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Ranavirus; frogs; infectious diseases; liver; salamanders and newts
Abstract:
... Amphibian populations have been undergoing declines on a global scale. Among the many threats to these populations are emergent infectious diseases (EIDs). The Ranavirus in particular has been found within many declining amphibian populations. Although non-lethal sampling methods exist for some amphibian groups, such as salamanders, the anurans are traditionally tested using a lethal method. By co ...
Ranavirus; artificial selection; coevolution; emerging diseases; human health; humans; indigenous species; pathogens; phylogeny; salamanders and newts; trees; virulence; wildlife; North America
Abstract:
... Distinguishing whether pathogens are novel or endemic is critical for controlling emerging infectious diseases, an increasing threat to wildlife and human health. To test the endemic vs. novel pathogen hypothesis, we present a unique analysis of intraspecific host-pathogen phylogenetic concordance of tiger salamanders and an emerging Ranavirus throughout Western North America. There is significant ...
... 1. The hypothesis that habitat fragmentation (biotic or abiotic) alters the transmission of disease within a population is explored using field data from a well-studied amphibian-pathogen system. 2. We used the Ambystoma tigrinum-A. tigrinum virus (ATV) model system to show how habitat fragmentation as a result of emergent vegetation and habitat management affects disease transmission dynamics in ...
disease diagnosis; salamanders and newts; viral diseases of animals and humans; nematode infections; introduced species; mixed infection; Rhabditidae; captive animals; pandemic; pathogen identification; nucleotide sequences; distress; Ranavirus; signs and symptoms (animals and humans); animal diseases; Netherlands; Belgium
Abstract:
... A mass die-off of imported red tailed knobby newts (Tylototriton kweichowensis) occurred in 2004 in Belgium and the Netherlands. In addition to massive infection with Rhabdias tokyoensis, Ranavirus was isolated from three dead newts examined virologically and the gene coding for the major capsid protein of the virus was sequenced. The isolate showed 99.8% similarity to the published sequence of fr ...
Caudata; pathological processes and conditions; salamanders and newts; immunohistochemistry; viral diseases of animals and humans; Anura; emerging diseases; Ranavirus; toads; animal diseases; Spain
Abstract:
... This report describes the isolation and characterisation of the common midwife toad virus (CMTV) from juvenile alpine newts (Mesotriton alpestris cyreni) and common midwife toad (CMT) tadpoles (Alytes obstetricans) in the Picos de Europa National Park in Northern Spain in August 2008. A comparative pathological and immunohistochemical study was carried out using anti-CMTV polyclonal serum. In the ...
Ranavirus; anorexia; coat proteins; disease outbreaks; edema; electron microscopy; genes; head; hemorrhage; intestines; kidneys; light microscopy; liver; morbidity; mortality; necropsy; necrosis; salamanders and newts; sequence alignment; viruses; China
Abstract:
... From February to May 2010, an outbreak of disease occurred amongst farmed Chinese giant salamanders (Andrias davidianus) in Hanzhong County, Shanxi Province, China. Clinical signs included anorexia, lethargy, ecchymoses and swollen areas on the head and limbs, and skin ulceration. The aim of this study was to determine the cause of this disease. Necropsy examination revealed subcutaneous and intra ...
DNA; DNA-directed DNA polymerase; Iridovirus; Rana; Ranavirus; acidity; alkalinity; coat proteins; cytopathogenicity; cytoplasm; electron microscopy; head; necrosis; pH; polymerase chain reaction; salamanders and newts; turtles; virus replication; viruses; China
Abstract:
... In this study, we confirmed that a novel ranavirus was related to mass die-offs of farmed Chinese giant salamanders (Andrias davidianus) in Leshan, Sichuan Province, China. The farmed Chinese giant salamanders presented typical clinical symptoms that included ecchymoses or swollen areas on the head and limbs, necrosis of the limbs, and skin ulceration. Bacteriological and pathologic examinations f ...
Ranavirus; cytopathogenicity; endangered species; fish; frogs; major genes; mortality; nuclear localization signals; pathogens; phylogeny; physiological transport; salamanders and newts; translation (genetics); virulence; China
Abstract:
... Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic ...
Plethodontidae; Ranavirus; ecosystems; emerging diseases; fungi; pathogens; salamanders and newts; screening; surveys; watersheds; Appalachian region; Georgia; North Carolina
Abstract:
... The interactive effects of environmental stressors and emerging infectious disease pose potential threats to stream salamander communities and their headwater stream ecosystems. To begin assessing these threats, we conducted occupancy surveys and pathogen screening of stream salamanders (Family Plethodontidae) in a protected southern Appalachians watershed in Georgia and North Carolina, USA. Of th ...
Andrias; Ranavirus; nucleotide sequences; nucleotides; phylogeny; salamanders and newts; China
Abstract:
... A ranavirus (RV) was isolated from Chinese giant salamanders (Andrias davidianus) in China in 2010 and provisionally designated Andrias davidianus ranavirus (ADRV). The complete genome sequence is 106,719 nucleotides long. Genomic sequence and phylogenetic analyses showed that ADRV has a high degree of conservation with other RVs. ...
