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1. (−)-Epicatechin modulates the expression of myomiRs implicated in exercise response in mouse skeletal muscle

Author:
Carlos Palma-Flores; Paola B. Zárate-Segura; J. Manuel Hernández-Hernández; Sergio de los Santos; Andrea S. Tejeda-Gómez; Luis Javier Cano-Martínez; Patricia Canto; Jorge Omar Garcia-Rebollar; Ramón M. Coral-Vázquez
Source:
Gene 2023 v.849 pp. 146907
ISSN:
0378-1119
Subject:
Western blotting; bioinformatics; exercise; genes; hypertrophy; mice; microRNA; muscle development; muscles; myogenin; signal transduction; skeletal muscle
Abstract:
... The flavanol (−)-epicatechin has exercise-mimetic properties. Besides, several miRNAs play a role in modulating the adaptation of the muscle to different training protocols. However, notwithstanding all information, few studies aimed to determine if (−)-epicatechin can modify the expression of miRNAs related to skeletal muscle development and regeneration. Mice were treated for fifteen days by ora ...
DOI:
10.1016/j.gene.2022.146907

2. 23-O-acetylshengmanol-3-O-α-L-arabinoside alleviates lipopolysaccharide-induced acute lung injury through inhibiting IκB/NF-κB and MAPK/AP-1 signaling pathways

Author:
Chunyan Chen; Lin Li; Xiaomei Liu; Deqin Zhang; Yang Liu; Yuhong Li
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115725
ISSN:
0378-8741
Subject:
Actaea cimicifuga; Oriental traditional medicine; Western blotting; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; fluorescent antibody technique; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; phosphorylation; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
DOI:
10.1016/j.jep.2022.115725

3. Ammonia-induced oxidative stress triggered proinflammatory response and apoptosis in pig lungs

Author:
Daojie Li; Long Shen; Di Zhang; Xiaotong Wang; Qiankun Wang; Wenhao Qin; Yun Gao; Xiaoping Li
Source:
Journal of environmental sciences (China) 2023 v.126 pp. 683-696
ISSN:
1001-0742
Subject:
DNA; Western blotting; ammonia; animal models; apoptosis; dose response; epithelium; genes; inflammation; lungs; metabolome; mineral fertilizers; oxidative phosphorylation; oxidative stress; protein synthesis; pyruvic acid; swine; toxicity; transcriptome; transmission electron microscopy; China
Abstract:
... Ammonia, a common toxic gas, is not only one of the main causes of haze, but also can enter respiratory tract and directly affect the health of humans and animals. Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung. In this study, the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using trans ...
DOI:
10.1016/j.jes.2022.05.005

4. Anti-inflammatory and osteoprotective effects of Shi-Wei-Ru-Xiang pills on collagen-induced arthritis in rats via inhibiting MAPK and STAT3 pathways

Author:
Hui Xiong; Fengping Meng; Miao Luo; Weiwu Chen; Juan Tian; Lunju Chen; Yankun Ju; Zhinan Mei
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115693
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Western blotting; animal models; apoptosis; blood serum; cartilage; chondrocytes; coculture; flow cytometry; inflammation; interleukin-6; phosphorylation; protein synthesis; rheumatoid arthritis
Abstract:
... Shi-Wei-Ru-Xiang pills (SW) as a tradition Tibetan medicine has been clinically proved effective in rheumatoid arthritis (RA) treatment. However, the underlying mechanism of SW remains unclear. This study aimed to investigate the anti-arthritic effect of SW and its possible mechanisms of action. A CIA rat model in vivo, and IL-1β-stimulated synoviocytes or chondrocytes and a co-culture system (IL- ...
DOI:
10.1016/j.jep.2022.115693

5. Atractylodes lancea and Magnolia officinalis combination protects against high fructose-impaired insulin signaling in glomerular podocytes through upregulating Sirt1 to inhibit p53-driven miR-221

Author:
Jie Yang; Zi-Xuan Wang; Lei Fang; Tu-Shuai Li; Zhi-Hong Liu; Ying Pan; Ling-Dong Kong
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115688
ISSN:
0378-8741
Subject:
Atractylodes lancea; Magnolia officinalis; Oriental traditional medicine; Western blotting; adiponectin receptors; agonists; animal models; diet; fluorescent antibody technique; fructose; humans; insulin; insulin resistance; kidneys; liquid chromatography; non-specific serine/threonine protein kinase; pharmacology; rats; tandem mass spectrometry; therapeutics; thermosensitivity
Abstract:
... In traditional Chinese medicine, a long term of improper diet causes the Dampness and disturbs Zang-Fu's functions including Kidney deficiency. Atractylodes lancea (Atr) and Magnolia officinalis (Mag) as a famous herb pair are commonly used to transform Dampness, with kidney protection. To explore how Atr and Mag protected against insulin signaling impairment in glomerular podocytes induced by hig ...
DOI:
10.1016/j.jep.2022.115688

6. Calcium pyruvate attenuates fat deposition by augmenting fatty acid oxidation and inhibiting glucose oxidation in juvenile large yellow croaker (Larimichthys crocea) consuming a high-fat diet

Author:
Yunqiang Zhang; Wencong Lai; Qiuchi Chen; Wei Fang; Kun Cui; Yuliang He; Xueshan Li; Kangsen Mai; Qinghui Ai
Source:
Aquaculture 2023 v.562 pp. 738778
ISSN:
0044-8486
Subject:
AMP-activated protein kinase; Larimichthys crocea; Western blotting; acetyl-CoA carboxylase; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; beta oxidation; blood glucose; calcium; carnitine palmitoyltransferase; catalase; citrates; diacylglycerol acyltransferase; dietary supplements; droplet size; fatty acid transport proteins; fatty-acid synthase; fish; gene expression; glucokinase; glucose; growth performance; hepatosomatic index; hexokinase; high density lipoprotein cholesterol; high fat diet; interleukin-1beta; juveniles; lipemic effect; lipogenesis; liver; low density lipoprotein cholesterol; malate dehydrogenase; muscles; obesity; oxidation; phosphofructokinases; prostaglandin synthase; protein synthesis; pyruvate dehydrogenase (lipoamide); pyruvate kinase; pyruvic acid; succinate dehydrogenase (quinone); triacylglycerol lipase; triacylglycerols; weight gain
Abstract:
... Calcium pyruvate (CP), known as an energetic aid, has been shown to enhance lipid utilization and prevent obesity in mammals. However, very few studies were conducted to evaluate the potential implications of CP for aquatic animals. This study examined the role of CP on fat deposition in large yellow croaker by feeding fish with high-fat diets containing four levels of dietary CP (0, 0.375%, 0.75% ...
DOI:
10.1016/j.aquaculture.2022.738778

