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1. Biochemical, transcriptomic and metabolomic responses to total dissolved gas supersaturation and their underlying molecular mechanisms in Yangtze sturgeon (Acipenser dabryanus)

Author:
Yeyu Chen; Xiaoyun Wu; Xiaoqing Liu; Jiansheng Lai; Ya Liu; Mingjiang Song; Feiyang Li; Quan Gong
Source:
Environmental research 2023 v.216 pp. 114457
ISSN:
0013-9351
Subject:
Acipenser dabryanus; amino acid metabolism; catalase; cell membranes; dissolved gases; energy; fish; flagship species; glycerophospholipids; heat stress; histopathology; liver; metabolites; metabolomics; research; superoxide dismutase; transcriptomics; ubiquitin; water power; Yangtze River
Abstract:
... With the rapid development of hydropower facility construction, the total dissolved gas (TDG) generated by dam discharge is seriously threatening the survival of fish and has become an ecological environmental issue of global concern. However, how TDG affects fish physiology and the underlying molecular mechanism remain poorly known. In this study, Acipenser dabryanus, an ancient living fossil tha ...
DOI:
10.1016/j.envres.2022.114457

2. Metabolomics reveals the effect of hypobaric treatment on energy metabolism in vibration-injured ‘Huangguan’ pears

Author:
Fangxu Xu; Guode Li
Source:
Food chemistry 2023 v.400 pp. 134057
ISSN:
0308-8146
Subject:
adenosine triphosphate; amino acid metabolism; biosynthesis; energy; energy metabolism; food chemistry; fruit quality; fruits; mechanical damage; metabolomics; secondary metabolites; transportation; vibration
Abstract:
... Mechanical damage caused by vibration during transportation can destroy organization structure and reduce the fruit quality. The objective was to reveal the mechanism of hypobaric treatment on energy metabolism in vibration-injured 'Huangguan' pears based on metabolomics. Results showed that hypobaric treatment delayed the decline of adenosine triphosphate (ATP) content, energy charge (EC), H⁺-ATP ...
DOI:
10.1016/j.foodchem.2022.134057

3. Cholic acid supplementation accelerates the progression of nonalcoholic fatty liver disease to the procarcinogenic state in mice fed a high-fat and high-cholesterol diet

Author:
Hee Jeong Chun; Yeon Joo Shim; Young Hye Kwon
Source:
Journal of nutritional biochemistry 2022 v.100 pp. 108869
ISSN:
0955-2863
Subject:
amino acid metabolism; apoptosis; biosynthesis; carcinogenicity; cholesterol; cholic acid; diethylnitrosamine; drugs; energy; fatty liver; gene expression; gene expression regulation; hepatoma; high fat diet; histology; inflammation; microarray technology; mitochondria; necrosis; oxidative phosphorylation; steroid metabolism; triacylglycerols
Abstract:
... Nonalcoholic fatty liver disease (NAFLD) is one of the major causes of hepatocellular carcinoma (HCC). Although the intracellular cholesterol accumulation has been demonstrated to regulate the gene expression responsible for steatohepatitis, the role played by cholesterol in the development of NAFLD-associated HCC has not been fully elucidated. In this study, using microarray analysis, we investig ...
DOI:
10.1016/j.jnutbio.2021.108869

4. Comprehensive analysis of Sparassis crispa polysaccharide characteristics during the in vitro digestion and fermentation model

Author:
Wenyi Zhang; Bin Hu; Chang Liu; Hanyi Hua; Yahui Guo; Yuliang Cheng; Weirong Yao; He Qian
Source:
Food research international 2022 v.154 pp. 111005
ISSN:
0963-9969
Subject:
Dialister; Escherichia; Megamonas; Megasphaera; Prevotella; Shigella; Sparassis crispa; acetates; amino acid metabolism; butyrates; digestion; digestive tract; energy; fermentation; food research; humans; intestinal microorganisms; medicine; models; mushrooms; polysaccharides; prebiotics; prediction; propionic acid
Abstract:
... Sparassis crispa (S.crispa), an edible mushroom, is widely used as a natural medicine due to its excellent pharmacological activities, including antitumor, anti-angiogenic, and immunomodulatory activities. In the current study, the digestion and fermentation characteristics of an S.crispa polysaccharide (SCP-1) were investigated by the in vitro simulated models. Our results revealed that SCP-1 was ...
DOI:
10.1016/j.foodres.2022.111005

5. Crosstalk between Hypoxia and Extracellular Matrix in the Tumor Microenvironment in Breast Cancer

Author:
Yasmin Dekker; Sylvia E. Le Dévédec; Erik H. J. Danen; Qiuyu Liu
Source:
Genes 2022 v.13 no.9 pp. -
ISSN:
2073-4425
Subject:
amino acid metabolism; amino acids; breast neoplasms; energy; extracellular matrix; glucose; glycolysis; hypoxia; lipids; mechanotransduction; metastasis; neoplasm progression; pH
Abstract:
... Even though breast cancer is the most diagnosed cancer among women, treatments are not always successful in preventing its progression. Recent studies suggest that hypoxia and the extracellular matrix (ECM) are important in altering cell metabolism and tumor metastasis. Therefore, the aim of this review is to study the crosstalk between hypoxia and the ECM and to assess their impact on breast canc ...
DOI:
10.3390/genes13091585

6. Dietary guanidineacetic acid supplementation ameliorated meat quality and regulated muscle metabolism of broilers subjected to pre-slaughter transport stress by metabolomics analysis

Author:
Bolin Zhang; Ning Liu; Kui Kang; Renbo Zhang; Meilin Hao; Peiyong Song; Qingrong Wang; Yuxiao Xie; Chuntao Li
Source:
Poultry science 2022 v.101 no.4 pp. 101739
ISSN:
0032-5791
Subject:
adenosine diphosphate; adenosine monophosphate; adenosine triphosphate; alanine; amino acid metabolism; citrulline; creatine; cysteine; energy; energy metabolism; gas chromatography-mass spectrometry; gene expression; glutathione; high performance liquid chromatography; meat quality; metabolites; metabolome; metabolomics; methionine; muscles; oxidative stress; pectoralis muscle; phenylalanine; phosphocreatine; sarcosines; serine; slaughter; threonine; tryptophan; tyrosine
Abstract:
... Pre-slaughter transport stress could induce multiple comprehensive variations in physiological and metabolic parameters of broilers. However, the entire metabolomics of pre-slaughter transport stress and supplementation of exogenous energy regulatory substances on broilers is still poorly understood. The metabolome characteristics of broilers subjected to 3 h pre-slaughter transport stress combine ...
DOI:
10.1016/j.psj.2022.101739

7. Dietary supplementation of Synechococcus elongatus PCC 7942 expressing a heterologous β-glucosidase on the expression of genes related to digestion, immune system, and antioxidant defenses of the shrimp Litopenaeus vannamei

Author:
Luiza Medeiros; Raíza Azevedo; Jade Riet; Bruna Nornberg; Arthur Cardoso; Victor Torres Rosas; Marcelo Borges Tesser; Virgínia F. Pedrosa; Luis A. Romano; Wilson Wasielesky Jr; Luis Fernando Marins
Source:
Journal of applied phycology 2022 v.34 no.4 pp. 2089-2098
ISSN:
0921-8971
Subject:
Litopenaeus vannamei; Synechococcus elongatus; Synechococcus sp. PCC 7942; absorption; algology; amino acid metabolism; antioxidants; carbohydrate metabolism; dietary supplements; digestion; energy; environmental impact; genetically modified organisms; growth performance; hepatopancreas; histology; immune system; lipids; muscle tissues; muscles; phosphorus; proximate composition; shrimp
Abstract:
... Cyanobacteria are a rich source of nutrients in addition to producing bioactive compounds capable of stimulating the immune system of hosts. Also, they can be manipulated with relative ease to produce heterologous proteins. In the present study, a strain of Synechococcus elongatus (PCC7942) was manipulated to produce a prokaryotic β-glucosidase. This strain was added to the diet of Pacific white s ...
DOI:
10.1007/s10811-022-02748-5

8. Effect of Chinese Herbs on Serum Biochemical Parameters, Immunity Indices, Antioxidant Capacity and Metabolomics in Early Weaned Yak Calves

Author:
Cuixia Jiang; Quanmin Dong; Xiaoping Xin; Abraham Allan Degen; Luming Ding
Source:
Animals 2022 v.12 no.17 pp. -
ISSN:
2076-2615
Subject:
Angelica sinensis; Codonopsis pilosula; Glycyrrhiza uralensis; albumins; amino acid metabolism; antioxidant activity; blood serum; carbon; cholesterol; energy; glutathione peroxidase; immune response; malondialdehyde; metabolomics; nitrogen metabolism; yaks
Abstract:
... Chinese traditional herbs are used widely as feed supplements to improve the immune response and antioxidant capacity of livestock. Twenty early-weaned 4-month-old yak calves (72.3 ± 3.65 kg) were divided randomly into four groups (n = 5 per group); three groups were provided with supplementary 80 mL/kg DMI of the root water extracts of either Angelica sinensis, Codonopsis pilosula or Glycyrrhiza ...
DOI:
10.3390/ani12172228

9. Field mold stress induced catabolism of storage reserves in soybean seed and the resulting deterioration of seed quality in the field

Author:
DENG Jun-cai; LI Xiao-man; XIAO Xin-li; WU Hai-jun; YANG Cai-qiong; LONG Xi-yang; ZHANG Qi-hui; IQBAL Nasir; WANG Xiao-chun; YONG Tai-wen; DU Jun-bo; YANG Feng; LIU Wei-guo; ZHANG Jing; WU Xiao-ling; WU Yu-shan; YANG Wen-yu; LIU Jiang
Source:
Journal of integrative agriculture 2022 v.21 no.2 pp. 336-350
ISSN:
2095-3119
Subject:
agriculture; amino acid metabolism; biosynthesis; catabolism; energy; fatty acids; glycolysis; greenhouses; isoflavones; metabolome; polysaccharides; rain; seed quality; seed yield; soybeans; sugars; transcription (genetics); transcriptome
Abstract:
... Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold (FM) stress. If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality. To investigate the responses of soybean seed against FM stress and identify the underlying biochemical pathways involved, a greenhouse was equipped with an art ...
DOI:
10.1016/S2095-3119(20)63594-8

10. Growth performance and metabolic responses of Nile tilapia fed diets with different protein to energy ratios

Author:
H. Peres; J.M.A. Freitas; P.L.P. Carvalho; W.M. Furuya; M.M.P. Satori; A. Oliva-Teles; L.E. Pezzato; M.M. Barros
Source:
Aquaculture 2022 v.547 pp. 737493
ISSN:
0044-8486
Subject:
Oreochromis niloticus; amino acid composition; amino acid metabolism; aquaculture; biochemical pathways; body weight; crude protein; digestibility; digestible energy; digestible protein; energy; feed conversion; feed intake; fillets; gluconeogenesis; glycolysis; growth performance; lipid content; lipids; lipogenesis; muscles; nitrogen retention; weight gain
Abstract:
... The effect of dietary digestible protein to digestible energy (DP/DE) ratio on the growth performance and metabolic responses of Nile tilapia juveniles was evaluated. Five experimental diets were formulated with increasing DP/DE ratio (13.2, 15.5, 18.4, 20.0, and 22.6 mg kJ⁻¹) and fed to eight replicates of Nile tilapia (initial body weight of 9.3 g) for 110 days. The apparent digestibility coeffi ...
DOI:
10.1016/j.aquaculture.2021.737493

11. Hallmarks of Metabolic Reprogramming and Their Role in Viral Pathogenesis

Author:
Charles N. S. Allen; Sterling P. Arjona; Maryline Santerre; Bassel E. Sawaya
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
amino acid metabolism; antiviral agents; biomass; biosynthesis; energy; glycolysis; immune system; lactic acid; lipid metabolism; mitochondria; pathogenesis; pentose phosphate cycle; therapeutics; viral genome
Abstract:
... Metabolic reprogramming is a hallmark of cancer and has proven to be critical in viral infections. Metabolic reprogramming provides the cell with energy and biomass for large-scale biosynthesis. Based on studies of the cellular changes that contribute to metabolic reprogramming, seven main hallmarks can be identified: (1) increased glycolysis and lactic acid, (2) increased glutaminolysis, (3) incr ...
DOI:
10.3390/v14030602

12. Hepatic transcriptome profiling according to growth rate reveals acclimation in metabolic regulatory mechanisms to cyclic heat stress in broiler chickens

Author:
C. Lim; B. Lim; D.Y. Kil; J.M. Kim
Source:
Poultry science 2022 pp. 102167
ISSN:
0032-5791
Subject:
RNA; acclimation; amino acid metabolism; antioxidants; cell cycle checkpoints; climate change; energy; fatty acids; gene expression; gene expression regulation; genes; growth performance; heat shock proteins; heat stress; homeostasis; liver; poultry; protein synthesis; transcriptome
Abstract:
... Climate change has numerous effects on poultry that result in welfare concerns and economic losses in agricultural industries. However, the mechanisms underlying the acclimation to heat stress in poultry have not been comprehensively defined. Therefore, identifying associated patterns of gene regulation and understanding the molecular mechanisms of acclimation to a warmer environment will provide ...
DOI:
10.1016/j.psj.2022.102167

13. Insight into the differences in the toxicity mechanisms of dinotefuran enantiomers in zebrafish by UPLC-Q/TOF–MS

Author:
Xia Zhou; Ya Yang; Renyue Ming; Hong Chen; Deyu Hu; Ping Lu
Source:
Environmental science and pollution research 2022 v.29 no.47 pp. 70833-70841
ISSN:
0944-1344
Subject:
Danio rerio; Nilaparvata lugens; amino acid metabolism; arginine; biochemical pathways; biosynthesis; dinotefuran; discriminant analysis; enantiomers; enantioselectivity; energy; glycerophospholipids; lipid metabolism; metabolites; phenylalanine; pollution; principal component analysis; proline; research; risk; toxicity; tryptophan; tyrosine; vitamin A
Abstract:
... Dinotefuran is a chiral insecticide widely used to control Nilaparvata lugens in agriculture. However, little is known about the toxic effects of dinotefuran enantiomers on aquatic organisms. In this study, zebrafish were exposed to 1.00 and 10.00 mg/L dinotefuran enantiomers for 96 h, after which multivariate pattern recognition, metabolite identification, and pathway analysis were performed. Pri ...
DOI:
10.1007/s11356-022-20424-6

