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1. 23-O-acetylshengmanol-3-O-α-L-arabinoside alleviates lipopolysaccharide-induced acute lung injury through inhibiting IκB/NF-κB and MAPK/AP-1 signaling pathways

Author:
Chunyan Chen; Lin Li; Xiaomei Liu; Deqin Zhang; Yang Liu; Yuhong Li
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115725
ISSN:
0378-8741
Subject:
Actaea cimicifuga; Oriental traditional medicine; Western blotting; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; fluorescent antibody technique; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; phosphorylation; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
DOI:
10.1016/j.jep.2022.115725

2. Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221

Author:
Jie Yang; Zi-Xuan Wang; Lei Fang; Tu-Shuai Li; Zhi-Hong Liu; Ying Pan; Ling-Dong Kong
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115688
ISSN:
0378-8741
Subject:
Atractylodes lancea; Magnolia officinalis; Oriental traditional medicine; Western blotting; adiponectin receptors; agonists; animal models; diet; fluorescent antibody technique; fructose; humans; insulin; insulin resistance; kidneys; liquid chromatography; non-specific serine/threonine protein kinase; pharmacology; rats; tandem mass spectrometry; therapeutics; thermosensitivity
Abstract:
... In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu's functions including Kidney deficiency. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection. To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by hig ...
DOI:
10.1016/j.jep.2022.115688

3. Calcium pyruvate attenuates fat deposition by augmenting fatty acid oxidation and inhibiting glucose oxidation in juvenile large yellow croaker (Larimichthys crocea) consuming a high-fat diet

Author:
Yunqiang Zhang; Wencong Lai; Qiuchi Chen; Wei Fang; Kun Cui; Yuliang He; Xueshan Li; Kangsen Mai; Qinghui Ai
Source:
Aquaculture 2023 v.562 pp. 738778
ISSN:
0044-8486
Subject:
AMP-activated protein kinase; Larimichthys crocea; Western blotting; acetyl-CoA carboxylase; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; beta oxidation; blood glucose; calcium; carnitine palmitoyltransferase; catalase; citrates; diacylglycerol acyltransferase; dietary supplements; droplet size; fatty acid transport proteins; fatty-acid synthase; fish; gene expression; glucokinase; glucose; growth performance; hepatosomatic index; hexokinase; high density lipoprotein cholesterol; high fat diet; interleukin-1beta; juveniles; lipemic effect; lipogenesis; liver; low density lipoprotein cholesterol; malate dehydrogenase; muscles; obesity; oxidation; phosphofructokinases; prostaglandin synthase; protein synthesis; pyruvate dehydrogenase (lipoamide); pyruvate kinase; pyruvic acid; succinate dehydrogenase (quinone); triacylglycerol lipase; triacylglycerols; weight gain
Abstract:
... Calcium pyruvate (CP), known as an energetic aid, has been shown to enhance lipid utilization and prevent obesity in mammals. However, very few studies were conducted to evaluate the potential implications of CP for aquatic animals. This study examined the role of CP on fat deposition in large yellow croaker by feeding fish with high-fat diets containing four levels of dietary CP (0, 0.375%, 0.75% ...
DOI:
10.1016/j.aquaculture.2022.738778

4. Celastrus orbiculatus Thunb. extract inhibits EMT and metastasis of gastric cancer by regulating actin cytoskeleton remodeling

Author:
Haibo Wang; YuanYuan Luo; Shiya Ou; Tengyang Ni; Zewen Chu; Xinyi Feng; Xiaojun Dai; Xiaochun Zhang; Yanqing Liu
Source:
Journal of ethnopharmacology 2023 v.301 pp. 115737
ISSN:
0378-8741
Subject:
Celastrus orbiculatus; Oriental traditional medicine; Western blotting; actin; analgesics; animals; cell movement; medicinal plants; metalloproteinases; metastasis; microfilaments; neoplasm cells; protein degradation; regulatory proteins; stomach neoplasms; China
Abstract:
... The traditional Chinese medicine herb Celastrus orbiculatus Thunb. is an important folk medicinal plant in China that has been used as an anti-inflammatory, antitumor, and analgesic in various diseases. Recent years, many studies have reported the significant effects of Celastrus orbiculatus Thunb. extract (COE) on gastric cancer. However, the specific mechanism by which COE regulates gastric canc ...
DOI:
10.1016/j.jep.2022.115737

5. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
DNA; DNA damage; Sparus aurata; Western blotting; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

6. Uncovering the effect and mechanism of Panax notoginseng saponins on metabolic syndrome by network pharmacology strategy

Author:
Yisa Wang; Peng Ma; Zijing Wang; Mingxia Sun; Biyu Hou; Tianshu Xu; Wenlan Li; Xiuying Yang; Guanhua Du; Tengfei Ji; Guifen Qiang
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115680
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Panax notoginseng; Western blotting; adipocytes; adiposity; blood glucose; blood stasis; brown adipose tissue; computer simulation; drugs; energy; fatty liver; glucose; heat production; high fat diet; histopathology; insulin tolerance test; liver; metabolic diseases; metabolic syndrome; metabolism; obesity; pharmacology; protective effect; quantitative polymerase chain reaction; saponins; stomach; therapeutics; weight loss; white adipose tissue
Abstract:
... Metabolic syndrome (MetS) is a cluster of disease centered on obesity, which is the result of stagnation of liver qi according to traditional Chinese medicine. Panax notoginseng is a traditional Chinese herbal medicine, entering liver and stomach meridians and dissipating blood stasis, in which panax notoginseng saponins (PNS) are the main active components. However, its effects and mechanism on m ...
DOI:
10.1016/j.jep.2022.115680

7. Xianglian Pill attenuates ulcerative colitis through TLR4/MyD88/NF-κB signaling pathway

Author:
Yuxin Dai; Qiulu Lu; Peiyi Li; Junyu Zhu; Jiaxin Jiang; Tong Zhao; Yue Hu; Kang Ding; Min Zhao
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115690
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Western blotting; blood serum; colon; dextran sulfate; enzyme activity; enzyme-linked immunosorbent assay; fluorescent antibody technique; histopathology; interleukin-18; interleukin-6; liquid chromatography; models; myeloperoxidase; necrosis; neoplasms; protein domains; quantitative polymerase chain reaction; salicylic acid; ubiquitination; ulcerative colitis
Abstract:
... Xianglian Pill (XLP) is a classical Chinese medicine prescription applied for controlling ulcerative colitis (UC). Whereas, the underlying mechanism remains unclear. The present work was aimed to investigate the mechanism of XLP in dextran sulfate sodium (DSS)-induced UC via the Toll Like Receptor 4 (TLR4)/Myeloid Differentiation factor 88 (MyD88)/Nuclear Factor kappa-B (NF-κB) signaling in mice.  ...
DOI:
10.1016/j.jep.2022.115690

8. The crude extract from the flowers of Trollius chinensis Bunge exerts anti-influenza virus effects through modulation of the TLR3 signaling pathway

Author:
Yuxi Liang; Xiaoli Liu; Jingyan Hu; Songli Huang; Xin Ma; Xiaoyan Liu; Rufeng Wang; Xiuhua Hu
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115743
ISSN:
0378-8741
Subject:
Influenza A virus; Oriental traditional medicine; Trollius chinensis; Western blotting; blood; cytoplasm; epithelium; fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
DOI:
10.1016/j.jep.2022.115743

9. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

10. (±)-Equol interferes with the secretion of gonadotropin-releasing hormone and the expression of neuromodulators in murine neurons

Author:
Jingyuan Xiong; Minghui Wang; Ye Tian; Aru Ling; Shufang Shan; Li Zhao; Guo Cheng
Source:
Toxicological and environmental chemistry 2022 v.104 no.1 pp. 129-140
ISSN:
1029-0486
Subject:
RING finger domains; Western blotting; cell viability; daidzein; environmental science; enzyme-linked immunosorbent assay; estrogens; females; gonadotropin-releasing hormone; intestines; menopause; metabolites; mice; neurotransmitters; quantitative polymerase chain reaction; secretion; toxicology
Abstract:
... S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the ef ...
DOI:
10.1080/02772248.2022.2055031

11. (−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma

Author:
I-Ta Lu; Shih-Chao Lin; Yi-Chia Chu; Ya Wen; You-Cheng Lin; Wen-Chien Cheng; Jyh-Horng Sheu; Chi-Chien Lin
Source:
Marine drugs 2022 v.20 no.2 pp. -
ISSN:
1660-3397
Subject:
Western blotting; antibacterial properties; apoptosis; caspases; cell viability; death domain receptors; endoplasmic reticulum; guanidines; hepatoma; humans; liver; membrane potential; methanol; mitochondria; mitochondrial membrane; sesquiterpenoids
Abstract:
... Liver cancers, such as hepatocellular carcinoma (HCC), are a highly prevalent cause of cancer-related deaths. Current treatments to combat liver cancer are limited. (−)-Agelasidine A, a compound isolated from the methanol extract of Agelasnakamurai, a sesquiterpene guanidine derived from sea sponge, has antibacterial activity. We demonstrated its anticancer capabilities by researching the associat ...
DOI:
10.3390/md20020109

12. (−)-Epicatechin modulates the expression of myomiRs implicated in exercise response in mouse skeletal muscle

Author:
Carlos Palma-Flores; Paola B. Zárate-Segura; J. Manuel Hernández-Hernández; Sergio de los Santos; Andrea S. Tejeda-Gómez; Luis Javier Cano-Martínez; Patricia Canto; Jorge Omar Garcia-Rebollar; Ramón M. Coral-Vázquez
Source:
Gene 2023 v.849 pp. 146907
ISSN:
0378-1119
Subject:
Western blotting; bioinformatics; exercise; genes; hypertrophy; mice; microRNA; muscle development; muscles; myogenin; signal transduction; skeletal muscle
Abstract:
... The flavanol (−)-epicatechin has exercise-mimetic properties. Besides, several miRNAs play a role in modulating the adaptation of the muscle to different training protocols. However, notwithstanding all information, few studies aimed to determine if (−)-epicatechin can modify the expression of miRNAs related to skeletal muscle development and regeneration. Mice were treated for fifteen days by ora ...
DOI:
10.1016/j.gene.2022.146907

13. 17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner

Author:
Ana Laura Pereira-Suárez; María de la Luz Galván-Ramírez; Laura Rocío Rodríguez-Pérez; Edgar I. López-Pulido; Christian David Hernández-Silva; Inocencia Guadalupe Ramírez-López; Grecia Viridiana Morales Amaya; Luis David Lopez Cabrera; José Francisco Muñoz-Valle; Adrián Ramírez-de-Arellano
Source:
Molecular and biochemical parasitology 2022 v.247 pp. 111433
ISSN:
0166-6851
Subject:
Toxoplasma gondii; Western blotting; cell growth; estrogen receptors; inflammation; macrophages; models; monocytes; parasites; parasitology; phosphatidylinositol 3-kinase; pregnancy; prolactin receptors; tachyzoites
Abstract:
... Toxoplasma gondii (T. gondii) is a parasite common in pregnancy. Monocytes and macrophages are a significant immunologic barrier against T. gondii by boosting up inflammation. This outcome is highly regulated by signaling pathways such as MAPK (ERK1/2) and PI3K (AKT), necessary in cell growth and proliferation. It may be associated with the hormonal receptors’ modulation by T. gondii (Estrogen Rec ...
DOI:
10.1016/j.molbiopara.2021.111433

14. 17β-estradiol reduces NF-κB expression induced by increased crosstalk between KLF5 and ERα in murine vascular smooth muscle cells

Author:
Man-li Zhang; Man-na Zhang; Wen-li Wang; Hui Chen; Xia Wang; Xuan Li; Li Li; Fei Tong
Source:
Biochemical and biophysical research communications 2022 v.625 pp. 181-187
ISSN:
0006-291X
Subject:
Western blotting; cardiovascular system; cell proliferation; chromatin immunoprecipitation; fluorescent antibody technique; inflammation; luciferase; research; smooth muscle
Abstract:
... Pathological vascular remodeling and cell proliferation play vital roles in many proliferative vascular diseases. Estrogen can protect the cardiovascular system, but its exact molecular mechanism is unknown. Here we report that 17β-estradiol (E2) suppressed vascular smooth muscle cells (VSMCs) proliferation and inflammation. qRT-PCR and Western blot demonstrated that E2 decreased NF-κB p50 express ...
DOI:
10.1016/j.bbrc.2022.07.054

15. 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model

Author:
Mojtaba Malekinejad; Mohammad Reza Pashaee; Hassan Malekinejad
Source:
European journal of nutrition 2022 v.61 no.7 pp. 3437-3447
ISSN:
1436-6207
Subject:
Western blotting; absorption; adhesion; biochemical pathways; cell viability; cytotoxicity; dietary supplements; electrical resistance; epithelium; glycyrrhizin; human cell lines; humans; intestines; luciferase; metabolites; models; occludins; permeability; protein content; tight junctions; toxicity testing; traditional medicine
Abstract:
... PURPOSE: Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated. METHODS: The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity usin ...
DOI:
10.1007/s00394-022-02900-4

