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... The objective of this work was to evaluate the influence of bread with thermal treated lentil flour on metabolic and physiological parameters. Thirty-two Wistar rats fed for 60 days with different diets were studied: control (C), wheat bread (WB), wheat-lentil bread (LB), and lentil flour (LF). Food intake, weight gain, feces moisture, and apparent calcium absorption (AACa) were recorded during th ...
... Short-chain fatty acids (SCFAs) play key roles in maintaining health and treating disease. Quantification of important fecal SCFAs is necessary to facilitate the clarification of their biological roles. However, the existing quantifying methods mainly depend on complicated precolumn derivatization, and/or are unable to determine formic acid, a SCFA commonly associated with toxicity. In this study, ...
... Short-chain fatty acids (SCFAs) are gut microbiota metabolites recognized for their beneficial effects on the host organism. In this study, a simple and rapid sample preparation method combined to SCFAs analysis by direct injection and gas chromatography coupled with flame ionization detection (GC-FID), for the determination and quantification of eight SCFAs (acetic, propionic, i-butyric, butyric, ...
... The present study aimed to evaluate the fermentation of the glucogalactan kefiran by human intestinal microbiota, and to assess the biological effect triggered by the produced metabolites (butyrate and others). For this purpose, an in vitro fermentation model with faeces belonging to ten (10) children aged from 4 months to 3 years-old was used. Short-chain fatty acids (SCFA) and lactic acid produc ...
acidification; detection limit; feces; gas chromatography; mice; rapid methods; short chain fatty acids
Abstract:
... An increasing number of researchers are focusing on short-chain fatty acids (SCFAs), which are closely related to various aspects of the human body. This study presented a pretreatment method for mouse feces that combined acidification and extraction. Then, a direct injection process into gas chromatography was developed. The limits of detection using this method are 0.5–0.8 mg L⁻¹, and the corres ...
... Microbial characterization of two Italian traditional cheeses, Giuncata and Caciotta Leccese, was carried out, with the aim to isolate autochthonous bacterial strains to be used as starters to improve and standardize the quality of these cheeses. More than 400 bacterial isolates were found, using PCR-based identification, to belong to 12 species of the Streptococcus, Lactococcus, Lactobacillus, an ...
... Prebiotic supplements are used to promote gastrointestinal health by stimulating beneficial bacteria. The aim of this study was to compare the potential prebiotic effects of fructans with increasing degrees of polymerization, namely fructooligosaccharides (FOS) and inulins with a low and high polymerization degree (LPDI and HPDI, respectively), using an ex vivo fermentation system to simulate the ...
... AIMS: This study aimed to determine whether host‐adapted lactobacilli exhibit superior survival during intestinal transit relative to nomadic and free‐living organisms, and to characterize the impact of probiotic lactobacilli on autochthonous lactobacilli. METHODS AND RESULTS: Mixed cultures of Lactobacillus casei K9‐1 and Lactobacillus fermentum K9‐2, or reutericyclin producing Lactobacillus reut ...
... In developing countries dung cakes combustion as a household cooking fuel is a common practice; however this traditional fuel comes along with related environmental issues. The objective of this study was to assess the environmental impacts of the substitution of dung combustion by biogas systems in rural Ethiopia. For this purpose, the method of life cycle assessment was applied according to ISO ...
... This study aimed at assessing the utilisation possibility of Enterococcus faecalis as starter cultures for production of fermented sausages. The E. faecalis isolates isolated from faeces samples of 5‐day‐old healthy newborn babies, identified by 16SrDNA sequencing and free from virulence determinants as proven by genomic sequencing, were used for fermented sausages production as starter culture. S ...
... Deposition of urine and dung in pasture‐based livestock production systems is a major source of ammonia (NH₃) volatilization, contributing to the eutrophication and acidification of water bodies and to indirect nitrous oxide emissions. The objectives of this study were to (i) measure NH₃ volatilization from dung and urine in three seasons, (ii) test the effect of spiking urine with the nitrificati ...
... Ocean acidification (OA) caused by excessive CO₂ is a potential ecological threat to marine organisms. The impacts of OA on echinoderms are well-documented, but there has been a strong bias towards sea urchins, and limited information is available on sea cucumbers. This work examined the effect of medium-term (60 days) exposure to three pH levels (pH 8.06, 7.72, and 7.41, covering present and futu ...
