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... With the rapid development of hydropower facility construction, the total dissolved gas (TDG) generated by dam discharge is seriously threatening the survival of fish and has become an ecological environmental issue of global concern. However, how TDG affects fish physiology and the underlying molecular mechanism remain poorly known. In this study, Acipenser dabryanus, an ancient living fossil tha ...
... Mechanical damage caused by vibration during transportation can destroy organization structure and reduce the fruit quality. The objective was to reveal the mechanism of hypobaric treatment on energy metabolism in vibration-injured 'Huangguan' pears based on metabolomics. Results showed that hypobaric treatment delayed the decline of adenosine triphosphate (ATP) content, energy charge (EC), H⁺-ATP ...
... Nonalcoholic fatty liver disease (NAFLD) is one of the major causes of hepatocellular carcinoma (HCC). Although the intracellular cholesterol accumulation has been demonstrated to regulate the gene expression responsible for steatohepatitis, the role played by cholesterol in the development of NAFLD-associated HCC has not been fully elucidated. In this study, using microarray analysis, we investig ...
... Pre-slaughter transport stress could induce multiple comprehensive variations in physiological and metabolic parameters of broilers. However, the entire metabolomics of pre-slaughter transport stress and supplementation of exogenous energy regulatory substances on broilers is still poorly understood. The metabolome characteristics of broilers subjected to 3 h pre-slaughter transport stress combine ...
Luiza Medeiros; Raíza Azevedo; Jade Riet; Bruna Nornberg; Arthur Cardoso; Victor Torres Rosas; Marcelo Borges Tesser; Virgínia F. Pedrosa; Luis A. Romano; Wilson Wasielesky Jr; Luis Fernando Marins
... Cyanobacteria are a rich source of nutrients in addition to producing bioactive compounds capable of stimulating the immune system of hosts. Also, they can be manipulated with relative ease to produce heterologous proteins. In the present study, a strain of Synechococcus elongatus (PCC7942) was manipulated to produce a prokaryotic β-glucosidase. This strain was added to the diet of Pacific white s ...
... Chinese traditional herbs are used widely as feed supplements to improve the immune response and antioxidant capacity of livestock. Twenty early-weaned 4-month-old yak calves (72.3 ± 3.65 kg) were divided randomly into four groups (n = 5 per group); three groups were provided with supplementary 80 mL/kg DMI of the root water extracts of either Angelica sinensis, Codonopsis pilosula or Glycyrrhiza ...
DENG Jun-cai; LI Xiao-man; XIAO Xin-li; WU Hai-jun; YANG Cai-qiong; LONG Xi-yang; ZHANG Qi-hui; IQBAL Nasir; WANG Xiao-chun; YONG Tai-wen; DU Jun-bo; YANG Feng; LIU Wei-guo; ZHANG Jing; WU Xiao-ling; WU Yu-shan; YANG Wen-yu; LIU Jiang
... Excessive rainfall provides a favorable condition for field mold infection of plants, which triggers field mold (FM) stress. If FM stress occurs during the late maturation stage of soybean seed, it negatively affects seed yield and quality. To investigate the responses of soybean seed against FM stress and identify the underlying biochemical pathways involved, a greenhouse was equipped with an art ...
... The effect of dietary digestible protein to digestible energy (DP/DE) ratio on the growth performance and metabolic responses of Nile tilapia juveniles was evaluated. Five experimental diets were formulated with increasing DP/DE ratio (13.2, 15.5, 18.4, 20.0, and 22.6 mg kJ⁻¹) and fed to eight replicates of Nile tilapia (initial body weight of 9.3 g) for 110 days. The apparent digestibility coeffi ...
... Climate change has numerous effects on poultry that result in welfare concerns and economic losses in agricultural industries. However, the mechanisms underlying the acclimation to heat stress in poultry have not been comprehensively defined. Therefore, identifying associated patterns of gene regulation and understanding the molecular mechanisms of acclimation to a warmer environment will provide ...
... Dinotefuran is a chiral insecticide widely used to control Nilaparvata lugens in agriculture. However, little is known about the toxic effects of dinotefuran enantiomers on aquatic organisms. In this study, zebrafish were exposed to 1.00 and 10.00 mg/L dinotefuran enantiomers for 96 h, after which multivariate pattern recognition, metabolite identification, and pathway analysis were performed. Pri ...
... Castor (Ricinus communis L.) is an important oilseed crop worldwide, and is also considered as one of the most promising plants for alkali-affected soil amelioration in the northeast of China. Seed germination plays a vital role in seedling establishment, and is always sensitive to alkali stress. However, little is known about the dynamic changes and regulatory mechanisms of gene expression and me ...