14. Integrated metabolomic and transcriptomic strategies to reveal adaptive mechanisms in castor plant during germination stage under alkali stress

Author:
Peilin Han; Shuxin Li; Kunshu Yao; Heyang Geng; Junyu Liu; Yingnan Wang; Jixiang Lin
Source:
Environmental and experimental botany 2022 v.203 pp. 105031
ISSN:
0098-8472
Subject:
Ricinus communis; alkalinity; amino acid metabolism; biosynthesis; carbohydrate metabolism; energy; fatty acid composition; gene expression; gene expression regulation; lipid metabolism; malondialdehyde; membrane fluidity; metabolites; metabolome; metabolomics; oils; oilseed crops; plant establishment; proline; salt stress; seed germination; soil remediation; transcriptome; transcriptomics; tricarboxylic acid cycle; unsaturated fatty acids; China
Abstract:
... Castor (Ricinus communis L.) is an important oilseed crop worldwide, and is also considered as one of the most promising plants for alkali-affected soil amelioration in the northeast of China. Seed germination plays a vital role in seedling establishment, and is always sensitive to alkali stress. However, little is known about the dynamic changes and regulatory mechanisms of gene expression and me ...
DOI:
10.1016/j.envexpbot.2022.105031

15. Integrated transcriptome and proteome analyses provide insight into abiotic stress crosstalks in bermudagrass

Author:
Zhengfu Fang; Chunling Liu; Tiantian Ye; Chao Huang; Zhewei Zhang; Yanping Wang; Zhulong Chan
Source:
Environmental and experimental botany 2022 v.199 pp. 104864
ISSN:
0098-8472
Subject:
Arabidopsis; Cynodon dactylon; RNA; amino acid metabolism; drought; electron transfer; energy; germination; glycolysis; nitrogen metabolism; photosynthesis; proteome; proteomics; salt stress; sequence analysis; stress response; stress tolerance; submergence; transcriptome; transcriptomics; turf grasses; unigenes; warm season
Abstract:
... Bermudagrass is a widely used warm-season turfgrass species with superior stress tolerance. Due to limited genome information, detailed stress response mechanisms of bermudagrass were not extensively characterized. In this study, we compared transcriptomic and proteomic level changes of bermudagrass after treatments with drought, salt and submergence. The results showed that 31.9–61.4% unigenes we ...
DOI:
10.1016/j.envexpbot.2022.104864

16. Kidney Bean Fermented Broth Alleviates Hyperlipidemic by Regulating Serum Metabolites and Gut Microbiota Composition

Author:
Weiqiao Pang; Di Wang; Zhaohang Zuo; Ying Wang; Wei Sun; Naidan Zhang; Dongjie Zhang
Source:
Nutrients 2022 v.14 no.15 pp. -
ISSN:
2072-6643
Subject:
amino acid metabolism; blood serum; energy; flavonoids; functional foods; glycerophospholipids; high fat diet; humans; hyperlipidemia; intestinal microorganisms; kidney beans; metabolites; metabolomics; models; polyphenols; taurine; tissue weight; weight gain
Abstract:
... Hyperlipidemia with fat accumulation and weight gain causes metabolic diseases and endangers human body health easily which is accompanied by metabolic abnormalities and intestinal flora disorders. In this study, the kidney bean fermented broth (KBF) was used in rats that were fed a high-fat diet to induce hyperlipidemia in order to subsequently analyse the serum metabolomics and gut microbiota mo ...
DOI:
10.3390/nu14153202

17. Metabolic Snapshot of Plasma Samples Reveals New Pathways Implicated in SARS-CoV-2 Pathogenesis

Author:
Oihane E. Albóniga; Daniel Jiménez; Matilde Sánchez-Conde; Pilar Vizcarra; Raquel Ron; Sabina Herrera; Javier Martínez-Sanz; Elena Moreno; Santiago Moreno; Coral Barbas; Sergio Serrano-Villar
Source:
Journal of proteome research 2022 v.21 no.3 pp. 623-634
ISSN:
1535-3907
Subject:
COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; amino acid metabolism; biomarkers; capillary electrophoresis; citric acid; citrulline; disease severity; energy; health services; histidine; humans; hypoxia; liver; metabolites; metabolomics; mitochondria; pathogenesis; phenylalanine; proteome; research; risk; spectrometers; thrombosis
Abstract:
... Despite the scientific and human efforts to understand COVID-19, there are questions still unanswered. Variations in the metabolic reaction to SARS-CoV-2 infection could explain the striking differences in the susceptibility to infection and the risk of severe disease. Here, we used untargeted metabolomics to examine novel metabolic pathways related to SARS-CoV-2 susceptibility and COVID-19 clinic ...
DOI:
10.1021/acs.jproteome.1c00786

18. Metabolic reprogramming and alteration of the redox state in hyper-productive MDCK cells for influenza a virus production

Author:
Qian Ye; Xuping Liu; Yuxiang Wan; Wen-Song Tan; Liang Zhao
Source:
Biologicals 2022 pp. -
ISSN:
1045-1056
Subject:
Influenza A virus; amino acid metabolism; amino acids; cell lines; dogs; energy; financial economics; glucose; influenza; influenza vaccines; kidneys; mitochondria; morbidity; mortality; phenotype; protein synthesis; public health; reactive oxygen species; tricarboxylic acid cycle
Abstract:
... Influenza is a global public health issue leading to widespread morbidity and mortality with devastating economic loss annually. Madin-Darby Canine Kidney (MDCK) cell line has been a major cell line for influenza vaccine applications. Though many details of the host metabolic responses upon influenza A virus (IAV) infection have been documented, little is known about the metabolic reprogramming fe ...
DOI:
10.1016/j.biologicals.2022.08.004

19. Oxidative stress, apoptosis, and transcriptional responses in Acropora microphthalma under simulated diving activities

Author:
Yu Zhang; Rou-Wen Chen; Xiangbo Liu; Ming Zhu; Zhuoran Li; Aimin Wang; Xiubao Li
Source:
Marine pollution bulletin 2022 v.183 pp. 114084
ISSN:
0025-326X
Subject:
Acropora; amino acid metabolism; apoptosis; autophagy; beta oxidation; biosynthesis; caspase-3; corals; energy; fatty acids; lipopolysaccharides; marine pollution; oxidative stress; photorespiration; transcription (genetics); transcriptome
Abstract:
... This study simulated the effects of diving activities on the physiology, enzymatic, and transcriptional responses of Acropora microphthalma. Touching had less impact on Fv/Fm, but a few zooxanthellae were decreased and minor MDA was elevated. Caspase 3 was activated to remove damaged cells, and SOD was increased to alleviate oxidative damage. Under double or triple diving stress, we observed mass ...
DOI:
10.1016/j.marpolbul.2022.114084

20. Physiological and proteomic analyses of Tunisian local grapevine (Vitis vinifera) cultivar Razegui in response to drought stress

Author:
Wassim Azri; Rahma Jardak; Pascal Cosette; Clément Guillou; Jawaher Riahi; Ahmed Mliki; Manuela Chaves
Source:
Functional plant biology 2022 v.49 no.1 pp. 25-39
ISSN:
1445-4408
Subject:
Calvin cycle; Vitis vinifera; acclimation; amino acid metabolism; climate change; cultivars; drought; drought tolerance; energy; new species; osmotolerance; photorespiration; protective effect; protein synthesis; proteomics; signal transduction; viticulture; water stress
Abstract:
... Drought is one of the major environmental constraints threatening viticulture worldwide. Therefore, it is critical to reveal the molecular mechanisms underlying grapevine (Vitis vinifera L.) drought stress tolerance useful to select new species with higher tolerance/resilience potentials. Drought-tolerant Tunisian local grapevine cultivar Razegui was exposed to water deficit for 16days. Subsequent ...
DOI:
10.1071/FP21026

21. Physiological defense and metabolic strategy of Pistia stratiotes in response to zinc-cadmium co-pollution

Author:
Yan Li; Jianpan Xin; Runan Tian
Source:
Plant physiology and biochemistry 2022 v.178 pp. 1-11
ISSN:
0981-9428
Subject:
Pistia stratiotes; absorption; amino acid metabolism; antioxidant activity; ascorbate peroxidase; ascorbic acid; bioaccumulation; cadmium; clones; energy; glutathione; glutathione dehydrogenase (ascorbate); glutathione-disulfide reductase; heat transfer; hydroponics; leaves; lipid metabolism; plant physiology; prostaglandins; sphingosine; zinc
Abstract:
... Pistia stratiotes is a cadmium (Cd) hyperaccumulating plant with strong bioaccumulation and translocation capacity for Cd. A hydroponic experiment was used to evaluate the combined effect of Zinc (Zn) and Cd at different concentrations on leaf growth and metabolism of P. stratiotes. This study revealed the physiological defense and metabolic strategy of responses to Zn–Cd co-pollution. With the Zn ...
DOI:
10.1016/j.plaphy.2022.02.020

22. Physiological, proteomic, and metabolomic analysis provide insights into Bacillus sp.-mediated salt tolerance in wheat

Author:
Yaguang Zhao; Fenghua Zhang; Bede Mickan; Dan Wang; Weichao Wang
Source:
Plant cell reports 2022 v.41 no.1 pp. 95-118
ISSN:
0721-7714
Subject:
Bacillus (bacteria); agricultural productivity; amino acid metabolism; biosynthesis; caffeine; catalase; chlorophyll; energy; galactose; gene expression regulation; glutathione; malondialdehyde; metabolites; metabolomics; palmitoleic acid; peroxidase; phenylalanine; phytomass; plant growth-promoting rhizobacteria; proline; proteomics; root inoculation; roots; rutin; salt stress; salt tolerance; sodium; soil salinization; superoxide dismutase; sustainable agriculture; toxicity; ubiquinones; wheat
Abstract:
... KEY MESSAGE: Herein, the inoculation with strain wp-6 promoted the growth of wheat seedlings by improving the energy production and conversion of wheat seedlings and alleviating salt stress. Soil salinization decreases crop productivity due to high toxicity of sodium ions to plants. Plant growth-promoting rhizobacteria (PGPR) have been demonstrated to alleviate salinity stress. However, the mechan ...
DOI:
10.1007/s00299-021-02788-0

23. Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders

Author:
Mohammad Afrouziyeh; Nicole M. Zukiwsky; Douglas R. Korver; Martin J. Zuidhof
Source:
Poultry science 2022 v.101 no.1 pp. 101532
ISSN:
0032-5791
Subject:
amino acid metabolism; biomarkers; biosynthesis; blood plasma; carnitine; choline; egg yolk; eggs; energy; feed intake; growth models; hens; lipids; liquid chromatography; liver; metabolites; metabolome; metabolomics; puberty; pullets; tandem mass spectrometry
Abstract:
... Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pullets were used, in which 30 pullets were randomly assigned to one of 10 unique growth trajectories ...
DOI:
10.1016/j.psj.2021.101532

24. Proteomics reveals the changes in energy metabolism associated with reproduction in the clam Meretrix petechialis

Author:
Di Wang; Baozhong Liu
Source:
Comparative biochemistry and physiology 2022 v.41 pp. 100954
ISSN:
1744-117X
Subject:
Meretrix; amino acid metabolism; carbohydrate metabolism; clams; energy; energy metabolism; females; glucose; hepatopancreas; males; muscles; proteome; proteomics; reproduction
Abstract:
... Because reproduction requires a considerable energy investment that presents metabolic challenges to animals, there is growing scientific interest in the effects of reproduction on metabolism. Here, the proteome of the hepatopancreas from female and male clams (Meretrix petechialis) before and after spawning was obtained using iTRAQ-based proteome analysis. Forty-two DEPs and 37 DEPs were detected ...
DOI:
10.1016/j.cbd.2021.100954

25. Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs

Author:
Gwendolyn J. Gregory; R. Kyle Bennett; Eleftherios T. Papoutsakis
Source:
Metabolic engineering 2022 v.71 pp. 99-116
ISSN:
1096-7176
Subject:
Escherichia coli; amino acid metabolism; biotransformation; carbon; energy; feedstocks; fermentation; gas production (biological); industrial applications; liquids; methane; methanol; methanotrophs; natural gas; synthetic biology
Abstract:
... Abundant natural gas reserves, along with increased biogas production, have prompted recent interest in harnessing methane as an industrial feedstock for the production of liquid fuels and chemicals. Methane can either be used directly for fermentation or first oxidized to methanol via biological or chemical means. Methanol is advantageous due to its liquid state under normal conditions. Methylotr ...
DOI:
10.1016/j.ymben.2021.09.005

26. Response of amino acid metabolism to decreased temperatures in anammox consortia: Strong, efficient and flexible

Author:
Jianhang Zhou; Tangran Huo; Jingqi Sun; Yiming Feng; Juejun Pan; Yunpeng Zhao; Sitong Liu
Source:
Bioresource technology 2022 v.352 pp. 127099
ISSN:
0960-8524
Subject:
amino acid metabolism; amino acids; anaerobic ammonium oxidation; biofilm; biosynthesis; energy; enzymes; lipids; metabolites; nitrogen; technology
Abstract:
... Although amino acid (AA) metabolism is basis of bacterial activities, unique characteristics of its response to decreased temperatures are not fully understood. Achieving nitrogen removal rate of 130–150 mg N/ (L∙d), metabolic differences of anammox consortia between 35 °C and four decreased temperatures (15–30 °C) were revealed respectively. 0-11.4-fold abundance variation of marker metabolites e ...
DOI:
10.1016/j.biortech.2022.127099

