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1. Immunological heterogeneity of archaebacterial protein synthesis elongation factors Tu (EF‐Tu)

Author:
Orsola Tiboni; Rino Cella; Giuseppe Pasquale; Anna Maria Sanangelantoni; Piero Cammarano
Source:
FEMS microbiology letters 1988 v.51 no.2‐3 pp. 129-134
ISSN:
0378-1097
Subject:
Sulfolobus solfataricus; Western blotting; antigens; enzyme-linked immunosorbent assay; nucleotide sequences; phylogeny; polyclonal antibodies; protein synthesis; ribosomal RNA
Abstract:
... Polyclonal antibodies were raised against the EF‐Tu of the archaebacterium Sulfolobus solfataricus and cross‐reactivities of EF‐Tus of other, phylogenetically disparate archaebacteria were determined using Western blotting and ELISA. The results demonstrate a high degree of heterogeneity of archaebacterial Tu factors with recognition by S. solfataricus EF‐Tu antibodies ranging from 48% to ...
DOI:
10.1111/j.1574-6968.1988.tb02983.x

2. Serological potential for fungal identification

Author:
POLONELLI, LUCIANO; MORACE, GIULIA
Source:
Botanical journal of the Linnean Society 1989 v.99 no.1 pp. 33-38
ISSN:
0024-4074
Subject:
Candida; Western blotting; affinity chromatography; agglutination; animals; antigen detection; antiserum; cross reaction; epitopes; hybridomas; monoclonal antibodies; yeasts
Abstract:
... POLONELLI, L. & MORACE, G., 1989. Serological potential for fungal identification. Specific antigens are valuable for the identification of fungal cultures. Early attempts to immunoidentify fungi were hampered by heterogeneity of antigens, antibody preparations and use of improper serological procedures. In recent years, the double diffusion exoantigcn technique has proved to be the most effective ...
DOI:
10.1111/j.1095-8339.1989.tb00390.x

3. Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

Author:
Gary W. Black; Catherine M. Lyons; Edwin Williams; John Colby; Michael Kehoe; Catherine O'Reilly
Source:
FEMS microbiology letters 1990 v.70 no.3 pp. 249-254
ISSN:
0378-1097
Subject:
DNA libraries; Pseudomonas thermocarboxydovorans; Western blotting; blood serum; carbon monoxide; clones; genes; plasmids
Abstract:
... Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non‐identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti‐CODH serum. Western blotting experiments revealed a protein which co‐migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones iso ...
DOI:
10.1111/j.1574-6968.1990.tb14005.x

4. Immunohistochemical Identification of Infectious Pancreatic Necrosis Virus in Paraffin-Embedded Tissues of Atlantic Salmon (Salmo Salar)

Author:
Øystein Evensen; Espen Rimstad
Source:
Journal of veterinary diagnostic investigation v.2 no.4 pp. 288-293
ISSN:
1040-6387
Subject:
Infectious pancreatic necrosis virus; Salmo salar; Western blotting; antigens; antiserum; immunohistochemistry; neutralization tests; rabbits; serotypes
Abstract:
... Infectious pancreatic necrosis virus serotype Sp was identified by immunohistochemistry in formaldehyde-fixed and paraffin-embedded tissue of Atlantic salmon (Salmo salar). The immunoreaction was present in degenerating and necrotic cells in exocrine pancreatic cells. Cross reactions were observed with rabbit antisera against serotypes Sp, Ab, and VR-299 in neutralization tests and western blottin ...
DOI:
10.1177/104063879000200406

5. Immunological evidence for a ribulose bisphosphate carboxylase‐like protein in the symbiotic dinoflagellate Symbiodinium sp.

Author:
Holger Gerberding; Yvette McNeil; David Yellowlees; David J. Miller
Source:
FEMS microbiology letters 1990 v.70 no.3 pp. 343-346
ISSN:
0378-1097
Subject:
Symbiodinium; Western blotting; antibodies; beets; light intensity; ribulose; ribulose-bisphosphate carboxylase; sequence homology
Abstract:
... Whereas previously there has been no convincing evidence for ribulose bisphosphate carboxylase in dinoflagellates, a strong and highly specific reaction was observed when antibodies to the denatured large subunit of the (silver beet) protein were used to probe Western blots of whole soluble fractions of various Symbiodinium isolates. No reaction was observed using extracts from Symbiodinium isolat ...
DOI:
10.1111/j.1574-6968.1990.tb14001.x

6. Nitrogenase confined to randomly distributed trichomes in the marine cyanobacterium trichodesmium thiebautii1

Author:
Birgitta Bergman; Edward J. Carpenter
Source:
Journal of phycology 1991 v.27 no.2 pp. 158-165
ISSN:
0022-3646
Subject:
Trichodesmium thiebautii; Western blotting; antiserum; cytoplasm; molecular weight; nitrogen-fixing cyanobacteria; nitrogenase; transmission electron microscopy; trichomes
Abstract:
... Nitrogenase reductase (Fe‐protein) was detected in the marine planktonic cyanobacterium Trichodesmium. The molecular weight was about 38 kD, as shown by western blotting using anti ‐Rhodospirillum rubrum nitrogenase reductase antiserum. The enzyme was confined to a limited number (ca. 10–40%) of randomly distributed trichomes in the Trichodesmium colonies, as shown by immunogold localization ...
DOI:
10.1111/j.0022-3646.1991.00158.x

7. Monoclonal antibodies against Pseudomonas aeruginosa elastase: A neutralizing antibody which recognizes a conformational epitope related to an active site of elastase

Author:
Shin‐ichi YOKOTA; Hiroshi OHTSUKA; Hiroshi NOGUCHI
Source:
European journal of biochemistry 1992 v.206 no.2 pp. 587-593
ISSN:
0014-2956
Subject:
EDTA (chelating agent); Pseudomonas aeruginosa; Western blotting; active sites; chelating agents; elastase; elastin; enzyme activity; enzyme-linked immunosorbent assay; epitopes; fluorescein; hybridomas; metalloproteinases; mice; monoclonal antibodies; neutralization; neutralizing antibodies
Abstract:
... We have established seven murine hybridoma cell lines which produce monoclonal antibodies (mAbs) against Pseudomonas aeruginosa elastase. The seven mAbs recognized at least six different epitopes on the elastase molecule. All mAbs inhibited both enzymatic activities of elastase and protease, in which elastin fluorescein and hide powder azure were used as substrates, respectively. One of them, mAb ...
DOI:
10.1111/j.1432-1033.1992.tb16963.x

8. Purification and properties of prostaglandin 9‐ketoreductase from pig and human kidney: Identity with human carbonyl reductase

Author:
Ardrea SCHIEBER; Rainer W. FRANK; Sandro GHISLA
Source:
European journal of biochemistry 1992 v.206 no.2 pp. 491-502
ISSN:
0014-2956
Subject:
NADP (coenzyme); Western blotting; brain; carbonyl compounds; enzymes; humans; hydrogen; isoelectric focusing; kidneys; metabolites; molecular weight; peptides; placenta; polyacrylamide gel electrophoresis; polyclonal antibodies; prostaglandins; swine
Abstract:
... Prostaglandin 9‐ketoreductase (PG‐9‐KR) was purified from pig kidney to homogeneity, as judged by SDS/PAGE using an improved procedure. The enzyme is pro‐S stereoselective with regard to hydrogen transfer from NADPH with prostaglandin E2 as substrate and reduces its 9‐keto group with approximately 90% stereoselectivity to form prostaglandin F2α. Approximately 8% of the prostaglandin F f ...
DOI:
10.1111/j.1432-1033.1992.tb16952.x

9. Molecular characterization of a 70 kDa heat‐shock protein of bean mitochondria

Author:
Vincent Vidal; Benoit Ranty; Marietta Dillenschneider; Martine Charpenteau; Raoul Ranjeva
Source:
plant journal 1993 v.3 no.1 pp. 143-150
ISSN:
0960-7412
Subject:
Western blotting; antibodies; beans; complementary DNA; electrophoresis; heat-shock protein 70; mitochondria; open reading frames; polypeptides; protein aggregates; sequence analysis; threonine; transcription (genetics); translation (genetics)
Abstract:
... A bean cDNA clone that specifies a 70 kDa heat‐shock protein (hsp70) has been isolated and sequenced. The nucleotide sequence analysis shows that the cDNA could encode a 72 kDa protein that is highly related to prokaryotic and mitochondrial members of the hsp70 family. The predicted protein was found to contain an amino‐terminal extension typical of transit sequences. The in vitro transcription/tr ...
DOI:
10.1046/j.1365-313X.1993.t01-6-00999.x

10. Pathogenesis‐related proteins in ozone‐exposed Norway spruce [Picea abies (Karst) L.]

Author:
SIRPA O. KÄRENLAMPI; KIRSTI AIRAKSINEN; ANU T. E. MIETTINEN; HARRI I. KOKKO; JARMO K. HOLOPAINEN; LAURI V. KÄRENLAMPI; REIJO O. KARJALAINEN
Source:
new phytologist 1994 v.126 no.1 pp. 81-89
ISSN:
1469-8137
Subject:
Picea abies; Western blotting; antibodies; biomarkers; corn; ozone; pathogenesis; pathogenesis-related proteins; pathogens; tobacco; trees
Abstract:
... In a search for biochemical markers indicating the impact of ozone on plants. Pathogenesis related (PR) proteins were studied. The following observations were made: (1) PR‐proteins are present in gymnosperms, exemplified by Norway spruce (Picea abies) and (2) ozone induces PR‐proteins in Norway spruce. Studies were made by Western blot analysis using antibodies raised against seven PR‐proteins; fi ...
DOI:
10.1111/j.1469-8137.1994.tb07532.x

11. Serological Detection of Members of the Potyviridae with Polyclonal Antisera

Author:
J. Richter; E. Proll; F. Rabenstein; A. Stanarius
Source:
Phytopathologische Zeitschrift 1994 v.142 no.1 pp. 11-18
ISSN:
0931-1785
Subject:
Barley mild mosaic virus; Ryegrass mosaic virus; Turnip mosaic virus; Western blotting; antigens; antiserum; cross reaction; enzyme-linked immunosorbent assay; particles; plums; potatoes; proteins; viruses
Abstract:
... The cross‐reactivity of two polyclonal antisera with various members of the Potyviridae was determined using four different serological methods. An antiserum (no. 314) prepared against apparently intact turnip mosaic potyvirus (TuMV) particles showed a high cross‐reactivity with all of 20 aphid‐borne potyviruses tested as well as the mite‐borne ryegrass mosaic rymovirus (RGMV) and the fungus‐borne ...
DOI:
10.1111/j.1439-0434.1994.tb00002.x

12. Use of Polyclonal Antibodies to Identify Mycoplasma‐like organisms (MLOs) From the Sudan and from Thailand

Author:
E. Saeed; N. Sarindu; D. L. Davies; M. F. Clark; J. Roux; M. T. Cousin
Source:
Phytopathologische Zeitschrift 1994 v.142 no.4 pp. 345-349
ISSN:
0931-1785
Subject:
Catharanthus roseus; Crotalaria juncea; Phytoplasma; Sesamum indicum; Western blotting; antigens; crops; enzyme-linked immunosorbent assay; extracts; faba beans; molecular weight; polyacrylamide gel electrophoresis; polyclonal antibodies; soybeans; testing; witches' broom; Sudan; Thailand
Abstract:
... Rabbit polyclonal antibodies prepared against faba bean phyllody MLO from the Sudan reacted with its homologous antigen and with extracts of Catharanthus roseus experimentally infected with the same or a related MLO from Crotalaria saltiana showing symptoms of phyllody disease, as well as with extracts of naturally MLO‐infected C. saltiana growing in the field in the Sudan. The antibodies also rea ...
DOI:
10.1111/j.1439-0434.1994.tb00032.x

13. Antibodies to Canine Granulocyte Colony-stimulating Factor Induce Persistent Neutropenia

Author:
W. J. Reagan; D. Murphy; M. Battaglino; P. Bonney; T. C. Boone
Source:
Veterinary pathology v.32 no.4 pp. 374-378
ISSN:
1544-2217
Subject:
Western blotting; animal pathology; antibodies; antiserum; bone marrow; dogs; granulocyte colony-stimulating factor; hyperplasia; necropsy; neutropenia; neutrophils; rabbits
Abstract:
... A severe persistent neutropenia developed in a rabbit that was injected intradermally with 120, 60, 60, and 120 μg of recombinant canine granulocyte colony-stimulating factor (cG-CSF) on days 1, 22, 31, and 44, respectively. The neutropenia was present from day 44 to day 205. The nadir of the neutropenia (60 cells/μl) occurred in conjunction with peak antibody titer (640,000) to cG-CSF on day 58. ...
DOI:
10.1177/030098589503200405