16. 2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis

Author:
Mohammed W. Al-Rabia; Mohamed A. Alfaleh; Hani Z. Asfour; Waleed S. Alharbi; Mohamed A. El-Moselhy; Nabil A. Alhakamy; Usama A. Fahmy; Osama A. A. Ahmed; Omar Fahmy; Omar M. Rashad; Abdulmohsin J. Alamoudi; Ashraf B. Abdel-Naim
Source:
Antioxidants 2022 v.11 no.8 pp. -
ISSN:
2076-3921
Subject:
Western blotting; antioxidants; bioavailability; cyclosporine; kidneys; males; micelles; nanocarriers; nephrotoxicity; oral administration; particle size; polyethylene glycol; protein synthesis; succinic acid
Abstract:
... The immunosuppressant cyclosporine A (CSA) has been linked to serious renal toxic effects. Although 2-methoxyestradiol (2ME) possesses a wide range of pharmacological abilities, it suffers poor bioavailability after oral administration. The purpose of this study was to evaluate the potential of 2ME loaded D-ɑ-tocopheryl polyethylene glycol succinate (TPGS) micelles to prevent CSA-induced nephrotox ...
DOI:
10.3390/antiox11081499

17. 20 (S)-ginsenoside Rh2 inhibits colorectal cancer cell growth by suppressing the Axl signaling pathway in vitro and in vivo

Author:
Haibo Zhang; Jun-Koo Yi; Hai Huang; Sijun Park; Wookbong Kwon; Eungyung Kim; Soyoung Jang; Si-Yong Kim; Seong-kyoon Choi; Duhak Yoon; Sung-Hyun Kim; Kangdong Liu; Zigang Dong; Zae Young Ryoo; Myoung Ok Kim
Source:
Journal of Ginseng Research 2022 v.46 no.3 pp. 396-407
ISSN:
1226-8453
Subject:
Panax; Western blotting; antineoplastic activity; apoptosis; cell cycle checkpoints; cell growth; cell proliferation; colorectal neoplasms; morbidity; mortality; neoplasm cells; research; toxicity; transfection; xenotransplantation
Abstract:
... Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenoside Rh2 (G-Rh2) is a natural compound extracted from ginseng, which exhibits anticancer effects in many cancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo. Cell proliferation, migration, invasion, apoptosis, cell cycle, and western ...
DOI:
10.1016/j.jgr.2021.07.004

18. 3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

Author:
Jiajun Liu; Wen Hu; Xiaoying Ma; Xin Liang; Long Lin; Jianming Huang; Jianwen Liu
Source:
Environmental toxicology 2022 v.37 no.2 pp. 349-361
ISSN:
1520-4081
Subject:
Azolla; Western blotting; agonists; apoptosis; caspases; cell lines; cytotoxicity; digestive system; ecotoxicology; epithelial cells; immune system; membrane potential; mitochondria; radioprotective effect; radiotherapy
Abstract:
... Ionizing radiation (IR) brings many health problems to humans, causing damage to the digestive system, hematopoietic system, and immune system. Natural compounds derived from plants have attracted widespread attention due to their low toxicity. Here, we found that 3,4,5‐O‐tricaffeoylquinic acid (tCQA) extracted from natural plant Azolla imbricata could significantly alleviate the systemic damage i ...
DOI:
10.1002/tox.23403

19. 3,4-Dihydroxyphenylacetic acid ameliorates gut barrier dysfunction via regulation of MAPK-MLCK pathway in type 2 diabetes mice

Author:
Min Liu; Li Wang; Bijun Huang; Qun Lu; Rui Liu
Source:
Life sciences 2022 v.305 pp. 120742
ISSN:
0024-3205
Subject:
Western blotting; absorption barrier; anti-inflammatory activity; blood glucose; body weight; digestive system diseases; glutathione; high fat diet; hyperglycemia; inflammation; insulin; insulin resistance; interleukin-10; intestines; lipopolysaccharides; malondialdehyde; mitogen-activated protein kinase; myosin; noninsulin-dependent diabetes mellitus; oxidative stress; protein synthesis; streptozotocin; superoxide dismutase; tight junctions
Abstract:
... This study aims to investigate whether 3,4-dihydroxyphenylacetic acid (DHAA) can improve gut barrier function by inhibiting the mitogen-activated protein kinase (MAPK) - myosin light-chain kinase (MLCK) signaling pathway in type 2 diabetes (T2D) mice. T2D mice were induced by a high-fat diet combined with streptozotocin. T2D mice were intragastrically administered with DHAA at 75 and 150 mg kg/bod ...
DOI:
10.1016/j.lfs.2022.120742

20. IL- 37 alleviates liver granuloma caused by Schistosoma japonicum infection by inducing alternative macrophage activation

Author:
Cuiping Ren; Fengchun Liu; Chen Xing; Ruyu Zhao; Xiaoxue Tang; Miao Liu; Wenda Gao; Jijia Shen
Source:
Parasites & vectors 2022 v.15 no.1 pp. 300
ISSN:
1756-3305
Subject:
AMP-activated protein kinase; Schistosoma japonicum; Western blotting; blood serum; coculture; enzyme-linked immunosorbent assay; fibrosis; granuloma; interleukins; liver; liver cirrhosis; liver function; macrophage activation; macrophages; mice; people; phenotype; phosphorylation; reverse transcriptase polymerase chain reaction; schistosomiasis
Abstract:
... BACKGROUND: Hepatic macrophages regulate liver granuloma formation and fibrosis caused by infection with Schistosoma japonicum, with the manner of regulation dependent on macrophage activation state. Interleukin (IL)-37 may have immunomodulatory effects on macrophages. However, whether IL-37 can affect liver granuloma formation and fibrosis by affecting the polarization of macrophages in S. japoni ...
DOI:
10.1186/s13071-022-05420-6

21. 5‐Heptadecylresorcinol Protects against Atherosclerosis in Apolipoprotein E‐Deficient Mice by Modulating SIRT3 Signaling: The Possible Beneficial Effects of Whole Grain Consumption

Author:
Yiming Hao; Zihui Yang; Qing Li; Ziyuan Wang; Jie Liu; Jing Wang
Source:
Molecular nutrition & food research 2022 v.66 no.9 pp. e2101114
ISSN:
1613-4125
Subject:
Western blotting; apoptosis; atherosclerosis; biomarkers; blood serum; cholesterol; fluorescent antibody technique; food research; grain consumption; health claims; heart; high fat diet; histology; ingredients; mitochondria; risk; triacylglycerols; whole grain foods
Abstract:
... SCOPE: Whole grain consumption has been proven to be inversely associated with the risk of cardiovascular diseases. As a biomarker for whole grain dietary intake, 5‐heptadecylresorcinol (AR‐C17) has attracted increased attention due to its potential health‐improving activity. However, the beneficial effect of AR‐C17 on atherosclerosis prevention and the underlying mechanism remain unclear. METHODS ...
DOI:
10.1002/mnfr.202101114

22. 6-OH-BDE-47 inhibited proliferation of skin fibroblasts from pygmy killer whale by inducing cell cycle arrest

Author:
Tong Li; Yajing Sun; Ying Zeng; Edmond Sanganyado; Bo Liang; Wenhua Liu
Source:
Science of the total environment 2022 v.807 pp. 150561
ISSN:
0048-9697
Subject:
Orcinus orca; Western blotting; aquatic food webs; bioaccumulation; biphenyl; cell cycle checkpoints; cell proliferation; cyclin-dependent kinase; cyclins; environment; fibroblasts; hydroxylation; interphase; toxicity; toxicology
Abstract:
... Hydroxylated polybrominated diphenyl ethers (OH-BDEs) are major transformation products of PBDEs that readily bioaccumulate in the marine food web. Although 6-OH-BDE-47 is frequently and abundantly detected in cetaceans, its potential toxic effects are largely unknown. We explored the toxicological pathways and mechanisms of OH-BDEs by exposing pygmy killer whale skin fibroblast cell lines (PKW-LW ...
DOI:
10.1016/j.scitotenv.2021.150561

23. 6-methoxydihydroavicine, the alkaloid extracted from Macleaya cordata (Willd.) R. Br. (Papaveraceae), triggers RIPK1/Caspase-dependent cell death in pancreatic cancer cells through the disruption of oxaloacetic acid metabolism and accumulation of reactive oxygen species

Author:
Nengfang Ma; Fugen Shangguan; Hongfei Zhou; Huimin Huang; Jun Lei; Jing An; Guihua Jin; Weiwei Zhuang; Shipeng Zhou; Shijia Wu; Hongping Xia; Hailong Yang; Linhua Lan
Source:
Phytomedicine 2022 v.102 pp. 154164
ISSN:
0944-7113
Subject:
Macleaya cordata; Western blotting; alkaloids; apoptosis; caspases; cell growth; energy metabolism; flow cytometry; glycolysis; homeostasis; mitochondria; mitophagy; neoplasm cells; oxaloacetic acid; pancreatic neoplasms; reactive oxygen species
Abstract:
... Many extracts and purified alkaloids of M. cordata (Papaveraceae family) have been reported to display promising anti-tumor effects by inhibiting cancer cell growth and inducing apoptosis in many cancer types. However, no evidence currently exists for anti-pancreatic cancer activity of alkaloids extracted from M. cordata, including a novel alkaloid named 6‑methoxy dihydrosphingosine (6-Methoxydihy ...
DOI:
10.1016/j.phymed.2022.154164

24. 7,8-Dihydroxyflavone attenuates the virulence of Staphylococcus aureus by inhibiting alpha-hemolysin

Author:
Nan Bian; Xiangqian Chen; Xinran Ren; Zishu Yu; Mengli Jin; Xiaoyu Chen; Chang Liu; Yanhe Luan; Lin Wei; Ying Chen; Wu Song; Yicheng Zhao; Bingmei Wang; Tao Jiang; Chi Zhang; Zunhua Shu; Xin Su; Li Wang
Source:
World journal of microbiology & biotechnology 2022 v.38 no.11 pp. 200
ISSN:
0959-3993
Subject:
Staphylococcus aureus; Western blotting; animals; biotechnology; cytotoxicity; genes; hemolysis; hospitals; microbiology; synergism; therapeutics; transcription factors; vancomycin; viability; virulence
Abstract:
... Staphylococcus aureus (S. aureus), a Gram-positive bacteria, is an incurable cause of hospital and community-acquired infections. Inhibition bacterial virulence is a viable strategy against S. aureus infections based on the multiple virulence factors secreted by S. aureus. Alpha-hemolysin (Hla) plays a crucial role in bacteria virulence without affecting bacterial viability. Here, we identified th ...
DOI:
10.1007/s11274-022-03378-2

25. ACSL4 promotes colorectal cancer and is a potential therapeutic target of emodin

Author:
Guoliang Dai; Dong Wang; Shitang Ma; Shengwei Hong; Kang Ding; Xiying Tan; Wenzheng Ju
Source:
Phytomedicine 2022 v.102 pp. 154149
ISSN:
0944-7113
Subject:
Rheum palmatum; Western blotting; antineoplastic activity; carcinogenesis; cell proliferation; colorectal neoplasms; data collection; databases; emodin; gene expression regulation; immunohistochemistry; ingredients; mice; mortality; secretion; therapeutics; vascular endothelial growth factor receptor-1; vascular endothelial growth factor receptor-2
Abstract:
... Colorectal cancer (CRC) is an important death-related disease in the world and new therapeutic strategies are urgently needed to reduce mortality. Several studies have demonstrated that emodin, the main ingredient of Rheum palmatum, fights cancer but its potential anti-tumor effect on CRC is still unknown. The present study is aimed to explore the potential anti-tumor effects of emodin against CRC ...
DOI:
10.1016/j.phymed.2022.154149

26. AICAR stimulates mitochondrial biogenesis and BCAA catabolic enzyme expression in C2C12 myotubes

Author:
Jason S. Hinkle; Caroline N. Rivera; Roger A. Vaughan
Source:
Biochimie 2022 v.195 pp. 77-85
ISSN:
0300-9084
Subject:
Western blotting; acidification; amino acids; biogenesis; blood; catabolism; gene expression; genes; glycolysis; insulin resistance; keto acids; metabolites; mitochondria; models; myotubes; noninsulin-dependent diabetes mellitus; oxidoreductases; oxygen consumption; peroxisome proliferator-activated receptor gamma; protein kinases; protein synthesis; skeletal muscle
Abstract:
... Type 2 diabetes is characterized by reduced insulin sensitivity, elevated blood metabolites, and reduced mitochondrial metabolism. Insulin resistant populations often exhibit reduced expression of genes governing mitochondrial metabolism such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α). Interestingly, PGC-1α regulates the expression of branched-chain amino acid ...
DOI:
10.1016/j.biochi.2021.11.004

27. ALDH6A1 weakens the progression of colon cancer via modulating the RAS/RAF/MEK/ERK pathway in cancer cell lines

Author:
Xiang Li; Nan Wang; Yutong Wu; Yidan Liu; Ruoyu Wang
Source:
Gene 2022 v.842 pp. 146757
ISSN:
0378-1119
Subject:
Western blotting; adenocarcinoma; aldehyde dehydrogenase; apoptosis; colon; colorectal neoplasms; fibrosarcoma; genes; metalloproteinases; neoplasm cells; pathogenesis; quantitative polymerase chain reaction; rats; transfection; viruses
Abstract:
... Aldehyde dehydrogenase 6 family member A1 (ALDH6A1) is associated with multiple diseases, but its pathogenesis in colon cancer (CC) is ambiguous and needs further study so that this research explores the function of ALDH6A1 in CC. The level of ALDH6A1 in colon adenocarcinoma (COAD), CC tissues, and cells was measured by starBase v2.0, quantitative real-time polymerase chain reaction (qRT-PCR), and ...
DOI:
10.1016/j.gene.2022.146757

