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1. (-) - Epicatechin improves Tibialis anterior muscle repair in CD1 mice with BaCl2-induced damage

Author:
Magally Ramírez-Ramírez; Francisca Fernández-Valverde; Andrea Reséndiz-García; Mirna G Martínez-Damas; Luis Javier Cano-Martínez; Alejandro Zentella-Dehesa; Ramón M Coral-Vázquez
Source:
Journal of nutritional biochemistry 2022 v.107 pp. 109069
ISSN:
0955-2863
Subject:
Western blotting; animal models; antioxidants; diabetes; epicatechin; histology; hypertrophy; mice; muscle development; muscles; muscular dystrophy; myogenin; sarcopenia; skeletal muscle; therapeutics
Abstract:
... Several studies have shown the beneficial effect that Epicatechin (Epi) has on the skeletal muscle of murine models and patients with muscular dystrophy and in the muscles of patients with diabetes or murine sarcopenia models. This flavanol has been shown to enhance antioxidant pathways and improve muscle architecture. However, the repair process during muscle regeneration has not been analyzed. T ...
DOI:
10.1016/j.jnutbio.2022.109069

2. (-)-7(S)-hydroxymatairesinol protects against tumor necrosis factor-α-mediated inflammation response in endothelial cells by blocking the MAPK/NF-κB and activating Nrf2/HO-1

Author:
Di Yang; Chen-Xi Xiao; Zheng-Hua Su; Meng-Wei Huang; Ming Qin; Wei-Jun Wu; Wan-Wan Jia; Yi-Zhun Zhu; Jin-Feng Hu; Xin-Hua Liu
Source:
Phytomedicine 2017 v.32 pp. 15-23
ISSN:
0944-7113
Subject:
Western blotting; anti-inflammatory activity; antineoplastic activity; antioxidants; cardiovascular diseases; drugs; endothelial cells; fluorescent antibody technique; gene expression regulation; heme oxygenase (biliverdin-producing); inducible nitric oxide synthase; inflammation; interleukin-6; lignans; messenger RNA; mitogen-activated protein kinase; necrosis; pathogenesis; phosphorylation; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; superoxide dismutase; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Endothelial inflammation is an increasingly prevalent condition in the pathogenesis of many cardiovascular diseases. (-)-7(S)-hydroxymatairesinol (7-HMR), a naturally occurring plant lignan, possesses both antioxidant and anti-cancer properties and therefore would be a good strategy to suppress tumor necrosis factor-α (TNF-α)-mediated inflammation in vascular endothelial cells (VECs).: The objecti ...
DOI:
10.1016/j.phymed.2017.04.005

3. (-)-Epigallocatechin-3-gallate suppresses cigarette smoke-induced inflammation in human cardiomyocytes via ROS-mediated MAPK and NF-κB pathways

Author:
Yingmin Liang; Mary Sau Man Ip; Judith Choi Wo Mak
Source:
Phytomedicine 2019 v.58 pp. 152768
ISSN:
0944-7113
Subject:
Camellia sinensis; Western blotting; acetylcysteine; apoptosis; cardiomyocytes; cardioprotective agents; cardiovascular diseases; catechin; chemokines; cigarettes; enzyme-linked immunosorbent assay; epigallocatechin gallate; flow cytometry; green tea; humans; inflammation; interleukins; leaves; mitochondria; mitogen-activated protein kinase; oxidative stress; reactive oxygen species; smoking (habit); transcription factor NF-kappa B
Abstract:
... Cigarette smoking is the leading cause for the initiation and development of cardiovascular disease (CVD). Oxidative stress and inflammatory responses play important roles in the pathophysiological processes of smoking-induced cardiac injury. (-)-epigallocatechin-3-gallate (EGCG), the most abundant catechin in green tea, which is made from Camellia sinensis leaves, has been reported to possess pot ...
DOI:
10.1016/j.phymed.2018.11.028

4. (-)-Pinoresinol monomethyl ether inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-κB in Raw 264.7 macrophage cells

Author:
Bo-Young Park; Mee-Young Lee; Kyung-Seop Ahn; Young-Won Chin; Hyeong-Kyu Lee; Sei-Ryang Oh
Source:
Han'guk Ŭngyong Saengmyŏng Hwahakhoe chi = 2011 v.54 no.2 pp. 163-168
ISSN:
1738-2203
Subject:
Western blotting; anti-inflammatory activity; inducible nitric oxide synthase; lipopolysaccharides; macrophages; nitric oxide; prostaglandin synthase; prostaglandins; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Anti-inflammatory effects of (-)-pinoresinol monomethyl ether (PME) were evaluated on lipopolysaccharide (LPS)-treated Raw 264.7 macrophage cells. PME inhibited translocation of p65-nuclear factor-κB (NF-κB) into the nucleus in immunocytochemical analysis for NF-κB activation, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in Western blotting, as well as producti ...
DOI:
10.3839/jksabc.2011.027

5. (-)-Syringaresinol-4-O-β-D-glucopyranoside from Cortex Albizziae inhibits corticosterone-induced PC12 cell apoptosis and relieves the associated dysfunction

Author:
Desen Yang; Wanqin Wu; Guoping Gan; Dingkun Wang; Jing Gong; Ke Fang; Fuer Lu
Source:
Food and chemical toxicology 2020 v.141 pp. 111394
ISSN:
0278-6915
Subject:
Western blotting; anxiety; apoptosis; binding proteins; calcium; caspase-3; cell proliferation; cell viability; cortex; corticosterone; cytochrome c; enzyme activity; flow cytometry; glycosides; lactate dehydrogenase; lignans; membrane potential; mitochondria; mitochondrial membrane; models; neuroprotective effect; pathogenesis; propidium; protein synthesis; rhodamines; staining; toxicology
Abstract:
... The neuroprotective effects and potential mechanisms of (-)-Syringaresinol-4-O-β-D-glucopyranoside (SRG), a natural lignan glycoside extracted from Cortex Albizziae, were investigated using corticosterone (CORT)-induced PC12 cells as an in vitro anxiety model. PC12 cells were treated with 100 μM CORT and 5, 10, or 20 μM SRG for 48 h. Cell viability and lactate dehydrogenase (LDH) leakage were meas ...
DOI:
10.1016/j.fct.2020.111394

6. (2S)-2′-Methoxykurarinone from Sophora flavescens Suppresses Cutaneous T Cell-Attracting Chemokine/CCL27 Expression Induced by Interleukin-β/Tumor Necrosis Factor-α via Heme Oxygenase-1 in Human Keratinocytes

Author:
Jeong, Seung-Il; Lee, Young-Eun; Jang, Seon Il
Source:
Journal of medicinal food 2010 v.13 no.5 pp. 1116-1124
ISSN:
1096-620X
Subject:
heme oxygenase (biliverdin-producing); humans; interleukins; oxidative stress; inflammation; reverse transcriptase polymerase chain reaction; Western blotting; enzyme-linked immunosorbent assay; tumor necrosis factor-alpha; carbon monoxide; atopic dermatitis; keratinocytes; antioxidant activity; chemokines; Sophora flavescens; contact dermatitis; small interfering RNA
Abstract:
... One of the CC chemokines, cutaneous T cell-attracting chemokine (CTACK/CCL27), is a skin-specific CC chemokine that is produced constitutively by keratinocytes and is highly up-regulated in inflammatory skin conditions such as atopic dermatitis and contact dermatitis. (2S)-2′-Methoxykurarinone (MOK) from Sophora flavescens has been demonstrated to have antioxidant effects. Heme oxygenase (HO)-1 ha ...
DOI:
10.1089/jmf.2009.1333

7. (2S)-7,4′-dihydroxy-8-prenylflavan stimulates adipogenesis and glucose uptake through p38MAPK pathway in 3T3-L1 cells

Author:
Jun Ji; Jingjie Zhu; Xiao Hu; Ting Wang; Xiaodong Zhang; Ai-Jun Hou; Heyao Wang
Source:
Biochemical and biophysical research communications 2015 v.460 pp. 578-582
ISSN:
0006-291X
Subject:
Western blotting; adipogenesis; adiponectin; adipose tissue; genes; glucose; glucose transporters; lipids; mitogen-activated protein kinase; noninsulin-dependent diabetes mellitus; obesity; quantitative polymerase chain reaction; tumor necrosis factor-alpha
Abstract:
... Adipose tissue plays a key role in the development of obesity and diabetes. Natural products are one of the main sources for discovering new lead compounds. In the present study, (2S)-7,4′-dihydroxy-8-prenylflavan (DHPF), a natural prenylated flavan isolated from Morus yunnanensis, was found to significantly promote adipogenesis and increase glucose uptake in 3T3-L1 cells. Real-time PCR results sh ...
DOI:
10.1016/j.bbrc.2015.03.072

8. (7R,8S)-9-Acetyl-dehydrodiconiferyl alcohol inhibits inflammation and migration in lipopolysaccharide-stimulated macrophages

Author:
Li-Long Pan; Qiu-Yang Zhang; Xiao-Ling Luo; Juan Xiong; Peng Xu; Si-Yu Liu; Jin-Feng Hu; Xin-Hua Liu
Source:
Phytomedicine 2016 v.23 no.5 pp. 541-549
ISSN:
0944-7113
Subject:
Clematis; Western blotting; alcohols; anti-inflammatory activity; cell movement; chemotaxis; inducible nitric oxide synthase; inflammation; lignans; lipopolysaccharides; macrophages; mitogen-activated protein kinase; nitric oxide; non-specific protein-tyrosine kinase; non-specific serine/threonine protein kinase; phosphorylation; prostaglandin synthase; stems; transcription factor NF-kappa B; viability
Abstract:
... (7R, 8S)-9-Acetyl-dehydrodiconiferyl alcohol (ADDA), a novel lignan compound isolated from Clematis armandii Franch (Ranunculaceae) stems, has been found to exert potential anti-inflammatory activities in vitro.To investigate the pharmacological effects and molecular mechanisms of ADDA on lipopolysaccharide (LPS)-induced activation and migration of macrophages.Macrophages were stimulated with LPS ...
DOI:
10.1016/j.phymed.2016.02.018

9. (±)-Equol interferes with the secretion of gonadotropin-releasing hormone and the expression of neuromodulators in murine neurons

Author:
Jingyuan Xiong; Minghui Wang; Ye Tian; Aru Ling; Shufang Shan; Li Zhao; Guo Cheng
Source:
Toxicological and environmental chemistry 2022 v.104 no.1 pp. 129-140
ISSN:
1029-0486
Subject:
RING finger domains; Western blotting; cell viability; daidzein; environmental science; enzyme-linked immunosorbent assay; estrogens; females; gonadotropin-releasing hormone; intestines; menopause; metabolites; mice; neurotransmitters; quantitative polymerase chain reaction; secretion; toxicology
Abstract:
... S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the ef ...
DOI:
10.1080/02772248.2022.2055031

10. (‐)‐Epicatechin gallate, a metabolite of (‐)‐epigallocatechin gallate in grass carp, exhibits antiviral activity in vitro against grass carp reovirus

Author:
Yitong Zhang; Hao Wang; Meizhen Su; Liqun Lu
Source:
Aquaculture research 2020 v.51 no.4 pp. 1673-1680
ISSN:
1355-557X
Subject:
Ctenopharyngodon idella; Reoviridae; Western blotting; antiviral agents; antiviral properties; aquaculture; catechin; cytopathogenicity; epigallocatechin gallate; green tea; kidneys; metabolites; moieties; pharmacokinetics; stress response
Abstract:
... (‐)‐Epigallocatechin gallate (EGCG), a catechin found in green tea, has been demonstrated to exhibit activity against grass carp reovirus (GCRV). In the current study, we found that EGCG is partially transformed in vivo into (‐)‐epicatechin gallate (ECG), which differs from EGCG only by the absence of a hydroxyl group, and exhibits similar pharmacokinetic behaviour to that of EGCG. ECG is also a m ...
DOI:
10.1111/are.14513

11. (−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma

Author:
I-Ta Lu; Shih-Chao Lin; Yi-Chia Chu; Ya Wen; You-Cheng Lin; Wen-Chien Cheng; Jyh-Horng Sheu; Chi-Chien Lin
Source:
Marine drugs 2022 v.20 no.2 pp. -
ISSN:
1660-3397
Subject:
Western blotting; antibacterial properties; apoptosis; caspases; cell viability; death domain receptors; endoplasmic reticulum; guanidines; hepatoma; humans; liver; membrane potential; methanol; mitochondria; mitochondrial membrane; sesquiterpenoids
Abstract:
... Liver cancers, such as hepatocellular carcinoma (HCC), are a highly prevalent cause of cancer-related deaths. Current treatments to combat liver cancer are limited. (−)-Agelasidine A, a compound isolated from the methanol extract of Agelasnakamurai, a sesquiterpene guanidine derived from sea sponge, has antibacterial activity. We demonstrated its anticancer capabilities by researching the associat ...
DOI:
10.3390/md20020109

12. (−)-Epicatechin Inhibits Development Of Dilated Cardiomyopathy In Δ Sarcoglycan Null Mouse

Author:
Sergio De los Santos; Carlos Palma-Flores; Alejandro Zentella-Dehesa; Patricia Canto; Ramón M. Coral-Vázquez
Source:
Nutrition, metabolism, and cardiovascular diseases 2018 v. no. pp. -
ISSN:
0939-4753
Subject:
Western blotting; atrial natriuretic peptide; brain; cardiomyopathy; coronary vessels; endothelial nitric oxide synthase; fibrosis; flavonols; heart; heart failure; histology; metabolism; mice; morphometry; mutants; phosphorylation; proteins; therapeutics
Abstract:
... Several studies propose that (−)-epicatechin, a flavonol present in high concentration in the cocoa, has cardioprotective effects.This study aimed to evaluate the impact of (−)-epicatechin on the development of dilated cardiomyopathy in a δ sarcoglycan null mouse model.δ sarcoglycan null mice were treated for 15 days with (−)-epicatechin. Histological and morphometric analysis of the hearts treate ...
DOI:
10.1016/j.numecd.2018.06.019

13. (−)-Epicatechin attenuates high-glucose-induced inflammation by epigenetic modulation in human monocytes