7. Celastrus orbiculatus Thunb. extract inhibits EMT and metastasis of gastric cancer by regulating actin cytoskeleton remodeling

Author:
Haibo Wang; YuanYuan Luo; Shiya Ou; Tengyang Ni; Zewen Chu; Xinyi Feng; Xiaojun Dai; Xiaochun Zhang; Yanqing Liu
Source:
Journal of ethnopharmacology 2023 v.301 pp. 115737
ISSN:
0378-8741
Subject:
Celastrus orbiculatus; Oriental traditional medicine; Western blotting; actin; analgesics; animals; cell movement; medicinal plants; metalloproteinases; metastasis; microfilaments; neoplasm cells; protein degradation; regulatory proteins; stomach neoplasms; China
Abstract:
... The traditional Chinese medicine herb Celastrus orbiculatus Thunb. is an important folk medicinal plant in China that has been used as an anti-inflammatory, antitumor, and analgesic in various diseases. Recent years, many studies have reported the significant effects of Celastrus orbiculatus Thunb. extract (COE) on gastric cancer. However, the specific mechanism by which COE regulates gastric canc ...
DOI:
10.1016/j.jep.2022.115737

8. Core-shell SERS nanotags-based western blot

Author:
Bing Liu; Hanyu Tang; Qian Liu; Wenwen Wang; Haitao Li; Shiya Zheng; Fei Sun; Xiangwei Zhao
Source:
Talanta 2023 v.253 pp. 123888
ISSN:
0039-9140
Subject:
Western blotting; colorimetry; detection limit; glyceraldehyde-3-phosphate dehydrogenase; nanoparticles
Abstract:
... Western blot (WB) is the most commonly used scheme for protein identification in life science, but it still faces great challenges in the accurate quantitative detection of low-abundance proteins. Here, we proposed a novel surface-enhanced Raman scattering-based Western blot (SERS-WB) to solve this challenge. SERS nanotags were used as quantitative labels of proteins, which were composed of gold-s ...
DOI:
10.1016/j.talanta.2022.123888

9. Effects of breviscapine on cerebral ischemia-reperfusion injury and intestinal flora imbalance by regulating the TLR4/MyD88/NF-κB signaling pathway in rats

Author:
Hai-Dong Chen; Ming-Zhao Jiang; Ying-Ying Zhao; Xin Li; Hai Lan; Wan-Qi Yang; Yong Lai
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115691
ISSN:
0378-8741
Subject:
Erigeron breviscapus; Western blotting; active ingredients; animal models; blood serum; brain; dysbiosis; herbal medicines; infarction; intestinal microorganisms; intestines; ischemia; pain; paralysis; people; protective effect; rheumatoid arthritis; stroke; traditional medicine; China
Abstract:
... The plant Erigeron breviscapus (Vant.) Hand.-Mazz.,a Chinese herbal medicine with multiple pharmacological effects and clinical applications, has been traditionally used in the treatment of paralysis caused by stroke and joint pain from rheumatism by the Yi minority people of Southwest China for generations.However, its mechanism involves many factors and has not been fully clarified. Taking intes ...
DOI:
10.1016/j.jep.2022.115691

10. Hericium caput-medusae (Bull.:Fr.) Pers. fermentation concentrate polysaccharides improves intestinal bacteria by activating chloride channels and mucus secretion

Author:
Cong Zhao; Chang Sun; Jing Yuan; Ivan Steve Nguepi Tsopmejio; Yuting Li; Yu Jiang; Hui Song
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115721
ISSN:
0378-8741
Subject:
Enterobacter; Hericium; Lachnospiraceae; Ruminococcaceae; Staphylococcus; Western blotting; chlorides; cystic fibrosis transmembrane conductance regulator; digestive tract; drugs; edible fungi; fermentation; intestinal microorganisms; intestinal secretions; intestines; mechanism of action; mucus; polysaccharides; protective effect; protein synthesis; secretion; traditional medicine; China
Abstract:
... As a traditional edible fungus in China and many other Asian countries, Hericium caput-medusae (Bull. Fr.) Pers. is widely used to improve the health of the gastrointestinal tract. For example, the drug “Weilexin Granules” is mainly composed of H. caput-medusae (Bull. Fr.) Pers. fermentation concentrate. However, the mechanism of action remains to be elucidated. The purpose of this study was to as ...
DOI:
10.1016/j.jep.2022.115721

11. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
DNA; DNA damage; Sparus aurata; Western blotting; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

12. Saffron essential oil ameliorates CUMS-induced depression-like behavior in mice via the MAPK-CREB1-BDNF signaling pathway

Author:
Ziwei Chen; Jinping Gu; Susu Lin; Zijin Xu; Hongyu Xu; Jiajing Zhao; Peishi Feng; Yi Tao; Suhong Chen; Ping Wang
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115719
ISSN:
0378-8741
Subject:
Crocus sativus; Oriental traditional medicine; Western blotting; antidepressants; anxiety; blood serum; breathing; calcium; dopamine; enzyme-linked immunosorbent assay; essential oils; fluoxetine; gas chromatography-mass spectrometry; hippocampus; histopathology; models; neurons; odors; pharmacology; saffron; serotonin; stigma; sucrose; synapse; tail suspension test; therapeutics
Abstract:
... Saffron, the dried stigma of Crocus sativus L., has a long history of use in the treatment of depression in traditional Chinese medicine and Islamic medicine. The unique aroma of saffron, primarily derived from its volatile oil, has been widely used by folk to mitigate anxiety and depression via sniffing because the aroma of saffron has a pleasant and invigorating effect. Aim of the study: This st ...
DOI:
10.1016/j.jep.2022.115719

13. Uncovering the effect and mechanism of Panax notoginseng saponins on metabolic syndrome by network pharmacology strategy

Author:
Yisa Wang; Peng Ma; Zijing Wang; Mingxia Sun; Biyu Hou; Tianshu Xu; Wenlan Li; Xiuying Yang; Guanhua Du; Tengfei Ji; Guifen Qiang
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115680
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Panax notoginseng; Western blotting; adipocytes; adiposity; blood glucose; blood stasis; brown adipose tissue; computer simulation; drugs; energy; fatty liver; glucose; heat production; high fat diet; histopathology; insulin tolerance test; liver; metabolic diseases; metabolic syndrome; metabolism; obesity; pharmacology; protective effect; quantitative polymerase chain reaction; saponins; stomach; therapeutics; weight loss; white adipose tissue
Abstract:
... Metabolic syndrome (MetS) is a cluster of disease centered on obesity, which is the result of stagnation of liver qi according to traditional Chinese medicine. Panax notoginseng is a traditional Chinese herbal medicine, entering liver and stomach meridians and dissipating blood stasis, in which panax notoginseng saponins (PNS) are the main active components. However, its effects and mechanism on m ...
DOI:
10.1016/j.jep.2022.115680

