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... The role of root interception, mass-flow and diffusion in supplying ions to soybeans growing in a leached subsoil was investigated. Root interception was the main mechanism for the supply of Cu, Al and Sr to the soybean roots. Mass-flow was the most important for B and diffusion was the most important for Mn, Fe and Zn. The most significant mechanisms will likely vary with the crop grown, climate ...
... Soybean varieties, representing 10 maturity groups (from North to South), differed significantly in their tolerance to acid Bladen soil (pH 4.4) which contains a high level of KCl-extractable Al. Previous evidence that Al toxicity is the primary growth-limiting factor in this soil, and the observation that varietal differences were minimized or eliminated by liming the soil to pH 5.5, led to the c ...
... Differential aluminum tolerance of ‘Perry’ and ‘Chief’ soybean varieties, determined previously from growth on acid Bladen soil, was confirmed in nutrient solutions containing Al as the known growth-limiting factor. Differences in Al tolerance between the two varieties were steadily increased as the Ca level of the nutrient solution was reduced from 50 to 8 to 2 ppm. Aluminum toxicity in soybeans ...
... Distribution of As with depth in soil was determined after 4 years of repeated applications of monosodium (MSMA), monoammonium (MAMA), and disodium methanearsonate (DSMA) to turf. Arsenic concentrations decreased with depth. Percentage recovery of applied As in the upper 30 cm of soil decreased with increasing application rates. Yields of cotton (Gossypium hirsutum L.), soybeans (Glycine max L. Me ...
... Short-term, split-root experiments were conducted in a growth chamber with ‘Lee’ soybeans (Glycine max). The upper portion of the root medium was a limed, fertilized sandy loam surface soil; the lower portion was a nutrient solution. Calcium concentration of 0.25 ppm was sufficient to obtain maximum growth rates in a nutrient solution at pH 5.6. More than 2.5 ppm of Ca was required at a solution p ...
... Seed yields of soybean (Glycine max L. Merr) mycorrhizal with Endogone were compared to yields from nonmycorrhizal plants in a P-deficient soil fertilized with various phosphate sources. Mycorrhizal outyielded nonmycorrhizal plants by 79, 530, 0, and 56% when fertilized with Al, Fe, rock, or monocalcium phosphate, respectively. Yields from mycorrhizal plants fertilized with Fe phosphate were ⅙ and ...
... Many varieties of soybean (Glycine max (L.) Merrill) are highly susceptible to the fungus Diaporthe sojae Lehman when grown in Delaware. It has been shown, in Iowa, that potassium levels in upper pods of soybean plants decreased markedly in late season. Because the upper pods frequently had the highest incidence of D. sojae in Delaware and the infection occurs in late season, the relationship of K ...
... The response of soybeans (Glycine max (L.) Merrill) to the direct and residual effect of liming in relation to soil acidity, Al and Mn toxicities and P availability was studied in five Oxisols of Southern Brazil under successive wheat (Triticum aestivum L.)-soybean cropping. Yield responses to liming were highly significant due to the high exchangeable Al and extractable Mn, the low pH, exchangeab ...
... Relatively little information is available on the effects of liming on roots as compared with tops. To study the effect of various Al saturations on the growth of tops, roots, nodules, and nutrient accumulation of soybeans (Glycine max L.), several rates of lime were added to an acid soil in a greenhouse experiment. Dry weight of tops increased as the Al saturation was decreased by liming. Total d ...
... A small amount of information is available on the effects of Al saturation on the nutrient concentration of different plant components. This manuscript presents data showing the former relative to the soybean [Glycine max (L.) Merr.]. To study the effects of varying exchangeable Al saturation on the concentration of P, Ca, Mg, and A1 in the tops, primary roots, lateral roots, and nodules of soybea ...
... Plant species and varieties within species differ in their response to mineral stresses and toxicities. To achieve maximum yields, the plant and the soil must be compatible. In the past, the soil has been changed to fit the plant. In this study, we consider changing the plant to fit the soil, recognizing that plant species and varieties within species have specific nutrient requirements. Ten soybe ...
