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You searched for: Subject Western blotting Remove constraint Subject: Western blotting Subject fibrinogen Remove constraint Subject: fibrinogen
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1. Identification of novel biomarkers of inflammation in Atlantic salmon (Salmo salar L.) by a plasma proteomic approach

Author:
Baojian Sun; Dino van Dissel; Ingrid Mo; Preben Boysen; Hanne Haslene-Hox; Hege Lund
Source:
Developmental and comparative immunology 2022 v.127 pp. 104268
ISSN:
0145-305X
Subject:
Aeromonas salmonicida subsp. salmonicida; Salmo salar; Toll-like receptor 5; Western blotting; amyloid; animal welfare; aquaculture industry; bacterial infections; biomarkers; blood serum; cathelicidins; complement; fibrinogen; fish; fish health; haptoglobins; immunoglobulins; inflammation; liver; mass spectrometry; monitoring; phospholipase A2; proteomics; quantitative polymerase chain reaction; spleen
Abstract:
... Monitoring fish welfare has become a central issue for the fast-growing aquaculture industry, and finding proper biomarkers of stress, inflammation and infection is necessary for surveillance and documentation of fish health. In this study, a proteomic approach using mass spectrometry was applied to identify indicators of the acute response in Atlantic salmon blood plasma by comparing Aeromonas sa ...
DOI:
10.1016/j.dci.2021.104268

2. The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1α and VEGF via the PI3K/AKT and ERK1/2 signaling pathways

Author:
Yan LI; Jing-Ping GE; Ke MA; Yuan-Yuan YIN; Juan HE; Jian-Ping GU
Source:
Chinese journal of natural medicines 2022 v.20 no.9 pp. 679-690
ISSN:
1875-5364
Subject:
Western blotting; anticoagulants; apoptosis; blood; coagulation; endothelial cells; epigallocatechin gallate; fibrinogen; flow cytometry; fluorescent antibody technique; histopathology; hydrogen peroxide; immunohistochemistry; inferior vena cava; models; oxidation; oxidative stress; polyphenols; protein synthesis; prothrombin; public health; tea; thrombin; thromboplastin; thrombosis; viability; warfarin
Abstract:
... Deep venous thrombosis (DVT) poses a major challenge to public health worldwide. Endothelial cell injury evokes inflammatory and oxidative responses that contribute to thrombus formation. Tea polyphenol (TP) in the form of epigallocatechin-3-gallate (EGCG) has anti-inflammatory and oxidative effect that may ameliorate DVT. However, the precise mechanism remains incompletely understood. The current ...
DOI:
10.1016/S1875-5364(22)60172-9

3. The Effects of Prunus spinosa L. Flower Extracts, Model Polyphenols and Phenolic Metabolites on Oxidative/Nitrative Modifications of Human Plasma Components with Particular Emphasis on Fibrinogen In Vitro

Author:
Anna Marchelak; Joanna Kolodziejczyk-Czepas; Paulina Wasielewska; Pawel Nowak; Monika A. Olszewska
Source:
Antioxidants 2021 v.10 no.4 pp. -
ISSN:
2076-3921
Subject:
Prunus spinosa; Western blotting; adjuvants; antioxidant activity; blood plasma; chemical species; fibrinogen; flowers; fluorometry; hemostasis; herbal medicines; humans; metabolites; molecular weight; oxidation; oxidative stress; pathogenesis; polyacrylamide gel electrophoresis; polyphenols; tryptophan; tyrosine
Abstract:
... Oxidative post-translational modifications of fibrinogen (a multifunctional blood plasma protein essential for hemostasis) are associated with the pathogenesis of cardiovascular disorders (CVDs). Prunus spinosa flower is a herbal medicine used in an adjuvant treatment of CVDs and rich in polyphenolic antioxidants. In the present study, phytochemically standardized P. spinosa flower extracts, their ...
DOI:
10.3390/antiox10040581