27. Short‐term metabolic responses of gilthead seabream, Sparus aurata, fed diets with different protein and protein: Energy levels

Author:
Renan Rosa Paulino; Alexandre Firmino Diógenes; Kátia Rodrigues Batista de Oliveira; Tamira Maria Orlando; Priscila Vieira Rosa; Helena Peres; Aires Oliva‐Teles
Source:
Aquaculture research 2022 v.53 no.4 pp. 1205-1217
ISSN:
1355-557X
Subject:
Sparus aurata; amino acid metabolism; amino acids; amylases; aquaculture; blood glucose; diet; energy; enzyme activity; fish; gluconeogenesis; glycogen; glycolysis; growth performance; lipid content; lipogenesis; research; starch
Abstract:
... The present study aimed to evaluate, in gilthead sea bream, the effect of dietary protein:energy (P/E) ratio and non‐protein energy (NPE) source on the post‐prandial profile of plasmatic parameters, hepatic energy reserves, enzymatic activities of digestive enzymes and hepatic key enzymes activities of the glycolytic (HK and GK), lipogenic (G6PDH and FAS), gluconeogenic (FBPase) and amino acid (GD ...
DOI:
10.1111/are.15654

28. Transcriptome profiling reveals the novel immunometabolism-related genes against WSSV infection from Fenneropenaeus merguiensis

Author:
Phattarunda Jaree; Pakpoom Boonchuen; Jumroensri Thawonsuwan; Hidehiro Kondo; Ikuo Hirono; Kunlaya Somboonwiwat
Source:
Fish & shellfish immunology 2022 v.120 pp. 31-44
ISSN:
1050-4648
Subject:
Fenneropenaeus merguiensis; RNA interference; White spot syndrome virus; amino acid metabolism; biochemical pathways; biosynthesis; death; energy; fish; fructose 6-phosphate; gene expression; glucose; glutamine; hemocytes; hexosamines; immune response; innate immunity; lipid metabolism; sequence analysis; shellfish; shrimp; shrimp culture; transcriptome; transcriptomics; virus replication; viruses
Abstract:
... The white spot syndrome virus (WSSV) has been considered a serious threat to shrimp aquaculture. Besides, the activation of cell metabolism as an immune reaction to the virus is now recognized as a piece of the pivotal puzzle of the antiviral responses. Hence, this study explores the relationship between metabolic gene expression and antiviral responses in shrimp using transcriptome analysis. The ...
DOI:
10.1016/j.fsi.2021.11.006

29. Transcriptomic analysis reveals the mechanism of host growth promotion by endophytic fungus of Rumex gmelinii Turcz

Author:
Changhong Ding; Shouyu Wang; Jiabin Li; Zhenyue Wang
Source:
Archives of microbiology 2022 v.204 no.7 pp. 443
ISSN:
0302-8933
Subject:
Plectosphaerella; Rumex; amino acid metabolism; auxins; biomass; carbohydrates; coculture; cytokinins; endophytes; energy; fungi; growth promotion; medicinal plants; plant growth; secondary metabolites; seedlings; stress tolerance; tissue culture; transcriptomics
Abstract:
... Rumex gmelinii Turcz. (RGT) is a medicinal plant of the genus Rumex, family Polygonaceae. Our research group isolated an endophytic fungus, Plectosphaerella cucumerina (Strain J-G) from RGT, which could significantly promote host growth when co-cultured with host seedlings. In this study, we used transcriptome analysis and verification experiments to explore the molecular mechanisms underlying thi ...
DOI:
10.1007/s00203-022-03072-9

30. Transcriptomic and proteomic analyses of far-red light effects in inducing shoot elongation in the presence or absence of paclobutrazol in Chinese pine

Author:
Yingtian Guo; Shihui Niu; Yousry A. El-Kassaby; Wei Li
Source:
Journal of forestry research 2022 v.33 no.3 pp. 1033-1043
ISSN:
1007-662X
Subject:
Pinus tabuliformis; amino acid metabolism; auxins; brassinolide; cell walls; conifers; energy; energy metabolism; ethylene; far-red light; forestry; gene expression regulation; gibberellins; paclobutrazol; proteomics; research; signal transduction; transcriptomics; white light
Abstract:
... In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced by paclobutrazol (PAC), yet the effects of far-red light in inducing shoot elongation in the presence or absence of PAC is poorly understood. In this study, transcriptomic and proteomi ...
DOI:
10.1007/s11676-021-01406-9

31. Understanding the role of biochar in affecting BDE-47 biodegradation by Pseudomonas plecoglossicida: An integrated analysis using chemical, biological, and metabolomic approaches

Author:
Xin Qi; Hua Yin; Minghan Zhu; Pengling Shao; Zhi Dang
Source:
Water research 2022 v.220 pp. 118679
ISSN:
0043-1354
Subject:
Pseudomonas plecoglossicida; aerobiosis; amino acid metabolism; biochar; biodegradation; cell proliferation; cytotoxicity; dicarboxylic acids; energy; metabolites; metabolomics; polymers; research; secretion; tricarboxylic acid cycle; water
Abstract:
... Biochar-assisted microbial degradation technology is considered as an important strategy to eliminate organic pollutants, but the mechanism of biochar in affecting biodegradation has not been systematically studied. To address this knowledge gap, the effect of various biochars on biodegradation of different initial concentrations of BDE-47 by Pseudomonas plecoglossicida was investigated. The resul ...
DOI:
10.1016/j.watres.2022.118679

32. Untargeted metabolomics study on the effects of rearing ducks in cages on bone quality

Author:
Qinglan Yang; Hehe Liu; Luyao Wang; Bin Wei; Qifan Wu; Qian Xu; Qian Tang; Jingjing Qi; Junpeng Li; Jianmei Wang; Jiwei Hu; Liang Li
Source:
Poultry science 2022 v.101 no.2 pp. 101604
ISSN:
0032-5791
Subject:
amino acid metabolism; ash content; blood serum; body weight; bone density; cages; calcium; citric acid; ducks; energy; energy metabolism; erucic acid; femur; humerus; lipid metabolism; metabolites; metabolome; metabolomics; models; osteoporosis; phosphorus; poultry industry; tibia
Abstract:
... The cage rearing model of the modern poultry industry makes the bones of birds more vulnerable to deterioration. In this study, at 8 wk of age, a total of 60 birds were randomly allocated to 2 groups, including the floor rearing group (FRD) and cage rearing group (CRD), and their body weight was measured every 2 wk. At the age of 20 wk, the tibia, femur, and humerus were collected from each group ...
DOI:
10.1016/j.psj.2021.101604

33. The combination of thymol and cinnamaldehyde reduces the survival and virulence of Listeria monocytogenes on autoclaved chicken breast

Author:
Siwei Liang; Xinyi Hu; Ruifei Wang; Meimei Fang; Yigang Yu; Xinglong Xiao
Source:
Journal of applied microbiology 2022 v.132 no.5 pp. 3937-3950
ISSN:
1364-5072
Subject:
Listeria monocytogenes; amino acid metabolism; autoclaving; betaine; biofilm; biosynthesis; breasts; carnitine; catabolism; chemotaxis; chickens; cysteine; energy; flagellum; gene expression regulation; glycerol; glycolysis; iron; microbiology; nucleic acids; pentoses; phosphates; pyruvic acid; ready-to-eat foods; thymol; transcriptomics; tricarboxylic acid cycle; virulence
Abstract:
... AIMS: To reveal the antibacterial mechanism of the combination of thymol and cinnamaldehyde to Listeria monocytogenes ATCC 19115 on autoclaved chicken breast. METHODS AND RESULTS: In this study, L. monocytogenes ATCC 19115 on autoclaved chicken breast was exposed to the stress of 125 μg/ml thymol and 125 μg/ml cinnamaldehyde, and transcriptome analysis was used to reveal the crucial antibacterial ...
DOI:
10.1111/jam.15496

34. The inhibitory effects of Ulva prolifera extracts on early growth of Spartina alterniflora and the underlying mechanisms

Author:
Yan Zhao; Yue Li; Yan Jin; Tao Cui; Sen Wang; Fanlong Kong
Source:
Journal of environmental management 2022 v.319 pp. 115639
ISSN:
0301-4797
Subject:
Spartina alterniflora; Ulva prolifera; allelochemicals; allelopathy; amino acid metabolism; biodiversity; economic development; ecosystems; energy; enzyme activity; ethyl acetate; hydrogels; invasive species; lipid peroxidation; methanol; photosynthesis; salinity; seed germination; seedling growth; seedlings; sequence analysis; survival rate; valine; waste utilization
Abstract:
... Spartina alterniflora, a highly invasive plant, has caused a serious threat to ecosystem biodiversity and economic development in coastal areas of many countries. In this study, the allelopathic effect of Ulva prolifera extracts on seed germination and seedling growth of S. alterniflora was studied. The results showed that three different treatments (water, methanol and ethyl acetate extract) coul ...
DOI:
10.1016/j.jenvman.2022.115639

35. Ammonium Ions Induce Cellulase Synthesis in Trichoderma koningii

Author:
Lirong Xiang; Yuanshan Lin; Yun Tian; Qiyou Liu; Liujun Chen; Zhijie Tan
Source:
Current microbiology 2021 v.78 no.8 pp. 3201-3211
ISSN:
0343-8651
Subject:
Trichoderma koningii; amino acid metabolism; ammonium; endo-1,4-beta-glucanase; energy; gene expression; gene expression regulation; gluconeogenesis; glycolysis; glycosides; mycelium; nitrogen; pyruvic acid; ribosomes
Abstract:
... Cellulase plays an important role in addressing the issue of the energy crisis. However, the yield and degradation efficiency of cellulase remain a major challenge. In the present study, we aimed to verify whether ammonium ion (NH₄⁺) could induce cellulase synthesis from T. koningii AS3.2774 and to explore new functional genes related to the cellulase production. Our results indicated that NH₄⁺ in ...
DOI:
10.1007/s00284-021-02568-9

36. Antimicrobial effect and mechanism of non-antibiotic alkyl gallates against Pseudomonas fluorescens on the surface of Russian sturgeon (Acipenser gueldenstaedti)

Author:
Run-run Zhang; Yu-gang Shi; Qing Gu; Mei Fang; Yue-wen Chen; Sheng Fang; Ya-Li Dang; Jian-she Chen
Source:
International journal of food microbiology 2021 v.342 pp. 109093
ISSN:
0168-1605
Subject:
Acipenser gueldenstaedtii; Pseudomonas fluorescens; amino acid metabolism; antibacterial properties; antibiotics; chitosan; energy; food microbiology; lipid bilayers; mechanism of action; oxygen consumption; refrigeration; shelf life; spoilage; sturgeon; tricarboxylic acid cycle
Abstract:
... Since Pseudomonas fluorescens is the main microorganism causing severe spoilage in refrigerated aquatic products, the searching for non-antibiotic antibacterial agents effective against it continues to receive increasing interest. This study aimed to investigate the antibacterial effects and mechanisms of alkyl gallic esters against P. fluorescens isolated from the Russian sturgeon (Acipenser guel ...
DOI:
10.1016/j.ijfoodmicro.2021.109093

37. Autonomous climbing: An effective exercise mode with beneficial outcomes of aerobic exercise and resistance training

Author:
Fei Shen; Yu Zhao; Wubin Ding; Kailin Liu; Xiangyu Ren; Qiang Zhang; Jian Yu; Yepeng Hu; Hui Zuo; Mingwei Guo; Ling Jin; Mingkai Gong; Wenhao Wu; Xuejiang Gu; Lingyan Xu; Fenglei Yang; Jian Lu
Source:
Life sciences 2021 v.265 pp. 118786
ISSN:
0024-3205
Subject:
Western blotting; amino acid metabolism; arginine; blood serum; body weight; energy; fatty acids; galactose; glucose; histochemistry; homeostasis; hypertrophy; landscapes; males; metabolomics; mitochondria; muscle development; muscles; oxidation; pentose phosphate cycle; proline; quantitative polymerase chain reaction; tyrosine; weight control
Abstract:
... To assess the effects of three specific exercise training modes, aerobic exercise (A), resistance training (R) and autonomous climbing (AC), aimed at proposing a cross-training method, on improving the physical, molecular and metabolic characteristics of mice without many side effects.Seven-week-old male mice were randomly divided into four groups: control (C), aerobic exercise (A), resistance tra ...
DOI:
10.1016/j.lfs.2020.118786

38. Bacterial diversity in intestinal mucosa of mice fed with Dendrobium officinale and high-fat diet

Author:
Xiaoya Li; Xinxin Peng; Kangxiao Guo; Zhoujin Tan
Source:
3 Biotech 2021 v.11 no.1 pp. 22
ISSN:
2190-572X
Subject:
Bacteroidetes; Bifidobacterium; Dendrobium officinale; Ochrobactrum; Ruminococcus; Verrucomicrobia; amino acid metabolism; carbohydrates; energy; flora; high fat diet; intestinal microorganisms; intestinal mucosa; models; placebos
Abstract:
... This study aimed to explore the effect of Dendrobium officinale (DO) on the diversity of intestinal mucosal flora in high-fat diet mice and provided an experimental basis for the development and research of DO and its series products. Twenty-four mice were randomly assigned to four equal groups of six mice, namely the control (bcm) group, model (bmm) group, Dendrobium officinale (bdm) group, and p ...
DOI:
10.1007/s13205-020-02558-x

39. Comparative genomic analysis of hyper-ammonia producing Acetoanaerobium sticklandii DSM 519 with purinolytic Gottschalkia acidurici 9a and pathogenic Peptoclostridium difficile 630