Author:
Isabel Cordero-Herrera; Xinpu Chen; Sonia Ramos; Sridevi Devaraj
Source:
European journal of nutrition 2017 v.56 no.3 pp. 1369-1373
ISSN:
1436-6207
Subject:
Western blotting; acetylation; chromatin; culture media; diabetes; epigenetics; flavonols; glucose; glycemic effect; histone acetyltransferase; histones; humans; inflammation; monocytes; polyphenols; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... PURPOSE: Diabetes is a pro-inflammatory state associated with increased monocyte activity. NF-κB is the master switch of inflammation and is activated during diabetes. (−)-Epicatechin (EC), the main cocoa flavonol, displays anti-inflammatory and anti-diabetic effects under high glucose conditions. Recently, it has been suggested that dietary polyphenols might modulate chromatin remodelling by epig ...
DOI:
10.1007/s00394-015-1136-2

14. (−)-Epicatechin modulates the expression of myomiRs implicated in exercise response in mouse skeletal muscle

Author:
Carlos Palma-Flores; Paola B. Zárate-Segura; J. Manuel Hernández-Hernández; Sergio de los Santos; Andrea S. Tejeda-Gómez; Luis Javier Cano-Martínez; Patricia Canto; Jorge Omar Garcia-Rebollar; Ramón M. Coral-Vázquez
Source:
Gene 2023 v.849 pp. 146907
ISSN:
0378-1119
Subject:
Western blotting; bioinformatics; exercise; genes; hypertrophy; mice; microRNA; muscle development; muscles; myogenin; signal transduction; skeletal muscle
Abstract:
... The flavanol (−)-epicatechin has exercise-mimetic properties. Besides, several miRNAs play a role in modulating the adaptation of the muscle to different training protocols. However, notwithstanding all information, few studies aimed to determine if (−)-epicatechin can modify the expression of miRNAs related to skeletal muscle development and regeneration. Mice were treated for fifteen days by ora ...
DOI:
10.1016/j.gene.2022.146907

15. (−)-Epigallocatechin-3-gallate (EGCG) inhibits fibrillation, disaggregates amyloid fibrils of α-synuclein, and protects PC12 cells against α-synuclein-induced toxicity

Author:
Juan Zhao; Qingnan Liang; Qing Sun; Congheng Chen; Lihui Xu; Yu Ding; Ping Zhou
Source:
RSC advances 2017 v.7 no.52 pp. 32508-32517
ISSN:
2046-2069
Subject:
Lewy bodies; Parkinson disease; Western blotting; amyloid; atomic force microscopy; blood-brain barrier; brain; cell death; circular dichroism spectroscopy; confocal laser scanning microscopy; dose response; epigallocatechin gallate; fluorescence emission spectroscopy; nuclear magnetic resonance spectroscopy; patients; protective effect; toxicity; transmission electron microscopy
Abstract:
... α-Synuclein (α-Syn) aggregates are the major component of Lewy bodies (LB), which is a pathological hallmark in the brain tissue of Parkinson's disease (PD) patients. It has been reported that (−)-epigallocatechin-3-gallate (EGCG) is biologically able to penetrate the blood–brain barrier and inhibit the fibrillation of amyloid proteins. This study aimed to provide insight into the possible mechani ...
DOI:
10.1039/c7ra03752j

16. (−)-Epigallocatechin-3-gallate Enhances the Expression of an Insulin-Inducible Transcription Factor Gene via a Phosphoinositide 3-Kinase/Atypical Protein Kinase C Lambda Pathway

Author:
Asano, Kosuke; Takagi, Katsuhiro; Haneishi, Ayumi; Yamamoto, Taichi; Tanaka, Takashi; Noguchi, Tamio; Nakamura, Soichiro; Yamada, Kazuya
Source:
Journal of agricultural and food chemistry 2011 v.59 no.24 pp. 13360–13364
ISSN:
0021-8561
Subject:
Western blotting; gene expression; genes; green tea; liver; messenger RNA; phosphatidylinositol 3-kinase; phosphorylation; protein kinase C; rats; signal transduction; transcription (genetics); transcription factors
Abstract:
... The rat enhancer of split- and hairy-related protein-1 (SHARP-1) is an insulin-inducible transcriptional repressor. In this study, we examined issues of whether (−)-epigallocatechin-3-gallate (EGCG), a green tea polyphenol, regulates the expression of the rat SHARP-1 gene and which signaling pathway mediates the regulation. When H4IIE cells were treated with EGCG, SHARP-1 mRNA levels rapidly incre ...
DOI:
10.1021/jf204016k

17. (−)-Epigallocatechin-3-gallate inhibits matrix metalloproteinases in oral ulcers

Author:
Bin Huang; Huangqin Chen
Source:
RSC advances 2015 v.5 no.30 pp. 23758-23766
ISSN:
2046-2069
Subject:
Western blotting; acetic acid; animal models; colorimetry; dose response; enzyme-linked immunosorbent assay; epigallocatechin gallate; extracellular matrix; fluorometry; immunohistochemistry; interleukin-1beta; luciferase; macrophages; metalloproteinases; quantitative polymerase chain reaction; transcription (genetics)
Abstract:
... The overexpression of matrix metalloproteinases (MMPs) results in excessive extracellular matrix degradation and oral ulcer healing delay. This study investigated the inhibitory effect of (−)-epigallocatechin-3-gallate (EGCG) on MMPs, the possible molecular mechanism and its implications for oral ulcers. MMP-1 and MMP-9 inhibition was detected using fluorometric and colorimetric assays after incub ...
DOI:
10.1039/c5ra01263e

18. (−)-Oleocanthal as a Dual c-MET-COX2 Inhibitor for the Control of Lung Cancer

Author:
Abu Bakar Siddique; Phillip C.S.R. Kilgore; Afsana Tajmim; Sitanshu S. Singh; Sharon A. Meyer; Seetharama D. Jois; Urska Cvek; Marjan Trutschl; Khalid A. El Sayed
Source:
Nutrients 2020 v.12 no.6 pp. -
ISSN:
2072-6643
Subject:
Western blotting; adenocarcinoma; assays; caudal vein; dietary supplements; dose response; epithelium; extra-virgin olive oil; genes; incidence; injection; lead; lung neoplasms; lungs; metastasis; mice; microarray technology; models; oral administration; prostaglandin synthase; survival rate
Abstract:
... Lung cancer (LC) represents the topmost mortality-causing cancer in the U.S. LC patients have overall poor survival rate with limited available treatment options. Dysregulation of the mesenchymal epithelial transition factor (c-MET) and cyclooxygenase 2 (COX2) initiates aggressive LC profile in a subset of patients. The Mediterranean extra-virgin olive oil (EVOO)-rich diet already documented to re ...
DOI:
10.3390/nu12061749

19. 1, 25(OH)2D3 protects β cell against high glucose-induced apoptosis through mTOR suppressing

Author:
Zesong Yang; Fang Liu; Hua Qu; Hang Wang; Xiaoqiu Xiao; Huacong Deng
Source:
Molecular and Cellular Endocrinology 2015 v.414 pp. 111-119
ISSN:
0303-7207
Subject:
Western blotting; apoptosis; caspases; cytochrome c; death; diabetes mellitus; flow cytometry; gene expression regulation; glucose; islets of Langerhans; mammals; pathogenesis; patients; public health; reverse transcriptase polymerase chain reaction; risk factors; signal transduction; therapeutics; vitamin D deficiency; China
Abstract:
... Diabetes mellitus is a leading cause of death and disability worldwide, which presents a serious public health crisis in China nowadays. It has been well recognized that excessive β-cell apoptosis is the key pathogenesis of diabetes, of which the mammalian target of rapamycin (mTOR) serves as the critical signaling pathway. Emerging evidence indicates that vitamin D deficiency acts as a potential ...
DOI:
10.1016/j.mce.2015.07.023

20. 1, 6-di-O-caffeoyl-β-D-glucopyranoside, a natural compound from Callicarpa nudiflora Hook impairs P2Y12 and thromboxane A2 receptor-mediated amplification of platelet activation and aggregation

Author:
Jianjiang Fu; Xiaocui Zhu; Wei Wang; Hong Lu; Zhoumiao Zhang; Ting Liu; Huanjun Xu; Huizheng Fu; Shuangcheng Ma; Yuehua Luo
Source:
Phytomedicine 2017 v.36 pp. 273-282
ISSN:
0944-7113
Subject:
Callicarpa; Western blotting; adenosine diphosphate; arachidonic acid; atherosclerosis; integrins; ligands; myocardial infarction; platelet aggregation; radiolabeling; rats; receptors; secretion; serotonin; signal transduction; thrombosis; thromboxanes
Abstract:
... Platelet activation and subsequent accumulation at sites of vascular injury perform a central role in thrombus formation, which is believed to be the trigger of several cardiovascular diseases, such as atherosclerosis, myocardial infarction and strokes. In this sense, the search for agents that are capable of blocking platelets aggregation has important implications for these diseases. Callicarpa ...
DOI:
10.1016/j.phymed.2017.10.012

21. A 1,10-phenanthroline derivative selectively targeting telomeric G-quadruplex induces cytoprotective autophagy, causing apoptosis of gastric cancer cells

Author:
Xudong Ma; Annoor Awadasseid; Kang Zhou; Xiao Wang; Chenfeng Shen; Xiaoyin Zhao; Mei Cheng; Wen Zhang
Source:
Life sciences 2021 v.287 pp. 120095
ISSN:
0024-3205
Subject:
DNA damage; Western blotting; antineoplastic activity; apoptosis; autophagy; cell proliferation; energy transfer; flow cytometry; fluorescence; fluorescent antibody technique; genes; ligands; signal transduction; stomach neoplasms; telomerase; telomeres; transmission electron microscopy
Abstract:
... This study aimed to evaluate the ability of compound 13d to induce autophagy and to promote apoptosis of tumor cells and its interaction mechanism.Using CCK-8 assay, transwell assay, fluorescence resonance energy transfer melting analysis (FRET), transmission electron microscopy, flow cytometry assay, immunofluorescence assay, Western blot analysis, and wound healing assay.The results indicated th ...
DOI:
10.1016/j.lfs.2021.120095

22. 1,25(OH)2D3 induced apoptosis of human hepatocellular carcinoma cells in vitro and inhibited their growth in a nude mouse xenograft model by regulating histone deacetylase 2

Author:
Jian Huang; Guozhen Yang; Yunzhu Huang; Shu Zhang
Source:
Biochimie 2018 v.146 pp. 28-34
ISSN:
0300-9084
Subject:
Western blotting; animal models; apoptosis; caspase-8; flow cytometry; hepatoma; histone deacetylase; histopathology; human cell lines; humans; mice; necrosis; neoplasm cells; proteins; quantitative polymerase chain reaction; staining; tissues
Abstract:
... The aim of this study was to evaluate the effects of 1,25(OH)2D3 on the apoptosis of human hepatocellular carcinoma (HCC) cells. The effects of 1,25(OH)2D3 on the proliferation and apoptosis of human HCC cells by regulating histone deacetylase 2 (HDAC2) were assessed by MTT assay and flow cytometry. The effects of 1,25(OH)2D3 on the expressions of related proteins in the apoptosis pathway by regul ...
DOI:
10.1016/j.biochi.2017.11.012

23. 1,25-(OH)2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway

Author:
Shiqing Xu; Jing Li; Min Zhai; Xiaoqi Yao; Honglin Liu; Tingting Deng; Hanqing Cai; Wan Zhang; Wenjian Zhang; Jinning Lou; Liang Peng
Source:
Life sciences 2019 v.225 pp. 107-116
ISSN:
0024-3205
Subject:
Western blotting; apoptosis; bovine serum albumin; cAMP-dependent protein kinase; caffeic acid; caspase-3; diabetic neuropathy; glycation; interleukins; nerve tissue; prostaglandin synthase; prostaglandins; rodents; secretion
Abstract:
... To explore the effect and mechanism of 1, 25-(OH)₂D₃ on Schwann cell apoptosis induced by advanced glycation end products. Schwann cells, isolated from rodent sciatic nerve were incubated with AGE-modified bovine serum albumin(AGE) to mimic diabetic conditions and 1,25-(OH)₂D₃ was used as protector. Cell apoptosis was detected by PI/Annexin-V staining, caspase 3 activity assay and western blotting ...
DOI:
10.1016/j.lfs.2019.03.068

24. 1,3-Dichloro-2-propanol inhibits progesterone production through the expression of steroidogenic enzymes and cAMP concentration in Leydig cells

Author:
Jianxia Sun; Shun Bai; Weibin Bai; Feiyan Zou; Lei Zhang; Guoqiang Li; Yunfeng Hu; Mingwei Li; Rian Yan; Zhijian Su; Yadong Huang
Source:
Food chemistry 2014 v.154 pp. 330-336
ISSN:
0308-8146
Subject:
Leydig cells; Western blotting; apoptosis; atomic force microscopy; cell viability; cyclic AMP; cytochrome P-450; enzymes; food processing; gels; gene expression; gene expression regulation; males; mechanism of action; membrane potential; messenger RNA; mitochondrial membrane; models; progesterone; quantitative polymerase chain reaction; radioimmunoassays; regulatory proteins
Abstract:
... 1,3-Dichloro-2-propanol (1,3-DCP) is a well-known food processing contaminant that has been shown to impede male reproductive function. However, its mechanism of action remains elusive. In this study, the effects of 1,3-DCP on progesterone production were investigated using the R2C Leydig cell model. 1,3-DCP significantly reduced cell viability from 7.48% to 97.4% at doses comprised between 0.5 an ...
DOI:
10.1016/j.foodchem.2014.01.017

25. 1,8-Cineole promotes G0/G1 cell cycle arrest and oxidative stress-induced senescence in HepG2 cells and sensitizes cells to anti-senescence drugs