... The objective of this field study was to determine the yield response of wheat (Triticum aestivum L.) to the direct and residual effects of liming in relation to variety, soil acidity, Al toxicity, P availability, and disease incidence in five Oxisols of Rio Grande do Sul, Brazil under successive wheat-soybean (Glycine max L. Merrill) cropping. This work was carried out under field conditions usin ...
... Growth of soybean [Glycine max (L.) Merr.] roots into acid subsoils and utilization of subsoil moisture is limited in many soils of the southeastern U. S. because of Al toxicity. Laboratory and greenhouse experiments were conducted to obtain information on tolerance of soybean cultivars which would be helpful in selection of cultivars best adapted to acid subsoils. Root elongation measurements wer ...
... Most studies of plant roots growing in soil involve destructive sampling or involve estimates of root growth only at a soil-viewing surface interface where growth conditions are different from those in bulk soil. This research tested the feasibility of using preferential attenuation of thermal neutrons by roots to overcome these two disadvantages of current methods. Radiographs were obtained of so ...
... Two biotites, a phlogopite, and a muscovite, ground to yield sandsize flakes, were used as sources of K for soybeans [Glycine max (L.) Merrill] grown in pot culture with and without vesicular-arbuscular mycorrhizal association. On the low-fertility soil used, effects of the mycorrhizae were most pronounced in terms of P uptake. Plants in noninoculated pots were stunted and P deficient; no K defici ...
... Laboratory techniques for screening soybean (Glycine max (L.) Merrill) genotypes for tolerance to Al would facilitate breeding programs for areas where AI toxicity limits soybean production. Genotypes from a broad-base soybean population were screened For Al tolerance based on root growth rate of 5-day-old seedlings grown in solutions containing 0.4 ppm Al and 10 ppm Ca (pH 4.6). The selections we ...
... A field survey of 220 soybean (Glycine max (L.) Merr.) plots was conducted over a 2-year period on mineral soils in 12 major soybean producing countries in the Coastal Plain of North Carolina. The objectives of this investigation were to study the effects of certain soil and management factors in soybean production and to develop statistical models for explaining variations in soybean grain yields ...
... High Al in acid subsoils of the Southeastern States often restricts root growth and moisture utilization. Liming subsoil is difficult and expensive. An alternative or substantial approach is to select or breed plant genotypes having greater tolerance to Al. The differential Al tolerances of soybean [ Glycine max(L.) Merr.] representing Maturity Groups V, VI, and VII were determined in a growth cha ...
... Soil fertility problems associated with soil acidity in the humid tropics were identified by observing variations of some growth-controlling factors along response curves. Data for these curves were generated by liming an Oxisol and an Ultisol of Hawaii so that pH increased progressively, from <5 to > 7. Legumes were grown across the pH gradients. Liming the Oxisol depressed Al concentration in th ...
... By-product gypsum has been accumulating in large mounds in southern Louisiana for over 25 yr and little has been utilized. The objective of this study was to determine if large applications of byproduct gypsum could be used to alleviate soil acidity. A column leaching experiment was conducted using an acid subsoil amended with 5.0 gypsum equivalents of phosphogypsum, hydrofluorogypsum, or pure gyp ...
... Organic matter is believed to be able to reduce Al toxicity in Ultisols. Since little information is available to substantiate this hypothesis, a greenhouse experiment was conducted to compare the effects of organic matter and lime on soybean [Glycine max (L.) Merr.] plants grown under increasing Al stress in a Bradson (Typic Hapludults) soil. Treatments, replicated three times, were 0, 25, 50, an ...
... Total aluminum (Al) in solution often comprises both monomeric and polymeric Al species. Concentrations of total and monomeric Al and Σ activities of monomeric Al species (Σ aAₗ ₘₒₙₒ) were determined in nutrient solutions at 40 µM Al with varying P/Al molar ratios (0, 0.02, 0.2, 2.0 and 4.0) and calcium (Ca) concentrations (0.5, 1.5, 5.0, 15 mM). Effects of these treatments on root elongation of s ...
