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1. 23-O-acetylshengmanol-3-O-α-L-arabinoside alleviates lipopolysaccharide-induced acute lung injury through inhibiting IκB/NF-κB and MAPK/AP-1 signaling pathways

Author:
Chunyan Chen; Lin Li; Xiaomei Liu; Deqin Zhang; Yang Liu; Yuhong Li
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115725
ISSN:
0378-8741
Subject:
Actaea cimicifuga; Oriental traditional medicine; Western blotting; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; fluorescent antibody technique; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; phosphorylation; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
DOI:
10.1016/j.jep.2022.115725

2. Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221

Author:
Jie Yang; Zi-Xuan Wang; Lei Fang; Tu-Shuai Li; Zhi-Hong Liu; Ying Pan; Ling-Dong Kong
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115688
ISSN:
0378-8741
Subject:
Atractylodes lancea; Magnolia officinalis; Oriental traditional medicine; Western blotting; adiponectin receptors; agonists; animal models; diet; fluorescent antibody technique; fructose; humans; insulin; insulin resistance; kidneys; liquid chromatography; non-specific serine/threonine protein kinase; pharmacology; rats; tandem mass spectrometry; therapeutics; thermosensitivity
Abstract:
... In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu's functions including Kidney deficiency. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection. To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by hig ...
DOI:
10.1016/j.jep.2022.115688

3. Calcium pyruvate attenuates fat deposition by augmenting fatty acid oxidation and inhibiting glucose oxidation in juvenile large yellow croaker (Larimichthys crocea) consuming a high-fat diet

Author:
Yunqiang Zhang; Wencong Lai; Qiuchi Chen; Wei Fang; Kun Cui; Yuliang He; Xueshan Li; Kangsen Mai; Qinghui Ai
Source:
Aquaculture 2023 v.562 pp. 738778
ISSN:
0044-8486
Subject:
AMP-activated protein kinase; Larimichthys crocea; Western blotting; acetyl-CoA carboxylase; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; beta oxidation; blood glucose; calcium; carnitine palmitoyltransferase; catalase; citrates; diacylglycerol acyltransferase; dietary supplements; droplet size; fatty acid transport proteins; fatty-acid synthase; fish; gene expression; glucokinase; glucose; growth performance; hepatosomatic index; hexokinase; high density lipoprotein cholesterol; high fat diet; interleukin-1beta; juveniles; lipemic effect; lipogenesis; liver; low density lipoprotein cholesterol; malate dehydrogenase; muscles; obesity; oxidation; phosphofructokinases; prostaglandin synthase; protein synthesis; pyruvate dehydrogenase (lipoamide); pyruvate kinase; pyruvic acid; succinate dehydrogenase (quinone); triacylglycerol lipase; triacylglycerols; weight gain
Abstract:
... Calcium pyruvate (CP), known as an energetic aid, has been shown to enhance lipid utilization and prevent obesity in mammals. However, very few studies were conducted to evaluate the potential implications of CP for aquatic animals. This study examined the role of CP on fat deposition in large yellow croaker by feeding fish with high-fat diets containing four levels of dietary CP (0, 0.375%, 0.75% ...
DOI:
10.1016/j.aquaculture.2022.738778

4. Celastrus orbiculatus Thunb. extract inhibits EMT and metastasis of gastric cancer by regulating actin cytoskeleton remodeling

Author:
Haibo Wang; YuanYuan Luo; Shiya Ou; Tengyang Ni; Zewen Chu; Xinyi Feng; Xiaojun Dai; Xiaochun Zhang; Yanqing Liu
Source:
Journal of ethnopharmacology 2023 v.301 pp. 115737
ISSN:
0378-8741
Subject:
Celastrus orbiculatus; Oriental traditional medicine; Western blotting; actin; analgesics; animals; cell movement; medicinal plants; metalloproteinases; metastasis; microfilaments; neoplasm cells; protein degradation; regulatory proteins; stomach neoplasms; China
Abstract:
... The traditional Chinese medicine herb Celastrus orbiculatus Thunb. is an important folk medicinal plant in China that has been used as an anti-inflammatory, antitumor, and analgesic in various diseases. Recent years, many studies have reported the significant effects of Celastrus orbiculatus Thunb. extract (COE) on gastric cancer. However, the specific mechanism by which COE regulates gastric canc ...
DOI:
10.1016/j.jep.2022.115737

5. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
DNA; DNA damage; Sparus aurata; Western blotting; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

6. Uncovering the effect and mechanism of Panax notoginseng saponins on metabolic syndrome by network pharmacology strategy

