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... Indole is a popular and functional scaffold existing widely in the fields of medicine, pesticides, spices, food and feed additives, dyes, and many others. Among indoles, 2-arylindole represents a particular and interesting subset but has attracted less attention for drug discovery. In this study, we report a general, practical one-pot assembly of a variety of 2-arylindole derivatives. To develop n ...
... In northern China, the soil-born diseases of wheat have been getting more and more serious under a new farming system that returns maize straw to the field. In order to investigate the allelopathy of the decomposed maize straw products on three soil-born diseases of wheat, culture dish and pot experiments were conducted and the compounds in the products were identified by gas chromatography-mass s ...
... Aureobasidium pullulans and Sporobolomyces roseus are a saprotrophic yeasts fungi commonly found on the leaves of winter wheat and on wheat kernels. The objective of this study was to compare the inhibitory effects of two species of yeasts fungi, Aureobasidium pullulans var. pullulans (de Bary) G. Arnaud and Sporobolomyces roseus Kluyver & van Niel, on the causal agents of stem base diseases, Rhiz ...
... Rhizoctonia cerealis is a worldwide soil-borne pathogenic fungus that significantly infects wheat and causes sharp eyespot in China. However, the biocontrol strains used for the control of Rhizoctonia cerealis are insufficient. In the present study, antagonistic strain B1302 from the rhizosphere of wheat were isolated and identified as Bacillus mojovensis based on their morphological, physiologica ...
... PURPOSE: Wheat sheath blight mainly infected by Rhizoctonia cerealis is one of the soil-borne fungal diseases of wheat worldwide and prevalent in major wheat growing areas in China at present. This study aimed to evaluate the antifungal activity of 163 endophytic fungi on R. cerealis. Antifungal strains were identified and their biosynthetic potential was analysed. METHODS: The antifungal activity ...
Rhizoctonia cerealis; antifungal properties; boscalid; flutolanil; in vitro studies; mutants; mycelium; parents; risk; sclerotia; succinate dehydrogenase (quinone); virulence; wheat; China
Abstract:
... During 2010–2012, a total of 120 isolates of Rhizoctonia cerealis were collected from wheat with symptoms of sharp eyespot in four provinces (Henan, Shandong, Anhui and Jiangsu) in China. All the isolates were determined for baseline sensitivity to thifluzamide, a succinate dehydrogenase inhibitor (SDHI) with strong antifungal activity. The sampled pathogenic populations, never exposed to SDHIs, h ...
Botrytis cinerea; Cryphonectria parasitica; Rhizoctonia cerealis; Serratia; Triticum aestivum; Valsa sordida; antibiotics; antifungal properties; biological control; biological control agents; cell communication; chitinase; endophytes; homoserine; host plants; indole acetic acid; lactones; liquid chromatography; mechanism of action; plant diseases and disorders; plant pathogens; proteinases; quorum sensing; ribosomal DNA; siderophores; stems; synergism; tandem mass spectrometry
Abstract:
... Biological control is being considered as an alternative or a supplemental way of reducing the use of chemicals in agriculture. An endophytic strain G3 with potential as a biocontrol agent was isolated from the stems of Triticum aestivum L. It was classified by 16S rDNA sequencing as a member of Serratia. Strain G3 displayed a broad spectrum of antifungal activity in vitro against a number of phyt ...
... In this study, the changes in activities of peroxidase (POD), glutathione S-transferase (GST), catalase (CAT), and superoxide distmuase (SOD) in leaves of four wheat cultivars, including resistant cultivars Shannong 0431 and Shanhongmai, a mild-susceptible cultivar Lumai 21, and a susceptible cultivar Wenmai 6, were investigated to explore if these antioxidant-related enzymes were involved in whea ...
... This study details the isolation and characterisation of Pseudomonas chlororaphis subsp. aurantiaca strain Pa40, and is the first to examine P. chlororaphis for use in suppression of wheat sharp eyespot on wheat. Pa40 was isolated during an investigation aimed to identify biocontrol agents for Rhizoctonia cerealis. Over 500 bacterial strains were isolated from the rhizosphere of infected wheat and ...
... Wheat sharp eyespot (WSE) caused by Rhizoctonia cerealis is a major soilborne fungal pathogen in winter wheat in China. The succinate dehydrogenase inhibitor (SDHI) fungicide thifluzamide has significant fungicidal activity against Rhizoctonia spp., but the sensitivity of R. cerealis and the efficacy of thifluzamide for the control of R. cerealis are unknown. One-hundred sixty-seven R. cerealis is ...
... Twenty-three isolates of Rhizoctonia spp. from agronomic crops and turfgrasses were characterized by cytological and pathological methods in order to establish the identity, pathogenicity, and virulence of Rhizoctonia spp. and anastomosis groups that occur on these hosts in Mississippi. Twelve isolates were identified as R. solani, including the five anastomosis groups (AGs) AG-1-IB, AG-2-2, AG-4, ...