... Aluminum species composition in solution is largely dependent on solution pH. Effects of Al on root length of soybean [Glycine max (L.) Merr.] were studied in solution culture using an incomplete factorial design comprising varying pH levels of 4.0, 4.2, 4.4, 4.6, 4.8, and 5.0 and added Al concentrations of 10, 15, 20, 25, 30 and 40 µM. These treatments caused calculated sum of activities of monom ...
... Growth of a legume in acid soils can be limited by the effects of infertility factors on the growth of the host plant per se or on nodulation and nodule function. Effects of varying solution pH (4.S, 5.0, 5.5) and external calcium (Ca) concentration (0.05, 0.5, 2.5 mM) on growth and nodulation of soybean [Glycine max (L.) Merr. cv. Fitzroy] were investigated in a solution culture experiment. Total ...
... The development of extremely to strongly acid (pH 3.5–5.5) minespoil at a surface coal mine in Illinois resulted in unusually low field corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] grain yields, particularly in years of severe drought stress. Spoil acidity, measured in terms of pH, was evaluated as a factor contributing to or interacting with drought stress. Drought stress was most clea ...
... The effect of varying two parameters having contrasting effects has been largely overlooked when describing Al phytotoxicity. Consequently the effects of varying pH (4.2, 4.5, and 4.8), Al (0, 20, 40, 80, and 160 µM Al) and CaSO₄ (625, 1250, 2500, 5000, and 10 000 µM Ca) levels on root length of soybeans (Glycine max (L.) Merr.) were evaluated in a solution culture experiment. At a given pH and Al ...
... A nutrient solution study was conducted to evaluate the effects of varying pH (4.2, 4.5, and 4.8), Al (0, 20, 40, and 80 µM Al) and F⁻ (0, 10, 20, and 40 µM F⁻) on soybean [Glycine max (L.) Merr. cv. Lee] root growth. The ability of Al fractions measured by the modified aluminon and 8-hydroxyquinoline methods in predicting the phytotoxic component of Al was also investigated. In the presence of Al ...
... This study quantified soil chemical properties as affected by land clearing method and subsequent soil management. It was conducted on a Yurimaguas soil (fine-loamy, siliceous, isohyperthermic Typic Paleudults) covered by a 20-yr-old evergreen forest in the Amazon Basin of Peru. The experimental design, a split plot replicated three times, included six main plot treatments: (i) slash and burn clea ...
... Because previous liming experiments in northern Alabama showed that critical soil pH for soybean [Glycine max (L.) Merr.] varied widely among sites, an on-farm research program was initiated to delineate the separate effects of likely acid-soil infertility factors in the area. Factorial treatments at 15 test sites included lime and Mo at rates of 0 and 4500 kg ha⁻¹ and 0 and 100 g ha⁻¹, respective ...
... The alleviation of Al toxicity by CaSO₄ is partly due to an increase in formation of less phytotoxic AlSO⁺₄ species. Since ion-pair formation is dependent on the solution pH, the magnitude of alleviation of Al toxicity by CaSO₄ may be influenced by pH. In the present study, the alleviation of Al toxicity (80 µM) to soybean [Glycine max (L.) Me root growth by CaSO₄ (625–10 000 µM) was investigated ...
... The use of gypsum or phosphogypsum (PG) as an ameliorant of subsoil acidity has been reported. However, the role of these amendments in aluminum (Al) speciation and solubility is not clearly understood. We studied the root growth of soybean [Glycine max (L.) Merr.] cv. Lee in dilute nutrient solution containing 2.0 g L PG without the addition of Al or in solutions containing 40 μM Al without any a ...
Glycine max; drought; water stress; aluminum; nutrient excess; subsoil; leaf water potential; transpiration; crop yield; greenhouse experimentation; stress response
Abstract:
... Drought and subsoil A1 are major concerns which face soybean [Glycine max (L.) Merr.] producers in the southeastern USA. However, the interaction of these two factors is not clearly understood. This study was designed to characterize the combined effect of these two stress factors on soybean water status. A greenhouse experiment was conducted, with two levels of subsoil A1 saturation (6 and 71%), ...