14. Xianglian Pill attenuates ulcerative colitis through TLR4/MyD88/NF-κB signaling pathway

Author:
Yuxin Dai; Qiulu Lu; Peiyi Li; Junyu Zhu; Jiaxin Jiang; Tong Zhao; Yue Hu; Kang Ding; Min Zhao
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115690
ISSN:
0378-8741
Subject:
Oriental traditional medicine; Western blotting; blood serum; colon; dextran sulfate; enzyme activity; enzyme-linked immunosorbent assay; fluorescent antibody technique; histopathology; interleukin-18; interleukin-6; liquid chromatography; models; myeloperoxidase; necrosis; neoplasms; protein domains; quantitative polymerase chain reaction; salicylic acid; ubiquitination; ulcerative colitis
Abstract:
... Xianglian Pill (XLP) is a classical Chinese medicine prescription applied for controlling ulcerative colitis (UC). Whereas, the underlying mechanism remains unclear. The present work was aimed to investigate the mechanism of XLP in dextran sulfate sodium (DSS)-induced UC via the Toll Like Receptor 4 (TLR4)/Myeloid Differentiation factor 88 (MyD88)/Nuclear Factor kappa-B (NF-κB) signaling in mice.  ...
DOI:
10.1016/j.jep.2022.115690

15. The crude extract from the flowers of Trollius chinensis Bunge exerts anti-influenza virus effects through modulation of the TLR3 signaling pathway

Author:
Yuxi Liang; Xiaoli Liu; Jingyan Hu; Songli Huang; Xin Ma; Xiaoyan Liu; Rufeng Wang; Xiuhua Hu
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115743
ISSN:
0378-8741
Subject:
Influenza A virus; Oriental traditional medicine; Trollius chinensis; Western blotting; blood; cytoplasm; epithelium; fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
DOI:
10.1016/j.jep.2022.115743

16. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

17. (-) - Epicatechin improves Tibialis anterior muscle repair in CD1 mice with BaCl2-induced damage

Author:
Magally Ramírez-Ramírez; Francisca Fernández-Valverde; Andrea Reséndiz-García; Mirna G Martínez-Damas; Luis Javier Cano-Martínez; Alejandro Zentella-Dehesa; Ramón M Coral-Vázquez
Source:
Journal of nutritional biochemistry 2022 v.107 pp. 109069
ISSN:
0955-2863
Subject:
Western blotting; animal models; antioxidants; diabetes; epicatechin; histology; hypertrophy; mice; muscle development; muscles; muscular dystrophy; myogenin; sarcopenia; skeletal muscle; therapeutics
Abstract:
... Several studies have shown the beneficial effect that Epicatechin (Epi) has on the skeletal muscle of murine models and patients with muscular dystrophy and in the muscles of patients with diabetes or murine sarcopenia models. This flavanol has been shown to enhance antioxidant pathways and improve muscle architecture. However, the repair process during muscle regeneration has not been analyzed. T ...
DOI:
10.1016/j.jnutbio.2022.109069

18. (±)-Equol interferes with the secretion of gonadotropin-releasing hormone and the expression of neuromodulators in murine neurons

Author:
Jingyuan Xiong; Minghui Wang; Ye Tian; Aru Ling; Shufang Shan; Li Zhao; Guo Cheng
Source:
Toxicological and environmental chemistry 2022 v.104 no.1 pp. 129-140
ISSN:
1029-0486
Subject:
RING finger domains; Western blotting; cell viability; daidzein; environmental science; enzyme-linked immunosorbent assay; estrogens; females; gonadotropin-releasing hormone; intestines; menopause; metabolites; mice; neurotransmitters; quantitative polymerase chain reaction; secretion; toxicology
Abstract:
... S-Equol is an intestinal bacterial metabolite of the isoflavone daidzein. Racemic (±)-equol is used as a dietary estrogen supplement in menopausal women and is suggested to affect the female reproductive function and development, primarily controlled by the hypothalamic-pituitary-gonadal axis and gonadotropin-releasing hormone release. In this study, murine neurons were utilized to evaluate the ef ...
DOI:
10.1080/02772248.2022.2055031

19. (−)-Agelasidine A Induces Endoplasmic Reticulum Stress-Dependent Apoptosis in Human Hepatocellular Carcinoma

Author:
I-Ta Lu; Shih-Chao Lin; Yi-Chia Chu; Ya Wen; You-Cheng Lin; Wen-Chien Cheng; Jyh-Horng Sheu; Chi-Chien Lin
Source:
Marine drugs 2022 v.20 no.2 pp. -
ISSN:
1660-3397
Subject:
Western blotting; antibacterial properties; apoptosis; caspases; cell viability; death domain receptors; endoplasmic reticulum; guanidines; hepatoma; humans; liver; membrane potential; methanol; mitochondria; mitochondrial membrane; sesquiterpenoids
Abstract:
... Liver cancers, such as hepatocellular carcinoma (HCC), are a highly prevalent cause of cancer-related deaths. Current treatments to combat liver cancer are limited. (−)-Agelasidine A, a compound isolated from the methanol extract of Agelasnakamurai, a sesquiterpene guanidine derived from sea sponge, has antibacterial activity. We demonstrated its anticancer capabilities by researching the associat ...
DOI:
10.3390/md20020109

20. 13,13a-seco-protoberberines from the tubers of Corydalis yanhusuo and their anti-inflammatory activity

Author:
Gui-Yang Xia; Dong-Jie Fang; Ling-Yan Wang; Huan Xia; Ya-Nan Wang; Hong-Cai Shang; Sheng Lin
Source:
Phytochemistry 2022 v.194 pp. 113023
ISSN:
0031-9422
Subject:
Corydalis yanhusuo; Western blotting; active sites; anti-inflammatory activity; bioassays; enantiomers; inducible nitric oxide synthase; interleukin-6; lipopolysaccharides; molecular dynamics; nitric oxide; plant biochemistry; skeleton; spectral analysis; transcription factor NF-kappa B
Abstract:
... Six undescribed protoberberine derivatives including two pairs of enantiomers, named yanhusanines G-L, along with fifteen reported protoberberine alkaloids, were isolated from the tubers of Corydalis yanhusuo. Among them, yanhusanines H-L feature a unique 13,13a-seco skeleton which is rare in nature. Their structural elucidations were achieved by extensive spectroscopic analysis and quantum chemis ...
DOI:
10.1016/j.phytochem.2021.113023

21. 17β-estradiol modulates the expression of hormonal receptors on THP-1 T. gondii-infected macrophages and monocytes in an AKT and ERK-dependent manner