Author:
C. Sangavai; P. Chellapandi
Source:
Genomics 2021 v.113 no.6 pp. 4196-4205
ISSN:
0888-7543
Subject:
Clostridia; amino acid metabolism; amino acids; ancestry; biofuels; carbon; computer simulation; energy; evolution; genes; genomics; hydrogen production; proteolysis
Abstract:
... Acetoanaerobium sticklandii DSM519 (CST) is a hype-ammonia producing non-pathogenic anaerobe that can use amino acids as important carbon and energy sources through the Stickland reactions. Biochemical aspects of this organism have been extensively studied, but systematic studies addressing its metabolic discrepancy remain scant. In this perspective, we have intensively analyzed its genomic and me ...
DOI:
10.1016/j.ygeno.2021.11.010

40. Comparative study on the metabolome of the most prevalent fish pathogen, Streptococcus iniae at different temperatures using 1H nuclear magnetic resonance

Author:
Mozhde Mardanipuor; Ruhollah Rahimi; Saeed Hajirezaee; Farzaneh Nikookhah
Source:
Aquaculture research 2021 v.52 no.10 pp. 4745-4751
ISSN:
1355-557X
Subject:
Oncorhynchus mykiss; Streptococcus iniae; acetone; acetylcysteine; amino acid metabolism; analysis of variance; aquaculture; betaine; comparative study; energy; fermentation; fish; glutamine; homeostasis; metabolites; metabolome; nuclear magnetic resonance spectroscopy; pathogenicity; pathogens; pyruvic acid; research; temperature; tyrosine
Abstract:
... Streptococcus iniae is known as a most common pathogen in rainbow trout farms, which its pathogenicity depends strongly on temperature. This study was performed to identify the metabolic responses of the Streptococcus iniae at different temperatures (22, 18, 14, 10°C). For this purpose, the samples of the bacteria were cultured at experimental temperatures in three replicates. After metabolite ext ...
DOI:
10.1111/are.15308

41. Comprehensive analysis of metabolic changes in rats exposed to acrylamide

Author:
Shiyuan Zhao; Haitao Zhong; Chunmei Geng; Hongjia Xue; Changshui Wang; Wenxue Sun; Ruili Dang; Wenxiu Han; Pei Jiang
Source:
Environmental pollution 2021 v.287 pp. 117591
ISSN:
0269-7491
Subject:
acrylamides; amino acid metabolism; amino acids; biomarkers; blood serum; carcinogenicity; cortex; energy; energy metabolism; fatty acids; heart; hippocampus; humans; inflammation; kidneys; liver; males; metabolites; metabolomics; mutagens; neurotoxicity; oxidative stress; pollution; reproductive toxicology; soil; urine
Abstract:
... Acrylamide (ACR) is a widely used environmentally hazardous compound that is known to be neurotoxic, genotoxic, carcinogenic, and reproductive toxicity. It is widely present in soil, water, agents used in chemical industries, and food. It can be distributed to all organs and tissues, and can cause damage to various human systems and those of other animals. Previous metabolomics studies have mainly ...
DOI:
10.1016/j.envpol.2021.117591

42. Corticosterone-Mediated Physiological Stress Alters Liver, Kidney, and Breast Muscle Metabolomic Profiles in Chickens

Author:
Catherine L. J. Brown; Sarah J. M. Zaytsoff; Tony Montina; G. Douglas Inglis
Source:
Animals 2021 v.11 no.11 pp. -
ISSN:
2076-2615
Subject:
amino acid metabolism; amino acids; anabolism; breast muscle; carnosine; catabolism; corticosterone; energy; ethanol; galactose; gluconeogenesis; glucose; glycosylation; kidneys; liver; mannose; meat; metabolites; metabolome; metabolomics; nitrogen metabolism; stress response
Abstract:
... The impact of physiological stress on the metabolomes of liver, kidney, and breast muscle was investigated in chickens. To incite a stress response, birds were continuously administered corticosterone (CORT) in their drinking water at three doses (0, 10, and 30 mg L⁻¹), and they were sampled 1, 5, and 12 days after the start of the CORT administration. To solubilize CORT, it was first dissolved in ...
DOI:
10.3390/ani11113056

43. Distinct metabolic shifts occur during the transition between normoxia and hypoxia in the hybrid and its maternal abalone

Author:
Yawei Shen; Ying Zhang; Qizhen Xiao; Yang Gan; Yi Wang; Gewen Pang; Zekun Huang; Feng Yu; Xuan Luo; Caihuan Ke; Weiwei You
Source:
Science of the total environment 2021 v.794 pp. 148698
ISSN:
0048-9697
Subject:
Haliotis discus hannai; abalone; amino acid metabolism; aquaculture; biomarkers; carbohydrate metabolism; climate change; dissolved oxygen; energy; energy metabolism; glutamic acid; glycogen; hemolymph; hepatopancreas; hybrids; hypoxia; metabolites; metabolomics; normoxia; stress tolerance; summer
Abstract:
... Due to anthropogenic activities that have increased global climate change and nutrient discharges, severe hypoxic events have frequently occurred in coastal waters in recent years. Relying on coastal waters, the aquaculture area has suffered ecological and economic losses caused by hypoxia, especially in summer. In this study, to investigate the stress resistance of the Pacific abalone Haliotis di ...
DOI:
10.1016/j.scitotenv.2021.148698

44. Effect of dietary taurine supplementation on metabolome variation in plasma of Nile tilapia

Author:
G.P. Shen; Z.N. Ding; T. Dai; J.H. Feng; J.Y. Dong; F. Xia; J.J. Xu; J.D. Ye
Source:
Animal 2021 v.15 no.3 pp. 100167
ISSN:
1751-7311
Subject:
Oreochromis niloticus; absorption; amino acid metabolism; aquaculture; carbohydrates; digestion; energy; fish; growth and development; magnetism; metabolites; metabolome; metabolomics; nucleotides; taurine
Abstract:
... Taurine has been considered as an essential nutrient for many aquaculture species. While dietary taurine supplementation is highly recommended, novelty studies on taurine metabolism in fish are needed. The present study aimed to provide insight into the molecular mechanisms involved in multiple metabolome changes in Nile tilapia (Oreochromis niloticus) by studying plasma metabolic profile changes ...
DOI:
10.1016/j.animal.2020.100167

45. Effect of oxygen concentrations and branched‐chain amino acids on the growth and development of sub‐seafloor fungus, Schizophyllum commune 20R‐7‐F01

Author:
Muhammad Zain Ul Arifeen; Zhi‐Jun Ma; Si Wu; Jun‐Zhong Liu; Ya‐Rong Xue; Chang‐Hong Liu
Source:
Environmental microbiology 2021 v.23 no.11 pp. 6940-6952
ISSN:
1462-2912
Subject:
Schizophyllum commune; amino acid metabolism; energy; fruiting bodies; fruits; fungi; growth and development; hypoxia; mitochondria; oxygen; sediments
Abstract:
... Fungi have been reported to be the dominant eukaryotic group in anoxic sub‐seafloor sediments, but how fungi subsist in the anoxic sub‐marine sedimental environment is rarely understood. Our previous study demonstrated that the fungus, Schizophyllum commune 20R‐7‐F01 isolated from a ~2 km sediment below the seafloor, can grow and produce primordia in the complete absence of oxygen with enhanced pr ...
DOI:
10.1111/1462-2920.15738

46. Effects of starvation stress on jade perch scortum barcoo based on proteomics analysis

Author:
Xiaoyong Xie; Guoling Ye; Yuyuan Bao; Ziwei Ying; Mujiao Xie; Changbo Zhu; Ruixuan Wang
Source:
Aquaculture research 2021 v.52 no.6 pp. 2840-2851
ISSN:
1355-557X
Subject:
Scortum barcoo; amino acid metabolism; antioxidant activity; antioxidants; aquaculture; energy; fasting; fatty acid metabolism; fish; gene expression regulation; oxidative phosphorylation; proteomics; research; stable isotopes; starvation; tandem mass spectrometry
Abstract:
... Proteins related to antioxidant defence systems and energy maintenance play a vital role in resisting starvation in fish. However, studies on the underlying molecular mechanism of starvation stress in the jade perch Scortum barcoo are very limited. Thus, the proteomic changes in S. barcoo during fasting were investigated by stable isotope labelling and high‐performance liquid chromatography‐tandem ...
DOI:
10.1111/are.15135

47. Floral organ-specific proteome profiling of the floral ornamental orchid (Cymbidium goeringii) reveals candidate proteins related to floral organ development

Author:
Yue Chen; Zihan Xu; Qi Shen; Chongbo Sun
Source:
Botanical studies 2021 v.62 no.1 pp. 23
ISSN:
1999-3110
Subject:
Cymbidium goeringii; amino acid metabolism; aminocyclopropanecarboxylate oxidase; energy; flowering; gene expression regulation; gibberellins; hydrolases; lipid metabolism; morphogenesis; ornamental plants; peptides; photosynthesis; proteome; proteomics
Abstract:
... BACKGROUND: Cymbidium goeringii, belonging to the Orchidaceae family, is an important ornamental plant with striking petals and lips. Extremely diversified floral patterns and morphologies make C. goeringii good research material to examine floral development of orchids. However, no floral organ-specific protein has been identified yet. To screen floral development associated proteins, four proteo ...
DOI:
10.1186/s40529-021-00330-9

48. GC–MS‐based metabolomics reveals metabolic changes in overwintering scylla paramamosain at two different salinities

Author:
Junming Zhou; Na Li; Yuanyuan Fu; Congying He; Kuanhong Meng; Yanrong Li; Zhujun Zhu; Lili Huang; Daifu Hu; Chunlin Wang; Lei Liu
Source:
Aquaculture research 2021 v.52 no.7 pp. 3110-3123
ISSN:
1355-557X
Subject:
Scylla paramamosain; amino acid metabolism; aquaculture; carbohydrate metabolism; docosahexaenoic acid; energy; fumaric acid; glucose 6-phosphate; hepatopancreas; lactic acid; lipid metabolism; lysine; metabolites; metabolomics; overwintering; palmitic acid; research; salinity; urea; weight gain
Abstract:
... Overwintering is an important part of the life cycle of Scylla paramamosain. Salinity has a significant effect on the growth and metabolism of overwintering S. paramamosain. 4‰ and 25‰ are typical salinities in different regions where S. paramamosain overwinters. In order to find the optimum salinity for overwintering, the metabolites in overwintering S. paramamosain at salinities of 4‰ and 25‰ we ...
DOI:
10.1111/are.15157

49. Genomic and transcriptomic analysis of Bacillus subtilis JNFE1126 with higher nattokinase production through ultraviolet combined 60Co-γ ray mutagenesis

Author:
Yuxue Wang; Jing Wang; Xuan Zhang; Yanjun Tong; Ruijin Yang
Source:
Lebensmittel-Wissenschaft + Technologie 2021 v.147 pp. 111652
ISSN:
0023-6438
Subject:
Bacillus subtilis; amino acid metabolism; energy; gene expression regulation; genomics; mutagenesis; mutants; serine proteinases; transcription (genetics); transcriptomics
Abstract:
... Nattokinase is a serine protease which is produced by Bacillus subtilis and exhibits vigorous fibrinolytic activity. Nattokinase is useful for preventing and treating thromboembolic diseases. In this study, the effect of ultraviolet combined ⁶⁰Co-γ ray on B. subtilis were investigated, and the mechanism of NK production enhancement was elucidated at molecular level. There was obvious differences o ...
DOI:
10.1016/j.lwt.2021.111652

50. Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism

Author:
Min Feng; Shigang Fei; Junming Xia; Mengmeng Zhang; Hongyun Wu; Luc Swevers; Jingchen Sun
Source:
Viruses 2021 v.13 no.5 pp. -
ISSN:
1999-4915
Subject:
Bombyx mori; Bombyx mori nucleopolyhedrovirus; amino acid metabolism; amino acids; biosynthesis; carbon metabolism; energy; glycolysis; hemolymph; metabolomics; silkworms; virus replication
Abstract:
... Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Infection of the silkworm, Bombyx mori, by Bombyx mori nucleopolyhedrovirus (BmNPV), has been studied extensively in the past to unravel interactions between baculoviruses and their lepidopteran hosts. To understand the interaction between the host metabolic responses ...
DOI:
10.3390/v13050841

51. Heat stress alters muscle protein and amino acid metabolism and accelerates liver gluconeogenesis for energy supply in broilers

Author:
Bingbing Ma; Lin Zhang; Jiaolong Li; Tong Xing; Yun Jiang; Feng Gao
Source:
Poultry science 2021 v.100 no.1 pp. 215-223
ISSN:
0032-5791
Subject:
amino acid metabolism; average daily gain; blood serum; breast muscle; corticosterone; energy; enzymes; feed intake; gene expression; genes; gluconeogenesis; glycine (amino acid); growth performance; heat stress; liver; lysine; messenger RNA; muscle protein; muscle tissues; muscular atrophy; ribosomal proteins; solutes; threonine; tyrosine
Abstract:
... Heat stress impairs growth performance and alters body protein and amino acid metabolism. This study was investigated to explore how body protein and amino acid metabolism changed under heat stress (HS) and the stress adaptation mechanism. A total of 144 broilers (28 d old) were divided into 3 treatment groups for 1 wk: HS group (32°C), normal control group (22°C), and pair-feeding group (22°C). W ...
DOI:
10.1016/j.psj.2020.09.090

52. Hepatic transcriptomic adaptation from prepartum to postpartum in dairy cows

Author:
S.T. Gao; D.D. Girma; M. Bionaz; L. Ma; D.P. Bu
Source:
Journal of dairy science 2021 v.104 no.1 pp. 1053-1072
ISSN:
0022-0302
Subject:
Holstein; RNA; adipokines; amino acid metabolism; amino acids; beta oxidation; bioinformatics; cell proliferation; cholesterol metabolism; dairy science; energy; gluconeogenesis; glucose; lactation; lipids; lipogenesis; liver; microarray technology; mitochondria; peroxisome proliferator-activated receptors; pregnancy; protein synthesis; transcriptomics; xenobiotics
Abstract:
... The transition from pregnancy to lactation is the most challenging period for high-producing dairy cows. The liver plays a key role in biological adaptation during the peripartum. Prior works have demonstrated that hepatic glucose synthesis, cholesterol metabolism, lipogenesis, and inflammatory response are increased or activated during the peripartum in dairy cows; however, those works were limite ...
DOI:
10.3168/jds.2020-19101