4. Theaflavin: a natural candidate to restrain thrombosis

Author:
Gang Zhang; Yani Pan; Hao Cheng; Shuying Gong; Qiang Chu; Ping Chen
Source:
Food & function 2022 v.13 no.14 pp. 7572-7581
ISSN:
2042-650X
Subject:
Western blotting; black tea; dose response; enzyme-linked immunosorbent assay; fibrinogen; flow cytometry; fluorescence microscopy; humans; models; non-specific protein-tyrosine kinase; phosphorylation; platelet aggregation; tail; theaflavins; thrombosis; thromboxanes
Abstract:
... Many clinical studies have demonstrated the beneficial effects of black tea on cardiovascular diseases. However, the antiplatelet and antithrombotic activities of theaflavin (TF-1) remain unknown. In this study, we aimed to investigate the beneficial effects of TF-1 on platelet activation and thrombosis formation both in vitro and in vivo. Firstly, the in vitro antiplatelet activity of TF-1 was an ...
DOI:
10.1039/d2fo00152g

5. Anti-thrombotic activity of phenolic acids obtained from Salvia miltiorrhiza f. alba in TNF-α-stimulated endothelial cells via the NF-κB/JNK/p38 MAPK signaling pathway

Author:
Xianjing Zheng; Haimei Liu; Maoqiang Ma; Jianbo Ji; Faliang Zhu; Longru Sun
Source:
Archives of pharmacal research 2021 v.44 no.4 pp. 427-438
ISSN:
0253-6269
Subject:
Salvia miltiorrhiza; Western blotting; adenosine diphosphate; blood; enzyme-linked immunosorbent assay; fibrinogen; platelet aggregation; prothrombin; quantitative polymerase chain reaction; rats; research; salvianolic acid; thromboplastin; thrombosis
Abstract:
... Over the past 100 years, Salvia miltiorrhiza f. alba (Lamiaceae) (RSMA) roots have been used to cure thromboangiitis obliterans (TAO) in local clinics. This study aimed to confirm the anti-thrombotic efficacy of 12 phenolic acids obtained from RSMA and to clarify the possible underlying mechanisms. The results of quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunoso ...
DOI:
10.1007/s12272-021-01325-7

6. The Effects of Sorbus aucuparia L. Fruit Extracts on Oxidative/Nitrative Modifications of Human Fibrinogen, Impact on Enzymatic Properties of Thrombin, and Hyaluronidase Activity In Vitro

Author:
Magdalena Rutkowska; Joanna Kolodziejczyk-Czepas; Monika Anna Olszewska
Source:
Antioxidants 2021 v.10 no.12 pp. -
ISSN:
2076-3921
Subject:
Sorbus aucuparia; Western blotting; bioactive properties; coumaric acids; diabetes; enzyme activity; fibrinogen; flavanols; flavonols; fruits; health promotion; hemostasis; humans; hyaluronoglucosaminidase; nutritive value; oxidative stress; polyacrylamide gel electrophoresis; proanthocyanidins; proteolysis; synergism; thrombin; tyrosine
Abstract:
... Sorbus aucuparia L. fruits (rowanberries) are food products with acknowledged nutritional value, high phenolic content, and traditional application in diabetes. In this study, the effects of rowanberry extracts (phytochemically standardised, i.a., by LC-MS/MS) on some aspects of plasma haemostasis and vascular conditions were evaluated in vitro as possible mechanisms connected with cardiovascular ...
DOI:
10.3390/antiox10122009

7. Label-free proteomic analysis reveals the differentiation between unfertilized and fertilized Beijing-You chicken eggs