... Hyperlipidemia with fat accumulation and weight gain causes metabolic diseases and endangers human body health easily which is accompanied by metabolic abnormalities and intestinal flora disorders. In this study, the kidney bean fermented broth (KBF) was used in rats that were fed a high-fat diet to induce hyperlipidemia in order to subsequently analyse the serum metabolomics and gut microbiota mo ...
Oihane E. Albóniga; Daniel Jiménez; Matilde Sánchez-Conde; Pilar Vizcarra; Raquel Ron; Sabina Herrera; Javier Martínez-Sanz; Elena Moreno; Santiago Moreno; Coral Barbas; Sergio Serrano-Villar
... Despite the scientific and human efforts to understand COVID-19, there are questions still unanswered. Variations in the metabolic reaction to SARS-CoV-2 infection could explain the striking differences in the susceptibility to infection and the risk of severe disease. Here, we used untargeted metabolomics to examine novel metabolic pathways related to SARS-CoV-2 susceptibility and COVID-19 clinic ...
... Influenza is a global public health issue leading to widespread morbidity and mortality with devastating economic loss annually. Madin-Darby Canine Kidney (MDCK) cell line has been a major cell line for influenza vaccine applications. Though many details of the host metabolic responses upon influenza A virus (IAV) infection have been documented, little is known about the metabolic reprogramming fe ...
... This study simulated the effects of diving activities on the physiology, enzymatic, and transcriptional responses of Acropora microphthalma. Touching had less impact on Fv/Fm, but a few zooxanthellae were decreased and minor MDA was elevated. Caspase 3 was activated to remove damaged cells, and SOD was increased to alleviate oxidative damage. Under double or triple diving stress, we observed mass ...
Calvin cycle; Vitis vinifera; acclimation; amino acid metabolism; climate change; cultivars; drought; drought tolerance; energy; new species; osmotolerance; photorespiration; protective effect; protein synthesis; proteomics; signal transduction; viticulture; water stress
Abstract:
... Drought is one of the major environmental constraints threatening viticulture worldwide. Therefore, it is critical to reveal the molecular mechanisms underlying grapevine (Vitis vinifera L.) drought stress tolerance useful to select new species with higher tolerance/resilience potentials. Drought-tolerant Tunisian local grapevine cultivar Razegui was exposed to water deficit for 16days. Subsequent ...
... Pistia stratiotes is a cadmium (Cd) hyperaccumulating plant with strong bioaccumulation and translocation capacity for Cd. A hydroponic experiment was used to evaluate the combined effect of Zinc (Zn) and Cd at different concentrations on leaf growth and metabolism of P. stratiotes. This study revealed the physiological defense and metabolic strategy of responses to Zn–Cd co-pollution. With the Zn ...
... KEY MESSAGE: Herein, the inoculation with strain wp-6 promoted the growth of wheat seedlings by improving the energy production and conversion of wheat seedlings and alleviating salt stress. Soil salinization decreases crop productivity due to high toxicity of sodium ions to plants. Plant growth-promoting rhizobacteria (PGPR) have been demonstrated to alleviate salinity stress. However, the mechan ...
... Abundant natural gas reserves, along with increased biogas production, have prompted recent interest in harnessing methane as an industrial feedstock for the production of liquid fuels and chemicals. Methane can either be used directly for fermentation or first oxidized to methanol via biological or chemical means. Methanol is advantageous due to its liquid state under normal conditions. Methylotr ...
... Although amino acid (AA) metabolism is basis of bacterial activities, unique characteristics of its response to decreased temperatures are not fully understood. Achieving nitrogen removal rate of 130–150 mg N/ (L∙d), metabolic differences of anammox consortia between 35 °C and four decreased temperatures (15–30 °C) were revealed respectively. 0-11.4-fold abundance variation of marker metabolites e ...
... Rumex gmelinii Turcz. (RGT) is a medicinal plant of the genus Rumex, family Polygonaceae. Our research group isolated an endophytic fungus, Plectosphaerella cucumerina (Strain J-G) from RGT, which could significantly promote host growth when co-cultured with host seedlings. In this study, we used transcriptome analysis and verification experiments to explore the molecular mechanisms underlying thi ...
... Spartina alterniflora, a highly invasive plant, has caused a serious threat to ecosystem biodiversity and economic development in coastal areas of many countries. In this study, the allelopathic effect of Ulva prolifera extracts on seed germination and seedling growth of S. alterniflora was studied. The results showed that three different treatments (water, methanol and ethyl acetate extract) coul ...
... To assess the effects of three specific exercise training modes, aerobic exercise (A), resistance training (R) and autonomous climbing (AC), aimed at proposing a cross-training method, on improving the physical, molecular and metabolic characteristics of mice without many side effects.Seven-week-old male mice were randomly divided into four groups: control (C), aerobic exercise (A), resistance tra ...