Author:
Boris Rodenak-Kladniew; Agustina Castro; Peter Stärkel; Marianela Galle; Rosana Crespo
Source:
Life sciences 2020 v.243 pp. 117271
ISSN:
0024-3205
Subject:
Eucalyptus; Western blotting; acetylcysteine; adjuvants; antineoplastic agents; apoptosis; caspase-3; cell cycle checkpoints; cell growth; cell proliferation; cell senescence; cell viability; cineole; enzyme activity; essential oils; flow cytometry; fluorescence; fluorescence microscopy; hepatoma; mechanism of action; membrane potential; mitochondrial membrane; mitogen-activated protein kinase; oxidative stress; quercetin; reactive oxygen species; therapeutics; toxicity testing; vitamins
Abstract:
... 1,8-Cineole is a plant-derived monoterpene and a major constituent of Eucalyptus essential oil. Previously, we demonstrated that 1,8-cineole inhibited hepatocellular carcinoma (HCC) HepG2 cell growth. However, the underlying mechanisms remain unknown. Here, we evaluated the mechanisms of action of 1,8-cineole and the potential benefits of its combination with anticancer compounds harboring “anti-s ...
DOI:
10.1016/j.lfs.2020.117271

26. 1-(3′,5′-dihydroxyphenoxy)-7-(2″,4″,6-trihydroxyphenoxy)-2,4,9-trihydroxydibenzo-1,4-dioxin Inhibits Adipocyte Differentiation of 3T3-L1 Fibroblasts

Author:
Kong, Chang-Suk; Kim, Jung-Ae; Ahn, Byul-Nim; Vo, Thanh Sang; Yoon, Na-Young; Kim, Se-Kwon
Source:
Marine biotechnology 2010 v.12 no.3 pp. 299-307
ISSN:
1436-2228
Subject:
Ecklonia; Western blotting; adipocytes; adipogenesis; dose response; fatty acid-binding proteins; fatty acids; fatty-acid synthase; fibroblasts; gene expression regulation; genes; leptin; lipogenesis; lipolysis; lipoprotein lipase; long-chain-fatty-acid-CoA ligase; obesity; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; transport proteins; triacylglycerols; tumor necrosis factor-alpha
Abstract:
... In this study, we isolated the phloroglucinol derivative, 1-(3′,5′-dihydroxyphenoxy)-7-(2″,4″,6-trihydroxyphenoxy)-2,4,9-trihydroxydibenzo-1,4-dioxin (1), from Ecklonia cava and evaluated its potential inhibition on adipocyte differentiation in 3T3-L1 cells. Lipid accumulation along with the expression of several genes associated with adipogenesis and lipolysis was examined at the end of different ...
DOI:
10.1007/s10126-009-9224-z
PubMed:
19680725

27. IL- 10 derived from Hepatocarcinoma cells improves human induced regulatory T cells function via JAK1/STAT5 pathway in tumor microenvironment

Author:
Shaopeng Zhang; Xiaojie Gan; Jiannan Qiu; Zheng Ju; Ji Gao; Jinren Zhou; Chengyu Shi; Yaqing Zhu; Zhang Li
Source:
Molecular immunology 2021 v.133 pp. 163-172
ISSN:
0161-5890
Subject:
T-lymphocytes; Western blotting; antibodies; antineoplastic activity; apoptosis; coculture; decline; hepatocytes; hepatoma; homeostasis; humans; immune response; immunotherapy; interleukin-10; phosphorylation
Abstract:
... Forkhead box P3 (Foxp3) expressing CD4⁺CD25⁺ regulatory T cells (Tregs), an essential subset of immune T cells for maintaining immune homeostasis is implicated as a negative regulator in an anti-tumor immune response. Current researches suggest that reducing tumor-infiltrating Tregs contribute to enhanced anti-cancer effect. However, the mechanism of infiltration of a large number of Tregs into tu ...
DOI:
10.1016/j.molimm.2021.02.014

28. A 10-kDa class-Cl sHsp protects E. coli from oxidative and high-temperature stress

Author:
Jofre, A.; Molinas, M.; Pla, M.
Source:
Planta 2003 v.217 no.5 pp. 813-819
ISSN:
0032-0935
Subject:
cork; heat shock proteins; forest trees; transgenes; complementary DNA; oxidative stress; immunoassays; sequence alignment; Escherichia coli; heat stress; amino acid sequences; Western blotting; nucleotide sequences; molecular cloning; Quercus suber; crystallins; plant proteins
DOI:
10.1007/s00425-003-1048-x

29. 103 Characterization of the receptors of the endocannabinoid system in equine sperm: Possible role of anandamide in sperm function

Author:
C. Arroyo-Salvo; R. Lottero; A. Gambini; S. Perez Martinez
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 159
ISSN:
1031-3613
Subject:
Western blotting; air; cAMP-dependent protein kinase; cannabinoids; cattle; cryopreservation; flagellum; fluorescence; fluorescent antibody technique; glucose; mice; polyvinyl alcohol; sperm capacitation; spermatozoa; stallions; transient receptor potential vanilloid channels; zona pellucida
Abstract:
... Conventional IVF in horses remains challenging. In particular, stallion sperm fails to penetrate the zona pellucida, possibly due to incomplete invitro sperm capacitation. Therefore, there is a need to elucidate, in horses, molecules with a proven role during capacitation in other mammals. Our laboratory has described the relevance of the endocannabinoid system in capacitation of bovine and murine ...
DOI:
10.1071/RDv33n2Ab103

30. 105 Akt signaling mediates prostate cancer response to cryoablation

Author:
John M. Baust; Daniel Klossner; Andrew Gage; Robert Van Buskirk; John G. Baust
Source:
Cryobiology 2013 v.67 no.3 pp. 427-428
ISSN:
0011-2240
Subject:
Western blotting; androgen receptors; apoptosis; cell lines; cell viability; cryosurgery; freezing; men; mitogen-activated protein kinase; patients; prostatic neoplasms; quality of life; remission; signal transduction; United States
Abstract:
... Prostate cancer (CaP) has a significant impact on men’s health and quality of life with more than 230,000 new cases diagnosed annually in the United States. The androgen signaling pathway is integral in CaP progression and as such androgen deprivation is a widely utilized therapeutic option to treat CaP. Typically, with the application of the anti androgen treatment, the androgen sensitive (AS) Ca ...
DOI:
10.1016/j.cryobiol.2013.09.111

31. 11-Ketotestosterone induces oocyte growth, but does not affect oocyte cytology in pre-vitellogenic captive beluga, Huso huso L

Author:
Sobhan R. Akhavan; Bahram Falahatkar; Joanna M. Ward; P. Mark Lokman
Source:
Comparative biochemistry and physiology 2019 v.232 pp. 51-59
ISSN:
1096-4959
Subject:
Gadus morhua; Huso huso; Western blotting; apolipoprotein E; biopsy; blood serum; cholesterol; eel; gene expression; genes; histology; hydrolysis; lipoprotein lipase; low density lipoprotein; low density lipoprotein receptors; messenger RNA; oocytes; ovarian development; salmon; triacylglycerols; very low density lipoprotein
Abstract:
... An effect of 11-ketotestosterone (11-KT) on growth of previtellogenic (PV) ovaries of eel, salmon and Atlantic cod has been demonstrated. The purpose of this study was to investigate the effects of 11-KT treatment (in vivo) on ovarian growth, on hormonal and biochemical changes in blood, and on ovarian mRNA levels of lipidation-related genes in captive beluga with PV oocytes. In addition, the pote ...
DOI:
10.1016/j.cbpb.2019.02.009

32. 117 development of a noninvasive, fecal protein pregnancy test for polar bears

Author:
C. DeLorenzo; B. Lynch; T. Roth; K. Petren; E. Curry
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 188-189
ISSN:
1031-3613
Subject:
Ursus maritimus; Western blotting; ammonium sulfate; autumn; biomarkers; feces; females; fetal development; humans; polyclonal antibodies; prealbumin; pregnancy; progesterone; protein content; pseudopregnancy; spring; summer; thyroid hormones
Abstract:
... Differentiating between pregnancy and nonpregnancy noninvasively is difficult in species that experience pseudopregnancy, including polar bears (Ursus maritimus). These bears usually breed in the spring, undergo delayed implantation until late summer or early fall, and give birth in late fall. In other species, the placental protein transthyretin (TTR) has been shown to be essential for early feta ...
DOI:
10.1071/RDv28n2Ab116

33. 12-Deoxyphorbol 13-palmitate inhibit VEGF-induced angiogenesis via suppression of VEGFR-2-signaling pathway

Author:
Hui-Yu Xu; Yuan-Ming Pan; Zhi-Wei Chen; Yu Lin; Li-Hong Wang; Yi-Hua Chen; Ting-Ting Jie; You-Yong Lu; Ji-Cheng Liu
Source:
Journal of ethnopharmacology 2013 v.146 no.3 pp. 724-733
ISSN:
0378-8741
Subject:
Euphorbia; Western blotting; angiogenesis; animal models; chorioallantoic membrane; endothelial cells; fibroblast growth factor 2; gelatinase A; gene expression regulation; immunohistochemistry; metastasis; mice; protein synthesis; proteins; spices; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: 12-Deoxyphorbol 13-palmitate (G) is one toxic compound isolated from Euphorbia fischeriana, an Asian spice used for cancer treatment as a folk remedy. However, whether 12-deoxyphorbol 13-palmitate affects angiogenesis remains unclear. AIM OF THE STUDY: To explore the in vitro and in vivo antiangiogenic effects of 12-deoxyphorbol 13-palmitate and its underlying mecha ...
DOI:
10.1016/j.jep.2013.01.007
PubMed:
23434607

34. 127 Temporal regulation of molecular pathways, metabolic enzymes and growth factor receptors during bovine oocyte maturation in vitro

Author:
S. Rajput; J. Becker; Y. Yuan; W. Schoolcraft; R. Krisher
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 189
ISSN:
1031-3613
Subject:
Western blotting; analysis of variance; antibodies; assisted reproductive technologies; beta oxidation; cattle; dephosphorylation; fibroblast growth factor receptor 1; germinal vesicle; growth factors; metaphase; mitogen-activated protein kinase; phosphorylation; pyruvic acid; receptors; signal transduction; tricarboxylic acid cycle
Abstract:
... Although great efforts have been made to improve in vitro oocyte maturation (IVM) medium, we have yet to achieve competence equivalent to in vivo-matured oocytes. The failure in development of culture conditions for IVM yielding high quality eggs is attributed to an incomplete understanding of molecular pathways regulating oocyte and cumulus cell metabolism. The objective of the present study was ...
DOI:
10.1071/RDv31n1Ab127

35. 129 Knockdown of connexin 43 effects on developmental competence in porcine parthenotes

Author:
K.-T. Shin; Y.-J. Niu; Z.-W. Nie; W. Zhou; Y.-H. Kim; X.-S. Cui
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 190
ISSN:
1031-3613
Subject:
Western blotting; apoptosis; autophagy; blastocyst; caspase-3; connexins; diploidy; double-stranded RNA; early development; embryogenesis; fluorescein; gene expression regulation; genes; in vitro fertilization; membrane permeability; polyspermy; reactive oxygen species; somatic cells; swine; tight junctions; transmembrane proteins; zygote
Abstract:
... Connexin 43 (Cx43) is one of the gap junction proteins that are compounds of transmembrane proteins and transports the small-molecular-weight chemicals up to 2 kDa. Lacking of Cx43 influences the junctional protein and induces autophagy and apoptosis in somatic cells. However, the function of Cx43 in porcine early embryos is still unknown. With the aim to find out the molecular mechanism of Cx43 o ...
DOI:
10.1071/RDv31n1Ab129

36. IL- 13 regulates IL-17C expression by suppressing NF-κB-mediated transcriptional activation in airway epithelial cells

Author:
Katsumasa Yamanaka; Tomoyuki Fujisawa; Hideki Kusagaya; Kazutaka Mori; Mitsuru Niwa; Kazuki Furuhashi; Masato Kono; Etsuko Hamada; Takafumi Suda; Masato Maekawa
Source:
Biochemical and biophysical research communications 2018 v.495 no.1 pp. 1534-1540
ISSN:
0006-291X
Subject:
Western blotting; chromatin; epithelial cells; epithelium; humans; innate immunity; interleukin-13; interleukin-17; interleukin-1beta; messenger RNA; non-specific protein-tyrosine kinase; phosphorylation; precipitin tests; protein synthesis; small interfering RNA; transactivators; transcription (genetics); transcription factor NF-kappa B; transcriptional activation
Abstract:
... The cytokine interleukin (IL)-17C is highly expressed in epithelial tissues and involved in innate immune responses; however, the regulation of IL-17C expression in the airways remains poorly understood. Here, we show that IL-1β strongly induces both IL-17C mRNA and protein expression in primary normal human bronchial epithelial cells. Conversely, IL-13 significantly reduced the IL-1β-induced IL-1 ...
DOI:
10.1016/j.bbrc.2017.11.207

37. 13,13a-seco-protoberberines from the tubers of Corydalis yanhusuo and their anti-inflammatory activity

Author:
Gui-Yang Xia; Dong-Jie Fang; Ling-Yan Wang; Huan Xia; Ya-Nan Wang; Hong-Cai Shang; Sheng Lin
Source:
Phytochemistry 2022 v.194 pp. 113023
ISSN:
0031-9422
Subject:
Corydalis yanhusuo; Western blotting; active sites; anti-inflammatory activity; bioassays; enantiomers; inducible nitric oxide synthase; interleukin-6; lipopolysaccharides; molecular dynamics; nitric oxide; plant biochemistry; skeleton; spectral analysis; transcription factor NF-kappa B
Abstract:
... Six undescribed protoberberine derivatives including two pairs of enantiomers, named yanhusanines G-L, along with fifteen reported protoberberine alkaloids, were isolated from the tubers of Corydalis yanhusuo. Among them, yanhusanines H-L feature a unique 13,13a-seco skeleton which is rare in nature. Their structural elucidations were achieved by extensive spectroscopic analysis and quantum chemis ...
DOI:
10.1016/j.phytochem.2021.113023

38. 13-Acetoxysarcocrassolide Exhibits Cytotoxic Activity against Oral Cancer Cells through the Interruption of the Keap1/Nrf2/p62/SQSTM1 Pathway: The Need to Move Beyond Classical Concepts