Author:
Lijing Zhang; Jilan Chen; Bei Fan; Mai Fu; Yanyan Sun; Yan Wang; Fengzhong Wang
Source:
International journal of biological macromolecules 2020 v.152 pp. 1020-1026
ISSN:
0141-8130
Subject:
Western blotting; apolipoprotein B; chicken eggs; eggs; embryo (animal); embryogenesis; fibrinogen; gene expression regulation; gene ontology; inflammation; liquid chromatography; messenger RNA; phospholipids; protein synthesis; proteomics; reverse transcriptase polymerase chain reaction; tandem mass spectrometry; transferrin receptors; vimentin
Abstract:
... Egg fertilization is a dynamic process, including varieties of biochemical changes. To better understand the molecular mechanisms during the egg embryo development, the objective of this study was to quantify protein expression changes between fertilized and unfertilized Beijing-You chicken eggs using label-free liquid chromatography-tandem mass spectrometry method. The results showed that a total ...
DOI:
10.1016/j.ijbiomac.2019.10.189

8. Analyzing the adhesion mechanism of FnBPA, a surface adhesin from Staphylococcus aureus on its interaction with nanoparticle

Author:
Shobana Ponnuvel; Sreejanani Sankar; Karthe Ponnuraj
Source:
Microbial pathogenesis 2020 v.146 pp. 104239
ISSN:
0882-4010
Subject:
Staphylococcus aureus; Western blotting; absorption; adhesins; adhesion; bacteria; binding capacity; binding proteins; binding sites; circular dichroism spectroscopy; coatings; disease diagnosis; drugs; enzyme-linked immunosorbent assay; extracellular matrix; fibrinogen; fibronectins; gel chromatography; gel electrophoresis; interferometry; ligands; nanoparticles; nanosilver; protein corona; virulent strains
Abstract:
... Staphylococcus aureus expresses many Microbial Surface Recognizing Adhesive Matrix Molecules (MSCRAMM's) to recognize host extracellular matrix (ECM) molecules to initiate colonization. The MSCRAMM, fibronectin binding protein A (FnBPA), is an important adhesin for S. aureus infection. FnBPA also binds with fibrinogen (Fg) by using a unique ligand binding mechanism called dock, lock and latch. Nan ...
DOI:
10.1016/j.micpath.2020.104239

9. Venom Composition in a Phenotypically Variable Pit Viper (Trimeresurus insularis) across the Lesser Sunda Archipelago

Author:
Brenda Kathryn Jones; Anthony J. Saviola; Sean B. Reilly; Alexander L. Stubbs; Evy Arida; Djoko T. Iskandar; Jimmy A. McGuire; John R. Yates; Stephen P. Mackessy
Source:
Journal of proteome research 2019 v.18 no.5 pp. 2206-2220
ISSN:
1535-3907
Subject:
Trimeresurus albolabris; Western blotting; aminopeptidases; antivenoms; cobra venoms; enzyme activity; fibrinogen; gelatin; geographical isolation; hyaluronoglucosaminidase; islands; lectins; lysophospholipase; metalloproteinases; phenotype; phospholipases; polyacrylamide gel electrophoresis; proteome; reversed-phase high performance liquid chromatography; serine proteinases; vascular endothelial growth factors; Indonesia; Pacific Ocean Islands; Timor
Abstract:
... The genus Trimeresurus comprises a group of venomous pitvipers endemic to Southeast Asia and the Pacific Islands. Of these, Trimeresurus insularis, the White-lipped Island Pitviper, is a nocturnal, arboreal species that occurs on nearly every major island of the Lesser Sunda archipelago. In the current study, venom phenotypic characteristics of T. insularis sampled from eight Lesser Sunda Islands ...
DOI:
10.1021/acs.jproteome.9b00077

10. Attenuation of thrombin-mediated fibrin formation via changes in fibrinogen conformation induced by reaction with S-nitroso-N-acetylpenicillamine, but not S-nitrosoglutathione