Author:
Ana Laura Pereira-Suárez; María de la Luz Galván-Ramírez; Laura Rocío Rodríguez-Pérez; Edgar I. López-Pulido; Christian David Hernández-Silva; Inocencia Guadalupe Ramírez-López; Grecia Viridiana Morales Amaya; Luis David Lopez Cabrera; José Francisco Muñoz-Valle; Adrián Ramírez-de-Arellano
Source:
Molecular and biochemical parasitology 2022 v.247 pp. 111433
ISSN:
0166-6851
Subject:
Toxoplasma gondii; Western blotting; cell growth; estrogen receptors; inflammation; macrophages; models; monocytes; parasites; parasitology; phosphatidylinositol 3-kinase; pregnancy; prolactin receptors; tachyzoites
Abstract:
... Toxoplasma gondii (T. gondii) is a parasite common in pregnancy. Monocytes and macrophages are a significant immunologic barrier against T. gondii by boosting up inflammation. This outcome is highly regulated by signaling pathways such as MAPK (ERK1/2) and PI3K (AKT), necessary in cell growth and proliferation. It may be associated with the hormonal receptors’ modulation by T. gondii (Estrogen Rec ...
DOI:
10.1016/j.molbiopara.2021.111433

22. 17β-estradiol reduces NF-κB expression induced by increased crosstalk between KLF5 and ERα in murine vascular smooth muscle cells

Author:
Man-li Zhang; Man-na Zhang; Wen-li Wang; Hui Chen; Xia Wang; Xuan Li; Li Li; Fei Tong
Source:
Biochemical and biophysical research communications 2022 v.625 pp. 181-187
ISSN:
0006-291X
Subject:
Western blotting; cardiovascular system; cell proliferation; chromatin immunoprecipitation; fluorescent antibody technique; inflammation; luciferase; research; smooth muscle
Abstract:
... Pathological vascular remodeling and cell proliferation play vital roles in many proliferative vascular diseases. Estrogen can protect the cardiovascular system, but its exact molecular mechanism is unknown. Here we report that 17β-estradiol (E2) suppressed vascular smooth muscle cells (VSMCs) proliferation and inflammation. qRT-PCR and Western blot demonstrated that E2 decreased NF-κB p50 express ...
DOI:
10.1016/j.bbrc.2022.07.054

23. 18β-Glycyrrhetinic acid altered the intestinal permeability in the human Caco-2 monolayer cell model

Author:
Mojtaba Malekinejad; Mohammad Reza Pashaee; Hassan Malekinejad
Source:
European journal of nutrition 2022 v.61 no.7 pp. 3437-3447
ISSN:
1436-6207
Subject:
Western blotting; absorption; adhesion; biochemical pathways; cell viability; cytotoxicity; dietary supplements; electrical resistance; epithelium; glycyrrhizin; human cell lines; humans; intestines; luciferase; metabolites; models; occludins; permeability; protein content; tight junctions; toxicity testing; traditional medicine
Abstract:
... PURPOSE: Glycyrrhizin (GL) and its metabolites 18α-glycyrrhetinic acid (18α-GA) and 18β-glycyrrhetinic acid (18β-GA) are used as traditional medicine and food sweeteners. As the major rout of their administration is oral way, therefore their impact on intestinal epithelial cells are investigated. METHODS: The effects of GL and its metabolites on cell viability using MTT assay, on cytotoxicity usin ...
DOI:
10.1007/s00394-022-02900-4

24. 2-Methoxyestradiol TPGS Micelles Attenuate Cyclosporine A-Induced Nephrotoxicity in Rats through Inhibition of TGF-β1 and p-ERK1/2 Axis

Author:
Mohammed W. Al-Rabia; Mohamed A. Alfaleh; Hani Z. Asfour; Waleed S. Alharbi; Mohamed A. El-Moselhy; Nabil A. Alhakamy; Usama A. Fahmy; Osama A. A. Ahmed; Omar Fahmy; Omar M. Rashad; Abdulmohsin J. Alamoudi; Ashraf B. Abdel-Naim
Source:
Antioxidants 2022 v.11 no.8 pp. -
ISSN:
2076-3921
Subject:
Western blotting; antioxidants; bioavailability; cyclosporine; kidneys; males; micelles; nanocarriers; nephrotoxicity; oral administration; particle size; polyethylene glycol; protein synthesis; succinic acid
Abstract:
... The immunosuppressant cyclosporine A (CSA) has been linked to serious renal toxic effects. Although 2-methoxyestradiol (2ME) possesses a wide range of pharmacological abilities, it suffers poor bioavailability after oral administration. The purpose of this study was to evaluate the potential of 2ME loaded D-ɑ-tocopheryl polyethylene glycol succinate (TPGS) micelles to prevent CSA-induced nephrotox ...
DOI:
10.3390/antiox11081499

25. 20 (S)-ginsenoside Rh2 inhibits colorectal cancer cell growth by suppressing the Axl signaling pathway in vitro and in vivo

Author:
Haibo Zhang; Jun-Koo Yi; Hai Huang; Sijun Park; Wookbong Kwon; Eungyung Kim; Soyoung Jang; Si-Yong Kim; Seong-kyoon Choi; Duhak Yoon; Sung-Hyun Kim; Kangdong Liu; Zigang Dong; Zae Young Ryoo; Myoung Ok Kim
Source:
Journal of Ginseng Research 2022 v.46 no.3 pp. 396-407
ISSN:
1226-8453
Subject:
Panax; Western blotting; antineoplastic activity; apoptosis; cell cycle checkpoints; cell growth; cell proliferation; colorectal neoplasms; morbidity; mortality; neoplasm cells; research; toxicity; transfection; xenotransplantation
Abstract:
... Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenoside Rh2 (G-Rh2) is a natural compound extracted from ginseng, which exhibits anticancer effects in many cancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo. Cell proliferation, migration, invasion, apoptosis, cell cycle, and western ...
DOI:
10.1016/j.jgr.2021.07.004

26. 3,4,5‐O‐tricaffeoylquinic acid alleviates ionizing radiation‐induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling

Author:
Jiajun Liu; Wen Hu; Xiaoying Ma; Xin Liang; Long Lin; Jianming Huang; Jianwen Liu
Source:
Environmental toxicology 2022 v.37 no.2 pp. 349-361
ISSN:
1520-4081
Subject:
Azolla; Western blotting; agonists; apoptosis; caspases; cell lines; cytotoxicity; digestive system; ecotoxicology; epithelial cells; immune system; membrane potential; mitochondria; radioprotective effect; radiotherapy
Abstract:
... Ionizing radiation (IR) brings many health problems to humans, causing damage to the digestive system, hematopoietic system, and immune system. Natural compounds derived from plants have attracted widespread attention due to their low toxicity. Here, we found that 3,4,5‐O‐tricaffeoylquinic acid (tCQA) extracted from natural plant Azolla imbricata could significantly alleviate the systemic damage i ...
DOI:
10.1002/tox.23403