Author:
Yi-Chang Liu; Bo-Rong Peng; Kai-Cheng Hsu; Mohamed El-Shazly; Shou-Ping Shih; Tony Eight Lin; Fu-Wen Kuo; Yi-Cheng Chou; Hung-Yu Lin; Mei-Chin Lu
Source:
Marine drugs 2020 v.18 no.8 pp. -
ISSN:
1660-3397
Subject:
DNA; Western blotting; acetylcysteine; animal models; antineoplastic activity; antineoplastic agents; antioxidant enzymes; apoptosis; cell viability; corals; cytotoxicity; enzyme activity; flow cytometry; fluorescent antibody technique; immunostimulants; mechanism of action; membrane potential; mitochondrial membrane; mouth neoplasms; neoplasm cells; precipitin tests; proteins; reactive oxygen species; small interfering RNA; toxicity testing; transfection; xenotransplantation
Abstract:
... 13-Acetoxysarcocrassolide (13-AC), a marine cytotoxic product isolated from the alcyonacean coral Lobophytum crassum, exhibited potent antitumor and immunostimulant effects as reported in previous studies. However, the 13-AC antitumor mechanism of action against oral cancer cells remains unclear. The activity of 13-AC against Ca9-22 cancer cells was determined using MTT assay, flow cytometric anal ...
DOI:
10.3390/md18080382

39. 14-Deoxy-11,12-didehydroandrographolide inhibits proliferation and induces GSH-dependent cell death of human promonocytic leukemic cells

Author:
Rahul Raghavan; Sanith Cheriyamundath; Joseph Madassery
Source:
Natural medicines 2014 v.68 no.2 pp. 387-394
ISSN:
1340-3443
Subject:
Andrographis paniculata; DNA; Western blotting; apoptosis; caspase-3; cell viability; cytotoxicity; glutathione; humans; inhibitory concentration 50; leukemia
Abstract:
... 14-Deoxy-11,12-didehydroandrographolide (AND2), an analogue of andrographolide, showed more potent cytotoxicity against human promonocytic leukemia (THP-1) cells than adherent cancer cell lines. In this study AND2 was isolated from the plant Andrographis paniculata and it was characterized. The antiproliferative effect of AND2 on both adherent (PC-3 and MDAMB) and non-adherent (THP-1 and Jurkat) c ...
DOI:
10.1007/s11418-014-0815-2
PubMed:
24458985

40. 145 ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study

Author:
I. Ruelle; N. Seregeant; D. Bencharif; F. Charreaux; C. Thorin; S. Michaud; M. Dordas-Perpinyà; N. Jouy; S. Audry; C. Maurage; M. Delehedde; L. Briand-Amirat
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 199
ISSN:
1031-3613
Subject:
Holstein; Western blotting; animal models; antibodies; bulls; embryo (animal); enzyme-linked immunosorbent assay; flow cytometry; human reproduction; in vitro fertilization; insemination; knockout mutants; male fertility; mice; microscopy; semen; sperm quality; spermatids; spermatogenesis; straw; testes
Abstract:
... Recently, ProAKAP4 has been described as a pertinent indicator of sperm quality in humans, pigs, and stallions. In knockout mouse models lacking AKAP4 expression, the male mice were infertile. As high proAKAP4 levels were significantly correlated with a lower proportion of abortions in intrauterine insemination settings in human reproduction, proAKAP4 could be considered a pertinent new sperm para ...
DOI:
10.1071/RDv32n2Ab145

41. IL- 15 up-regulates the MMP-9 expression levels and induces inflammatory infiltration of macrophages in polymyositis through regulating the NF-kB pathway

Author:
Wang Yan; Weinv Fan; Caijing Chen; Yunqin Wu; Zhenyi Fan; Jiaqi Chen; Zhaoying Chen; Huimin Chen
Source:
Gene 2016 v.591 no.1 pp. 137-147
ISSN:
0378-1119
Subject:
Western blotting; blood; cell movement; creatine kinase; enzyme-linked immunosorbent assay; immunohistochemistry; interleukin-15; macrophages; mitogen-activated protein kinase; patients; polymyositis; rats; small interfering RNA; transcription factor NF-kappa B
Abstract:
... This study was aimed to research the effects of IL-15 on inducing inflammatory infiltration of macrophages in polymyositis (PM) through the NF-kB pathway, and whether IL-15 was able to further regulate MMP-9 expression levels. Prepared PM cells, collected from the patients suffering from PM, were administered to SD rats. Also, a group of healthy SD rats was undergoing the same treatment as the con ...
DOI:
10.1016/j.gene.2016.06.055

42. 15-Hydroxy-8(17),13(E)-labdadiene-19-carboxylic acid (HLCA) inhibits proliferation and induces cell cycle arrest and apoptosis in ovarian cancer cells

Author:
Hadi Bakhtiari; Ali Gheysarzadeh; Mustafa Ghanadian; Mahmoud Aghaei
Source:
Life sciences 2021 v.267 pp. 118981
ISSN:
0024-3205
Subject:
Juniperus; Western blotting; antineoplastic agents; apoptosis; caspase-9; cell cycle checkpoints; cyclin-dependent kinase; cyclins; cytotoxicity; flow cytometry; interphase; ovarian neoplasms; reactive oxygen species; therapeutics
Abstract:
... 15-Hydroxy-8(17),13(E)-labdadiene-19-carboxylic acid (HLCA) isolated from Juniperus foetidissima, has been recently identified as an antiproliferative agent; however, the molecular basis of antiproliferative effects of HLCA remains unknown. To investigate it, the current study has emphasized the hypothesis that HLCA induced cell death is a consequence of intracellular reactive oxygen species (ROS) ...
DOI:
10.1016/j.lfs.2020.118981

43. 15-Lipoxygenase is a component of the mammalian sperm cytoplasmic droplet

Author:
Fischer, K A; Van Leyen, K; Lovercamp, K W; Manandhar, G; Sutovsky, M; Feng, D; Safranski, T; Sutovsky, P
Source:
Reproduction 2005 v.130 no.2 pp. 213-222
ISSN:
1470-1626
Subject:
Western blotting; antiserum; boars; fluorescent antibody technique; humans; mice; mitochondria; phospholipids; proteolysis; rabbits; reticulocytes; seminal plasma; spermatozoa; spermiogenesis; stallions; testes; ubiquitin; ubiquitin-protein ligase
Abstract:
... Lipoxygenases (LOXs) are a family of enzymes capable of peroxidizing phospholipids. A member of the LOX family of enzymes, 15-LOX, participates in the degradation of mitochondria and other organelles within differentiating red blood cells, the reticulocytes. The present study provides biochemical and immunocytochemical evidence for the presence of 15-LOX in the sperm cytoplasmic droplet (CD). Test ...
DOI:
10.1530/rep.1.00646
PubMed:
16049159

44. 16-Hydroxycleroda-3, 13-dien-15, 16-olide inhibits the proliferation and induces mitochondrial-dependent apoptosis through Akt, mTOR, and MEK-ERK pathways in human renal carcinoma cells

Author:
Cheng Liu; Wei-Chang Lee; Bu-Miin Huang; Yi-Chen Chia; Yu-Chi Chen; Yung-Chia Chen
Source:
Phytomedicine 2017 v.36 pp. 95-107
ISSN:
0944-7113
Subject:
B-cell lymphoma; NAD ADP-ribosyltransferase; Polyalthia longifolia; Western blotting; acridine orange; antineoplastic activity; apoptosis; caspase-3; cell proliferation; cytochrome c; drug therapy; ethidium; fluorescent dyes; heat-shock protein 70; humans; hypoxia; iodides; membrane potential; mitochondria; mitochondrial membrane; mitogen-activated protein kinase; phosphorylation; pro-apoptotic proteins; radiotherapy; reactive oxygen species; renal cell carcinoma; staining
Abstract:
... Renal cell carcinoma (RCC) is well known that it cannot be treated with traditional chemotherapy or radiotherapy. 16-Hydroxycleroda-3,13-dien-15,16-olide (CD), isolated from Polyalthia longifolia Benth. & Hook. f. var. pendula had been reported to display significant efficacy against cancer cell lines.To determine the anti-tumour activities of CD in two clear cell type RCC (ccRCC) cell lines (A-49 ...
DOI:
10.1016/j.phymed.2017.09.021

45. 16-Hydroxycleroda-3,13-dien-15,16-olide regulates the expression of histone-modifying enzymes PRC2 complex and induces apoptosis in CML K562 cells

Author:
Yi-Hsiung Lin; Chien-Chih Lee; Fang-Rong Chang; Wen-Hsin Chang; Yang-Chang Wu; Jan-Gowth Chang
Source:
Life sciences 2011 v.89 no.23-24 pp. 886-895
ISSN:
0024-3205
Subject:
quantitative polymerase chain reaction; epigenetics; protein synthesis; cell cycle; drug therapy; leukemia; histones; enzymes; tumor suppressor genes; Western blotting; gene expression; gene silencing; cell proliferation; fluorescent antibody technique; apoptosis
Abstract:
... AIMS: Histone modifications play central epigenetic roles in regulating the entire genome of the cell and cell proliferation. Herein, we investigated the effects of the natural compound, 16-hydroxycleroda-3,13-dien-15,16-olide (PL3), on the expressions of histone-modifying enzymes, and examined how it induces apoptosis in leukemia K562 cells. MAIN METHODS: Cell proliferation was determined by an M ...
DOI:
10.1016/j.lfs.2011.09.011

46. 17-AAG, a Hsp90 inhibitor, attenuates the hypoxia-induced expression of SDF-1α and ILK in mouse RPE cells

Author:
Wang, Ye Qing; Zhang, Xiao Mei; Wang, Xiao Dan; Wang, Bin Jie; Wang, Wei
Source:
Molecular biology reports 2010 v.37 no.3 pp. 1203-1209
ISSN:
0301-4851
Subject:
Western blotting; cobalt; cultured cells; epithelium; heat shock proteins; hypoxia; mice; reverse transcriptase polymerase chain reaction
Abstract:
... The aim of this study was to investigate the changes of SDF-1α and ILK expression in mouse retinal pigment epithelium (RPE) cells in response to hypoxia, and the effect of 17-Allylamino-17-demethoxygeldanamycin (17-AAG), a heat shock protein 90 (Hsp90) inhibitor, on the hypoxia-induced expression of SDF-1α and ILK. RPE cells were cultured with 200 μmol/L cobalt chloride (CoCl₂) for different times ...
DOI:
10.1007/s11033-009-9490-x

47. A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein

Author:
Emma Herrera; Patricia Barcenas; Rubicela Hernández; Alfonso Méndez; Guillermo Pérez-Ishiwara; Blanca Barrón
Source:
Virology journal 2010 v.7 no.1 pp. 195
ISSN:
1743-422X
Subject:
Mumps orthorubulavirus; RNA; Western blotting; amino acids; antiserum; blood serum; cytokines; fluorescent antibody technique; genes; genetic vectors; hamsters; hemagglutination; immunization; immunochemistry; neutralization; plasmids; polypeptides; rabbits; sialidase; virology
Abstract:
... BACKGROUND: The hemagglutinin-neuraminidase (HN) protein is the major antigenic determinant of the Mumps virus (MuV) and plays an important role in the viral infectious cycle through its hemagglutination/hemadsorption (HA/HD) and neuraminidase (NA) activities. Objective: analyze the biological and immunological properties of a polypeptide derived from a highly conserved region of the HN ectodomain ...
DOI:
10.1186/1743-422X-7-195

48. IL- 17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein

Author:
Ling Chen; Sheng Guo; Liangxia Wu; Xiaoyong Fan; Hui Ma; Kang Wu; Juan Wu; Jianhua Zhang
Source:
Applied biochemistry and biotechnology 2015 v.176 no.7 pp. 2018-2026
ISSN:
0273-2289
Subject:
Mycobacterium smegmatis; Western blotting; asthma; autoantibodies; autoimmune diseases; electroporation; enzyme-linked immunosorbent assay; gene fusion; genetic vectors; immune response; interleukin-17; mice; plasmids
Abstract:
... Interleukin-17A is a newly described proinflammatory cytokine, which plays important roles in autoimmune diseases as well as asthma. In current work, we constructed a recombinant plasmid pMFA42S-Ag85a-IL-17a by inserting fusion gene Ag85a-IL-17a into shuttle vector pMFA42S, which was transformed to Mycobacterium smegmatis by electroporation to obtain recombinant M. smegmatis named rMS-Ag85a-IL-17a ...
DOI:
10.1007/s12010-015-1697-9

49. IL- 17A induces chromatin remodeling promoting IL-8 release in bronchial epithelial cells: Effect of Tiotropium

Author:
Giulia Anzalone; Rosalia Gagliardo; Fabio Bucchieri; Giusy Daniela Albano; Liboria Siena; Angela Marina Montalbano; Anna Bonanno; Loredana Riccobono; Michael Paul Pieper; Mark Gjomarkaj; Mirella Profita
Source:
Life sciences 2016 v.152 pp. 107-116
ISSN:
0024-3205
Subject:
Western blotting; anti-inflammatory activity; chromatin; enzyme-linked immunosorbent assay; epithelial cells; epithelium; histone deacetylase; histones; human cell lines; humans; inflammation; interleukin-17; interleukin-8; messenger RNA; oxidative stress; patients; quantitative polymerase chain reaction
Abstract:
... IL-17A plays a key role in the persistence of airway inflammation, oxidative stress, and reduction of steroid-sensitivity in COPD. We studied the effect of IL-17A on chromatin remodeling and IL-8 production.We measured the levels of IL-8 and IL-17A in induced sputum supernatants (ISS) from healthy controls (HCs), healthy smokers (HSs), and COPD patients by enzyme-linked immunosorbent assay (ELISA) ...
DOI:
10.1016/j.lfs.2016.03.031

