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1. Characterization of enterohemorrhagic Escherichia coli from diarrhoeic patients with particular reference to production of Shiga-like toxin

Author:
Mohamed A. Amin; Hany R. Hashem; Heba S. El-Mahallawy; Ali A. Abdelrahman; Hoda M. Zaki; Marwa M. Azab
Source:
Microbial pathogenesis 2022 v.166 pp. 105538
ISSN:
0882-4010
Subject:
Shiga toxin; Shiga-like toxins; acute kidney injury; ampicillin; antibiotic resistance; cephalexin; ciprofloxacin; colitis; cytopathogenicity; diarrhea; enterohemorrhagic Escherichia coli; fimbriae; genes; genetic variation; gentamicin; hemolysis; hemolytic uremic syndrome; imipenem; intimin; morbidity; mortality; nitrofurantoin; pathogenesis; pathotypes; phylogeny; serotypes; tetracycline; vancomycin; virulence
Abstract:
... Enterohemorrhagic Escherichia coli (EHEC) is a subtype of pathogenic E. coli that causes diarrhea or hemorrhagic colitis in humans, which can progresses to hemolytic uremic syndrome (HUS), a leading cause of acute renal failure in children, and morbidity and mortality in adults. Stool samples (n = 273) of patients (1 day-40 years old) suffered from bloody diarrhea and abdominal cramps, were examin ...
DOI:
10.1016/j.micpath.2022.105538

2. Designing of a chimeric protein contains StxB, intimin and EscC against toxicity and adherence of enterohemorrhagic Escherichia coli O157:H7 and evaluation of serum antibody titers against it

Author:
Zahra Sadri Najafabadi; Shahram Nazarian; Mohammad Kargar; Farshid Kafilzadeh
Source:
Molecular immunology 2021 v.134 pp. 218-227
ISSN:
0161-5890
Subject:
B-lymphocytes; Escherichia coli O157; Shiga-like toxins; T-lymphocytes; Western blotting; antibodies; blood serum; codon usage; computer simulation; diarrhea; enteropathogens; epitopes; humans; immunogenicity; immunoinformatics; intimin; prediction; recombinant fusion proteins; toxicity; vaccines; virulence
Abstract:
... Enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain is known as one of the major human foodborne pathogens. Lack of effective clinical treatment for human diarrheal diseases confirms the need for vaccine production against enteric bacteria such as E.coli O157:H7. Shiga-like toxin (Stx), EscC, and Intimin are the main important virulent factors of this enteric pathogen. In the present study, a ...
DOI:
10.1016/j.molimm.2021.03.016

3. Isolation and molecular characterization of shigatoxigenic O157 and Non-O157 Escherichia coli in Raw Milk marketed in Chittagong, Bangladesh

Author:
Md. Kauser-Ul Alam; Nazmul Sarwar; Shireen Akther; Monsur Ahmad; Paritosh Kumar Biswas
Source:
Journal of dairying, foods & home sciences 2021 v.40 no.1 pp. 1-7
ISSN:
0976-0563
Subject:
Shiga-like toxins; Singapore; agar; antigens; enterohemorrhagic Escherichia coli; eosin; genes; hemolysins; hygiene; intimin; methylene blue; milk; milk production; polymerase chain reaction; public health; raw milk; risk; selective media; spoilage; virulence; Bangladesh
Abstract:
... Quality and microbial safety of milk is demanding day by day as it is considered as a host for pathogenic and spoilage microorganisms. In this study, isolation and molecular characterization of shigatoxigenic O157 and non-O157 Escherichia coli in raw milk marketed in Chittagong, Bangladesh were done on 186 raw milk samples in Bangladesh. MacConkey agar was initially used to screen for the presence ...
DOI:
10.18805/ajdfr.DR-178

4. Occurrence, pathotypes, and antimicrobial resistance profiles of diarrheagenic Escherichia coli strains in animal source food products from public markets in Mashhad, Iran

Author:
Neda Fallah; Mehran Ghaemi; Kiarash Ghazvini; Mehrnaz Rad; Abdollah Jamshidi
Source:
Food control 2021 v.121 pp. 107640
ISSN:
0956-7135
Subject:
Shiga-like toxins; animal-based foods; antibiotic resistance; diagnostic techniques; enteroinvasive Escherichia coli; enteropathogenic Escherichia coli; enterotoxigenic Escherichia coli; food contamination; frequency; gastrointestinal diseases; genes; guidelines; hamburgers; humans; incidence; intestinal microorganisms; markets; meat; occurrence; pathogens; pathotypes; prevalence; raw milk; sampling; strains; tetracycline; virulence; Iran
Abstract:
... Diarrheagenic Escherichia coli (DEC) strains are common pathogens that transmitted through the consumption of contaminated foods, and cause acute intestinal diseases in human. The present study was conducted on 300 samples including raw milk, meat and some of their products from November 2016 to October 2017. Microbiological and molecular diagnostic techniques were used to identification of DEC. A ...
DOI:
10.1016/j.foodcont.2020.107640

5. Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria, Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669, Isolated from Endangered Turtles (Clemmys guttata)

Author:
Seema G. Thomas; Maryah A. Glover; Anutthaman Parthasarathy; Narayan H. Wong; Paul A. Shipman; André O. Hudson
Source:
Microorganisms 2020 v.8 no.8 pp. -
ISSN:
2076-2607
Subject:
Aeromonas hydrophila; Citrobacter freundii; Clemmys guttata; Shiga-like toxins; antibiotic resistance genes; bacteria; biofilm; diarrhea; endangered species; medical equipment; multiple drug resistance; operon; pathogens; phylogeny; polyethylene; polypropylenes; protein subunits; regulatory proteins; reverse transcriptase polymerase chain reaction; sequence analysis; transactivators; turtles; virulence
Abstract:
... Aeromonas hydrophila RIT668 and Citrobacter freundii RIT669 were isolated from endangered spotted turtles (Clemmys guttata). Whole-genome sequencing, annotation and phylogenetic analyses of the genomes revealed that the closest relative of RIT668 is A. hydrophila ATCC 7966 and Citrobacter portucalensis A60 for RIT669. Resistome analysis showed that A. hydrophila and C. freundii harbor six and 19 d ...
DOI:
10.3390/microorganisms8081172

6. Production of egg yolk antibody (IgY) against shiga-like toxin (stx) and evaluation of its prophylaxis potency in mice

Author:
Javad Fathi; Firouz Ebrahimi; Shahram Nazarian; Abbas Hajizade; Yalda Malekzadegan; Ali Abdi
Source:
Microbial pathogenesis 2020 v.145 pp. 104199
ISSN:
0882-4010
Subject:
Escherichia coli O157; Shiga-like toxins; antibiotics; antibodies; bacterial adhesion; colitis; disease control; egg yolk; eggs; enteropathogens; enzyme-linked immunosorbent assay; epithelium; hemolytic uremic syndrome; immunoglobulin Y; intestines; mice; neutralization; pathogenicity; polyacrylamide gel electrophoresis; ribosomes; water pollution
Abstract:
... Enterohemorrhagic Escherichia coli (E. coli O157: H7) is an enteric pathogen, transmitted through contaminated water and food. Pathogenic factors include bacterial adhesion, invasion of intestinal epithelial and epithelium cells. The pathogenicity of EHEC is due to the production of Shiga-like toxin (Stx). This toxin binds to the ribosome and inhibits the synthesis of proteins. EHEC causes hemorrh ...
DOI:
10.1016/j.micpath.2020.104199

7. Magnetic resonance imaging appearance of the brain and cervical spinal cord in an edema disease affected pig

Author:
Lucía Dieste‐Pérez; Tetyda P. Dobak; Federico R. Vilaplana Grosso; Wilhelmina Bergmann; Tijs J. Tobias
Source:
Veterinary radiology & ultrasound 2019 v.60 no.2 pp. E15
ISSN:
1058-8183
Subject:
Escherichia coli; Shiga-like toxins; brain; cervical spine; edema; euthanasia; head; histopathology; magnetic resonance imaging; males; nervous system diseases; spinal cord; swine; toxicity
Abstract:
... A 7‐week‐old male pig was presented with signs of a central nervous system disorder. An MRI of the head and cervical spine was performed immediately after euthanasia. The MRI revealed multifocal bilaterally symmetric T2‐weighted hyperintense lesions in the brain and spinal cord, likely due to a toxic metabolic process. Histopathological examination supported the MRI findings and confirmed the diag ...
DOI:
10.1111/vru.12524