27. 3,4-Dihydroxyphenylacetic acid ameliorates gut barrier dysfunction via regulation of MAPK-MLCK pathway in type 2 diabetes mice

Author:
Min Liu; Li Wang; Bijun Huang; Qun Lu; Rui Liu
Source:
Life sciences 2022 v.305 pp. 120742
ISSN:
0024-3205
Subject:
Western blotting; absorption barrier; anti-inflammatory activity; blood glucose; body weight; digestive system diseases; glutathione; high fat diet; hyperglycemia; inflammation; insulin; insulin resistance; interleukin-10; intestines; lipopolysaccharides; malondialdehyde; mitogen-activated protein kinase; myosin; noninsulin-dependent diabetes mellitus; oxidative stress; protein synthesis; streptozotocin; superoxide dismutase; tight junctions
Abstract:
... This study aims to investigate whether 3,4-dihydroxyphenylacetic acid (DHAA) can improve gut barrier function by inhibiting the mitogen-activated protein kinase (MAPK) - myosin light-chain kinase (MLCK) signaling pathway in type 2 diabetes (T2D) mice. T2D mice were induced by a high-fat diet combined with streptozotocin. T2D mice were intragastrically administered with DHAA at 75 and 150 mg kg/bod ...
DOI:
10.1016/j.lfs.2022.120742

28. IL- 37 alleviates liver granuloma caused by Schistosoma japonicum infection by inducing alternative macrophage activation

Author:
Cuiping Ren; Fengchun Liu; Chen Xing; Ruyu Zhao; Xiaoxue Tang; Miao Liu; Wenda Gao; Jijia Shen
Source:
Parasites & vectors 2022 v.15 no.1 pp. 300
ISSN:
1756-3305
Subject:
AMP-activated protein kinase; Schistosoma japonicum; Western blotting; blood serum; coculture; enzyme-linked immunosorbent assay; fibrosis; granuloma; interleukins; liver; liver cirrhosis; liver function; macrophage activation; macrophages; mice; people; phenotype; phosphorylation; reverse transcriptase polymerase chain reaction; schistosomiasis
Abstract:
... BACKGROUND: Hepatic macrophages regulate liver granuloma formation and fibrosis caused by infection with Schistosoma japonicum, with the manner of regulation dependent on macrophage activation state. Interleukin (IL)-37 may have immunomodulatory effects on macrophages. However, whether IL-37 can affect liver granuloma formation and fibrosis by affecting the polarization of macrophages in S. japoni ...
DOI:
10.1186/s13071-022-05420-6

29. 5-Methoxytryptophan pretreatment alleviates lipopolysaccharide-induced cardiac injury and dysfunction

Author:
Yang Fu; Yi-Fei Dong
Source:
Applied biological chemistry 2022 v.65 no.1 pp. 36
ISSN:
2468-0834
Subject:
Western blotting; apoptosis; biomarkers; cardiomyopathy; cardioprotective effect; echocardiography; fibroblasts; heart; inflammation; interleukin-10; interleukin-6; lipopolysaccharides; metabolites; protein content; therapeutics; tryptophan
Abstract:
... Reducing inflammation is a promising therapeutic approach for sepsis-induced cardiomyopathy (SIC). The 5-Methoxytryptophan (5-MTP) is a tryptophan metabolite that demonstrates anti-inflammatory, anti-fibrosis, anti-tumorigenesis, and anti-senescence features. Current investigations aimed to assess the 5-MTP pretreatment impacts on lipopolysaccharide (LPS)-induced cardiac injury and dysfunction. Fo ...
DOI:
10.1186/s13765-022-00705-x

30. 5‐Heptadecylresorcinol Protects against Atherosclerosis in Apolipoprotein E‐Deficient Mice by Modulating SIRT3 Signaling: The Possible Beneficial Effects of Whole Grain Consumption

Author:
Yiming Hao; Zihui Yang; Qing Li; Ziyuan Wang; Jie Liu; Jing Wang
Source:
Molecular nutrition & food research 2022 v.66 no.9 pp. e2101114
ISSN:
1613-4125
Subject:
Western blotting; apoptosis; atherosclerosis; biomarkers; blood serum; cholesterol; fluorescent antibody technique; food research; grain consumption; health claims; heart; high fat diet; histology; ingredients; mitochondria; risk; triacylglycerols; whole grain foods
Abstract:
... SCOPE: Whole grain consumption has been proven to be inversely associated with the risk of cardiovascular diseases. As a biomarker for whole grain dietary intake, 5‐heptadecylresorcinol (AR‐C17) has attracted increased attention due to its potential health‐improving activity. However, the beneficial effect of AR‐C17 on atherosclerosis prevention and the underlying mechanism remain unclear. METHODS ...
DOI:
10.1002/mnfr.202101114

31. 6-OH-BDE-47 inhibited proliferation of skin fibroblasts from pygmy killer whale by inducing cell cycle arrest

Author:
Tong Li; Yajing Sun; Ying Zeng; Edmond Sanganyado; Bo Liang; Wenhua Liu
Source:
Science of the total environment 2022 v.807 pp. 150561
ISSN:
0048-9697
Subject:
Orcinus orca; Western blotting; aquatic food webs; bioaccumulation; biphenyl; cell cycle checkpoints; cell proliferation; cyclin-dependent kinase; cyclins; environment; fibroblasts; hydroxylation; interphase; toxicity; toxicology
Abstract:
... Hydroxylated polybrominated diphenyl ethers (OH-BDEs) are major transformation products of PBDEs that readily bioaccumulate in the marine food web. Although 6-OH-BDE-47 is frequently and abundantly detected in cetaceans, its potential toxic effects are largely unknown. We explored the toxicological pathways and mechanisms of OH-BDEs by exposing pygmy killer whale skin fibroblast cell lines (PKW-LW ...
DOI:
10.1016/j.scitotenv.2021.150561

32. 6-methoxydihydroavicine, the alkaloid extracted from Macleaya cordata (Willd.) R. Br. (Papaveraceae), triggers RIPK1/Caspase-dependent cell death in pancreatic cancer cells through the disruption of oxaloacetic acid metabolism and accumulation of reactive oxygen species

Author:
Nengfang Ma; Fugen Shangguan; Hongfei Zhou; Huimin Huang; Jun Lei; Jing An; Guihua Jin; Weiwei Zhuang; Shipeng Zhou; Shijia Wu; Hongping Xia; Hailong Yang; Linhua Lan
Source:
Phytomedicine 2022 v.102 pp. 154164
ISSN:
0944-7113
Subject:
Macleaya cordata; Western blotting; alkaloids; apoptosis; caspases; cell growth; energy metabolism; flow cytometry; glycolysis; homeostasis; mitochondria; mitophagy; neoplasm cells; oxaloacetic acid; pancreatic neoplasms; reactive oxygen species
Abstract:
... Many extracts and purified alkaloids of M. cordata (Papaveraceae family) have been reported to display promising anti-tumor effects by inhibiting cancer cell growth and inducing apoptosis in many cancer types. However, no evidence currently exists for anti-pancreatic cancer activity of alkaloids extracted from M. cordata, including a novel alkaloid named 6‑methoxy dihydrosphingosine (6-Methoxydihy ...
DOI:
10.1016/j.phymed.2022.154164