Glycine max; drought; water stress; aluminum; subsoil; toxic substances; genotype-environment interaction; introduced species; transpiration; dry matter accumulation; water content; genotype; water potential; stress response; North Carolina
Abstract:
... Soybean [Glycine max (L.) Merr.] producers in the southeastern USA are often confronted with the problems of drought and toxic levels of subsoil Al. Our objective in this study was to determine the reaction of diverse soybean genotypes to combined effects of these two stress factors. Greenhouse studies were initiated to examine the problem. Two levels of Al saturation in a subsoil layer (6 and 71% ...
... In a previous study we demonstrated the phytotoxicity of polynuclear hydroxy-Al, a finding that disagrees with a number of existing reports. The objectives of this study were to examine how toxicity of polynuclear Al might vary with experimental conditions or with choice of test plants, and to identify and characterize the toxic polynuclear species. Wheat (Triticum aestivum L. cv. Tyler or Seneca) ...
... Breeding for Al tolerance in soybean [Glycine max (L.) Merr.], has been hindered by confusion regarding screening methodology. This study was undertaken to determine the relationship between greenhouse and solution culture screening methods, using 12 diverse soybean genotypes. Greenhouse media consisted of a Goldsboro sandy loam (fine-loamy, siliceous, thermic Aquic Paledult) at 19% Al saturation ...
Glycine max; plant breeding; aluminum; metal tolerance; phytotoxicity; seedlings; roots; soil; electrons; hydrogen ions; selection criteria; line differences; genotype; thickness
Abstract:
... Soybean (Glycine max [L.] Merr.) roots are susceptible to A1 toxicity. This study tested the hypothesis that seedling characteristics could serve as selection criteria for plant tolerance to A1. Phenotypic-recurrent, divergent selection programs for seedling tolerance to A1 were completed within two broad-base soybean populations based on a laboratory screen. Genotypes differing in seedling tolera ...
... A greenhouse study was conducted to investigate the growth (dry matter yield) of selected legume cover crops; phytoaccumulation of metals such as Zn, Mn, Pb, Cu, Ni, and Al; and extractability of heavy metals from three different Alabama acid mine spoils. The spoils were amended based on soil test recommended levels of N, P, K, Ca, and Mg prior to plant growth. Metals were extracted by three extra ...
... Acidity constraints in Oxisols of the Amazon may entail both Al toxicity and Ca deficiency. A field study was conducted in a Xanthic Hapludox near Manaus, Brazil, to evaluate lime and Ca requirements for corn (Zea mays L.), cowpea (Vigna unguiculata L.), soybean [Glycine max (L.) Merr.], and peanut (Arachis hypogea L.) during five consecutive years. Calcitic lime was applied once at rates of 0, 0. ...
... Surface and subsurface acidity are of agronomic concern in the southeastern USA. The limits to crop production imposed by subsoil acidity need to be further defined for this region. Gypsum is being applied to ameliorate subsoil acidity and to overcome the problem of very slow lime movement from surface lime applications. Reduced crude conversion spent lime (RCCSL) containing anhydrite (CaSO₄) was ...
Glycine max; germplasm; acid soils; aluminum; metal tolerance; shoots; roots; dry matter accumulation; genotype-environment interaction; ozone; air pollution; genotype; Russia
Abstract:
... Nineteen soybean genotypes (ten from the former USSR, two from Brazil and seven from USA) were tested for aluminum (Al) tolerance by growing them for 21 days in greenhouse pots of acid, Al-toxic, unlimed Tatum (Typic Hapludult) subsoil at pH 4.0 and in limed subsoil at pH 5.1. Aluminum tolerance ranking depended upon the plant traits used in the screening process. Based on absolute dry shoot weigh ...
... Low activities of the monomeric aluminium (Al) species, Al³⁺, Al(OH)₂⁺ and Al(OH)²⁺ in solution reduce root growth and root hair development in soybean [Glycine max (L.) Merr.]. Calcium (Ca) ameliorates to a certain extent the toxic effects of Al on root growth, but the interactive effects of Ca and Al on root hair development are not known. In the absence of added Al in solution culture, Ca conce ...
... Acid soil [aluminum (Al)], toxicity and ozone toxicity are potential yield-limiting factors for soybeans [Glycine max (L.) Merr.]. Previous studies in our laboratory suggested that tolerance to these two stress factors coincided in some soybean cultivars. The objective of the current study was to determine the relationship between acid soil (Al) tolerance and ozone (O3) tolerance in a range of soy ...