8. Optimized sample treatment, combined with real-time PCR, for same-day detection of E. coli O157 in ground beef and leafy greens

Author:
Alejandro Garrido-Maestu; Sarah Azinheiro; Joana Carvalho; Pablo Fuciños; Marta Prado
Source:
Food control 2019 pp. 106790
ISSN:
0956-7135
Subject:
DNA; Escherichia coli O157; Escherichia infections; Shiga-like toxins; bacteria; enzymatic treatment; green leafy vegetables; ground beef; quantitative polymerase chain reaction; serotypes; validation of alternative methods
Abstract:
... E. coli O157 is the most commonly identified serotype among Shiga like-toxin E. coli infections. Current reference methodologies to detect these bacteria are reliable but take several days to provide a definitive result, as they require selective enrichment, molecular detection of the bacteria, followed by plate confirmation. Even alternative methods need extensive sample enrichment in order to in ...
DOI:
10.1016/j.foodcont.2019.106790

9. Source tracking of Shiga-like toxin-producing Escherichia coli in the fresh vegetable production system of South India

Author:
Periasamy Pushpakanth; Zachariah John Kennedy; Dananjeyan Balachandar
Source:
Annals of microbiology 2019 v.69 no.9 pp. 885-893
ISSN:
1590-4261
Subject:
Escherichia coli O157; Shiga-like toxins; beets; cabbage; carrots; coliform bacteria; feces; foodborne illness; fresh produce; genes; hygiene; irrigation; microbiological quality; onions; parsley; pathogens; plate count; potatoes; quantitative polymerase chain reaction; rhizosphere; soil; vegetable growing; washing; water resources; India
Abstract:
... PURPOSE: Numerous outbreaks of foodborne diseases through fresh agricultural produce urge research to assess the source of entry of pathogens to the produce that compromise microbiological safety. In the present investigation, the entry of shiga-like toxin-producing Escherichia coli O157:H7 in a fresh vegetable production system was assessed by microbiological and molecular approaches. METHODS: Fi ...
DOI:
10.1007/s13213-019-01479-2

10. Peracetic Acid (PAA) Disinfection: Inactivation of Microbial Indicators and Pathogenic Bacteria in a Municipal Wastewater Plant

Author:
Silvia Bonetta; Cristina Pignata; Eugenio Lorenzi; Margherita De Ceglia; Lorenza Meucci; Sara Bonetta; Giorgio Gilli; Elisabetta Carraro
Source:
Water 2017 v.9 no.6 pp. -
ISSN:
2073-4441
Subject:
Campylobacter coli; DNA; Enterococcus; Escherichia coli O157; Salmonella; Shiga-like toxins; aquatic ecosystems; coliform bacteria; disinfectants; disinfection; effluents; genes; indicator species; irrigation; microbiological quality; municipal wastewater; peracetic acid; sewage; virulence; virulent strains; wastewater treatment
Abstract:
... Several studies have noted that treated and untreated wastewaters are primary contributors of a variety of pathogenic microorganisms to the aquatic ecosystem. Conventional wastewater treatment may not be sufficient to achieve microbiologically safe effluent to be discharged into natural waters or reused, thus requiring wastewater effluents to be disinfected. In recent years, peracetic acid (PAA) h ...
DOI:
10.3390/w9060427

11. Efficient and regioselective synthesis of globotriose by a novel α-galactosidase from Bacteroides fragilis

Author:
Wei Gong; Li Xu; Guofeng Gu; Lili Lu; Min Xiao
Source:
Applied microbiology and biotechnology 2016 v.100 no.15 pp. 6693-6702
ISSN:
0175-7598
Subject:
Bacteroides fragilis; DNA; Escherichia coli; Shiga-like toxins; alpha-galactosidase; amino acids; bacterial infections; epitopes; genes; glycosides; lactose; melibiose; molecular weight; neoplasms; pH; polysaccharides; temperature; therapeutics
Abstract:
... Globotriose (Galα1–4Galβ1–4Glc) is an important cell surface epitope that acts as the receptor for Shiga-like toxins, and it is also the core structure of Globo H and SSEA4 that are tumor-associated glycans. Hence, the enzymatic synthesis of globotriose would be necessary for the development of carbohydrate-based therapeutics for bacterial infections and cancers. Here, a novel GH27 α-galactosidase ...
DOI:
10.1007/s00253-016-7464-1

12. Enterohemorrhagic Escherichia coli (EHEC) in water from karst springs: detection with real-time PCR and isolation of strains

Author:
Andreas Baumgartner; Isabel Niederhauser; David Diston; Dominik Moor
Source:
Journal für Verbraucherschutz und Lebensmittelsicherheit 2016 v.11 no.4 pp. 353-357
ISSN:
1661-5751
Subject:
Shiga-like toxins; enterohemorrhagic Escherichia coli; food safety; foods; genes; intimin; karsts; quantitative polymerase chain reaction; springs (water); virulence; virulent strains; Switzerland
Abstract:
... Within 2 months, two water sources in a karst area in Switzerland were sampled 9 times each, and analyzed by real-time PCR for 6 EHEC O-types, Shiga-like-toxin (stx1 and stx2) and intimin (eae) genes. With the exception of O111, 5 O-types were recorded regularly and at high frequencies (O26: 33.3 %; O157: 33.3 %; O104: 66.6 %; O103: 72.2 %; O145: 94.4 %). Genes for Shiga-like-toxins and intimin we ...
DOI:
10.1007/s00003-016-1053-1

13. Influence of yogurt fermentation and refrigerated storage on the stability of protein toxin contaminants

Author:
Lauren S. Jackson; Odbert A. Triplett; William H. Tolleson
Source:
Food and chemical toxicology 2015 v.80 pp. 101-107
ISSN:
0278-6915
Subject:
Escherichia coli; Shiga-like toxins; acidity; adulterated products; cold storage; cytotoxicity; fermentation; food safety; manufacturing; microorganisms; pH; pasteurization; ready-to-eat foods; ricin; risk; skim milk; storage quality; toxicology; whole milk; yogurt
Abstract:
... Dairy products sold in a ready-to-eat form present the risk that adulterants persisting through manufacturing, storage, and distribution would reach consumers. Pathogenic microbes, including shigatoxigenic strains of Escherichia coli and the toxins they produce, are common food safety hazards associated with dairy products. Ricin and abrin are plant-derived ribosome-inactivating protein toxins rel ...
DOI:
10.1016/j.fct.2015.03.003

14. Safe and Effective Means of Detecting and Quantitating Shiga-Like Toxins in Attomole Amounts

Author:
Silva Christopher J.; Erickson-Beltran Melissa L.; Skinner Craig B.; Dynin Irina; Hui Colleen; Patfield Stephanie A.; Carter John Mark; He Xiaohua
Source:
Analytical chemistry 2014 v.86 no.10 pp. 4698-4706
ISSN:
1520-6882
Subject:
Shiga toxin-producing Escherichia coli; Shiga-like toxins; detection limit; mass spectrometry; mice; monitoring; peptides; protein subunits
Abstract:
... Shiga-like toxins (verotoxins) are a class of AB₅ holotoxins that are primarily responsible for the virulence associated with Shiga-like toxin producing Escherichia coli (STEC) infections. The holotoxins are composed of a pentamer of identical subunits (B subunit) responsible for delivering the catalytic subunit (A subunit) to a host cell and facilitating endocytosis of the toxin into the cell. Th ...
DOI:
10.1021/ac402930r

15. Bridging lectin binding sites by multivalent carbohydrates

Author:
Valentin Wittmann; Roland J. Pieters
Source:
Chemical Society reviews 2013 v.42 no.10 pp. 4492-4503
ISSN:
1460-4744
Subject:
Erythrina; Pseudomonas aeruginosa; Shiga-like toxins; X-ray diffraction; agglutinins; chelation; cholera toxin; concanavalin A; enterotoxins; ligands; viral antibodies; wheat germ
Abstract:
... Carbohydrate–protein interactions are involved in a multitude of biological recognition processes. Since individual protein–carbohydrate interactions are usually weak, multivalency is often required to achieve biologically relevant binding affinities and selectivities. Among the possible mechanisms responsible for binding enhancement by multivalency, the simultaneous attachment of a multivalent li ...
DOI:
10.1039/c3cs60089k