Author:
Terry C. Major; Elizabeth J. Brisbois; Mark E. Meyerhoff; Robert H. Bartlett
Source:
Journal of materials chemistry B 2018 v.6 no.47 pp. 7954-7965
ISSN:
2050-7518
Subject:
Western blotting; blood platelets; fibrin; fibrinogen; fluorescence; glutathione; glycoproteins; high performance liquid chromatography; humans; in vitro studies; models; nitric oxide; nitroprusside; platelet aggregation; polymerization; polymers; rabbits; thrombin; tryptophan; tyrosine
Abstract:
... Previous work using a 4 h rabbit thrombogenicity model has shown that a nitric oxide (NO)-generating polymer extracorporeal circuit (ECC) with infusion of S-nitroso-N-acetyl-penicillamine (SNAP) preserved platelets even though platelets were activated, as shown by an increase in the glycoprotein p-selectin. The platelet preservation mechanism was shown to be due to a changing fibrinogen structure ...
DOI:
10.1039/c8tb02103a
PubMed:
31372222
PubMed Central:
PMC6675453

11. Elevated plasma levels of hepatocyte growth factor in rats experimentally envenomated with Bothrops jararaca venom: Role of snake venom metalloproteases

Author:
B.C. Prezoto; E.E. Kato; L.R.C. Gonçalves; S.C. Sampaio; I.S. Sano-Martins
Source:
Toxicon 2019 v.162 pp. 9-14
ISSN:
0041-0101
Subject:
Bothrops jararaca; EDTA (chelating agent); Western blotting; adults; blood coagulation; blood sampling; carotid arteries; coagulation; colorimetry; disseminated intravascular coagulation; enzyme-linked immunosorbent assay; fibrin; fibrinogen; genetic markers; hepatocyte growth factor; laboratory animals; males; metalloproteinases; polymerization; prothrombin; rats; snake venoms; snakes; sodium chloride; thrombin
Abstract:
... The hepatocyte growth factor (HGF)/c-met pathway, which mainly consists of HGF activator (HGFA) and its substrate HGF, protects various types of cells via anti-apoptotic and anti-inflammatory signals. Thrombin is the main physiological activator of such plasmatic pathway, and increased plasma concentrations of HGF have been considered as a molecular marker for some pathological conditions, such as ...
DOI:
10.1016/j.toxicon.2019.03.003

12. Fibrin scaffold could promote survival of the human adipose-derived stem cells during differentiation into cardiomyocyte-like cells

Author:
Zahra Bagheri-Hosseinabadi; Seyed Alireza Mesbah-Namin; Parvin Salehinejad; Fatemeh Seyedi
Source:
Cell and tissue research 2018 v.372 no.3 pp. 571-589
ISSN:
0302-766X
Subject:
Western blotting; cell differentiation; fibrin; fibrinogen; fluorescent antibody technique; genes; humans; immunochemistry; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; stem cells
Abstract:
... Human adipose-derived stem cells (hADSCs) are capable of differentiation into many cells including cardiac cells. Different types of scaffolds are used for cell differentiation but the best is yet to be determined. In this study, fibrin scaffold (3D) was fabricated using human plasma fibrinogen and compared with culture plates (2D) for the growth and differentiation of hADSCs into cardiomyocyte-li ...
DOI:
10.1007/s00441-018-2799-9

13. Identification of serum biomarkers for premature ovarian failure

Author:
Da-Hye Lee; Chang-Zhu Pei; Jae-Yun Song; Kyung-Ju Lee; Bo-Seong Yun; Kyu-Bum Kwack; Eun-Il Lee; Kwang-Hyun Baek
Source:
Biochimica et biophysica acta 2019 v.1867 no.3 pp. 219-226
ISSN:
1570-9639
Subject:
Western blotting; animal ovaries; biomarkers; blood serum; complement; enzyme-linked immunosorbent assay; females; ferroxidase; fibrinogen; gene expression regulation; liquid chromatography; menopause; pathogenesis; patients; proteomics; tandem mass spectrometry; two-dimensional gel electrophoresis
Abstract:
... Premature ovarian failure (POF) is defined when a female achieves menopause before the age of 40. Although many conditions are known to be causative for POF, the most common one is idiopathic. This study was undertaken to investigate the pathogenesis of POF using proteomic tools. Two-dimensional electrophoresis (2-DE) analysis was performed to screen for proteins differentially expressed in patien ...
DOI:
10.1016/j.bbapap.2018.12.007