50. IL- 17A suppresses and curcumin up-regulates Akt expression upon bleomycin exposure

Author:
Mahesh Manjunath Gouda; Ashwini Prabhu; Yashodhar Prabhakar Bhandary
Source:
Molecular biology reports 2018 v.45 no.4 pp. 645-650
ISSN:
0301-4851
Subject:
Western blotting; cell proliferation; curcumin; epithelial cells; inflammation; interleukin-17; lungs; phosphorylation; therapeutics
Abstract:
... Pro-inflammatory cytokine IL-17A modulates the expression of Akt in bleomycin (BLM) administered alveolar basal epithelial cells, the mechanism behind which remains unclear. This investigation was carried out to assess IL-17A mediated down-regulation of Akt expression and the pivotal role of curcumin as a regulatory molecule. Alveolar basal epithelial cells were treated with BLM and IL-17A and cur ...
DOI:
10.1007/s11033-018-4199-3

51. IL- 17A-stimulated endothelial fatty acid β-oxidation promotes tumor angiogenesis

Author:
Ruirui Wang; Xiaohan Lou; Guang Feng; Jinfeng Chen; Linyu Zhu; Xiaomeng Liu; Xiaohan Yao; Pan Li; Jiajia Wan; Yi Zhang; Chen Ni; Zhihai Qin
Source:
Life sciences 2019 v.229 pp. 46-56
ISSN:
0024-3205
Subject:
AMP-activated protein kinase; Western blotting; angiogenesis; beta oxidation; fatty acids; human cell lines; human umbilical vein endothelial cells; humans; interleukin-17; liquid chromatography; lung neoplasms; mass spectrometry; mitochondria; models; neoplasm cells; signal transduction; therapeutics
Abstract:
... Tumor growth is an angiogenesis-dependent process that requires sustained new vessel growth. Interleukin-17 (IL-17A) is a key cytokine that modulates tumor progression. However, whether IL-17A affects the metabolism of endothelial cells is unknown.A xenograft model was established by implanting H460 (human lung cancer cell line) cells transfected with IL-17A-expressing or control vector. The effec ...
DOI:
10.1016/j.lfs.2019.05.030

52. IL- 17RB enhances thyroid cancer cell invasion and metastasis via ERK1/2 pathway-mediated MMP-9 expression

Author:
Lei Ren; Yapei Xu; Cong Liu; Shoujun Wang; Guijun Qin
Source:
Molecular Immunology 2017 pp. -
ISSN:
0161-5890
Subject:
in vivo studies; quantitative polymerase chain reaction; interleukin-17; therapeutics; Western blotting; metastasis; neoplasm cells; tissues; thyroid neoplasms
Abstract:
... IL-17RB, a member of the IL-17 receptor family that can be activated by IL-17B, has been proved to be involved in inflammatory diseases and cancers. However, the function of IL-17RB in thyroid cancer is still unknown. In this study, IL-17RB expression in thyroid cancer cell lines and tissues was examined by real-time PCR and western blot. The effects of IL-17RB on cell invasion and migration were ...
DOI:
10.1016/j.molimm.2017.06.034

53. 17β-Estradiol Regulates SKP2 Expression in Cultured Immature Boar Sertoli Cells Mainly via Estrogen Receptor β, cAMP-PKA and ERK1/2

Author:
Xian-zhong WANG; Feng-wei ZHU; Yong WANG; Yi WANG; Jiao-jiao ZHANG; Jia-hua ZHANG
Source:
Journal of integrative agriculture 2014 v.13 no.4 pp. 827-836
ISSN:
2095-3119
Subject:
Sertoli cells; Western blotting; antagonists; boars; cAMP-dependent protein kinase; cell cycle; correlation; cyclic AMP; estradiol; estrogen receptors; forskolin; messenger RNA; mitogen-activated protein kinase; proliferating cell nuclear antigen; protein synthesis; quantitative polymerase chain reaction
Abstract:
... Estrogen plays an important role in regulating testicular Sertoli cell number. Furthermore, S-phase kinase-associated protein 2 (SKP2) plays a central role in mammalian cell cycle progression. The objective of this study was to determine whether 17β-estradiol can regulate the expression of SKP2, and the Sertoli cell cycle, via estrogen receptor β (ERβ), the cyclic adenosine monophosphate (cAMP)-pr ...
DOI:
10.1016/S2095-3119(13)60430-X

54. 17β-Estradiol and progesterone decrease MDP induced NOD2 expression in bovine mammary epithelial cells

Author:
Dan-dan Xu; Gang Wang; Xian-jing He; Jian-fa Wang; Bin Yang; Zhi-peng Sun; Dong-bo Sun; Qian-yu He; Xu Zhang; Rui Wu
Source:
Veterinary immunology and immunopathology 2017 v.188 pp. 59-64
ISSN:
0165-2427
Subject:
Western blotting; animal tissues; chemical elements; dairy cows; epithelial cells; estradiol; humans; innate immunity; interleukin-6; interleukin-8; intracellular signaling peptides and proteins; laboratory animals; mammary glands; mastitis; messenger RNA; oligomerization; perinatal period; progesterone; protein kinases; protein synthesis; quantitative polymerase chain reaction; receptors; reverse transcriptase polymerase chain reaction; transcription (genetics); tumor necrosis factor-alpha
Abstract:
... During the periparturient period, many neuroendocrine changes develop in cows. Periparturient hormone fluxes may adversely affect mammary gland immunity and mastitis susceptibility. 17β-Estradiol (E2) and progesterone (P4) have been reported to function on immune regulation, and their concentration fluctuates dramatically during the perinatal period. Nucleotide-binding oligomerization domain (NOD) ...
DOI:
10.1016/j.vetimm.2017.04.010

55. 17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner

Author:
Ana Laura Pereira-Suárez; María de la Luz Galván-Ramírez; Laura Rocío Rodríguez-Pérez; Edgar I. López-Pulido; Christian David Hernández-Silva; Inocencia Guadalupe Ramírez-López; Grecia Viridiana Morales Amaya; Luis David Lopez Cabrera; José Francisco Muñoz-Valle; Adrián Ramírez-de-Arellano
Source:
Molecular and biochemical parasitology 2022 v.247 pp. 111433
ISSN:
0166-6851
Subject:
Toxoplasma gondii; Western blotting; cell growth; estrogen receptors; inflammation; macrophages; models; monocytes; parasites; parasitology; phosphatidylinositol 3-kinase; pregnancy; prolactin receptors; tachyzoites
Abstract:
... Toxoplasma gondii (T. gondii) is a parasite common in pregnancy. Monocytes and macrophages are a significant immunologic barrier against T. gondii by boosting up inflammation. This outcome is highly regulated by signaling pathways such as MAPK (ERK1/2) and PI3K (AKT), necessary in cell growth and proliferation. It may be associated with the hormonal receptors’ modulation by T. gondii (Estrogen Rec ...
DOI:
10.1016/j.molbiopara.2021.111433

56. 17β-estradiol protects nucleus pulposus cells from serum deprivation-induced apoptosis and regulates expression of MMP-3 and MMP-13 through promotion of autophagy

Author:
Peng Ao; Wenzhou Huang; Jian Li; Tianlong Wu; Libiao Xu; Zhongbo Deng; Wenjie Chen; Changchang Yin; Xigao Cheng
Source:
Biochemical and biophysical research communications 2018 v.503 no.2 pp. 791-797
ISSN:
0006-291X
Subject:
Western blotting; apoptosis; autophagy; blood serum; estradiol; flow cytometry; homeostasis; intervertebral disks; metalloproteinases; protective effect; proteins; rats; transfection
Abstract:
... Serum deprivation is a likely contributor to intervertebral disc (IVD) degeneration (IVDD).17β-estradiol (E2) have been noted to protect nucleus pulposus cells (NPCs) against apoptosis. Autophagy and apoptosis play a paramount role in maintaining the homeostasis of IVD. So far, little research has been published on whether autophagy plays a role for the E2 mediated protection of NPCs. The aim of t ...
DOI:
10.1016/j.bbrc.2018.06.077

57. 17β-estradiol reduces NF-κB expression induced by increased crosstalk between KLF5 and ERα in murine vascular smooth muscle cells

Author:
Man-li Zhang; Man-na Zhang; Wen-li Wang; Hui Chen; Xia Wang; Xuan Li; Li Li; Fei Tong
Source:
Biochemical and biophysical research communications 2022 v.625 pp. 181-187
ISSN:
0006-291X
Subject:
Western blotting; cardiovascular system; cell proliferation; chromatin immunoprecipitation; fluorescent antibody technique; inflammation; luciferase; research; smooth muscle
Abstract:
... Pathological vascular remodeling and cell proliferation play vital roles in many proliferative vascular diseases. Estrogen can protect the cardiovascular system, but its exact molecular mechanism is unknown. Here we report that 17β-estradiol (E2) suppressed vascular smooth muscle cells (VSMCs) proliferation and inflammation. qRT-PCR and Western blot demonstrated that E2 decreased NF-κB p50 express ...
DOI:
10.1016/j.bbrc.2022.07.054

58. 180 Stallion sperm phospholipase C zeta affects cleavage rates after intracytoplasmic injection in bovine oocytes

Author:
F. Amoroso-Sanches; R. Gonzalez-Castro; J. Stokes; E. Carnevale
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 214-215
ISSN:
1031-3613
Subject:
DNA; Western blotting; air; animal ovaries; antibodies; carbon dioxide; cattle; chromatin; embryo (animal); embryo culture; embryogenesis; flow cytometry; fluorescence; fluorescent antibody technique; freeze-thaw cycles; humans; intracytoplasmic sperm injection; liquids; nitrogen; oocytes; parthenogenesis; phospholipase C; spermatozoa; stallions; straw; viability; zygote
Abstract:
... Phospholipase C zeta (PLCz) is a sperm protein linked to oocyte activation and zygote development in diverse species. Human intracytoplasmic sperm injection (ICSI) success is poor when sperm PLCz is reduced or mutated. We hypothesised that the expression of PLCz in stallion sperm corresponds with cleavage rates after ICSI. For this study, we selected sperm from 4 of 21 stallions for which frozen-t ...
DOI:
10.1071/RDv31n1Ab180

59. 182 Bisphenol A, but not bisphenol S, affects key microRNAs during bovine oocyte maturation

Author:
R. Sabry; M. Nguyen; L. Stalker; J. LaMarre; L. Favetta
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 219
ISSN:
1031-3613
Subject:
Western blotting; acrylamides; actin; adverse effects; analysis of variance; animal reproduction; bisphenol A; bisphenol S; cattle; complementary DNA; densitometry; endocrine-disrupting chemicals; epigenetics; essential genes; females; gels; gene expression; mechanism of action; messenger RNA; microRNA; oocytes; protein content; quantitative polymerase chain reaction; sonication; toxicity; transcription (genetics)
Abstract:
... Oocyte maturation involves crucial hormone-dependent events that are uniquely susceptible to toxic insults by endocrine disrupting chemicals (EDCs). Emerging evidence suggests that small RNAs, including microRNAs (miRNAs), may be key participants in the response to EDCs. Bisphenol A (BPA) and bisphenol S (BPS) are chemicals with detrimental health effects, with BPA negatively affecting oocyte qual ...
DOI:
10.1071/RDv32n2Ab182

60. 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model

Author:
Mojtaba Malekinejad; Mohammad Reza Pashaee; Hassan Malekinejad
Source:
European journal of nutrition 2022 v.61 no.7 pp. 3437-3447
ISSN:
1436-6207
Subject:
Western blotting; absorption; adhesion; biochemical pathways; cell viability; cytotoxicity; dietary supplements; electrical resistance; epithelium; glycyrrhizin; human cell lines; humans; intestines; luciferase; metabolites; models; occludins; permeability; protein content; tight junctions; toxicity testing; traditional medicine
Abstract:
... PURPOSE: Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated. METHODS: The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity usin ...
DOI:
10.1007/s00394-022-02900-4

61. 18β-Glycyrrhetinic acid inhibits adipogenic differentiation and stimulates lipolysis

Author:
Myung-Hee Moon; Jae-Kyo Jeong; You-Jin Lee; Jae-Won Seol; Dong-Choon Ahn; In-Shik Kim; Sang-Youel Park
Source:
Biochemical and biophysical research communications 2012 v.420 no.4 pp. 805-810
ISSN:
0006-291X
Subject:
Western blotting; adipocytes; adipogenesis; adiponectin; anti-inflammatory activity; antibacterial properties; enzymes; gene expression regulation; glycerol; insulin; licorice; lipolysis; messenger RNA; mice; models; obesity; phosphorylation; reverse transcriptase polymerase chain reaction; serine; transcription (genetics); transcription factors
Abstract:
... 18β-Glycyrrhetinic acid (18β-GA) obtained from the herb liquorice has various pharmacological properties including anti-inflammatory and anti-bacterial activities. However, potential biological anti-obesity activities are unclear. In this study, novel biological activities of 18β-GA in the adipogenesis of 3T3-L1 preadipocytes and in lipolysis of differentiated adipocytes were identified. Mouse 3T3 ...
DOI:
10.1016/j.bbrc.2012.03.078

62. 18β-Glycyrrhetinic acid potently inhibits Kv1.3 potassium channels and T cell activation in human Jurkat T cells

Author:
Xiao-Xing Fu; Li-Li Du; Ning Zhao; Qian Dong; Yu-Hua Liao; Yi-Mei Du
Source:
Journal of ethnopharmacology 2013 v.148 no.2 pp. 647-654
ISSN:
0378-8741
Subject:
T-lymphocytes; Western blotting; calcium; enzyme-linked immunosorbent assay; gene expression; humans; immunomodulation; inhibitory concentration 50; interleukin-2; licorice; patch-clamp technique; potassium channels; quantitative polymerase chain reaction; secretion; traditional medicine
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: Licorice has been extensively used in traditional medicines for treatment of many diseases, including inflammations and immunological disorders. Recent studies have shown that the anti-inflammatory and immunomodulation activities of licorice have been attributed to its active component, glycyrretinic acid (GA). GA consists of two isoforms, 18α- and 18β-. However, it ...
DOI:
10.1016/j.jep.2013.05.022
PubMed:
23707333

63. 18β‐glycyrrhetinic acid improves high‐intensity exercise performance by promoting glucose‐dependent energy production and inhibiting oxidative stress in mice