53. Impact of CO2 overpressure on yeast mitochondrial associated proteome during the “prise de mousse” of sparkling wine production

Author:
Juan Antonio Porras–Agüera; Jaime Moreno–García; Teresa García–Martínez; Juan Moreno; Juan Carlos Mauricio
Source:
International journal of food microbiology 2021 v.348 pp. 109226
ISSN:
0168-1605
Subject:
Saccharomyces cerevisiae; amino acid metabolism; carbon dioxide; electron transport chain; energy; ethanol; fermentation; food microbiology; metabolomics; mitochondria; multivariate analysis; proteome; proteomics; starvation; transcriptomics; tricarboxylic acid cycle; viability; wine industry; winemaking; yeasts
Abstract:
... The “prise de mousse” stage during sparkling wine elaboration by the traditional method (Champenoise) involves a second fermentation in a sealed bottle followed by a prolonged aging period, known to contribute significantly to the unique organoleptic properties of these wines. During this stage, CO₂ overpressure, nutrient starvation and high ethanol concentrations are stress factors that affect ye ...
DOI:
10.1016/j.ijfoodmicro.2021.109226

54. In Vitro Characterization and Metabolomic Analysis of Cold-Stored Platelets

Author:
HanQi Wayne Zhao; Katherine Serrano; Davide Stefanoni; Angelo D’Alessandro; Dana V. Devine
Source:
Journal of proteome research 2021 v.20 no.5 pp. 2251-2265
ISSN:
1535-3907
Subject:
agitation; ambient temperature; amino acid metabolism; bacterial growth; beta oxidation; cold; cold storage; energy; glutathione; lactadherin; metabolites; metabolomics; oxidative stress; pentoses; phosphates; proteome; research; risk; storage temperature
Abstract:
... Platelet concentrates are currently stored at room temperature (RP) under constant agitation for up to 5–7 days depending on national regulations. However, platelet quality deteriorates during storage and room-temperature storage also increases the risk of bacterial growth. Previous studies have shown that cold-stored platelets (CPs) have higher hemostatic functions and can be stored for up to 3 w ...
DOI:
10.1021/acs.jproteome.0c00792

55. Inferring catabolism through analysis of amino acid balance in Vicia faba L. seedlings

Author:
Thiago Batista Moreira; Thomas Christopher Rhys Williams
Source:
Revista brasileira de botânica 2021 v.44 no.4 pp. 859-868
ISSN:
0100-8404
Subject:
Vicia faba; amino acid metabolism; biosynthesis; carbon; catabolism; energy; histidine; metabolites; phenylalanine; tricarboxylic acid cycle
Abstract:
... Whilst carbohydrates are the principal respiratory substrate in plants, under certain circumstances amino acids may be catabolised in order to produce energy, reducing equivalents and precursors for biosynthesis. This situation may occur during the phase of reserve mobilisation in seedlings, and this period of development therefore represents an opportunity to identify which amino acids are in fac ...
DOI:
10.1007/s40415-021-00755-3

56. Integrated transcriptome and metabolome analysis reveals molecular responses of the clams to acute hypoxia

Author:
Xiujun Sun; Kang Tu; Li Li; Biao Wu; Lei Wu; Zhihong Liu; Liqing Zhou; Jiteng Tian; Aiguo Yang
Source:
Marine environmental research 2021 v.168 pp. 105317
ISSN:
0141-1136
Subject:
Ruditapes philippinarum; amino acid metabolism; aquaculture; biomarkers; biomineralization; cell proliferation; energy; habitats; hypoxia; littoral zone; metabolites; metabolomics; neurons; osmoregulation; oxygen; research; risk; shellfish; transcriptome; transcriptomics
Abstract:
... Mudflat shellfish have evolved well-adapted strategies for coping with dynamic environmental fluxes and stressful conditions, including oxygen availability. The Manila clams Ruditapes philippinarum are worldwide cultured shellfish in marine intertidal zone, which usually encounter great risk of acute hypoxia exposure in coastal habitats. To reveal the effects of acute hypoxia on metabolic changes ...
DOI:
10.1016/j.marenvres.2021.105317

57. Investigation of the idiosyncratic hepatotoxicity of Polygonum multiflorum Thunb. through metabolomics using GC-MS

Author:
Yan Lin; Rong Xiao; Bo-hou Xia; Zhi-min Zhang; Chun Li; Ping Wu; Duan-fang Liao; Li-mei Lin
Source:
BMC complementary medicine and therapies 2021 v.21 no.1 pp. 120
ISSN:
2662-7671
Subject:
3-hydroxybutyric acid; Reynoutria multiflora; alternative medicine; amino acid metabolism; biomarkers; blood serum; discriminant analysis; energy; energy metabolism; eosin; ethanol; galactose; glucose; glucosides; hepatotoxicity; hexanoic acid; lipid metabolism; liver; liver function; malic acid; mannose; metabolites; metabolomics; oxalates; oxalic acid; palmitic acid; phosphoric acid; proline; rats; ribose; urea; valine
Abstract:
... BACKGROUND: The idiosyncratic hepatotoxicity of Polygonum multiflorum (PM) has attracted considerable interest, but the idiosyncratically hepatotoxic components and endogenous metabolite changes resulting from idiosyncratic hepatotoxicity of PM are not well understood. The aim of this study was to identify the idiosyncratically hepatotoxic components and potential endogenous metabolic biomarkers f ...
DOI:
10.1186/s12906-021-03276-4

58. Involvement of energy metabolism and amino acid metabolism in quality attributes of postharvest Pleurotus eryngii treated with a novel phase change material

Author:
Dong Li; Di Wang; Yida Fang; Tarun Belwal; Li Li; Xingyu Lin; Yanqun Xu; Hangjun Chen; Ming Zhu; Zisheng Luo
Source:
Postharvest biology and technology 2021 v.173 pp. 111427
ISSN:
0925-5214
Subject:
Pleurotus eryngii; adenosinetriphosphatase; ambient temperature; amino acid metabolism; antioxidant activity; catabolism; cold chain; cytochrome-c oxidase; energy; energy metabolism; flavonoids; glutamic acid; ice; latent heat; phase transition; phenylalanine; postharvest storage; proline; succinate dehydrogenase (quinone); transportation
Abstract:
... Phase change material (PCM) is a substance which absorbs latent heat by a phase transition, and it plays important roles in short-time cold chain transportation. In the present study, we developed a novel water-based PCM, which showed a better effect on maintaining low-temperature conditions compared with ice, and applied in postharvest storage of Pleurotus eryngii at room temperature for 5 d. The ...
DOI:
10.1016/j.postharvbio.2020.111427

59. Metabolomic analysis of honey bees (Apis mellifera) response to carbendazim based on UPLC-MS

Author:
Heng Chen; Kang Wang; Wenna Ji; Hao Xu; Yibing Liu; Shuang Wang; Zhi Wang; Fuchao Gao; Zheguang Lin; Ting Ji
Source:
Pesticide biochemistry and physiology 2021 v.179 pp. 104975
ISSN:
0048-3575
Subject:
Apis mellifera; aflatoxins; amino acid metabolism; apiculture; brain; carbendazim; energy; fungicides; glycerol; growth and development; honey; honey bees; immune system; industry; metabolites; metabolome; metabolomics; models
Abstract:
... Pesticides are one of the main causes of colony losses globally, posing a huge threat to the beekeeping industry. The fungicide carbendazim is commonly used on many crops worldwide, but the effects of fungicides on honey bees have received less attention than those of insecticides. Previous studies have shown that sublethal doses of carbendazim hinder growth and development and may destabilize and ...
DOI:
10.1016/j.pestbp.2021.104975

60. Metabolomic changes in the liver tissues of cows in early lactation supplemented with dietary rumen-protected glucose during the transition period

Author:
X.P. Li; Z.L. Tan; Z.C. Li; S. Gao; K.L. Yi; C.S. Zhou; S.X. Tang; X.F. Han
Source:
Animal feed science and technology 2021 v.281 pp. 115093
ISSN:
0377-8401
Subject:
Holstein; amino acid metabolism; body weight; diet; early lactation; energy; feeds; gene expression regulation; gluconeogenesis; glucose; glycolysis; liquid chromatography; liver; metabolites; metabolomics; milk yield; pentose phosphate cycle; riboflavin; serine; tandem mass spectrometry; technology; threonine; tryptophan
Abstract:
... A fat-coated rumen-protected glucose (RPG) product was used to meet the high energy requirements of transition-period cows. Twelve multiparous Holstein cows (age, 4–5 years; body weight, 515 ± 42 kg; milk yield, 16.1 ± 3.7 kg/d) were blocked by body weight, previous milk production, and calving date and were assigned to two groups: the control (CON) group and the RPG group. For treatment, the CON ...
DOI:
10.1016/j.anifeedsci.2021.115093

61. Metabolomic profiling reveals the effects of early-life lactoferrin intervention on protein synthesis, energy production and antioxidative capacity in the liver of suckling piglets

Author:
Ping Hu; Fangzhou Zhao; Jing Wang; Weiyun Zhu
Source:
Food & function 2021 v.12 no.8 pp. 3405-3419
ISSN:
2042-650X
Subject:
amino acid metabolism; amino acids; antioxidant activity; body weight; energy; gene expression; genes; jejunum; lactoferrin; liver; malondialdehyde; metabolomics; mitochondria; neonates; non-specific serine/threonine protein kinase; phosphorylation; protein synthesis; proteolysis; serum albumin; tricarboxylic acid cycle; urea nitrogen
Abstract:
... This study aimed to determine the effects of an early-life lactoferrin (LF) intervention on liver metabolism in suckling piglets. Sixty newborn piglets with an average initial body weight (BW) of 1.51 ± 0.05 kg were assigned to a control (CON) group and an LF group. At age 1 to 7 days, the piglets in the LF group were orally administered LF solution (0.5 g per kg BW daily), whereas the piglets in ...
DOI:
10.1039/d0fo01747g

62. Metabonomics of white adipose tissue and brown adipose tissue in Tupaia belangeri during cold acclimation

Author:
Dong-min Hou; Ting Jia; Qi Li; Zheng-kun Wang; Wan-long Zhu
Source:
Comparative biochemistry and physiology 2021 v.38 pp. 100823
ISSN:
1744-117X
Subject:
Tupaia belangeri; acclimation; amino acid metabolism; brown adipose tissue; chemical composition; cold; cold stress; dicarboxylic acids; energy; homeostasis; lipid metabolism; liquid chromatography; metabolites; metabolomics; pyruvic acid; spectrometers; sugars; temperature; tricarboxylic acid cycle; white adipose tissue
Abstract:
... In the present study, liquid chromatography-mass spectrometer (LC-MS) was used to perform untargeted metabolomics analysis of white adipose tissue (WAT) and brown adipose tissue (BAT) in Tupaia belangeri during cold acclimation. Differences in biochemical composition between WAT and BAT were compared. Clarifying how the two adipose tissues respond to the lower temperature in terms of metabolomics, ...
DOI:
10.1016/j.cbd.2021.100823

63. Metallothionein Attenuated Arsenic-Induced Cytotoxicity: The Underlying Mechanism Reflected by Metabolomics and Lipidomics

Author:
Zihe Qi; Qinghong Wang; Haitao Wang; Mingqian Tan
Source:
Journal of agricultural and food chemistry 2021 v.69 no.18 pp. 5372-5380
ISSN:
1520-5118
Subject:
amino acid metabolism; arsenic; biochemical pathways; cell viability; chelation; cysteine; cytotoxicity; energy; food chemistry; lipidomics; membrane potential; metabolic diseases; metallothionein; mitochondrial membrane; phospholipids; reactive oxygen species
Abstract:
... Arsenic ions (As³⁺) have been recognized as a hazard that threatens the health of humans. Metallothionein (MT) rich in cysteine may provide favorable binding sites for chelation of As³⁺. However, the influence of MT on As³⁺-induced toxicity and the underlying mechanism are poorly understood, especially at the metabolic level. Herein, the effects of MT on As³⁺-induced toxicity were evaluated. Cell ...
DOI:
10.1021/acs.jafc.1c00724

64. Physiological and Proteomic Responses of Pitaya to PEG-Induced Drought Stress

Author:
Aihua Wang; Chao Ma; Hongye Ma; Zhilang Qiu; Xiaopeng Wen
Source:
Agriculture (Basel) 2021 v.11 no.7 pp. -
ISSN:
2077-0472
Subject:
Hylocereus polyrhizus; agriculture; amino acid metabolism; antioxidant enzymes; arginine; aspartic acid; cultivars; drought; drought tolerance; energy; glutamic acid; glutathione; lipid peroxidation; photosynthesis; polyethylene glycol; protein synthesis; proteome; proteomics; secondary metabolites; sugars; water stress
Abstract:
... Pitaya (Hylocereus polyrhizus L.) is highly tolerant to drought stress. Elucidating the response mechanism of pitaya to drought will substantially contribute to improving crop drought tolerance. In the present study, the physiological and proteomic responses of the pitaya cultivar ‘Zihonglong’ were compared between control seedlings and seedlings exposed to drought stress (−4.9 MPa) induced by pol ...
DOI:
10.3390/agriculture11070632

65. Physiological and transcriptome analysis of γ-aminobutyric acid (GABA) in improving Gracilariopsis lemaneiformis stress tolerance at high temperatures

