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... Acidogenic fermentation (FM treatment) converts organics in waste sludge to valuable short-chain fatty acids (SCFAs). To maintain a favorable condition for the production of SCFAs, an alkali is often added continuously to maintain an alkaline pH in the fermenter. However, this chemical adjustment is costly and biotic hydrolysis is slow. In this research, thermal hydrolysis (TH) was introduced as a ...
... In this work, an innovative technology by using ferrous sulfate combined with digestate, was applied to the Cr (VI) reduction. In the combined process, 3% ferrous sulfate, 5% digestate, 2% glucose, 30 °C and 50% moisture content were proved to be the optimal operating conditions. The combined process achieved 100% reduction of 3000 mg/Kg Cr (VI) within 10 days. Ferrous sulfate and digestate had a ...
... Here, a novel combined heterotrophic and bio-electrochemical hydrogen autotrophic (CHBHA) system was developed to remove perchlorate under low chemical dosages and energy consumption. The perchlorate removal performance at various hydraulic retention times (HRTs) and acetate dosages was investigated. For influent containing 10 ± 0.10 mg/L perchlorate, the optimal removal efficiency by the CHBHA sy ...
... Antibiotics in waste activated sludge (WAS) has drawn increasing attention because of their persistent and bioaccumulation characteristics. Most study illustrated the role of antibiotics in anaerobic fermentation from WAS, but lacking the analysis at microbial level as well as the possible interaction between them. This study investigated the effect of three sulfonamide antibiotics (sulfamethoxazo ...
... Anaerobic digestion (AD) experiments were conducted on an industrial algae strain of Nannochloropsis salina in two identical 5-liter digesters for about 300 days. One digester was fed with whole cell algal biomass (WCB) while another with lipid-extracted algal biomass residues (LEB). WCB digester shows higher specific methane productivity ranging from 0.59 to 0.65 m³ CH₄/kgVS, while that in LEB di ...
... The sulfate reduction behavior of the landfill leachate saturated zone under different temperatures was investigated. The results showed that temperature had significant effects on sulfate reduction behavior. The sulfate reduction efficiency was the highest at high temperatures (55 °C and 45 °C), followed by mesophilic temperature (35 °C). Normal temperature 25 °C was far less effective than 55 °C ...
... In this work, the impact of initial pH on the production of volatile fatty acids (VFAs) of hybrid Pennisetum was investigated. The batch experiments were conducted under six distinct beginning pH at a mesophilic temperature. Initial pH had an obvious effect on VFA yield and composition, and severe alkaline circumstances (pHᵢₙ = 11.0) could boost VFA production and acetic acid selectivity. The high ...
... BACKGROUND: Lignin intermediates resulting from lignocellulose degradation have been suspected to hinder anaerobic mineralisation of organic materials to biogas. Phenyl acids like phenylacetate (PAA) are early detectable intermediates during anaerobic digestion (AD) of aromatic compounds. Studying the phenyl acid formation dynamics and concomitant microbial community shifts can help to understand ...
... Despite the significant influence of the microbial communities that inhabit the ruminant’s gastrointestinal tract on their health and production, the study of the composition and variations in sheep and goats gut microbiome is limited. The present study investigated the faecal microbiomes of sheep and goats at 6 and 12 months of age using Illumina MiSeq, targeting the 16S rRNA gene V3-V4 region se ...
Aequorivita; Brachymonas; Comamonas; Tissierella; air flow; ammonia; bacteria; bioreactors; chemical oxygen demand; community structure; microbial communities; nitrogen; pH; prokaryotic cells; redox potential; sludge; total nitrogen; total phosphorus; wastewater; wastewater treatment
Abstract:
... In this study, a sequencing batch and intermittent air-lift bioreactor (SBIAB) was evaluated under the three independent variables to treat raw piggery wastewater. The effects of hydraulic retention time (HRT), air flow rate and sludge retention time (SRT) on the nutrients removal of SBIAB were researched. The optimum values of HRT, air flow rate and SRT were 8 d, 2 l/min and 20 d, respectively. M ...
Bacteroides; Clostridium; Rhodocyclaceae; Sphingobacteriia; Tissierella; activated sludge; bacterial communities; chemical oxygen demand; community structure; farms; gamma-Proteobacteria; public health; swine; total nitrogen; total phosphorus; wastewater; wastewater treatment
Abstract:
... Chemicals of emerging concern (CEC) in pig farm breeding wastewater, such as antibiotics, will soon pose a serious threat to public health. It is therefore essential to consider improving the treatment efficiency of piggery wastewater in terms of microorganisms. In order to optimize the overall piggery wastewater treatment system from the perspective of the bacterial community structure and its re ...
