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- Author:
- McNeil, Heather J.; Shewen, Patricia E.; Lo, Reggie Y.C.; Conlon, Jennifer A.; Miller, Michael W.
- Source:
- Veterinary immunology and immunopathology 2002 v.90 no.1-2 pp. 107-110
- ISSN:
- 0165-2427
- Subject:
- Mannheimia haemolytica serotype 1; Pasteurella trehalosi; Western blotting; disease control; fibrinogen; pneumonia; serotypes; virulence
- Abstract:
- ... Fibrinogen-binding proteins were found in the culture supernatants of Mannheimia haemolytica serotype 1 (ATCC 43270) and Pasteurella trehalosi serotype 10 (ECO-100). Sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. Fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteris ...
- DOI:
- 10.1016/S0165-2427(02)00222-2
- https://doi.org/10.1016/S0165-2427(02)00222-2
- Author:
- Jaleel, Abdul; Halvatsiotis, Panagiotis; Williamson, Brian; Juhasz, Peter; Martin, Stephen; Nair, K. Sreekumaran
- Source:
- Diabetes care 2005 v.28 no.3 pp. 645-652
- ISSN:
- 0149-5992
- Subject:
- glycation; Western blotting; noninsulin-dependent diabetes mellitus; blood; two-dimensional gel electrophoresis; transferrin; binding proteins; vitamin D; patients; fibrin; lysine; albumins; monitoring; serine proteinases; blood glucose; insulin; body mass index; glycemic control; fibrinogen; tandem mass spectrometry
- Abstract:
- ... OBJECTIVE:--Growing evidence supports that nonenzymatic glycation products may cause hyperglycemia-induced diabetes complications. Amadori-modified proteins are the intermediate products of nonenzymatic glycation and constitute the forms of glycated proteins in diabetes. The objective of the current study was to utilize two-dimensional gel electrophoresis, Western blot, and mass spectrometry to id ...
- DOI:
- 10.2337/diacare.28.3.645
- PubMed:
- 15735202
- https://doi.org/10.2337/diacare.28.3.645
- Author:
- Li-Peng Xu; Tao Ma; Wei-Jian Jiang; Peng-Xin Qiu; Xia Wang; Xing-Wen Su; Guang-Mei Yan
- Source:
- Applied microbiology and biotechnology 2006 v.72 no.1 pp. 72-76
- ISSN:
- 0175-7598
- Subject:
- Agkistrodon; Western blotting; animals; fibrinogen; molecular weight; thrombin; venoms
- Abstract:
- ... A novel metalloproteinase, recombinant fibrinogenase IV (rFIVₐ), was expressed and purified from Agkistrodon acutus venom. It is a single-chain protein with an apparent molecular weight of 27 kDa. Western blot showed that it had a good immunological reaction against anti-FIVₐ rabbit serum. The kinetic parameters Kₘ and Kcₐₜ of rFIVₐ on the substrate T6140 were 7.471×10⁻⁴ mol/l and 5.103×10⁻⁵ s⁻¹. ...
- DOI:
- 10.1007/s00253-005-0254-9
- PubMed:
- 16429282
- https://doi.org/10.1007/s00253-005-0254-9
- Author:
- Chen, Hong-Sen; Chen, Jin-Mei; Lin, Chia-Wei; Khoo, Kay-Hooi; Tsai, Inn-Ho
- Source:
- FEBS journal 2008 v.275 no.15 pp. 3944-3958
- ISSN:
- 1742-464X
- Subject:
- Vipera; Western blotting; coagulation; complementary DNA; disseminated intravascular coagulation; epitopes; fibrinogen; glycoproteins; polysaccharides; receptors; sugars; venoms
- Abstract:
- ... The coagulation factor X activator from Russell's viper venom (RVV-X) is a heterotrimeric glycoprotein. In this study, its three subunits were cloned and sequenced from the venom gland cDNAs of Daboia siamensis. The deduced heavy chain sequence contained a C-terminal extension with four additional residues to that published previously. Both light chains showed 77-81% identity to those of a homolog ...