28. ASPN Is a Potential Biomarker and Associated with Immune Infiltration in Endometriosis

Author:
Li Wang; Jing Sun
Source:
Genes 2022 v.13 no.8 pp. -
ISSN:
2073-4425
Subject:
B-lymphocytes; T-lymphocytes; Western blotting; biomarkers; calcium; chemokines; cytotoxicity; data collection; databases; endometriosis; endometrium; endothelial cells; extracellular matrix; female genital diseases; fibroblasts; gene expression; gene expression regulation; genes; immunotherapy; metastasis; muscle contraction; patients; regression analysis; smooth muscle
Abstract:
... Objective: Endometriosis is a benign gynecological disease characterized by distant metastasis. Previous studies have discovered abnormal numbers and function of immune cells in endometriotic lesions. We aimed to find potential biomarkers of endometriosis and to explore the relationship between ASPN and the immune microenvironment of endometriosis. Methods: We obtained the GSE141549 and GSE7305 da ...
DOI:
10.3390/genes13081352

29. ATP6V1H deficiency impairs glucose tolerance by augmenting endoplasmic reticulum stress in high fat diet fed mice

Author:
Shaoqing Yang; Yuzhuan Hou; Hengwei Zhang; Ying Hao; Yanli Zhang; Zanyan Zhao; Wenyan Ruan; Xiaohong Duan
Source:
Archives of biochemistry and biophysics 2022 v.716 pp. 109116
ISSN:
0003-9861
Subject:
H-transporting ATP synthase; Western blotting; biophysics; diabetes; endoplasmic reticulum stress; glucose; glucose tolerance; high fat diet; insulin; insulin secretion; models; proton pump; transcriptome; transportation; vacuoles
Abstract:
... Vacuolar H⁺-ATPase (V-ATPase) is a ubiquitous proton pump that mediates the proton transmembrane transportation in various cells. Previously, H subunit of V-ATPase (ATP6V1H) was found to be related with insulin secretion and diabetes. However, the mechanism by which ATP6V1H regulates insulin secretion and glucose metabolism remains unclear. Herein, we established a high-fat-diet (HFD) fed model wi ...
DOI:
10.1016/j.abb.2022.109116

30. Abnormal Expression of microRNA-296-3p in Type 2 Diabetes Patients and its Role in Pancreatic β-Cells Function by Targeting Tensin Homolog Deleted on Chromosome Ten

Author:
Minggang Cheng; Yichen Guo; Weichuan Zhong; Xueyan Chen; Guangzhou Guo
Source:
Biochemical genetics 2022 v.60 no.1 pp. 39-53
ISSN:
0006-2928
Subject:
Western blotting; apoptosis; blood glucose; blood serum; cell proliferation; cell viability; chromosomes; flow cytometry; insulin; insulin secretion; noninsulin-dependent diabetes mellitus; protective effect; reverse transcriptase polymerase chain reaction; therapeutics; transfection; uric acid
Abstract:
... Diabetes mellitus (DM), a familiar disease, is characterized by high blood glucose levels owing to insulin deficiency. Researches have suggested that the incidence rate of diabetes is increasing and it has become an important global epidemic. The type 2 diabetes mellitus (T2DM) is featured with pancreatic β-cell loss and lack of insulin release. Nevertheless, the therapeutic methods that was helpf ...
DOI:
10.1007/s10528-021-10083-6

31. Activation of mitochondrial-associated apoptosis signaling pathway and inhibition of PI3K/Akt/mTOR signaling pathway by voacamine suppress breast cancer progression

Author:
Yi Zuo; Chao-zheng Zhang; Qing Ren; Yao Chen; Xiao Li; Ji-rui Yang; Hong-xiang Li; Wen-tao Tang; Hing-Man HO; Chen Sun; Mei-mei Li; Bo Ren; Yun Deng; Mao-lin Wang; Jun Lu
Source:
Phytomedicine 2022 v.99 pp. 154015
ISSN:
0944-7113
Subject:
Voacanga africana; Western blotting; alkaloids; apoptosis; breast neoplasms; cell proliferation; cyclin-dependent kinase; cyclins; flow cytometry; humans; interphase; membrane potential; mice; mitochondrial membrane; morbidity; mortality; neoplasm cells; neoplasm progression; protein synthesis; reactive oxygen species; toxicity
Abstract:
... Breast cancer is one of the malignant tumors with the highest morbidity and mortality rate. Numerous efficient anti-breast cancer drugs are being derived from the development of natural products. Voacamine (VOA), a bisindole alkaloid isolated from Voacanga africana Stapf, possesses various pharmacological and biological activities. In this study, we investigated the efficacy of VOA against breast ...
DOI:
10.1016/j.phymed.2022.154015

32. Active legumain promotes invasion and migration of neuroblastoma by regulating epithelial-mesenchymal transition

Author:
Min Zhang; Jianhua Zhu; Wei Wang; Zhiteng Jiang
Source:
Open life sciences 2022 v.17 no.1 pp. 676-685
ISSN:
2391-5412
Subject:
Western blotting; asparagine; cadherins; gene amplification; gene targeting; genes; humans; legumain; metastasis; neoplasms; slugs; transfection; vimentin
Abstract:
... Neuroblastoma (NB) is a commonly occurring malignancy in children. Epithelial-mesenchymal transition (EMT) is an adaptive change in promoting tumor metastasis. As an important factor in regulating tumor metastasis, whether legumain could promote metastasis of NB by EMT is still unexplored. Legumain is the active form of prolegumain, abundant in tumor plasma. So in the current study, different form ...
DOI:
10.1515/biol-2022-0012

33. Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells

Author:
Lingli Chen; Yuye Liu; Penghuan Jia; Hongli Zhang; Zhihong Yin; Dongfang Hu; Hongmei Ning; Yaming Ge
Source:
Environmental science and pollution research 2022 v.29 no.39 pp. 58927-58935
ISSN:
0944-1344
Subject:
Western blotting; axons; cell membranes; cerebral cortex; dose response; females; heavy metals; hippocampus; lead acetate; memory; mice; microscopy; nerve tissue; neurotoxicity; permeability; pollution; protein synthesis; research; survival rate
Abstract:
... Lead (Pb) is a widespread environmental heavy metal that can damage the cerebral cortex and hippocampus, and reduce the learning and memory ability in humans and animals. In vivo and in vitro models of acute lead acetate exposure were established to further study the mechanism of neurons injury. In this study, 4-week-old female Kunming mice were randomly divided into four groups. Each group was tr ...
DOI:
10.1007/s11356-022-20051-1

34. Aerobic exercise improves adipogenesis in diet-induced obese mice via the lncSRA/p38/JNK/PPARγ pathway

Author:
Baoai Wu; Jiyuan Ding; Anping Chen; Yicun Song; Cong Xu; Fujun Tian; Jinfeng Zhao
Source:
Nutrition research 2022 v.105 pp. 20-32
ISSN:
0271-5317
Subject:
Western blotting; adipogenesis; adiposity; blood lipids; epididymis; exercise; lipid metabolism; lipids; mitogen-activated protein kinase; nutrition research; obesity; risk factors; spectrophotometers; viruses; white adipose tissue
Abstract:
... Adipogenesis is one of the triggers of obesity, which is a risk factor for various metabolic diseases. Long noncoding RNA steroid receptor RNA activator (lncRNA-SRA) is closely related to adipogenesis and p38/JNK mitogen-activated protein kinase mediates lipid production by regulating peroxisome proliferator-activated receptor gamma (PPARγ). Aerobic exercise can be efficient in improving adiposity ...
DOI:
10.1016/j.nutres.2022.04.004

35. Aesculetin exhibited anti-inflammatory activities through inhibiting NF-кB and MAPKs pathway in vitro and in vivo

Author:
Shou-Kai Wang; Ting-Xiao Chen; Wei Wang; Ling-Ling Xu; Yu-Qing Zhang; Zhen Jin; You-Bin Liu; You-Zhi Tang
Source:
Journal of ethnopharmacology 2022 v.296 pp. 115489
ISSN:
0378-8741
Subject:
Western blotting; anti-inflammatory activity; anti-inflammatory agents; colitis; colon; interleukin-6; macrophages; mice; secretion; traditional medicine; weight loss
Abstract:
... Aesculetin (6,7-dihydroxy-2H-1-benzopyran-2-one) has been reported to exhibit potent anti-inflammatory property both in vitro and in vivo. In this study, we evaluated the anti-inflammatory effect and investigated underlying molecular mechanisms of aesculetin in LPS-induced RAW264.7 macrophages and DSS-induced colitis. In this study, the production of NO, TNF-α, and IL-6 were measured to identify t ...
DOI:
10.1016/j.jep.2022.115489

36. Albumin (ALB) and protein disulfide isomerase family A member 4 (PDIA4) are novel markers to predict sperm freezability of Erhualian boar

Author:
Shifeng Tong; Chang Yin; Yuzhu Ge; Zhibin Ren; Jingli Tao; Yang Liu
Source:
Cryobiology 2022 pp. -
ISSN:
0011-2240
Subject:
Western blotting; albumins; boars; cold tolerance; cryobiology; fluorescent antibody technique; freeze-thaw cycles; protein disulfide-isomerase; proteomics; semen quality; sperm quality
Abstract:
... The frozen semen of Erhualian pig can promote the continuous improvement of commercial pigs, but currently, frozen semen fails to satisfy the practical application requirement. Oxidative damage is one of the crucial factors affecting the quality of frozen semen; besides, there are individual differences in boar sperm freezability. Based on the previous analysis of the proteomic differences of Erhu ...
DOI:
10.1016/j.cryobiol.2022.09.006

37. All-trans retinoic acid-mediated miR-30a up-regulation suppresses autophagy and sensitizes gastric cancer cells to cisplatin

Author:
Asadollah Abbasi; Mohammadali Hosseinpourfeizi; Reza Safaralizadeh
Source:
Life sciences 2022 v.307 pp. 120884
ISSN:
0024-3205
Subject:
Western blotting; apoptosis; autophagy; cell cycle checkpoints; cisplatin; culture media; drug therapy; flow cytometry; genes; microRNA; retinoic acid; stem cells; stomach neoplasms; toxicity testing
Abstract:
... The potential of all-trans retinoic acid (ATRA) in regulating some microRNAs (miRNAs) involved in multiple cancer-related pathways, including resistance to chemotherapeutics, may be a valuable idea for overcoming the CDDP resistance of GC cells. Treatment of gastric AGS and MKN-45 cells with CDDP enriched the CDDP surviving cells (CDDP-SCs). The abilities of chemoresistance to CDDP drug, migration ...
DOI:
10.1016/j.lfs.2022.120884

38. Alleviating Effects of Black Soybean Peptide on Oxidative Stress Injury Induced by Lead in PC12 Cells via Keap1/Nrf2/TXNIP Signaling Pathway

Author:
Ning Li; Liuding Wen; Tiange Li; Huijie Yang; Mingwu Qiao; Tianlin Wang; Lianjun Song; Xianqing Huang; Mingming Li; Erkigul Bukyei; Fangyu Wang
Source:
Nutrients 2022 v.14 no.15 pp. -
ISSN:
2072-6643
Subject:
Western blotting; cell viability; lead; oxidative stress; peptides; protein synthesis; soybeans
Abstract:
... Many researchers have found that Pb exposure can cause oxidative stress damage to the body’s tissue. Black soybean peptide (BSP) has a variety of physiological functions, especially in terms of oxidative stress. Nevertheless, the mitigation function of BSPs on Pb-induced oxidative stress damage in PC12 cells has not been clearly defined. In this study, cell viability was detected by CCK8. Oxidativ ...
DOI:
10.3390/nu14153102

39. Allicin attenuated hepatic ischemia/reperfusion injury in mice by regulating PPARγ-IRAK-M-TLR4 signal pathway

Author:
Weiwei Li; Rui Huang; Xia Gong; Zizuo Zhao; Lidan Zhang; Qin Zhou; Xujie Jiang; Hongtao Tie; Jingyuan Wan; Bin Wang
Source:
Food & function 2022 v.13 no.13 pp. 7361-7376
ISSN:
2042-650X
Subject:
Western blotting; allicin; apoptosis; blood; dose response; drugs; garlic; histology; ischemia; liver; liver function; mechanism of action; models; morbidity; mortality; necrosis; neoplasms; oxidative stress; peroxisome proliferator-activated receptor gamma; protective effect; reperfusion injury; signal transduction; surgery
Abstract:
... Background: Hepatic ischemia/reperfusion (I/R) injury to the liver is a significant cause of morbidity and mortality following liver surgery, trauma, and hemorrhagic shock. It was reported that allicin, a type of garlic compound, had a protective effect against other hepatic diseases. Allicin's ability to protect against liver injury caused by ischemic reperfusion remains unknown. As a result, we ...
DOI:
10.1039/d2fo00751g

40. Alpha-linolenic acid inhibits hepatocellular carcinoma cell growth through Farnesoid X receptor/β-catenin signaling pathway