16. Magnetic Nanoparticle-Based Platform for Characterization of Histidine-Rich Proteins and Peptides

Author:
Huang Shin-Yi; Chen Yu-Chie
Source:
Analytical chemistry 2013 v.85 no.6 pp. 3347-3354
ISSN:
1520-6882
Subject:
Shiga-like toxins; desorption; detection limit; dissociation; humans; ionization; magnetic materials; matrix-assisted laser desorption-ionization mass spectrometry; mixing; nanoparticles; pH; peptides; proteins
Abstract:
... In this study, we developed a platform that can be used to rapidly enrich polyhistidine(His)-tagged proteins/peptides from complex samples selectively using the Fe₃O₄@Al₂O₃ magnetic nanoparticles (MNPs) as the affinity probes. At pH 7, the dissociation constant between poly-His, i.e., His₆, and the Fe₃O₄@Al₂O₃ MNPs was ∼10–⁵ M and the trapping capacity was ∼100 nmol/mg for His₆. Enrichment was ach ...
DOI:
10.1021/ac4000128

17. Dietary clays alleviate diarrhea of weaned pigs

Author:
M. Song; Y. Liu; J. A. Soares; T. M. Che; O. Osuna; C. W. Maddox; J. E. Pettigrew
Source:
Journal of animal science 2012 v.90 no.1 pp. 345-360
ISSN:
0021-8812
Subject:
Escherichia coli; Shiga-like toxins; agar; clay; coliform bacteria; diarrhea; diet; feces; hematocrit; kaolinite; lymphocytes; microbial growth; neutrophils; smectite; swine
Abstract:
... Two experiments were conducted to determine whether 3 different clays in the nursery diet reduce diarrhea of weaned pigs experimentally infected with a pathogenic Escherichia coli. Weaned pigs (21 d old) were housed in individual pens of disease containment chambers for 16 d [4 d before and 12 d after the first challenge (d 0)]. The treatments were in a factorial arrangement: 1) with or without an ...
DOI:
10.2527/jas.2010-3662
PubMed:
21908641

18. Effects of sugar addition in Luria Bertani (LB) media on Escherichia coli O157:H7

Author:
Medina, Marjorie B.; Uknalis, Joseph; Tu, Shu-I
Source:
Journal of food safety 2011 v.31 no.3 pp. 386-394
ISSN:
0149-6085
Subject:
Escherichia coli O157; Escherichia infections; Shiga toxin; Shiga-like toxins; bacteria; cell growth; chemistry; foods; glucose; hemolytic uremic syndrome; humans; lactose; metabolism; pathogenicity; quorum sensing
Abstract:
... Human pathogenic Escherichia coli O157:H7 produces Shiga-like toxins (SLT) that cause hemolytic uremic syndrome. Typically, SLT are released when a bacterium lyses, but the mechanism of controlling SLT production is not clearly understood. This paper studies the cell growth and metabolism of E. coli in Luria Bertani (LB) broth with and without added sugars. Sugars added to LB broth cause a variety ...
DOI:
10.1111/j.1745-4565.2011.00311.x

19. Micro-array for the identification of Shiga toxin-producing Escherichia coli (STEC) seropathotypes associated with Hemorrhagic Colitis and Hemolytic Uremic Syndrome in humans

Author:
Bugarel, Marie; Beutin, Lothar; Martin, Annett; Gill, Alexander; Fach, Patrick
Source:
International journal of food microbiology 2010 v.142 no.3 pp. 318-329
ISSN:
0168-1605
Subject:
enterohemorrhagic Escherichia coli; bacterial contamination; microarray technology; food analysis; food contamination; serotypes; genes; Shiga-like toxins; Shiga toxin; virulence; human diseases; hemolytic uremic syndrome; colitis
Abstract:
... A micro-array has been developed, based on the GeneDisc® array, for the genetic identification of 12 O-types and 7 H-types of Shiga toxin-producing Escherichia coli (STEC) including the most clinically relevant enterohemorrhagic E. coli (EHEC) serotypes. The genes selected for determination of the O antigens (rfbE O₁₅₇, wzx O₂₆, wzx O₁₀₃, wbd1 O₁₁₁, ihp1 O₁₄₅, wzx O₁₂₁, wzy O₁₁₃, wzy O₉₁, wzx O₁₀₄ ...
DOI:
10.1016/j.ijfoodmicro.2010.07.010
PubMed:
20675003

20. Monoclonal antibodies against Stx1B subunit of Escherichia coli O157:H7 distinguish the bacterium from other bacteria

Author:
Li, G.; Hong, J.; Huo, G.; Ren, X.
Source:
Letters in applied microbiology 2010 v.51 no.5 pp. 499-503
ISSN:
0266-8254
Subject:
Escherichia coli O157; Listeria monocytogenes; Mycobacterium tuberculosis; Salmonella; Shiga-like toxins; Staphylococcus aureus; Streptococcus agalactiae; Western blotting; bacteria; enteritis; enzyme-linked immunosorbent assay; hybridomas; monitoring; monoclonal antibodies; virulence
Abstract:
... The Shiga-like toxins (Stx) are critical virulence factors of enterohaemorrhagic Escherichia coli (EHEC). Stx1B subunit plays important roles in EHEC infection. This work aims to generate and characterize monoclonal antibodies (mAbs) against the Stx1B and to investigate their utility in discrimination ELISA. Two newly identified mAbs (designated 2H8 and 1B10, respectively) against the Stx1B protei ...
DOI:
10.1111/j.1472-765X.2010.02919.x
PubMed:
20854398

21. Pheno-genotypic characterisation of Escherichia coli O157:H7 isolates from domestic and wild ruminants

Author:
Sánchez, S.; Martínez, R.; Rey, J.; García, A.; Blanco, J.; Blanco, M.; Blanco, J.E.; Mora, A.; Herrera-León, S.; Echeita, A.; Alonso, J.M.
Source:
Veterinary microbiology 2010 v.142 no.3-4 pp. 445-449
ISSN:
0378-1135
Subject:
Shiga-like toxins; pulsed-field gel electrophoresis; phenotype; feces; genotype; wild animals; cattle; polymerase chain reaction; strain differences; animal pathogenic bacteria; pathogen identification; microbial genetics; Escherichia infections; disease reservoirs; toxigenic strains; sheep; disease transmission; Escherichia coli O157; goat milk; deer; Spain
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) O157:H7 represents a major public health concern worldwide, with ruminants recognised as their main natural reservoir. The aim of this work was to determine the phenotypic features and genetic relationships of 46 E. coli O157:H7 isolates obtained from sheep, cattle and deer faeces and from unpasteurised goat milk in Spain over a period of 11 years. Cha ...
DOI:
10.1016/j.vetmic.2009.10.009
PubMed:
19914011

22. Production of Shiga-like toxins in viable but nonculturable Escherichia coli O157:H7

Author:
Liu, Yanming; Wang, Chuan; Tyrrell, Gregory; Li, Xing-Fang
Source:
Water research 2010 v.44 no.3 pp. 711-718
ISSN:
0043-1354
Subject:
temporal variation; viability; Shiga-like toxins; inhibitory concentration 50; toxigenic strains; cell culture; cytotoxicity; strain differences; animal pathogenic bacteria; drinking water; pathogenicity; abiotic stress; Escherichia coli O157; cell lines; culture media
DOI:
10.1016/j.watres.2009.10.005
PubMed:
19879621

23. Variation in the prevalence of non-O157 Shiga toxin-producing Escherichia coli in four sheep flocks during a 12-month longitudinal study

Author:
Sánchez, S.; Martínez, R.; García, A.; Benítez, J.M.; Blanco, J.; Blanco, J.E.; Blanco, M.; Dahbi, G.; López, C.; Mora, A.; Alonso, J.M.; Rey, J.
Source:
Small ruminant research 2010 v.93 no.2-3 pp. 144-148
ISSN:
0921-4488
Subject:
Escherichia infections; Escherichia coli; Shiga-like toxins; sheep; flocks; longitudinal studies; seasonal variation; disease prevalence; virulence; Merino; Spain
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) are an important group of emerging pathogens, with ruminants recognised as their main natural reservoir. The aim of this longitudinal study was to provide information on the prevalence and existence of seasonal variation in the occurrence of non-O157 STEC in four sheep flocks over a 12-month period. A total of 504 faecal samples from 48 adult sheep in ...
DOI:
10.1016/j.smallrumres.2010.05.014