... Aluminum is toxic to both plants and animals. Root growth and pollen-tube extension are inhibited after aluminum stress in acidic environments. Incubation of cultured neurons with aluminum results in the formation of neurofibrillar tangles reminiscent of the neural pathology observed in Alzheimer's disease. The present communication demonstrate that aluminum induced a rapid and dramatic increase i ...
Glycine max; diallel analysis; nutrient uptake; general combining ability; specific combining ability; tropics; heritability; aluminum; metal ions; metal tolerance; inheritance (genetics); Brazil
Abstract:
... The Brazilian tropical adapted soybeans contains, in addition to superior morphological characters, genetic factors for tolerance to cultivation in acidic, mineral-stressed soils. However, the selection process for these hindrances has been empirical, and information on the genetics of mineral element uptake by the plant is necessary. The objective of this investigation was to identify the mode of ...
Glycine max; aluminum; metal ions; phytotoxicity; ion transport; nutrient transport; nitrates; nutrient uptake; roots; dry matter accumulation; acidity
Abstract:
... A study was conducted to examine the hypothesis that the effects of external Al on NO3(-) uptake and assimilation depend upon the concentration of Al present. Young soybean seedlings [Clycine max (L.) Merrill, cv. Essex], growing under moderate acidity stress at pH 4.2, were exposed to a range of (Al3+) in solution for 3 d, and to labelled 99 atom % 15NO3(-) during the final hour of Al exposure. U ...
... Soil fumigation is used to eliminate plant pathogens and weeds in commercial practice and to eliminate organisms in controlled experiments, e.g., vesicular arbuscular mycorrhizal fungi (VAMF). This study was conducted to study the effect of methyl bromide fumigation on plant accumulation of 14 elements and assess plant colonization by VAMF after fumigation. Field experiments were conducted near Me ...
Glycine max; diallel analysis; metal tolerance; aluminum; general combining ability; specific combining ability; roots; field experimentation; hydroponics; mineral content; leaves; heritability; genotype-environment interaction; inheritance (genetics); chemical constituents of plants
Abstract:
... The soybean is a major crop in the agricultural systems of the Brazilian Cerrados (Savannahs) whose soils are acidic, devoid of nutrients and need to be amended before they are cultivated. However, below the ploughed layer there is a scarcity of nutrients and toxic aluminium (Al). These limit root growth, subsequently causing nutritional imbalance and drought stress. Our aim in the investigation d ...
... Al localization was compared in three developmental regions of primary root of an Al-sensitive soybean (Glycine max) genotype using secondary ion mass spectrometry. In cryosections obtained after a 4-h exposure to 38 micromolar [Al3+], Al had penetrated across the root and into the stele in all three regions. Although the greatest localized Al concentration was consistently at the root periphery, ...
... Soil acidity and aluminum toxicity are serious environmental problems often found in humid temperate and tropical regions or in areas with acid rain. Iron oxidation in soils can also cause high concentrations of H, which, in turn, causes an increase of Al in the soil solution. To examine this problem, a study was undertaken to discover the cause of crop damage in crops planted over buried anode ba ...
... Alleviation by calcium (Ca) of inhibition of soybean [Glycine max (L.) Merr. cv. 'Ransom') root elongation by hydrogen (H) and aluminum (Al) was evaluated in a vertical split-root system. Roots extending from a limed and fertilized soil compartment grew for 12 days into a subsurface compartment containing nutrient solution with treatments consisting of factorial combinations of either pH (4.0, 4.6 ...
... AIuminum toxicity restricts soybean [Glycine max (L.) Merr.] yield included many growing areas. When correction of toxicity by management is impractical, an economically sound alternative is to develop AI tolerant cultivars. Heritability (h²) estimates for AI tolerance in hydroponics would aid in the efficient design of selection programs for cultivar development. Our objectives were to determine ...