Author:
Shu Feng; Xingming Xie; Chaochun Chen; Shi Zuo; Xueke Zhao; Haiyang Li
Source:
Nutrition & metabolism 2022 v.19 no.1 pp. 57
ISSN:
1743-7075
Subject:
Western blotting; acid treatment; alpha-linolenic acid; cell growth; gas chromatography; hepatoma; lipid composition; migratory behavior; neoplasm cells; nutrition; protein synthesis; spectrometers
Abstract:
... BACKGROUND: Altered lipid profiles are frequently present in cancer, and it is necessary to elucidate the role of changed lipid profiles in hepatocellular carcinoma (HCC). We conducted this study to investigate the changed lipid profile in HCC tissues and discover some remarkably changed lipid components, and to explore the function of changed lipid components in HCC development. METHODS: Gas chro ...
DOI:
10.1186/s12986-022-00693-1

41. Alteration of LncRNA expression in mice placentae after frozen embryo transfer is associated with increased fetal weight

Author:
Yuan Zhu; Yunni Cai; Bo Zheng; Min Ding; Yuting Zhang; Jianjun Zhou
Source:
Reproductive Biology 2022 v.22 no.2 pp. 100646
ISSN:
1642-431X
Subject:
Western blotting; birth weight; blastocyst; embryo transfer; mice; microRNA; morphogenesis; sequence analysis; superovulation
Abstract:
... The birthweight after frozen embryo transfer (FET) was significantly higher compared with fresh embryo transfer (fresh ET), while the mechanism remains unclear. In this study, we transferred vitrified-warmed or fresh mice blastocysts into pseudopregnant recipients (n = 11 each group) produced by natural mating to avoid the influence of superovulation. The fetal weight, placental weight, placental ...
DOI:
10.1016/j.repbio.2022.100646

42. The Alterations and Significance of Intercellular Adhesion Molecule-1 in Mouse Brainstem During Herpes Simplex Virus Type 1–Induced Facial Palsy

Author:
Hong Mu; Junjie Liu; Yanyan Mao; Yuechen Han; Lei Xu; Daogong Zhang; Haibo Wang
Source:
Applied biochemistry and biotechnology 2022 v.194 no.8 pp. 3483-3493
ISSN:
0273-2289
Subject:
Human alphaherpesvirus 1; Western blotting; animal models; biotechnology; brain stem; face; genes; glucocorticoids; herpes simplex; intercellular adhesion molecule-1; males; methylprednisolone; mice; nerve tissue; paralysis; pathophysiology; protein synthesis; quantitative polymerase chain reaction; viruses
Abstract:
... Herpes simplex virus type 1 (HSV-1) results in the development of Bell’s pals but still, the pathophysiology of the facial nerve paralysis is still not fully studied. The main objective is to establish an animal model of type 1 herpes simplex virus (HSV-1)–induced face paralysis in the mouse and to investigate the pattern of changes in intercellular adhesion molecule -1(ICAM-1) expression in the f ...
DOI:
10.1007/s12010-022-03837-4

43. Altered m6A RNA methylation contributes to hippocampal memory deficits in Huntington’s disease mice

Author:
Anika Pupak; Ankita Singh; Anna Sancho-Balsells; Rafael Alcalá-Vida; Marc Espina; Albert Giralt; Eulàlia Martí; Ulf Andersson Vang Ørom; Silvia Ginés; Verónica Brito
Source:
Cellular and molecular life sciences 2022 v.79 no.8 pp. 416
ISSN:
1420-682X
Subject:
RNA; Western blotting; cognition; demethylation; fractionation; hippocampus; memory; methylation; methyltransferases; precipitin tests; synapse; transcriptome
Abstract:
... N6-methyladenosine (m6A) regulates many aspects of RNA metabolism and is involved in learning and memory processes. Yet, the impact of a dysregulation of post-transcriptional m6A editing on synaptic impairments in neurodegenerative disorders remains unknown. Here we investigated the m6A methylation pattern in the hippocampus of Huntington’s disease (HD) mice and the potential role of the m6A RNA m ...
DOI:
10.1007/s00018-022-04444-6

44. Amelioration of lipid accumulations and metabolism disorders in differentiation and development of 3T3-L1 adipocytes through mulberry leaf water extract

Author:
Yan Du; Ding-xiang Li; Dong-yu Lu; Ran Zhang; Qiao-qiao Zhong; Yan-lin Zhao; Xiao-xiao Zheng; Shuai Ji; Liang Wang; Dao-Quan Tang
Source:
Phytomedicine 2022 v.98 pp. 153959
ISSN:
0944-7113
Subject:
AMP-activated protein kinase; Oriental traditional medicine; Western blotting; acetyl-CoA carboxylase; adipocytes; adiponectin; adiponectin receptors; adipose tissue; arachidonic acid; biosynthesis; carboxylic ester hydrolases; fatty-acid synthase; glycerol; glycerophospholipids; high performance liquid chromatography; inflammation; interleukin-10; interleukin-6; leaves; metabolites; metabolomics; molecular biology; mulberries; obesity; quantitative polymerase chain reaction; sterols; tandem mass spectrometry
Abstract:
... Obesity is a worldwide problem that resulted from the excessive fat accumulation in adipose tissue, leading to the impairment of individual health. Mulberry leaf is an important traditional Chinese medicine and has been used to alleviate obesity for a long term. However, its underlying molecular mechanisms have not been fully elucidated yet. In this study, we aimed to investigate the inhibition ef ...
DOI:
10.1016/j.phymed.2022.153959

45. Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis

Author:
Rongchun Wang; Qingyu Ren; Daili Gao; Yam Nath Paudel; Xia Li; Lizhen Wang; Pengyu Zhang; Baokun Wang; Xueliang Shang; Meng Jin
Source:
Journal of ethnopharmacology 2022 v.289 pp. 115018
ISSN:
0378-8741
Subject:
Danio rerio; Gastrodia elata; Oriental traditional medicine; Western blotting; adults; antidepressants; apoptosis; cell viability; diet; fluorescent antibody technique; gene expression; models; pharmacology; protein synthesis; China
Abstract:
... Gastrodia elata Blume (G. elata), a traditional Chinese herb, known as “Tian Ma”, is widely used as a common medicine and diet ingredient for treating or preventing neurological disorders for thousands of years in China. However, the anti-depressant effect of G. elata and the underlying mechanism have not been fully evaluated. The study is aimed to investigate the anti-depressant effect and the mo ...
DOI:
10.1016/j.jep.2022.115018

46. Ameliorative effect of the ethnomedicinal plant Blepharis maderaspatensis (L.) B.Heyne ex Roth on lipopolysaccharide-induced acute liver inflammation and oxidative stress in Wistar rat model

Author:
Thadiyan Parambil Ijinu; Kuttapetty Manikantan; Ajayakumar Lalitha Aneeshkumar; Vijayan Soorya; Panickamparambil Gopalakrishnan Latha; Varughese George; Palpu Pushpangadan
Source:
Phytomedicine plus 2022 v.2 no.2 pp. 100247
ISSN:
2667-0313
Subject:
Blepharis; Western blotting; acute oral toxicity; albino; aluminum chloride; animal models; antioxidants; blood serum; body weight; drugs; gene expression; guidelines; hematology; histopathology; inflammation; interleukin-6; liver; medicine; oxidative stress; phosphorylation; protective effect; silymarin; thin layer chromatography; India
Abstract:
... Blepharis maderaspatensis is an ethnomedicinal plant used by the Mavilan and Koraga tribes of Kerala state, India for the treatment of liver diseases. Thus, the present study aims to evaluate the liver protective activity of defatted ethanolic extract of B. maderaspatensis on lipopolysaccharide-induced acute liver inflammation and oxidative stress in Wistar rat model. Preliminary phytochemical eva ...
DOI:
10.1016/j.phyplu.2022.100247

47. Amentoflavone impairs the reconsolidated fear memories through inhibition of ERK pathway

Author:
Kuisheng Sun; Ruizhu Xie; Zhengjun Yi; Liqian Yin; Yan Guan; Lujuan Wang; Ying Wang; Xiaoyan Ding; Yaning Luan; Huijie Liu; Yuhan Fan
Source:
Biochemical and biophysical research communications 2022 v.622 pp. 8-14
ISSN:
0006-291X
Subject:
Western blotting; amygdala; animal behavior; fearfulness; inflammation; memory; pathophysiology; research
Abstract:
... Post-traumatic stress disorder (PTSD) is a pathological fear memory-related disease. The persistence of pathological fearful memories is one of the most characteristic symptoms of PTSD. However, this can be eliminated by intervening in reconsolidation. Inflammation is intimately involved in the pathophysiologic progression of PTSD. Amentoflavone (AF) has anti-inflammatory effects. However, the eff ...
DOI:
10.1016/j.bbrc.2022.07.024

48. Ammonia-induced oxidative stress triggered proinflammatory response and apoptosis in pig lungs

Author:
Daojie Li; Long Shen; Di Zhang; Xiaotong Wang; Qiankun Wang; Wenhao Qin; Yun Gao; Xiaoping Li
Source:
Journal of environmental sciences (China) 2023 v.126 pp. 683-696
ISSN:
1001-0742
Subject:
DNA; Western blotting; ammonia; animal models; apoptosis; dose response; epithelium; genes; inflammation; lungs; metabolome; mineral fertilizers; oxidative phosphorylation; oxidative stress; protein synthesis; pyruvic acid; swine; toxicity; transcriptome; transmission electron microscopy; China
Abstract:
... Ammonia, a common toxic gas, is not only one of the main causes of haze, but also can enter respiratory tract and directly affect the health of humans and animals. Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung. In this study, the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using trans ...
DOI:
10.1016/j.jes.2022.05.005

49. Analysis of the resistance of small peptides from Periplaneta americana to hydrogen peroxide-induced apoptosis in human ovarian granular cells based on RNA-seq

Author:
Qin Wang; Rong Fu; Honghan Cheng; Yi Li; Shiyan Sui
Source:
Gene 2022 v.813 pp. 146120
ISSN:
0378-1119
Subject:
4',6-diamidino-2-phenylindole; Periplaneta americana; RNA transport; Western blotting; apoptosis; caspase-3; fluorescence; gene expression regulation; genes; humans; hydrogen peroxide; interleukin-17; leukocytes; quantitative polymerase chain reaction; sequence analysis; transcriptome
Abstract:
... Apoptosis of ovarian granular cells is closely related with weakening fertility of women. Hence, resisting apoptosis of human ovarian granular cells is of important significance. According to previous studies, DAPI fluorescence staining experiment and Western Blot test of Caspase-3 demonstrate that small peptides from Periplaneta americana (SPPA) can improve hydrogen peroxide (H₂O₂) -induced apopt ...
DOI:
10.1016/j.gene.2021.146120

50. Anemoside B4 prevents chronic obstructive pulmonary disease through alleviating cigarette smoke-induced inflammatory response and airway epithelial hyperplasia

Author:
Huimiao Ma; Ziye Zhou; Lanying Chen; Lingling Wang; Qi Muge
Source:
Phytomedicine 2022 v.107 pp. 154431
ISSN:
0944-7113
Subject:
Anemone chinensis; Western blotting; catalase; cell viability; cigarettes; epithelium; glutathione peroxidase; humans; hyperplasia; inflammation; ingredients; interleukin-8; lung function; lung neoplasms; lungs; malondialdehyde; medicinal plants; mice; models; oxidative stress; protein synthesis; proteinases; reactive oxygen species; risk; smoke; stress response; superoxide dismutase; therapeutics
Abstract:
... Cigarette smoke (CS) is one of the major risk factors for chronic obstructive pulmonary disease (COPD) and increases the risk of lung cancer (LC). Anemoside B4 (B4) is the main bioactive ingredient in Pulsatilla chinensis (P. chinensis), a traditional medicinal herb for various diseases. It has a wide range of anti-inflammatory, anti-oxidation and anti-cancer activities. However, in recent years, ...
DOI:
10.1016/j.phymed.2022.154431

51. Angiotensin II can trigger HSC-LX2 pyroptosis through both classical and non-classical pathways

Author:
Ze-yu Xie; Yi-xiao Xu; Li Yao
Source:
Life sciences 2022 v.307 pp. 120878
ISSN:
0024-3205
Subject:
Western blotting; cell proliferation; cell viability; gene expression; humans; interleukin-18; liver cirrhosis; pyroptosis; therapeutics
Abstract:
... Current evidence suggests that liver fibrosis is reversible even at late stages. Pyroptosis is reportedly regulated by classical and non-classical pathways and is also involved in the activation of the human hepatic stellate cell line LX2. This study sought to identify regulatory pathways that pyroptosis of HSC during AngII-ROS-induced HSC activation and provides novel insights for anti-fibrosis t ...
DOI:
10.1016/j.lfs.2022.120878

52. Angiotensin-(1–7) promotes mitochondrial translocation of human telomerase reverse transcriptase in HUVECs through the TOM20 complex

Author:
Weikai Hu; Yinan Guo; Xueyan Wang; Tianyu Cui; Chunyun Li; Ruixia Liu; Chenghong Yin
Source:
Archives of biochemistry and biophysics 2022 v.722 pp. 109218
ISSN:
0003-9861
Subject:
Western blotting; antagonists; biophysics; fluorescent antibody technique; gene expression; humans; inflammation; lipopolysaccharides; mitochondria; models; peptides; protein content; protein synthesis; reverse transcriptase polymerase chain reaction; telomerase; transfection
Abstract:
... Angiotensin (Ang) (1–7) is a vasodilator peptide that ameliorates microcirculation dysfunction, increases telomerase activity in cells, and exerts vasodilatory, anti-inflammatory, antioxidative stress, and antiapoptotic effects. Mitochondrial human telomerase reverse transcriptase (hTERT) plays an important role in the processes of antiapoptosis, antioxidative stress, and immortalization. This stu ...
DOI:
10.1016/j.abb.2022.109218