24. Virulence profile comparison between LEE-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and humans

Author:
Galli, Lucía; Miliwebsky, Elizabeth; Irino, Kinue; Leotta, Gerardo; Rivas, Marta
Source:
Veterinary microbiology 2010 v.143 no.2-4 pp. 307-313
ISSN:
0378-1135
Subject:
cattle diseases; Shiga-like toxins; Shiga toxin; interspecific variation; zoonoses; fimbriae; loci; biomarkers; serotypes; adhesins; virulence; disease severity; food pathogens; genetic variation; pathotypes; Escherichia infections; enterovirulent Escherichia coli group; Argentina
Abstract:
... For comparison purposes, the prevalence of 8 virulence markers was investigated, by PCR, in 153 cattle and 47 human Locus for Enterocyte Effacement (LEE)-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated in Argentina. Also, their correlation with severe disease was established. The virulence markers studied comprises 5 fimbrial and nonfimbrial adhesin-encoding genes (fimA, ih ...
DOI:
10.1016/j.vetmic.2009.11.028
PubMed:
20022185

25. Simultaneous detection of Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Escherichia coli O157:H7 in food samples using Multiplex PCR method

Author:
Zhang, D.; Zhang, H.; Yang, L.; Guo, J.; Li, X.; Feng, Y.
Source:
Journal of food safety 2009 v.29 no.3 pp. 348-363
ISSN:
0149-6085
Subject:
pork; Shiga-like toxins; Staphylococcus aureus; genes; fish; Salmonella enterica; bacterial contamination; detection limit; hemolysins; cabbage; Listeria monocytogenes; leaves; canola; monitoring; polymerase chain reaction; nucleases; food contamination; cheeses; food pathogens; beef; nucleotide sequences; shrimp; Escherichia coli O157; ground meat; intimin
Abstract:
... In this study, one multiplex polymerase chain reaction (MPCR) assay was developed for simultaneous detection of four foodborne pathogens, i.e., Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Escherichia coli O157:H7. Five specific primer pairs were designed based on the nucleotide sequences of hemolysin gene (hly) of Listeria monocytogenes, thermostable nuclease gene (nuc) ...
DOI:
10.1111/j.1745-4565.2009.00161.x

26. Activatable Shiga toxin 2d (Stx2d) in STEC strains isolated from cattle and sheep at slaughter

Author:
Tasara, Taurai; Bielaszewska, Martina; Nitzsche, Sabine; Karch, Helge; Zweifel, Claudio; Stephan, Roger
Source:
Veterinary microbiology 2008 v.131 no.1-2 pp. 199-204
ISSN:
0378-1135
Subject:
restriction fragment length polymorphism; disease reservoirs; Shiga-like toxins; slaughter; zoonoses; phenotype; alleles; sheep; cattle; adhesins; strain differences; animal pathogenic bacteria; feces; enterotoxigenic Escherichia coli; incidence; bacterial infections
Abstract:
... Shiga toxin producing Escherichia coli (STEC) harbouring the stx ₂d₋activatable gene and expressing the mucus- and elastase-activatable phenotype have been associated with severe outcomes of human disease. However, there is limited data available on the occurrence of such strains in livestock reservoirs. In this study, we analyzed 11 STEC strains isolated from healthy cattle and sheep at slaughter ...
DOI:
10.1016/j.vetmic.2008.03.001
PubMed:
18417300

27. Detection of Verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: A collaborative study

Author:
Hara-Kudo, Yukiko; Konishi, Noriko; Ohtsuka, Kayoko; Hiramatsu, Reiji; Tanaka, Hiroyuki; Konuma, Hirotaka; Takatori, Kosuke
Source:
International journal of food microbiology 2008 v.122 no.1-2 pp. 156-161
ISSN:
0168-1605
Subject:
Shiga-like toxins; bacterial contamination; sprouts (food); Escherichia coli O157; immunomagnetic separation; ground beef; collaborative testing; rapid methods; alfalfa; plate count; food pathogens; enrichment culture; microbial detection; virulence
Abstract:
... In order to establish a rapid and sensitive method for the detection of Verotoxigenic Escherichia coli O157 and O26, a collaborative study was conducted focusing on a comparison of the efficiency of loop-mediated amplification (LAMP) assay targeting the Verocytotoxin (also called Shiga toxin) gene, utilizing a direct plating method and a plating method with immunomagnetic separation (IMS-plating m ...
DOI:
10.1016/j.ijfoodmicro.2007.11.078

28. Diversity of virulence profiles of Shiga toxin-producing Escherichia coli serotypes in food-producing animals in Brazil

Author:
Oliveira, Murilo Gomes; Brito, José Renaldi Feitosa; Gomes, Tânia Aparecida Tardelli; Guth, Beatriz Ernestina Cabilio; Vieira, Mônica Aparecida Midolli; Naves, Zita Valéria Furtado; Vaz, Tânia Mara Ibelli; Irino, Kinue
Source:
International journal of food microbiology 2008 v.127 no.1-2 pp. 139-146
ISSN:
0168-1605
Subject:
Shiga-like toxins; bacterial contamination; toxigenic strains; serotypes; cytotoxicity; goats; beef cattle; virulence; genetic variation; dairy cattle; food pathogens; genes; Escherichia coli O157; sentinel animals; Brazil
Abstract:
... The prevalence, serotypes and virulence profiles of Shiga toxin-producing Escherichia coli (STEC) were investigated in 205 healthy beef and dairy cattle, and 106 goats reared in the southeastern region of Minas Gerais State, Brazil. The prevalence of STEC was 57.5% (61/106) in goats, 39.2%, (40/102) in beef cattle and 17.5% (18/103) in dairy cattle. Among the 514 STEC isolates, 40 different seroty ...
DOI:
10.1016/j.ijfoodmicro.2008.06.023
PubMed:
18678426

29. Effect of diet on the concentration of complex Shiga toxin-producing Escherichia coli and EHEC virulence genes in bovine faeces, hide and carcass

Author:
Gilbert, Rosalind Ann; Denman, Stuart Edward; Padmanabha, Jagadish; Fegan, Narelle; Al Ajmi, Dawood; McSweeney, Christopher S.
Source:
International journal of food microbiology 2008 v.121 no.2 pp. 208-216
ISSN:
0168-1605
Subject:
Shiga-like toxins; hides and skins; beef cattle; dietary carbohydrate; genes; feed grains; beef carcasses; bacterial contamination; molasses; feces; virulence; butyrates; slaughterhouses; feedlots; rumen fermentation; animal handling; high carbohydrate diet; experimental diets; food pathogens; pH; Escherichia coli O157; on-farm food safety
Abstract:
... An experiment was conducted to determine whether diets based on structural carbohydrate and/or simple sugars, as found in roughage and/or molasses-based diets, reduce the bovine faecal populations of Shiga toxin-producing Escherichia coli (STEC) isolates containing the eaeA and ehxA genes, referred to as complex STEC (cSTEC), compared with typical high starch, grain-based feedlot diets. In additio ...
DOI:
10.1016/j.ijfoodmicro.2007.11.019

30. Escherichia coli O157:H7 and other Shiga toxin-producing E. coli in white veal calves

Author:
Cristancho, L.; Johnson, R.P.; McEwen, S.A.; Gyles, C.L.
Source:
Veterinary microbiology 2008 v.126 no.1-3 pp. 200-209
ISSN:
0378-1135
Subject:
milk replacer; Shiga-like toxins; drug resistance; immunity; cattle diseases; protective effect; seroprevalence; risk assessment; feces; seroconversion; epidemiological studies; disease detection; Escherichia infections; diet; serodiagnosis; food animals; disease prevalence; disease transmission; food pathogens; Escherichia coli O157; anti-infective agents; veal calves; Ontario
Abstract:
... The aims of the study were to determine the prevalence of enterohemorrhagic Escherichia coli O157:H7 (EHEC O157) and other Shiga toxin-producing E. coli (STEC) in feces of white veal calves in an operation in Ontario, to evaluate exposure of the calves to EHEC O157, and to investigate the milk replacer diet and antimicrobial resistance as factors that might influence the prevalence of EHEC O157. F ...
DOI:
10.1016/j.vetmic.2007.06.012

31. Phylogenetic background of attaching and effacing Escherichia coli isolates from animals

Author:
Tramuta, C.; Robino, P.; Nebbia, P.
Source:
Veterinary research communications 2008 v.32 no.6 pp. 433-437
ISSN:
0165-7380
Subject:
Shiga-like toxins; alleles; diarrhea; serotypes; enterohemorrhagic Escherichia coli; enteropathogenic Escherichia coli; bacterial adhesion; phylogeny; virulence; molecular genetics; molecular epidemiology; intimin; pathotypes; Escherichia infections; animal diseases
Abstract:
... Detection and distribution of eae gene in forty-four attaching and effacing Escherichia coli (AEEC) strains of animal origin were investigated. Association of distinct intimin alleles with phylogenetic background were assessed among strains in comparison with different serogroups. Phylogenetic analysis showed that 31 EHEC/eae+ STEC strains belong to groups A, B1 and E, 13 EPEC strains segregated i ...
DOI:
10.1007/s11259-008-9042-1
PubMed:
18509740