33. 7,8-Dihydroxyflavone attenuates the virulence of Staphylococcus aureus by inhibiting alpha-hemolysin

Author:
Nan Bian; Xiangqian Chen; Xinran Ren; Zishu Yu; Mengli Jin; Xiaoyu Chen; Chang Liu; Yanhe Luan; Lin Wei; Ying Chen; Wu Song; Yicheng Zhao; Bingmei Wang; Tao Jiang; Chi Zhang; Zunhua Shu; Xin Su; Li Wang
Source:
World journal of microbiology & biotechnology 2022 v.38 no.11 pp. 200
ISSN:
0959-3993
Subject:
Staphylococcus aureus; Western blotting; animals; biotechnology; cytotoxicity; genes; hemolysis; hospitals; microbiology; synergism; therapeutics; transcription factors; vancomycin; viability; virulence
Abstract:
... Staphylococcus aureus (S. aureus), a Gram-positive bacteria, is an incurable cause of hospital and community-acquired infections. Inhibition bacterial virulence is a viable strategy against S. aureus infections based on the multiple virulence factors secreted by S. aureus. Alpha-hemolysin (Hla) plays a crucial role in bacteria virulence without affecting bacterial viability. Here, we identified th ...
DOI:
10.1007/s11274-022-03378-2

34. ABCA8 inhibits breast cancer cell proliferation by regulating the AMP activated protein kinase/mammalian target of rapamycin signaling pathway

Author:
Chunliu Lv; Han Yang; Jinsong Yu; Xiaowei Dai
Source:
Environmental toxicology 2022 v.37 no.6 pp. 1423-1431
ISSN:
1520-4081
Subject:
Western blotting; breast neoplasms; carcinogenesis; cell growth; cell proliferation; data collection; ecotoxicology; gene expression regulation; mammals; neoplasm cells; prognosis; protein kinases; rapamycin
Abstract:
... ATP‐binding cassette (ABC) subfamily A member 8 (ABCA8) has been reported to play a vital role in cancer development. Our study aimed to explore the role and the molecular mechanism of ABCA8 in breast cancer (BC) progression. GSE65194, GSE15852, and GSE45827 datasets were used to identify differentially expressed genes (DEGs) in BC. The diagnosis and prognosis value were determined using ROC curve ...
DOI:
10.1002/tox.23495

35. ACSL4 promotes colorectal cancer and is a potential therapeutic target of emodin

Author:
Guoliang Dai; Dong Wang; Shitang Ma; Shengwei Hong; Kang Ding; Xiying Tan; Wenzheng Ju
Source:
Phytomedicine 2022 v.102 pp. 154149
ISSN:
0944-7113
Subject:
Rheum palmatum; Western blotting; antineoplastic activity; carcinogenesis; cell proliferation; colorectal neoplasms; data collection; databases; emodin; gene expression regulation; immunohistochemistry; ingredients; mice; mortality; secretion; therapeutics; vascular endothelial growth factor receptor-1; vascular endothelial growth factor receptor-2
Abstract:
... Colorectal cancer (CRC) is an important death-related disease in the world and new therapeutic strategies are urgently needed to reduce mortality. Several studies have demonstrated that emodin, the main ingredient of Rheum palmatum, fights cancer but its potential anti-tumor effect on CRC is still unknown. The present study is aimed to explore the potential anti-tumor effects of emodin against CRC ...
DOI:
10.1016/j.phymed.2022.154149

36. ADAMTS8 inhibited lung cancer progression through suppressing VEGFA

Author:
Yutian Zhang; Kang Hu; Ziyi Qu; Zhihong Xie; Fei Tian
Source:
Biochemical and biophysical research communications 2022 v.598 pp. 1-8
ISSN:
0006-291X
Subject:
Western blotting; apoptosis; cell proliferation; epithelial cells; flow cytometry; gene expression regulation; immunohistochemistry; lung neoplasms; lungs; neoplasm cells; neoplasm progression; plasmids; research; survival rate; transfection; xenotransplantation
Abstract:
... ADAMTS8 expression has been identified to be low in many cancers including lung cancer. However, the specific functions and regulatory system of ADAMTS8 remain to be unveiled. To study the potential modulatory mechanism of ADAMTS8 in lung cancer in cell and xenograft mice models. Differential expression of ADAMTS8 in lung cancer was analyzed on online tools. So was the overall survival curve in as ...
DOI:
10.1016/j.bbrc.2022.01.110

37. AICAR stimulates mitochondrial biogenesis and BCAA catabolic enzyme expression in C2C12 myotubes

Author:
Jason S. Hinkle; Caroline N. Rivera; Roger A. Vaughan
Source:
Biochimie 2022 v.195 pp. 77-85
ISSN:
0300-9084
Subject:
Western blotting; acidification; amino acids; biogenesis; blood; catabolism; gene expression; genes; glycolysis; insulin resistance; keto acids; metabolites; mitochondria; models; myotubes; noninsulin-dependent diabetes mellitus; oxidoreductases; oxygen consumption; peroxisome proliferator-activated receptor gamma; protein kinases; protein synthesis; skeletal muscle
Abstract:
... Type 2 diabetes is characterized by reduced insulin sensitivity, elevated blood metabolites, and reduced mitochondrial metabolism. Insulin resistant populations often exhibit reduced expression of genes governing mitochondrial metabolism such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α). Interestingly, PGC-1α regulates the expression of branched-chain amino acid ...
DOI:
10.1016/j.biochi.2021.11.004

38. ALDH6A1 weakens the progression of colon cancer via modulating the RAS/RAF/MEK/ERK pathway in cancer cell lines

Author:
Xiang Li; Nan Wang; Yutong Wu; Yidan Liu; Ruoyu Wang
Source:
Gene 2022 v.842 pp. 146757
ISSN:
0378-1119
Subject:
Western blotting; adenocarcinoma; aldehyde dehydrogenase; apoptosis; colon; colorectal neoplasms; fibrosarcoma; genes; metalloproteinases; neoplasm cells; pathogenesis; quantitative polymerase chain reaction; rats; transfection; viruses
Abstract:
... Aldehyde dehydrogenase 6 family member A1 (ALDH6A1) is associated with multiple diseases, but its pathogenesis in colon cancer (CC) is ambiguous and needs further study so that this research explores the function of ALDH6A1 in CC. The level of ALDH6A1 in colon adenocarcinoma (COAD), CC tissues, and cells was measured by starBase v2.0, quantitative real-time polymerase chain reaction (qRT-PCR), and ...
DOI:
10.1016/j.gene.2022.146757