- DOI:
- 10.1111/j.1742-4658.2008.06540.x
- https://doi.org/10.1111/j.1742-4658.2008.06540.x
- Author:
- Majidzadeh-A, Keivan; Khalaj, Vahid; Fatemeh, Davami; Mahdi, Hemayatkar; Farzaneh, Barkhordari; Ahmad, Adeli; Mahboudi, Fereidoun
- Source:
- Applied biochemistry and biotechnology 2010 v.162 no.7 pp. 2037-2048
- ISSN:
- 0273-2289
- Subject:
- alcohol oxidase; Komagataella pastoris; secretion; polyacrylamide gel electrophoresis; Western blotting; genes; stroke; gelatin; t-plasminogen activator; myocardial infarction; disulfide bonds; thrombosis; signal peptide; plasmin; plasminogen; humans; yeasts; culture media; adverse effects; methanol; fibrinogen; hemorrhage; Saccharomyces cerevisiae
- Abstract:
- ... Human tissue plasminogen activator (t-PA) plays a pivotal role in the treatment of acute myocardial infarction, ischemic stroke, and deep vein thrombosis. It has the benefit of generating no adverse effects such as fibrinogen depletion, systemic hemorrhage, and immunologic reactions. Human t-PA is a serine-protease enzyme containing 527 amino acid residues in five structural domains. The correct f ...
- DOI:
- 10.1007/s12010-010-8979-z
- PubMed:
- 20455033
- https://doi.org/10.1007/s12010-010-8979-z
- Author:
- Pei-pei Lu; Jun-tian Liu; Na Liu; Fang Guo; Yuan-yuan Ji; Xiaoming Pang
- Source:
- Life sciences 2011 v.88 no.19-20 pp. 839-845
- ISSN:
- 0024-3205
- Subject:
- Western blotting; atherogenesis; atherosclerosis; enzyme-linked immunosorbent assay; fibrin; fibrinogen; gene expression; immunocytochemistry; inducible nitric oxide synthase; inflammation; interleukin-6; messenger RNA; myocytes; pathogenesis; protein synthesis; rats; reverse transcriptase polymerase chain reaction; smooth muscle; tumor necrosis factor-alpha
- Abstract:
- ... AIMS: Atherosclerosis is a chronic inflammatory response of the arterial wall to multiple endothelial injuries. As one of the inflammatory markers, fibrinogen has been implicated in pathogenesis of atherosclerosis. But, it is not completely understood whether atherogenesis of fibrinogen is related to its pro-inflammatory effect on vascular smooth muscle cells (VSMCs). The purpose of the present st ...
- DOI:
- 10.1016/j.lfs.2011.03.003
- https://doi.org/10.1016/j.lfs.2011.03.003
- Author:
- S. Talens; J.H.G. Lebbink; J.J.M.C. Malfliet; J.A.A. Demmers; S. Uitte de Willige; F.W.G. Leebeek; D.C. Rijken
- Source:
- Biochemical and biophysical research communications 2012 v.427 no.2 pp. 421-425
- ISSN:
- 0006-291X
- Subject:
- Western blotting; arginine; blood coagulation; calcium chloride; fibrin; fibrinogen; fibrinolysis; lysine; mass spectrometry; proteolysis; surface plasmon resonance; thrombin; two-dimensional gel electrophoresis
- Abstract:
- ... The ultimate step in the blood coagulation cascade is the formation of fibrin. Several proteins are known to bind to fibrin and may thereby change clot properties or clot function. Our previous studies identified carboxypeptidase N (CPN) as a novel plasma clot component. CPN cleaves C-terminal lysine and arginine residues from several proteins. The activity of CPN is increased upon its proteolysis ...
- DOI:
- 10.1016/j.bbrc.2012.09.081
- https://doi.org/10.1016/j.bbrc.2012.09.081
- Author:
- Jae Hong Park; Jung Min Ryu; Seung Pil Yun; Mi Ok Kim; Ho Jae Han
- Source:
- Biochimica et biophysica acta 2012 v.1820 no.10 pp. 1618-1627
- ISSN:
- 0304-4165
- Subject:
- CD29 antigen; Western blotting; actin; calcium; cell movement; collagen; embryonic stem cells; extracellular matrix; fibrinogen; fibronectins; gelatinase A; laminin; messenger RNA; mice; mitogen-activated protein kinase; pH; phosphorylation; plasma membrane; precipitin tests; protein synthesis; signal transduction; small interfering RNA; sodium-hydrogen antiporter
- Abstract:
- ... BACKGROUND: Extracellular matrix (ECM) components and intracellular pH (pHᵢ) may serve as regulators of cell migration in various cell types. METHODS: The Oris migration assay was used to assess the effect of fibronectin (FN) on cell motility. The Na⁺/H⁺ exchanger (NHE)-1 activity was evaluated by measuring pHᵢ and [²²Na⁺] uptake. To examine activated signaling molecules, western blot analysis and ...