Author:
Yisa Wang; Peng Ma; Zijing Wang; Mingxia Sun; Biyu Hou; Tianshu Xu; Wenlan Li; Xiuying Yang; Guanhua Du; Tengfei Ji; Guifen Qiang
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115680
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Panax notoginseng; Western blotting; adipocytes; adiposity; blood glucose; blood stasis; brown adipose tissue; computer simulation; drugs; energy; fatty liver; glucose; heat production; high fat diet; histopathology; insulin tolerance test; liver; metabolic diseases; metabolic syndrome; metabolism; obesity; pharmacology; protective effect; quantitative polymerase chain reaction; saponins; stomach; therapeutics; weight loss; white adipose tissue
Abstract:
... Metabolic syndrome (MetS) is a cluster of disease centered on obesity, which is the result of stagnation of liver qi according to traditional Chinese medicine. Panax notoginseng is a traditional Chinese herbal medicine, entering liver and stomach meridians and dissipating blood stasis, in which panax notoginseng saponins (PNS) are the main active components. However, its effects and mechanism on m ...
DOI:
10.1016/j.jep.2022.115680

7. Xianglian Pill attenuates ulcerative colitis through TLR4/MyD88/NF-κB signaling pathway

Author:
Yuxin Dai; Qiulu Lu; Peiyi Li; Junyu Zhu; Jiaxin Jiang; Tong Zhao; Yue Hu; Kang Ding; Min Zhao
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115690
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Western blotting; blood serum; colon; dextran sulfate; enzyme activity; enzyme-linked immunosorbent assay; fluorescent antibody technique; histopathology; interleukin-18; interleukin-6; liquid chromatography; models; myeloperoxidase; necrosis; neoplasms; protein domains; quantitative polymerase chain reaction; salicylic acid; ubiquitination; ulcerative colitis
Abstract:
... Xianglian Pill (XLP) is a classical Chinese medicine prescription applied for controlling ulcerative colitis (UC). Whereas, the underlying mechanism remains unclear. The present work was aimed to investigate the mechanism of XLP in dextran sulfate sodium (DSS)-induced UC via the Toll Like Receptor 4 (TLR4)/Myeloid Differentiation factor 88 (MyD88)/Nuclear Factor kappa-B (NF-κB) signaling in mice.  ...
DOI:
10.1016/j.jep.2022.115690

8. The crude extract from the flowers of Trollius chinensis Bunge exerts anti-influenza virus effects through modulation of the TLR3 signaling pathway

Author:
Yuxi Liang; Xiaoli Liu; Jingyan Hu; Songli Huang; Xin Ma; Xiaoyan Liu; Rufeng Wang; Xiuhua Hu
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115743
ISSN:
0378-8741
Subject:
Influenza A virus; Oriental traditional medicine; Trollius chinensis; Western blotting; blood; cytoplasm; epithelium; fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
DOI:
10.1016/j.jep.2022.115743

9. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

10. (±)-Equol interferes with the secretion of gonadotropin-releasing hormone and the expression of neuromodulators in murine neurons

Author:
Jingyuan Xiong; Minghui Wang; Ye Tian; Aru Ling; Shufang Shan; Li Zhao; Guo Cheng
Source:
Toxicological and environmental chemistry 2022 v.104 no.1 pp. 129-140
ISSN:
1029-0486
Subject:
RING finger domains; Western blotting; cell viability; daidzein; environmental science; enzyme-linked immunosorbent assay; estrogens; females; gonadotropin-releasing hormone; intestines; menopause; metabolites; mice; neurotransmitters; quantitative polymerase chain reaction; secretion; toxicology
Abstract:
... S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the ef ...
DOI:
10.1080/02772248.2022.2055031

11. (−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma

Author:
I-Ta Lu; Shih-Chao Lin; Yi-Chia Chu; Ya Wen; You-Cheng Lin; Wen-Chien Cheng; Jyh-Horng Sheu; Chi-Chien Lin
Source:
Marine drugs 2022 v.20 no.2 pp. -
ISSN:
1660-3397
Subject:
Western blotting; antibacterial properties; apoptosis; caspases; cell viability; death domain receptors; endoplasmic reticulum; guanidines; hepatoma; humans; liver; membrane potential; methanol; mitochondria; mitochondrial membrane; sesquiterpenoids
Abstract:
... Liver cancers, such as hepatocellular carcinoma (HCC), are a highly prevalent cause of cancer-related deaths. Current treatments to combat liver cancer are limited. (−)-Agelasidine A, a compound isolated from the methanol extract of Agelasnakamurai, a sesquiterpene guanidine derived from sea sponge, has antibacterial activity. We demonstrated its anticancer capabilities by researching the associat ...
DOI:
10.3390/md20020109

12. (−)-Epicatechin modulates the expression of myomiRs implicated in exercise response in mouse skeletal muscle