53. Annual changes in cell proliferation and apoptosis and expression of connexin 43 in the testes of domestic seasonal breeding ganders

Author:
Małgorzata Gumułka; Anna Hrabia; Israel Rozenboim
Source:
Theriogenology 2022 v.186 pp. 27-39
ISSN:
0093-691X
Subject:
Western blotting; animal reproduction; apoptosis; caspase-3; cell proliferation; connexins; epithelium; fluorometry; ganders; gap junctions; gene expression; genes; histology; morphometry; testes
Abstract:
... Molecular mechanisms of seasonal changes in testicular morphology, histoarchitecture, and functions in birds have not been fully elucidated. The aim of the study was to examine the alternations in cell proliferation and apoptosis, these processes-related gene expressions, and gap junction protein (GJA1gene, connexin 43 protein; Cx43) expression and localization in gander testes during annual stage ...
DOI:
10.1016/j.theriogenology.2022.03.031

54. Antagonistic Effect of Selenium on Fumonisin B1 Promotes Neutrophil Extracellular Traps Formation in Chicken Neutrophils

Author:
Huquan Zhu; Qinfang Yu; Huimin Ouyang; Ruofan Zhang; Jinhong Li; Runxi Xian; Kai Wang; Xinran Li; Changyu Cao
Source:
Journal of agricultural and food chemistry 2022 v.70 no.19 pp. 5911-5920
ISSN:
1520-5118
Subject:
DNA; Western blotting; chickens; drugs; elastase; fluorescence; fluorescence microscopy; food chemistry; fumonisin B1; gene expression; genes; glutathione peroxidase; histones; innate immunity; mechanism of action; neutrophils; protein synthesis; quantitative polymerase chain reaction; reactive oxygen species; selenoproteins; thioredoxins; toxicity
Abstract:
... Neutrophils are an important component of the innate immune system, and one of their defense mechanisms, neutrophil extracellular traps (NETs), is a hot topic of the current research. This study explored the effects of fumonisin B1 (FB1) on chicken neutrophil production of NETs and its possible molecular mechanism of action. Scanning electron microscopy and fluorescence microscopy were used to obs ...
DOI:
10.1021/acs.jafc.2c01329

55. Antagonistic effects of selenium on lead-induced oxidative stress and apoptosis of Leydig cells in sheep

Author:
Lei Shi; Xiaolei Wang; Yunli Duan; Kexin Li; Youshe Ren
Source:
Theriogenology 2022 v.185 pp. 43-49
ISSN:
0093-691X
Subject:
Western blotting; animal reproduction; apoptosis; caspase-3; caspase-8; cell proliferation; cell viability; gene expression; lead; oxidative stress; quantitative polymerase chain reaction; selenium; sheep
Abstract:
... The objective of this study was to investigate the antagonistic effects of selenium (Se) on lead (Pb)-induced oxidative stress and apoptosis of sheep Leydig cells and its underlying mechanism. Leydig cells collected from 8-month-old sheep were treated with Pb (40 μmol/L) and/or Se (2 μmol/L), respectively. CCK-8 assay was used to detect cell proliferation and apoptosis after cultured for 48 h. The ...
DOI:
10.1016/j.theriogenology.2022.03.023

56. Antarctic krill peptides improve scopolamine-induced memory impairment in mice

Author:
Jingru Zheng; Yuanhong Gao; Jie Ding; Na Sun; Songyi Lin
Source:
Food bioscience 2022 v.49 pp. 101987
ISSN:
2212-4292
Subject:
Euphausia superba; Western blotting; blood serum; caspase-3; memory disorders; mice; peptides; scopolamine
Abstract:
... The study aims to investigate the repair effects of Ser-Ser-Asp-Ala-Phe-Phe-Pro-Phe-Arg (SSDAFFPFR) and Ser-Asn-Val-Phe-Asp-Met-Phe (SNVFDMF) peptides on scopolamine-caused memory impairment in mice. Behavioral experiments have revealed that Antarctic krill peptides can ameliorate scopolamine caused memory impairment by changing the behavior of mice. In comparison with the scopolamine group, the t ...
DOI:
10.1016/j.fbio.2022.101987

57. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium

Author:
Masahiro Terasawa; Keiichi Hiramoto; Ryota Uchida; Koji Suzuki
Source:
Marine drugs 2022 v.20 no.2 pp. -
ISSN:
1660-3397
Subject:
Monostroma; Western blotting; anti-inflammatory activity; blood circulation; caudal vein; endothelium; humans; immunohistochemistry; inflammation; jejunum; lipopolysaccharides; liver; lungs; mice; neutrophils; sulfates; syndecans
Abstract:
... We previously reported that rhamnan sulfate (RS) purified from Monostroma nitidum significantly suppressed lipopolysaccharide (LPS)-induced inflammation in cultured human vascular endothelial cells. Here, we analyzed the effect of orally administered RS on LPS-induced damage to mouse organs and vascular endothelium. RS (1 mg) was orally administered daily to BALB/c mice, 50 μg of LPS was intraperi ...
DOI:
10.3390/md20020121

58. Anti-L1 antibody-bound HPV16 pseudovirus is degraded intracellularly via TRIM21/proteasomal pathway

Author:
Meiying Li; Jianmei Huang; Yi Zhu; Ziyi Huang; Guonan Zhang; Jianming Huang
Source:
Virology journal 2022 v.19 no.1 pp. 90
ISSN:
1743-422X
Subject:
Pseudovirus; Western blotting; antibodies; bioluminescence assay; coat proteins; cytokines; cytosol; epithelium; flow cytometry; fluorescence microscopy; plasmids; proteasome endopeptidase complex; risk factors; transfection; uterine cervical neoplasms; virology; viruses
Abstract:
... BACKGROUND: Persistent HPV16 infection is the leading risk factor for developing cervical cancer. Anti-L1 antibodies against HPV16 produced in HPV16 infections play diverse roles in the clearance of virus infection and prevention of persistence. It has been implicated that the cervicovaginal squamous epithelial cells actually express TRIM21 and that some HPV16 particles could escape leaky endosoma ...
DOI:
10.1186/s12985-022-01826-x

59. Anti-Parkinsonian effect of Mucuna pruriens and Ursolic acid on GSK3β/Calcium signaling in neuroprotection against Rotenone-induced Parkinsonism

Author:
Walia Zahra; Hareram Birla; Saumitra Sen Singh; Aaina Singh Rathore; Hagera Dilnashin; Richa Singh; Priyanka Kumari Keshri; Shekhar Singh; Surya Pratap Singh
Source:
Phytomedicine plus 2022 v.2 no.4 pp. 100343
ISSN:
2667-0313
Subject:
Mucuna pruriens; Parkinson disease; Western blotting; apoptosis; calcium; death; eukaryotic cells; glycogen (starch) synthase; homeostasis; immunohistochemistry; mice; mitochondria; models; neuroprotective effect; oxidative stress; pathogenesis; protein kinases; protein synthesis; rotenone; serine; threonine; toxins; ursolic acid
Abstract:
... Exposure of environmental toxin is linked to the onset of Parkinson's disease (PD), especially in majority of sporadic cases. One of such toxins, namely Rotenone has been widely investigated to produce the Parkinsonian model in laboratories. The toxin causes the death of the dopaminergic neurons, aggregation of α-Synuclein, oxidative stress, neuroinflammation and initiate other pathways leading to ...
DOI:
10.1016/j.phyplu.2022.100343

60. Anti-infection roles of miR-155-5p packaged in exosomes secreted by dendritic cells infected with Toxoplasma gondii

Author:
Dan Jiang; Shuizhen Wu; Liqing Xu; Guantai Xie; Dongliang Li; Hongjuan Peng
Source:
Parasites & vectors 2022 v.15 no.1 pp. 3
ISSN:
1756-3305
Subject:
Protozoa; Toxoplasma gondii; Western blotting; cell communication; exosomes; genes; interleukin-6; macrophages; mice; pregnancy; secretion; tachyzoites; toxoplasmosis; transmission electron microscopy; ultracentrifugation
Abstract:
... BACKGROUND: Toxoplasma gondii is a zoonotic intracellular protozoon that is estimated to infect about 30% of the world’s population, resulting in toxoplasmosis in immunocompromised patients and adverse outcomes in cases of primary infection during pregnancy. Exosomes are tubular vesicles secreted by cells, and function in intercellular communication. It has been reported that the exosomes secreted ...
DOI:
10.1186/s13071-021-05003-x

61. Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice

Author:
Zhuohong Li; Ying Gao; Lei Du; Ye Yuan; Wenbo Huang; Xi Fu; Yongliang Huang; Xufan Zhang; Fengming You; Shijie Li
Source:
Journal of ethnopharmacology 2022 v.292 pp. 115158
ISSN:
0378-8741
Subject:
Western blotting; X-radiation; apoptosis; blood serum; caspase-3; collagen; colon; dexamethasone; enteritis; epithelium; fibrosis; fluorescence; fluorescent antibody technique; heat; interleukin-6; irradiation; males; mitochondria; models; peroxidation; pro-apoptotic proteins; protein content; traditional medicine
Abstract:
... As a typical heat-clearing prescription, Shaoyao decoction (SYD) has a robust function of clearing viscera heat for the treatment of several intestinal discomfort symptoms. Clinical evidence indicated that it had the potential to cure radiation enteritis. However, its underlying mechanisms remain unclear. The present study was designed to probe the protective effects and the involved mechanisms of ...
DOI:
10.1016/j.jep.2022.115158

62. Anti-inflammatory and osteoprotective effects of Shi-Wei-Ru-Xiang pills on collagen-induced arthritis in rats via inhibiting MAPK and STAT3 pathways

Author:
Hui Xiong; Fengping Meng; Miao Luo; Weiwu Chen; Juan Tian; Lunju Chen; Yankun Ju; Zhinan Mei
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115693
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Western blotting; animal models; apoptosis; blood serum; cartilage; chondrocytes; coculture; flow cytometry; inflammation; interleukin-6; phosphorylation; protein synthesis; rheumatoid arthritis
Abstract:
... Shi-Wei-Ru-Xiang pills (SW) as a tradition Tibetan medicine has been clinically proved effective in rheumatoid arthritis (RA) treatment. However, the underlying mechanism of SW remains unclear. This study aimed to investigate the anti-arthritic effect of SW and its possible mechanisms of action. A CIA rat model in vivo, and IL-1β-stimulated synoviocytes or chondrocytes and a co-culture system (IL- ...
DOI:
10.1016/j.jep.2022.115693

63. Anti-inflammatory effects of Eclipta prostrata Linné on house dust mite-induced atopic dermatitis in vivo and in vitro

Author:
Yun-Mi Kang; Hye-Min Kim; Hwan Lee; Dong-Sung Lee; Hyo-Jin An
Source:
Journal of ethnopharmacology 2022 v.292 pp. 115233
ISSN:
0378-8741
Subject:
CD4-positive T-lymphocytes; Eclipta prostrata; Western blotting; anti-inflammatory activity; atopic dermatitis; dermis; diabetes; dust; dust mites; enzyme-linked immunosorbent assay; histology; immune response; inflammation; jaundice; keratinocytes; medicinal plants; mitogen-activated protein kinase; obesity; people; phosphorylation; polymerase chain reaction; signal transduction; therapeutics; traditional medicine; transactivators
Abstract:
... Atopic dermatitis (AD) is a kind of inflammation on the skin following with swollen, itchy, dryness and cracked skin. Though the exact cause of AD is unknown, there are evidence that people with AD have a compromised skin barrier along with inflammation. Eclipta prostrata Linné is a traditional herbal medicinal plant, has been used for the diabetes, obesity, jaundice, and inflammation. We supposed ...
DOI:
10.1016/j.jep.2022.115233

64. Anti-inflammatory effects of aucubin in cellular and animal models of rheumatoid arthritis

Author:
Yan ZHANG; Li-Dong TANG; Jian-Ying WANG; Hao WANG; Xiao-Yun CHEN; Lei ZHANG; Ying YUAN
Source:
Chinese journal of natural medicines 2022 v.20 no.6 pp. 458-472
ISSN:
1875-5364
Subject:
Western blotting; animal models; anti-inflammatory activity; apoptosis; aucubin; bone metabolism; cell differentiation; cell movement; fluorescent antibody technique; gene expression; histology; humans; inflammation; luciferase; micro-computed tomography; osteoclasts; pharmacodynamics; protein synthesis; reporter genes; rheumatoid arthritis
Abstract:
... Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. It is known that aucubin (AU) exerts anti-inflammatory activity, but its effects and mechanisms in RA are unclear. This study investigated the anti-inflammatory effects and mechanisms of AU in vivo and in vitro. Human fibroblast-like synoviocyte cells from patients with RA (HFLS-RA), RAW264.7 cells, and MC3T3-E1 cells were use ...
DOI:
10.1016/S1875-5364(22)60182-1

65. Anti-inflammatory effects of flavonoids and phenylethanoid glycosides from Hosta plantaginea flowers in LPS-stimulated RAW 264.7 macrophages through inhibition of the NF-κB signaling pathway