14. Degradation of antenna chlorophyll-binding protein CP43 during photoinhibition of photosystem II

Author:
Yamamoto, Y.; Akasaka, T.
Source:
Biochemistry 1995 v.34 no.28 pp. 9038-9045
ISSN:
0006-2960
Subject:
D1 protein; Western blotting; aerobic conditions; antibodies; crosslinking; gels; hydroxylamine; molecular weight; pH; photoinhibition; photosystem II; polyacrylamide gel electrophoresis; proteins; sodium dodecyl sulfate; spinach; urea; white light
Abstract:
... When photosystem II (PS II) membranes from spinach were treated with Tris (0.8 M, pH 9.0) and illuminated with white light (5000 microeinsteins m(-2) s(-1) under aerobic conditions at 25 degrees C, not only were the reaction center-forming D1 and D2 proteins degraded but the antenna chlorophyll-binding protein CP43 was also degraded. Three products of the degradation of CP43, with molecular masses ...
DOI:
10.1021/bi00028a012
PubMed:
7619803

15. An independent role of cytochrome c-550 in cyanobacterial photosystem II as revealed by double-deletion mutagenesis of the psbO and psbV genes in Synechocystis sp. PCC 6803

Author:
Shen, J.R.; Burnap, R.L.; Inoue, Y.
Source:
Biochemistry 1995 v.34 no.39 pp. 12661-12668
ISSN:
0006-2960
Subject:
Synechocystis sp. PCC 6803; Western blotting; genes; lighting; mutagenesis; mutants; oxygen production; phenotype; photoinhibition; photosystem II; thermoluminescence
Abstract:
... Cytochrome (cyt) c-550 and the 33 kDa protein are two extrinsic components that function in maintaining oxygen evolution in cyanobacterial cells. Deletion of either of the two components has been shown to result in cyanobacterial phenotypes that are still capable of photoautotrophic growth albeit with a reduced rate. In order to study the function of cyt c-550 in cyanobacterial photosystem II (PSI ...
DOI:
10.1021/bi00039a023
PubMed:
7548017

16. Biochemical and immunological markers for the discrimination of three Tilapia species: T. zillii Gervais T. guineensis Bleeker and T. dageti Thys v.d. Audenaerde (Pisces: Cichlidae) from West Africa

Author:
Oberst, S.; Abban, E.K.; Villwock, W.
Source:
Aquaculture research 1996 v.27 no.4 pp. 235-244
ISSN:
1355-557X
Subject:
Cichlidae; Western blotting; agglutination; agglutination tests; alleles; biomarkers; erythrocytes; immunoassays; immunoelectrophoresis; loci; muscle protein; polyacrylamide gel electrophoresis; screening; soybeans; taxonomy; Ghana
Abstract:
... General muscle proteins and parvalbumins of Tilapia dageti (Thys van den Audenaerde), T. guineensis (Bleeker) and T. zillii (Gervais) (Pisces, Cichlidae) which occur sympatrically in Ghana were subjected to various electrophoretic and immunological assays, and erythrocytes of the species underwent agglutination tests. These investigations aimed to identify species-specific markers, as discriminati ...
DOI:
10.1111/j.1365-2109.1996.tb00990.x

17. A Candida maltosa mutant defective in alanine aminotransferase: isolation and L-alanine assimilation

Author:
Umemura, I.; Yanagiya, K.; Komatsubara, S.; Sato, T.; Tosa, T.
Source:
Applied microbiology and biotechnology 1996 v.45 no.4 pp. 519-524
ISSN:
0175-7598
Subject:
Candida maltosa; Western blotting; alanine; alanine transaminase; antibodies; carbon; glutamate dehydrogenase; mutants; nitrogen; pyruvic acid
Abstract:
... Candida maltosa JCM1504 can grow well on L-alanine as a sole carbon and nitrogen source. We found that the activities of alanine aminotransferase (AlaAT) and NAD-dependent glutamate dehydrogenase were remarkably induced when glucose-grown cells were transferred to medium containing L-alanine. This suggested that C. maltosa has an inducible L-alanine degradation system including the above two enzym ...
DOI:
10.1007/s002530050722

18. Cloning of the fliI gene from Rhodobacter sphaeroides WS8 by analysis of a transposon mutant with impaired motility

Author:
Ian Gordon Goodfellow; Charles Edward Pollitt; Renee Elizabeth Sockett
Source:
FEMS microbiology letters 1996 v.142 no.1 pp. 111-116
ISSN:
0378-1097
Subject:
DNA; Rhodobacter sphaeroides; Western blotting; bacterial motility; drug resistance; flagellin; genes; mutants; phenotype; restriction mapping; sequence analysis; transposons
Abstract:
... A transposon mutant of Rhodobacter sphaeroides WS8 was isolated that showed reduced swarming on soft agar plates. Liquid cultures of this mutant (M18) showed a low percentage of motile swimming cells in mid‐exponential phase and a low level of extracellular flagellin protein by Western blotting. M18 was complemented by a clone from a library of R. sphaeroides WS8 DNA, and restriction mapping of th ...
DOI:
10.1111/j.1574-6968.1996.tb08416.x

19. Comparison of ELISA and immunoblotting techniques for the detection of cherry mottle leaf virus

Author:
James, D.; Mukerji, S.
Source:
Annals of applied biology 1996 v.129 no.1 pp. 13-23
ISSN:
0003-4746
Subject:
plant viruses; Prunus avium; polyclonal antibodies; Prunus tomentosa; monoclonal antibodies; enzyme-linked immunosorbent assay; Western blotting; detection
DOI:
10.1111/j.1744-7348.1996.tb05727.x

20. A Dot Immunobinding Assay (Dot-ELISA) for the Rapid Serodiagnosis of Salmonella Enteritidis Infection in Chickens

Author:
Samuel D. Charles; Srinand Sreevatsan; Russell F. Bey; V. Sivanandan; D. A. Halvorson; Kakambi V. Nagaraja
Source:
Journal of veterinary diagnostic investigation v.8 no.3 pp. 310-314
ISSN:
1040-6387
Subject:
Salmonella Enteritidis; Western blotting; antibodies; antigens; blood serum; chickens; flocks; molecular weight; monitoring; outer membrane proteins; polypeptides; poultry diseases; screening; serodiagnosis
Abstract:
... A dot immunobinding assay (DIA) was developed for the detection of antibodies to Salmonella enteritidis. Western blot analysis of outer membrane proteins from SE identified 2 polypeptides of molecular masses 43 and 46 kD that were specific for S. enteritidis. These 2 polypeptides were utilized as antigens in the DIA. The DIA was tested on sera from chickens experimentally infected with S. enteriti ...
DOI:
10.1177/104063879600800306

21. Enrichment of yeast protein tyrosine kinase activity by substrate affinity chromatography

Author:
Adamíková, L'ubica; Resnick, Ross J.; Tomáška, L'Ubomír
Source:
Yeast 1996 v.12 no.9 pp. 833-838
ISSN:
0749-503X
Subject:
Saccharomyces cerevisiae; Western blotting; affinity chromatography; cell fractionation; enzyme activity; glutamic acid; protein-tyrosine kinases; tyrosine; yeasts
Abstract:
... The direct biochemical analysis of protein tyrosine kinases from yeast has been difficult due to their very low activity in crude cell lysates. Here we present a procedure for the enrichment and partial purification of protein tyrosine kinases from Saccharomyces cerevisiae based on single‐step substrate affinity chromatography using a synthetic random co‐polymer of glutamic acid and tyrosine. Frac ...
DOI:
10.1002/(SICI)1097-0061(199607)12:9<833::AID-YEA977>3.0.CO;2-C
PubMed:
8840500

22. Evaluation of an enzyme‐linked immunosorbent assay (ELISA) for the serological diagnosis of canine sarcoptic mange

Author:
SET BORNSTEIN; PER THEBO; GÓRAN ZAKRISSON
Source:
Veterinary dermatology 1996 v.7 no.1 pp. 21-28
ISSN:
0959-4493
Subject:
Cheyletiella; Ctenocephalides canis; Demodex canis; Linognathus setosus; Otodectes cynotis; Sarcoptes scabiei; Western blotting; antigens; dogs; enzyme-linked immunosorbent assay; humans; isoelectric point; molecular weight; polyacrylamide gel electrophoresis; scabies; serodiagnosis; sodium sulfate; veterinary medicine
Abstract:
... The purpose of this study was to evaluate a serodiagnostic test (enzyme‐linked immunosorbent assay; ELISA) for sarcoptic mange in dogs and to characterize the assay antigen, based on the mite Sarcoptes scabiei var. vulpes. The ELISA, applied to sera from 359 dogs suspected of having sarcoptic mange, showed a sensitivity and specificity of 92 and 96%, respectively. Sodium dodecyl sulphate polyacryl ...
DOI:
10.1111/j.1365-3164.1996.tb00222.x

23. Identification substrate proteins for cathepsin L that are selectively hydrolyzed during the differentiation of imaginal discs of Sarcophaga peregrina

Author:
Homma, K.I.; Natori, S.
Source:
European journal of biochemistry 1996 v.240 no.2 pp. 443-447
ISSN:
0014-2956
Subject:
Sarcophaga peregrina; Western blotting; cathepsin L; developmental stages; embryogenesis; fluorescent antibody technique; imaginal discs; molecular weight; proteins
Abstract:
... Previously, we showed that cathepsin L is essential for the differentiation of imaginal discs of the flesh fly [Homma, K. & Natori, S. (1994) J. Biol. Chem. 269, 15258 - 15264]. We have now identified imaginal disc proteins that are susceptible to digestion by cathepsin L and showed that they are selectively hydrolyzed during imaginal disc differentiation. Two of these proteins, with molecular mas ...
DOI:
10.1111/j.1432-1033.1996.0443h.x

24. Immunoblot and Ultrastructural Analysis of Antigens Extracted from Dichelobacter Nodosus with Potassium Thiocyanate

Author:
Richard Whittington
Source:
Journal of veterinary diagnostic investigation v.8 no.3 pp. 315-323
ISSN:
1040-6387
Subject:
Dichelobacter nodosus; Western blotting; antigens; electron microscopy; enzyme-linked immunosorbent assay; false positive results; molecular weight; potassium thiocyanate; sheep
Abstract:
... The antigens extracted from Dichelobacter nodosus with potassium thiocyanate were analyzed by western blotting with sera from footrot-free sheep and from sheep infected with D. nodosus to identify components specific for infection. Several components with molecular mass < 33 kD were associated with infection, particularly bands of 32.5 kD, 30.5 kD, and 28 kD. Components with molecular mass > 56 kD ...
DOI:
10.1177/104063879600800307

25. Immunological characterization of serine‐glyoxylate aminotransferase and hydroxypyruvate reductase from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2

Author:
Tairo Hagishita; Toyokazu Yoshida; Yoshikazu Izumi; Toshio Mitsunaga
Source:
FEMS microbiology letters 1996 v.142 no.1 pp. 49-52
ISSN:
0378-1097
Subject:
Hyphomicrobium methylovorum; Methylobacterium; Western blotting; antibodies; bacteria; enzymes
Abstract:
... Immunological characterization of serine‐glyoxylate aminotransferase and hydroxypyruvate reductase, key enzymes for the assimilation of one‐carbon compounds in methylotrophs, was performed using antibodies raised against these enzymes purified from Hyphomicrobium methylovorum GM2. Immunodiffusion studies indicated that serine‐glyoxylate aminotransferase and hydroxypyruvate reductase of all seven H ...
DOI:
10.1111/j.1574-6968.1996.tb08406.x