32. Surveillance of shiga toxin-producing Escherichia coli in beef with effective procedures, independent of serotype

Author:
Hara-Kudo, Y.; Niizuma, J.; Goto, I.; Iizuka, S.; Kaji, Y.; Kamakura, K.; Suzuki, S.; Takatori, K.
Source:
Foodborne pathogens & disease 2008 v.5 no.1 pp. 97-103
ISSN:
1556-7125
Subject:
Shiga-like toxins; bacterial contamination; serotypes; food contamination; selective media; rapid methods; food pathogens; beef; microbial detection; genes; virulence; Escherichia coli
DOI:
10.1089/fpd.2007.0031
PubMed:
18260820

33. The immunogenicity of fusion protein linking the carboxyl terminus of the B subunit of Shiga toxin 2 to the B subunit of E. coli heat-labile enterotoxin

Author:
Ran, X.Q.; Wang, H.Z.; Liu, J.J.; Li, S.; Wang, J.F.
Source:
Veterinary microbiology 2008 v.127 no.1-2 pp. 209-215
ISSN:
0378-1135
Subject:
enterotoxins; in vivo studies; Shiga-like toxins; diarrhea; swine diseases; disease control; vaccine adjuvants; mice; subunit vaccines; bacterial antigens; bacterial proteins; edema; vaccination; vaccine development; immune response; Escherichia infections; piglets; in vitro studies; protective effect
Abstract:
... To augment the immunogenicity of the subunit B of Shiga toxin (Stx2e B) produced by Escherichia coli and protect piglets from edema disease in china, a fusion gene was constructed consisting of Stx2e B genetically linked at the N-terminus of the B subunit of heat-labile enterotoxin (LTB) in a translational fusion. After being induced with IPTG, the expressed fusion protein of Stx2e B-LTB was about ...
DOI:
10.1016/j.vetmic.2007.08.021

34. Antimicrobial susceptibility of Shiga toxin-producing Escherichia coli isolated from organic dairy farms, conventional dairy farms, and county fairs in Minnesota

Author:
Cho, S.; Fossler, C.P.; Diez-Gonzalez, F.; Wells, S.J.; Hedberg, C.W.; Kaneene, J.B.; Ruegg, P.L.; Warnick, L.D.; Bender, J.B.
Source:
Foodborne pathogens & disease 2007 v.4 no.2 pp. 178-186
ISSN:
1556-7125
Subject:
dairy cows; sulfamonomethoxine; Shiga-like toxins; tetracycline; bacterial contamination; agricultural exhibitions; antibiotic resistance; multiple drug resistance; animal pathogenic bacteria; food pathogens; Holstein; dairy farming; Escherichia coli O157; organic production; Minnesota
Abstract:
... This study compared the antimicrobial susceptibility of Shiga toxin-producing Escherichia coli (STEC) isolates from organic dairy farms, conventional dairy farms, and Minnesota county fairs. A total of 83 STEC isolates (43 O157 and 40 non-O157 STEC) were tested for antimicrobial susceptibility as determined by the automated broth microdilution method. Resistance to tetracycline was identified in 1 ...
DOI:
10.1089/fpd.2006.0074
PubMed:
17600485

35. Attachment of Shiga toxigenic Escherichia coli to stainless steel

Author:
Rivas, L.; Fegan, N.; Dykes, G.A.
Source:
International journal of food microbiology 2007 v.115 no.1 pp. 89-94
ISSN:
0168-1605
Subject:
microscopy; Shiga-like toxins; bacterial contamination; food surfaces; toxigenic strains; food processing equipment; bacterial adhesion; food pathogens; nontoxigenic strains; stainless steel; Escherichia coli O157
Abstract:
... Shiga toxigenic Escherichia coli (STEC) are important foodborne pathogens causing gastrointestinal disease worldwide. Bacterial attachment to food surfaces, such as stainless steel may lead to cross contamination of foods and subsequent foodborne disease. A variety of STEC isolates, including E. coli O157:H7/H- strains, were grown in planktonic (broth) and sessile (agar) culture, following which i ...
DOI:
10.1016/j.ijfoodmicro.2006.10.027

36. Isolation and characterization of Shiga toxin-producing Escherichia coli O157:H7 and non-O157 from beef carcasses at a slaughter plant in Mexico

Author:
Varela-Hernandez, J.J.; Cabrera-Diaz, E.; Cardona-Lopez, M.A.; Ibarra-Velazquez, L.M.; Rangel-Villalobos, H.; Castillo, A.; Torres-Vitela, M.R.; Ramirez-Alvarez, A.
Source:
International journal of food microbiology 2007 v.113 no.2 pp. 237-241
ISSN:
0168-1605
Subject:
slaughter; Shiga-like toxins; slaughterhouses; beef carcasses; bacterial contamination; meat processing; food contamination; immunomagnetic separation; beef cattle; HACCP; food pathogens; genes; beef; Escherichia coli O157; virulence; Mexico
Abstract:
... The contamination of beef carcasses with Shiga toxin-producing O157:H7 and non-O157 Escherichia coli (STEC) obtained from a slaughter plant in Guadalajara, Mexico was investigated. A total of 258 beef carcasses were sampled during a 12-month period. All samples were assayed for STEC by selective enrichment in modified tryptone soy broth supplemented with cefixime, cefsulodin and vancomycin, follow ...
DOI:
10.1016/j.ijfoodmicro.2006.06.028

37. Occurrence of Shiga toxin-producing Escherichia coli in a Spanish raw ewe's milk cheese

Author:
Caro, I.; García-Armesto, M.R.
Source:
International journal of food microbiology 2007 v.116 no.3 pp. 410-413
ISSN:
0168-1605
Subject:
cheese milk; Shiga-like toxins; bacterial contamination; raw milk; semisoft cheeses; hard cheeses; cheese ripening; Escherichia coli O157; polymerase chain reaction; food pathogens; genes; genotype; ewe milk; virulence
Abstract:
... The aim of the present study was to investigate the occurrence of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli (STEC) in 'Castellano' cheese, a non-cooked and hard or semi-hard Spanish cheese made from ewe's milk. A total of 83 raw milk cheese samples with different ripening times (2.5, 6 and 12 months) were taken at 30 cheese factories. Samples were examined for the presence o ...
DOI:
10.1016/j.ijfoodmicro.2007.02.015

38. Phage types, virulence genes and PFGE profiles of Shiga toxin-producing Escherichia coli O157:H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru)

Author:
Mora, A.; Leon, S.L.; Blanco, M.; Blanco, J.E.; Lopez, C.; Dahbi, G.; Echeita, A.; Gonzalez, E.A.; Blanco, J.
Source:
International journal of food microbiology 2007 v.114 no.2 pp. 204-210
ISSN:
0168-1605
Subject:
Shiga-like toxins; bacterial contamination; raw vegetables; ground beef; food contamination; bacteriophages; soft cheeses; food pathogens; genes; Escherichia coli O157; raw meat; virulence; Peru
Abstract:
... The present study was conducted in Lima Metropolitana to evaluate the prevalence of Shiga toxin-producing Escherichia coli (STEC) O157:H7 in raw beef, raw ground beef, soft cheese and fresh vegetables, sampled at different markets in the city. Between October 2000 and February 2001, 407 food samples were collected from different markets in the 42 districts of Lima Metropolitana. Samples were assay ...
DOI:
10.1016/j.ijfoodmicro.2006.09.009

39. Physiological Response and Protein Expression under Acid Stress of Escherichia coli O157:H7 TWC01 Isolated from Taiwan

Author:
Huang, Y.J.; Tsai, T.Y.; Pan, T.M.
Source:
Journal of agricultural and food chemistry 2007 v.55 no.17 pp. 7182-7191
ISSN:
0021-8561
Subject:
acid tolerance; Shiga-like toxins; proteome; protein synthesis; microbial physiology; bacterial proteins; Escherichia coli O157; protein secretion; strains; Escherichia infections; protein composition; Taiwan
Abstract:
... Escherichia coli O157:H7 has an unusually high resistance to acidic environments. Some research has revealed that acid-adapted cells, by exposure to moderately acidic conditions, are more resistant to a subsequent strong acidic challenge or other stress. This study was conducted to understand the protein expression regulation of acid tolerance response (ATR) of a local isolated E. coli O157:H7 TWC ...
DOI:
10.1021/jf071014s