Author:
Carlos Palma-Flores; Paola B. Zárate-Segura; J. Manuel Hernández-Hernández; Sergio de los Santos; Andrea S. Tejeda-Gómez; Luis Javier Cano-Martínez; Patricia Canto; Jorge Omar Garcia-Rebollar; Ramón M. Coral-Vázquez
Source:
Gene 2023 v.849 pp. 146907
ISSN:
0378-1119
Subject:
Western blotting; bioinformatics; exercise; genes; hypertrophy; mice; microRNA; muscle development; muscles; myogenin; signal transduction; skeletal muscle
Abstract:
... The flavanol (−)-epicatechin has exercise-mimetic properties. Besides, several miRNAs play a role in modulating the adaptation of the muscle to different training protocols. However, notwithstanding all information, few studies aimed to determine if (−)-epicatechin can modify the expression of miRNAs related to skeletal muscle development and regeneration. Mice were treated for fifteen days by ora ...
DOI:
10.1016/j.gene.2022.146907

13. 17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner

Author:
Ana Laura Pereira-Suárez; María de la Luz Galván-Ramírez; Laura Rocío Rodríguez-Pérez; Edgar I. López-Pulido; Christian David Hernández-Silva; Inocencia Guadalupe Ramírez-López; Grecia Viridiana Morales Amaya; Luis David Lopez Cabrera; José Francisco Muñoz-Valle; Adrián Ramírez-de-Arellano
Source:
Molecular and biochemical parasitology 2022 v.247 pp. 111433
ISSN:
0166-6851
Subject:
Toxoplasma gondii; Western blotting; cell growth; estrogen receptors; inflammation; macrophages; models; monocytes; parasites; parasitology; phosphatidylinositol 3-kinase; pregnancy; prolactin receptors; tachyzoites
Abstract:
... Toxoplasma gondii (T. gondii) is a parasite common in pregnancy. Monocytes and macrophages are a significant immunologic barrier against T. gondii by boosting up inflammation. This outcome is highly regulated by signaling pathways such as MAPK (ERK1/2) and PI3K (AKT), necessary in cell growth and proliferation. It may be associated with the hormonal receptors’ modulation by T. gondii (Estrogen Rec ...
DOI:
10.1016/j.molbiopara.2021.111433

14. 17β-estradiol reduces NF-κB expression induced by increased crosstalk between KLF5 and ERα in murine vascular smooth muscle cells

Author:
Man-li Zhang; Man-na Zhang; Wen-li Wang; Hui Chen; Xia Wang; Xuan Li; Li Li; Fei Tong
Source:
Biochemical and biophysical research communications 2022 v.625 pp. 181-187
ISSN:
0006-291X
Subject:
Western blotting; cardiovascular system; cell proliferation; chromatin immunoprecipitation; fluorescent antibody technique; inflammation; luciferase; research; smooth muscle
Abstract:
... Pathological vascular remodeling and cell proliferation play vital roles in many proliferative vascular diseases. Estrogen can protect the cardiovascular system, but its exact molecular mechanism is unknown. Here we report that 17β-estradiol (E2) suppressed vascular smooth muscle cells (VSMCs) proliferation and inflammation. qRT-PCR and Western blot demonstrated that E2 decreased NF-κB p50 express ...
DOI:
10.1016/j.bbrc.2022.07.054

15. 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model

Author:
Mojtaba Malekinejad; Mohammad Reza Pashaee; Hassan Malekinejad
Source:
European journal of nutrition 2022 v.61 no.7 pp. 3437-3447
ISSN:
1436-6207
Subject:
Western blotting; absorption; adhesion; biochemical pathways; cell viability; cytotoxicity; dietary supplements; electrical resistance; epithelium; glycyrrhizin; human cell lines; humans; intestines; luciferase; metabolites; models; occludins; permeability; protein content; tight junctions; toxicity testing; traditional medicine
Abstract:
... PURPOSE: Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated. METHODS: The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity usin ...
DOI:
10.1007/s00394-022-02900-4

16. 2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis

Author:
Mohammed W. Al-Rabia; Mohamed A. Alfaleh; Hani Z. Asfour; Waleed S. Alharbi; Mohamed A. El-Moselhy; Nabil A. Alhakamy; Usama A. Fahmy; Osama A. A. Ahmed; Omar Fahmy; Omar M. Rashad; Abdulmohsin J. Alamoudi; Ashraf B. Abdel-Naim
Source:
Antioxidants 2022 v.11 no.8 pp. -
ISSN:
2076-3921
Subject:
Western blotting; antioxidants; bioavailability; cyclosporine; kidneys; males; micelles; nanocarriers; nephrotoxicity; oral administration; particle size; polyethylene glycol; protein synthesis; succinic acid
Abstract:
... The immunosuppressant cyclosporine A (CSA) has been linked to serious renal toxic effects. Although 2-methoxyestradiol (2ME) possesses a wide range of pharmacological abilities, it suffers poor bioavailability after oral administration. The purpose of this study was to evaluate the potential of 2ME loaded D-ɑ-tocopheryl polyethylene glycol succinate (TPGS) micelles to prevent CSA-induced nephrotox ...
DOI:
10.3390/antiox11081499