14. In vitro large scale production of megakaryocytes to functional platelets from human hematopoietic stem cells

Author:
Pasupuleti Santhosh Kumar; Chodimella Chandrasekhar; Lokanathan Srikanth; Potukuchi Venkata Gurunadha Krishna Sarma
Source:
Biochemical and biophysical research communications 2018 v.505 no.1 pp. 168-175
ISSN:
0006-291X
Subject:
Andrographis paniculata; Western blotting; andrographolide; autografting; blood platelets; fibrinogen; flow cytometry; hematopoietic stem cells; hemostasis; humans; immunocytochemistry; liver; megakaryocytes; polyploidy; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; scanning electron microscopy; thrombin; thrombocytopenia; thrombopoietin
Abstract:
... Megakaryocytopoiesis results in the formation of platelets, which are essential for hemostasis. Decreased production or increased destruction of platelets can cause thrombocytopenia, in which platelet transfusion is the mode of treatment. The present study is aimed in generation of megakaryocytes (MKs) and platelet from human hematopoietic stem cells (HSCs). The purity of HSCs was assessed through ...
DOI:
10.1016/j.bbrc.2018.09.090

15. Transcriptome sequencing of Eospalax fontanierii to determine hypoxia regulation of cardiac fibrinogen

Author:
Lulu Xu; Zhiqiang Hao; Jinyan Lin; Qiong Zhao; Baojun Zhang; Guanglin Li; Jianping He; Jingang Li
Source:
Molecular biology reports 2019 v.46 no.6 pp. 5671-5683
ISSN:
0301-4851
Subject:
Western blotting; adults; blood viscosity; coagulation; fibrinogen; gene expression regulation; heart; hypoxia; molecular biology; oxygen; probability; quantitative polymerase chain reaction; thrombosis; transcriptome
Abstract:
... With the increase in blood viscosity, the blood circulation resistance will increase when animals are in hypoxia. However, these phenomenons do not appear in hypoxic-adapted animals. Eospalax fontanierii is a subterranean rodent and is an ideal species for research in hypoxia adaptation. Eighteen healthy adult E. fontanierii individuals were equally divided into three groups that were exposed to 2 ...
DOI:
10.1007/s11033-019-04690-1

16. The effect of sodium valproate on differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in two-dimensional culture and fibrin scaffold conditions

Author:
Hamid Najafipour; Zahra Bagheri-Hosseinabadi; Touba Eslaminejad; Hamid Reza Mollaei
Source:
Cell and tissue research 2019 v.378 no.1 pp. 127-141
ISSN:
0302-766X
Subject:
GATA transcription factors; Western blotting; cardiomyocytes; encapsulation; fibrin; fibrinogen; gene expression; genes; histone deacetylase; humans; immunochemistry; mesenchymal stromal cells; messenger RNA; protein content; stem cells
Abstract:
... Differentiation of mesenchymal stem cells (MSCs) into cardiomyocytes is a complex phenomenon, and attempts to find an effective inducing agent are still ongoing. We studied the effect of fibrin scaffold and sodium valproate (VPA, as a histone deacetylase inhibitor) on the differentiation of human adipose-derived stem cells (hADSCs) into cardiomyocyte-like cells. The cells were cultured in culture ...
DOI:
10.1007/s00441-019-03027-5

17. Detection of histidine-rich glycoprotein and fibrinogen with nickel-enzyme conjugates: Purification of rabbit HRG

Author:
Mathia Colwell; Najma Ahmed; Ralph Butkowski
Source:
Analytical biochemistry 2017 v.525 pp. 67-72
ISSN:
0003-2697
Subject:
Western blotting; alkaline phosphatase; ammonium sulfate; antibodies; cattle; chickens; disulfide bonds; enzyme-linked immunosorbent assay; fibrinogen; fractionation; glycoproteins; guinea pigs; humans; ion exchange chromatography; nickel; peroxidase; rabbits
Abstract:
... Nickel-bound alkaline phosphatase and peroxidase enzymes were used to investigate nickel binding to plasma proteins. Rabbit plasma dilutions to 25,000 were positive by ELISA, while Western blot analysis showed a prominent reaction with histidine-rich glycoprotein (HRG)1 and lower reaction with fibrinogen (Fgn). To confirm their identities, purified HRG and Fgn were demonstrated to react with the n ...
DOI:
10.1016/j.ab.2017.02.013