... The current study was focused on fungi colonizing the stem bases of wheat. Particularly, fungal species with significant harmful effect on wheat were studied. The dynamics of the stem base colonization by these fungi over the growing season were observed, and the association between individual pathogenic fungi was also evaluated. In addition, the fungal leaf sheaths penetration, which can cause ph ...
... Antagonistic bacterium, Bacillus subtilis NJ-18, can biologically control several plant diseases. A combined strategy of wheat sharp eyespot control consisting of B. subtilis NJ-18 and fungicides was evaluated. Greenhouse and field tests were conducted to determine the effect of combining the B. subtilis NJ-18 with the fungicides flutolanil and difenoconazole for the control of wheat sharp eyespot ...
... BACKGROUND: Sheath blight is an important disease caused by Rhizoctonia cerealis that affects wheat yields worldwide. No wheat varieties have been identified with high resistance or immunity to sheath blight. Understanding the sheath blight resistance mechanism is essential for controlling this disease. In this study, we investigated the response of wheat to Rhizoctonia cerealis infection by analy ...
... We report here the presence of a novel double-stranded RNA (dsRNA) virus in an isolate (R0959) of the fungus Rhizoctonia cerealis, the causal agent of sharp eyespot of wheat in China. Sequence analysis showed that the dsRNA segment is 17,486 bp long and contains a single open reading frame (ORF) with the potential to encode a protein of 5,747 amino acids. The predicted protein contains conserved m ...
... On the basis of the scaffolds widely used in drug design, a series of novel spirooxindole derivatives containing hydantoin, thiohydantoin, urea, and thiourea moieties have been designed, synthesized, characterized, and first evaluated for their biological activities. The diastereoselectivity mechanism is proposed, and the systematic conformational analysis is performed. The bioassay results show t ...
... Because gossypol and hemigossypol show antiviral activity but are structurally complex, we designed and synthesized a series of structurally simpler phthalide and coumarin derivatives. The phthalide derivatives were synthesized by opening the naphthalene ring of hemigossypol, and the coumarin derivatives were synthesized by ring-opening reactions of the phthalide derivatives with the goal of inves ...
... Succinate dehydrogenase (SDH) is regarded as a promising target for fungicide discovery. To continue our ongoing studies on the discovery of novel SDH inhibitors as fungicides, novel pyrazole–thiazole carboxamides were designed, synthesized, and evaluated for their antifungal activity. The results indicated that compounds 9ac, 9bf, and 9cb showed excellent in vitro activities against Rhizoctonia c ...
... BACKGROUND: Succinate dehydrogenase (SDH) plays an important role in the Krebs cycle, which is considered as an attractive target for development of succinate dehydrogenase inhibitors (SDHIs) based on antifungal agents. Thus, in order to discover novel molecules with high antifungal activities, SDH as the target for a series of novel nicotinamide derivatives bearing substituted pyrazole moieties w ...
Blumeria graminis; Botrytis cinerea; Gaeumannomyces; Rhizoctonia cerealis; Sclerotinia sclerotiorum; X-radiation; antifungal properties; boscalid; carboxamides; computer simulation; cucumbers; electrospray ionization mass spectrometry; enzyme inhibitors; gray mold; hydrogen bonding; in vivo studies; mechanism of action; median effective concentration; mycelium; nuclear magnetic resonance spectroscopy; plant pathogenic fungi; powdery mildew; succinate dehydrogenase (quinone); triazoles; wheat
Abstract:
... Succinate dehydrogenase (SDH) is demonstrably one of the most important molecular targets in development of new fungicide. In our continuous efforts to discover novel SDH inhibitors, forty-two carboxamide derivatives containing 1,2,3-triazole ring were designed and synthesized, which were precisely characterized by 1H NMR, ESI-MS, elemental analysis and X-ray single-crystal diffraction. The compou ...
... Rhizoctonia cerealis E.P. van der Hoeven (anamorph of Ceratobasidium cereale D.I. Murray and Burpee), which causes sharp eyespot in wheat, is a major soilborne fungal pathogen that severely impairs yield and quality of winter wheat in China. Because the pathogen is difficult to identify and quantify in soil using conventional methods, a rapid and reliable method is needed to detect and quantify th ...
... KEY MESSAGE: A wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust, sharp eyespot and high kernel number per spike was developed and characterized by molecular cytogenetic method as novel resistant germplasm. Rye (Secale cereale L.), a close relative of common wheat, is an important and valuable gene donor with multiple disease resistance for wheat improve ...