- DOI:
- 10.1016/j.bbagen.2012.05.013
- https://doi.org/10.1016/j.bbagen.2012.05.013
- Author:
- Sanjay Kumar; Anil Kumar Tomar; Sudhuman Singh; Mayank Saraswat; Sarman Singh; Tej P. Singh; Savita Yadav
- Source:
- International journal of biological macromolecules 2012 v.50 no.2 pp. 317-322
- ISSN:
- 0141-8130
- Subject:
- Western blotting; actin; anti-infective properties; apoptosis; chromatography; fibrinogen; glycoproteins; human serum albumin; humans; hydrogen bonding; immunoglobulin G; immunomodulation; keratin; light scattering; male fertility; matrix-assisted laser desorption-ionization mass spectrometry; myosin; polyacrylamide gel electrophoresis; prolactin; seminal plasma; sperm motility
- Abstract:
- ... Prolactin inducible protein (PIP) is a 17kDa glycoprotein. It binds to many proteins including fibrinogen, actin, keratin, myosin, immunoglobulin G, CD4, and human zinc-alpha-2 glycoprotein. Its ability to bind a large array of proteins indicates its multifaceted role in various biological processes, such as fertility, immunoregulation, antimicrobial activity, apoptosis, and tumor progression. Her ...
- DOI:
- 10.1016/j.ijbiomac.2011.12.015
- https://doi.org/10.1016/j.ijbiomac.2011.12.015
- Author:
- Sask Kyla N.; Berry Leslie R.; Chan Anthony K.C.; Brash John L.
- Source:
- Langmuir 2012 v.28 no.4 pp. 2099-2106
- ISSN:
- 1520-5827
- Subject:
- Western blotting; X-ray photoelectron spectroscopy; adsorption; anticoagulant activity; blood platelets; cell adhesion; contact angle; fibrinogen; heparin; molecular weight; polyethylene glycol; polyurethanes
- Abstract:
- ... Polyurethane (PU) was modified using isocyanate chemistry to graft polyethylene oxide (PEO) of various molecular weights (range 300–4600). An antithrombin–heparin (ATH) covalent complex was subsequently attached to the free PEO chain ends, which had been functionalized with N-hydroxysuccinimide (NHS) groups. Surfaces were characterized by water contact angle and X-ray photoelectron spectroscopy (X ...
- DOI:
- 10.1021/la203821g
- https://doi.org/10.1021/la203821g
- Author:
- Hao Liu; Peter S. Yang; Tina Zhu; Justin Manuel; Jianhua Zhang; Wei He; Itay Shalev; Li Zhang; Myron I. Cybulsky; David R. Grant; M. James Phillips; Gary A. Levy
- Source:
- international journal of biochemistry & cell biology 2013 v.45 no.2 pp. 408-418
- ISSN:
- 1357-2725
- Subject:
- T-lymphocytes; Western blotting; antigen-presenting cells; binding capacity; cysteine; fibrinogen; innate immunity; lectins; mice; molecular weight; mutation; pathogenesis; receptors; rheumatoid arthritis; solubility; structure-activity relationships; sugars; therapeutics; viral hepatitis
- Abstract:
- ... Fibrinogen-like protein 2 (FGL2), a novel effector molecule of CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Treg), mediates its suppressive activity through binding to low affinity Fcγ receptors expressed on antigen presenting cells (APCs). FGL2 has been implicated in the pathogenesis of viral hepatitis, xeno- and allotransplant rejection, and rheumatoid arthritis. Here we fully analyzed the structure–func ...
- DOI:
- 10.1016/j.biocel.2012.10.014
- https://doi.org/10.1016/j.biocel.2012.10.014
- Author:
- Mingjuan Sun#; Rongjiang Zou#; Xiaoyi Dong; Ying Zong; Yun Gao; Lianghua Wang; Binghua Jiao
- Source:
- Preparative biochemistry & biotechnology 2013 v.43 no.3 pp. 315-327
- ISSN:
- 1532-2297
- Subject:
- Western blotting; affinity chromatography; amylose; antibody specificity; biotechnology; blood vessels; cell proliferation; endothelial cells; enzyme-linked immunosorbent assay; fibrinogen; genes; humans; immunization; immunocytochemistry; lung neoplasms; mice; polyclonal antibodies; rapid methods; sequence homology
- Abstract:
- ... Angiopoietin-related protein 1 (ARP1) is one of the antiangiogenic factors and plays an important role in endothelial cell proliferation, migration, and blood vessel network formation. Here a rapid method to prepare ARP1 polyclonal antibody in 1 month was developed. The gene of fibrinogen homology domain (FD) for ARP1 was cloned and the protein was expressed in a soluble form of MBP-FD fused prote ...