... Although crop seedling root systems are made up of several types of root (e.g., tap, basal, and lateral roots) there has been little attempt to determine their respective sensitivity to abiotic stresses. Most acidic agricultural fields are treated so that they are only slightly or non-Al-toxic in the topsoil with Al-toxicity in the subsoil—effectively stratifying the soil profile relative to Al-to ...
... Shallow rooting and susceptibility to drought are believed to be caused, at least in part, by strongly acidic (pH < 5.5, 1:1 soil-water), aluminum (Al)-toxic subsoils. However, this hypothesis has not been clearly confirmed under field conditions. The Al toxicity hypothesis was tested on a map unit of Matawan-Hammonton loam (0-2% slope) on unlimed and limed field plots (pH range 5.1 to 5.8) at Bel ...
... Soil pH is an important soil chemical property of acid soils that profoundly affects the growth and nutrient uptake by crops. Five greenhouse experiments were conducted to evaluate responses of common bean (Phaseolus vulgaris L.), lowland rice (Oryza sativa L.), corn (Zea mays L.), soybean (Glycine max L. Merr.). and wheat (Triticum aestivum L.) to mean soil pH values of 4.9, 5.9, 6.4, 6.7, and 7 ...
... Soybean [Glycine max (L.) Merr.] plant introduction PI 416937 (PI4) has an extensive fibrous-like root system that contributes to enhanced drought and Al tolerance. The root system of PI4 appears to be more highly nodulated than standard southern U.S. cultivars, and thus has potential for enhanced N₂ fixation. Genetic transfer of PI4 root system to soybean cultivars may lead to increased seed N at ...
... Computer-based technologies are becoming increasingly important in translating the wealth of agricultural research information into forms that can be used by extension workers. The Acidity Decision Support System (ADSS) is a computer-based expert system that diagnoses and corrects Al-toxicity problems on highly-weathered mineral soils. The objectives of this work were to use published data to eval ...
... Acid soils with high levels of Al impede root growth, causing increased crop sensitivity to drought and decreased nutrient acquisition. Development of Al-tolerant cultivars may be a cost effective response to the problem. In previous investigations, we identified an Al-tolerant soybean [Glycine max (L.) Merr.] plant introduction from Japan (PI 416937), and subsequently determined the heritability ...
... Subsoil A1 can restrict deep root growth which, under dryland conditions, can cause lower yields during periods of water deficit. Soils that have high levels of subsoil Al must be identified so that appropriate management strategies, such as selection of deep rooting, Al tolerant cultivars or chemical amelioration of the subsoil, can be implemented. Thirteen soils representing three landscape posi ...
... Aluminum-tolerant germplasm is needed to overcome subsurface acidity constraints to root growth and plant access to water and nutrients. Root elongation of four soybean [Glycine max (L.) Merr.] genotypes exposed to varying concentrations of Al, H, and Ca were compared in two experiments using a vertically split root system. Roots extending from a limed surface soil compartment grew for 12 d into a ...
... Variations in genotype rankings among screenings for Al tolerance in hydroponics may be related to differences in the composition of the solutions. In the present study, we investigated the involvement of Mg ions in modifying Al rhizotoxicity in soybeans. Root elongation was strongly inhibited by Al in a simple, 800 micromolars CaSO4 solution, but elongation increased noticeably when the solutions ...
... Flooding is a major problem that reduces soybean [Glycine max (L.) Merr.] growth and grain yield in many areas of the USA and the world. Our objective was to identify the plant and soil characteristics associated with different flooding durations in six fields in central Ohio. The soybean plants were at the V2 and V3 stages when rainfall-induced flooding occurred. The outer perimeters of the flood ...
... Screening methodology remains a practical barrier in the breeding of Al-tolerant soybean [Glycine max (L.) Merr.]. Our objectives were to (i) develop a repeatable sand-media culture method for Al tolerance screening of plants, (ii) compare Al response of genotypes in sand culture to a standard hydroponics-based seedling culture, and (iii) establish a practical guide for the use of hydroponics and ...
... Superior effectiveness of Mg over Ca in alleviating Al rhizotoxicity cannot be accounted for by predicted changes in plasma membrane Al3+ activity. The influence of Ca and Mg on the production and secretion of citrate and malate, and on Al accumulation by roots was investigated with soybean genotypes Young and PI 416937 which differ in Al tolerance. In the presence of a solution Al3+ activity of 4 ...