Author:
Shixia Liu; Jun Zhang; Chaoyang Hu; Xue Sun; Nianjun Xu
Source:
Algal research 2021 v.60 pp. 102532
ISSN:
2211-9264
Subject:
Gracilariopsis lemaneiformis; algae; amino acid metabolism; calmodulin; catalase; energy; energy metabolism; gene expression; heat stress; heat tolerance; homeostasis; peroxidase; phycobiliprotein; polyamines; proline; protein kinases; research; stress tolerance; summer; superoxide dismutase; thallus; transcription (genetics); transcriptomics; tricarboxylic acid cycle; China
Abstract:
... Gracilariopsis lemaneiformis, an economically important red seaweed in China, is currently undergoing high-temperature (HT) stress due to global warming. To alleviate summertime HT stress, algae can acquire resistance through metabolism reprogramming and modulation of stress-related gene expression. γ-Aminobutyric acid (GABA), a non-protein four‑carbon amino acid, plays an important role in regula ...
DOI:
10.1016/j.algal.2021.102532

66. Proteome changes in pepper (Capsicum annuum L.) leaves induced by the green peach aphid (Myzus persicae Sulzer)

Author:
Victoria Florencio-Ortiz; Susana Sellés-Marchart; José L. Casas
Source:
BMC plant biology 2021 v.21 no.1 pp. 12
ISSN:
1471-2229
Subject:
Capsicum annuum; Myzus persicae; amino acid metabolism; bioinformatics; carbohydrates; energy; gene expression regulation; hormone metabolism; leaves; lipids; oxidative stress; pepper; photorespiration; proteome; proteomics; toxicity
Abstract:
... BACKGROUND: Aphid attack induces defense responses in plants activating several signaling cascades that led to the production of toxic, repellent or antinutritive compounds and the consequent reorganization of the plant primary metabolism. Pepper (Capsicum annuum L.) leaf proteomic response against Myzus persicae (Sulzer) has been investigated and analyzed by LC-MS/MS coupled with bioinformatics t ...
DOI:
10.1186/s12870-020-02749-x

67. Proteomic analysis of liver tissue between fat and lean broiler lines

Author:
C. Wu; C. Chen; Y. Liu; H. Li; B. Cheng
Source:
British poultry science 2021 v.62 no.2 pp. 211-218
ISSN:
1466-1799
Subject:
abdominal fat; adipose tissue; agricultural colleges; amino acid metabolism; cytoskeleton; energy; fatty acids; fluorescence; gel electrophoresis; gene expression regulation; lipid content; lipid metabolism; liver; models; oxidative stress; protein synthesis; proteomics
Abstract:
... 1. The liver is the major site of fatty acid synthesis in chickens. Lipid metabolism in the liver correlates with the deposition of triglycerides in adipose tissue. Northeast Agricultural University broilers lines divergently selected for abdominal fat content (NEAUHLF) provide a unique model to study the mechanisms of fat deposition. 2. In previous studies, differentially expressed genes (DEGs) i ...
DOI:
10.1080/00071668.2020.1847253

68. Rumen Fermentation—Microbiota—Host Gene Expression Interactions to Reveal the Adaptability of Tibetan Sheep in Different Periods

Author:
Weibing Lv; Xiu Liu; Yuzhu Sha; Hao Shi; Hong Wei; Yuzhu Luo; Jiqing Wang; Shaobin Li; Jiang Hu; Xinyu Guo; Xiaoning Pu
Source:
Animals 2021 v.11 no.12 pp. -
ISSN:
2076-2615
Subject:
Bacteroidetes; Firmicutes; acetates; altitude; amino acid metabolism; butyrates; endo-1,4-beta-glucanase; energy; epithelium; fermentation; gene expression; glucose; homeostasis; microbiome; nutrient uptake; oxygen; propionic acid; reproduction; rumen; sheep; sodium; temperature; tight junctions; xylanases; China
Abstract:
... As an important ruminant on the Qinghai-Tibet Plateau, Tibetan sheep can maintain their population reproduction rate in the harsh high-altitude environment of low temperature and low oxygen, which relies on their special plateau adaptations mechanism that they have formed for a long time. Microbiomes (known as “second genomes”) are closely related to the nutrient absorption, adaptability, and heal ...
DOI:
10.3390/ani11123529

69. Salinity-dependent effects of ZnO nanoparticles on bioenergetics and intermediate metabolite homeostasis in a euryhaline marine bivalve, Mytilus edulis

Author:
Mirza Nusrat Noor; Fangli Wu; Eugene P. Sokolov; Halina Falfushynska; Stefan Timm; Fouzia Haider; Inna M. Sokolova
Source:
Science of the total environment 2021 v.774 pp. 145195
ISSN:
0048-9697
Subject:
Mytilus edulis; amino acid metabolism; anaerobiosis; energy; environment; euryhaline species; free amino acids; habitats; homeostasis; metabolites; mitochondria; salinity; salt stress; toxicity; Baltic Sea
Abstract:
... Engineered nanoparticles including ZnO nanoparticles (nZnO) are important emerging pollutants in aquatic ecosystems creating potential risks to coastal ecosystems and associated biota. The toxicity of nanoparticles and its interaction with the important environmental stressors (such as salinity variation) are not well understood in coastal organisms and require further investigation. Here, we exam ...
DOI:
10.1016/j.scitotenv.2021.145195

70. Shifts in diversity and function of bacterial community during manufacture of rushan

Author:
Yang Jiang; Peng Yu; Xiaoming Liu; Jianxin Zhao; Hao Zhang; Wei Chen
Source:
Journal of dairy science 2021 v.104 no.12 pp. 12375-12393
ISSN:
0022-0302
Subject:
Acetobacter; Lactobacillus amylolyticus; Lactobacillus helveticus; Lactococcus; Streptococcus; amino acid metabolism; bacterial communities; cysteine; dairy science; energy; lysine; manufacturing; methionine; raw milk; volatile compounds; whey; xylene; China
Abstract:
... Rushan is a traditional dairy product consumed by the Bai people in the Yunnan Province of China, and its production still follows the traditional procedure of backslopping. However, how the microbial composition of raw materials and processing shape the microorganisms in rushan have not been systemically reported. In this study, high-throughput sequencing technique was applied to analyze the micr ...
DOI:
10.3168/jds.2021-20654

71. Transcriptome analysis reveals hepatopancreatic genes and pathways associated with metabolism responding to water salinities during the overwintering of mud crab Scylla paramamosain

Author:
Junming Zhou; Na Li; Huan Wang; Changkao Mu; Chunlin Wang
Source:
Aquaculture research 2021 v.52 no.7 pp. 3212-3225
ISSN:
1355-557X
Subject:
D-amino-acid oxidase; D-aspartate oxidase; Scylla paramamosain; adenosinetriphosphatase; amino acid metabolism; aquaculture; carbohydrate metabolism; carbonate dehydratase; citrate (si)-synthase; crabs; creatine kinase; energy; energy metabolism; fatty-acid synthase; genes; glutamate-ammonia ligase; glycogen (starch) synthase; hepatopancreas; hexokinase; lipid metabolism; overwintering; research; salinity; sarcosines; transcriptome; transcriptomics
Abstract:
... Overwintering is an important part of the conservation of Scylla paramamosain, and salinity is a primary parameter affecting this species’ metabolism at this particular time. We compared and analysed gene sequences responsible for the metabolism of S. paramamosain at 4‰ versus 25‰ salinities using transcriptome analysis, in order to find out the optimum salinity for S. paramamosain during overwint ...
DOI:
10.1111/are.15167

72. Unraveling Metabolic and Proteomic Features in Soybean Plants in Response to Copper Hydroxide Nanowires Compared to a Commercial Fertilizer

Author:
Sanghamitra Majumdar; Randall W. Long; Jay S. Kirkwood; Anastasiia S. Minakova; Arturo A. Keller
Source:
Environmental science & technology 2021 v.55 no.20 pp. 13477-13489
ISSN:
1520-5851
Subject:
Glycine max; amino acid metabolism; biosynthesis; copper; copper hydroxide; dose response; energy; environmental science; fatty acid metabolism; fertilizers; foliar application; lignin; metabolomics; nanowires; oxidative stress; photosynthesis; plant health; precision agriculture; proteomics; soybeans; stress response
Abstract:
... Mechanistic understanding of the interaction of copper-based nanomaterials with crops is crucial for exploring their application in precision agriculture and their implications on plant health. We investigated the biological response of soybean (Glycine max) plants to the foliar application of copper hydroxide nanowires (CNWs) at realistic exposure concentrations. A commercial copper based-fungici ...
DOI:
10.1021/acs.est.1c00839

73. An atlas of metallome and metabolome interactions and associations with incident diabetes in the Strong Heart Family Study

Author:
Tiffany R. Sanchez; Xin Hu; Jinying Zhao; ViLinh Tran; Nancy Loiacono; Young-Mi Go; Walter Goessler; Shelley Cole; Jason Umans; Dean P. Jones; Ana Navas-Acien; Karan Uppal
Source:
Environment international 2021 v.157 pp. 106810
ISSN:
0160-4120
Subject:
American Indians; amino acid metabolism; antimony; arsenic; biochemical pathways; biomarkers; cadmium; chronic exposure; cohort studies; diabetes; energy; energy metabolism; environment; fatty acids; heart; homeostasis; lead; lipid metabolism; mass spectrometry; metabolites; metabolome; metabolomics; metallome; molybdenum; pathophysiology; phosphates; selenium; tungsten; uranium; urine; xenobiotics; zinc
Abstract:
... Chronic exposure to certain metals plays a role in disease development. Integrating untargeted metabolomics with urinary metallome data may contribute to better understanding the pathophysiology of diseases and complex molecular interactions related to environmental metal exposures. To discover novel associations between urinary metal biomarkers and metabolism networks, we conducted an integrative ...
DOI:
10.1016/j.envint.2021.106810

74. The combination of cinnamaldehyde and kaempferol ameliorates glucose and lipid metabolism disorders by enhancing lipid metabolism via AMPK activation

Author:
Jie Gao; Manqian Zhang; Ruixue Niu; Xue Gu; Erwei Hao; Xiaotao Hou; Jiagang Deng; Gang Bai
Source:
Journal of functional foods 2021 v.83 pp. 104556
ISSN:
1756-4646
Subject:
AMP-activated protein kinase; amino acid metabolism; diabetes; energy; fatty acids; flavor compounds; gluconeogenesis; glucose; glycolysis; kaempferol; lipolysis; metabolomics
Abstract:
... Nutrients in diets, especially functional foods, are beneficial in metabolism-related diseases, such as diabetes. Cinnamaldehyde (CA), a natural flavorant, inhibits glycolysis while enhancing glucose storage. Kaempferol (KP), a flavonol in edible plants, inhibits amino acid metabolism and gluconeogenesis. Whether the combination of CA and KP exerts stronger effects in diabetes needs further invest ...
DOI:
10.1016/j.jff.2021.104556

75. An emerging potential of metabolomics in multiple sclerosis: a comprehensive overview

Author:
Insha Zahoor; Bin Rui; Junaid Khan; Indrani Datta; Shailendra Giri
Source:
Cellular and molecular life sciences 2021 v.78 no.7 pp. 3181-3203
ISSN:
1420-682X
Subject:
amino acid metabolism; animals; biomarkers; drugs; energy; etiology; lipid metabolism; metabolites; metabolome; metabolomics; nervous system; pathogenesis; pathophysiology; patients; sclerosis; therapeutics; tricarboxylic acid cycle
Abstract:
... Multiple sclerosis (MS) is an inflammatory demyelinating disease of the nervous system that primarily affects young adults. Although the exact etiology of the disease remains obscure, it is clear that alterations in the metabolome contribute to this process. As such, defining a reliable and disease-specific metabolome has tremendous potential as a diagnostic and therapeutic strategy for MS. Here, ...
DOI:
10.1007/s00018-020-03733-2

76. iTRAQ-based proteomic analysis reveals positive impacts of arbuscular mycorrhizal fungi inoculation on photosynthesis and drought tolerance in blueberry

Author:
Liang-Xian Gui; Shan-Shan Lu; Qiang Chen; Lu Yang; Jia-Xin Xiao
Source:
Trees 2021 v.35 no.1 pp. 81-92
ISSN:
0931-1890
Subject:
Glomus mosseae; Vaccinium corymbosum; amino acid metabolism; antioxidants; biosynthesis; blueberries; carbon dioxide fixation; carotenoids; chlorophyll; chloroplasts; drought tolerance; energy; photochemistry; photosynthesis; plant growth; proteomics; signal transduction; stress tolerance; vesicular arbuscular mycorrhizae; water stress
Abstract:
... KEY MESSAGE: Our results from iTRAQ-based proteomics extend the understanding of the mechanisms employed by AMF to defend against drought stress in blueberry. Arbuscular mycorrhizal fungi (AMF) can substantially contribute to plant drought tolerance. In this work, south highbush blueberry (Vaccinium corymbosum) ‘O’Neal’ cultivated with or without Funneliformis mosseae inoculation under well-watere ...
DOI:
10.1007/s00468-020-02015-5

77. iTRAQ‐based quantitative proteomic analysis of the effect of heat shock on freeze‐drying of Lactobacillus acidophilus ATCC4356

Author:
Mingxue Liu; Xiaoqun Zeng; Yating He; Chaoran Xia; Lu Cheng; Zhen Wu; Hangzhen Lan; Daodong Pan
Source:
International journal of food science & technology 2021 v.56 no.11 pp. 5569-5580
ISSN:
0950-5423
Subject:
DNA; Lactobacillus acidophilus; amino acid metabolism; energy; food science; freeze drying; gene expression regulation; glycolysis; heat stress; lactic acid; proteomics; survival rate
Abstract:
... In order to investigate the mechanism by which heat shock improves the survival rate of lactic acid bacteria (LAB) following lyophilisation, we performed proteomic analysis based on isobaric tags for relative and absolute quantitation (iTRAQ) on Lactobacillus acidophilus (LA) ATCC4356. Among the 75 differentially expressed proteins (DEPs), 16 and 59 were up‐ and downregulated, respectively, mainly ...
DOI:
10.1111/ijfs.15101