26. Immunological detection of proteins similar to bacterial proteases in higher plant chloroplasts

Author:
Ostersetzer, O.; Tabak, S.; Yarden, O.; Shapira, R.; Adam, Z.
Source:
European journal of biochemistry 1996 v.236 no.3 pp. 932-936
ISSN:
0014-2956
Subject:
Escherichia coli; Hordeum vulgare; Oryza sativa; Pisum sativum; Western blotting; antibodies; barley; chloroplast genome; chloroplasts; etiolation; genes; leaf extracts; leaves; peas; protein degradation; protein synthesis; proteinases; proteins; proteolysis; rice; roots
Abstract:
... Despite numerous demonstrations of protein degradation in chloroplasts of higher plants, little is known about the identity of the proteases involved in these reactions. To identify chloroplast proteases by immunological means, we investigated two proteins: ClpP, a protein similar to the proteolytic subunit of the bacterial ATP-dependent Clp protease, for which a gene is found in the chloroplast g ...
DOI:
10.1111/j.1432-1033.1996.00932.x
PubMed:
8665915

27. Improvement of PrPSc-detection in mouse spleen early at the preclinical stage of scrapie with collagenase-completed tissue homogenization and Sarkosyl-NaCl extraction of PrPSc

Author:
Grathwohl, K.U.D.; Horiuchi, M.; Ishiguro, N.; Shinagawa, M.
Source:
Archives of virology 1996 v.141 no.10 pp. 1863-1874
ISSN:
0304-8608
Subject:
prions; diagnostic techniques; spleen; scrapie; Western blotting
Abstract:
... Scrapie in sheep has recently become again a target of control measures and eradication programs. Crucial for the effectiveness of these measures is the detection of infected sheep during the long and potentially hazardous incubation period. However, routine-diagnosis is mostly limited to clinical examinations when disease becomes apparent, and to postmortem investigations. Through the detection o ...
DOI:
10.1007/BF01718200
PubMed:
8920821

28. Peroxisomal and mitochondrial carnitine acetyltransferases of the n-alkane-assimilating yeast Candida tropicalis: analysis of gene structure and translocation products

Author:
Kawachi, H.; Atomi, H.; Ueda, M.; Tanaka, A.
Source:
European journal of biochemistry 1996 v.238 no.3 pp. 845-852
ISSN:
0014-2956
Subject:
Candida tropicalis; Saccharomyces cerevisiae; Western blotting; carnitine; carnitine acetyltransferase; complementary DNA; gene expression; genes; methionine; mitochondria; molecular cloning; molecular weight; open reading frames; peroxisomes; proteins; sequence analysis; signal peptide; translation (genetics); yeasts
Abstract:
... A genomic DNA clone encoding carnitine acetyltransferases (EC 2.3.1.7), localized in two subcellular organelles, peroxisomes and mitochondria of an n-alkane-assimilating yeast Candida tropicalis, was isolated from the yeast lambda EMBL library using a carnitine acetyltransferase cDNA probe. Nucleotide sequence analysis disclosed that the open reading frame was 1881 bp, corresponding to 627 amino a ...
DOI:
10.1111/j.1432-1033.1996.0845w.x

29. Purification and characterization of a high molecular weight histone deacetylase complex (HD2) of maize embryos

Author:
Brosch, G.; Lusser, A.; Goralik-Schramel, M.; Loidl, P.
Source:
Biochemistry 1996 v.35 no.49 pp. 15907-15914
ISSN:
0006-2960
Subject:
Western blotting; acetylation; acetyltransferases; amino acids; antiserum; chromatin; corn; gel chromatography; germination; histone deacetylase; histones; molecular weight; polyacrylamide gel electrophoresis; polyclonal antibodies; polypeptides; rabbits
Abstract:
... The dynamic state of core histone acetylation is maintained by histone acetyltransferases and deacetylases. In germinating maize embryos, four nuclear histone deacetylases can be distinguished. From a chromatin fraction prepared at 72 h after start of embryo germination, we have purified the nuclear histone deacetylase HD2 to homogeneity. Using a sequence of chromatographic steps, we achieved the ...
DOI:
10.1021/bi961294x
PubMed:
8961957

30. Structural role of calcium for the organization of the cellulosome of Clostridium thermocellum

Author:
Choi, S.K.; Ljungdahl, L.G.
Source:
Biochemistry 1996 v.35 no.15 pp. 4906-4910
ISSN:
0006-2960
Subject:
Clostridium thermocellum; EDTA (chelating agent); Western blotting; aerobic conditions; amino acid sequences; antibodies; asparagine; calcium; cellulosome; epitopes; models; peptides; polyacrylamide gel electrophoresis; protein subunits
Abstract:
... The cellulosome of Clostridium thermocellum is a multipolypeptide complex of structural and catalytic subunits. Several of the catalytic subunits have at the carboxyl end a conserved duplicated region (CDR) which interacts with internally repeated elements (IREs) of scaffolding subunits such as CipA. This interaction requires calcium. The two parts of the CDR region here designated CDR1 and CDR2 ( ...
DOI:
10.1021/bi9524631
PubMed:
8664282

31. A common pathway for p10 and calyx proteins in progessive stages of polyhedron envelope assembly in AcMNPV-infected Spodoptera frugiperda larvae

Author:
Lee, S.Y.; Poloumienko, A.; Belfry, S.; Qu, X.; Chen, W.; MacAfee, N.; Morin, B.; Lucarotti, C.; Krause, M.
Source:
Archives of virology 1996 v.141 no.7 pp. 1247-1258
ISSN:
0304-8608
Subject:
Baculoviridae; Western blotting; Spodoptera frugiperda; cell lines; Northern blotting
Abstract:
... The assembly of the polyhedron envelope in baculovirus-infected cells has been the subject of several studies, yet it is still poorly understood. We have used immunogold-labelled antibodies to two baculovirus proteins, p10 and calyx (also referred to as polyhedron envelope protein or PEP), to follow envelope assembly in AcMNPV-infected tissues of Spodoptera frugiperda larvae. We show that, in wild ...
DOI:
10.1007/BF01718828

32. The multiple forms of starch-branching enzyme I in Solanum tuberosum

Author:
Khoshnoodi, J.; Blennow, A.; Ek, B.; Rask, L.; Larsson, H.
Source:
European journal of biochemistry 1996 v.242 no.1 pp. 148-155
ISSN:
0014-2956
Subject:
1,4-alpha-glucan branching enzyme; Solanum tuberosum; Western blotting; alleles; amino acids; complementary DNA; cultivars; enzyme activity; leaf extracts; leaves; messenger RNA; molecular weight; nucleotide sequences; polymerase chain reaction; proteolysis; sequence analysis; steel; tubers
Abstract:
... Western blot analysis showed the presence of three forms of starch-branching enzyme (SBE), with apparent molecular masses of 103, 97 and 80 kDa, in extracts of leaves and stored tubers of Solanum tuberosum. The 80-kDa form was absent in extracts of fresh tuber. Active 80-kDa enzyme was partially purified from stored tubers and sequence analysis showed that it, similar to the two larger enzyme form ...
DOI:
10.1111/j.1432-1033.1996.0148r.x
PubMed:
8954164

33. Analysis of Low Temperature Inducible Mechanism of γ-Glutamyltranspeptidase of Escherichia coli K-12

Author:
Wataru Hashimoto; Hideyuki Suzuki; Kenji Yamamoto; Hidehiko Kumagai
Source:
Bioscience, biotechnology, and biochemistry 1997 v.61 no.1 pp. 34-39
ISSN:
1347-6947
Subject:
Escherichia coli K12; Western blotting; biotechnology; gamma-glutamyltransferase; genes; temperature
Abstract:
... Escherichia coli K-12 cultured at 20°C has more γ-glutamyltranspeptidase (GGT: EC 2.3.2.2) activity than that cultured at 37 or 42°C. On Western blot analysis, E. coli K-12 cells cultured at 20°C produced more GGT protein than those cultured at 37°C. mRNA of the GGT gene (ggt) in the cells was also measured and it was found that the level of ggt mRNA at 20°C was 10-fold higher than that at 37°C. W ...
DOI:
10.1271/bbb.61.34

34. Antigenicity of VP60 structural proteinof rabbit haemorrhagic disease virus

Author:
E. Viaplana; J. Plana; A. Villaverde
Source:
Archives of virology 1997 v.142 no.9 pp. 1843-1848
ISSN:
0304-8608
Subject:
B-lymphocytes; DNA-directed RNA polymerase; Escherichia coli; Rabbit hemorrhagic disease virus; Western blotting; animals; coat proteins; epitopes; genes; immune response; viruses
Abstract:
... Five overlapping segments of the VP60 capsid protein gene of rabbit haemorrhagic disease virus have been expressed in E. coli under the control of the T7 RNA polymerase. After purification, the antigenicity of these denatured protein segments has been studied by reactivity with sera from both naturally infected and vaccinated animals in Western blot analysis. The amino terminus segments of the pro ...
DOI:
10.1007/s007050050201
PubMed:
9672641

35. Biochemical properties of protease resistant prion proteinPrPsc in natural sheep scrapie

Author:
J. Y. Madec; A. Vanier; A. Dorier; J. Bernillon; P. Belli; T. Baron
Source:
Archives of virology 1997 v.142 no.8 pp. 1603-1612
ISSN:
0304-8608
Subject:
Creutzfeldt-Jakob Syndrome; PrPSc proteins; Western blotting; bovine spongiform encephalopathy; cows; flocks; goats; humans; microorganisms; neurodegenerative diseases; pH; pathogens; proteinases; rabbits; scrapie; sheep; urea
Abstract:
... Prions are infectious agents involved in neurodegenerative diseases, such as scrapie in sheep and goats, bovine spongiform encephalopathy (BSE) in cows and Creutzfeldt-Jakob disease (CJD) in humans. These pathogens are characterized by unusual properties, and, in particular, by their strong resistance to common procedures of disinfection used against conventional microorganisms. A major component ...
DOI:
10.1007/s007050050183
PubMed:
9672622

36. Chloroplast import of four carotenoid biosynthetic enzymes in vitro reveals differential fates prior to membrance binding and oligomeric assembly

Author:
Bonk, M.; Hoffmann, B.; Von Lintig, J.; Schledz, M.; Al-Babili, S.; Hobeika, E.; Kleinig, H.; Beyer, P.
Source:
European journal of biochemistry 1997 v.247 no.3 pp. 942-950
ISSN:
0014-2956
Subject:
Western blotting; adenosine triphosphate; autoradiography; enzyme activity; imports; lycopene; peas; physiological transport; phytoene synthase; proteins; thylakoids
Abstract:
... The precursor proteins of the carotenogenic enzymes geranylgeranyl diphosphate synthase, phytoene synthase, phytoene desaturase and lycopene cyclase were imported into isolated pea chloroplasts. Geranylgeranyl diphosphate synthase remained soluble in the stroma in a free form and phytoene synthase associated to thylakoid membranes upon import, both as expected. Surprisingly, phytoene desaturase an ...
DOI:
10.1111/j.1432-1033.1997.00942.x

37. Comparison of five tests for the detection of antibodies against chlamydial (enzootic) abortion of ewes

Author:
Jones, G.E.; Low, J.C.; Machell, J.; Armstrong, K.
Source:
Veterinary record 1997 v.141 no.7 pp. 164-168
ISSN:
0042-4900
Subject:
ewes; Chlamydia; Chlamydophila psittaci; antibody detection; complement fixation tests; diagnostic techniques; blood serum; flocks; Chlamydophila pecorum; enzyme-linked immunosorbent assay; Western blotting; disease diagnosis
DOI:
10.1136/vr.141.7.164
PubMed:
9290194

38. Cyclin B1 expression in meiotically competent and incompetent goat oocytes

Author:
Hue, I.; Dedieu, T.; Huneau, D.; Ruffini, S.; Gall, L.; Crozet, N.
Source:
Gamete research 1997 v.47 no.2 pp. 222-228
ISSN:
1040-452X
Subject:
DNA probes; Western blotting; antibodies; cattle; complementary DNA; cyclins; developmental stages; genes; goats; granulosa cells; in situ hybridization; meiosis; messenger RNA; oocytes; polymerase chain reaction; polypeptides; transcription (genetics)
Abstract:
... To start determining the nature of meiotic incompetence in goat oocytes, we have examined the expression of one of the potential pre-MPF subunits: the cyclin B1. We have been isolating a small DNA probe encoding the goat cyclin B1 box to analyze the expression of the cyclin B1 gene in competent and incompetent goat oocytes. This probe was easily obtained by polymerase chain-reaction (PCR) on rever ...
DOI:
10.1002/(SICI)1098-2795(199706)47:2<222::AID-MRD14>3.0.CO;2-1
PubMed:
9136125