Author:
Xuan Ma; Hui Chen; Lixing Cao; Shuang Zhao; Chong Zhao; Shutao Yin; Hongbo Hu
Source:
Phytotherapy research 2021 v.35 no.12 pp. 6932-6943
ISSN:
0951-418X
Subject:
Western blotting; athletic performance; bioactive compounds; energy; energy metabolism; enzyme-linked immunosorbent assay; exercise; exercise test; fluorescent antibody technique; glucose transporters; glycogen; insulin; licorice; liver; muscles; oxidative stress; phytotherapy; reactive oxygen species; research
Abstract:
... It has been shown that 18β‐glycyrrhetinic acid (18β‐GA), the main bioactive compound of licorice, can modulate oxidative stress and metabolic processes in liver and skin. Given the critical role of oxidative stress and energy metabolism in exercise‐induced fatigue, we hypothesized that 18β‐GA could exert an ergogenic action by inhibiting excess reactive oxygen species (ROS) induction and promoting ...
DOI:
10.1002/ptr.7310

64. 194 Extracellular vesicles derived from ampullary oviductal fluid improve developmental competence of bovine oocytes

Author:
A. Asaadi; K. Pavani; N. Azari Dolatabadi; P. Van Damme; A. Van Soom
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 225
ISSN:
1031-3613
Subject:
Western blotting; analysis of variance; apoptosis; blastocyst; cows; electron microscopy; epidermal growth factor; follicular fluid; gel chromatography; messenger RNA; microRNA; nanoparticles; oocytes; oviducts; staining; zygote
Abstract:
... Invivo, oocytes mature in the presence of follicular fluid (FF) and ampullary oviductal fluid (AOF), starting from premature to final maturation stages (3-6h postovulation). Extracellular vesicles (EVs) have been identified as important mediators of gamete/embryo-maternal interactions. They carry regulatory molecules, such as microRNAs and mRNAs. So far, the functionality of EVs derived from FF an ...
DOI:
10.1071/RDv32n2Ab194

65. 1α,25-Dihydroxyvitamin D3 promotes angiogenesis by alleviating AGEs-induced autophagy

Author:
Yi Xiong; Feng Zhou; Yeyu Liu; Zumu Yi; Xinyu Wang; Yingying Wu; Ping Gong
Source:
Archives of biochemistry and biophysics 2021 v.712 pp. 109041
ISSN:
0003-9861
Subject:
Western blotting; angiogenesis; autophagy; biophysics; diabetes mellitus; gene expression; inflammation; oxidative stress; vascular endothelial growth factor receptor-1; vascular endothelial growth factor receptor-2
Abstract:
... Diabetes mellitus (DM) induces abnormal angiogenesis and results in multiple chronic vascular complications. Previous studies showed that advanced glycation end products (AGEs) up-regulated in diabetic patients and induced a series of cellular effects such as oxidative stress, inflammation, and autophagy. 1α,25-Dihydroxyvitamin D3 (1,25D), a hormonal form of vitamin D, proved to be beneficial for ...
DOI:
10.1016/j.abb.2021.109041

66. 2,3,5,4′-Tetrahydroxy-stilbene-2-O-beta-d-glucoside induces autophagy-mediated apoptosis in hepatocytes by upregulating miR-122 and inhibiting the PI3K/Akt/mTOR pathway: implications for its hepatotoxicity

Author:
Lei Yang; Wei Xing; Wang-Zhong Xiao; Lin Tang; Lu Wang; Meng-Jiao Liu; Bing Dai
Source:
Pharmaceutical biology 2020 v.58 no.1 pp. 806-814
ISSN:
1744-5116
Subject:
Western blotting; apoptosis; autophagy; bioinformatics; cell viability; flow cytometry; fluorescent antibody technique; genes; hepatocytes; hepatotoxicity; inhibitory concentration 50; luciferase; protein synthesis; quantitative polymerase chain reaction
Abstract:
... The potential hepatotoxicity of Polygoni Multiflori Radix (PMR) has attracted much attention, but the specific mechanism of inducing hepatotoxicity is still unclear due to the complexity of its components. This study investigated the specific mechanism by which 2,3,5,4′-tetrahydroxy-stilbene-2-O-β-d-glucoside (TSG) regulates hepatotoxicity. The toxic effects of TSG (10, 100, 1000 μg/mL) on WRL-68 ...
DOI:
10.1080/13880209.2020.1803367

67. 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside induces autophagy of liver by activating PI3K/Akt and Erk pathway in prediabetic rats

Author:
Xuanbin Wang; Jing Zeng; Xiao Wang; Ju Li; Jin Chen; Ning Wang; Miao Zhang; Yibin Feng; Huailan Guo
Source:
BMC complementary medicine and therapies 2020 v.20 no.1 pp. 177
ISSN:
2662-7671
Subject:
Reynoutria multiflora; Western blotting; active ingredients; alternative medicine; autophagy; blood; blood glucose; blood lipids; body weight; droplets; hepatocytes; herbal medicines; high carbohydrate diet; insulin; insulin resistance; lipids; liver; risk
Abstract:
... BACKGROUND: 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is an active compound derived from Polygonum multiflorum Thunb., a Chinese Taoist herbal medicine, which exerts lipid lowering, anti-cancer, anti-aging, anti-inflammatory and hepatoprotective effects. However, its role in protecting hepatocytes under pre-diabetic condition remains unclear. METHODS: In this study, we developed predia ...
DOI:
10.1186/s12906-020-02949-w

68. 2,3-cis-Procyanidins Elicit Endothelium-Dependent Relaxation in Porcine Coronary Arteries via Activation of the PI3/Akt Kinase Signaling Pathway

Author:
Kaufeld Aurica M.; Pertz Heinz H.; Kolodziej Herbert
Source:
Journal of agricultural and food chemistry 2013 v.61 no.40 pp. 9609-9616
ISSN:
1520-5118
Subject:
NADP (coenzyme); Western blotting; cardiovascular diseases; chemical composition; coronary vessels; foods; fruits; mechanism of action; nitric oxide; oxygen; phosphorylation; proanthocyanidins; procyanidins; risk; signal transduction; swine; xanthine oxidase
Abstract:
... Polyphenols including procyanidins have been reported to reduce the risk of cardiovascular diseases. However, polyphenolic extracts represent complex mixtures, and detailed information on their chemical composition is commonly lacking. The aim of this study was to investigate the potential of a highly purified and chemically defined 2,3-cis-procyanidin sample (di- to hexameric [4β,8]-linked oligom ...
DOI:
10.1021/jf402460m
PubMed:
24032351

69. 2,5-Hexanedione altered the degradation of low-molecular-weight neurofilament in rat nerve tissues

Author:
Fuyong Song; Qingguo Zhang; Ruirui Kou; Chaoshuang Zou; Yuan Gao; Keqin Xie
Source:
Food and chemical toxicology 2012 v.50 no.12 pp. 4277-4284
ISSN:
0278-6915
Subject:
Western blotting; adults; autophagy; hexane; nerve tissue; occupational exposure; pathogenesis; peripheral nervous system diseases; rats; toxicology
Abstract:
... Occupational exposure to n-hexane produces a central–peripheral distal axonopathy, which is characterized by giant axonal swellings filled with neurofilaments (NFs). To investigate the change of NFs degradation and their possible role in n-hexane neuropathy, adult male Wistar rats were administered intraperitoneally at a dosage of 400mg/kg/day 2,5-hexanedione (2,5-HD) for 4weeks. The time course o ...
DOI:
10.1016/j.fct.2012.08.049

70. 2-D PAGE-based comparison of proteasome inhibitor bortezomib in sensitive and resistant mantle cell lymphoma

Author:
Weinkauf, Marc; Zimmermann, Yvonne; Hartmann, Elena; Rosenwald, Andreas; Rieken, Malte; Pastore, Alessandro; Hutter, Grit; Hiddemann, Wolfgang; Dreyling, Martin
Source:
Electrophoresis 2009 v.30 no.6 pp. 974-986
ISSN:
0173-0835
Subject:
RNA; Western blotting; cell division; energy metabolism; gels; gene expression; heat; lymphoma; polyacrylamide gel electrophoresis; proteasome endopeptidase complex; proteins; transcription (genetics); tumor suppressor genes
Abstract:
... Although gene expression following bortezomib treatment has been previously explored, direct effects of bortezomib-induced proteasome inhibition on protein level has not been analyzed so far. Using 2-D PAGE in five mantle cell lymphoma cell lines, we screened for cellular protein level alterations following treatment with 25 nM bortezomib for up to 4 h. Using MS, we identified 38 of the 41 most pr ...
DOI:
10.1002/elps.200800508

71. 2-Hydroxy-3-methylanthraquinone inhibits lung carcinoma cells through modulation of IL-6-induced JAK2/STAT3 pathway

Author:
Chao Sun; Jing Yang; Hai-Bo Cheng; Wei-Xing Shen; Ze-Qun Jiang; Ming-Jie Wu; Li Li; Wen-Ting Li; Ting-Ting Chen; Xi-Wu Rao; Jin-Rong Zhou; Mian-Hua Wu
Source:
Phytomedicine 2019 v.61 pp. 152848
ISSN:
0944-7113
Subject:
Oldenlandia diffusa; Oriental traditional medicine; Western blotting; anthraquinones; antibodies; apoptosis; chemokine CCL5; flow cytometry; gene expression; gene expression regulation; genes; genetic markers; granulocyte colony-stimulating factor; interleukin-6; interleukin-8; lung neoplasms; neoplasm cells; non-specific protein-tyrosine kinase; phosphorylation; protein content; quantitative polymerase chain reaction; tyrosine
Abstract:
... 2-hydroxy-3-methylanthraquinone (HMA), an anthraquinone monomer in traditional Chinese medicine Hedyotis diffusa, has been reported to inhibit the growth of several types of cancer, but its effect on lung cancer has not been adequately investigated.This study aimed to test the hypothesis that HMA inhibit the growth, migration, and invasion of lung cancer cells in part via downregulation of interle ...
DOI:
10.1016/j.phymed.2019.152848

72. 2-Methoxy-1,4-naphthoquinone (MNQ) induces apoptosis of A549 lung adenocarcinoma cells via oxidation-triggered JNK and p38 MAPK signaling pathways

Author:
Jeremy Yee Hoong Ong; Phelim Voon Chen Yong; Yang Mooi Lim; Anthony Siong Hock Ho
Source:
Life sciences 2015 v.135 pp. 158-164
ISSN:
0024-3205
Subject:
DNA damage; Western blotting; adenocarcinoma; apoptosis; cell cycle checkpoints; cell lines; comet assay; cytotoxicity; drug therapy; drugs; flow cytometry; growth retardation; lung neoplasms; lungs; mechanism of action; mitogen-activated protein kinase; neoplasm cells; oxidative stress; reactive oxygen species; signal transduction
Abstract:
... The compound 2-methoxy-1,4-naphthoquinone (MNQ) was previously shown to be cytotoxic against several cancer cell lines, but its mode of action is poorly understood. In this study, we aimed to explore the molecular mechanism of MNQ-induced cytotoxicity of A549 lung adenocarcinoma cells.The growth inhibition potential of MNQ was analyzed using sulforhodamine B assay, flow cytometry cell cycle analys ...
DOI:
10.1016/j.lfs.2015.03.019

73. 2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis

Author:
Mohammed W. Al-Rabia; Mohamed A. Alfaleh; Hani Z. Asfour; Waleed S. Alharbi; Mohamed A. El-Moselhy; Nabil A. Alhakamy; Usama A. Fahmy; Osama A. A. Ahmed; Omar Fahmy; Omar M. Rashad; Abdulmohsin J. Alamoudi; Ashraf B. Abdel-Naim
Source:
Antioxidants 2022 v.11 no.8 pp. -
ISSN:
2076-3921
Subject:
Western blotting; antioxidants; bioavailability; cyclosporine; kidneys; males; micelles; nanocarriers; nephrotoxicity; oral administration; particle size; polyethylene glycol; protein synthesis; succinic acid
Abstract:
... The immunosuppressant cyclosporine A (CSA) has been linked to serious renal toxic effects. Although 2-methoxyestradiol (2ME) possesses a wide range of pharmacological abilities, it suffers poor bioavailability after oral administration. The purpose of this study was to evaluate the potential of 2ME loaded D-ɑ-tocopheryl polyethylene glycol succinate (TPGS) micelles to prevent CSA-induced nephrotox ...
DOI:
10.3390/antiox11081499

74. 2-Methoxyjuglone Induces Apoptosis in HepG2 Human Hepatocellular Carcinoma Cells and Exhibits in Vivo Antitumor Activity in a H22 Mouse Hepatocellular Carcinoma Model

Author:
Yu Heng-Yi; Zhang Xiao-Qiong; Li Xue-; Zeng Fan-Bo; Ruan Han-Li
Source:
Journal of natural products 2013 v.76 no.5 pp. 889-895
ISSN:
1520-6025
Subject:
Juglans; Western blotting; antineoplastic activity; antineoplastic agents; apoptosis; bark; caspase-3; caspase-9; cell cycle checkpoints; cytotoxicity; dose response; flow cytometry; hepatoma; human cell lines; humans; in vivo studies; interphase; mice; mitochondria; models; neoplasm cells; propidium; proteins; shrinkage; staining
Abstract:
... In order to discover anticancer agents from natural sources, an ethanol-soluble extract of the root bark of Juglans cathayensis was investigated and showed cytotoxic effects against various human cancer cell lines. A subsequent phytochemical study on the EtOAc-soluble fraction determined 2-methoxyjuglone (1) as one of the main active constituents. Compound 1 was shown to be cytotoxic against HepG2 ...
DOI:
10.1021/np400025b

75. 2-Methyl-pyran-4-one-3-O-β-d-glucopyranoside isolated from leaves of Punica granatum inhibits the TNFα-induced cell adhesion molecules expression by blocking nuclear transcription factor-κB (NF-κB)

Author:
Sakshi Balwani; Debkumar Nandi; Parasuraman Jaisankar; Balaram Ghosh
Source:
Biochimie 2011 v.93 no.5 pp. 921-930
ISSN:
0300-9084
Subject:
Punica granatum; Western blotting; anti-inflammatory agents; endothelial cells; gel electrophoresis; genes; humans; leaves; mechanism of action; neutrophils; spectral analysis; transcription factor NF-kappa B
Abstract:
... Here, we report bioactivity-guided isolation, purification and characterization of a novel compound, 2-methyl-pyran-4-one-3-O-β-d-glucopyranoside (MPG) from the leaves of Punica granatum. The structure of MPG was established on the basis of its detailed spectral analyses. We demonstrated that MPG not only inhibited the expression of cell adhesion molecules but also significantly blocked its functi ...
DOI:
10.1016/j.biochi.2011.01.010