... Anaerobic co-digestion (A co-D) performance of Phragmites australis, feces and kitchen waste with addition of clinoptilolite (one main kind of zeolite) was investigated to evaluate the improvement of biogas/methane production and internal mechanism of nitrogen and organics control. A better biogas/methane production was observed by 10% clinoptilolite (v/v) than bentonite and diatomite, with the sh ...
acidification; developing countries; environmental impact; environmental performance; eutrophication; feces; global warming; life cycle assessment; management systems; mineral fertilizers; municipal wastewater; nutrients; sanitation; urine; villages; wastewater treatment; water shortages; China
Abstract:
... To address issues of poor sanitation and water scarcity in developing countries, innovative wastewater management systems should be proposed, based on considerations of life cycle and local environmental impacts. Source-separation separates urine, feces, and gray water, enhancing the reuse of nutrients, and is considered a promising wastewater treatment method. This study evaluates the life cycle ...
... Management of animal manure in livestock and crop production is a major cause of nitrogen (N), phosphorus (P), and carbon (C) loss. The losses of N, P, and C contribute to adverse environmental impacts, such as climate change, terrestrial acidification, and marine eutrophication. Manure management technologies to reduce losses and impacts have been developed, but often focus on a single compound o ...
... Manure management contributes to adverse environmental impacts through losses of nitrogen (N), phosphorus, and carbon (C). In this study, we aimed to assess the potential of newly designed strategies for integrated manure management (IS) to reduce environmental impact. An important aspect of the strategies was that they prevented pollution swapping. Life cycle assessment was used to compute climat ...
... Fermented foods have a great significance since they provide and preserve large quantities of nutritious foods in a wide diversity of flavors, aromas and texture, which enrich the human diet. Originally fermented milks were developed as a means of preserving nutrients and are the most representatives of the category. The first aim of this study was to screen the effect of buckwheat flour and oat b ...
in vivo studies; oral vaccination; acidification; reproduction; ileum; organic acids and salts; feed supplements; tonsils; Salmonella Typhimurium; cecum; confidence interval; feces; control methods; lymph nodes; drinking water; blood; swine
Abstract:
... Despite current control measures, Salmonella in pigs remains a major public health concern. In this in vivo study, the effect of three intervention strategies on Salmonella Typhimurium transmission in pigs was evaluated. The first intervention was feed supplemented with coated calcium-butyrate (group A); the second comprised oral vaccination with a double-attenuated Salmonella Typhimurium strain ( ...
... Gaseous emissions from in-house storage of liquid animal manure remain a major contributor to the environmental impact of manure management. Our aim was to assess the life cycle environmental consequences and reduction potential of segregating fattening pig urine and feces with an innovative V-belt system and to compare it to conventional liquid manure management, that is, the reference. Moreover, ...
... Development of an analytical method with appropriate combination of extraction and quantification approaches for undigested phytate in ruminant feces and digesta will advance knowledge of phytate degradation in ruminants and help to reduce phosphorus excretion. Established quantification methods give satisfactory results for feedstuffs and nonruminant manure but recovery of phytate is incomplete f ...
... Benzoic acid (BA) in diets for growing pigs results in urinary acidification and reduced ammonia emission. The objective was to study the impact of BA supplementation on the acid-base status and mineral metabolism in pigs. Eight female 50-kg pigs, fitted with a catheter in the abdominal aorta, were allocated to one of 3 levels of BA: 0% (w/w) (control diet; n=3), 1% (n=3) or 2% (n=2). The experime ...
... Six Holstein heifers (body weight=535–625kg) fed a total mixed ration containing either high protein (13.4%) or low protein (9.0%) were used to evaluate the effect of 3 urine collection methods (chilled, acidified before collection, or acidified after 6h of collection) on urinary N preservation. In a 2-period crossover design, 16-d diet adjustment stages preceded five 24-h collections. Urinary cat ...
... Benzoic acid (BA) reduces pH of urine and thereby reduces the emission of ammonia and possibly also odorous sulphur-compounds from slurry. The effect of BA on mineral metabolism in growing pigs is not clear. The objective was therefore to study the effect of BA and methionine (Met) as a sulphur (S) source in diets for pigs on urine acidification and mineral metabolism. Twenty-four 45kg pigs in a 2 ...
... Ammonia emitted from manure can have detrimental effects on health, environmental quality, and fertilizer value. The objective of this study was to measure the potential for reduction in ammonia volatilization from swine (Sus scrofa domestica) manure by temperature control, stirring, addition of nitrogen binder (Mohave yucca, Yucca schidigera Roezl ex Ortgies) or urease inhibitor [N-(n-butyl) thio ...
... SUMMARY: The influence of a high-fat diet on the calcium absorption by growing dogs was determined in a crossover study with five 6-month-old beagles fed two diets with different fat contents. The dogs were fed a pelleted control diet (11 g fat/100 g diet) and a high-fat diet (33 g fat/100 g diet) according to the energy requirement for growing dogs. The high-fat diet was formulated by isoenergeti ...