39. Detection of Bovine Immunodeficiency Virus Antibodies in Cattle by Western Blot Assay with Recombinant Gag Protein

Author:
Shucheng Zhang; Wenzhi Xue; Charles Wood; Qi-Min Chen; Sanjay Kapil; Harish C. Minocha
Source:
Journal of veterinary diagnostic investigation v.9 no.4 pp. 347-351
ISSN:
1040-6387
Subject:
cattle; Bovine immunodeficiency virus; antibody detection; recombinant proteins; accuracy; polymerase chain reaction; Western blotting; disease diagnosis; Kansas
Abstract:
... A western blot assay using purified recombinant bovine immunodeficiency virus gag protein has been developed for detection of bovine immunodeficiency virus antibodies in bovine serum samples. The test was standardized with known bovine immunodeficiency virus positive and negative bovine serum samples and the monoclonal antibody to gag protein. Both naturally and experimentally infected cattle sera ...
DOI:
10.1177/104063879700900401
PubMed:
9376421

40. Electrophoretic Detection of Myeloperoxidase, Protease, Lactoferrin and Lysozyme in Buffalo Polymorphonuclear Granular Acid Extracts

Author:
S.C. Roy; V.K. Singh; T. More
Source:
Veterinary research communications 1997 v.21 no.5 pp. 325-334
ISSN:
0165-7380
Subject:
Western blotting; acetates; blood; buffaloes; enzyme activity; granules; lactoferrin; lysozyme; milk; myeloperoxidase; pH; peptides; polyacrylamide gel electrophoresis; proteinases; sodium acetate; urea; viability
Abstract:
... Polymorphonuclear (PMN) cells of more than 90% viability and 92% purity were isolated from the peripheral blood of buffaloes. The cationic proteins were extracted with 0.2 mol/L sodium acetate, pH 4.0 from the granules in the PMN and subjected to both non-denaturing and denaturing acid urea polyacrylamide gel electrophoresis (AUPAGE) for identification of myeloperoxidase (MPO), lysozyme, protease ...
DOI:
10.1023/A:1005808203623

41. Expression of c-fos and jun protooncogenes in ovine trophoblasts in relation to interferon-tau expression and early implantation process

Author:
Xavier, F.; Lagarrigue, S.; Guillomot, M.; Gaillard-Sanchez, I.
Source:
Gamete research 1997 v.46 no.2 pp. 127-137
ISSN:
1040-452X
Subject:
Western blotting; antibodies; epithelium; gel electrophoresis; immunohistochemistry; messenger RNA; nuclear proteins; oligonucleotides; pregnancy; proto-oncogenes; sheep; trophoblast
Abstract:
... Expression of c-fos and jun protoon-cogenes was analyzed in the ovine extraembryonic trophoblast from days 14-18 of gestation, using Northern and Western blotting and immunohistochemistry. This study was carried out in relation to the early implantation process and the expression of interferon-tau, which is secreted in large amounts for a few days before implantation. Our results demonstrated that ...
DOI:
10.1002/(SICI)1098-2795(199702)46:2<127::AID-MRD3>3.0.CO;2-S
PubMed:
9021744

42. Expression of equine morbillivirus (EMV) matrix and fusion proteins and their evaluation as diagnostic reagents

Author:
L.-F. Wang; A. R. Gould; P. W. Selleck
Source:
Archives of virology 1997 v.142 no.11 pp. 2269-2279
ISSN:
0304-8608
Subject:
rabbits; proteins; complementary DNA; neutralization tests; glutathione transferase; Western blotting; clones; enzyme-linked immunosorbent assay; Hendra henipavirus; horses; Escherichia coli
Abstract:
... Full-length cDNA clones coding for the matrix (M) and fusion (F) proteins of equine morbillivirus (EMV) were isolated by RT-PCR, and expressed in Escherichia coli using two different expression systems. Western blot analysis indicated that the M and F proteins, expressed either by itself or as fusion proteins with glutathione S-transferase (GST), were insoluble and degraded after expression. Analy ...
DOI:
10.1007/s007050050241
PubMed:
9672592

43. Foot-and-mouth disease virus-infected but not vaccinated cattle develop antibodies against recombinant 3AB1 nonstructural protein

Author:
E. Silberstein; G. Kaplan; O. Taboga; S. Duffy; E. Palma
Source:
Archives of virology 1997 v.142 no.4 pp. 795-805
ISSN:
0304-8608
Subject:
Escherichia coli; Western blotting; antibodies; antigens; cattle; enzyme-linked immunosorbent assay; foot-and-mouth disease; proteins; vaccines; viruses
Abstract:
... Foot-and-mouth disease (FMD) vaccines induce antibodies against structural and some nonstructural proteins present in vaccine preparations. To differentiate between FMDV-infected and vaccinated animals, we developed immunochemical assays capable of detecting antibodies against a FMDV nonstructural protein. Recombinant nonstructural 3AB1 protein was expressed in E. coli and in insect cells and used ...
DOI:
10.1007/s007050050119
PubMed:
9170505

44. Heat shock protein 80 of Neurospora crassa, a cytosolic molecular chaperone of the eukaryotic stress 90 family, interacts directly with heat shock protein 70

Author:
Freitag, D.G.; Ouimet, P.M.; Girvitz, T.L.; Kapoor, M.
Source:
Biochemistry 1997 v.36 no.33 pp. 10221-10229
ISSN:
0006-2960
Subject:
NAD (coenzyme); Neurospora crassa; Western blotting; adenosine triphosphate; crosslinking; fluorescence; heat-shock protein 70; polyacrylamide gel electrophoresis; polyclonal antibodies; precipitin tests; tryptophan; uridine triphosphate
Abstract:
... The subunit structure of Hsp80, the most abundant heat-shock protein of Neurospora crassa, was examined by chemical cross-linking of the purified protein in vitro. Resolution of glutaraldehyde-treated Hsp80 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE suggests that the native state of this protein is a tetramer; the relative proportion of cross-linked species, estimated by ...
DOI:
10.1021/bi963030g
PubMed:
9254620

45. Heme-regulated eukaryotic initiation factor 2α kinase—A molecular indicator of haemolytic anemia

Author:
S. Anand; J. K. Pal
Source:
Journal of biosciences 1997 v.22 no.3 pp. 287-298
ISSN:
0250-5991
Subject:
Northern blotting; Western blotting; blood sampling; drugs; enzyme activity; hematocrit; hemoglobin; hemolytic anemia; monoclonal antibodies; patients; protein kinases; protein synthesis; rabbits; reticulocyte count; reticulocytes
Abstract:
... The heme-regulated enkaryotic initiation factor-2α (eIF-2α) kinase, also called the heme-regulated inhibitor (HRI), is a key regulator of protein synthesis in mammalian reticulocyte. HRI is almost undetectable in blood samples of normal rabbits and it increases by 12–15-fold in the reticulocytes of anemic rabbits. In order to determine if such an increase in the quantity of HRI is gradual during a ...
DOI:
10.1007/BF02703232

46. Heterologous gene expression of bovine plasmin in Lactococcus lactis

Author:
Arnau, J.; Hjerl-Hansen, E.; Israelsen, H.
Source:
Applied microbiology and biotechnology 1997 v.48 no.3 pp. 331-338
ISSN:
0175-7598
Subject:
Lactococcus lactis; Western blotting; active sites; bacteria; cattle; gene expression; genes; heterologous gene expression; hybridization; plasmin; promoter regions; secretion; signal peptide
Abstract:
... Heterologous production of bovine plasmin was studied in the industrially relevant bacterium Lactococcus lactis. Two sets of lactococcal gene expression signals were coupled to the region of the plasmin gene coding for the serine protease domain. When the promoter region of the prtP gene was used, plasmin was detected mainly intracellularly in strain BPL25 by Western blot hybridization. The intrac ...
DOI:
10.1007/s002530051058
PubMed:
9352676

47. Human T-lymphotropic Virus Type II Infection inVietnamese Thalassemic Patients

Author:
M. T. Lin; B. T. Nguyen; T. V. Binh; T. V. Be; T. Y. Chiang; L. H. Tseng; Y. C. Yang; K. H. Lin; Y. -C. Chen
Source:
Archives of virology 1997 v.142 no.7 pp. 1429-1440
ISSN:
0304-8608
Subject:
Western blotting; agglutination tests; antibodies; blood transfusion; humans; patients; phylogeny; polymerase chain reaction; proviruses; terminal repeat sequences; Europe; North America; Vietnam
Abstract:
... Anti-human T-lymphotropic virus type I/II (HTLV-I/II) antibodies were screened by particle agglutination test in a total of 66 patients with thalassemia major who received multiple transfusion from paid donors at the Blood Transfusion Hematology Center of Ho Chi Minh City in South Vietnam. HTLV-II infection was confirmed in 6 patients (9.1%) by Western blot analysis and/or polymerase chain reactio ...
DOI:
10.1007/s007050050170
PubMed:
9267453

48. Identification of three distinct antigenic sites in parapoxviruses

Author:
C. -P. Czerny; R. Waldmann; T. Scheubeck
Source:
Archives of virology 1997 v.142 no.4 pp. 807-821
ISSN:
0304-8608
Subject:
Orf virus; Western blotting; cross reaction; electron microscopy; enzyme-linked immunosorbent assay; epitopes; immunoglobulin M; mice; monoclonal antibodies; pathogenicity; viruses
Abstract:
... Monoclonal antibodies (Mabs) were generated in BALB/c mice immunized with gradient-purified particles, envelopes and cores of intracellular mature orf virus D-1701. Three distinct antigenic sites were identified in this virus strain. Their topographical relationships was determined by pairwise epitope specificity studies in competition ELISAs. One MAb (class IgM) neutralized virus infectivity. Fou ...
DOI:
10.1007/s007050050120
PubMed:
9170506

49. Immediate-early protein BICP22 of bovine herpesvirus 1 trans-represses viral promoters of different kinetic classes and is itself regulated by BICP0 at transcriptional and posttranscriptional levels

Author:
R. Köppel; B. Vogt; M. Schwyzer
Source:
Archives of virology 1997 v.142 no.12 pp. 2447-2464
ISSN:
0304-8608
Subject:
transcription factors; Bovine alphaherpesvirus 1; virion; Western blotting; transcription (genetics); genes; virus replication; antiserum; fluorescent antibody technique
Abstract:
... Bovine herpesvirus 1 (BHV-1) encodes four immediate-early (IE) proteins. The transactivators BICP0 and BICP4 are key regulatory elements in viral replication, and circ is a myristylated virion component, whereas BICP22 – originating from a spliced 1.7-kb transcript synthesized with dual IE and late kinetics – has not yet been characterized as a protein. In this study, Western blot and immunofluore ...
DOI:
10.1007/s007050050254
PubMed:
9672606

50. Immunochemically distinct NADP-linked isocitrate dehydrogenase isozymes in mitochondria and peroxisomes of Candida tropicalis

Author:
Imajo, T.; Kawachi, H.; Atomi, H.; Sanuki, S.; Yamamoto, S.; Ueda, M.; Tanaka, A.
Source:
Archives of microbiology 1997 v.168 no.5 pp. 389-395
ISSN:
0302-8933
Subject:
Candida tropicalis; DNA; Saccharomyces cerevisiae; Western blotting; acetates; alkanes; conserved sequences; genes; glucose; isocitrate dehydrogenase; isozymes; messenger RNA; mitochondria; molecular weight; open reading frames; peroxisomes; polyclonal antibodies; screening; yeasts
Abstract:
... Although peroxisomal localization of NADP-linked isocitrate dehydrogenase (Idp) was first demonstrated in Candida tropicalis, the mitochondrial isozyme has not been found in this yeast. Here we report that the presence of mitochondrial Idp in the yeast was demonstrated by screening for its gene with a DNA probe containing conserved sequences of Idps from various organisms. The nucleotide sequence ...
DOI:
10.1007/s002030050513
PubMed:
9325427

51. Oryzasin as an aspartic occurring in rice seeds: purification, characterization, and application to milk clotting

Author:
Asakura, T.; Watanabe, H.; Abe, K.; Arai, S.
Source:
Journal of agricultural and food chemistry 1997 v.45 no.4 pp. 1070-1075
ISSN:
0021-8561
Subject:
EDTA (chelating agent); Western blotting; ammonium sulfate; anion exchange chromatography; chymosin; cysteine proteinases; fractionation; gel chromatography; hydrolysis; kappa-casein; milk clotting; pH; pepsin; polyacrylamide gel electrophoresis; proteinase inhibitors; rice; seeds; skim milk
Abstract:
... An aspartic proteinase in rice seeds (oryzasin) was purified by (NH4)2SO4 fractionation, DEAE-celluose anion exchange chromatography, Sephadex G-100 gel filtration, Mono Q anion exchange chromatography, and pepstatin-affinity chromatography. SDS-PAGE showed the affinity-purified enzyme to have two molecular forms, 57 and 53 kDa, together with their probable autolysates appearing as two small bands ...
DOI:
10.1021/jf960582x