... Sharp eyespot, caused by Rhizoctonia cerealis, has become one of the most severe diseases affecting global wheat production in recent decades. Quick and efficient screening methods are required to accelerate the development of cultivars for sharp eyespot resistance in wheat breeding. Here, a two-step colonized wheat kernels (TSCWK) method for the inoculation and classification of sharp eyespot res ...
... The progress of development of stem-base pathogens in crops of second winter wheat was plotted in nine experiments in three years. The amount of each pathogen present was determined by quantitative PCR. Where Tapesia yallundae was present in quantifiable amounts, it usually developed earlier than the other eyespot pathogen, T. acuformis. Both species were usually present in greater amounts on cult ...
... A series of novel genipin glycoside derivatives incorporating 11 glycosidic moieties at either the 1 or 10 position of genipin were designed and synthesized. These compounds exhibited moderate to excellent inhibitory activities against tobacco mosaic virus. Especially, the in vitro and in vivo activities of compounds 6e, 7c, 7d, 7f, 7h, and 7i were comparable to that of ribavirin. In particular, c ...
... To contribute molecular diversity for novel fungicide development, a series of novel thiazole carboxamides were rationally designed, synthesized, and characterized with the succinate dehydrogenase (SDH) as target. Bioassay indicated that compound 6g showed the similar excellent SDH inhibition as that of Thifluzamide with IC₅₀ of 0.56 mg/L and 0.55 mg/L, respectively. Some derivatives displayed imp ...
... The RAPD method (Random Amplification of Polymorphous DNA) was used to distinguish Tapesia acufonnis and T. yallundae isolates from other pathogens of wheat stem base diseases (Fusarium avenaceum, F. culmorum, F. nivale, Rhizoctonia cerealis and R. so/ani).Isolates of the fungi originated from infected wheat plants (Triticum aestivum L.) collected at various locations in the Czech Republic. Three ...
Rhizoctonia cerealis; chemical control; developmental stages; farming systems; fertilizer rates; field experimentation; fungicides; herbicides; mineral fertilizers; pesticide application; polymerase chain reaction; roots; sowing; stems; villages; winter wheat
Abstract:
... Effects of agronomic practices on the occurrence of sharp eyespot and Rhizoctonia spp. in winter wheat were determined in two field experiments. In Experiment 1, in the village of Osiny, a comparison was made of disease in different farming systems. The farming systems were: organic, integrated, conventional, and monoculture. In Experiment 2, in the village of Mochełek, the effects of different ch ...
... Sharp eyespot caused by Rhizoctonia cerealis was assessed in four winter wheat crops surveyed at four locations in north-central Poland in 2006–2009. At the four locations symptoms developed on 41.9–67.7 % of shoots of all plants (average of 4 years) and on 49–73.5 % of shoots of diseased plants (average of 4 years). Slight (category 1) disease was most frequent, occurring on 24.4–41.3 % of shoots ...
... Rhizoctonia cerealis is a major fungal pathogen of wheat that causes great yield losses in all wheat-growing regions of the world. The biocontrol agent Bacillus subtilis XZ18-3 was investigated for inhibiting R. cerealis growth in wheat. The results of the mycelial growth test showed that the sterile filtrate of B. subtilis XZ18-3 could significantly inhibit the mycelial growth of R. cerealis and ...
... The effect of Trichoderma atroviride, T. harzianum, T. longibrachiatum, Clonostachys rosea and Bacillus subtilis isolates applied to wheat seeds against Rhizoctonia cerealis disease of seedlings was investigated under controlled greenhouse conditions. Most Trichoderma isolates significantly reduced the incidence of disease compared with the infected control. Bacillus subtilis was also effective ag ...
... Streptomyces have been used extensively as the biocontrol agents due to their ability to produce various antimicrobial compounds, such as antibiotics and hydrolytic enzymes. Streptomyces lydicus strain A02, which was isolated from the soil of suburban forest field in Beijing (China), is capable of producing natamycin and has proved to be a potential biocontrol agent to several plant fungal disease ...
... Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N2-fixation and phosphate and potassium-solubi ...
... Rhizoctonia cerealis, Bipolaris sorokiniana, and Fusarium spp. are three wheat-damaging soil-borne fungal pathogens causing sharp eyespot, common root rot, and crown rot, respectively. This study aimed to develop a multiplex PCR (mPCR) system to simultaneously detect these fungal pathogens. The mPCR assay was performed on wheat seedlings collected from a field in the Shandong Province. Specific pr ...
... Rhizoctonia cerealis and Bipolaris maydis are fungal plant pathogens that cause enormous agricultural losses of wheat and maize. Bacillus amyloliquefaciens PEBA20 inhibited mycelial growth and spore germination of the pathogens and reduced fungal infections in wheat and maize, indicating its potential for application as a biocontrol agent. ...