Author:
Li Yang; Junwei He
Source:
BMC complementary medicine and therapies 2022 v.22 no.1 pp. 55
ISSN:
2662-7671
Subject:
Hosta plantaginea; Western blotting; alternative medicine; enzyme-linked immunosorbent assay; flavonoids; flowers; glycosides; interleukin-6; lipopolysaccharides; macrophages; medicine; nitric oxide; prostaglandins; protein content; protein synthesis; secretion; toxicity; transcription factor NF-kappa B; viability; China
Abstract:
... BACKGROUND: The flower of Hosta plantaginea (Lam.) Aschers has traditionally been used in China as an important Mongolian medicine for the treatment of inflammatory diseases with limited scientific evidence. In previous studies, 16 flavonoids and 3 phenylethanoid glycosides (1–19) were isolated from the ethanolic extract of H. plantaginea flowers. Nevertheless, the anti-inflammatory effects of the ...
DOI:
10.1186/s12906-022-03540-1

66. Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Author:
Weiyu Fu; Xuehua Liao; Qian Zhang; Yuzhen Zhu; Si Mei; Qian Li; Xin Zhou; Xiaojun Li; Hui Luo; Hua Ye; Kefeng Wu
Source:
BMC complementary medicine and therapies 2022 v.22 no.1 pp. 174
ISSN:
2662-7671
Subject:
Clausena lansium; Fourier transform infrared spectroscopy; Western blotting; alternative medicine; antioxidant activity; arabinose; enzymes; fruits; galactose; galacturonic acid; gel chromatography; glucose; glucuronic acid; humans; mannose; melanin; melanogenesis; melanoma; models; molecular weight; mushrooms; nuclear magnetic resonance spectroscopy; pectins; rhamnose; ribose; tyrosine; water solubility
Abstract:
... BACKGROUND: Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical ...
DOI:
10.1186/s12906-022-03646-6

67. Anti-neuroinflammatory activity of 6,7-dihydroxy-2,4-dimethoxy phenanthrene isolated from Dioscorea batatas Decne partly through suppressing the p38 MAPK/NF-κB pathway in BV2 microglial cells

Author:
Ji Sun Lim; Jisun Oh; Hyun Seok Yun; Jeong Soon Lee; Dongyup Hahn; Jong-Sang Kim
Source:
Journal of ethnopharmacology 2022 v.282 pp. 114633
ISSN:
0378-8741
Subject:
Dioscorea polystachya; Japan; Western blotting; cell viability; coculture; cytotoxicity; enzyme-linked immunosorbent assay; heme oxygenase (biliverdin-producing); inducible nitric oxide synthase; interleukin-6; lipopolysaccharides; mice; mitogen-activated protein kinase; necrosis; neoplasms; neurodegenerative diseases; neuroglia; neurons; nitric oxide; phenanthrenes; phosphorylation; prostaglandin synthase; rhizomes; therapeutics; traditional medicine; transcription (genetics); yams; China; Korean Peninsula; Taiwan
Abstract:
... The rhizome of Dioscorea batatas Decne (called Chinses yam) widely distributed in East Asian countries including China, Japan, Korea and Taiwan has long been used in oriental folk medicine owing to its tonic, antitussive, expectorant and anti-ulcerative effects. It has been reported to have anti-inflammatory, antioxidative, cholesterol-lowering, anticholinesterase, growth hormone-releasing, antifu ...
DOI:
10.1016/j.jep.2021.114633

68. Anti-tumor effect of Yanggan Huayu granule by inducing AKT-mediated apoptosis in hepatocellular carcinoma

Author:
Yang Shen; Fan Yang; Haiyan Peng; Guangji Zhang; Fangfang Zhu; Haojun Xu; Le Shi
Source:
Journal of ethnopharmacology 2022 v.282 pp. 114601
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Western blotting; antineoplastic activity; apoptosis; blood serum; cell growth; flow cytometry; hepatoma; mice; models; protein synthesis; xenotransplantation
Abstract:
... Yanggan Huayu granule (YGHY) is a formula of traditional Chinese medicine that has been widely used to treat patients with liver cancer. But its working mechanism is still poorly understood.Aim of the study: To investigate the anti-tumor effect of YGHY and its working mechanisms in hepatocellular carcinoma (HCC).H22 mouse xenograft model was used to detect the effect of YGHY on hepatocellular carc ...
DOI:
10.1016/j.jep.2021.114601

69. Anti-viral activity of methyl 1-chloro-7-methyl-2-propyl-1h-benzo[d] imidazole-5-carboxylate against white spot syndrome virus in freshwater crab (Paratelphusa hydrodromous)

Author:
K. Karthikeyan; A. T. Manish Kumar; D. Harshini; G. Pooja; J. Arul Daniel; Pavani Yasam; G. Gracevictoria; M. Priayanka; P. Krishnaraj; R. Vidya; S. Asha Devi; Sabbasani Rajasekhara Reddy; Raja Sudhakaran
Source:
Aquaculture international 2022 v.30 no.2 pp. 989-998
ISSN:
0967-6120
Subject:
Penaeidae; Western blotting; White spot syndrome virus; antiviral properties; crabs; financial economics; freshwater; histopathology; markets; polymerase chain reaction; survival rate; tissues; white spot syndrome
Abstract:
... Shrimp farming industries are subjected to severe economic loss due to a disease called white spot syndrome, a viral disease caused by white spot syndrome virus (WSSV) in penaeid shrimp. Numerous active compounds in the market possess anti-viral activity against the white spot syndrome virus, yet the issue remains unsolved. The present study was carried out to determine the anti-viral activity of ...
DOI:
10.1007/s10499-021-00714-5

70. Antiangiogenic and apoptotic effects of benzyl caffeate on human umbilical vein endothelial cells (HUVECs) and chick embryo chorioallantoic membrane (CAM): In vitro and in vivo models

Author:
Ho-Jeong Bang; Eunju Yoon; Sun Wook Kim; Mok-Ryeon Ahn
Source:
Journal of functional foods 2022 v.93 pp. 105079
ISSN:
1756-4646
Subject:
Western blotting; angiogenesis; antioxidant activity; apoptosis; caffeic acid; caspase-3; caspase-9; chick embryos; chorioallantoic membrane; propolis
Abstract:
... Caffeic acid and its derivatives are polyphenolic compounds, which are present in high concentration in propolis and medicinal plants. Their antioxidant activity has been reported; however, the antiangiogenic effects of benzyl caffeate remain unclear. In this study, we investigated the angiogenic and apoptotic effects of benzyl caffeate both in vitro on HUVECs and in vivo in chick embryo chorionic ...
DOI:
10.1016/j.jff.2022.105079

71. Anticancer effects of imidazole nucleus in hepatocellular carcinoma cell lines via the inhibition of AKT and ERK1/2 signaling pathways

Author:
Erkan Kahraman; Erdem Göker
Source:
Molecular biology reports 2022 v.49 no.6 pp. 4377-4388
ISSN:
0301-4851
Subject:
Western blotting; antineoplastic activity; apoptosis; autophagy; cancer therapy; caspase-3; cell culture; cell movement; cell structures; cell viability; drugs; hepatoma; imidazole; molecular biology; neoplasm cells; viability assays
Abstract:
... BACKGROUND: Imidazole nucleus has been used efficiently in the development of many drug molecules due to its therapeutic effects. Many derivatives of it have been produced particularly for use in cancer treatment. However, the anti-cancer effects of imidazole nucleus in liver cancer cells are as yet unclear. In this study, we aimed to investigate the anti-cancer effects of imidazole nucleus in hep ...
DOI:
10.1007/s11033-022-07273-9

72. The Antioxidant Role of Selenium via GPx1 and GPx4 in LPS-Induced Oxidative Stress in Bovine Endometrial Cells

Author:
Samson O. Adeniran; Peng Zheng; Rui Feng; Elikanah O. Adegoke; Fushuo Huang; Mingjun Ma; Ziming Wang; Olamigoke O. Ifarajimi; Xiaoyu Li; Guixue Zhang
Source:
Biological trace element research 2022 v.200 no.3 pp. 1140-1155
ISSN:
0163-4984
Subject:
Western blotting; antioxidants; blood serum; cattle; cell culture; endometritis; endometrium; glutathione peroxidase; inflammation; lipopolysaccharides; oxidative stress; protective effect; research; selenium; selenomethionine; starvation; transcription factors
Abstract:
... This study investigated the antioxidant role of selenium (Se) in the form of selenomethionine (SLM) in LPS-induced oxidative stress via the glutathione peroxidase (GPx) enzymes and the Nrf2/HO-1 transcription factor. The impact of serum supplementation in culture media on GPxs was also studied. The bovine uterus is constantly exposed to exogenous pathogens postpartum, and the endometrium is the fi ...
DOI:
10.1007/s12011-021-02731-0

73. Antioxidant effect of Lactobacillus fermentum HFY02-fermented soy milk on D-galactose-induced aging mouse model

Author:
Tiantian Hu; Rui Chen; Yu Qian; Ke Ye; Xingyao Long; Kun-Young Park; Xin Zhao
Source:
Food science and human wellness 2022 v.11 no.5 pp. 1362-1372
ISSN:
2213-4530
Subject:
Lactobacillus fermentum; Western blotting; antioxidant activity; antioxidants; ascorbic acid; blood serum; brain; collagen; fermentation; food science; galactose; gene expression; genes; humans; inflammation; liver; mice; oxidation; peroxidation; probiotics; protein synthesis; research and development; soymilk; temperature
Abstract:
... This study aimed to investigate the antioxidant effect of soybean milk fermented by a new type of Lactobacillus fermentum (LF-HFY02) by using D-galactose induced aging mice model. Firstly, the optimal fermentation conditions was screened out by detecting the effects of different fermentation temperature and time on the active components and antioxidant activity of soybean milk in vitro. And then u ...
DOI:
10.1016/j.fshw.2022.04.036

74. Antioxidant mechanism of a newly found phenolic compound from adlay (NDPS) in HepG2 cells via Nrf2 signalling

Author:
Wenfei Xiong; Ya Li; Yijun Yao; Qian Xu; Lifeng Wang
Source:
Food chemistry 2022 v.378 pp. 132034
ISSN:
0308-8146
Subject:
Coix lacryma-jobi; Western blotting; antioxidant activity; cysteine synthase; food chemistry; genes; glutathione peroxidase; heme oxygenase (biliverdin-producing); oxidative stress; phytochemicals; protein content; superoxide dismutase; toxicity testing
Abstract:
... An in-depth understanding of the bioactive mechanism of phytochemicals has a good guiding value for the design of related functional foods. Herein, the effect of N¹, N⁵- di-[(E)-p-coumaroyl]-spermidine (NDPS) originated from adlay on protecting HepG2 cells from oxidative stress was evaluated by MTT assay, western blot and qRT-PCR. After pre-treatment of NDPS, the activities of antioxidant enzymes ...
DOI:
10.1016/j.foodchem.2021.132034

75. Antiproliferative Amaryllidaceae alkaloids from the bulbs of Hymenocallis littoralis (Jacq.) Salisb

Author:
Wenjing Ma; Siyu Wang; Yihai Wang; Jia Zeng; Jingwen Xu; Xiangjiu He
Source:
Phytochemistry 2022 v.197 pp. 113112
ISSN:
0031-9422
Subject:
Hymenocallis littoralis; Western blotting; alkaloids; apoptosis; cell movement; cytotoxicity; diastereomers; dose response; flow cytometry; human cell lines; humans; neoplasm cells; plant biochemistry; toxicity testing
Abstract:
... Seven undescribed Amaryllidaceae alkaloids classified into four types, including the plicamine-type, secoplicamine-type, belladine-type and pretazettine-type, along with another three alkaloids that have not been isolated from plant material and seven known alkaloids, were isolated from the bulbs of Hymenocallis littoralis (Jacq.) Salisb. The structures were elucidated on the basis of various spec ...
DOI:
10.1016/j.phytochem.2022.113112

76. Anti‐ulcerative effects of wogonin on ulcerative colitis induced by dextran sulfate sodium via Nrf2/TLR4/NF‐κB signaling pathway in BALB/c mice

Author:
Yadong Zhou; Fafu Dou; Huwei Song; Tao Liu
Source:
Environmental toxicology 2022 v.37 no.4 pp. 954-963
ISSN:
1520-4081
Subject:
Western blotting; apoptosis; body weight; caspase-3; colon; cytokines; dextran sulfate; ecotoxicology; flavonoids; histology; histopathology; inflammation; neutrophils; oxidative stress; rectum; sulfasalazine; therapeutics; ulcerative colitis
Abstract:
... Ulcerative colitis (UC) is an inflammatory disease on the deepest lining of the colon and rectum. Wogonin is an antitumor flavonoid, which possesses various therapeutic properties. Even if the anti‐colitis effect of wogonin was documented earlier, but the wogonin effect on inflammation underlying mechanism is not fully elucidated. In this present study, we hypothesized to study the oxidative damag ...
DOI:
10.1002/tox.23457