52. Relative toxicity of native Chilean Bacillus thuringiensis strains against Scrobipalpuloides absoluta (Lepidoptera: Gelechiidae)

Author:
Theoduloz, C.; Roman, P.; Bravo, J.; Padilla, C.; Vasquez, C.; Meza-Zepeda, L.; Meza-Basso, L.
Source:
Journal of applied microbiology 1997 v.82 no.4 pp. 462-468
ISSN:
1364-5072
Subject:
Bacillus thuringiensis; Culex pipiens; Gelechiidae; Western blotting; bacterial insecticides; bioassays; crystal proteins; genes; insect pests; insecticidal properties; larvae; lethal dose 50; moths; polyacrylamide gel electrophoresis; polymerase chain reaction; Chile
Abstract:
... The larva of Scrobipalpuloides absoluta, a South American moth, is the most devastating insect pest of tomato production in Chile. The potential for using bacterial insecticides was studied analysing the relative toxicity of native Bacillus thuringiensis (BT) isolates belonging to the Chilean collection. The polymerase chain reaction (PCR) technique was used in order to facilitate the prescreening ...
DOI:
10.1046/j.1365-2672.1997.00137.x

53. Stimulation of type I DNA topoisomerase gene expression by pseudorabies virus

Author:
M.-L. Wong; T.-Y. Ho; J.-H. Huang; C.-Y. Hsiang; T.-J. Chang
Source:
Archives of virology 1997 v.142 no.10 pp. 2099-2105
ISSN:
0304-8608
Subject:
Suid alphaherpesvirus 1; regulatory proteins; Western blotting; gene expression; transcription (genetics); cytopathogenicity; genes; viruses; DNA topoisomerase
Abstract:
... Previous results from our laboratory have demonstrated that type I DNA topoisomerase activity is required for the replication and gene expression of pseudorabies virus (PRV). In the present report, we further analyzed the expression of topoisomerase I in PRV-infected cells, and the western blot result showed that the expression of topoisomerase I was increased after virus infection. The increase s ...
DOI:
10.1007/s007050050228
PubMed:
9413519

54. Stress proteins HSP60 and HSP70 in three species of amphipods exposed to cadmium, diazinon, dieldrin and fluoranthene

Author:
Werner, I.; Nagel, R.
Source:
Environmental toxicology and chemistry 1997 v.16 no.11 pp. 2393-2403
ISSN:
0730-7268
Subject:
Hyalella azteca; Western blotting; biomarkers; cadmium; densitometry; diazinon; dieldrin; freshwater; heavy metals; lethal concentration 50; polycyclic aromatic hydrocarbons; protein synthesis; proteins; sediments
Abstract:
... To investigate the use of stress proteins hsp60 and hsp70 as sublethal biomarkers for contaminant exposure in sediments, two infaunal (Ampelisca abdita, estuarine; Rhepoxynius abronius, marine) and one epifaunal (Hyalella azteca, freshwater) amphipod species were exposed for 24 h to solutions of the heavy metal cadmium, the pesticides diazinon and dieldrin, and the polycyclic aromatic hydrocarbon ...
DOI:
10.1897/1551-5028(1997)016<2393:SPHAHI>2.3.CO;2

55. Structure and regulation of ferredoxin-dependent glutamase synthase from Arabidopsis thaliana. Cloning of cDNA, expression in different tissues of wild-type and gltS mutant strains, and light induction

Author:
Suzuki, A.; Rothstein, S.
Source:
European journal of biochemistry 1997 v.243 no.3 pp. 708-718
ISSN:
0014-2956
Subject:
Arabidopsis thaliana; NAD (coenzyme); Western blotting; amino acid composition; amino acid sequences; complementary DNA; corn; ecotypes; etiolation; leaves; lighting; messenger RNA; mutants; nucleotide sequences; polypeptides; roots; shoots
Abstract:
... Ferredoxin (Fd)-dependent glutamate synthase is present in green leaves, etiolated leaves, shoots and roots of Arabidopsis thaliana (ecotype Columbia). In photosynthetic green leaves and shoots, Fd-dependent glutamate synthase accounts for more than 96% Of the total glutamate synthase activity in vitro with the remaining activity derived from an enzyme that uses NADH as the electron donor. In etio ...
DOI:
10.1111/j.1432-1033.1997.00708.x
PubMed:
9057836

56. The coat protein gene of grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants

Author:
K. -S. Ling; H. -Y. Zhu; H. Alvizo; J. S. Hu; R. F. Drong; J. L. Slightom; D. Gonsalves
Source:
Archives of virology 1997 v.142 no.6 pp. 1101-1116
ISSN:
0304-8608
Subject:
Nicotiana benthamiana; Western blotting; amino acid sequences; cDNA libraries; clones; complementary DNA; double-stranded RNA; gene expression; genes; molecular weight; monoclonal antibodies; open reading frames; plant viruses; transgenic plants
Abstract:
... A lambda ZAP II cDNA library was constructed by cloning cDNA prepared from a high molecular weight double-stranded RNA (dsRNA, ca. 18 kb) isolated from grapevine leafroll associated closterovirus-3 (GLRaV-3) infected tissues. This cDNA library was immuno-screened with GLRaV-3 coat protein specific polyclonal and monoclonal antibodies and three immuno-positive clones were identified. Analysis of nu ...
DOI:
10.1007/s007050050145
PubMed:
9229001

57. Antibody response detected by immunoblot in respiratory tract washings of chickens after infection with Mycoplasma gallisepticum

Author:
Ellakany, H.; Fabian, K.; Nemeth, I.; Stipkovits, L.
Source:
Avian pathology 1998 v.27 no.6 pp. 547-554
ISSN:
0307-9457
Subject:
chickens; Mycoplasma gallisepticum; polypeptides; immune response; immunoglobulin A; immunoglobulin M; immunoglobulin G; blood serum; trachea (vertebrates); Western blotting
Abstract:
... Two experiments were conducted to test the sensitivity of Western blotting for detection of M. gallisepticum antibodies in respiratory washings and sera of infected chickens by mouse monoclonal antibodies to chicken IgG, IgM and IgA. In the first experiment, birds infected at 10 days of age were examined 2 weeks later. In the respiratory washings, IgA antibodies reacted with eight polypeptides of ...
DOI:
10.1080/03079459808419382
PubMed:
18484042

58. Calf thymus high mobility group proteins are nonenzymatically glycated but not significantly glycosylated

Author:
Lillian Medina; Robert S. Haltiwanger
Source:
Glycobiology 1998 v.8 no.2 pp. 191-198
ISSN:
1460-2423
Subject:
Western blotting; anion exchange chromatography; antibodies; calves; carbohydrates; glycation; glycosylation; lysine; nucleosomes; pH; peptides; staining; thymus gland
Abstract:
... Over the past decade, there have been many reports suggesting the presence of complex carbohydrates on nuclear and cytoplasmic proteins in mammalian cells. Some of the most often cited of these reports deal with the glycosylation of the high mobility group (HMG) proteins. These are relatively abundant chromosomal proteins that are known to be associated with nucleosomes and actively transcribed re ...
DOI:
10.1093/glycob/8.2.191

59. Characterisation of G serotype dependent non-antibody inhibitors of rotavirus in normal mouse serum

Author:
B. Beisner; D. Kool; A. Marich; I. H. Holmes
Source:
Archives of virology 1998 v.143 no.7 pp. 1277-1294
ISSN:
0304-8608
Subject:
Rotavirus; Vibrio cholerae; Western blotting; cattle; humans; mice; neutralization; polysaccharides; proteins; serotypes; sialidase; viruses; United Kingdom
Abstract:
... Serotype specific (non-immunoglobulin) inhibitors of rotavirus have been identified in normal mouse serum obtained from BALB/c, CBA, and BL10 mice. Sialic acid was essential for the neutralising activity as sera treated with the neuraminidase from Vibrio cholerae failed to neutralise rotavirus. G serotypes 4, 5, 7, 8, 9, & 10 were unaffected by the inhibitor(s) while G serotypes 1, 2, 6 and two G3 ...
DOI:
10.1007/s007050050375
PubMed:
9722874

60. Characterization of a Mutant of Lactococcus lactis with Reduced Membrane-bound ATPase Activity under Acidic Conditions

Author:
Seigo AMACHI; Kohei ISHIKAWA; Shuji TOYODA; Yasuo KAGAWA; Atsushi YOKOTA; Fusao TOMITA
Source:
Bioscience, biotechnology, and biochemistry 1998 v.62 no.8 pp. 1574-1580
ISSN:
1347-6947
Subject:
Lactococcus lactis subsp. lactis; Western blotting; adenosinetriphosphatase; biotechnology; gene expression; mutants; pH; structural genes; transcription (genetics); viability
Abstract:
... A mutant of Lactococcus lactis subsp. lactis C2 with reduced membrane-bound ATPase activity was characterized to clarify its acid sensitivity. The cytoplasmic pH of the mutant was measured in reference to the parental strain under various pH conditions. At low pH, the mutant could not maintain its cytoplasmic pH near neutral, and lost its viability faster than the parental strain. The ATPase activ ...
DOI:
10.1271/bbb.62.1574

61. Cloning and functional expression of AtCOQ3, the Arabidopsis homologue of the yeast COQ3 gene, encoding a methyltransferase from plant mitochondria involved in ubiquinone biosynthesis

Author:
Marie‐Hélène Avelange‐Macherel; Jacques Joyard
Source:
plant journal 1998 v.14 no.2 pp. 203-213
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; biosynthesis; methionine S-methyltransferase; complementary DNA; gene overexpression; Salmonella Typhimurium; yeasts; pea protein; Saccharomyces cerevisiae; Western blotting; amino acid sequences; amino acids; genes; cDNA libraries; mitochondria; mutants; molecular weight; Escherichia coli
Abstract:
... A mutant of Saccharomyces cerevisiae deleted for the COQ3 gene was constructed. COQ3 encodes a 3,4‐dihydroxy‐5‐hexaprenylbenzoate (DHHB) methyltransferase that catalyses the fourth step in the biosynthesis of ubiquinone from p‐hydroxybenzoic acid. A full length cDNA encoding a homologue of DHHB‐methyltransferase was cloned from an Arabidopsis thaliana cDNA library by functional complementation of ...
DOI:
10.1046/j.1365-313X.1998.00109.x

62. A DNA clone encoding the full-length infectious genome of odontoglossum ringspot tobamovirus and mutagenesis of its coat protein gene

Author:
H. H. Yu; S. M. Wong
Source:
Archives of virology 1998 v.143 no.1 pp. 163-171
ISSN:
0304-8608
Subject:
phenotype; electron microscopy; DNA; Nicotiana benthamiana; Oncidium; Odontoglossum ringspot virus; mutagenesis; Western blotting; enzyme-linked immunosorbent assay; pathogenicity; seedlings; mutants; start codon; RNA; Northern blotting; DNA-directed RNA polymerase
Abstract:
... A full-length DNA clone encoding the genome of odontoglossum ringspot tobamovirus (ORSV) was synthesized and placed adjacent to a bacteriophage T7 RNA polymerase promoter. Capped-RNA transcripts produced in vitro were highly infectious when mechanically inoculated onto seedlings of Nicotiana benthamiana and Oncidium Gower Ramsey. A representative clone, designated pOT2, caused a disease phenotype ...
DOI:
10.1007/s007050050276
PubMed:
9505974

63. Detection of bovine immunodeficiency-like virus infection in experimentally infected calves

Author:
T. Baron; D. Betemps; F. Mallet; V. Cheynet; D. Levy; P. Belli
Source:
Archives of virology 1998 v.143 no.1 pp. 181-189
ISSN:
0304-8608
Subject:
Escherichia coli; Western blotting; affinity chromatography; calves; enzyme-linked immunosorbent assay; genome; mononuclear leukocytes; polymerase chain reaction; viruses
Abstract:
... Detection of BIV virus infection by serological means, PCR and virus isolation in experimentally infected calves is described. Viral sequences were specifically detected by PCR in peripheral blood mononuclear cells (PBMCs), with primer systems located in the gag, pol and tat regions of the viral genome. An enzyme-linked oligosorbent assay (ELOSA) in microtiter plates is described, for the detectio ...
DOI:
10.1007/s007050050278
PubMed:
9505976