Lysinibacillus; Shewanella; Tissierella; biodegradation; electron transfer; mineralization; photolysis; pollutants; pyridines; research; total organic carbon; toxicity; wastewater treatment; water
Abstract:
... Pyridine, a highly toxic nitrogen-containing heterocyclic compound, is recalcitrant in the conventional biodegradation process. In this study, BiVO₄/FeOOH semiconductor-microbe interface was developed for enhanced visible-light-driven biodegradation of pyridine, where the efficiencies of pyridine removal (100%), total organic carbon (TOC) removal (88.06±3.76%) and NH₄⁺-N formation (84.51±8.95%) we ...
... The Microbial fuel cell (MFC) technology harnesses the potential of some naturally occurring bacteria for electricity generation. Digested sludge is commonly used as the inoculum to initiate the process. There are, however, health hazards and practical issues associated with the use of digested sludge depending on its origin as well as the location for system deployment. This work reports the deve ...
... Tributyrin and essential oils have been used as alternatives to antimicrobials to improve gut health and growth performance in piglets. This study was to evaluate the effects of a dietary supplement with two encapsulated products containing different combinations of tributyrin with oregano or with methyl salicylate on growth performance, serum biochemical parameters related to the physiological st ...
... Lignite could be used to promote methane production during high-solid anaerobic co-digestion (HS-AcoD) of pig manure, however, the effects of lignite amendment on the fate of ARGs during HS-AcoD are unknown. Here, we explored the influence of lignite (0%, 8%, 16%, 32%, and 64%) on the fate of ARGs during HS-AcoD of pig manure. The results showed that 16% lignite reduced the absolute abundance of A ...
... Alkaline fermentation has been considered as one of the efficient methods for waste activated sludge (WAS) treatment, but usually limited by microbial fermentation activities under extreme pH condition. One newly isolated alkali-tolerant strain Corynebacterium pollutisoli SPH6 was used to assess its potential role and effect on WAS alkaline fermentation process. Results from response surface metho ...
Arcobacter; Methanoculleus; Ruminococcaceae; Tissierella; acclimation; biotechnology; fermentation; gas production (biological); hypersalinity; methane; methane production; methanogens; microbial communities; molasses; salt stress; salt tolerance; total organic carbon; wastewater
Abstract:
... This study evaluated the performance of an upflow anaerobic filter (UAF) reactor in the thermophilic methane fermentation of hypersaline molasses wastewater. The high salinity (~ 45 mS/cm) of the undiluted wastewater completely inhibited the biogas production. An acclimation strategy involving gradient dilution of the molasses wastewater was implemented to gradually increase the salt stress. Conse ...
Tissierella; amino acid metabolism; anaerobic digesters; anaerobic digestion; bacteria; glutamic acid; hot water treatment; hydrolysis; proline; sewage sludge; waste management
Abstract:
... Three semi-continuous anaerobic digesters with raw sludge (R1) or thermally hydrolyzed sludge (120 °C (R2) or 160 °C (R3)) were operated to investigate the effects of thermal hydrolysis on the metabolism of amino acids during high solid anaerobic digestion with sewage sludge. Thermal hydrolysis pretreatment (THP) decomposed protein in terms of hydrolytic amino acid in raw sludge by 8.90% and 26.69 ...
Polina A. Bukhtiyarova; Dmitry V. Antsiferov; Gael Brasseur; Marat R. Avakyan; Yulia A. Frank; Olga P. Ikkert; Nikolay V. Pimenov; Olli H. Tuovinen; Olga V. Karnachuk
... Recent reports on antimicrobial effects of metallic Cu prompted this study of anaerobic microbial communities on copper surfaces. Widely circulating copper-containing coinage was used as a potential source for microorganisms that had had human contact and were tolerant to copper. This study reports on the isolation, characterization, and genome of an anaerobic sulfidogenic Tissierella sp. P1from c ...
... The present work studied to evaluate the effectiveness under the interaction of bacterial consortium and biochar applied to give impetus to bacterial community activities among cow manure composting. High-throughput sequencing technique and six treatments were carried out: T2, T3 and T6 were single apply of bacteria culture (C), 12%wood biochar (12%WB) and 12%wheat straw biochar (12%WSB), respecti ...
... p-Cresol (PC) is a potential off-flavor and carcinogenic compound that affects food flavor and safety. However, controlling the production of PC when making fermented food is hindered by a lack of knowledge of the microbial diversity and the growth requirements of the microbiota that produce PC. To address this, the present study used three media with selected carbon sources (glucose, ethanol and ...
... A three-stage anaerobic digester setup was configured and evaluated for enhanced methane production during co-digestion of food waste and waste activated sludge and the corresponding bacterial and methanogen communities were characterized. Results showed that the average methane yield (0.496 L/gVS) in the three-stage digester was 13–52% higher than that of one- and two-stage digesters. Compared to ...