18. Plasma immune protein analysis in the orange-spotted grouper Epinephelus coioides: Evidence for altered expressions of immune factors associated with a choline-supplemented diet

Author:
Ya-Li Shiu; Kuo-Hsun Chiu; Truong-Giang Huynh; Ping-Chung Liu; Chun-Hung Liu
Source:
Fish & shellfish immunology 2017 v.65 pp. 235-243
ISSN:
1050-4648
Subject:
Epinephelus coioides; Vibrio alginolyticus; Western blotting; antibodies; bacterial infections; choline; complement; diet; disease resistance; enzyme activity; fibrinogen; fish meal; grouper; immune response; immunoglobulins; innate immunity; ionization; kidneys; liver; mass spectrometry; messenger RNA; protein synthesis; proteomics; quantitative polymerase chain reaction; spleen; two-dimensional gel electrophoresis
Abstract:
... This study aimed to unravel the regulatory roles of choline in activating immune responses and disease resistance of the orange-spotted grouper Epinephelus coioides. Fish were fed a choline-supplemented diet at 1 g kg−1 of feed for 30 days. Fish fed a fish meal basal diet without choline-supplement served as controls. At the end of the feeding trial, fish were challenged with Vibrio alginolyticus. ...
DOI:
10.1016/j.fsi.2017.04.022

19. Functional characterization and molecular modelling of FnFgBP, a surface protein from Streptococcus agalactiae

Author:
Shobana Ponnuvel; Dhanalakshmi Bandaru; Preethi Ragunathan; Karthe Ponnuraj
Source:
RSC advances 2016 v.6 no.94 pp. 91824-91835
ISSN:
2046-2069
Subject:
Gram-positive bacteria; Streptococcus agalactiae; Streptococcus pneumoniae; Streptococcus pyogenes; Western blotting; adhesins; binding properties; binding proteins; circular dichroism spectroscopy; enzyme-linked immunosorbent assay; fibrinogen; fibronectins; inclusion bodies; interferometry; ligands; prediction; sequence analysis; solubilization; surface proteins; urea; virulence
Abstract:
... Pneumococcal adherence and virulence factor A (PavA) were first identified in Streptococcus pneumoniae as an adhesin binding to fibronectin and studies suggested that it is an important virulence determinant. Homologs of PavA are found in many Gram-positive bacteria including S. agalactiae. However, to date, the details of its structure or mode of interaction with the host molecule(s) are not know ...
DOI:
10.1039/c6ra18275e

20. Laminin-111 enriched fibrin hydrogels for skeletal muscle regeneration

Author:
Madison Marcinczyk; Hady Elmashhady; Muhamed Talovic; Andrew Dunn; Faiz Bugis; Koyal Garg
Source:
Biomaterials 2017 v.141 pp. 233-242
ISSN:
0142-9612
Subject:
Western blotting; desmin; fibrin; fibrinogen; hydrogels; insulin-like growth factor I; interleukin-6; laminin; models; modulus of elasticity; muscle development; myoblasts; myogenin; skeletal muscle; vascular endothelial growth factors; viscoelasticity
Abstract:
... Laminin (LM)-111 supplementation has improved muscle regeneration in several models of disease and injury. This study investigated a novel hydrogel composed of fibrinogen and LM-111. Increasing LM-111 concentration (50–450 μg/mL) in fibrin hydrogels resulted in highly fibrous scaffolds with progressively thinner interlaced fibers. Rheological testing showed that all hydrogels had viscoelastic beha ...
DOI:
10.1016/j.biomaterials.2017.07.003