64. Effect of IS900 Gene of Mycobacterium paratuberculosis on Mycobacterium smegmatis

Author:
Saleh A. Naser; Robert F. Gillespie; Najih A. Naser; Fouad A.K. El-Zaatari
Source:
Current microbiology 1998 v.37 no.6 pp. 373-379
ISSN:
0343-8651
Subject:
Escherichia coli; Mycobacterium avium subsp. paratuberculosis; Mycobacterium smegmatis; Western blotting; antibodies; genes; open reading frames; plasmids
Abstract:
... Mycobacterium paratuberculosis is mycobactin dependent and contains multiple copies of the IS900 gene that encodes for p43 (46.5K protein). The correlation between the two characteristics has been investigated. A 3.2-kb BamHI fragment from M. paratuberculosis containing the 1.451 kb IS900 gene was cloned in Escherichia coli and Mycobacterium smegmatis with pcDNA II and pNEZ6.3 plasmids, respective ...
DOI:
10.1007/s002849900396
PubMed:
9806974

65. Effects of Botrytis cinerea infection on the must protein electrophoretic characteristics

Author:
Marchal, R.; Berthier, L.; Legendre, L.; Marchal-Delahaut, L.; Jeandet, P.; Maujean, A.
Source:
Journal of agricultural and food chemistry 1998 v.46 no.12 pp. 4945-4949
ISSN:
0021-8561
Subject:
infection; Botrytis cinerea; grape must; plant proteins; detection; polyacrylamide gel electrophoresis; Western blotting
Abstract:
... Qualitative effects of Botrytis cinerea infection on a must protein fraction were studied by comparing the electrophoretic patterns of musts obtained from healthy grapes or from grapes highly infected by B. cinerea. Evidence was obtained that proteins secreted by B. cinerea can degrade grape proteins. Most of the proteins present in the healthy must, between 20 and 30 kDa and a major glycoprotein ...
DOI:
10.1021/jf980453b

66. Evidence That a β-1,4-Endoglucanase Secreted by Acetobacter xylinum Plays an Essential Role for the Formation of Cellulose Fiber

Author:
Hyun Min KOO; Sung Hee SONG; Yu Ryang PYUN; Yu Sam KIM
Source:
Bioscience, biotechnology, and biochemistry 1998 v.62 no.11 pp. 2257-2259
ISSN:
1347-6947
Subject:
Gluconacetobacter xylinus; Western blotting; antibodies; biotechnology; cellulose; cellulosic fibers; culture media; enzymes
Abstract:
... Cellulose-producing Acetobacter xylinum has been known to secrete a cellulose-hydrolyzing β-1,4-endoglucanase (CMCax). When antibodies to recombinant CMCax were added to the culture medium, the formation of cellulose fiber was severely inhibited. Western blot analysis using the antibody showed that this enzyme was secreted into the medium even by a cellulose non-producing strain (Cel⁻). These resu ...
DOI:
10.1271/bbb.62.2257

67. Expression of Leˣ antigen in Schistosoma japonicum and S.haematobium and immune responses to Leˣ in infected animals: Lack of Leˣ expression in other trematodes and nematodes

Author:
A. Kwame Nyame; Russell Debose-Boyd; Troy D. Long; Victor C.W. Tsang; Richard D. Cummings
Source:
Glycobiology 1998 v.8 no.6 pp. 615-624
ISSN:
1460-2423
Subject:
Caenorhabditis elegans; Dirofilaria immitis; Fasciola hepatica; Haemonchus contortus; Lotus tetragonolobus; Schistosoma japonicum; Schistosoma mansoni; Western blotting; Wisteria floribunda; adults; agglutinins; antigens; autoimmunity; detergents; dogs; enzyme-linked immunosorbent assay; free-living nematodes; glycoproteins; heartworms; humans; immune response; intestines; molecular weight; monoclonal antibodies; polysaccharides; rodents; ruminants
Abstract:
... Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Leˣ) Galβ1→4(Fucα1→3)GlcNAcβ1→R, but not sialyl Lewis x (sLeˣ), antigen. We now report on our analyses of Leˣ and sLeˣ expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that ...
DOI:
10.1093/glycob/8.6.615

68. Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris

Author:
Paul François Gallet; Hélène Vaujour; Jean-Michel Petit; Abderrahman Maftah; Ahmad Oulmouden; Rafael Oriol; Christine Le Narvor; Michel Guilloton; Raymond Julien
Source:
Glycobiology 1998 v.8 no.9 pp. 919-925
ISSN:
1460-2423
Subject:
Golgi apparatus; Komagataella pastoris; Saccharomyces cerevisiae; Western blotting; amino acids; catalysts; culture media; freezing; heterologous gene expression; humans; signal peptide; substrate specificity; thawing; transferases; yeasts
Abstract:
... A stable GS115 Pichia pastoris recombinant strain was constructed to secrete a truncated form of the human α(1,3/4) fucosyltransferase (amino acids 45–361). Enzyme production resulted from a secretory pathway based on the pre-pro- α mating factor signal sequence of the yeast Saccharomyces cerevisiae. Following its transit through the Golgi apparatus, the enzyme accumulated in the periplasmic space ...
DOI:
10.1093/glycob/8.9.919

69. Induction of thaumatin‐like proteins (TLPs) in Rhizoctonia solani‐infected rice and characterization of two new cDNA clones

Author:
Rethinasamy Velazhahan; Kunwei Chen‐Cole; Coimbatore S. Anuratha; Subbaratnam Muthukrishnan
Source:
Physiologia plantarum 1998 v.102 no.1 pp. 21-28
ISSN:
0031-9317
Subject:
Northern blotting; Pseudomonas syringae pv. syringae; RNA; Rhizoctonia; Southern blotting; Thanatephorus cucumeris; Western blotting; antibodies; bacteria; fungi; leaf blight; rice; sequence analysis
Abstract:
... Thaumatin‐like proteins (TLPs) were shown to be induced in rice plants (cv. IR58) that were infected with the sheath blight fungus, Rhizoctonia solani. Western blot analysis revealed the presence of two TLPs with sizes of 25 and 24 kDa which are different from a previously reported TLP with a size of 15.6 kDa from rice plants infiltrated with the non‐pathogenic bacterium, Pseudomonas syringae pv. ...
DOI:
10.1034/j.1399-3054.1998.1020104.x

70. Isolation of a cytopathogenic virus from a case of porcine reproductive and respiratory syndrome (PRRS) and its characterization as parainfluenza virus type 2

Author:
E. Heinen; W. Herbst; N. Schmeer
Source:
Archives of virology 1998 v.143 no.11 pp. 2233-2239
ISSN:
0304-8608
Subject:
porcine reproductive and respiratory syndrome; Mammalian orthorubulavirus 5; virion; Bovine orthopneumovirus; breeding; nucleocapsid; Western blotting; Avian orthoavulavirus 1; fetus; viruses; sows; RNA
Abstract:
... From a lung of a fetus of a breeding sow showing PRRS-like symptoms a viral agent could be isolated. It was characterized as an enveloped, hemag-glutinating RNA virus. Ultrastructural examination of purified virus revealed paramyxovirus-like pleomorphic virions of approx. 200 nm in diameter. The helical nucleocapsids were about 18 nm in diameter. The virus was found to be antigenically related to ...
DOI:
10.1007/s007050050454
PubMed:
9856104

71. Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from Stereum purpureum

Author:
Yuji HASUI; Yohko FUKUI; Junko KIKUCHI; Nozomu KATO; Kazuo MIYAIRI; Toshikatsu OKUNO
Source:
Bioscience, biotechnology, and biochemistry 1998 v.62 no.5 pp. 852-857
ISSN:
1347-6947
Subject:
Chondrostereum purpureum; Western blotting; apples; biotechnology; culture filtrates; culture media; fungi; mannose; molecular weight; pH; polygalacturonase; substrate specificity
Abstract:
... Three endopolygalacturonases (endoPG Ia, Ib, and Ic) were isolated from the culture filtrate of Stereum purpureum, the causative fungus of apple silver-leaf disease. Their properties, including specific activities, optimum pHs, thermal stabilities, and kinetic parameters (K ₘ and V ₘₐₓ) were compared. Their properties were very similar to one another except for the substrate specificity and relati ...
DOI:
10.1271/bbb.62.852

72. Low Sucrase Activity in the Small Intestine of a Senescence-accelerated Strain of Mouse, SAMP1

Author:
Makoto TAKENOSHITA; Mitsuharu YABUNE; Hiromi KATSURA; Ryoichi YAMAJI; Hiroshi INUI; Kazutaka MIYATAKE; Yoshihisa NAKANO
Source:
Bioscience, biotechnology, and biochemistry 1998 v.62 no.5 pp. 965-969
ISSN:
1347-6947
Subject:
Northern blotting; Western blotting; biotechnology; mice; proteinases; small intestine; sucrose alpha-glucosidase
Abstract:
... The small intestinal sucrase activity in a senescence-accelerated strain of mouse, SAMP1, was significantly lower than that in other strains, including its control strain, SAMR1. In contrast, the activity of isomaltase, which usually associates with sucrase to form a complex enzyme (SI complex), in SAMP1 was comparable to that in other strains. Thus, the ratio of the sucrase to isomaltase activiti ...
DOI:
10.1271/bbb.62.965

73. Paraneoplastic pemphigus in a dog

Author:
Lemmens; De Bruin; De Meulemeester; Wyder; Suter
Source:
Veterinary dermatology 1998 v.9 no.2 pp. 127-134
ISSN:
0959-4493
Subject:
Western blotting; autoantibodies; biopsy; bladder; blood serum; cattle; dogs; ears; epithelium; erythema multiforme; females; fluorescent antibody technique; lymphoma; mouth; necropsy; nose; patients; skin lesions
Abstract:
... This report presents a case of paraneoplastic pemphigus in a 7‐year‐old female Bouvier. The dog initially showed extensive oral ulcerations that exacerbated upon treatment with trimethoprim‐sulfadiazine. Subsequently, the dog developed vesiculobullous and ulcerative lesions on the ear margins, the nose, periocular, and at the nail beds. Due to complete therapy resistance and a deteriorating genera ...
DOI:
10.1046/j.1365-3164.1998.00095.x

74. Plant Hsp100 family with special reference to rice

Author:
Sneh Lata Singla; Ashwani Pareek; Anil Grover
Source:
Journal of biosciences 1998 v.23 no.4 pp. 337-345
ISSN:
0250-5991
Subject:
Western blotting; antibodies; genes; heat shock proteins; heat stress; heat tolerance; rice; temperature; yeasts
Abstract:
... Heat shock proteins (Hsps) represent a group of specific proteins which are synthesized primarily in response to heat shock in almost all biological systems. Members of Hsp100 family have been directly implicated in induction of thermotolerance in microbial and animal cells. Yeast cells harbouring defectivehsp104 gene do not show thermotolerance under conditions in which the normal cells do. Sever ...
DOI:
10.1007/BF02936126

75. Production and characterization of a monoclonal antibody able to discriminate galectin-1 from galectin-2 and galectin-3

Author:
Jean Denis Cornillot; Michel Pontet; Catherine Dupuy; Ahmed Chadli; Michel Caron; Raymonde Joubert-Caron; Philippe Bourin; Dominique Bladier
Source:
Glycobiology 1998 v.8 no.5 pp. 425-432
ISSN:
1460-2423
Subject:
Western blotting; antiserum; brain; carbohydrates; clones; epitopes; galectins; humans; monoclonal antibodies; two-dimensional gel electrophoresis
Abstract:
... Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal origins but neither galectin-2 nor galectin-3. Western-blotting analysis of soluble h ...
DOI:
10.1093/glycob/8.5.425