40. Presence of Shiga toxin-producing E. coli O157:H7 in a survey of wild artiodactyls

Author:
Garcia-Sanchez, A.; Sanchez, S.; Rubio, R.; Pereira, G.; Alonso, J.M.; Hermoso de Mendoza, J.; Rey, J.
Source:
Veterinary microbiology 2007 v.121 no.3-4 pp. 373-377
ISSN:
0378-1135
Subject:
Shiga-like toxins; Artiodactyla; mouflon; hemolysins; wild animals; immunologic techniques; screening; epidemiological studies; humans; polymerase chain reaction; pathogen identification; Escherichia infections; disease reservoirs; zoonoses; disease transmission; viral antigens; Escherichia coli O157; deer; intimin; Spain
Abstract:
... This study was carried out to evaluate the role of wild artiodactyls as reservoirs of Escherichia coli O157:H7 for livestock and humans. Retroanal mucosal swabs samples from 206 red deer (Cervus elaphus), 20 roe deer (Capreolus capreolus), 6 fallow deer (Dama dama) and 11 mouflon (Ovis musimon), collected during the hunting season (autumn-winter) in South-western Spain, were screened. Samples were ...
DOI:
10.1016/j.vetmic.2006.12.012

41. Screening food raw materials for the presence of the world's most frequent clinical cases of Shiga toxin-encoding Escherichia coli O26, O103, O111, O145 and O157

Author:
Perelle, S.; Dilasser, F.; Grout, J.; Fach, P.
Source:
International journal of food microbiology 2007 v.113 no.3 pp. 284-288
ISSN:
0168-1605
Subject:
epidemiological studies; Shiga-like toxins; minced meat; raw milk; serotypes; polymerase chain reaction; rapid methods; enzyme-linked immunosorbent assay; food pathogens; beef; microbial detection; Escherichia coli O157; screening; virulence; France
Abstract:
... This work aims to provide a strategy for rapidly screening food raw materials of bovine origin for the presence of the most frequent O-serogroups of Shiga toxin-encoding Escherichia coli (STEC) involved in food poisoning outbreaks. The prevalence of highly pathogenic serogroups of STEC was surveyed in 25 g portions of minced meat and raw milk using PCR-ELISA and multiplex real-time PCR assays. The ...
DOI:
10.1016/j.ijfoodmicro.2006.08.014

42. Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in São Paulo, Brazil

Author:
Aidar-Ugrinovich, L.; Blanco, J.; Blanco, M.; Blanco, J.E.; Leomil, L.; Dahbi, G.; Mora, A.; Onuma, D.L.; Silveira, W.D.; Castro, A.F.P. de
Source:
International journal of food microbiology 2007 v.115 no.3 pp. 297-306
ISSN:
0168-1605
Subject:
Shiga-like toxins; bacterial infections; bacterial contamination; serotypes; food contamination; enteropathogenic Escherichia coli; intimin; animal pathogenic bacteria; food pathogens; genes; microbial detection; prevalence; veal calves; virulence; on-farm food safety; Brazil
Abstract:
... Shiga toxin-producing Escherichia coli (STEC), is the most important recently emerged group of foodborne pathogens. Ruminants, especially cattle, have been implicated as a principal reservoir of STEC, undercooked ground beef and raw milk being the major vehicles of foodborne outbreaks. Enteropathogenic E. coli (EPEC) strains are defined as eae-harboring diarrheagenic E. coli that possess the abili ...
DOI:
10.1016/j.ijfoodmicro.2006.10.046

43. Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia

Author:
Vu-Khac, H.; Holoda, E.; Pilipcinec, E.; Blanco, M.; Blanco, J.E.; Dahbi, G.; Mora, A.; López, C.; González, E.A.; Blanco, J.
Source:
Veterinary journal 2007 v.174 no.1 pp. 176-187
ISSN:
1090-0233
Subject:
Shiga-like toxins; phylogeny; enteropathogenic Escherichia coli; pulsed-field gel electrophoresis; genes; diarrhea; seroprevalence; swine diseases; pathotypes; virulence; epidemiological studies; enterotoxins; fimbriae; polymerase chain reaction; Escherichia infections; microbial genetics; disease prevalence; piglets; intimin; Slovakia
Abstract:
... Two hundred and fifty Escherichia coli isolates from diarrhoeic and healthy piglets were serotyped and tested for the presence of virulence genes for fimbriae, intimin, heat-labile (LT) and heat-stable (STa and STb) enterotoxins, Stx toxins, and enteroaggregative heat-stable 1 (EAST1) enterotoxin by polymerase chain reaction (PCR). Although 220 isolates from diarrhoeic piglets belonged to 43 O ser ...
DOI:
10.1016/j.tvjl.2006.05.019
PubMed:
16956777

44. The effect of calcium ions on adhesion and competitive exclusion of Lactobacillus ssp. and E. coli O138

Author:
Larsen, N.; Nissen, P.; Willats, W.G.T.
Source:
International journal of food microbiology 2007 v.114 no.1 pp. 113-119
ISSN:
0168-1605
Subject:
in vitro studies; culture media; calcium; ions; probiotics; lactic acid bacteria; Lactobacillus rhamnosus; Lactobacillus reuteri; Lactobacillus jensenii; radiolabeling; bacterial adhesion; enteropathogenic Escherichia coli; food pathogens; bacterial contamination; food contamination; competitive exclusion; food preservation; Shiga-like toxins
Abstract:
... The adhesion abilities of 11 strains of Lactobacillus were determined in vitro using the IPEC-J2 cell line as a model system. Bacteria cultures included the probiotic strains L. rhamnosus GG, L. reuteri ATCC 55730, L. johnsonii NCC 533 and L. reuteri DSM 12246, and new isolates of Lactobacillus ssp. Adhesion was quantified by scintillation counting of radiolabelled bound bacteria. The highest adhe ...
DOI:
10.1016/j.ijfoodmicro.2006.10.033

45. Antimicrobial resistance in Irish isolates of verocytotoxigenic Escherichia coli (E. coli)--VTEC

Author:
Walsh, C.; Duffy, G.; O'Mahony, R.; Fanning, S.; Blair, I.S.; McDowell, D.A.
Source:
International journal of food microbiology 2006 v.109 no.3 pp. 173-178
ISSN:
0168-1605
Subject:
Shiga-like toxins; gene transfer; minced meat; bacterial contamination; humans; hides and skins; antibiotic resistance; antibiotics; food contamination; multiple drug resistance; biomarkers; serotypes; veterinary medicine; food pathogens; beef; genes; genomics; Escherichia coli O157; virulence; Ireland
Abstract:
... This study compared the antimicrobial resistance profiles of Escherichia coli O157:H7 isolates (n = 257) recovered from bovine hides, minced beef and human clinical samples in Ireland, to those profiles of a range of Irish non-O157 E. coli (O111 and O26) isolates (n = 31) from a variety of clinical and veterinary sources. Four multi-drug resistant (MDR) E. coli O157:H7 food isolates were identifie ...
DOI:
10.1016/j.ijfoodmicro.2006.01.023

46. Characteristics of cytotoxic necrotizing factor and cytolethal distending toxin producing Escherichia coli strains isolated from meat samples in Northern Ireland

Author:
Kadhum, H.J.; Ball, H.J.; Oswald, E.; Rowe, M.T.
Source:
Food microbiology 2006 v.23 no.5 pp. 491-497
ISSN:
0740-0020
Subject:
cytotoxins; pork; Shiga-like toxins; beef carcasses; minced meat; lamb meat; bacterial contamination; serotypes; food contamination; polymerase chain reaction; pig carcasses; ovine carcasses; beef; microbial detection; food pathogens; genes; virulence; Escherichia coli; Ireland
Abstract:
... Swabs collected from pig, lamb and beef carcasses and samples of pork, lamb and beef mince were cultured for Escherichia coli strains. Strains harbouring cytotoxic necrotizing factors (CNF1 and 2) and cytolethal distending toxins (CDT-I,-II,-III and -IV) were identified in plate cultures of the isolates by colony hybridization with labelled probes and multiplex PCR assays. Simplex and multiplex PC ...
DOI:
10.1016/j.fm.2005.07.003
PubMed:
16943042