- DOI:
- 10.1080/10826068.2012.746231
- https://doi.org/10.1080/10826068.2012.746231
- Author:
- Li Guanghui; Qiao Mingyu; Guo Yan; Wang Xin; Xu Yunfeng; Xia Xiaodong
- Source:
- Foodborne pathogens & disease 2014 v.11 no.9 pp. 677-683
- ISSN:
- 1556-7125
- Subject:
- Staphylococcus aureus; Western blotting; chlorogenic acid; fibrinogen; genes; hemolysins; hemolysis; minimum inhibitory concentration; pathogens; reverse transcriptase polymerase chain reaction; staphylococcal intoxication; virulence
- Abstract:
- ... Chlorogenic acid (CA) has been reported to inhibit several pathogens, but the influence of subinhibitory concentrations of CA on virulence expression of pathogens has not been fully elucidated. The aim of this study was to explore the effect of CA on the virulence factor production of Staphylococcus aureus. The minimum inhibitory concentration (MIC) of CA against S. aureus was determined using a b ...
- DOI:
- 10.1089/fpd.2013.1731
- https://doi.org/10.1089/fpd.2013.1731
- Author:
- C. Klein
- Source:
- Domestic animal endocrinology 2016 v.56 pp. S212
- ISSN:
- 0739-7240
- Subject:
- Western blotting; angiogenesis; conceptus; corpus luteum; culture media; early development; endometrium; estrogens; fibrinogen; gene expression regulation; glycoproteins; high-throughput nucleotide sequencing; humans; immunohistochemistry; integrins; luteolysis; mares; mice; ovulation; polysaccharides; pregnancy; prostaglandins; receptors; relaxin; reverse transcriptase polymerase chain reaction; secretion; sialic acids; sialidase; signal transduction; staining; swine
- Abstract:
- ... “Maternal recognition of pregnancy” (MRP) is commonly used to describe the ongoing embryo-maternal communication during early pregnancy that culminates in prevention of luteolysis and ensures ongoing progestin support. The conceptus-derived pregnancy recognition signal has not yet been identified in the mare. Although equine conceptuses produce substantial amounts of estrogens, there is a lack of ...
- DOI:
- 10.1016/j.domaniend.2016.03.006
- https://doi.org/10.1016/j.domaniend.2016.03.006
- Author:
- Shobana Ponnuvel; Dhanalakshmi Bandaru; Preethi Ragunathan; Karthe Ponnuraj
- Source:
- RSC advances 2016 v.6 no.94 pp. 91824-91835
- ISSN:
- 2046-2069
- Subject:
- Gram-positive bacteria; Streptococcus agalactiae; Streptococcus pneumoniae; Streptococcus pyogenes; Western blotting; adhesins; binding properties; binding proteins; circular dichroism spectroscopy; enzyme-linked immunosorbent assay; fibrinogen; fibronectins; inclusion bodies; interferometry; ligands; prediction; sequence analysis; solubilization; surface proteins; urea; virulence
- Abstract:
- ... Pneumococcal adherence and virulence factor A (PavA) were first identified in Streptococcus pneumoniae as an adhesin binding to fibronectin and studies suggested that it is an important virulence determinant. Homologs of PavA are found in many Gram-positive bacteria including S. agalactiae. However, to date, the details of its structure or mode of interaction with the host molecule(s) are not know ...
- DOI:
- 10.1039/c6ra18275e
- https://doi.org/10.1039/c6ra18275e
- Author:
- María Elisa Peichoto; Marcelo Larami Santoro
- Source:
- Toxicon 2016 v.119 pp. 330-335
- ISSN:
- 0041-0101
- Subject:
- Western blotting; antivenoms; bioactive properties; blood; blood cell counts; coagulation; fibrinogen; hemostasis; immunohistochemistry; injection site; metalloproteinases; protein disulfide-isomerase; protein synthesis; rats; snakes; therapeutics; venoms
- Abstract:
- ... Patagonfibrase is a hemorrhagic metalloproteinase isolated from the venom of the South American rear-fanged snake Philodryas patagoniensis, and is an important contributor to local lesions inflicted by this species. The tissue factor (TF)-factor VIIa complex, besides triggering the coagulation cascade, has been demonstrated to be involved in inflammatory events. Our aim was to determine whether pa ...