39. ASPN Is a Potential Biomarker and Associated with Immune Infiltration in Endometriosis

Author:
Li Wang; Jing Sun
Source:
Genes 2022 v.13 no.8 pp. -
ISSN:
2073-4425
Subject:
B-lymphocytes; T-lymphocytes; Western blotting; biomarkers; calcium; chemokines; cytotoxicity; data collection; databases; endometriosis; endometrium; endothelial cells; extracellular matrix; female genital diseases; fibroblasts; gene expression; gene expression regulation; genes; immunotherapy; metastasis; muscle contraction; patients; regression analysis; smooth muscle
Abstract:
... Objective: Endometriosis is a benign gynecological disease characterized by distant metastasis. Previous studies have discovered abnormal numbers and function of immune cells in endometriotic lesions. We aimed to find potential biomarkers of endometriosis and to explore the relationship between ASPN and the immune microenvironment of endometriosis. Methods: We obtained the GSE141549 and GSE7305 da ...
DOI:
10.3390/genes13081352

40. ASPP2 reduction attenuates HBV induced chronic liver damage: A hybrid mouse model study

Author:
Yang Wang; Yang Che; ShanShan Wang; JinMing Wang; Xiaoni Liu; Buxin Kou; Yuanyue Guan; Dexi Chen; Ying Shi
Source:
Biochemical and biophysical research communications 2022 v.610 pp. 61-69
ISSN:
0006-291X
Subject:
Hepatitis B virus; Western blotting; apoptosis; autophagy; blood serum; eosin; humans; hybrids; immune system; immunohistochemistry; inflammation; innate immunity; liver; metabolism; mice; necrosis; progeny; research; transcriptome; transcriptomics
Abstract:
... P53 Apoptosis Stimulating Protein 2 (ASPP2) is confirmed to participate in cellular activities including apoptosis, proliferation, autophagy, injury and so on. However, the role of ASPP2 in Hepatitis B virus (HBV) infection has not been reported in detail. The study explored the role of ASPP2 in HBV induced chronic liver damage. Transcriptome profiling of ASPP2-konckdown mouse liver were analyzed ...
DOI:
10.1016/j.bbrc.2022.03.109

41. ATP6V1H deficiency impairs glucose tolerance by augmenting endoplasmic reticulum stress in high fat diet fed mice

Author:
Shaoqing Yang; Yuzhuan Hou; Hengwei Zhang; Ying Hao; Yanli Zhang; Zanyan Zhao; Wenyan Ruan; Xiaohong Duan
Source:
Archives of biochemistry and biophysics 2022 v.716 pp. 109116
ISSN:
0003-9861
Subject:
H-transporting ATP synthase; Western blotting; biophysics; diabetes; endoplasmic reticulum stress; glucose; glucose tolerance; high fat diet; insulin; insulin secretion; models; proton pump; transcriptome; transportation; vacuoles
Abstract:
... Vacuolar H⁺-ATPase (V-ATPase) is a ubiquitous proton pump that mediates the proton transmembrane transportation in various cells. Previously, H subunit of V-ATPase (ATP6V1H) was found to be related with insulin secretion and diabetes. However, the mechanism by which ATP6V1H regulates insulin secretion and glucose metabolism remains unclear. Herein, we established a high-fat-diet (HFD) fed model wi ...
DOI:
10.1016/j.abb.2022.109116

42. ATX/LPA axis regulates FAK activation, cell proliferation, apoptosis, and motility in human pancreatic cancer cells

Author:
Yan Liao; Lei Liu; Jiayao Yang; Zhaohong Shi
Source:
In vitro cellular & developmental biology 2022 v.58 no.4 pp. 307-315
ISSN:
1071-2690
Subject:
Western blotting; adhesion; apoptosis; autocrine signaling; carcinogenesis; cell membranes; cell proliferation; cell viability; cultured cells; flow cytometry; fluorescent antibody technique; humans; lysophospholipids; non-specific protein-tyrosine kinase; pancreatic neoplasms; protein synthesis; secretion
Abstract:
... Previous studies implicated ATX/LPA axis as a potential driver of tumorigenesis and progression in pancreatic cancer. This study aimed to determine the existence of the autocrine pathway of ATX/LPA action in pancreatic cancer cells, and to elucidate its influence on focal adhesion kinase (FAK) activation, cellular proliferation, apoptosis, and migration. Firstly, we identified the lysophosphatidic ...
DOI:
10.1007/s11626-022-00660-3

43. Abnormal Expression of microRNA-296-3p in Type 2 Diabetes Patients and its Role in Pancreatic β-Cells Function by Targeting Tensin Homolog Deleted on Chromosome Ten

Author:
Minggang Cheng; Yichen Guo; Weichuan Zhong; Xueyan Chen; Guangzhou Guo
Source:
Biochemical genetics 2022 v.60 no.1 pp. 39-53
ISSN:
0006-2928
Subject:
Western blotting; apoptosis; blood glucose; blood serum; cell proliferation; cell viability; chromosomes; flow cytometry; insulin; insulin secretion; noninsulin-dependent diabetes mellitus; protective effect; reverse transcriptase polymerase chain reaction; therapeutics; transfection; uric acid
Abstract:
... Diabetes mellitus (DM), a familiar disease, is characterized by high blood glucose levels owing to insulin deficiency. Researches have suggested that the incidence rate of diabetes is increasing and it has become an important global epidemic. The type 2 diabetes mellitus (T2DM) is featured with pancreatic β-cell loss and lack of insulin release. Nevertheless, the therapeutic methods that was helpf ...
DOI:
10.1007/s10528-021-10083-6

44. Activation of PGC‐1α via isoliquiritigenin‐induced downregulation of miR‐138‐5p alleviates nonalcoholic fatty liver disease

Author:
Lu Wang; Xiaohui Wang; Lina Kong; Yingying Li; Kai Huang; Jingjing Wu; Changyuan Wang; Huijun Sun; Pengyuan Sun; Jiangning Gu; Haifeng Luo; Kexin Liu; Qiang Meng
Source:
Phytotherapy research 2022 v.36 no.2 pp. 899-913
ISSN:
0951-418X
Subject:
Western blotting; bioinformatics; drugs; fatty liver; fluorescent antibody technique; histopathology; inflammation; interleukin-6; lipid metabolism; lipids; liver; luciferase; metabolic diseases; phytotherapy; quantitative polymerase chain reaction; reactive oxygen species; research
Abstract:
... Nonalcoholic fatty liver disease (NAFLD), a metabolic disease, has received wide attention worldwide. However, there is no approved effective drug for NAFLD treatment. In the study, H&E and Oil Red O staining were employed to detect liver histopathological changes and the accumulation of lipid droplets. Quantitative real‐time PCR, Western blot, bioinformatics, luciferase assay, immunofluorescence ...
DOI:
10.1002/ptr.7334

