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- Linga R. Gutha; Divakar Purushottam; Aruna Veeramachaneni; Sarita Tigulla; Vikas Kodappully; Chandana Enjala; Hitendrasinh Rajput; Jennifer Anderson; Bonnie Hong; Jean Schmidt; Shveta Bagga
- Transgenic research 2018 v.27 no.4 pp. 331-342
- actin; corn; crops; developmental stages; enzyme-linked immunosorbent assay; field experimentation; genotype; glyceraldehyde-3-phosphate dehydrogenase; growing season; hybrids; leaves; models; peptide elongation factors; plant tissues; safety assessment; transgenic plants; India
- ... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
- Vanessa Galli; Rafael da Silva Messias; Sérgio Delmar dos Anjos e Silva; Cesar Valmor Rombaldi
- Plant cell reports 2013 v.32 no.12 pp. 1869-1877
- actin; biofortification; carotenoids; children; computer software; corn; developmental stages; gene expression; genes; glyceraldehyde-3-phosphate dehydrogenase; landraces; metabolic engineering; phytoene synthase; quantitative polymerase chain reaction; seed development; surveys; tubulin; vitamin deficiencies
- ... KEY MESSAGE : The stability of candidate reference genes was evaluated in maize landrace varieties and during multiple grain developmental stages to evaluate the expression of carotenoid-related genes by RT-qPCR for application to maize biofortification. Vitamin A deficiency affects millions of children worldwide; therefore, increasing the content of vitamin A precursors in maize grains is of inte ...