- DOI:
- 10.1016/j.toxicon.2016.07.001
- https://doi.org/10.1016/j.toxicon.2016.07.001
- Author:
- Mathia Colwell; Najma Ahmed; Ralph Butkowski
- Source:
- Analytical biochemistry 2017 v.525 pp. 67-72
- ISSN:
- 0003-2697
- Subject:
- Western blotting; alkaline phosphatase; ammonium sulfate; antibodies; cattle; chickens; disulfide bonds; enzyme-linked immunosorbent assay; fibrinogen; fractionation; glycoproteins; guinea pigs; humans; ion exchange chromatography; nickel; peroxidase; rabbits
- Abstract:
- ... Nickel-bound alkaline phosphatase and peroxidase enzymes were used to investigate nickel binding to plasma proteins. Rabbit plasma dilutions to 25,000 were positive by ELISA, while Western blot analysis showed a prominent reaction with histidine-rich glycoprotein (HRG)1 and lower reaction with fibrinogen (Fgn). To confirm their identities, purified HRG and Fgn were demonstrated to react with the n ...
- DOI:
- 10.1016/j.ab.2017.02.013
- https://doi.org/10.1016/j.ab.2017.02.013
- Author:
- Madison Marcinczyk; Hady Elmashhady; Muhamed Talovic; Andrew Dunn; Faiz Bugis; Koyal Garg
- Source:
- Biomaterials 2017 v.141 pp. 233-242
- ISSN:
- 0142-9612
- Subject:
- Western blotting; desmin; fibrin; fibrinogen; hydrogels; insulin-like growth factor I; interleukin-6; laminin; models; modulus of elasticity; muscle development; myoblasts; myogenin; skeletal muscle; vascular endothelial growth factors; viscoelasticity
- Abstract:
- ... Laminin (LM)-111 supplementation has improved muscle regeneration in several models of disease and injury. This study investigated a novel hydrogel composed of fibrinogen and LM-111. Increasing LM-111 concentration (50–450 μg/mL) in fibrin hydrogels resulted in highly fibrous scaffolds with progressively thinner interlaced fibers. Rheological testing showed that all hydrogels had viscoelastic beha ...
- DOI:
- 10.1016/j.biomaterials.2017.07.003
- https://doi.org/10.1016/j.biomaterials.2017.07.003
- Author:
- Ya-Li Shiu; Kuo-Hsun Chiu; Truong-Giang Huynh; Ping-Chung Liu; Chun-Hung Liu
- Source:
- Fish & shellfish immunology 2017 v.65 pp. 235-243
- ISSN:
- 1050-4648
- Subject:
- Epinephelus coioides; Vibrio alginolyticus; Western blotting; antibodies; bacterial infections; choline; complement; diet; disease resistance; enzyme activity; fibrinogen; fish meal; grouper; immune response; immunoglobulins; innate immunity; ionization; kidneys; liver; mass spectrometry; messenger RNA; protein synthesis; proteomics; quantitative polymerase chain reaction; spleen; two-dimensional gel electrophoresis
- Abstract:
- ... This study aimed to unravel the regulatory roles of choline in activating immune responses and disease resistance of the orange-spotted grouper Epinephelus coioides. Fish were fed a choline-supplemented diet at 1 g kg−1 of feed for 30 days. Fish fed a fish meal basal diet without choline-supplement served as controls. At the end of the feeding trial, fish were challenged with Vibrio alginolyticus. ...
- DOI:
- 10.1016/j.fsi.2017.04.022
- https://doi.org/10.1016/j.fsi.2017.04.022
- Author:
- Graziano Colombo; Marco Clerici; Alessandra Altomare; Francesco Rusconi; Daniela Giustarini; Nicola Portinaro; Maria Lisa Garavaglia; Ranieri Rossi; Isabella Dalle-Donne; Aldo Milzani
- Source:
- Journal of proteomics 2017 v.152 pp. 22-32
- ISSN:
- 1874-3919
- Subject:
- Western blotting; apolipoprotein A-I; biomarkers; blood; complement; crosslinking; derivatization; disease course; fibrinogen; fluorescence; gel chromatography; haptoglobins; high performance liquid chromatography; human diseases; humans; matrix-assisted laser desorption-ionization mass spectrometry; molecular weight; myeloperoxidase; oxidation; polyacrylamide gel electrophoresis; prealbumin; protein aggregates; protein composition; proteomics; tandem mass spectrometry; thiols
- Abstract:
- ... In this study, we assessed the oxidative damage occurring in plasma proteins when human blood was exposed to inflammatory concentrations of hypochlorous acid (HOCl). We used specific thiol labelling and Western blot analyses to determine protein thiol oxidation, as well as analytical gel filtration HPLC coupled to fluorescence detection to explore formation of high molecular weight (HMW) protein a ...
- DOI:
- 10.1016/j.jprot.2016.10.008
- https://doi.org/10.1016/j.jprot.2016.10.008