... The mechanistic basis for cation amelioration of Al rhizotoxicity in soybean was investigated through a series of studies comparing protective effects of Ca and Mg against Al inhibition of root elongation in a background 0.8 mM CaSO4 solution (pH 4.3). A modified Gouy-Chapman-Stern model was used to evaluate the effect of cations on electrical potential and Al3+ activity at root plasma membrane su ...
... Because plant absorption of P depends on the desorption of P from soil, understanding P desorption from soils may improve the precision of P diagnosis and fertilization recommendations. Many soils with high P retention due to high levels of Fe and Al are also highly aggregated. Extractable P is sometimes higher on larger size aggregates, which will probably result in increased P release from aggre ...
... Spodic horizon in Spodosols often restricts growth of plant roots. Generally the Spodic horizon is acidic with high levels of aluminum (Al). In this study soil solution Al was characterized in the Spodic horizon and the respective surface horizon of three Spodosols in the major citrus production region in Florida. The soil pH ranged from 4.92 to 5.14, and 4.82 to 5.50 in the Spodic and top soil sa ...
Glycine max; aluminum; apoplast; magnesium chloride; polyacrylamide gel electrophoresis; root tips; seedlings; soy protein; soybeans; two-dimensional gel electrophoresis
Abstract:
... Aluminium effect on the mobility of apoplast protein in root tips was studied. Two-day seedlings of soybean (Glycine max. (L.) Merr. cv. Tsurunoko) were treated with 50 μM AlCl₃ for 2 h. Using infiltration method, the apoplast protein in root tips was extracted with 20 or 100 mM MgCl₂. When 20 mM MgCl₂ was used to collect weakly bound protein to apoplast, the amount of protein extracted was reduce ...
... The role of calcium (Ca) and the effect of different concentrations of aluminum (Al) on contents of Ca, chlorophyll (a + b), carotenoids, chlorophyll-protein complexes in soybean (Glycine max Merr.) leaves were observed. The Ca content in leaves decreases with the increase of Al concentrations in the solution, i.e., with the decrease of a Ca : Al molar ratio. Furthermore, regardless of Ca presence ...
... The aluminum (Al)-induced secretion of citrate has been regarded as an important mechanism for Al resistance in soybean (Glycine max). However, the mechanism of how Al induces citrate secretion remains unclear. In this study, we investigated the regulatory role of plasma membrane H⁺-ATPase on the Al-induced secretion of citrate from soybean roots. Experiments performed with plants grown in full nu ...
... Nitrogen fixation and assimilation in nodules and roots were studied in soybean (Glycine max L.) exposed to different levels of aluminium (Al) stress (0, 50, 200 and 500 μM). Al at 500 μM induced oxidative stress, which became evident from an increase in lipid peroxidation accompanied by a concomitant decline in antioxidant enzyme activities and leghaemoglobin breakdown. Consequently, there was al ...
... Aluminum (Al) toxicity and phosphorus (P) deficiency often coexist in acid soils that severely limit crop growth and production, including soybean (Glycine max). Understanding the physiological mechanisms relating to plant Al and P interactions should help facilitate the development of more Al-tolerant and/or P-efficient crops. In this study, both homogeneous and heterogeneous nutrient solution ex ...
... Hydroponic studies with soybean (Glycine max [L.] Merr.) have shown that μmol L⁻¹ additions of Mg²⁺ were as effective in ameliorating Al rhizotoxicity as additions of Ca²⁺in the mmol L⁻¹ concentration range. The objectives of this study were to assess the ameliorative effects of Mg on soybean root growth in acidic subsoils and to relate the soil solution ionic compositions to soybean root growth. ...
... To investigate the genetic mechanism of Al-tolerance in soybean, a recombinant inbred line population (RIL) with 184 F₂:₇:₁₁ lines derived from the cross of Kefeng No.1 x Nannong 1138-2 (Al-tolerant x Al-sensitive) were tested in pot experiment with sand culture medium in net room in Nanjing. Four traits, i.e. plant height, number of leaves, shoot dry weight and root dry weight at seedling stage, ...
