlings using a set of differential host lines. These lines are regularly planted in the field, often as part of trap nurseries. In trap nurseries the primary objective is to assess the occurrence of isolates with virulence or avirulence on key resistance genes. Although it is generally accepted that ...
... In Ethiopia, breeding rust resistant wheat cultivars is a priority for wheat production. A stem rust epidemic during 2013 to 2014 on previously resistant cultivar Digalu highlighted the need to determine the effectiveness of wheat lines to multiple races of Puccinia graminis f. sp. tritici in Ethiopia. During 2014 and 2015, we evaluated a total of 97 bread wheat and 14 durum wheat genotypes agains ...
... Stem rust [causal organism: Puccinia graminis f. sp. tritici (Pgt)], stripe rust [Puccinia striiformis f. sp. tritici (Pst)], and leaf rust [Puccinia triticina (Pt)] are important fungal diseases of wheat in Central Asia and worldwide. Therefore, identification of seedling and adult plant resistance (APR) genes is of major importance for the national wheat breeding program in many countries. The o ...
Avena sativa; Puccinia graminis; cultivars; genetic markers; genetic variation; mature plants; oats; phenotype; races; seedlings; urediniospores; virulence; South Africa
Abstract:
... Puccinia graminis f. sp. avenae (Pga) samples were collected from cultivated oat (Avena sativa L.) in trial plots and commercial fields and from wild oat (Avena spp.) during the 2016 and 2017 seasons. Field samples were purified through selection of single pustule isolates and subsequent urediniospore increases using the susceptible cultivar Swan. For race phenotyping, a set of 12 international di ...
... Puccinia graminis f. sp. tritici (Pgt), the causal organism of stem rust, is of global importance across wheat‐growing countries. However, some epidemics commence without the obvious presence of ‘alternate’ or ‘green bridge’ hosts, suggesting urediniospores can survive in the absence of suitable host plants for many weeks. Testing a range of inert material types, including metals, plastics, fabric ...
... Stem rust of wheat (caused by Puccinia graminis f.sp. tritici) gained high international attention in the last two decades, but does not occur regularly in Germany. Motivated by a regional epidemic in 2013, we analysed 15 spring and 82 winter wheat cultivars registered in Germany for their resistance to stem rust at the seedling stage and tested 79 of these winter wheat cultivars at the adult‐plan ...
... Dasypyrum villosum is a wild relative of common wheat (Triticum aestivum L.) with resistance to Puccinia graminis f. tritici, the causal agent of stem rust, including the highly virulent race TTKSK (Ug99). In order to transfer resistance, T. durum–D. villosum amphiploids were initially developed and used as a bridge to create wheat–D. villosum introgression lines. Conserved ortholog set (COS) mark ...
Puccinia graminis; barley; breeding lines; cultivars; microsatellite repeats; pathogens; phenotype; races; seedlings; stem rust; virulence; wheat; South Africa
Abstract:
... Race 2SA55 of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici, was described for the first time during 2000 in South Africa. This race is of particular interest as it was the first local report of increased virulence towards barley cultivars by wheat stem rust. Using three original accessions of 2SA55 from the rust collection of the University of the Free State, nine single pustule ...
... Stem rust (caused by Puccinia graminis f. sp. tritici), leaf rust (P. triticina), and stripe rust (P. striiformis f. sp. tritici) rank among the most important diseases of wheat worldwide. The development of resistant cultivars is the preferred method of controlling rust diseases because it is environmentally benign and also cost effective. However, new virulence types often arise in pathogen popu ...
... Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) is one of the most important diseases of wheat in Ethiopia. It has a reputation to cause yield losses reaching up to 100% on susceptible cultivars. A study was conducted to evaluate the infection responses of 34 bread and 20 durum wheat lines at the seedling stage against four major stem rust races; TKTTF (Digalu race), TTKSK (Ug99 race), ...
... Stem rust resistance gene Sr2 is an important slow-rusting gene which has provided resistance against stem rust for many years. The Sr2 gene has durable resistance against all stem rust pathogens including the Ug99 group of races. It shows recessive inheritance and is linked with two phenotypic markers, Pseudo Black Chaff (PBC) and High Temperature-Induced Seedling Chlorosis (HTISC). However, dire ...
DNA; Puccinia graminis; crop production; disease outbreaks; ethanol; genotyping; greenhouses; hard red spring wheat; microsatellite repeats; phenotype; resistance genes; seedlings; single nucleotide polymorphism; stem rust; stems; urediniospores; virulence; Ecuador; Ethiopia; Manitoba; Middle East; Minnesota; Missouri; Ontario; Pakistan; Yemen
Abstract:
... Wheat stem rust is a devastating disease that has incited severe epidemics, resulting in extreme yield losses over the past century. Stem rust infection in plots of wheat line UC11075, known to carry the Sr38 resistance gene, was unusual and severe in February 2016 in a nursery at the Instituto Nacional de Investigaciones Agropecuarias Austro station near Cuenca, Ecuador. Stems with heavy infectio ...
... Wheat landrace CItr 15026 previously showed adult plant resistance (APR) to the Ug99 stem rust race group in Kenya and seedling resistance to Puccinia graminis f. sp. tritici races QFCSC, TTTTF, and TRTTF. CItr 15026 was crossed to susceptible accessions LMPG-6 and Red Bobs, and 180 double haploid (DH) lines and 140 recombinant inbred lines (RIL), respectively, were developed. The 90K wheat iSelec ...