76. Purification and characterization of recombinant spider silk expressed in Escherichia coli

Author:
Arcidiacono, S.; Mello, C.; Kaplan, D.; Cheley, S.; Bayley, H.
Source:
Applied microbiology and biotechnology 1998 v.49 no.1 pp. 31-38
ISSN:
0175-7598
Subject:
Escherichia coli; Nephila clavipes; Western blotting; amino acid composition; complementary DNA; desorption; genes; mass spectrometry; silk; silk proteins
Abstract:
... A partial cDNA clone, from the 3' end of the dragline silk gene was isolated from Nephila clavipes major ampullate glands. This clone contains a 1.7-kb insert, consisting of a repetitive coding region of 1.4-kb and a 0.3-kb nonrepetitive coding region; 1.5-kb of the 1.7-kb fragment was cloned into Escherichia coli and a 43-kDa recombinant silk protein was expressed. Characterization of the purifie ...
DOI:
10.1007/s002530051133
PubMed:
9487707

77. Purification of galectin-3 from ovine placenta: Developmentally regulated expression and immunological relevance

Author:
M. Mercedes Iglesias; Gabriel A. Rabinovich; Andrea L. Ambrosio; Leonardo F. Castagna; Claudia E. Sotomayor; Carlota Wolfenstein-Todel
Source:
Glycobiology 1998 v.8 no.1 pp. 59-65
ISSN:
1460-2423
Subject:
Western blotting; affinity chromatography; amino acid sequences; antiserum; chickens; cotyledons; enzymatic treatment; galectins; gel chromatography; gestation period; high performance liquid chromatography; isoelectric point; lactose; metastasis; molecular weight; morphogenesis; peptides; placenta; rabbits; rats; sheep; spleen
Abstract:
... Galectins, β-galactoside-binding lectins, are extensively distributed in the animal kingdom and share some basic molecular properties. Galectin-3, a member of this family, is generally associated with differentiation, morphogenesis, and metastasis. In this study, galectin-3 was isolated from ovine placental cotyledons round the middle of the gestation period by lactose extraction followed by affin ...
DOI:
10.1093/glycob/8.1.59
PubMed:
9451014

78. Quantification of cat immunoglobulins in the hemolymph of cat fleas (Siphonaptera: Pulicidae) after feeding on blood

Author:
Vaughan, J.A.; Thomas, R.E.; Silver, G.M.; Wisnewski, N.; Azad, A.F.
Source:
Journal of medical entomology 1998 v.35 no.4 pp. 404-409
ISSN:
0022-2585
Subject:
cats; Ctenocephalides felis; immunoglobulin G; hemolymph; hematophagy; host-parasite relationships; quantitative analysis; enzyme-linked immunosorbent assay; Western blotting
Abstract:
... Passage of ingested cat immunoglobulin G (IgG) into the hemocoel of cat fleas, Ctenocephalides felis (Bouche), was examined using antibody capture enzyme-linked immunosorbent assays (ELISA) and Western blotting. Fleas were fed heparinized cat blood via membrane feeders. Cat IgG was present in the hemolymph of engorged female fleas 1 h after ingestion at an estimated quantity of 35 +/- 14 microgram ...
DOI:
10.1093/jmedent/35.4.404

79. The acetylcholinesterase ichthyotoxin is a common component in the extracellular products of Vibrionaceae strains

Author:
Pérez; Rodríguez; Nieto
Source:
Journal of applied microbiology 1998 v.84 no.1 pp. 47-52
ISSN:
1364-5072
Subject:
Aeromonas hydrophila; Vibrio; Western blotting; acetylcholinesterase; colorimetry; fish; microbiology; neurotoxicity; neurotoxins; qualitative analysis
Abstract:
... In previous work, it was reported that a strain of Aeromonas hydrophila (B32) produces the most potent lethal toxin with neurotoxic activity described so far for fish. In the present study, the presence and distribution of this acetylcholinesterase toxin lethal for fish were determined in extracellular products (ECP) of 42 Vibrionaceae strains using both immunological and colorimetric methods. Thi ...
DOI:
10.1046/j.1365-2672.1997.00311.x

80. Accumulation of ppGpp in symbiotic and free-living nitrogen-fixing bacteria following amino acid starvation

Author:
Howorth, S.M.; England, R.R.
Source:
Archives of microbiology 1999 v.171 no.2 pp. 131-134
ISSN:
0302-8933
Subject:
nitrogen-fixing bacteria; cross reaction; Azorhizobium caulinodans; antibodies; Ensifer meliloti; adenosine triphosphate; Western blotting; amino acids; Azotobacter vinelandii; Rhizobium tropici; Azomonas agilis; Escherichia coli
Abstract:
... Following amino acid or ammonium starvation, ppGpp is accumulated by Rhizobium meliloti strain 1021 but not by R. meliloti strain 41 or Rhizobium tropici. Azorhizobium caulinodans ORS571 produced ppGpp following amino acid deprivation; however, the free-living nitrogen-fixing bacteria Azotobacter vinelandii and Azomonas agilis did not produce ppGpp. Western blot analysis using anti-RelA antibody d ...
DOI:
10.1007/s002030050689

81. Aldehyde oxidase in roots, leaves and seeds of barley (Hordeum vulgare L.)

Author:
Omarov, R.T.; Akaba, S.; Koshiba, T.; Lips, S.H.
Source:
Journal of experimental botany 1999 v.50 no.330 pp. 63-69
ISSN:
0022-0957
Subject:
metabolism; seeds; aldehyde oxidoreductases; Hordeum vulgare; immunohistochemistry; heat stability; enzyme activity; plant extracts; Western blotting; enzyme inhibitors; molybdenum; chemical constituents of plants; molecular weight; isozymes; roots; aldehydes; leaves
Abstract:
... Aldehyde oxidase (AO, EC 1.2.3.1) proteins in leaves, roots and seeds of barley (Hordeum vulgare L.) plants were studied. Differences in substrate specificity and mobility in native PAGE between AO proteins extracted from roots, leaves and seeds have been observed. Four clear bands of AO reacting proteins were detected in barley plants capable of oxidizing a number of aliphatic and aromatic aldehy ...
DOI:
10.1093/jxb/50.330.63

82. Ammonium can stimulate nitrate and nitrite reductase in the absence of nitrate in Clematis vitalba

Author:
Bungard, R.A.; Wingler, A.; Morton, J.D.; Andrews, M.; Press, M.C.; Scholes, J.D.
Source:
Plant, cell and environment 1999 v.22 no.7 pp. 859-866
ISSN:
0140-7791
Subject:
Clematis vitalba; Western blotting; barley; glutamate-ammonia ligase; glutamine; hydroponics; leaves; nitrate reductase; nitrates; nitrite reductase; nitrogen; nitrogen compounds; protein synthesis; shoots; tobacco
Abstract:
... Nitrogen assimilation was studied in the deciduous, perennial climber Clematis vitalba. When solely supplied with NO₃⁻ in a hydroponic system, growth and N-assimilation characteristics were similar to those reported for a range of other species. When solely supplied with NH₄⁺, however, nitrate reductase (NR) activity dramatically increased in shoot tissue, and particularly leaf tissue, to up to th ...
DOI:
10.1046/j.1365-3040.1999.00456.x

83. Cardiomyopathy in dystrophin-deficient hypertrophic feline muscular dystrophy

Author:
Gaschen, L.; Lang, J.; Lin, S.; Ade-Damilano, M.; Busato, A.; Lombard, C.W.; Gaschen, F.P.
Source:
Journal of veterinary internal medicine 1999 v.13 no.4 pp. 346-356
ISSN:
0891-6640
Subject:
mineralization; cardiomyopathy; radiography; cats; muscular dystrophy; contractile proteins; heterozygosity; Western blotting; myocardium; echocardiography
DOI:
10.1892/0891-6640(1999)013<0346:CIDDHF>2.3.CO;2
PubMed:
10449227

84. Ca²⁺ binding protein calreticulin in chlamydomonas reinhardtii (chlorophyta): biochemical characterization, differential expression during sexual reproduction, and phylogenetic analysis

Author:
Zuppini, Anna; Barbato, Roberto; Bergantino, Elisabetta; Dainese, Paola; Meggio, Flavio; Martin, William; Mariani, Paola
Source:
Journal of phycology 1999 v.35 no.6 pp. 1224-1232
ISSN:
0022-3646
Subject:
Chlamydomonas reinhardtii; Western blotting; amino acid sequences; calcium; calreticulin; dyes; endoplasmic reticulum; eukaryotic cells; gene expression regulation; germ cells; isoelectric point; messenger RNA; phylogeny; polyacrylamide gel electrophoresis; post-translational modification; sexual reproduction
Abstract:
... The occurrence of calreticulin, the main Ca²⁺ binding protein in the endoplasmic reticulum of eukaryotic cells, was investigated in the unicellular green alga Chlamydomonas reinhardtii Dangeard. The biochemical characterization of a diethylaminoethyl purified extract highlighted the presence, on SDS-PAGE, of a 55-kDa protein that stained blue with the Stains All dye, a diagnostic feature of acidic ...
DOI:
10.1046/j.1529-8817.1999.3561224.x

85. Characterisation of cellulose-binding proteins that are involved in the adhesion mechanism of Fibrobacter intestinalis DR7

Author:
Miron, J.; Forsberg, C.W.
Source:
Applied microbiology and biotechnology 1999 v.51 no.4 pp. 491-497
ISSN:
0175-7598
Subject:
Fibrobacter intestinalis; Western blotting; adhesion; antibodies; antiserum; bacteria; bacterial adhesion; cellulolytic microorganisms; cellulose; endo-1,4-beta-glucanase; galactosamine; galacturonic acid; glucosamine; glucuronic acid; glycosylation; high performance liquid chromatography; monosaccharides; mutants; oxidation; proteins
Abstract:
... Cellulose-binding proteins (CBP) isolated from cell envelopes of the cellulolytic bacterium Fibrobacter intestinalis strain DR7 were studied in order to investigate the adhesion mechanism. The proteins were examined for their reaction with antibodies that specifically block bacterial adhesion, response to glycosylation staining and monosaccharide composition. To this end, the effect of some monosa ...
DOI:
10.1007/s002530051422
PubMed:
10341432

86. Characterization of Antibody Response to Porcine Reproductive and Respiratory Syndrome Virus ORF5 Product following Infection and Evaluation of its Diagnostic Use in Pigs

Author:
Jimmy Kwang; Simon Yang; Fernando A. Osorio; Steve Christian; J. Galeota Wheeler; Kelly M. Lager; Sharon Low; Leo Chang; Alan R. Doster; Amy White; Ching Ching Wu
Source:
Journal of veterinary diagnostic investigation v.11 no.5 pp. 391-395
ISSN:
1040-6387
Subject:
swine; porcine reproductive and respiratory syndrome; Arterivirus; antibody formation; open reading frames; antibody detection; enzyme-linked immunosorbent assay; recombinant antigens; Western blotting
Abstract:
... The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negati ...
DOI:
10.1177/104063879901100501
PubMed:
12968750

87. Characterization of orthopoxviruses isolated from man and animals in Germany

Author:
H. Meyer; C. Schay; H. Mahnel; M. Pfeffer
Source:
Archives of virology 1999 v.144 no.3 pp. 491-501
ISSN:
0304-8608
Subject:
Cowpox virus; Southern blotting; Western blotting; antiserum; cats; chicken eggs; chorioallantoic membrane; dogs; humans; restriction mapping; Germany
Abstract:
... Fourteen orthopoxvirus strains isolated from humans, cats, a dog, a cow, and an elephant in Germany were characterized. All were classified as cowpox virus based on haemorrhagic lesions induced on the chorioallantoic membrane of chicken eggs and reactivity of a 160 kDa protein with anti-A-type inclusion protein hyperimmun serum in a Western blot. More detailed comparison of the isolates by restric ...
DOI:
10.1007/s007050050520
PubMed:
10226615

88. Cloning and expression of yellowtail ascites virus segment A

Author:
H. Sato; E. Emoto; T. Kamata; H. Mori; K. Kamei; A. Kitaoka; R. Takano; K. Nakajima; Y. Inui; K. Kudo; S. Hara
Source:
Archives of virology 1999 v.144 no.7 pp. 1405-1413
ISSN:
0304-8608
Subject:
Baculoviridae; Western blotting; amino acid sequences; complementary DNA; fat body; genes; insect larvae; insects; mice; open reading frames; proteins; viruses
Abstract:
... cDNA of yellowtail ascites virus (YAV) segment A encoding a polyprotein of VP2, NS, and VP3 has been cloned. Comparison of the nucleotide and the deduced amino acid sequences showed very high homology between YAV and other aquatic birnaviruses. The two small open reading frames (VP5) besides the 5′ terminus of the VP2 gene were found on segment A of YAV. Proteins encoded by cDNAs from segment A an ...
DOI:
10.1007/s007050050596
PubMed:
10481746