... This study aimed to explore the effect of Dendrobium officinale (DO) on the diversity of intestinal mucosal flora in high-fat diet mice and provided an experimental basis for the development and research of DO and its series products. Twenty-four mice were randomly assigned to four equal groups of six mice, namely the control (bcm) group, model (bmm) group, Dendrobium officinale (bdm) group, and p ...
... Streptococcus iniae is known as a most common pathogen in rainbow trout farms, which its pathogenicity depends strongly on temperature. This study was performed to identify the metabolic responses of the Streptococcus iniae at different temperatures (22, 18, 14, 10°C). For this purpose, the samples of the bacteria were cultured at experimental temperatures in three replicates. After metabolite ext ...
... Sparassis crispa (S.crispa), an edible mushroom, is widely used as a natural medicine due to its excellent pharmacological activities, including antitumor, anti-angiogenic, and immunomodulatory activities. In the current study, the digestion and fermentation characteristics of an S.crispa polysaccharide (SCP-1) were investigated by the in vitro simulated models. Our results revealed that SCP-1 was ...
... Even though breast cancer is the most diagnosed cancer among women, treatments are not always successful in preventing its progression. Recent studies suggest that hypoxia and the extracellular matrix (ECM) are important in altering cell metabolism and tumor metastasis. Therefore, the aim of this review is to study the crosstalk between hypoxia and the ECM and to assess their impact on breast canc ...
... Due to anthropogenic activities that have increased global climate change and nutrient discharges, severe hypoxic events have frequently occurred in coastal waters in recent years. Relying on coastal waters, the aquaculture area has suffered ecological and economic losses caused by hypoxia, especially in summer. In this study, to investigate the stress resistance of the Pacific abalone Haliotis di ...
... Fungi have been reported to be the dominant eukaryotic group in anoxic sub‐seafloor sediments, but how fungi subsist in the anoxic sub‐marine sedimental environment is rarely understood. Our previous study demonstrated that the fungus, Schizophyllum commune 20R‐7‐F01 isolated from a ~2 km sediment below the seafloor, can grow and produce primordia in the complete absence of oxygen with enhanced pr ...
... BACKGROUND: Cymbidium goeringii, belonging to the Orchidaceae family, is an important ornamental plant with striking petals and lips. Extremely diversified floral patterns and morphologies make C. goeringii good research material to examine floral development of orchids. However, no floral organ-specific protein has been identified yet. To screen floral development associated proteins, four proteo ...
... Nattokinase is a serine protease which is produced by Bacillus subtilis and exhibits vigorous fibrinolytic activity. Nattokinase is useful for preventing and treating thromboembolic diseases. In this study, the effect of ultraviolet combined ⁶⁰Co-γ ray on B. subtilis were investigated, and the mechanism of NK production enhancement was elucidated at molecular level. There was obvious differences o ...
... Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Infection of the silkworm, Bombyx mori, by Bombyx mori nucleopolyhedrovirus (BmNPV), has been studied extensively in the past to unravel interactions between baculoviruses and their lepidopteran hosts. To understand the interaction between the host metabolic responses ...
... Metabolic reprogramming is a hallmark of cancer and has proven to be critical in viral infections. Metabolic reprogramming provides the cell with energy and biomass for large-scale biosynthesis. Based on studies of the cellular changes that contribute to metabolic reprogramming, seven main hallmarks can be identified: (1) increased glycolysis and lactic acid, (2) increased glutaminolysis, (3) incr ...
... Heat stress impairs growth performance and alters body protein and amino acid metabolism. This study was investigated to explore how body protein and amino acid metabolism changed under heat stress (HS) and the stress adaptation mechanism. A total of 144 broilers (28 d old) were divided into 3 treatment groups for 1 wk: HS group (32°C), normal control group (22°C), and pair-feeding group (22°C). W ...
... The transition from pregnancy to lactation is the most challenging period for high-producing dairy cows. The liver plays a key role in biological adaptation during the peripartum. Prior works have demonstrated that hepatic glucose synthesis, cholesterol metabolism, lipogenesis, and inflammatory response are increased or activated during the peripartum in dairy cows; however, those works were limite ...
... The “prise de mousse” stage during sparkling wine elaboration by the traditional method (Champenoise) involves a second fermentation in a sealed bottle followed by a prolonged aging period, known to contribute significantly to the unique organoleptic properties of these wines. During this stage, CO₂ overpressure, nutrient starvation and high ethanol concentrations are stress factors that affect ye ...