77. Apocynum venetum leaf extract alleviated doxorubicin-induced cardiotoxicity through the AKT/Bcl-2 signaling pathway

Author:
Yang Zhang; Shan Liu; Jiu-Long Ma; Chen Chen; Peng Huang; Jia-Hua Ji; Di Wu; Li-Qun Ren
Source:
Phytomedicine 2022 v.94 pp. 153815
ISSN:
0944-7113
Subject:
Apocynum venetum; Western blotting; adenosine; apoptosis; blood pressure; cardiomyocytes; cardiotoxicity; caspases; cyclophilins; diuresis; doxorubicin; flow cytometry; gene expression; kaempferol; lactate dehydrogenase; leaf extracts; membrane permeability; mitochondria; mitochondrial membrane; myricetin; oxidative stress; quercetin; rutin; sedation; toxicity testing
Abstract:
... Doxorubicin (DOX) is a broad-spectrum anti-tumor drug that has been associated with cardiotoxicity. Plant extracts have been shown to confer protection against DOX-induced cardiotoxicity. Apocynum venetum L. belongs to the Apocynaceae family. Flavonoid extracted from Apocynum venetum L. possess various biological effects, such as lowering blood pressure levels, sedation, diuresis, anti-aging, and ...
DOI:
10.1016/j.phymed.2021.153815

78. Apoptosis induction in human lung and colon cancer cells via impeding VEGF signaling pathways

Author:
Maryam Farzaneh Behelgardi; Zahra Gholami Shahvir; S. Mohsen Asghari
Source:
Molecular biology reports 2022 v.49 no.5 pp. 3637-3647
ISSN:
0301-4851
Subject:
B-cell lymphoma; Western blotting; angiogenesis; antagonists; apoptosis; cancer therapy; colon; colorectal neoplasms; flow cytometry; fluorescence microscopy; humans; lungs; mitogens; molecular biology; neoplasm cells; peptides; toxicity testing; vascular endothelial growth factors
Abstract:
... There is ample evidence to suggest that vascular endothelial growth factor (VEGF) is a potent mitogen factor in vasculogenesis and angiogenesis and that blockade of VEGF-mediated signals can also prevent tumor growth via enforcing cell apoptosis. In the current study, we assessed the suppressing effect of VGB4, a VEGF antagonist peptide with the binding ability to both VEGF receptor1 and VEGF rece ...
DOI:
10.1007/s11033-022-07203-9

79. Arborinine suppresses ovarian cancer development through inhibition of LSD1

Author:
Nan Li; Liang Yang; Hongling Zuo
Source:
Life sciences 2022 v.291 pp. 120275
ISSN:
0024-3205
Subject:
Western blotting; antineoplastic activity; antineoplastic agents; cancer therapy; carcinogenesis; cell lines; cell movement; cell proliferation; cytotoxicity; dose response; drug therapy; epithelium; females; gene expression; mice; neoplasm cells; ovarian neoplasms; paclitaxel; toxicity testing
Abstract:
... Epithelial ovarian carcinoma is the most lethal female reproductive malignancy in the world. Paclitaxel and carboplatin are generally the first-line treatment drugs for ovarian cancer patients, but numerous patients may develop chemotherapy resistance. Thus, it is urgent to identify novel drugs for ovarian cancer treatment. Arborinine has been known as a broad-spectrum anti-tumor agent due to it p ...
DOI:
10.1016/j.lfs.2021.120275

80. Arsenic trioxide increases apoptosis of SK-N-BE (2) cells partially by inducing GPX4-mediated ferroptosis

Author:
Chuchu Feng; Yu Wu; Yantao Chen; Xilin Xiong; Peng Li; Xiaomin Peng; Chunmou Li; Wenjun Weng; Yafeng Zhu; Dunhua Zhou; Yang Li
Source:
Molecular biology reports 2022 v.49 no.7 pp. 6573-6580
ISSN:
0301-4851
Subject:
Western blotting; apoptosis; arsenic oxide; central nervous system; cytotoxicity; death; ferroptosis; glutamate-ammonia ligase; glutathione; iron absorption; molecular biology; neoplasms; proteomics; therapeutics
Abstract:
... BACKGROUND: Neuroblastoma (NB) is the most common extracranial tumor in central nervous system threatening children’s health with limited therapeutic options. Arsenic trioxide (ATO) has been identified the cytotoxicity in NB cells but the potential mechanism remains unclear. In this study, we attempted to obtain some insight into the mechanisms of cell death induced by ATO in NB cells. METHODS AND ...
DOI:
10.1007/s11033-022-07497-9

81. Artemisia annua water extract attenuates DNCB-induced atopic dermatitis by restraining Th2 cell mediated inflammatory responses in BALB/c mice

Author:
Xinyan Han; Ziyu Chen; Jinfeng Yuan; Gaorui Wang; Xiao Han; Hui Wu; Hailian Shi; Guixin Chou; Liu Yang; Xiaojun Wu
Source:
Journal of ethnopharmacology 2022 v.291 pp. 115160
ISSN:
0378-8741
Subject:
Artemisia annua; Oriental traditional medicine; Western blotting; atopic dermatitis; blood serum; dermatomycoses; dexamethasone; ears; eczema; edema; enzyme-linked immunosorbent assay; eosin; gene expression; immunosuppression; inflammation; interleukin-13; interleukin-17; interleukin-4; interleukin-6; jaundice; malaria; mice; mitogen-activated protein kinase; phosphorylation; polymerase chain reaction; spleen; splenocytes; toluidine blue
Abstract:
... Artemisia annua L. (A. annua) is a traditional Chinese medicine that has been used since ancient times to treat malaria, eczema, dermatomycosis, jaundice, and boils. Modern pharmacological studies show that it has immunosuppressive and anti-inflammatory effects. However, the mechanism of A. annua in the treatment of atopic dermatitis (AD) remains unclear. This study was aimed to investigate the ef ...
DOI:
10.1016/j.jep.2022.115160

82. Ascorbic acid inhibits transcriptional activities of LXRα to ameliorate lipid metabolism disorder

Author:
Dandan Wang; Zequn Yin; Lan Han; Mengxue Zhang; Huaxin Li; Xiaoxiao Yang; Yuanli Chen; Shuang Zhang; Jihong Han; Yajun Duan
Source:
Journal of functional foods 2022 v.88 pp. 104901
ISSN:
1756-4646
Subject:
AMP-activated protein kinase; Western blotting; ascorbic acid; blood serum; dietary supplements; fatty acids; fatty liver; hepatocytes; hypertriglyceridemia; liver; transcription (genetics); triacylglycerols
Abstract:
... It has been reported that ascorbic acid inhibits non-alcoholic fatty liver disease partially by activating fatty acid β-oxidation. However, little is known about the mechanism of ascorbic acid-mediated lipid synthesis. Western blot, qRT-PCR and immunofluorescent staining were used to determine if ascorbic acid can regulate expression of liver X receptor α (LXRα) and AMP-activated protein kinase α ...
DOI:
10.1016/j.jff.2021.104901

83. Asperosaponin VI protects mice from sepsis by regulating Hippo and Rho signaling pathway

Author:
Caixia Pang; Cailing Wen; Yanxiang Liang; Hui Luo; Linlin Wei; Haiqian Liu; Tian Qin; Huijing Tan; Chonghua He; Ying Liu; Yang Chen; Siyu Zeng; Chun Zhou
Source:
Phytomedicine 2022 v.99 pp. 154010
ISSN:
0944-7113
Subject:
Western blotting; blood serum; bone marrow; histopathology; immunohistochemistry; inflammation; liver; lungs; protective effect; quantitative polymerase chain reaction; spleen; surgery; temperature; viability
Abstract:
... To explore the novel protective effect of Asperosaponin VI (AVI) on sepsis and its potential mechanism. In in vitro experiments, bone marrow mononuclear cells and THP-1-derived cells were used to evaluate the viability of AVI treatment. Besides, the quantitative real-time PCR and Western blot were adopted to explore the protective effect of AVI on LPS-induced inflammation. For in vivo work, the ef ...
DOI:
10.1016/j.phymed.2022.154010

84. Aspirin inhibits stem cell proliferation during freshwater Dugesia japonica regeneration by STAT3/SOX2/OCT4 signaling pathway

Author:
Ang Liang; Fan Wu; Chaojie Li; Yiyang Yu; Zimei Dong; Guangwen Chen; Fei Yu; Yanqing Yuwen; Dezeng Liu
Source:
Aquatic toxicology 2022 v.247 pp. 106158
ISSN:
0166-445X
Subject:
Dugesia; Western blotting; apoptosis; aspirin; cell proliferation; fluorescent antibody technique; freshwater; metabolites; municipal wastewater; protein synthesis; river water; salicylic acid; stem cells; therapeutics; toxicity; toxicology
Abstract:
... As a widely used drug in clinical practice, aspirin has a large number of residual drugs and metabolites discharged into the environment during the pharmaceutical process or after taking the drug. Aspirin content and its metabolite, salicylic acid, have been reported and detected in several river water samples and municipal wastewaters. However, little is known about the toxicity mechanisms of thi ...
DOI:
10.1016/j.aquatox.2022.106158

85. Assessment of BMP responses in an in vitro model of acute ethanol toxicity

Author:
Naila Habeeb; Sheyda Najafi; Jeanette C. Perron
Source:
MethodsX 2022 v.9 pp. 101631
ISSN:
2215-0161
Subject:
Western blotting; ethanol; models; phosphorylation; toxicity
Abstract:
... The assay presented here was designed to assess the immediate effects of ethanol (EtOH) exposure on intracellular signaling activated by BMPs (Bone Morphogenetic Proteins). Previous reports of the relationship between EtOH exposure and BMP-dependent signaling have primarily assessed the expression of individual BMPs, changes in BMP target genes or effects on the phosphorylation level of key downst ...
DOI:
10.1016/j.mex.2022.101631

86. Astaxanthin Alleviates Nonalcoholic Fatty Liver Disease by Regulating the Intestinal Flora and Targeting the AMPK/Nrf2 Signal Axis

Author:
Yuhang Li; Juxiong Liu; Bojian Ye; Yueyao Cui; Ruiqi Geng; Shu Liu; Yufei Zhang; Wenjin Guo; Shoupeng Fu
Source:
Journal of agricultural and food chemistry 2022 v.70 no.34 pp. 10620-10634
ISSN:
1520-5118
Subject:
AMP-activated protein kinase; Western blotting; acetyl-CoA carboxylase; astaxanthin; fatty liver; fatty-acid synthase; food chemistry; high fat diet; intestinal microorganisms; liver; oleic acid; oxidative stress; palmitic acid; protein synthesis; sterols
Abstract:
... Nonalcoholic fatty liver disease (NAFLD) is among the most prevalent chronic liver diseases around the globe. The accumulation of lipids in the liver and oxidative stress are important pathological mechanisms of NAFLD. Astaxanthin (AT) is a carotenoid extracted from shrimps and crabs with beneficial biological activities, including anti-oxidative and anti-inflammatory activities. 16S microflora se ...
DOI:
10.1021/acs.jafc.2c04476

87. Aulosirazoles B and C from the Cyanobacterium Nostoc sp. UIC 10771: Analogues of an Isothiazolonaphthoquinone Scaffold that Activate Nuclear Transcription Factor FOXO3a in Ovarian Cancer Cells

Author:
Lydia J. Davis; Amanda C. Maldonado; Manead Khin; Aleksej Krunic; Joanna E. Burdette; Jimmy Orjala
Source:
Journal of natural products 2022 v.85 no.3 pp. 540-546
ISSN:
1520-6025
Subject:
Nostoc; Western blotting; confocal microscopy; culture media; cytotoxins; fluorescent antibody technique; metabolites; ovarian neoplasms; transcription factors
Abstract:
... The known solid-tumor-selective cytotoxin aulosirazole (1) was identified from bioactive extracts from the culture medium of the cyanobacterium Nostoc sp. UIC 10771. Here, we demonstrate that 1 induces the nuclear accumulation of FOXO3a in OVCAR3 using both Western blot analysis and immunofluorescence confocal microscopy. We also report the discovery of two additional analogues, aulosirazoles B (2 ...
DOI:
10.1021/acs.jnatprod.1c01030

88. Autophagy activation by Terminalia chebula Retz. reduce Aβ generation by shifting APP processing toward non-amyloidogenic pathway in APPswe transgenic SH-SY5Y cells

Author:
Longhe Zhao; Zhaorong Yue; Yanni Wang; Jiatao Wang; Inam Ullah; Fahim Muhammad; Yongtao Zhou; Hongmei Zhu; Xin Wang; Hongyu Li
Source:
Phytomedicine 2022 v.103 pp. 154245
ISSN:
0944-7113
Subject:
Alzheimer disease; Terminalia chebula; Western blotting; acridine orange; amyloid; autophagy; cell viability; genetically modified organisms; metabolism; neurodegenerative diseases
Abstract:
... Alzheimer's disease (AD) is the most common neurodegenerative disease. Deposition of amyloid β plaques (Aβ) is a central hallmark of AD. Accumulating evidence suggest that shifting amyloid precursor protein (APP) metabolism pathway to non-amyloidogenic ways and inducing autophagy play key roles in AD pathology. In published reports, there is no research on the APP metabolic process of Terminalia c ...
DOI:
10.1016/j.phymed.2022.154245