... Different mesophilic and thermophilic methanogenic consortia were acclimatised and enriched to extreme total ammonia (9.0 and 5.0 g NH₄⁺-N L⁻¹, respectively) and free ammonia (1.0 and 1.4 g NH₃-N L⁻¹, respectively) levels in this study. [2-¹⁴C] acetate radioisotopic analyses showed the dominance of aceticlastic methanogenesis in all enriched consortia. According to 16S rRNA gene sequencing result, ...
... Anaerobic microbiological antimonate [Sb(V)] respiration is a newly discovered process regulating the Sb redox transformation in soils. However, little is known about the role microbiological Sb(V) respiration plays in the fate of Sb in the subsurface, especially in the presence of sulfate and electron shuttles. Herein, we successfully enriched a Sb(V) reducing microbiota (SbRM) from the subsurfac ...
... This study revealed for the first time the dynamic changes of the bacterial community during the fermentation of traditional Chinese fish sauce (TCFS) using high-throughput sequencing. In the early phase of TCFS fermentation, Shewanella (approximately 90%) within Proteobacteria was the dominant bacteria. Then, Halanaerobium (3%–86%) within Firmicutes rapidly replaced Shewanella as the domina ...
... This study focused on investigating reactor performance, simultaneous methanogeneis and denitrifiction (SMD) process for treatment of a sulfate plus organic sulfur – rich 3,4,5-Triethoxybenzaldehyde (TMBA) manufacturing wastewater with variable COD/TSO42− (total sulfate) ratio by micro-electric field- zero-valent-iron (ZVI) UASB for 390 days. The initial COD/TSO42− was set as 1.42, 0.9 and 0.5, re ...
... The bacterial diversity in fermenting dye vats with woad (Isatis tinctoria L.) prepared and maintained in a functional state for approximately 12 months was examined using a combination of culture-dependent and -independent PCR-DGGE analyses and next-generation sequencing of 16S rRNA amplicons. An extremely complex ecosystem including taxa potentially contributing to both indigo reduction and form ...
... This study aimed to investigate the effect of acid- or alkali-treatment on volatile fatty acids (VFAs) production and microbiological mechanism during primary sludge anaerobic fermentation. Seven fermentation experiments were conducted at different pH (3−12). Results showed that the optimal pH was 10 for accumulation of VFAs. High-throughput sequencing results indicated that acid and alkali treatm ...
Erysipelothrix; Peptostreptococcaceae; Tissierella; acidification; activated sludge; calcium hydroxide; chemical oxygen demand; fermentation; long term effects; microbial communities; polysaccharides; potassium hydroxide; short chain fatty acids; sodium hydroxide; temperature
Abstract:
... The effect of four alkali reagents (NaOH, KOH, Ca(OH)2, mixed alkali) on waste activated sludge (WAS) hydrolytic acidification and microbial community was studied in semi-continuous fermentation systems at low temperature (15°C) over long term operational time (65day). The results showed that protein and polysaccharide of NaOH (124.26, 11.92) was similar to that of KOH (109.53, 11.30), both were h ...
Carnobacteriaceae; Raja; Tissierella; ammonia; community structure; fermentation; fish; gamma-Proteobacteria; lactic acid bacteria; markets; pH; prokaryotic cells; reverse transcriptase polymerase chain reaction; ribosomal RNA; salinity; sequence analysis; species diversity; urea; South Korea
Abstract:
... Prokaryotes were extracted from skates and fermented skates purchased from fish markets and a local manufacturer in South Korea. The prokaryotic community composition of skates and fermented skates was investigated using 16S rRNA pyrosequencing. The ranges for pH and salinity of the grinded tissue extract from fermented skates were 8.4–8.9 and 1.6–6.6%, respectively. Urea and ammonia concentration ...
... Increasing selenium concentrations in aquatic environments downstream of mine sites is of great concern due to selenium's bioaccumulation propensity and teratogenic toxicity. Removal of selenium from mine influenced water is complicated by the presence of nitrate, which is also elevated in mine influenced water due to the use of explosives in mining. In many biological treatment processes, nitrate ...
Lahr Rebecca H.; Goetsch Heather E.; Haig Sarah J.; Noe-Hays Abraham; Love Nancy G.; Aga Diana S.; Bott Charles B.; Foxman Betsy; Jimenez Jose; Luo Ting; Nace Kim; Ramadugu Kirtana; Wigginton Krista R.
