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in vivo studies; sexual maturity; liver function; protein synthesis; tissue repair; RNA; zinc; sucrose; nutrient deficiencies; homeostasis; dietary minerals; uridine
... A procedure is described for the quantitative estimation of ribonucleic acid phosphorus in soil. Organic phosphorus was extracted from soil by digestion with 1 N NaOH for 19 hours at 30°C. Acidification of the alkaline extract resulted in precipitation of organic materials or humus. The soluble organic phosphorus in the acid extract was separated from excess salt and concentrated by adsorption on ...
DNA; RNA; Rhode Island Red; animal pathology; body weight; chickens; chicks; hatching; liver; peanut meal; peanuts; poisoning; toxicity; vitamin A
Abstract:
... Rhode Island Red chicks were fed from hatching to 8 weeks of age a commercial ration to which was added a highly toxic groundnut meal; groups were killed at regular intervals for biochemical and histological examinations. Growth was severely retarded and the liver/body weight ratio was increased compared with control chicks. Increased hepatic fat, reduced vitamin A storage and fluctuation in the R ...
... Nucleoproteins and nucleic acids of apparently high quality can be prepared from maize seedlings by simple modifications of saline extraction and chloroform deproteinization procedures used to isolate transforming principles in bacteria. Seedlings are disintegrated by brief blending (2 minutes) with saline-EDTA-sodium lauryl sulfate; the mash is strained, brought to 1M with sodium perchlorate, and ...
... RNase-sensitive pyroninophilia has been demonstrated in neoplastic and non-neoplastic canine mast cell granules and it was concluded that these granules contain RNA. When combined with (1) the observed morphologic similarities between mast cells and plasma cells, (2) the presence of common distinctive ultrastructures, (3) the differentiation of both cells from a common precursor and (4) the partic ...
DNA; Medicago sativa; RNA; alfalfa; cold; cold tolerance; microsomes; temperature
Abstract:
... Investigations were conducted to evaluate the effects of temperature and photoperiod on metabolic changes during development and maintenance of cold hardiness of two alfalfa (Medicago sativa L.) varieties varying widely in inherent cold hardiness. Cold temperatures appeared to be of primary importance for development and maintenance of cold hardiness, whereas both temperature and pbotoperiod playe ...
RNA; algae; animals; bacteria; carbon; chlorophyll; ecosystems; hot springs; models; national parks; photosynthesis; primary productivity; quantitative analysis; temperature
Abstract:
... Quantitative measurements of photosynthesis (by carbon—14) and chlorophyll were made for algal mats which had developed at a series of stations along the thermal gradient formed by the outflow channel of a hot spring at Yellowstone National Park. The temperature range studied was from 30°—70°. Comparisons were made of photosynthetic efficiency, which had been measured and calculated in different w ...
... Microsomal metabolism of DDT is more active in housefly strains showing resistance to DDT. Susceptible as well as resistant strains produce two fairly polar metabolites in vitro (metabolites 2 and 3), and a water-soluble metabolite (metabolite 1). Resistance to DDT in one of the strains studied (Fe) is apparently brought about by the production of large amounts of metabolite 1 in vivo and not by D ...
... The cutaneous lesions in 25 spontaneous cases of porcine exudative epidermitis (EE) were studied. During the first 4 days mild acanthosis, increase of cytoplasmic RNA, enlarged nucleoli, and intercellular oedema in the stratum spinosum with formation of vesicles occur. A PAS-positive, diastase-resistant material accumulates in the stratum intermedium. The keratohyaline granules disappear and parak ...
... The effects of a pathogenic canine herpesvirus were studied sequentially in established canine thyroid adenocarcinoma tissue culture cells. The lesions were defined as early, middle, and late manifestations of viral effects based on the time a lesion first appeared. The early phase (3 to 10 hours) consisted of nucleolar swelling followed by disruption. The nucleolar changes were characterized by l ...
... Fixed and sectioned principal salivary glands of Myzus persicae stained with acridine orange at different pH‐values (pH 2.6 − 7.0) were investigated under the fluorescence microscope. There were 4 groups of cells, which differed in their ability to absorb acridine orange (Abb. 1). By histochemical demonstration of different substrates a characterization of those 4 groups was possible: 1 Cells A an ...
... Cytoplasmic inclusions in urinary bladder epithelium found in nine of 155 Rhesus monkeys were examined. The inclusions were brightly eosinophilic in HE-stained sections. In the electron microscope the inclusions appeared as bundles of filaments. They were not associated with viral particles, were not membrane-bound, and appeared to have a continuity with normal tonofilaments. They apparently conta ...
... Turnip yellow mosaic virus RNA and capsids, obtained by dissociation of the virus in 8 m-urea, 1 m-NaCl, pH 7 buffer may be reassociated into ribonucleoprotein complexes by dialysis against low pH, low ionic strength buffers, if polyvalent cations are present. These particles are not true virions, as they dissociate again at neutral pH. ...
Escherichia coli; RNA; absorbance; activation energy; electrophoresis; ions; magnesium; temperature
Abstract:
... The kinetics of renaturation of denatured 5-S RNA from Escherichia coli have been studied by estimating the composition of reaction mixtures from absorbance profiles of stained zones containing the two forms separated by polyacrylamide-gel electrophoresis. The kinetics are first order and proceed to completion irrespective of temperature or Mg²⁺ ion concentration, but they depend strongly on tempe ...
zinc; nutrient deficiencies; brain; young animals; animal development; RNA; chemical concentration; polyribosomes; suckling; animal growth; protein metabolism; rats
Abstract:
... A deficiency of dietary zinc during the suckling period of the rat results in a depression in normal growth, which is, in part, due to the inanition experienced by the dam. The pups from such zinc-deficient dams have smaller forebrains at all of the time intervals investigated in comparison with pups from both zinc-adequate controls. The cell number of the forebrain of the zinc-deficient pup was a ...
... The LD50's in mice of citrinin, ochratoxin A, and penicillic acid injected intraperitoneally were 89, 22, and 100 mg per kg of body weight, respectively. Paired combinations of the mycotoxins, citrinin:ochratoxin A (CI:OA), ochratoxin A:penicillic acid (OA:PA), and penicillic acid:citrinin (PA:CI) elicited synergistic lethal responses. After administration of citrinin, 14C orotic acid incorporatio ...
... In attached cotyledons of Cucurbita pepo L. protein, chlorophyll, RNA, DNA and carbohydrates were found to increase to a maximum level 14 days after commencement of germination, followed by a sharp decline thereafter. Cotyledons excised and planted in a nutrient solution 14 days after sowing showed maximum levels of protein, chlorophyll, RNA, DNA and soluble and insoluble sugars 52 days after plan ...