89. Autophagy inhibitors alleviate Japanese encephalitis virus-induced cerebral inflammation in mice

Author:
Jinhua Zhang; Wei Han; Changqing Xie; Mingxing Gao; Xugang Wang; Xueying Hu; Wanpo Zhang; Shengbo Cao; Xiaoli Liu; Guofu Cheng; Changqin Gu
Source:
Archives of virology 2022 v.167 no.3 pp. 849-859
ISSN:
0304-8608
Subject:
Japanese encephalitis virus; Western blotting; autophagy; brain; encephalitis; heart diseases; immunohistochemistry; inflammation; mice; mitochondria; quantitative polymerase chain reaction; survival rate; transmission electron microscopy; virology; viruses
Abstract:
... Japanese encephalitis (JE) is a zoonotic epidemic disease caused by Japanese encephalitis virus (JEV), and currently, no medicines are available to treat this disease. Autophagy modulators play an important role in the treatment of tumors, heart disease, and some viral diseases. The aim of this study was to investigate the effects of autophagy modulators on JEV infection and the host response in m ...
DOI:
10.1007/s00705-021-05283-9

90. Autophagy modulation in rainbow trout Oncorhynchus mykiss L. and resistance to experimental infection with Flavobacterium psychrophilum

Author:
Juan‐Ting Liu; Phuc H. Pham; John S. Lumsden
Source:
Journal of fish diseases 2022 v.45 no.4 pp. 535-545
ISSN:
0140-7775
Subject:
Flavobacterium psychrophilum; Oncorhynchus mykiss; Western blotting; autophagy; chloroquine; deoxynivalenol; fish; fish diseases; liver; mortality; muscles; quantitative polymerase chain reaction; spleen
Abstract:
... Previously, rainbow trout fed deoxynivalenol (DON) or partially fed (pair‐fed) for 4 weeks before and during experimental infection with Flavobacterium psychrophilum had significantly decreased mortality rates. Similar results were obtained in the present study after 12 days, but not after 6 days, feeding 5 ppm DON or pair‐fed before infection. Furthermore, feeding 250 ppm chloroquine (CQ) also re ...
DOI:
10.1111/jfd.13578

91. Autophagy of hepatic stellate cell induced by Clonorchis sinensis

Author:
Bao Zheng; Zhiyan Gao; Liumei Liang; Yunyu Lu; Yongting Kong; Wanting Chen; Keying Lin; Wanqi Chen; Jingying Mai; Yanwen Li; Changling Ma
Source:
Molecular biology reports 2022 v.49 no.3 pp. 1895-1902
ISSN:
0301-4851
Subject:
Clonorchis sinensis; Western blotting; adults; autophagosomes; autophagy; cell proliferation; chloroquine; collagen; humans; liver cirrhosis; molecular biology; parasites; pathogenesis; plasmids; risk factors; secretion; tetrazolium
Abstract:
... BACKGROUND: Clonorchis sinensis was a food-borne zoonotic parasite in the worldwide and also an important risk factor of hepatic fibrosis. Excretory/secretion products of C. sinensis (CsESPs) are involved in parasite-host interactions and contribute to the development of hepatic damage. The aim of the present study was to investigate whether CsESPs and CsTP (adult protein) could induce autophagy o ...
DOI:
10.1007/s11033-021-07001-9

92. Autophagy-Sirtuin1(SIRT1) Alleviated the Coronary Atherosclerosis (AS) in Mice through Regulating the Proliferation and Migration of Endothelial Progenitor Cells (EPCs) via wnt/β-catenin/GSK3β Signaling Pathway

Author:
Y. Li; W. Cui; B. Song; X. Ye; Z. Li; C. Lu
Source:
journal of nutrition, health & aging 2022 v.26 no.3 pp. 297-306
ISSN:
1279-7707
Subject:
Western blotting; animal models; aorta; autophagy; biomarkers; blood; bone marrow; coronary artery disease; diet; fluorescent antibody technique; immunohistochemistry; intravenous injection; nicotinamide; risk; tail
Abstract:
... BACKGROUND AND PURPOSE: SIRT1 was associated with AS risk and EPCs were reported to participate in the endothelial repair in Coronary Atherosclerosis (CAS). In this study, we explored the role of SIRT1 in AS mice and also its modulation in EPCs. METHODS AND MATERIALS: ApoE−/−mice were fed on high-fat and high-glucose diet to establish the AS animal model with the normally-raised C57BL/6 mice as a ...
DOI:
10.1007/s12603-022-1750-7

93. B7-H4 reduction induced by Toxoplasma gondii infection results in dysfunction of decidual dendritic cells by regulating the JAK2/STAT3 pathway

Author:
Xinyue Sun; Hongbing Xie; Haixia Zhang; Zhidan Li; Houbao Qi; Chunyan Yang; Xianbing Liu; Liqin Ren; Yuzhu Jiang; Xuemei Hu
Source:
Parasites & vectors 2022 v.15 no.1 pp. 157
ISSN:
1756-3305
Subject:
Toxoplasma gondii; Western blotting; abortion (animals); fetal death; flow cytometry; humans; immunosuppression; indoleamine 2,3-dioxygenase; interleukin-10; interleukin-12; models
Abstract:
... BACKGROUND: Primary infection of Toxoplasma gondii can cause serious abnormal pregnancy outcomes such as miscarriage and stillbirth. Inhibitory molecule B7-H4 is abundantly expressed in dendritic cells (DCs) and plays an important role in maintaining immune tolerance. However, the role of B7-H4 in decidual DCs (dDCs) in T. gondii-induced abnormal pregnancy outcomes is not clear. METHODS: We establ ...
DOI:
10.1186/s13071-022-05263-1

94. BMAL1 induces colorectal cancer metastasis by stimulating exosome secretion

Author:
Pengjuan Dong; Yaping Wang; Yutong Liu; Chunting Zhu; Jiaxin Lin; Ruizhe Qian; Luchun Hua; Chao Lu
Source:
Molecular biology reports 2022 v.49 no.1 pp. 373-384
ISSN:
0301-4851
Subject:
Western blotting; centrifugation; circadian clocks; circadian rhythm; colorectal neoplasms; exosomes; humans; luciferase; metastasis; molecular biology; secretion; transcription (genetics); transmission electron microscopy
Abstract:
... BACKGROUND: To adapt to daily changes in the external environment, organisms have developed circadian rhythm systems with a period of approximately 24 h. Many studies have reported that both circadian rhythms and exosomes play important roles in the development and metastasis of tumors. However, whether circadian clock genes can affect the progression of tumors by regulating exosomes remains uncle ...
DOI:
10.1007/s11033-021-06883-z

95. BTBD10 inhibits glioma tumorigenesis by downregulating cyclin D1 and p-Akt

Author:
Yu Liu; Sen Li; Ruoping Chen; Juxiang Chen; Bo Xiao; Yicheng Lu; Jiangang Liu
Source:
Open life sciences 2022 v.17 no.1 pp. 907-916
ISSN:
2391-5412
Subject:
Lentivirus; Western blotting; apoptosis; brain; carcinogenesis; cell growth; cell proliferation; cyclins; flow cytometry; glioblastoma; humans; reverse transcriptase polymerase chain reaction; tetrazolium
Abstract:
... The aim of this study was to investigate the role of BTBD10 in glioma tumorigenesis. The mRNA and protein levels of BTBD10 in 52 glioma tissues and eight normal brain tissues were determined using reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis, respectively. U251 human glioblastoma cells were infected with BTBD10-expressing or control lentiviruses. Cell growth w ...
DOI:
10.1515/biol-2022-0103

96. BaZiBuShen alleviates cognitive deficits and regulates Sirt6/NRF2/HO-1 and Sirt6/P53-PGC-1α-TERT signaling pathways in aging mice

Author:
Lin Li; Hao Zhang; Beibei Chen; Bingke Xia; Ruyuan Zhu; Yage Liu; Xuan Dai; Zimengwei Ye; Dandan Zhao; Fangfang Mo; Sihua Gao; Alexander N. Orekhov; Marc Prentki; Lili Wang; Shuzhen Guo; Dongwei Zhang
Source:
Journal of ethnopharmacology 2022 v.282 pp. 114653
ISSN:
0378-8741
Subject:
B-lymphocytes; Western blotting; apoptosis; caspase-3; cerebral cortex; cognition; galactose; health services; heme oxygenase (biliverdin-producing); homeostasis; humans; immunohistochemistry; mechanism of action; memory; mice; mitochondria; sirtuins; telomerase; telomeres; traditional medicine
Abstract:
... BaZiBuShen formula (BZBS) is clinically used to counteract mental fatigue and to retard the aging process. Brain aging echoes in major risks of human sufferings and has become one of the main challenges to our societies and the health-care systems.To investigate the effect and mode of action of BZBS on aging-associated cognitive impairments.BZBS was orally administered to D-galactose and NaNO₂-ind ...
DOI:
10.1016/j.jep.2021.114653

97. Bacillus subtilis ameliorates Escherichia coli-induced endometritis in mice via maintaining endometrial barrier and inhibiting inflammatory response

Author:
Shuang Li; Yiqi Wang; Lianjun Feng; Zhongxian Yu; Min Qiu; Ying Wang; Naisheng Zhang; Xiaoyu Hu; Yunhe Fu
Source:
Microbial pathogenesis 2022 v.166 pp. 105487
ISSN:
0882-4010
Subject:
Bacillus subtilis; Escherichia coli; Western blotting; animal fertility; animal husbandry; endometritis; endometrium; enzyme-linked immunosorbent assay; epithelium; immunostimulants; industry; inflammation; models; myeloperoxidase; neutrophils; occludins; parturition; pathogenesis; protective effect; quantitative polymerase chain reaction; tight junctions
Abstract:
... Endometritis is a common obstetric disease that occurs most frequently after parturition in a variety of animals. Animal infertility due to endometritis severely hinders animal husbandry and often causes serious economic losses to the dairy farming industry. According to reports, Bacillus subtilis (B. subtilis) can prevent pathogenic colonization of epithelial cells and exert immunostimulatory eff ...
DOI:
10.1016/j.micpath.2022.105487

98. Bai-Hu-Tang regulates endothelin-1 and its signalling pathway in vascular endothelial cells

Author:
Min Feng; Dongsheng Wang; Xurong Wang; Ying Yang; Shidong Zhang
Source:
Journal of ethnopharmacology 2022 v.284 pp. 114812
ISSN:
0378-8741
Subject:
Western blotting; blood serum; body weight; colorimetry; endothelin receptors; endothelins; fever; gene expression; humans; hydrophilicity; interleukin-6; intravenous injection; lipopolysaccharides; models; nitric oxide; nitric oxide synthase; nitrites; phosphatidylinositol 3-kinase; protein synthesis; quantitative polymerase chain reaction; rabbits; secretion; smooth muscle; sweat; thirst; toxicity testing; traditional medicine; vasodilation
Abstract:
... Bai-Hu-Tang (BHT) is traditionally used to treat human and animal fever syndrome with four symptoms: large and vigorous pulse, large thirst, high sweat, and high heat.To investigate the mechanism of vasodilation regulation of Bai-Hu-Tang in primary vascular endothelial cells stimulated by lipopolysaccharide (LPS).A hydrophilic concentrate of BHT was prepared, and the main components of mangiferin ...
DOI:
10.1016/j.jep.2021.114812

99. Bartonella henselae Recombinant Pap31 for the Diagnosis of Canine and Human Bartonelloses

Author:
Pradeep Neupane; Ricardo G. Maggi; Manoj Basnet; Erin Lashnits; Gerard P. Andrews; Edward B. Breitschwerdt
Source:
Pathogens 2022 v.11 no.2 pp. -
ISSN:
2076-0817
Subject:
Bartonella henselae; Western blotting; antibodies; bacterial adhesion; bacterial colonization; dogs; heme; humans; immunogenicity; serodiagnosis; surface proteins; zoonoses
Abstract:
... Bartonella spp. comprise a genus of Gram-negative alphaproteobacteria that are slow growing, fastidious, and facultative intracellular pathogens with zoonotic potential. Immunofluorescent antibody assays (IFAs), Western blot (WB), and enzyme-linked immunosorbent assays (ELISAs), the frequently used modalities for the serological diagnosis of canine and human Bartonelloses, generate numerous false ...
DOI:
10.3390/pathogens11020182

100. Bellidifolin from Gentianella acuta (Michx.) Hulten protects H9c2 cells from hydrogen peroxide-induced injury via the PI3K-Akt signal pathway

Author:
Siqi Li; Congying Huang; Xing Li; Xiangxi Meng; Rong Wen; Xiaodong Zhang; Chunhong Zhang; Minhui Li
Source:
Toxicology reports 2022 v.9 pp. 1655-1665
ISSN:
2214-7500
Subject:
Western blotting; alanine transaminase; antioxidant enzymes; apoptosis; cardiomyocytes; creatine kinase; death; dose response; enzyme activity; glutathione peroxidase; glycogen (starch) synthase; humans; hydrogen peroxide; lactate dehydrogenase; liquid chromatography; mass spectrometry; medicinal plants; medicine; models; morbidity; mortality; myocardial ischemia; oxidative stress; pharmacology; protective effect; rats; signal transduction; superoxide dismutase; therapeutics; toxicology; transcriptome; China
Abstract:
... Cardiovascular disease is the most common disease in the world and the first among the causes of human death. Its morbidity and mortality increase annually, but no effective treatment is available. Therefore, new drugs should be developed to treat cardiovascular disease. Gentianella acuta (Michx.) Hulten (G. acuta) is an important Mongolian medicine in China and elicits protective effects on cardi ...
DOI:
10.1016/j.toxrep.2022.08.006