... Fusarium head blight (scab), primarily caused by Fusarium graminearum, is a devastating disease of wheat (Triticum aestivum L.) worldwide. Wheat sharp eyespot, mainly caused by Rhizoctonia cerealis, is one of the major diseases of wheat in China. The defensin RsAFP2, a small cyteine-rich antifungal protein from radish (Raphanus sativus), was shown to inhibit growth in vitro of agronomically import ...
... A putative novel mitovirus was found in isolate R1084 of the fungus Rhizoctonia cerealis, the causal agent of sharp eyespot of wheat in China. The full genome sequence of the virus was determined and analyzed. The complete cDNA sequence is 3149 nucleotides long with 59.7 % A+T content. Using either the fungal mitochondrial or universal genetic code, the viral genome was found to contain a single l ...
... Wheat sharp eyespot, a disease mainly caused by soilborne fungus Rhizoctonia cerealis, is a threat to world wheat production. Wheat’s genetic resistance to sharp eyespot is a potential approach to reducing the application of fungicides and farming practice inputs. To identify the genetic basis of sharp eyespot resistance in Niavt14, a recombinant inbred line population comprising 215 F₈ lines from ...
... Sharp eyespot on wheat is caused by Rhizoctonia cerealis anastomosis group D subgroup I (AG-DI) and is an economically important stem-base disease of wheat in temperate regions worldwide. However, the understanding about the field population structure of R. cerealis is limited. In this study, the genetic structure of four wheat-infecting populations in China was investigated using six microsatelli ...
Rhizoctonia cerealis; Triticum; basidiospores; field experimentation; fungi; genes; genetic markers; genotype; loci; pathogens; population structure; spring; stems; wheat; Oregon
Abstract:
... Wheat sharp eyespot, caused by the soilborne basidiomycete fungus Rhizoctonia cerealis group D subgroup I (AG‐DI), is a common disease of wheat stems that has increased in global importance in recent years. The disease caused a severe and extensive epidemic throughout the Willamette Valley of Oregon in 2014 and has remained one of the most important wheat diseases in this region subsequently. The ...
... The necrotrophic fungus Rhizoctonia cerealis is the causal agent of devastating diseases of cereal crops including wheat (Triticum aestivum). We present a high-quality genome assembly of R. cerealis Rc207, a virulent strain causing wheat sharp eyespot. The assembly (56.36 Mb) is composed of 17.87% repeat sequences and 14,433 predicted protein-encoding genes. The Rc207 genome encodes a large and di ...
... Rhizoctonia cerealis (teleomorph Ceratobasidium cereale) is a soil-borne plant pathogenic fungus that can cause sharp eyespot in wheat or yellow patch in grasses. In this study, 21 new endornavirus genomes were obtained from five R. cerealis strains through the high-throughput sequencing of viral double-stranded RNA. Eighteen viruses were identified as Alphaendornavirus, and three viruses were ide ...
... BACKGROUND: Thifluzamide, a succinate dehydrogenase inhibitor (SDHI) fungicide, is a promising fungicide for controlling wheat sharp eyespot (WSE). WSE is caused by Rhizoctonia cerealis. Information on the resistance mechanism of this pathogen to thifluzamide remains unavailable. RESULTS: We used selective reculturing and UV mutagenesis to generate thifluzamide‐resistant mutants. Thifluzamide‐resi ...
... A wheat β-1,3-glucanase gene (TaGluD) was identified as a fungal defense candidate. Its transcript induction was more than 60-fold higher in a resistant wheat line, Shannong0431, than in a susceptible wheat line, Wenmai6, after infection with Rhizoctonia cerealis. The TaGluD protein was overexpressed as inclusion bodies in Escherichia coli. After refolding and purification, TaGluD with 1 unit of β ...
... Studies were conducted to assess the importance of soil-borne pathogens as impediments to the production of direct-drilled wheat following Kentucky bluegrass in rotation. Bluegrass sods were collected as cores from commercial seed fields in October (after open-field burning) and in December and March (after exposure to normal freezing and thawing), treated with glyphosate after a 2- to 3-week peri ...
... Wall-associated kinases (WAKs) play an important role in plant defense and development. Considerable progress has been made in understanding WAK genes in Arabidopsis thaliana. However, much less is known about these genes in common wheat. Here, we isolated a novel wheat WAK gene TaWAK5 from sharp eyespot disease-resistant wheat line CI12633, based on a differentially-expressed sequence identified ...
... As a consequence of the importance of crop cultivation in Bioregenerative Life Support System (BLSS), there is a necessity of protecting the plants from pathogenic diseases. Cave soil bacteria can be utilized as a source for isolating biocontrol bacteria that are suitable for plant growth in BLSS. Pseudochrobactrum kiredjianiae strain A4 (GenBank accession KT203923) is a gram-positive, short-rod s ...