47. Characterization of Escherichia coli isolated from carcasses of beef cattle during their processing at an abattoir in Brazil

Author:
Rigobelo, E.C.; Stella, A.E.; Avila, F.A.; Macedo, C.; Marin, J.M.
Source:
International journal of food microbiology 2006 v.110 no.2 pp. 194-198
ISSN:
0168-1605
Subject:
Shiga-like toxins; slaughterhouses; beef carcasses; bacterial contamination; meat processing; antibiotic resistance; serotypes; multiple drug resistance; food pathogens; genes; virulence; Escherichia coli
Abstract:
... Beef carcass sponge samples collected between March 2003 and August 2005 at an abattoir in Brazil were surveyed for the presence of Shiga toxin-producing Escherichia coli (STEC). Only one carcass among the 80 tested showed a STEC, stx2-encoding gene by PCR amplification. The frequency of carcass contamination by E. coli during processing was tested at three situations, respectively: preevisceratio ...
DOI:
10.1016/j.ijfoodmicro.2006.03.013

48. Comparison of E. coli O157 and Shiga toxin-encoding genes (stx) prevalence between Ohio, USA and Norwegian dairy cattle

Author:
LeJeune, J.T.; Hancock, D.; Wasteson, Y.; Skjerve, E.; Urdahl, A.M.
Source:
International journal of food microbiology 2006 v.109 no.1-2 pp. 19-24
ISSN:
0168-1605
Subject:
dairy cows; dairy herds; Shiga-like toxins; bacterial contamination; toxigenic strains; serotypes; food pathogens; feed contamination; dairy farming; genes; prevalence; Escherichia coli O157; virulence; on-farm food safety
Abstract:
... Environmental and food contamination with Shiga toxin-producing Escherichia coli (STEC) pose a threat to public health worldwide, with notable geographic differences in incidence of human disease caused by these organisms. The prevalence of E. coli O157 and total stx-positive specimens collected from mature dairy cattle in Ohio and Norwegian dairy farms was compared using identical laboratory meth ...
DOI:
10.1016/j.ijfoodmicro.2006.01.005

49. Detection and frequency of VT1, VT2 and eaeA genes in Escherichia coli O157 and O157:H7 strains isolated from cattle, cattle carcasses and abattoir environment in Istanbul

Author:
Yilmaz, A.; Gun, H.; Ugur, M.; Turan, N.; Yilmaz, H.
Source:
International journal of food microbiology 2006 v.106 no.2 pp. 213-217
ISSN:
0168-1605
Subject:
Shiga-like toxins; slaughterhouses; beef carcasses; bacterial contamination; food contamination; polymerase chain reaction; beef cattle; feces; food pathogens; genes; gene frequency; Escherichia coli O157; detection; virulence; intimin; Turkey (country)
Abstract:
... The aim of this study was to detect VT1, VT2 and eaeA genes and to determine the frequency of these genes in Escherichia coli O157 and O157:H7 strains isolated from cattle, cattle carcasses and environmental samples of the 5 abattoirs located in Istanbul, Turkey. For this, the presence of VT1, VT2 and eaeA genes in 26 strains of E. coli O157:H7 and 6 strains of O157 was investigated by multiplex-P ...
DOI:
10.1016/j.ijfoodmicro.2005.05.018

50. Effect of trisodium phosphate adaptation on changes in membrane lipid composition, verotoxin secretion, and acid resistance of Escherichia coli O157:H7 in simulated gastric fluid

Author:
Yuk, H.G.; Marshall, D.L.
Source:
International journal of food microbiology 2006 v.106 no.1 pp. 39-44
ISSN:
0168-1605
Subject:
acid tolerance; Shiga-like toxins; bacterial contamination; sodium phosphate; membrane fluidity; avirulent strains; food contamination; virulence; gastric acid; food pathogens; salt concentration; pH; mutants; genes; Escherichia coli O157; lipid bilayers; culture media; food preservatives
Abstract:
... Escherichia coli O157:H7 (HEC), E. coli O157:H7 rpoS mutant (HEC-RM), and nonpathogenic E. coli (NPEC) were step-wise adapted to trisodium phosphate (TSP) by incubation in broths of increasing concentration, from 0% to 0.6%, at 37 °C for 24 h. After incubation at each concentration, each population was examined for acid resistance (D value) in simulated gastric fluid of pH 1.5, cell envelope membr ...
DOI:
10.1016/j.ijfoodmicro.2005.05.009

51. Immunoconcentration of Shiga toxin-producing Escherichia coli O157 from animal faeces and raw meats by using Dynabeads anti-E. coli O157 and the VIDAS system

Author:
Islam, M.A.; Heuvelink, A.E.; Talukder, K.A.; Boer, E. de
Source:
International journal of food microbiology 2006 v.109 no.1-2 pp. 151-156
ISSN:
0168-1605
Subject:
disease reservoirs; Shiga-like toxins; bacterial contamination; antibody detection; food contamination; feces; rapid methods; food pathogens; genes; microbial detection; farmed animal species; Escherichia coli O157; raw meat; virulence; on-farm food safety
Abstract:
... To identify the reservoirs and routes of transmission of Shiga toxin-producing Escherichia coli (STEC) O157, sensitive detection and isolation methods are necessary. The sensitivity of traditional culture methods can be improved significantly by the inclusion of an immunoconcentration step, resulting in less false-negatives. In this report, we evaluated the results of two commercially available te ...
DOI:
10.1016/j.ijfoodmicro.2006.01.010

52. Isolation and characterization of Shiga toxin-producing Escherichia coli from precooked sausages

Author:
Oteiza, J.M.; Chinen, I.; Miliwebsky, E.; Rivas, M.
Source:
Food microbiology 2006 v.23 no.3 pp. 283-288
ISSN:
0740-0020
Subject:
food spoilage; Shiga-like toxins; Staphylococcus aureus; genes; traditional foods; food sanitation; precooked foods; bacterial contamination; feces; virulence; aerobes; Escherichia coli; coliform bacteria; yeasts; plate count; microbial detection; molds (fungi); food contamination; food pathogens; sausages; Argentina
Abstract:
... The aim of the study was to establish the microbiological quality of morcillas, typical Argentine sausages, and to investigate the presence of Shiga toxin-producing Escherichia coli (STEC). Between October 2001 and October 2002, a total of 100 morcilla samples were analysed. Several samples showed high levels of total aerobic mesophilic bacteria counts, molds and yeasts. The samples analysed conta ...
DOI:
10.1016/j.fm.2005.04.003

53. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 from Turkish cattle

Author:
Aslantas, O.; Erdogan, S.; Cantekin, Z.; Gulactı, I.; Evrendilek, G.A.
Source:
International journal of food microbiology 2006 v.106 no.3 pp. 338-342
ISSN:
0168-1605
Subject:
slaughter; Shiga-like toxins; slaughterhouses; bacterial contamination; cytotoxicity; food contamination; selective media; beef cattle; immunomagnetic separation; polymerase chain reaction; rectum; food pathogens; enrichment culture; genes; Escherichia coli O157; virulence; Turkey (country)
Abstract:
... The objective of this study was to collect rectal swabs from the cattle in a slaughterhouse located in Hatay (Turkey) immediately after slaughter for the isolation and characterization of verotoxin-producing Escherichia coli O157 in each month during a 1-year period. The rectal swab samples were analyzed for the isolation of E. coli O157 through preenrichment, immunomagnetic separation and selecti ...
DOI:
10.1016/j.ijfoodmicro.2005.08.005

54. Occurrence of non-O157 shiga toxin-producing Escherichia coli in ready-to-eat food from supermarkets in Argentina

Author:
Balague, C.; Khan, A.A.; Fernandez, L.; Redolfi, A.L.; Aquili, V.; Voltattorni, P.; Hofer, C.; Ebner, G.; Duenas, S.; Cerniglia, C.E.
Source:
Food microbiology 2006 v.23 no.3 pp. 307-313
ISSN:
0740-0020
Subject:
supermarkets; Shiga-like toxins; bacterial contamination; ready-to-eat foods; food contamination; polymerase chain reaction; food pathogens; pulsed-field gel electrophoresis; microbial detection; genes; virulence; Escherichia coli; Argentina
Abstract:
... Between June 2000 and December 2001, 500 food samples were collected from supermarkets and shops selling ready-to-eat food in Rosario, Argentina, and examined for Escherichia coli. Forty-nine E. coli isolates from food samples were further characterized for virulence genes by multiplex polymerase chain reaction (PCR) targeting the stx1, stx2, stx2e, eaeA, CNF1, CNF2, Einv, LTI, STI, and STII genes ...
DOI:
10.1016/j.fm.2005.03.005
PubMed:
16943019