17. 20 (S)-ginsenoside Rh2 inhibits colorectal cancer cell growth by suppressing the Axl signaling pathway in vitro and in vivo

Author:
Haibo Zhang; Jun-Koo Yi; Hai Huang; Sijun Park; Wookbong Kwon; Eungyung Kim; Soyoung Jang; Si-Yong Kim; Seong-kyoon Choi; Duhak Yoon; Sung-Hyun Kim; Kangdong Liu; Zigang Dong; Zae Young Ryoo; Myoung Ok Kim
Source:
Journal of Ginseng Research 2022 v.46 no.3 pp. 396-407
ISSN:
1226-8453
Subject:
Panax; Western blotting; antineoplastic activity; apoptosis; cell cycle checkpoints; cell growth; cell proliferation; colorectal neoplasms; morbidity; mortality; neoplasm cells; research; toxicity; transfection; xenotransplantation
Abstract:
... Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenoside Rh2 (G-Rh2) is a natural compound extracted from ginseng, which exhibits anticancer effects in many cancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo. Cell proliferation, migration, invasion, apoptosis, cell cycle, and western ...
DOI:
10.1016/j.jgr.2021.07.004

18. 3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

Author:
Jiajun Liu; Wen Hu; Xiaoying Ma; Xin Liang; Long Lin; Jianming Huang; Jianwen Liu
Source:
Environmental toxicology 2022 v.37 no.2 pp. 349-361
ISSN:
1520-4081
Subject:
Azolla; Western blotting; agonists; apoptosis; caspases; cell lines; cytotoxicity; digestive system; ecotoxicology; epithelial cells; immune system; membrane potential; mitochondria; radioprotective effect; radiotherapy
Abstract:
... Ionizing radiation (IR) brings many health problems to humans, causing damage to the digestive system, hematopoietic system, and immune system. Natural compounds derived from plants have attracted widespread attention due to their low toxicity. Here, we found that 3,4,5‐O‐tricaffeoylquinic acid (tCQA) extracted from natural plant Azolla imbricata could significantly alleviate the systemic damage i ...
DOI:
10.1002/tox.23403

19. 3,4-Dihydroxyphenylacetic acid ameliorates gut barrier dysfunction via regulation of MAPK-MLCK pathway in type 2 diabetes mice

Author:
Min Liu; Li Wang; Bijun Huang; Qun Lu; Rui Liu
Source:
Life sciences 2022 v.305 pp. 120742
ISSN:
0024-3205
Subject:
Western blotting; absorption barrier; anti-inflammatory activity; blood glucose; body weight; digestive system diseases; glutathione; high fat diet; hyperglycemia; inflammation; insulin; insulin resistance; interleukin-10; intestines; lipopolysaccharides; malondialdehyde; mitogen-activated protein kinase; myosin; noninsulin-dependent diabetes mellitus; oxidative stress; protein synthesis; streptozotocin; superoxide dismutase; tight junctions
Abstract:
... This study aims to investigate whether 3,4-dihydroxyphenylacetic acid (DHAA) can improve gut barrier function by inhibiting the mitogen-activated protein kinase (MAPK) - myosin light-chain kinase (MLCK) signaling pathway in type 2 diabetes (T2D) mice. T2D mice were induced by a high-fat diet combined with streptozotocin. T2D mice were intragastrically administered with DHAA at 75 and 150 mg kg/bod ...
DOI:
10.1016/j.lfs.2022.120742

20. IL- 37 alleviates liver granuloma caused by Schistosoma japonicum infection by inducing alternative macrophage activation

Author:
Cuiping Ren; Fengchun Liu; Chen Xing; Ruyu Zhao; Xiaoxue Tang; Miao Liu; Wenda Gao; Jijia Shen
Source:
Parasites & vectors 2022 v.15 no.1 pp. 300
ISSN:
1756-3305
Subject:
AMP-activated protein kinase; Schistosoma japonicum; Western blotting; blood serum; coculture; enzyme-linked immunosorbent assay; fibrosis; granuloma; interleukins; liver; liver cirrhosis; liver function; macrophage activation; macrophages; mice; people; phenotype; phosphorylation; reverse transcriptase polymerase chain reaction; schistosomiasis
Abstract:
... BACKGROUND: Hepatic macrophages regulate liver granuloma formation and fibrosis caused by infection with Schistosoma japonicum, with the manner of regulation dependent on macrophage activation state. Interleukin (IL)-37 may have immunomodulatory effects on macrophages. However, whether IL-37 can affect liver granuloma formation and fibrosis by affecting the polarization of macrophages in S. japoni ...
DOI:
10.1186/s13071-022-05420-6