45. Activation of mitochondrial-associated apoptosis signaling pathway and inhibition of PI3K/Akt/mTOR signaling pathway by voacamine suppress breast cancer progression

Author:
Yi Zuo; Chao-zheng Zhang; Qing Ren; Yao Chen; Xiao Li; Ji-rui Yang; Hong-xiang Li; Wen-tao Tang; Hing-Man HO; Chen Sun; Mei-mei Li; Bo Ren; Yun Deng; Mao-lin Wang; Jun Lu
Source:
Phytomedicine 2022 v.99 pp. 154015
ISSN:
0944-7113
Subject:
Voacanga africana; Western blotting; alkaloids; apoptosis; breast neoplasms; cell proliferation; cyclin-dependent kinase; cyclins; flow cytometry; humans; interphase; membrane potential; mice; mitochondrial membrane; morbidity; mortality; neoplasm cells; neoplasm progression; protein synthesis; reactive oxygen species; toxicity
Abstract:
... Breast cancer is one of the malignant tumors with the highest morbidity and mortality rate. Numerous efficient anti-breast cancer drugs are being derived from the development of natural products. Voacamine (VOA), a bisindole alkaloid isolated from Voacanga africana Stapf, possesses various pharmacological and biological activities. In this study, we investigated the efficacy of VOA against breast ...
DOI:
10.1016/j.phymed.2022.154015

46. Active legumain promotes invasion and migration of neuroblastoma by regulating epithelial-mesenchymal transition

Author:
Min Zhang; Jianhua Zhu; Wei Wang; Zhiteng Jiang
Source:
Open life sciences 2022 v.17 no.1 pp. 676-685
ISSN:
2391-5412
Subject:
Western blotting; asparagine; cadherins; gene amplification; gene targeting; genes; humans; legumain; metastasis; neoplasms; slugs; transfection; vimentin
Abstract:
... Neuroblastoma (NB) is a commonly occurring malignancy in children. Epithelial-mesenchymal transition (EMT) is an adaptive change in promoting tumor metastasis. As an important factor in regulating tumor metastasis, whether legumain could promote metastasis of NB by EMT is still unexplored. Legumain is the active form of prolegumain, abundant in tumor plasma. So in the current study, different form ...
DOI:
10.1515/biol-2022-0012

47. Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells

Author:
Lingli Chen; Yuye Liu; Penghuan Jia; Hongli Zhang; Zhihong Yin; Dongfang Hu; Hongmei Ning; Yaming Ge
Source:
Environmental science and pollution research 2022 v.29 no.39 pp. 58927-58935
ISSN:
0944-1344
Subject:
Western blotting; axons; cell membranes; cerebral cortex; dose response; females; heavy metals; hippocampus; lead acetate; memory; mice; microscopy; nerve tissue; neurotoxicity; permeability; pollution; protein synthesis; research; survival rate
Abstract:
... Lead (Pb) is a widespread environmental heavy metal that can damage the cerebral cortex and hippocampus, and reduce the learning and memory ability in humans and animals. In vivo and in vitro models of acute lead acetate exposure were established to further study the mechanism of neurons injury. In this study, 4-week-old female Kunming mice were randomly divided into four groups. Each group was tr ...
DOI:
10.1007/s11356-022-20051-1

48. Adiponectin affects uterine steroidogenesis during early pregnancy and the oestrous cycle: An in vitro study

Author:
M. Kiezun; K. Dobrzyn; E. Zaobidna; E. Rytelewska; K. Kisielewska; M. Gudelska; K. Orzechowska; G. Kopij; K. Szymanska; B. Kaminska; T. Kaminski; N. Smolinska
Source:
Animal reproduction science 2022 v.245 pp. 107067
ISSN:
0378-4320
Subject:
Western blotting; adiponectin; endometrium; energy balance; estradiol; estrone; estrous cycle; gene expression; genes; insulin; myometrium; pregnancy; protein synthesis; radioimmunoassays; secretion; steroidogenesis; swine; testosterone
Abstract:
... Reproduction in females is an energetically demanding process. We assumed that adiponectin (ADPN), known for its role in energy balance maintenance, is also engaged in the regulation of uterine steroidogenesis in the pig. We determined the impact of ADPN alone or in combination with insulin (INS) on testosterone (T), estrone (E₁) and estradiol (E₂) secretion by porcine endometrium and myometrium, ...
DOI:
10.1016/j.anireprosci.2022.107067

49. Aerobic exercise improves adipogenesis in diet-induced obese mice via the lncSRA/p38/JNK/PPARγ pathway

Author:
Baoai Wu; Jiyuan Ding; Anping Chen; Yicun Song; Cong Xu; Fujun Tian; Jinfeng Zhao
Source:
Nutrition research 2022 v.105 pp. 20-32
ISSN:
0271-5317
Subject:
Western blotting; adipogenesis; adiposity; blood lipids; epididymis; exercise; lipid metabolism; lipids; mitogen-activated protein kinase; nutrition research; obesity; risk factors; spectrophotometers; viruses; white adipose tissue
Abstract:
... Adipogenesis is one of the triggers of obesity, which is a risk factor for various metabolic diseases. Long noncoding RNA steroid receptor RNA activator (lncRNA-SRA) is closely related to adipogenesis and p38/JNK mitogen-activated protein kinase mediates lipid production by regulating peroxisome proliferator-activated receptor gamma (PPARγ). Aerobic exercise can be efficient in improving adiposity ...
DOI:
10.1016/j.nutres.2022.04.004

50. Aerobic exercise improves intestinal mucosal barrier dysfunction through TLR4/MyD88/NF-κB signaling pathway in diabetic rats

Author:
Yuzhen Wu; Xia Liu; Jianping Li; Wei Ji; Qinghua Tian; Shichen Li
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
Toll-like receptor 4; Western blotting; blood glucose; blood serum; diabetes; diamine oxidase; exercise; insulin; insulin resistance; interleukin-6; intestinal mucosa; lipopolysaccharides; models; necrosis; neoplasms; occludins; research; transmission electron microscopy; villi
Abstract:
... This study examined the effects of aerobic exercise on the intestinal mucosal barrier dysfunction in diabetic rats. We established a diabetic rats model consisting of three groups: normal control (NC), diabetes control (DC), and diabetes eight-week aerobic exercise (DE). We measured serum fasting blood glucose (FBG), insulin (INS), diamine oxidase (DAO), D(-)-lactate (D-Lac), lipopolysaccharide (L ...
DOI:
10.1016/j.bbrc.2022.09.075