... Whilst carbohydrates are the principal respiratory substrate in plants, under certain circumstances amino acids may be catabolised in order to produce energy, reducing equivalents and precursors for biosynthesis. This situation may occur during the phase of reserve mobilisation in seedlings, and this period of development therefore represents an opportunity to identify which amino acids are in fac ...
... Mudflat shellfish have evolved well-adapted strategies for coping with dynamic environmental fluxes and stressful conditions, including oxygen availability. The Manila clams Ruditapes philippinarum are worldwide cultured shellfish in marine intertidal zone, which usually encounter great risk of acute hypoxia exposure in coastal habitats. To reveal the effects of acute hypoxia on metabolic changes ...
... Bermudagrass is a widely used warm-season turfgrass species with superior stress tolerance. Due to limited genome information, detailed stress response mechanisms of bermudagrass were not extensively characterized. In this study, we compared transcriptomic and proteomic level changes of bermudagrass after treatments with drought, salt and submergence. The results showed that 31.9–61.4% unigenes we ...
... BACKGROUND: The idiosyncratic hepatotoxicity of Polygonum multiflorum (PM) has attracted considerable interest, but the idiosyncratically hepatotoxic components and endogenous metabolite changes resulting from idiosyncratic hepatotoxicity of PM are not well understood. The aim of this study was to identify the idiosyncratically hepatotoxic components and potential endogenous metabolic biomarkers f ...
... Arsenic ions (As³⁺) have been recognized as a hazard that threatens the health of humans. Metallothionein (MT) rich in cysteine may provide favorable binding sites for chelation of As³⁺. However, the influence of MT on As³⁺-induced toxicity and the underlying mechanism are poorly understood, especially at the metabolic level. Herein, the effects of MT on As³⁺-induced toxicity were evaluated. Cell ...
... Gracilariopsis lemaneiformis, an economically important red seaweed in China, is currently undergoing high-temperature (HT) stress due to global warming. To alleviate summertime HT stress, algae can acquire resistance through metabolism reprogramming and modulation of stress-related gene expression. γ-Aminobutyric acid (GABA), a non-protein four‑carbon amino acid, plays an important role in regula ...
... Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pullets were used, in which 30 pullets were randomly assigned to one of 10 unique growth trajectories ...
... BACKGROUND: Aphid attack induces defense responses in plants activating several signaling cascades that led to the production of toxic, repellent or antinutritive compounds and the consequent reorganization of the plant primary metabolism. Pepper (Capsicum annuum L.) leaf proteomic response against Myzus persicae (Sulzer) has been investigated and analyzed by LC-MS/MS coupled with bioinformatics t ...
... Because reproduction requires a considerable energy investment that presents metabolic challenges to animals, there is growing scientific interest in the effects of reproduction on metabolism. Here, the proteome of the hepatopancreas from female and male clams (Meretrix petechialis) before and after spawning was obtained using iTRAQ-based proteome analysis. Forty-two DEPs and 37 DEPs were detected ...
... As an important ruminant on the Qinghai-Tibet Plateau, Tibetan sheep can maintain their population reproduction rate in the harsh high-altitude environment of low temperature and low oxygen, which relies on their special plateau adaptations mechanism that they have formed for a long time. Microbiomes (known as “second genomes”) are closely related to the nutrient absorption, adaptability, and heal ...
... Engineered nanoparticles including ZnO nanoparticles (nZnO) are important emerging pollutants in aquatic ecosystems creating potential risks to coastal ecosystems and associated biota. The toxicity of nanoparticles and its interaction with the important environmental stressors (such as salinity variation) are not well understood in coastal organisms and require further investigation. Here, we exam ...
... Rushan is a traditional dairy product consumed by the Bai people in the Yunnan Province of China, and its production still follows the traditional procedure of backslopping. However, how the microbial composition of raw materials and processing shape the microorganisms in rushan have not been systemically reported. In this study, high-throughput sequencing technique was applied to analyze the micr ...
... The present study aimed to evaluate, in gilthead sea bream, the effect of dietary protein:energy (P/E) ratio and non‐protein energy (NPE) source on the post‐prandial profile of plasmatic parameters, hepatic energy reserves, enzymatic activities of digestive enzymes and hepatic key enzymes activities of the glycolytic (HK and GK), lipogenic (G6PDH and FAS), gluconeogenic (FBPase) and amino acid (GD ...
... Overwintering is an important part of the conservation of Scylla paramamosain, and salinity is a primary parameter affecting this species’ metabolism at this particular time. We compared and analysed gene sequences responsible for the metabolism of S. paramamosain at 4‰ versus 25‰ salinities using transcriptome analysis, in order to find out the optimum salinity for S. paramamosain during overwint ...