78. iTRAQ‐based quantitative proteomic analysis reveals metabolic changes in overwintering Scylla paramamosain at two different salinities

Author:
Junming Zhou; Na Li; Huan Wang; Chunlin Wang; Changkao Mu
Source:
Aquaculture research 2021 v.52 no.8 pp. 3757-3770
ISSN:
1355-557X
Subject:
NADP-glucose-6-phosphate dehydrogenase; Scylla paramamosain; alkaline phosphatase; amino acid metabolism; aquaculture; carbohydrate metabolism; catalase; crabs; energy; energy metabolism; gene expression; gene expression regulation; glutamate dehydrogenase; hepatopancreas; hexokinase; lactate dehydrogenase; lipid metabolism; overwintering; peptidyl-dipeptidase A; protein content; proteomics; pyruvate kinase; quantitative polymerase chain reaction; research; salinity; superoxide dismutase; trypsin; xanthine; China
Abstract:
... Scylla paramamosain is one of the commercially crucial marine crustaceans belonging to the genus Scylla, which is commonly distributed along the coasts of China. Proteomic data are scarce for the mud crab. Salinity is one of the ecological factors that affect S. paramamosain during overwintering. To understand the energy metabolism mechanism adapted to salinity, we analysed the proteomics of S. pa ...
DOI:
10.1111/are.15221

79. The role of energy, serine, glycine, and 1-carbon units in the cost of nitrogen excretion in mammals and birds

Author:
J. van Milgen
Source:
Animal 2021 v.15 no.5 pp. 100213
ISSN:
1751-7311
Subject:
allantoin; amino acid metabolism; animal production; creatinine; databases; dietary protein; dietary supplements; energy; energy costs; environmental impact; excretion; growth retardation; nitrogen; protein deposition; serine; stoichiometry; urea; uric acid; urine
Abstract:
... The efficiency with which dietary protein is used affects the nitrogen excretion by the animal and the environmental impact of animal production. Urea and uric acid are the main nitrogen excretion products resulting from amino acid catabolism in mammals and birds, respectively. Nitrogen excretion can be reduced by using low-protein diets supplemented with free amino acids to ensure that essential ...
DOI:
10.1016/j.animal.2021.100213

80. The toxicity of selenium and mercury in Suaeda salsa after 7-days exposure

Author:
Ting Liu; Qian Chen; Linbao Zhang; Xiaoli Liu; Chunming Liu
Source:
Comparative biochemistry and physiology 2021 v.244 pp. 109022
ISSN:
1532-0456
Subject:
Suaeda salsa; amino acid metabolism; betaine-aldehyde dehydrogenase; catalase; choline; cysteine; electron transport chain; energy; glycolysis; growth retardation; halophytes; mercury; metabolites; metabolomics; methionine; mitochondria; osmoregulation; pentose phosphate cycle; peroxidase; photosynthesis; protein synthesis; proteomics; ribosomes; selenium; superoxide dismutase; toxicity; tricarboxylic acid cycle
Abstract:
... Mercury is one of the major pollutants in the ocean, selenium causes toxicity beyond a certain limit, but there are few comparative toxic studies between them in halophytes. The study was to investigate the toxic effects of selenium (Se⁴⁺) and mercury (Hg²⁺) in halophyte Suaeda salsa at the level of genes, proteins and metabolites after exposure for 7 days. By integrating the results of proteomics ...
DOI:
10.1016/j.cbpc.2021.109022

81. 1H NMR-based metabolomic analysis of cuttlefish, Sepia pharaonis (Ehrenberg, 1831) exposed to hypoxia stresses and post-anoxia recovery

Author:
Maowang Jiang; Hua Yang; Ruibing Peng; Qingxi Han; Xiamin Jiang
Source:
Science of the total environment 2020 v.726 pp. 138317
ISSN:
0048-9697
Subject:
Sepia pharaonis; adenosine; adenosine triphosphate; alanine; amino acid metabolism; arginine; betaine; biochemical pathways; brain; choline; coastal ecosystems; energy; estuaries; gamma-aminobutyric acid; gills; glutamine; glycogen; hypoxia; lactic acid; metabolites; metabolomics; neurotransmitters; nuclear magnetic resonance spectroscopy; taurine
Abstract:
... Oxygen deficiency (hypoxia and anoxia) is an emerging concern in estuarine and coastal ecosystems worldwide. Previous studies on Mollusca Cephalopoda have focused on the effects of hypoxia stress on physiological performance and survival, but there are few reports on the molecular mechanism, and the application of metabolomics in cephalopods remains unknown. In this study, a ¹H nuclear magnetic re ...
DOI:
10.1016/j.scitotenv.2020.138317

82. Comparative proteomic analysis of mitochondrial proteins from maize CMS‐C sterile, maintainer and restorer anthers

Author:
Huaisheng Zhang; Bin Wang; Bing Li; Yanan Lin; Huili Yang; Dong Ding; Yadong Xue; Jihua Tang
Source:
The plant genome 2020 v.13 no.2 pp. e20022
ISSN:
1940-3372
Subject:
amino acid metabolism; corn; cytoplasmic male sterility; energy; fatty acid metabolism; genes; hybrids; mitochondria; peptides; pollen; proteomics
Abstract:
... The maize C system of cytoplasmic male sterility (CMS) and its fertility restoration gene Rf4 have been widely used for maize hybrid production; however, the underlying mechanism is still uncertain. The sterility factor functions in mitochondria, where it interacts directly or indirectly with the restorer. Mitoproteomics can capture all participants involved in CMS and restoration at the organelle ...
DOI:
10.1002/tpg2.20022

83. Comprehensive dynamic transcriptome analysis at two seed germination stages in maize (Zea mays L.)

Author:
Zanping Han; Bin Wang; Lei Tian; Shunxi Wang; Jun Zhang; ShuLei Guo; Hengchao Zhang; Lengrui Xu; Yanhui Chen
Source:
Physiologia plantarum 2020 v.168 no.1 pp. 205-217
ISSN:
0031-9317
Subject:
Zea mays; abscisic acid; amino acid metabolism; amino acids; auxins; biochemical pathways; corn; crop production; energy; gene expression regulation; gene interaction; genes; gibberellins; grain quality; inbred lines; ribosomes; seed germination; sugars; transcription (genetics); transcription factors; transcriptomics
Abstract:
... Seed germination, as an integral stage of crop production, directly affects Zea mays (maize) yield and grain quality. However, the molecular mechanisms of seed germination remain unclear in maize. We performed comparative transcriptome analysis of two maize inbred lines, Yu82 and Yu537A, at two stages of seed germination. Expression profile analysis during seed germination revealed that a total of ...
DOI:
10.1111/ppl.12944

84. Dietary protein insufficiency: an important consideration in fatty liver disease?

Author:
Isaac Ampong; Adam Watkins; Jorge Gutierrez-Merino; John Ikwuobe; Helen R. Griffiths
Source:
British journal of nutrition 2020 v.123 no.6 pp. 601-609
ISSN:
1475-2662
Subject:
amino acid composition; amino acid metabolism; animal models; biochemical pathways; biogenesis; branched chain amino acids; butyrates; developing countries; dietary protein; energy; fatty liver; insulin resistance; intestinal microorganisms; kwashiorkor; lipogenesis; liver; liver function; low protein diet; mitochondria; nutrients; nutrition risk assessment; obesity; odd chain fatty acids; peroxisomes; propionic acid; protein intake; short chain fatty acids
Abstract:
... Dietary protein insufficiency has been linked to excessive TAG storage and non-alcoholic fatty liver disease (NAFLD) in developing countries. Hepatic TAG accumulation following a low-protein diet may be due to altered peroxisomal, mitochondrial and gut microbiota function. Hepatic peroxisomes and mitochondria normally mediate metabolism of nutrients to provide energy and substrates for lipogenesis ...
DOI:
10.1017/S0007114519003064

85. Effect of Serratia sp. K3 combined with organic materials on cadmium migration in soil-vetiveria zizanioides L. system and bacterial community in contaminated soil

Author:
Huakang Liu; Yanluo Xie; Junjie Li; Guoquan Zeng; Hao Li; Fei Xu; Su Feng; Heng Xu
Source:
Chemosphere 2020 v.242 pp. 125164
ISSN:
0045-6535
Subject:
Chrysopogon zizanioides; Serratia; amino acid metabolism; bacterial communities; biogas; cadmium; carbohydrates; community structure; energy; fertilizers; genes; harvesting; phytoremediation; polluted soils; pollution; rhizosphere; roots; shoots; soil bacteria; straw
Abstract:
... Phytoremediation is an economical strategy to harvest cadmium (Cd) from contaminated soil, but the efficiency of phytoremediation was affected by many factors. This study investigated the potential of Serratia sp. K3 (K3) assisted with straw biogas residue (SBR) or leavening fertilizer (LF) on improving the Cd migration efficiency and micro-environment in soil-vetiveria zizanioides L. system. The ...
DOI:
10.1016/j.chemosphere.2019.125164

86. Effects of reduced crude protein levels, dietary electrolyte balance, and energy density on the performance of broiler chickens offered maize-based diets with evaluations of starch, protein, and amino acid metabolism

Author:
Peter V. Chrystal; Amy F. Moss; Ali Khoddami; Victor D. Naranjo; Peter H. Selle; Sonia Yun Liu
Source:
Poultry science 2020 v.99 no.3 pp. 1421-1431
ISSN:
0032-5791
Subject:
amino acid metabolism; cages; crude protein; diet; digestible amino acids; electrolytes; energy; energy density; feed conversion; free amino acids; growth performance; ileum; jejunum; lysine; males; starch; threonine; weight gain
Abstract:
... The crude protein (CP) content of 4 iso-energetic, maize-based diets containing 11.00 g/kg digestible lysine was reduced in gradations from 200 to 156 g/kg with increasing inclusions of synthetic, or unbound, essential amino acids. A constant dietary electrolyte balance (DEB) of 230 mEq/kg was maintained, but a second 156 g/kg CP diet had a DEB of 120 mEq/kg, and energy densities of the 156 g/kg C ...
DOI:
10.1016/j.psj.2019.10.060

87. Effects of reducing dietary crude protein and whole grain feeding on performance and amino acid metabolism in broiler chickens offered wheat-based diets

Author:
Dafei Yin; Peter V. Chrystal; Amy F. Moss; Sonia Yun Liu; Jianmin Yuan; Peter H. Selle
Source:
Animal feed science and technology 2020 v.260 pp. 114386
ISSN:
0377-8401
Subject:
alanine; amino acid metabolism; asparagine; aspartic acid; broiler chickens; broiler feeding; chicks; crude protein; diet; digestibility; electrolytes; energy; energy density; feed conversion; free amino acids; gizzard; glutamine; glycine (amino acid); growth performance; histidine; ileum; isoleucine; jejunum; leucine; lysine; males; methionine; pancreas; proline; serine; tryptophan; tyrosine; valine; whole grain foods
Abstract:
... A total of 336 off-sex, male Ross 308 chicks were offered seven dietary treatments from 7 to 35 days post-hatch; each treatment was offered to eight replicate cages with six birds per cage. Three wheat-based diets were formulated to declining crude protein (CP) levels of 215, 190 and 165 g/kg but with a constant energy density (12.70 MJ/kg), electrolyte balance (250 mEq/kg) and digestible lysine l ...
DOI:
10.1016/j.anifeedsci.2019.114386

88. Evaluation of the mechanism of Danggui-Shaoyao-San in regulating the metabolome of nephrotic syndrome based on urinary metabonomics and bioinformatics approaches

Author:
Yunlai Wang; Shengnan Fan; Mo Yang; Gaoxiang Shi; Siyao Hu; Dengke Yin; Yazhong Zhang; Fan Xu
Source:
Journal of ethnopharmacology 2020 v.261 pp. 113020
ISSN:
0378-8741
Subject:
Oriental traditional medicine; amino acid metabolism; animal models; biochemical pathways; bioinformatics; blood circulation; diuresis; doxorubicin; energy; energy metabolism; enzyme inhibitors; enzymes; mass spectrometry; metabolites; metabolome; metabolomics; nephrotic syndrome; protective effect; protein phosphorylation; rats; therapeutics; ultra-performance liquid chromatography
Abstract:
... Danggui-Shaoyao-San (DSS), a well-known classic Traditional Chinese medicine (TCM) formula for enhancing Qi (vital energy and spirit), invigorating blood circulation and promoting diuresis, has been widely used in the treatment of nephrotic syndrome (NS). Previously, we have reported some protective effects of DSS against NS, but the in-depth mechanisms remain unclear.In this study, an ultra perfo ...
DOI:
10.1016/j.jep.2020.113020

89. Exploring the mechanism underlying the cardioprotective effect of shexiang baoxin pill on acute myocardial infarction rats by comprehensive metabolomics

Author:
Gaosong Wu; Linlin Chen; Yu Gu; Ying Hong; Junli Ma; Ningning Zheng; Jing Zhong; Ai-Jun Liu; Lili Sheng; Weidong Zhang; Houkai Li
Source:
Journal of ethnopharmacology 2020 v.259 pp. 113001
ISSN:
0378-8741
Subject:
5-hydroxyindoleacetic acid; Oriental traditional medicine; adenosine; amino acid metabolism; animal models; biochemical pathways; blood serum; butyrates; cardioprotective effect; coronary vessels; databases; echocardiography; energy; feces; heart; intestinal microorganisms; lipids; liquid chromatography; mass spectrometry; metabolites; metabolomics; myocardial infarction; phenylalanine; rats; therapeutics; urine
Abstract:
... Shexiang Baoxin Pill (SBP) is a commercial Chinese medicine included in the Chinese Pharmacopoeia with well-established cardiovascular protect effect in clinic. However, the mechanism of SBP underlying protective effect on cardiovascular disease has not been clearly elucidated yet.We aimed to investigate the underlying protective mechanisms of SBP on an acute myocardial infarction (AMI) rat model ...
DOI:
10.1016/j.jep.2020.113001