55. Prevalence, serotypes and virulence genes of Shiga toxin-producing Escherichia coli isolated from ovine and caprine milk and other dairy products in Spain

Author:
Rey, J.; Sanchez, S.; Blanco, J.E.; Hermoso de Mendoza, J.; Hermoso de Mendoza, M.; Garcia, A.; Gil, C.; Tejero, N.; Rubio, R.; Alonso, J.M.
Source:
International journal of food microbiology 2006 v.107 no.2 pp. 212-217
ISSN:
0168-1605
Subject:
disease reservoirs; fresh cheeses; Shiga-like toxins; bacterial contamination; milk curds; taxonomy; serotypes; food contamination; milk tanks; food pathogens; genes; genotype; ewe milk; goat milk; virulence; Escherichia coli; Spain
Abstract:
... The aim of this study was to determinate the prevalence, serotypes and virulence genes of Shiga toxin-producing Escherichia coli (STEC) strains isolated from different dairy products (DP) in Spain with the purpose of determining whether DP represent a potential source of STEC pathogenic for humans. A total of 502 DP were examined from 64 different ovine and caprine flocks and 6 dairy plants in Ext ...
DOI:
10.1016/j.ijfoodmicro.2005.08.025

56. Shiga toxin production by sausage-borne Escherichia coli O157:H7 in response to a post-processing in vitro digestion challenge

Author:
Naim, F.; Leblanc, D.; Messier, S.; Saucier, L.; Piette, G.; Houde, A.
Source:
Food microbiology 2006 v.23 no.3 pp. 231-240
ISSN:
0740-0020
Subject:
dried meat; Shiga-like toxins; fermentation; bacterial contamination; in vitro digestion; fermented foods; cytotoxicity; food contamination; food pathogens; hemolytic uremic syndrome; sausages; Escherichia coli O157; drying; virulence
Abstract:
... In order to study the effects of the fermentation-drying procedure and subsequent in vitro digestion on Shiga toxins (Stx) production by Escherichia coli O157:H7, dry sausages were inoculated during the formulation step with pure cultures of strains 5-1 and ATCC 43895. The inoculated sausages were submitted to a minimum (30 min, pH between 3.1 and 3.5) or a maximum (120 min at stepwise adjusting t ...
DOI:
10.1016/j.fm.2005.04.006
PubMed:
16943009

57. Shiga toxin-producing Escherichia coli in ground beef and lamb cuts: Results of a one-year study

Author:
Barlow, R.S.; Gobius, K.S.; Desmarchelier, P.M.
Source:
International journal of food microbiology 2006 v.111 no.1 pp. 1-5
ISSN:
0168-1605
Subject:
Shiga-like toxins; slaughterhouses; bacterial contamination; lamb meat; serotypes; ground beef; food contamination; polymerase chain reaction; food pathogens; genes; microbial detection; prevalence; Escherichia coli O157; meat cuts; virulence; traceability
Abstract:
... Shiga toxin-producing Escherichia coli (STEC) have been associated with a broad spectrum of diarrhoeal syndromes. Some of these cases have been attributed to foods of bovine origin or other foods cross-contaminated by beef products or cow manure. The purpose of this study was to determine the pattern of STEC distribution in selected red meats over time. Samples of ground beef and lamb cuts were co ...
DOI:
10.1016/j.ijfoodmicro.2006.04.039

58. Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction

Author:
Perelle, S.; Dilasser, F.; Grout, J.; Fach, P.
Source:
Journal of applied microbiology 2005 v.98 no.5 pp. 1162-1168
ISSN:
1364-5072
Subject:
food pathogens; enteropathogens; enteropathogenic Escherichia coli; pathogen identification; Shiga-like toxins; bacterial antigens; biosynthesis; multigene family; polymerase chain reaction; human health
Abstract:
... Aims: The aims of the study were to identify the specific genes of O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O103 and to provide the basis for a specific real-time PCR test for rapid detection of E. coli O103. Methods and Results: The published primers complementary to JUMPstart and gnd gene, the conserved flanking sequences of O-antigen genes clusters in E. coli an ...
DOI:
10.1111/j.1365-2672.2005.02545.x
PubMed:
15836486

59. Prevalence of Shiga toxin-producing Escherichia coli in beef

Author:
Hussein, H.S.; Bollinger, L.M.
Source:
Meat science 2005 v.71 no.4 pp. 676-689
ISSN:
0309-1740
Subject:
beef; microbial detection; Escherichia coli; Shiga-like toxins; beef cattle; food contamination; bacterial contamination; food pathogens; serotypes
Abstract:
... Over the past two decades, many human illness outbreaks were attributed to consumption of undercooked beef products containing Shiga toxin-producing Escherichia coli (STEC). The illnesses included mild or bloody diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic syndrome (HUS). Tracing these outbreaks to O157 and an increasing number of non-O157 STEC strains suggests that bee ...
DOI:
10.1016/j.meatsci.2005.05.012
PubMed:
22061213

60. Use of comparative genomics as a tool to assess the clinical and public health significance of emerging Shiga toxin-producing Escherichia coli serotypes

Author:
Karmali, M.A.
Source:
Meat science 2005 v.71 no.1 pp. 62-71
ISSN:
0309-1740
Subject:
animal pathology; Shiga-like toxins; public health; microbial genetics; serotypes; pathogenesis; pandemic; genes; genomics; food pathogens; Escherichia infections; Escherichia coli O157; foodborne illness; virulence
Abstract:
... Shiga toxin (Stx)-producing Escherichia coli (STEC) cause sporadic or epidemic food- or water-borne illness whose clinical spectrum includes diarrhea, hemorrhagic colitis, and the potentially fatal hemolytic uremic syndrome (HUS). Over 200 STEC serotypes have now been implicated in human disease. Serotype O157:H7 is associated most outbreaks and most cases of HUS. Other serotypes are also associat ...
DOI:
10.1016/j.meatsci.2005.03.001
PubMed:
22064052

61. Implication of Virulence Factors in Escherichia coli O157:H7 Pathogenesis

Author:
Jason J. LeBlanc
Source:
Critical reviews in microbiology 2003 v.29 no.4 pp. 277-296
ISSN:
1549-7828
Subject:
Escherichia coli O157; Shiga-like toxins; colitis; gastrointestinal diseases; microvilli; pathogenesis; phenotype; public health; virulence
Abstract:
... Since the first documented outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 in 1982, numerous publications have demonstrated or proposed putative components implicated in the pathogenesis of this gastrointestinal infection. Indeed, Escherichia coli O157:H7 pathogenesis is linked to several potential virulent factors such as verotoxins (or Shiga-like toxins), components implicated ...
DOI:
10.1080/713608014

62. Quantitative detection of Escherichia coli O157:H7 in ground beef by immunomagnetic separation and competitive polymerase chain reaction

Author:
Guan, J.; Levin, R.E.
Source:
Food biotechnology 2002 v.16 no.3 pp. 155-166
ISSN:
0890-5436
Subject:
nucleic acid hybridization; Shiga-like toxins; Escherichia coli; quantitative analysis; ground beef; food contamination; microbial contamination; polymerase chain reaction; food microbiology; magnetic separation; nucleotide sequences; immunologic techniques; detection
Abstract:
... A protocol based on immunomagnetic separation (IMS) and competitive polymerase chain reaction (C-PCR) was developed for quantitative detection of Escherichia coli O157:H7 in ground beef. Internal standard sequences were synthesized by PCR with hybrid primers composed in part of original primers for C-PCR. A single concentration of internal standards was used for C-PCR after calibration with known ...
DOI:
10.1081/FBT-120016665

63. Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR

Author:
Hu, Y.; Zhang, Q.; Meitzler, J.C.
Source:
Journal of applied microbiology 1999 v.87 no.6 pp. 867-876
ISSN:
1364-5072
Subject:
Escherichia coli O157; Shiga-like toxins; adhesins; animal pathogens; animal production; bacteria; cattle; colitis; feces; food animals; genes; humans; intimin; polymerase chain reaction; serotypes; virulence
Abstract:
... Cattle are considered the major reservoir for Escherichia coli O157:H7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. A sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically needed to monitor the emergence, transmission, and colonization of this pathogen ...
DOI:
10.1046/j.1365-2672.1999.00938.x
PubMed:
10664910