... A 3-year field trial examined in a long-term no-till system the effects of surface-applied lime and cover black oat (Avena strigosa Schreb) residues on soil chemical attributes, root growth and grain yield of corn (Zea mays L.) and soybean (Glycine max L. Merrill) on a loamy, kaolinitic, thermic Typic Hapludox in Paraná State, Brazil. The treatments consisted of dolomitic lime broadcast on the soi ...
... Seedlings of two soybean genotypes, BX10 [aluminum (Al)-tolerant] and BD2 (Al-sensitive), were treated with Al to evaluate the relative root growth (RRG), callose content, Al-sensitive zone, lipid peroxidation, and the anti-oxidative enzyme activities by histochemical and biochemical assays. Under Al toxicity, the RRG reduction of BD2 was more significant than that of BX10, while callose content d ...
... Aluminum (Al) and manganese (Mn) toxicity are two main constraints to crop production in some acid soils. In the present study, hydroponic experiments were conducted to investigate the phytoxicity caused by the metals Al and Mn in the Al-sensitive soybean (Glycine max) cultivar Jilin62. Results showed that more than 80% of the total cellular Al accumulated in the cell walls of the root tissue. Exc ...
... To further understand the process of Al-induced citrate secretion from soybean roots, the effect of protein synthesis inhibitor, anion channel blockers, and citrate carrier inhibitors on Al-induced citrate exudation was investigated in Al-resistant soybean cultivar PI 416937. Citrate exudation from roots increased with the increase of Al concentration from 10 to 50 μM and initiated after 4 h of Al ...
... Additions of micromolar concentrations of magnesium (Mg) to hydroponics enhance aluminum (Al) tolerance of soybean by increasing citrate secretion from roots and external complexation of toxic Al species. The objective of this study was to assess the ameliorative effect of Mg additions on soybean root elongation into mineralogically different acid soils. Roots of soybean seedlings grew for 28 days ...
... The concentration of soluble aluminium (Al) in the soil solution increases at low pH and the prevalence of toxic Al³⁺ cations represent the main factor limiting plant growth on acid soils. Citrate secretion from roots is an important Al-tolerance mechanism in many species including soybean. We isolated mitochondria from the roots of an Al-resistant soybean (Glycine max L.) cv. Jiyu 70 to investiga ...
... Upland agriculture in Indonesia mainly relies on Ultisols and Oxisols, which have serious problems resulting from severe erosion and low organic-matter content. The objectives of the study were (i) to assess the effect of long-term rehabilitation techniques on soil organic carbon (SOC) and maize yields of a desurfaced Ultisol and (ii) to assess the effect of short-term rehabilitation techniques on ...
... Abscisic acid (ABA) plays an important role in mediating some biotic and abiotic stresses. In the present study, to better understand the relationship of ABA production and Aluminum (Al)-resistance in plants, Al-resistance genotype (Jiyu70) of soybean was adopted to investigate the accumulation and transport of ABA in plants exposed to Al. Results showed that exogenous application of ABA and ABA s ...
... Aluminum (Al) is not the necessary nutrient but the most abundant metal in the earth’s crust. Isozymes of the seedlings of two soybean varieties (Zhechun No. 3 and Huachun No. 18) and their response to different Al treatments had been analyzed. The study showed that the responses to Al toxicity of different isozymes were quite different. Among these four isozymes, esterase (EST) isozym ...
... Aluminum (Al) is a harmful element that rapidly inhibits the elongation of plant roots in acidic soils. The release of organic anions explains Al resistance in annual crops, but the mechanisms that are responsible for superior Al resistance in some woody plants remain unclear. We examined cell properties at the surface layer of the root apex in the camphor tree (Cinnamomum camphora) to understand ...
... Gypsum has been used in tropical and subtropical agriculture when subsoil acidity is an important yield-limiting factor. However, the conditions that promote increased crop yield as a result of gypsum addition in no-till (NT) systems still remain unclear. A field trial examined the effects of newly and previously surface-applied gypsum in a long-term NT system on the soil chemical properties and n ...