76. 2-Substituted thiazole-4-carboxamide derivatives as tiazofurin mimics: synthesis and in vitro antitumour activity

Author:
Mirjana Popsavin; Vesna Kojić; Saša Spaić; Miloš Svirčev; Gordana Bogdanović; Dimitar Jakimov; Lidija Aleksić; Velimir Popsavin
Source:
Tetrahedron 2014 v.70 pp. 2343-2350
ISSN:
0040-4020
Subject:
Western blotting; antineoplastic activity; apoptosis; arabinose; caspase-3; cell cycle; chemical reactions; chemical structure; cytotoxicity; flow cytometry; humans; neoplasm cells; organic compounds; xylose
Abstract:
... Tiazofurin analogues bearing a 5-hydroxymethyl-2-methyl-tetrahydrofuro[2,3-d][1,3]dioxol-6-ol moiety as a sugar mimic (2 and 3), and two novel thiazole-based acyclo-C-nucleosides 4 and 16 have been synthesized in multistep sequences starting from d-xylose (compounds 2 and 3) or from d-arabinose (compounds 4 and 16). All synthesized analogues showed potent in vitro antitumour activities against a p ...
DOI:
10.1016/j.tet.2014.02.035

77. 20 (S)-ginsenoside Rh2 inhibits colorectal cancer cell growth by suppressing the Axl signaling pathway in vitro and in vivo

Author:
Haibo Zhang; Jun-Koo Yi; Hai Huang; Sijun Park; Wookbong Kwon; Eungyung Kim; Soyoung Jang; Si-Yong Kim; Seong-kyoon Choi; Duhak Yoon; Sung-Hyun Kim; Kangdong Liu; Zigang Dong; Zae Young Ryoo; Myoung Ok Kim
Source:
Journal of Ginseng Research 2022 v.46 no.3 pp. 396-407
ISSN:
1226-8453
Subject:
Panax; Western blotting; antineoplastic activity; apoptosis; cell cycle checkpoints; cell growth; cell proliferation; colorectal neoplasms; morbidity; mortality; neoplasm cells; research; toxicity; transfection; xenotransplantation
Abstract:
... Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenoside Rh2 (G-Rh2) is a natural compound extracted from ginseng, which exhibits anticancer effects in many cancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo. Cell proliferation, migration, invasion, apoptosis, cell cycle, and western ...
DOI:
10.1016/j.jgr.2021.07.004

78. 20(S)-protopanaxadiol promotes the migration, proliferation, and differentiation of neural stem cells by targeting GSK-3β in the Wnt/GSK-3β/β-catenin pathway

Author:
Kaili Lin; Bin Liu; Sze-Lam Lim; Xiuqiong Fu; Stephen C.-W. Sze; Ken K.-L. Yung; Shiqing Zhang
Source:
Journal of Ginseng Research 2020 v.44 no.3 pp. 475-482
ISSN:
1226-8453
Subject:
Panax; Western blotting; dose response; fluorescent antibody technique; genes; glycogen synthase kinases; mechanism of action; molecular dynamics; mutants; neural stem cells; neurodegenerative diseases; neurons; phosphorylation; protein synthesis; research; transfection
Abstract:
... Active natural ingredients, especially small molecules, have recently received wide attention as modifiers used to treat neurodegenerative disease by promoting neurogenic regeneration of neural stem cell (NSC) in situ. 20(S)-protopanaxadiol (PPD), one of the bioactive ingredients in ginseng, possesses neuroprotective properties. However, the effect of PPD on NSC proliferation and differentiation a ...
DOI:
10.1016/j.jgr.2019.03.001

79. 200 Developing exosomes as a mediator for CRISPR/Cas-9 delivery

Author:
N. Gupta; K. Polkoff; L. Qiao; K. Cheng; J. Piedrahita
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 225
ISSN:
1031-3613
Subject:
DNA; Dependoparvovirus; RNA; Western blotting; computer software; dyes; exosomes; extrusion; fibroblasts; filtration; flow cytometry; gene editing; green fluorescent protein; ice; immune response; immune system; lipids; lipophilicity; molecular weight; particle size; peptidase K; polymerase chain reaction; saponins; sonication; staining; swine; therapeutics; transgenes; transmission electron microscopy
Abstract:
... CRISPR/Cas systems present a powerful gene-editing tool with the potential for widespread therapeutic use; however, current methods of in vivo delivery such as adeno-associated viruses (AAV) may stimulate an immune response, creating the need for an alternative for delivery of CRISPR/Cas9. Exosomes are small vesicles that are released by cells and serve as a delivery system for RNA, proteins, and ...
DOI:
10.1071/RDv31n1Ab200

80. 205 Towards the correction of meconium ileus with cystic fibrosis transmembrane conductance regulator (CFTR) intestinal expression in CFTR knockout sheep

Author:
I. Viotti Perisse; Z. Fan; Y. Liu; M. Regouski; A. Van Wettere; Z. Wang; A. Harris; K. L. White; I. A. Polejaeva
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 228
ISSN:
1031-3613
Subject:
Western blotting; complementary DNA; cystic fibrosis; cystic fibrosis transmembrane conductance regulator; death; embryo transfer; epithelial cells; fatty acid-binding proteins; females; fibroblasts; fibrosis; gall bladder; gene expression; genetic vectors; hyperplasia; intestinal obstruction; intestines; lambs; males; mutation; necropsy; neonates; polymerase chain reaction; pregnancy; progeny; rats; sequence analysis; somatic cells; transfection; transgenes; transgenic animals
Abstract:
... Cystic fibrosis (CF) is a human autosomal genetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is responsible for Cl − anion transport in epithelial cells. We previously generated CFTR +/− and CFTR −/− lambs using CRISPR/Cas9 and somatic cell nuclear transfer (SCNT) techniques. The CFTR −/− lambs display many features similar to humans w ...
DOI:
10.1071/RDv31n1Ab205

81. 207 ganglioside improves the meiotic maturation and anti-apoptotic effects during in vitro maturation of porcine oocytes

Author:
J.-W. Kim; H.-J. Park; S.-K. Chae; J.-H. Ahn; G.-Y. Do; J.-Y. Park; S.-G. Yang; D.-B. Koo
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 234-235
ISSN:
1031-3613
Subject:
Western blotting; apoptosis; blastocyst; cell differentiation; embryogenesis; epidermal growth factor; epidermal growth factor receptors; gangliosides; germinal vesicle; hydrogen peroxide; metaphase; mice; pronucleus; signal transduction; swine; tyrosine
Abstract:
... Gangliosides are sialic-acid-glycosphingolipids expressed in almost all vertebrate cells. These play a role in regulating cell differentiation and signal molecules of cellular signaling, including interactions with molecules of signal transduction pathways, such as receptors tyrosine kinases (RTK). Gangliosides perform critical functions through interactions with proteins as receptors in cell-cell ...
DOI:
10.1071/RDv28n2Ab206

82. 20‐Hydroxyecdysone‐upregulated proteases involved in Bombyx larval fat body destruction

Author:
S.‐Y. Guo; W.‐M. Wu; S.‐Y. Li; Y. Liu; Z. F. Ruan; M.‐Q. Ye; Y. Xiao; Y.‐J. Zhong; Y. Cao; K. Li; L. Tian
Source:
Insect molecular biology 2018 v.27 no.6 pp. 724-738
ISSN:
0962-1075
Subject:
Bombyx; RNA interference; Western blotting; cathepsin B; cathepsin D; cathepsin L; cysteine proteinase inhibitors; ecdysterone; fat body; gene expression; insects; larvae; liver; mammals; messenger RNA; metalloproteinases; metamorphosis; molting; prepupae; programmed cell death; protein content; staining
Abstract:
... During insect larval–pupal metamorphosis, the obsolete larval organs and tissues undergo histolysis and programmed cell death to recycle cellular materials. It has been demonstrated that some cathepsins are essential for histolysis in larval tissues, but the process of tissue destruction is not well documented. Fat body, the homologous organ to mammalian liver and adipose tissue, goes through a di ...
DOI:
10.1111/imb.12511

83. 22(R)-hydroxycholesterol and pioglitazone synergistically decrease cholesterol ester via the PPARγ–LXRα–ABCA1 pathway in cholesterosis of the gallbladder

Author:
Jing-Min Wang; Dong Wang; Yu-Yan Tan; Gang Zhao; Zhen-Ling Ji
Source:
Biochemical and biophysical research communications 2014 v.447 pp. 152-157
ISSN:
0006-291X
Subject:
ABC transporters; Western blotting; biosynthesis; cholesterol; cholesterol metabolism; cholesteryl esters; cytoplasm; droplets; epithelial cells; gall bladder; homeostasis; liver; peroxisome proliferator-activated receptors; small interfering RNA; staining; steroid hormones
Abstract:
... Cholesterosis is a disease of cholesterol metabolism characterized by the presence of excessive lipid droplets in the cytoplasm. These lipid droplets are mainly composed of cholesterol esters derived from free cholesterol. The removal of excess cholesterol from gallbladder epithelial cells (GBECs) is very important for the maintenance of intracellular cholesterol homeostasis and the preservation o ...
DOI:
10.1016/j.bbrc.2014.03.130

84. 22-O-(N-Boc-L-glycine) ester of renieramycin M inhibits migratory activity and suppresses epithelial–mesenchymal transition in human lung cancer cells

Author:
Yamin Oo; Justin Quiel Lasam Nealiga; Khanit Suwanborirux; Supakarn Chamni; Gea Abigail Uy Ecoy; Varisa Pongrakhananon; Pithi Chanvorachote; Chatchai Chaotham
Source:
Natural medicines 2021 v.75 no.4 pp. 949-966
ISSN:
1340-3443
Subject:
Western blotting; actin; alkaloids; cadherins; dose response; humans; lung neoplasms; metalloproteinases; metastasis; migratory behavior; mortality
Abstract:
... The incidence of metastasis stage crucially contributes to high recurrence and mortality rate in lung cancer patients. Unfortunately, no available treatment inhibits migration, a key metastasis process in lung cancer. In this study, the effect of 22-O-(N-Boc-L-glycine) ester of renieramycin M (22-Boc-Gly-RM), a semi-synthetic amino ester derivative of bistetrahydroisoquinolinequinone alkaloid isol ...
DOI:
10.1007/s11418-021-01549-3

85. 23-O-acetylshengmanol-3-O-α-L-arabinoside alleviates lipopolysaccharide-induced acute lung injury through inhibiting IκB/NF-κB and MAPK/AP-1 signaling pathways

Author:
Chunyan Chen; Lin Li; Xiaomei Liu; Deqin Zhang; Yang Liu; Yuhong Li
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115725
ISSN:
0378-8741
Subject:
Actaea cimicifuga; Oriental traditional medicine; Western blotting; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; fluorescent antibody technique; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; phosphorylation; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
DOI:
10.1016/j.jep.2022.115725

86. 237 effect of a pten inhibitor on ovarian contents of apoptosis-related proteins and anti-müllerian hormone (amh) in a/j mice

Author:
O. Suzuki
Source:
Reproduction, fertility, and development 2016 v.28 no.2 pp. 250
ISSN:
1031-3613
Subject:
Western blotting; actin; analysis of variance; animal ovaries; caspase-3; caspase-9; chromosomes; enzyme-linked immunosorbent assay; females; follicular atresia; mice; ovulation; pregnant mare serum gonadotropin; radioimmunoassays; signal transduction; superovulation; Japan
Abstract:
... Strain/individual differences in superovulation efficiency with gonadotropins constitute a serious problem in mouse reproduction (Suzuki et al. 1996 Reprod. Fertil. Dev. 8, 975–980). Because PI3K/Akt signalling pathway is involved in ovarian folliculogenesis and phosphatase and tensin homologue deleted from chromosome 10 (PTEN) negatively regulates the process, I have been studying the effect of P ...
DOI:
10.1071/RDv28n2Ab236

87. 25-O-acetyl-23,24-dihydro-cucurbitacin F induces cell cycle G2/M arrest and apoptosis in human soft tissue sarcoma cells

Author:
Birgit Lohberger; Nadine Kretschmer; Eva Bernhart; Beate Rinner; Nicole Stuendl; Heike Kaltenegger; Stefan Kahl; Rudolf Bauer; Andreas Leithner
Source:
Journal of ethnopharmacology 2015 v.164 pp. 265-272
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Quisqualis; Western blotting; apoptosis; branches; caspase-3; cell cycle checkpoints; cell proliferation; cell viability; cyclin-dependent kinase; cyclins; cytotoxicity; dose response; drug therapy; human cell lines; leaves; metastasis; mitosis; neoplasm cells; pro-apoptotic proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sarcoma; tissues
Abstract:
... Quisqualis indica is used in traditional Chinese medicine to treat cancer and related syndromes and also known for its anthelminthic effects.Soft tissue sarcomas represent a rare group of malignant tumors that frequently exhibit chemotherapeutic resistance and increased metastatic potential. In this study, we evaluated the cytotoxic, apoptosis inducing and cell cycle arresting effects of 25-O-acet ...
DOI:
10.1016/j.jep.2015.02.023

88. IL- 27 along with IL-28B ameliorates the pulmonary redox impairment, inflammation and immunosuppression in benzo(a)pyrene induced lung cancer bearing mice

Author:
Debabrata Majumder; Rahul Debnath; Debasish Maiti
Source:
Life sciences 2020 v.260 pp. 118384
ISSN:
0024-3205
Subject:
Western blotting; albino; angiogenesis; carcinogenesis; cell proliferation; immunohistochemistry; immunosuppression; immunotherapy; inflammation; lung neoplasms; lungs; quantitative polymerase chain reaction; smoke; tobacco; toxicity testing; vehicles (equipment)
Abstract:
... The major cause behind lung cancer development is exposure to various polycyclic aromatic hydrocarbons like benzo(a)pyrene (BaP) present in tobacco smoke, motor vehicle, and industrial exhaust. BaP is reported to induce the expression of various pro-inflammatory cytokines and matrix remodeling proteins. It is also responsible for dysfunction and exhaustion of the killing capacity of CD8+ T lymphoc ...
DOI:
10.1016/j.lfs.2020.118384