90. Functional prediction, characterization, and categorization of operome from Acetoanaerobium sticklandii DSM 519

Author:
C. Sangavai; R. Prathiviraj; P. Chellapandi
Source:
Anaerobe 2020 v.61 pp. 102088
ISSN:
1075-9964
Subject:
abiotic stress; amino acid metabolism; amino acids; anaerobes; bacteria; biochemical pathways; biofuels; bioinformatics; biosynthesis; carbon; cattle; cell cycle; energy; gene expression; genome; niches; prediction; proteins
Abstract:
... Acetoanaerobium sticklandii DSM 519 is a hyper-ammonia producing anaerobic bacterium that can be able utilizes amino acids as sole carbon and energy sources for its growth and energetic metabolism. A lack of knowledge on its molecular machinery and 30.5% conserved hypothetical proteins (HPs; operome) hinders the successful utility in biofuel applications. In this study, we have predicted, characte ...
DOI:
10.1016/j.anaerobe.2019.102088

91. Genome-Scale Metabolic Modeling Reveals Metabolic Alterations of Multidrug-Resistant Acinetobacter baumannii in a Murine Bloodstream Infection Model

Author:
Jinxin Zhao; Yan Zhu; Jiru Han; Yu-Wei Lin; Michael Aichem; Jiping Wang; Ke Chen; Tony Velkov; Falk Schreiber; Jian Li
Source:
Microorganisms 2020 v.8 no.11 pp. -
ISSN:
2076-2607
Subject:
Acinetobacter baumannii; amino acid metabolism; assays; biochemical pathways; biogenesis; biosynthesis; blood flow; cell membranes; energy; environment; fatty acids; genes; gluconeogenesis; human health; infection; lipopolysaccharides; metabolites; mice; microorganisms; models; multiple drug resistance; mutants; nutrient utilization; pathogens; peptidoglycans; prediction; therapeutics; transcriptomics; transposons
Abstract:
... Multidrug-resistant (MDR) Acinetobacter baumannii is a critical threat to human health globally. We constructed a genome-scale metabolic model iAB5075 for the hypervirulent, MDR A. baumannii strain AB5075. Predictions of nutrient utilization and gene essentiality were validated using Biolog assay and a transposon mutant library. In vivo transcriptomics data were integrated with iAB5075 to elucidat ...
DOI:
10.3390/microorganisms8111793

92. Genomic and Transcriptomic Analysis for Identification of Genes and Interlinked Pathways Mediating Artemisinin Resistance in Leishmania donovani

Author:
Sushmita Ghosh; Aditya Verma; Vinay Kumar; Dibyabhaba Pradhan; Angamuthu Selvapandiyan; Poonam Salotra; Ruchi Singh
Source:
Genes 2020 v.11 no.11 pp. -
ISSN:
2073-4425
Subject:
DNA; DNA microarrays; Leishmania donovani; amino acid metabolism; amino acid transporters; artemisinin; cathepsin L; chromosomes; drug resistance; energy; gene expression regulation; genes; genomics; lipids; protein synthesis; surface proteins; therapeutics; toxicity; transcriptomics; trisomics; visceral leishmaniasis
Abstract:
... Current therapy for visceral leishmaniasis (VL), compromised by drug resistance, toxicity, and high cost, demands for more effective, safer, and low-cost drugs. Artemisinin has been found to be an effectual drug alternative in experimental models of leishmaniasis. Comparative genome and transcriptome analysis of in vitro-adapted artesunate-resistant (K133AS-R) and -sensitive wild-type (K133WT) Lei ...
DOI:
10.3390/genes11111362

93. High fat diet incorporated with meat proteins changes biomarkers of lipid metabolism, antioxidant activities, and the serum metabolomic profile in Glrx1⁻/⁻ mice

Author:
Muhammad Ijaz Ahmad; Muhammad Umair Ijaz; Muzahir Hussain; Iftikhar Ali Khan; Noreen Mehmood; Sultan Mehmood Siddiqi; Congcong Liu; Di Zhao; Xinglian Xu; Guanghong Zhou; Chunbao Li
Source:
Food & function 2020 v.11 no.1 pp. 236-252
ISSN:
2042-650X
Subject:
alanine transaminase; amino acid metabolism; antioxidant activity; aspartate transaminase; bile acids; biochemical pathways; biomarkers; blood serum; casein; cholesterol; cholesterol metabolism; diabetes; droplets; energy; fatty liver; fish; gene expression; glutathione; high fat diet; histology; linoleic acid; males; meat consumption; meat protein; metabolites; metabolome; mice; mutton; oxidative stress; processed meat; sphinganine; triacylglycerols
Abstract:
... Red and processed meat consumption has been associated with oxidative stress, diabetes and non-alcoholic fatty liver disease (NAFLD). This study was aimed at exploring the effects of high-fat meat protein diets on potential metabolite biomarkers in Glrx1⁻/⁻ mice, a well-documented mouse model to study NAFLD. Male Glrx1⁻/⁻ mice were fed a control diet with 12% energy (kcal) from fat, a high-fat die ...
DOI:
10.1039/c9fo02207d

94. Hyperosmotic Adaptation of Pseudomonas protegens SN15-2 Helps Cells to Survive at Lethal Temperatures

Author:
Xiaobing Wang; Danyan Tang; Wei Wang
Source:
Biotechnology and bioprocess engineering 2020 v.25 no.3 pp. 403-413
ISSN:
1226-8372
Subject:
Gram-negative bacteria; Pseudomonas protegens; amino acid metabolism; biological control; cations; cell movement; energy; freezing; gene ontology; genes; osmotolerance; potassium; proline; sequence analysis; sodium chloride; temperature; transcriptomics; trehalose
Abstract:
... Pseudomonas protegens is a gram-negative bacterium with an excellent biological control effect. Compared to standard cells growing in NaCl-free media, the ability of the hyperosmotic cells (450 mM NaCl) to resist high temperatures and freezing was significantly improved. It is of great significance to apply the P. protegens to elaborate on the hyperosmotic adaptation mechanism. RNA-seq was used to ...
DOI:
10.1007/s12257-019-0430-x

95. Integrated adrenal and testicular metabolomics revealed the protective effects of Guilingji on the Kidney-Yang deficiency syndrome rats

Author:
Ke Du; Xiao-xia Gao; Yan Feng; Jing Li; Hui Wang; Si-lin Lv; Pei-yi Wang; Bin Zhang; Xue-mei Qin
Source:
Journal of ethnopharmacology 2020 v.255 pp. 112734
ISSN:
0378-8741
Subject:
Oriental traditional medicine; adrenal glands; amino acid metabolism; biosynthesis; body weight; cortisol; energy; herbal medicines; histology; lipid metabolism; lipids; metabolomics; models; multivariate analysis; nucleotides; pharmacodynamics; protective effect; rats; steroid hormones; testes; tissues; vitamins
Abstract:
... Guilingji (GLJ) is a well-known traditional Chinese medicine (TCM) prescription for the treatment of Kidney-Yang deficiency syndrome (KYDS).This study aimed to address the protective effects of GLJ against KYDS in rats with pharmacodynamic indicators and target tissues (adrenal gland and testis) metabolomics.The rats were injected intraperitoneally (i.p) hydrocortisone to simulate KYDS and adminis ...
DOI:
10.1016/j.jep.2020.112734

96. Integrated analysis of physiological, transcriptomic and metabolomic responses and tolerance mechanism of nitrite exposure in Litopenaeus vannamei

Author:
Jie Xiao; Qing-Yun Liu; Jing-Hao Du; Wei-Lin Zhu; Qiang-Yong Li; Xiu-Li Chen; Xiao-Han Chen; Hong Liu; Xiao-Yun Zhou; Yong-Zhen Zhao; Huan-Ling Wang
Source:
Science of the total environment 2020 v.711 pp. 134416
ISSN:
0048-9697
Subject:
Litopenaeus vannamei; amino acid metabolism; antioxidant enzymes; apoptosis; aquatic animals; aquatic environment; cytoskeleton; energy; fatty acids; gas exchange; gene expression; gene expression regulation; genes; hemocyanin; hepatopancreas; histology; hypoxia; hypoxia-inducible factor 1; immune system; lipid metabolism; metabolome; metabolomics; nitrites; risk factors; stress response; transcriptome; transcriptomics
Abstract:
... Nitrite accumulation in aquatic environments is a potential risk factor that disrupts multiple physiological functions in aquatic animals. In this study, the physiology, transcriptome and metabolome of the control group (LV-C), nitrite-tolerance group (LV-NT) and nitrite-sensitive group (LV-NS) were investigated to identify the stress responses and mechanisms underlying the nitrite tolerance of Li ...
DOI:
10.1016/j.scitotenv.2019.134416

97. Metabolic characterisation of eight Escherichia coli strains including "Big Six" and acidic responses of selected strains revealed by NMR spectroscopy

Author:
Lin Chen; Xue Zhao; Ji'en Wu; Qin Liu; Xinyi Pang; Hongshun Yang
Source:
Food microbiology 2020 v.88 pp. 103399
ISSN:
0740-0020
Subject:
Escherichia coli O157; Escherichia coli O26; acid tolerance; adenosine; alpha-ketoglutaric acid; amino acid metabolism; antiporters; arginine; energy; fumaric acid; glutamic acid; lysine; metabolites; nuclear magnetic resonance spectroscopy; principal component analysis; quantitative polymerase chain reaction; virulent strains
Abstract:
... The metabolic diversity of Escherichia coli strains (non-pathogenic E. coli ATCC 25922, and pathogenic E. coli O157:H7, O26:H11, O45:H2, O103:H11, O111, O121:H19, and O145) was tested using nuclear magnetic resonance. Based on two representative two-dimensional ¹H–¹³C spectra, 38 metabolites were identified in E. coli intracellular samples. Principal component analysis indicated that metabolites i ...
DOI:
10.1016/j.fm.2019.103399

98. Metabolic impairments, metal traffic, and dyshomeostasis caused by the antagonistic interaction of cadmium and selenium using organic and inorganic mass spectrometry

Author:
Gema Rodríguez-Moro; Francisco Navarro Roldán; Rocío Baya-Arenas; Ana Arias-Borrego; Belén Callejón-Leblic; José Luis Gómez-Ariza; Tamara García-Barrera
Source:
Environmental science and pollution research international 2020 v.27 no.2 pp. 1762-1775
ISSN:
0944-1344
Subject:
amino acid metabolism; arsenic; atomic absorption spectrometry; biochemical pathways; blood serum; brain; cadmium; energy; excretion; free fatty acids; gas chromatography-mass spectrometry; health effects assessments; homeostasis; humans; kidneys; liver; lungs; metabolomics; mice; oxidative stress; pollutants; selenium; testes; toxicity; traffic
Abstract:
... Cadmium (Cd) has become one of the most important environmental pollutants in the world, derived from natural and industrial sources, which is known to be accumulated in the human body, producing serious health effects. On the other hand, Selenium (Se) is an essential element for mammals, which is well known for its antagonistic interaction against Cd toxicity, such as the prevention of oxidative ...
DOI:
10.1007/s11356-019-06573-1

99. Metabolic mechanism of the mud crab (Scylla paramamosain) adapting to salinity sudden drop based on GC-MS technology

Author:
Hongzhi Yao; Xing Li; Lei Tang; Huan Wang; Chunlin Wang; Changkao Mu; Ce Shi
Source:
Aquaculture reports 2020 v.18 pp. 100533
ISSN:
2352-5134
Subject:
ABC transporters; Scylla paramamosain; amino acid metabolism; aspartic acid; carbohydrate metabolism; crabs; death; energy; energy metabolism; euryhaline species; free amino acids; fructose; fructose 6-phosphate; gas chromatography-mass spectrometry; gene expression regulation; glucose 6-phosphate; glutamic acid; glycine (amino acid); lactic acid; marine crustaceans; metabolites; osmoregulation; pentose phosphate cycle; pyruvic acid; ribose; salinity; serine; tagatose; taurine; threonine; valine; vitamins
Abstract:
... Salinity impacts the respiratory metabolism, growth, and survival of marine crustaceans. Although the S. paramamosain is a euryhaline species, the sudden drop in salinity often causes individual death. The study aimed to reveal the adaptive mechanism of S. paramamosain in response to a sudden drop from 23‰ to 3‰ in salinity based on GC–MS data. We identified 1264 metabolites, and 437 were differen ...
DOI:
10.1016/j.aqrep.2020.100533

100. Metabolic profiles of Oncomelania hupensis after molluscicidal treatment: Carbohydrate metabolism targeted and energy deficiency

Author:
Tao Xiong; Ni Jiang; Sha Xu; Shi Zhu Li; Yan Zhang; Xing Jian Xu; Hui Fen Dong; Qin Ping Zhao
Source:
Acta tropica 2020 v.210 pp. 105580
ISSN:
0001-706X
Subject:
Oncomelania; Schistosoma japonicum; World Health Organization; aerobiosis; alanine; amino acid metabolism; cell structures; choline; death; energy; ethanolamine; gene expression regulation; gluconeogenesis; glucose; glycolysis; humans; hysteresis; intermediate hosts; liver; maltose; mathematical models; metabolites; metabolomics; molluscicides; muscles; niclosamide; nucleic acids; oxidative phosphorylation; schistosomiasis; snails; succinic acid; toxicity; transamination; transcriptomics; unigenes; wettable powders; China
Abstract:
... Oncomelania hupensis is the intermediate host of Schistosoma japonicum, one of the Schistosoma species that can cause human schistosomiasis. Molluscicidal treatment remains the primary means to control snail. Niclosamide is the only molluscicide recommended by the World Health Organization, and it has been used throughout schistosomiasis-endemic areas in China for almost 30 years. In our previous ...
DOI:
10.1016/j.actatropica.2020.105580