89. Control of metastasis by Asn-linked, β1–6 branched oligosaccharides in mouse mammary cancer cells

Author:
P. James Seberger; William G. Chaney
Source:
Glycobiology 1999 v.9 no.3 pp. 235-241
ISSN:
1460-2423
Subject:
Western blotting; caudal vein; cell lines; complementary DNA; cytotoxicity; genetic vectors; humans; lectins; lung neoplasms; mammary neoplasms (animal); membrane proteins; metastasis; mice; neoplasm cells; oligosaccharides; sialic acid; tissues; transcription (genetics); transferases
Abstract:
... Studies in cell lines and malignant human tissues have shown that increased cell-surface Asn-linked β1–6(GlcNAcβ1–6Man) branching is associated with increased tumorigenic and metastatic properties. In this study, three mouse mammary cancer cell lines were transfected with an expression vector containing the mouse cDNA for N-acetylglucosaminyltransferase V (GlcNAcT-V EC 2.4.1.155), the glycosyltran ...
DOI:
10.1093/glycob/9.3.235

90. Detection methods of possible prion contaminants in collagen and gelatin

Author:
T. Nemoto; M. Horiuchi; N. Ishiguro; M. Shinagawa
Source:
Archives of virology 1999 v.144 no.1 pp. 177-184
ISSN:
0304-8608
Subject:
Western blotting; collagen; gelatin; pollutants; polyethylene; prions; rodents; sodium chloride; viscosity
Abstract:
... We describe methods for the preparation of collagen and gelatin samples to detect possible prion contaminants using Western blotting of a major component of prions, PrPSᶜ. A commercially available collagen solution containing 2% athero-collagen was spiked with rodent adapted scrapie prion and used as the prion-contaminating collagen. The methods developed center on the enzymatic reduction of the c ...
DOI:
10.1007/s007050050494
PubMed:
10076518

91. Electrophoretic immunodesorption of proteins according to molecular weight by use of a double-membrane system

Author:
N. Abuharfeil; R. Atmeh; B. Shabsoug; M. Abo-Shehada
Source:
Chromatographia 1999 v.49 no.1-2 pp. 81-84
ISSN:
0009-5893
Subject:
Western blotting; affinity chromatography; agarose; antiserum; autoradiography; blood serum; cellophane; cellulose acetate; denaturation; desorption; dialysis; gel electrophoresis; human serum albumin; humans; molecular weight; porosity; radiolabeling; sodium dodecyl sulfate
Abstract:
... A simple method is described for electrophoretic desorption of proteins from antigen-antibody complexes, with more than 90% recovery and without denaturation, after immunosorbent affinity chromatography. Radiolabeled or unlabeled human serum albumin (HSA) and α-1-antitrypsin (AAT), conjugated to rabbit anti-HSA or anti-AAT polyclonal antisera, respectively, were electrophoretically desorbed from S ...
DOI:
10.1007/BF02467192

92. Expression of a novel human sialidase encoded by the NEU2 gene

Author:
Eugenio Monti; Augusto Preti; Carlo Nesti; Andrea Ballabio
Source:
Glycobiology 1999 v.9 no.12 pp. 1313-1321
ISSN:
1460-2423
Subject:
Western blotting; active sites; amino acid sequences; amino acids; cell fractionation; chromosomes; enzyme activity; genes; glycolipids; glycoproteins; humans; immunohistochemistry; molecular weight; pH; polyclonal antibodies; polypeptides; prediction; rabbits; recombinant proteins; sialic acid; sialidase
Abstract:
... Sialidases (E.C.3.2.1.18) belong to a group of glycohydrolytic enzymes, widely distributed in nature, which remove sialic acid residues from glycoproteins and glycolipids. All of the sialidase so far characterized at the molecular level share an Asp block, repeated three to five times in the primary structure, and an F/YRIP sequence motif which is part of the active site. Using a sequence homology ...
DOI:
10.1093/glycob/9.12.1313

93. Expression of the Lewis group carbohydrate antigens during Xenopus development

Author:
Chikako Yoshida-Noro; Janet Heasman; Kim Goldstone; Lucinda Vickers; Chris Wylie
Source:
Glycobiology 1999 v.9 no.12 pp. 1323-1330
ISSN:
1460-2423
Subject:
Western blotting; Xenopus laevis; adults; animal ovaries; antibodies; antigens; brain; cell adhesion; cement glands; digestive system; embryo (animal); endothelial cells; glycoproteins; head; kidneys; leukocytes; ligands; lungs; mice; muscles; neurites; retina; spleen; stem cells; tadpoles; testes; tissues; toads
Abstract:
... We have examined the pattern of expression of the Lewis group carbohydrate antigens during the development of African toad Xenopus laevis. One of these antigens, Lewis x (Leˣ, also known as SSEA-1), was previously shown to be involved in cell-cell adhesion in early mouse embryos and teratocarcinoma stem cells. Recently another member of these antigens, sialyl-Leˣ, was found to be one of the major ...
DOI:
10.1093/glycob/9.12.1323

94. Expression of the insulin-like growth factor 1 receptor (IGF-1R) in breast cancer cells: evidence for a regulatory role of dolichyl phosphate in the transition from an intracellular to an extracellular IGF-1 pathway

Author:
Anica Dricu; Lena Kanter; Min Wang; Gunnar Nilsson; Magnus Hjertman; Johan Wejde; Olle Larsson
Source:
Glycobiology 1999 v.9 no.6 pp. 571-579
ISSN:
1460-2423
Subject:
Western blotting; antibodies; autocrine signaling; breast neoplasms; cell growth; cell lines; estrogens; glycosylation; insulin-like growth factor I; insulin-like growth factor I receptor; melanoma; membrane proteins; neoplasm cells; phosphates; plasma membrane; protein phosphorylation; receptors; tritium
Abstract:
... In this study we provide evidence that the low expression of IGF-1R at the cell surface of estrogen-independent breast cancer cells is due to a low rate of e novo synthesis of dolichyl phosphate. The analyses were performed on the estrogen receptor-negative breast cancer cell line MDA231 and, in comparison, the melanoma cell line SK-MEL-2, which expresses a high number of plasma membrane-bound IGF ...
DOI:
10.1093/glycob/9.6.571

95. Growth and Differentiation of Cultured Fetal Hepatocytes Isolated from Various Developmental Stages

Author:
Ryuji HAMAMOTO; Masamichi KAMIHIRA; Shinji IIJIMA
Source:
Bioscience, biotechnology, and biochemistry 1999 v.63 no.2 pp. 395-401
ISSN:
1347-6947
Subject:
Western blotting; albumins; cell growth; cell proliferation; hepatocytes; in vitro culture; liver; neonates; pregnancy; rats; transcription factors; tumor suppressor genes
Abstract:
... We examined the relationship between cell proliferation and differentiation of cultured rat fetal and newborn hepatocytes isolated from various developmental stages. The albumin production rate increased along with cell growth under in vitro culture and became maximal two days after the growth cessation. AFP was secreted by both fetal and newborn hepatocytes with growth ability. Furthermore, the r ...
DOI:
10.1271/bbb.63.395

96. Hemagglutinating and acid phosphatase (AcPASE) activities in developing seedlings of four species of Cucurbitaceae

Author:
Irena Lorenc-Kubis; Urszula Uram-Walaszczyk
Source:
Acta Societatis Botanicorum Poloniae 1999 v.68 no.4 pp. 291-297
ISSN:
0001-6977
Subject:
hemagglutination; Cucurbita maxima; concanavalin A; acid phosphatase; germination; Cucumis sativus; antibodies; seedlings; Fabaceae; cotyledons; Western blotting; Cucurbita pepo
Abstract:
... The acid phosphatase and hemagglutinating activities of four species of Cucurbitaceae were determined during seeds germination and seedlings development. In all cases traces of enzyme and hemagglutinating activities were found in dry and imbibided seeds. In developing seedlings of Cucumis sativus the activities increased to maximum on the 3rd day while in other species on the 6th day of germinatio ...
DOI:
10.5586/asbp.1999.041

97. Identification and characterization of potential new therapeutic targets in inflammatory and autoimmune diseases

Author:
Ursula Mangold; Claudia I. Dax; Karin Saar; Wilfried Schwab; Bernhard Kirschbaum; Stefan Müllner
Source:
European journal of biochemistry 1999 v.266 no.3 pp. 1184-1191
ISSN:
0014-2956
Subject:
Western blotting; affinity chromatography; anti-inflammatory agents; autoimmune diseases; binding proteins; drug interactions; glyceraldehyde-3-phosphate dehydrogenase; glycolysis; macrophages; mechanism of action; metabolites; phosphoglycerate kinase; pyruvate kinase; sequence analysis
Abstract:
... The isoxazole derivative Leflunomide (HWA 486) is a novel immunoregulatory and anti‐inflammatory drug. Affinity chromatography was used to purify and identify Leflunomide binding proteins, which might play a role as potential cellular targets in the molecular mode of action. The Leflunomide derivative A 0273 was covalently coupled to a Fractogel® matrix. This column was used to separate a cytosoli ...
DOI:
10.1046/j.1432-1327.1999.00978.x

98. Implications of hemolin glycosylation and Ca²⁺‐binding on homophilic and cellular interactions

Author:
Raul Bettencourt; Hans Gunne; Louis Gastinel; Håkan Steiner; Ingrid Faye
Source:
European journal of biochemistry 1999 v.266 no.3 pp. 964-976
ISSN:
0014-2956
Subject:
Hyalophora cecropia; N-acetylglucosamine; Western blotting; adaptive immunity; antibodies; autoradiography; calcium; cell adhesion; digestion; ethylene glycol tetraacetic acid; fluorescence; glycosylation; hemocytes; immune response; lectins; models; moths; pupae; receptors; recombinant proteins; sialic acids; sialidase
Abstract:
... Insects are useful models for the study of innate immune mechanisms because of their lack of antibodies and receptors involved in adaptive immune response. Nevertheless, hemolin cloned from moths is a soluble and membrane associated Ig‐related molecule that is up‐regulated during immune response [Lanz‐Mendoza, H. & Faye, I. (1999) Dev. Comp. Immunol.23, 359–374]. The hemolin monomeric form has fou ...
DOI:
10.1046/j.1432-1327.1999.00934.x

99. Insulin activates Gαil,2 protein in rat hepatoma (HTC) cell membranes

Author:
V. Sánchez-Margalet; C. González-Yanes; J. Santos-Alvarez; S. Najib
Source:
Cellular and molecular life sciences 1999 v.55 no.1 pp. 142-147
ISSN:
1420-682X
Subject:
Western blotting; antibodies; dose response; guanosine triphosphate; hepatoma; insulin; insulin receptors; membrane proteins; pertussis toxin; plasma membrane; rats; signal transduction
Abstract:
... Insulin action is initiated by binding to its cognate receptor, which then triggers multiple cellular responses by activating different signaling pathways. There is evidence that insulin receptor signaling may involve G protein activation in different target cells. We have studied the activation of G proteins in rat hepatoma (HTC) cells. We found that insulin stimulated binding of guanosine 5′-O-( ...
DOI:
10.1007/s000180050279

100. Insulin‐like growth factor‐I (IGF‐I)‐dependent activation of pp42/44 mitogen‐activated protein kinase occurs independently of IGF‐I receptor kinase activation and IRS‐1 tyrosine phosphorylation

Author:
Lorraine Yau; Helena Lukes; Heather McDiarmid; Julieta Werner; Peter Zahradka
Source:
European journal of biochemistry 1999 v.266 no.3 pp. 1147-1157
ISSN:
0014-2956
Subject:
DNA; RNA; Western blotting; binding sites; cell proliferation; glucose; hepatoma; insulin; insulin receptors; insulin-like growth factor I; insulin-like growth factor I receptor; metabolism; mitogen-activated protein kinase; mitogen-activated protein kinase kinase; mitogen-activated protein kinase kinase kinase; phosphorylation; tyrosine
Abstract:
... The proliferation and metabolism of H4IIE hepatoma cells is apparently mediated through the insulin receptor. These cells, however, also have high‐affinity binding sites for insulin‐like growth factor‐I (IGF‐I). Addition of insulin to H4IIE cells increased RNA synthesis, DNA synthesis and cell number. IGF‐I, on the other hand, was ineffective at concentrations equivalent to the lowest effective in ...
DOI:
10.1046/j.1432-1327.1999.00968.x