89. IL- 27 attenuates the TGF-β1-induced proliferation, differentiation and collagen synthesis in lung fibroblasts

Author:
Zhaoxing Dong; Xinxiang Zhao; Wenlin Tai; Wen Lei; Yin Wang; ZhenKun Li; Tao Zhang
Source:
Life sciences 2016 v.146 pp. 24-33
ISSN:
0024-3205
Subject:
Western blotting; cell cycle; collagen; fibroblasts; fibrosis; fluorescent antibody technique; gene therapy; lungs; mortality; quantitative polymerase chain reaction; respiratory tract diseases; signal transduction; staining; transforming growth factor beta 1
Abstract:
... Pulmonary fibrosis is a type of chronic lung disease and has characteristics that progress quickly, has a high fatality rate and a poor therapeutic effect. Our previous research showed that interleukin-27(IL-27) potentially attenuates BLM-induced pulmonary fibrosis, but the function of IL-27 in lung fibroblasts (LFs) differentiation pulmonary fibrosis is yet to be known.Here we investigated the ef ...
DOI:
10.1016/j.lfs.2016.01.004

90. IL- 27 suppresses SKOV3 cells proliferation by enhancing STAT3 and inhibiting the Akt signal pathway

Author:
Zhu Zhang; Bin Zhou; Kui Zhang; Yaping Song; Lin Zhang; Mingrong Xi
Source:
Molecular Immunology 2016 v.78 pp. 155-163
ISSN:
0161-5890
Subject:
Toll-like receptor 4; Western blotting; animal ovaries; antineoplastic activity; apoptosis; caspase-3; cell proliferation; cell viability; cytotoxicity; epithelium; genes; interferon-gamma; interleukin-12; interleukin-6; lactate dehydrogenase; messenger RNA; neoplasm cells; ovarian neoplasms; plasmids; signal transduction
Abstract:
... Ovarian cancer continues to be the most lethal gynecologic malignancy worldwide. IL-27 is a novel member of the IL-12 cytokine family. The aim of this study was to investigate the effects of IL-27 on the ovarian cystadenocarcinoma cell line SKOV3 and determine possible mechanisms underlying its effect. We stably transfected an IL-27 plasmid, empty vector, IL-27 shRNA or negative control into SKOV3 ...
DOI:
10.1016/j.molimm.2016.09.014

91. A 28.6-kD small heat shock protein (MnHSP28.6) protects Macrobrachium nipponense against heavy metal toxicity and oxidative stress by virtue of its anti-aggregation activity

Author:
Fengyu Yuan; Zilan Yang; Ting Tang; Song Xie; Fengsong Liu
Source:
Fish & shellfish immunology 2019 v.95 pp. 635-643
ISSN:
1050-4648
Subject:
Escherichia coli; Macrobrachium nipponense; Western blotting; bacterial infections; cadmium; citrate (si)-synthase; copper; doxorubicin; genes; heat shock proteins; heavy metals; homeostasis; hydrogen peroxide; insulin; muscles; oxidative stress; protective effect; protein denaturation; quantitative polymerase chain reaction; superoxide dismutase; tissue distribution; toxicity
Abstract:
... Small heat shock proteins (sHSPs) are ATP-independent chaperones and involved into various physiological and stress processes. In the present study, a 28.6-kD sHSP coding gene, MnHSP28.6, was cloned and characterized from the oriental river prawn Macrobrachium nipponense. Tissue distribution analysis via qPCR and western blot revealed that MnHSP28.6 predominantly expressed in muscle. The temporal ...
DOI:
10.1016/j.fsi.2019.10.053

92. A 29-kDa merozoite protein is a prospective antigen for serological diagnosis of Babesia orientalis infection in buffaloes

Author:
Zhou, D.N.; Du, F.; Liu, Q.; Zhou, Y.Q.; Zhao, J.L.
Source:
Veterinary parasitology 2009 v.162 no.1-2 pp. 1-6
ISSN:
0304-4017
Subject:
buffaloes; Babesia; animal pathogens; babesiosis; antigens; animal proteins; serodiagnosis; complementary DNA; open reading frames; monoclonal antibodies; Western blotting; recombinant proteins; polymerase chain reaction
Abstract:
... A gene encoding a 29-kDa protein of Babesia orientalis (BoP29) was isolated by immunoscreening the B. orientalis cDNA expression library using monoclonal antibodies and its nucleotide sequence of the cDNA was 971bp with an open reading frame of 750bp. The results of BLASTn and BLASTx in NCBI showed that this cDNA sequence had similarity with Babesia bovis hypothetical protein (BBOV_II005480). West ...
DOI:
10.1016/j.vetpar.2009.03.001
PubMed:
19345505

93. 2α-Hydroxyursolic Acid Inhibited Cell Proliferation and Induced Apoptosis in MDA-MB-231 Human Breast Cancer Cells through the p38/MAPK Signal Transduction Pathway

Author:
Xue Jiang; Tong Li; Rui Hai Liu
Source:
Journal of agricultural and food chemistry 2016 v.64 no.8 pp. 1806-1816
ISSN:
0021-8561
Subject:
Western blotting; antineoplastic activity; apoptosis; breast neoplasms; caspase-3; cell proliferation; cyclins; cytotoxicity; humans; mechanism of action; methylene blue; mitogen-activated protein kinase; neoplasm cells; proliferating cell nuclear antigen; signal transduction
Abstract:
... The mechanisms of action of 2α-hydroxyursolic acid in inhibiting cell proliferation and inducing apoptosis in MDA-MB-231 human breast cancer cells were investigated. The antiproliferative activity and cytotoxicity were determined by the methylene blue assay. The expression of proteins was determined using Western blot. 2α-Hydroxyursolic acid significantly inhibited MDA-MB-231 cell proliferation, a ...
DOI:
10.1021/acs.jafc.5b04852

94. 2′, 5′-Oligoadenylate Synthetase 2 (OAS2) Inhibits Zika Virus Replication through Activation of Type Ι IFN Signaling Pathway

Author:
Xinzhong Liao; He Xie; Shilin Li; Haiyan Ye; Shuang Li; Kai Ren; Yujia Li; Min Xu; Wenyu Lin; Xiaoqiong Duan; Chunhui Yang; Limin Chen
Source:
Viruses 2020 v.12 no.4 pp. -
ISSN:
1999-4915
Subject:
Western blotting; Zika virus; gene overexpression; genes; interferons; luciferase; messenger RNA; plasmids; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sequence analysis; signal transduction; small interfering RNA; transfection; virus replication
Abstract:
... Background: 2′, 5′-oligoadenylate synthetase 2 (OAS2) has been known as an antiviral interferon-stimulated gene (ISG). However, the role of OAS2 on Zika virus (ZIKV) replication is still unknown. In this study, we sought to explore the effect of OAS2 on ZIKV replication and its underlying mechanism. Methods: We performed RNA-Seq in A549 cells with or without ZIKV infection. OAS2 or RIG-I was overe ...
DOI:
10.3390/v12040418

95. 3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

Author:
Jiajun Liu; Wen Hu; Xiaoying Ma; Xin Liang; Long Lin; Jianming Huang; Jianwen Liu
Source:
Environmental toxicology 2022 v.37 no.2 pp. 349-361
ISSN:
1520-4081
Subject:
Azolla; Western blotting; agonists; apoptosis; caspases; cell lines; cytotoxicity; digestive system; ecotoxicology; epithelial cells; immune system; membrane potential; mitochondria; radioprotective effect; radiotherapy
Abstract:
... Ionizing radiation (IR) brings many health problems to humans, causing damage to the digestive system, hematopoietic system, and immune system. Natural compounds derived from plants have attracted widespread attention due to their low toxicity. Here, we found that 3,4,5‐O‐tricaffeoylquinic acid (tCQA) extracted from natural plant Azolla imbricata could significantly alleviate the systemic damage i ...
DOI:
10.1002/tox.23403

96. 3,4-Dihydroxyphenylacetic acid ameliorates gut barrier dysfunction via regulation of MAPK-MLCK pathway in type 2 diabetes mice

Author:
Min Liu; Li Wang; Bijun Huang; Qun Lu; Rui Liu
Source:
Life sciences 2022 v.305 pp. 120742
ISSN:
0024-3205
Subject:
Western blotting; absorption barrier; anti-inflammatory activity; blood glucose; body weight; digestive system diseases; glutathione; high fat diet; hyperglycemia; inflammation; insulin; insulin resistance; interleukin-10; intestines; lipopolysaccharides; malondialdehyde; mitogen-activated protein kinase; myosin; noninsulin-dependent diabetes mellitus; oxidative stress; protein synthesis; streptozotocin; superoxide dismutase; tight junctions
Abstract:
... This study aims to investigate whether 3,4-dihydroxyphenylacetic acid (DHAA) can improve gut barrier function by inhibiting the mitogen-activated protein kinase (MAPK) - myosin light-chain kinase (MLCK) signaling pathway in type 2 diabetes (T2D) mice. T2D mice were induced by a high-fat diet combined with streptozotocin. T2D mice were intragastrically administered with DHAA at 75 and 150 mg kg/bod ...
DOI:
10.1016/j.lfs.2022.120742

97. 3,4-Dihydroxyphenylethanol ameliorates lipopolysaccharide-induced septic cardiac injury in a murine model

Author:
Lu Zhang; Kun Wen; Zhiqiang Zhang; Chengen Ma; Ni Zheng
Source:
Open life sciences 2021 v.16 no.1 pp. 1313-1320
ISSN:
2391-5412
Subject:
Western blotting; animal models; antioxidants; apoptosis; blood serum; caspase-3; interleukin-6; olive oil; oxidative stress; polyphenols; protective effect; protein synthesis; troponin T
Abstract:
... 3,4-Dihydroxyphenylethanol (DOPET) is a polyphenol found in olive oil. The present study evaluated the protective role of DOPET on LPS provoked septic cardiac injury in a murine model. Four groups were used in the study (n = 3): control, LPS, DOPET alone, and DOPET + LPS. LPS (15 mg/kg; i.p.); they were used to induce cardiac sepsis. The cardiac markers like LDH, CK-MB, and troponin-T, as well as ...
DOI:
10.1515/biol-2021-0125

98. 3,4-Methylenedioxy-β-nitrostyrene inhibits adhesion and migration of human triple-negative breast cancer cells by suppressing β1 integrin function and surface protein disulfide isomerase

Author:
I-Hua Chen; Fang-Rong Chang; Yang-Chang Wu; Po-Hsiung Kung; Chin-Chung Wu
Source:
Biochimie 2015 v.110 pp. 81-92
ISSN:
0300-9084
Subject:
CD29 antigen; Western blotting; actin; adhesion; antineoplastic agents; breast neoplasms; cell adhesion; cell viability; confocal microscopy; extracellular matrix; immigration; metastasis; neoplasm cells; non-specific protein-tyrosine kinase; phosphorylation; protein disulfide-isomerase; surface proteins; thiols
Abstract:
... Triple negative breast cancer (TNBC) exhibits an aggressive clinical course by high metastatic potential. It is known that integrin-mediated cell adhesion and migration are important for cancer metastasis. In the present study, a synthetic compound, 3, 4-methyenedioxy-β-nitrostyrene (MNS), significantly inhibited adhesion of TNBC cell lines to different extracellular matrix (ECM) components. The a ...
DOI:
10.1016/j.biochi.2015.01.006

99. 3-Bromo-7-nitroindazole attenuates brain ischemic injury in diabetic stroke via inhibition of endoplasmic reticulum stress pathway involving CHOP

Author:
Krishnamoorthy Srinivasan; Shyam S. Sharma
Source:
Life sciences 2012 v.90 no.3-4 pp. 154-160
ISSN:
0024-3205
Subject:
DNA fragmentation; Western blotting; apoptosis; binding proteins; brain; edema; endoplasmic reticulum; glucose; high fat diet; immunohistochemistry; infarction; ischemia; models; neuronal nitric oxide synthase; neuroprotective effect; nitric oxide; noninsulin-dependent diabetes mellitus; pathogenesis; rats; streptozotocin; stroke
Abstract:
... Aims The role of nitric oxide (NO) and endoplasmic reticulum (ER) stress has been implicated in the pathogenesis of cerebral ischemic/reperfusion (I/R) injury and diabetes. The aim of the study was to investigate the neuroprotective potential of 3-bromo-7-nitroindazole (3-BNI), a potent and selective neuronal nitric oxide synthase (nNOS) inhibitor against ER stress and focal cerebral I/R injury as ...
DOI:
10.1016/j.lfs.2011.10.017

100. 3-Bromopyruvate decreased kidney fibrosis and fibroblast activation by suppressing aerobic glycolysis in unilateral ureteral obstruction mice model

Author:
Honglin Yu; Jingbo Zhu; Lingyu Chang; Chaozhao Liang; Xiaohu Li; Wei Wang
Source:
Life sciences 2021 v.272 pp. 119206
ISSN:
0024-3205
Subject:
Western blotting; collagen; dose response; fibroblasts; fibrosis; fluorescence microscopy; fluorescent antibody technique; glycolysis; immunohistochemistry; interleukin-1; isozymes; kidneys; lactate dehydrogenase; liver; mice; motivation; pyruvate kinase; quantitative polymerase chain reaction; rats; toxicity testing
Abstract:
... Enhanced aerobic glycolysis is a motivation of fibroblast–myofibroblast transdifferentiation (FMT), leading to kidney fibrosis. 3-Bromopyruvate (3-BrPA) is a glycolysis inhibitor and has fibrosis-protected effect in liver. This study aims to explore the effects of 3-BrPA on aerobic glycolysis and kidney fibrosis in a unilateral ureteral obstruction (UUO) mice model and transforming growth factor-β ...
DOI:
10.1016/j.lfs.2021.119206