Puccinia graminis; chromosome mapping; chromosomes; cultivars; doubled haploids; genes; genetic markers; microsatellite repeats; progeny; races; seedlings; single nucleotide polymorphism; spring wheat; stem rust; strontium; virulence; Africa; Canada; Mexico; United States
Abstract:
... Stem rust, caused by Puccinia graminis f. sp. tritici, is a destructive disease of wheat that can be controlled by deploying effective stem rust resistance (Sr) genes. Highly virulent races of P. graminis f. sp. tritici in Africa have been detected and characterized. These include race TRTTF and the Ug99 group of races such as TTKSK. Several Canadian and U.S. spring wheat cultivars, including the ...
... KEY MESSAGE: QTL conferring a 14–40% reduction in adult plant stem rust severity to multiple races of Pgt were found on chromosome 5H and will be useful in barley breeding. Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt) is an important disease of barley. The resistance gene Rpg1 has protected the crop against stem rust losses for over 70 years in North America, but is not effective ag ...
... Wheat landrace PI 177906 has seedling resistance to stem rust caused by Puccinia graminis f. sp. tritici races TTKSK, TTKST, and BCCBC and field resistance to the Ug99 race group. Parents, 140 recombinant inbred lines, and 138 double haploid (DH) lines were evaluated for seedling resistance to races TTKSK and BCCBC. Parents and the DH population were evaluated for field resistance to Ug99 in Kenya ...
... The challenge posed by rapidly changing wheat rust pathogens, both in virulence and in environmental adaptation, calls for the development and application of new techniques to accelerate the process of breeding for durable resistance. To expand the resistance gene pool available for germplasm improvement, a panel of 159 landraces plus old cultivars was evaluated for seedling and adult plant resist ...
... Stem rust, caused by the fungus Puccinia graminis Pers. f. sp. tritici Ericks, is one of the most damaging diseases of wheat (Triticum aestivum L.). The recent emergence of the stem rust Ug99 race group poses a serious threat to world wheat production. Utilization of genetic resistance in cultivar development is the optimal way to control stem rust. Here, we report association mapping of stem rust ...
... KEY MESSAGE : Resistance to Ug99 stem rust in Triumph 64 was conferred by SrTmp on chromosome arm 6DS and was mapped to the same position as SrCad and Sr42 , however, the three genes show functional differences. Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is an important disease of wheat that can be controlled by effective stem rust resistance (Sr) genes. The emergence of virulent ...
... Many accessions of the wheat wild relative Sharon goatgrass (Aegilops sharonensis Eig., AES) are resistant to African races of the stem rust pathogen (i.e., Ug99 group races), which currently threaten wheat production worldwide. A procedure was designed to introgress the respective resistances to specific bread wheat genomes by producing plants homozygous for the A and B genomes and hemizygous for ...
... A mapping population of 126 doubled haploid (DH) lines derived from a cross between the English winter wheat cultivars Spark and Rialto was evaluated for response to Puccinia graminis f. sp. tritici in the greenhouse and in artificially inoculated field plots at two locations over 3 years (2011, 2012 and 2013). Genetic analysis indicated the involvement of two seedling genes (Sr5 and Sr31, contrib ...
Newcomb Maria; Olivera Pablo D.; Rouse Matthew N.; Szabo Les J.; Johnson Jerry; Gale Sam; Luster Douglas G.; Wanyera Ruth; Macharia Godwin; Bhavani Sridhar; Hodson David; Patpour Mehran; Hovmøller Mogens S.; Fetch Thomas G.; Jin Yue
... Frequent emergence of new variants in the Puccinia graminis f. sp. tritici Ug99 race group in Kenya has made pathogen survey a priority. We analyzed 140 isolates from 78 P. graminis f. sp. tritici samples collected in Kenya between 2008 and 2014 and identified six races, including three not detected prior to 2013. Genotypic analysis of 20 isolates from 2013 and 2014 collections showed that the new ...
... Genome‐wide association mapping is a powerful tool for dissecting the relationship between phenotypes and genetic variants in diverse populations. With the improved cost efficiency of high‐throughput genotyping platforms, association mapping is a desirable method of mining populations for favorable alleles that hold value for crop improvement. Stem rust, caused by the fungus Puccinia graminis f. s ...
... To determine stem rust resistance genes of wheat varieties in Yunnan province, 11 Chinese strains of Puccinia graminis f.sp. tritici with different virulence and 1 artificial mutant strain were used in 110 wheat varieties. The results indicated that among the 45 Sr genes, Sr31, Sr5, SrTmp, Sr30, Sr36, Sr8a, Sr11, Sr24, Sr29, Sr34, Sr9e, Sr26, Sr38, Sr47, and SrTt3 were characterised in 55 wheat va ...
... Stem rust is one of the most devastating diseases of wheat. Widely virulent races of the pathogen in the Ug99 lineage (e.g., TTKSK) are threatening wheat production worldwide; therefore, there is an urgent need to enhance the diversity of resistance genes in the crop. The objectives of this study were to identify new sources of resistance in wheat-alien introgression derivatives from Secale cereal ...