... Ranaviruses are emerging as serious pathogens across ectothermic taxa, recently causing mass die-offs including entire chelonian populations. Amphibians may serve as reservoirs for chelonian infections. To assess this idea and determine whether chelonians in the Midwest are infected with (or at risk for) ranavirus infections, we tested for presence of ranavirus infections among sympatric larval am ...
... A series of MHC alleles (including 26 class IA, 27 class IIA, and 17 class IIB) were identified from Chinese giant salamander Andrias davidianus (Anda-MHC). These genes are similar to classical MHC molecules in terms of characteristic domains, functional residues, deduced tertiary structures and genetic diversity. The majority of variation between alleles is found in the putative peptide-binding r ...
... The Chinese giant salamander, Andrias davidianus, is a nationally protected and cultured species in China. Recently, a severe epizootic occurred in cultured Chinese giant salamanders in Hubei, Hunan, Sichuan, Shaanxi, and Zhejiang provinces of China, causing substantial economic losses. The typical clinical signs of diseased larval animals were jaw and abdominal swelling and subcutaneous hemorrhag ...
Andrias; Ranavirus; T-lymphocytes; alternative splicing; antiviral properties; blood serum; cDNA libraries; complementary DNA; genes; immunoglobulin M; immunoglobulin Y; immunoglobulin heavy chains; salamanders and newts; surveys; thymus gland
Abstract:
... A ranavirus-induced thymus cDNA library was constructed from Chinese giant salamander, the largest extant amphibian species. Among the 137 putative immune-related genes derived from this library, these molecules received particular focus: immunoglobulin heavy chains (IgM, IgD, and IgY), IFN-inducible protein 6 (IFI6), and T cell receptor beta chain (TCRβ). Several unusual features were uncovered: ...
... Haemorrhagic disease of Chinese giant salamanders (Andrias davidianus) (CGSs) is an emerging condition caused by an iridovirus of the genus Ranavirus within the family Iridoviridae. Several studies have described different biological properties of the virus, but some aspects of its replication cycle, including ultrastructural alterations, remain unknown. The aim of the present study was to describ ...
Andrias; Iridovirus; Pimephales promelas; Ranavirus; Western blotting; antiviral properties; coat proteins; drugs; genes; liquid chromatography; neutralization; neutralization tests; proliferating cell nuclear antigen; proteomics; reverse transcriptase polymerase chain reaction; salamanders and newts; small interfering RNA; tandem mass spectrometry; toads; virion; China
Abstract:
... Chinese giant salamander iridovirus (CGSIV) is the emerging causative agent to farmed Chinese giant salamanders in nationwide China. CGSIV is a member of the common midwife toad ranavirus (CMTV) subset of the amphibian-like ranavirus (ALRV) in the genus Ranavirus of Iridoviridae family. However, viral protein information on ALRV is lacking. In this first proteomic analysis of ALRV, 40 CGSIV viral ...
Andrias; Cyprinidae; Ranavirus; chromatin; cytopathogenicity; electron microscopy; fish; nuclear membrane; pathogens; salamanders and newts; spleen; vacuoles; virion
Abstract:
... Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe disease in Chinese giant salamanders, the largest extant amphibian in the world. A fish cell line, Epithelioma papulosum cyprinid (EPC), and a new amphibian cell line, Chinese giant salamander spleen cell (GSSC), were infected with ADRV and observed by light and electron microscopy. The morphological changes in th ...
... Ferritin, an evolutionarily conserved iron-binding protein, plays important roles in iron storage and detoxification and in host immune response to invading stimulus as well. In the present study, we identified three ferritin subunit analog cDNAs from Chinese giant salamander (Andrias davidianus). All the three ferritin subunit cDNAs had a putative iron responsive element in the 5′-untranslated re ...
Plethodontidae; Ranavirus; aquatic environment; body condition; drought; life history; national parks; pathogens; salamanders and newts; Great Smoky Mountain region; United States
Abstract:
... Emerging pathogens are a potential contributor to global amphibian declines. Ranaviruses, which infect ectothermic vertebrates and are common in aquatic environments, have been implicated in die-offs of at least 72 amphibian species worldwide. Most studies on the subject have focused on pool-breeding amphibians, and infection trends in other amphibian species assemblages have been understudied. Ou ...
Pieter T. J. Johnson; Dana M. Calhoun; Amber N. Stokes; Calvin B. Susbilla; Travis McDevitt‐Galles; Cheryl J. Briggs; Jason T. Hoverman; Vasyl V. Tkach; Jacobus C. de Roode
... Classical research on animal toxicity has focused on the role of toxins in protection against predators, but recent studies suggest these same compounds can offer a powerful defense against parasites and infectious diseases. Newts in the genus Taricha are brightly coloured and contain the potent neurotoxin, tetrodotoxin (TTX), which is hypothesized to have evolved as a defense against vertebrate p ...