... Source-separated human urine was collected from six public events to study the impact of urine processing and storage on bacterial community composition and viability. Illumina 16S rRNA gene sequencing revealed a complex community of bacteria in fresh urine that differed across collection events. Despite the harsh chemical conditions of stored urine (pH > 9 and total ammonia nitrogen > 4000 mg N/L ...
... This investigation provides generic information on the culturable corrosive and the microfouling bacterial community in a firewater distribution system that uses freshwater. Conventional microbiological methods were used for the selective isolation of the major microfouling bacteria. The isolates were characterized by 16S rRNA gene sequencing and the biofilm as well as the corrosion characteristic ...
... This study employed mixed-culture consolidated bioprocessing (CBP) to digest microalgal biomass in an anaerobic sequencing batch reactor (ASBR). The primary objectives are to evaluate the impact of hydraulic residence time (HRT) on the productivity of carboxylic acids and to characterize the bacterial community. HRT affects the production rate and patterns of carboxylic acids. For the 5-L laborato ...
... The study provided a cost-effective and high-efficiency volatile fatty acid (VFA) production strategy by co-fermentation of food waste (FW) and excess sludge (ES) without artificial pH control. VFA production of 867.42mg COD/g-VS was obtained under the optimized condition: FW/ES 5, solid retention time 7d, organic loading rate 9g VS/L-d and temperature 40°C. Mechanism exploration revealed that the ...
... A serial enrichment culture has been grown in an alkaline Fe(III)-citrate-containing medium from an initial inoculum from a soil layer beneath a chromium ore processing residue (COPR) disposal site where Cr(III) is accumulating from Cr(VI) containing leachate. This culture is dominated by two bacterial genera in the order Clostridiales, Tissierella, and an unnamed Clostridium XI subgroup. This pap ...
... Kusaya is a salted, dried fish product traditionally produced on the Izu Islands in Japan. Fish are added to kusaya gravy repeatedly and intermittently, and used over several hundred years, which makes unique microbiota and unique flavors. In this study, we performed a metagenomic analysis to compare the composition of the microbiota of kusaya gravy between different islands. Twenty samples obtain ...
... Sludge fermentation between pH 4 and 11 was investigated to generate volatile fatty acids (VFA). Despite the highest sludge solubilization of 25.9% at pH 11, VFA accumulation was optimized at pH 8 (12.5% out of 13.1% sludge solubilization). 454 pyrosequencing identified wide diversity of acidogens in bioreactors operated at the various pHs, with Tissierella, Petrimonas, Proteiniphilum, Levilinea, ...
... In this work bacteria and methanogenic archaea utilizing agricultural wastes in a pilot-scale biogas reactor were examined using sequencing and terminal restriction fragment length polymorphism analysis. Based on the analyses of 16S rRNA genes, Clostridia represented the most diverse group in the digester. Of the Clostridia, unclassified Clostridiales and the members of the genera Anaerotruncus an ...
... Vinyl chloride (VC), a known human carcinogen, is a common and persistent groundwater pollutant at many chlorinated solvent contaminated sites. The remediation of such sites is challenging because of the lack of knowledge on the microorganisms responsible for in situ VC degradation. To address this, the microorganisms involved in carbon assimilation from VC were investigated in a culture enriched ...
... AIMS: In the brewing industry, microbial management is very important for stabilizing the quality of the product. We investigated the detailed microbial community of beer during fermentation and maturation, to manage beer microbiology in more detail. METHODS AND RESULTS: We brewed a beer (all‐malt) and two beerlike beverages (half‐ and low‐malt) in pilot‐scale fermentation and investigated the mic ...
Clostridium; DNA primers; Halothermothrix; Roseburia; Tissierella; databases; fermentation; genes; population dynamics; ribosomal RNA
Abstract:
... In this study, a Clostridia-specific primer set SJ-F and SJ-R, based on the available 16S rRNA genes sequences from database, was successfully designed and authenticated by theoretical and experimental evaluations. It targeted 19 clostridial families and unclassified_Clostridia with different coverage rates. The specificity and universality of novel primer set was tested again using the dark ferme ...
... Biocathodes in bioelectrochemical systems (BESs) can be used to convert CO2 into diverse organic compounds through a process called microbial electrosynthesis. Unfortunately, start-up of anaerobic biocathodes in BESs is a difficult and time consuming process. Here, a pre-enrichment method was developed to improve start-up of anaerobic facultatively autotrophic biocathodes capable of using cathodes ...
... The purpose of this study was to characterize precipitates formed in anaerobic, H₂S-producing cultures of two Tissierella isolates and Desulfosporosinus strain DB. The cultures were grown in Cu-containing media as part of a larger study of Cu resistance in anaerobic sulfidogens. The Tissierella strains produced H₂S from peptone. Desulfosporosinus formed H₂S from peptone or through dissimilatory su ...